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  • posttranslational
  • Cloning of the cDNA and heterologous expression of fluorescent protein in a wide variety of organisms indicate that this unique posttranslational modification must be either spontaneous or dependent only on ubiquitous enzymes and reactants. (pnas.org)
  • In contrast to fluorescent proteins which form their chromophore through posttranslational modifications of the polypeptide chain, phytochromes bind an external ligand (in this case, biliverdin), and successful imaging of the first bacteriophytochrome-based probe required addition of the exogenous biliverdin. (wikipedia.org)
  • excitation
  • Although this wild-type GFP was fluorescent, it had several drawbacks, including dual peaked excitation spectra, poor photostability and poor folding at 37°C. (wikidoc.org)
  • Molecule
  • A protein molecule is an intricate system whose function is highly sensitive to small external perturbations. (rcsb.org)
  • The X-ray structures of around 30 different DARPin complexes demonstrate their ability to facilitate crystallization of their target proteins by restricting flexibility and preventing undesired interactions of the target molecule. (rcsb.org)
  • Chalfie
  • Correctly recognizing that the protein could glow on its own, Chalfie expressed it in his experimental organism of choice, the transparent roundworm C. elegans . (arstechnica.com)
  • florescent protein
  • A hybrid between a highly regenerative diploid clone (BARD 1-3) of Solanum phureja and haploid inducer IVP 101 was transformed with Agrobacterium tumefaciens strain 4404 containing plasmid pHB2892 with genes for green florescent protein (GFP) and kanamycin resistance. (vt.edu)
  • make proteins
  • Such nanoparticles - materials that range roughly in size from 1/1,000 to 1/100,000 the diameter of a human hair - contain a mixture of biological machinery needed to make proteins based on DNA sequences. (nbcnews.com)
  • kinase
  • The cytomegalovirus (CMV) and phosphoglycerate kinase (PGK) promoters are frequently used for expression of exogenous proteins in most cell types. (biomedcentral.com)
  • Then, the effect of the upstream gene phospholipase A2 gamma ( PLA2-3 ) on LPC (18:0), as well as the effect of furosine (100 mg/L) on the receptor-interacting serine/threonine-protein kinase (RIPK)1/RIPK3/mixed lineage kinase domain-like protein (MLKL) pathway and inflammatory factors, was determined in liver tissue and primary hepatocytes. (mdpi.com)
  • biomolecule
  • These results have significant implications for protein function prediction and biomolecule design and engineering, because they suggest methods to tune protein function by modification of the protein scaffold. (rcsb.org)
  • LEDs
  • The gel is vacuum dried and becomes a rubbery material which can be used for the layers of LEDs, while protectiong proteins from external influences. (reset.org)
  • The protein LEDs, named „OLEDs" (organic LEDs), could thus soon be a cost-effective and organic alternative to the color filters made of rare earth elements. (reset.org)
  • biotech
  • While in Miami, he studied the genetics of pigmentation in corals and other invertebrates, designed and built an aquaculture facility for Caribbean corals, performed well over 500 SCUBA dives, and licensed a spin-off product of his research (a patented fluorescent protein) to a biotech company. (creation.com)
  • We increased the brightness of several of these proteins by manipulating their DNA code and marketed one of them to a biotech company. (creation.com)
  • variants
  • To develop FlicR1, we used directed protein evolution and rational engineering to screen libraries of thousands of variants. (jneurosci.org)
  • transformants
  • The transformants showing high expression of the gfp gene had the normal mycelia pigmentation altered, displaying a bright green-yellowish color, visible with the naked eye on the plates, without the aid of any kind of fluorescent light or special filter set. (ufv.br)
  • neurons
  • Optical imaging of voltage indicators based on green fluorescent proteins (FPs) or archaerhodopsin has emerged as a powerful approach for detecting the activity of many individual neurons with high spatial and temporal resolution. (jneurosci.org)
  • SIGNIFICANCE STATEMENT Fluorescent-protein-based voltage indicators enable imaging of the electrical activity of many genetically targeted neurons with high spatial and temporal resolution. (jneurosci.org)
  • bright
  • Relative to green FP-based voltage indicators, a bright red-shifted FP-based voltage indicator has the intrinsic advantages of lower phototoxicity, lower autofluorescent background, and compatibility with blue-light-excitable channelrhodopsins. (jneurosci.org)
  • Here, we describe the engineering of a bright red fluorescent protein-based voltage indicator designated as FlicR1 (fluorescent indicator for voltage imaging red). (jneurosci.org)
  • Do you have some bright green slime algae? (aquarium-parts.co.uk)
  • glow
  • The original GFP wasn't perfect, however, as it had a tendency to lose its ability to glow, and, for technical reasons, green isn't the most convenient color. (arstechnica.com)
  • While it's sometimes possible to see the fluorescent colours in daylight, filming at night with a blue light makes the biofluorescent animals glow much more distinctly, so that's what Gruber did. (cbc.ca)
  • tumors
  • But tiny "protein-factory" particles could target tumors specifically by manufacturing cancer-killing drugs anywhere within the human body. (nbcnews.com)
  • absorb
  • Because blue is the colour that travels best through water, most biofluorescent marine creatures absorb blue light and emit green, yellow or red. (cbc.ca)
  • yield
  • To optimize DARPins for their application as crystallization chaperones, rigid domain-domain fusions of the DARPins to larger proteins, proven to yield high-resolution crystal structures, were generated. (rcsb.org)