• Remarkably, the GFP molecule folded and was fluorescent at room temperature, without the need for exogenous cofactors specific to the jellyfish. (wikipedia.org)
  • Femtosecond stimulated Raman spectroscopy on GFP involves hitting the protein molecule with an approximately 80 femtosecond pulse of ultraviolet light, which excites many vibrational modes in the molecule, and then a one-two punch of picosecond red and femtosecond white light to stimulate Raman emission. (medgadget.com)
  • These studies demonstrate that introducing GFP as a fusion within the context of a rapidly degraded protein does not alter the degradation properties of the parent molecule, and that the GFP moiety of the fusion protein is degraded along with the rest of the protein. (biomedcentral.com)
  • Another phenotype associated with E. coli strains that produce exported β-galactosidase fusions is overproduction lethality, resulting from high-level synthesis of the hybrid proteins ( 3 , 13 ). (asm.org)
  • Transgenic tobacco BY-2 cell lines expressing these seven sp-GFP-TMD-CT fusions all exhibited typical punctate signals colocalizing with VSR proteins by confocal immunofluorescence. (plantphysiol.org)
  • P. Didier, L. Guidoni, and E. Weiss, "Ultrafast Excited-state Dynamics of Genetic Fusions: The Green Fluorescent Protein as a Folding Reporter," in Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference and Photonic Applications Systems Technologies , Technical Digest (CD) (Optical Society of America, 2006), paper QMH1. (osapublishing.org)
  • We investigated the excited-state ultra-fast dynamics of genetic fusions that link a Green Fluorescent Protein to an antibody fragment. (osapublishing.org)
  • This observation suggests that these proteins can be engineered to provide active control of in vivo processes as optogenetic tools in addition to their conventional roles as passive imaging reporters. (pnas.org)
  • The mutant protein in fluorinated form showed improved folding efficiency both in vivo and in vitro, and the median fluorescence of cells expressing the fluorinated protein was improved {approx}650-fold in comparison to that of cells expressing fluorinated GFPm. (caltech.edu)
  • These proteins may therefore become valuable additions to the in vivo imaging toolkit. (utexas.edu)
  • In vitro assay of degradation is simpler than in vivo analysis, but an in vitro assay system may not fully mimic the degradation of proteins in the cells. (biomedcentral.com)
  • The objective was to establish the value of green-fluorescent protein (GFP) as a nondestructive reporter gene system for characterizing promoter activity at nematode feeding sites in vivo . (apsnet.org)
  • The two proteins colocalize and interact in vivo as well as in vitro and exhibit mutual functional interference. (plantphysiol.org)
  • We have redesigned gfp to reduce H-NS-mediated transcription silencing and simultaneously improve translation in vivo without altering the amino acid sequence of the GFP protein. (tcd.ie)
  • The diversity of genetic mutations is illustrated by this San Diego beach scene drawn with living bacteria expressing 8 different colors of fluorescent proteins (derived from GFP and dsRed). (wikipedia.org)
  • Fitness cost of the green fluorescent protein in gastrointestinal bacteria. (nih.gov)
  • The magnetosomes of magnetotactic bacteria are prokaryotic organelles consisting of a magnetite crystal bounded by a phospholipid bilayer that contains a distinct set of proteins with various functions. (environmental-expert.com)
  • Results We have carried out a screen for mutant forms of GFP that fluoresce more intensely than the wild-type protein when expressed in E. coli at 37°C. We have characterized a bright mutant (GFPA) with reduced sensitivity to temperature in both bacteria and yeast, and have shown that the amino acids substituted in GFPA act by preventing temperature-dependent misfolding of the GFP apoprotein. (deepdyve.com)
  • Fluorescent reference strains of bacteria carrying a stable chromosomally integrated single copy of the gfp gene have been developed. (auckland.ac.nz)
  • In recent years, GFP-like proteins from marine invertebrates have emerged as indispensable tools for tracking of proteins or cells, sensing of intracellular conditions such as Ca 2+ or pH variations and for studies of protein interactions and gene activity, opening several stimulating perspectives in live cell imaging - . (plos.org)
  • Activation of a GPCR results in the modulation of intracellular second messenger levels and/or ionic conductances via the coupling of receptors to a wide variety of effector systems via heterotrimeric guanine nucleotide-binding proteins (G proteins). (scielo.br)
  • By evaluating leader sequence processing and using GFP fluorescence to track intracellular processing, it was determined that the majority of fusion protein synthesized by the yeast was not secreted and in most cases was accumulating in an immature, although active, endoplasmic-reticulum (ER)-processed form. (asm.org)
  • The results indicated that yeast can be used as an effective host for the secretion of scFv/scTCR-GFP fusion proteins and that as a result of intracellular secretory bottlenecks, there is considerable yeast secretory capacity remaining to be exploited. (asm.org)
  • In cardiac precursor cells, I Ca was found to be already under control of cAMP-dependent phosphorylation since intracellular infusion of the catalytic subunit of protein kinase A resulted in a 1.7-fold stimulation. (rupress.org)
  • The ActA protein of Listeria monocytogenes is an essential virulence factor and is required for intracellular bacterial motility and cell-to-cell spread. (asm.org)
  • Comparisons between split GFPs and other photosensory proteins, like photoactive yellow protein and rhodopsin, provide potential strategies for improving the photodissociation quantum yield. (pnas.org)
  • This jamming event appears to be the result of the conformation assumed by β-galactosidase and not of the presence of specific amino acid sequences that are incompatible with the protein export machinery ( 32 , 47 ). (asm.org)
  • PVCs in tobacco ( Nicotiana tabacum ) BY-2 cells are multivesicular bodies (MVBs) as defined by VSR proteins and the BP-80 reporter, where the transmembrane domain (TMD) and cytoplasmic tail (CT) sequences of BP-80 are sufficient and specific for correct targeting of the reporter to PVCs. (plantphysiol.org)
  • Toward this goal, we have generated seven chimeric constructs containing signal peptide (sp) linked to green fluorescent protein (GFP) and TMD/CT sequences (sp-GFP-TMD/CT) of the seven individual AtVSR. (plantphysiol.org)
  • Four chimeric GFPs carrying one and two-consecutive sequences of the Fc-binding motif (Z-domain), derivative of IgG-binding B domain of Staphylococcal protein A (SpA), at the C-terminus were constructed. (tu-dortmund.de)
  • The resulting GFP-S65T mutant was a distinct improvement over wild-type GFP (wtGFP) for applications as a fluorescent marker in living cells because it had a well-defined absorption profile with a single peak at 489 nanometers. (zeiss.com)
  • 21 ] found that cis -to- trans photoisomerization should be a general mechanism of green fluorescent protein chromophores whose efficiency can be modulated by the detailed mutant-specific protein environment. (hindawi.com)
  • Although this near-wtGFP was fluorescent, it had several drawbacks, including dual peaked excitation spectra, pH sensitivity, chloride sensitivity, poor fluorescence quantum yield, poor photostability and poor folding at 37 °C. The first reported crystal structure of a GFP was that of the S65T mutant by the Remington group in Science in 1996. (wikipedia.org)
  • A similar recovery of green fluorescence is also observed for the E222Q/H148D mutant, suggesting that D148 is the proton acceptor for the ESPT reaction in both double mutants. (unboundmedicine.com)
  • Nuclear accumulation of a mutant form of the nuclear protein Lamin-A, called Progerin (PG) or Lamin AΔ50, occurs in Hutchinson-Gilford Progeria Syndrome (HGPS) or Progeria, an accelerated aging disease. (nih.gov)
  • Although this near-wtGFP was fluorescent, it had several drawbacks, including dual peaked excitation spectra, pH sensitivity, chloride sensitivity, poor fluorescence quantum yield, poor photostability and poor folding at 37 °C. (wikipedia.org)
  • Although this wild-type GFP was fluorescent, it had several drawbacks, including dual peaked excitation spectra, poor photostability and poor folding at 37°C. (wikidoc.org)
  • Changes in GFP levels altered the extent of quenching, by this protein, of red light emitted by chlorophyll within the chloroplasts under violet excitation. (apsnet.org)
  • Using a unique and relatively simple cell-based fluorescent assay they developed, scientists with the U.S. Department of Energy's Lawrence Berkeley National Laboratory and the University of California, Berkeley have identified a means by which fluoxetine, the active ingredient in Prozac, suppresses the activity of the TREK1 potassium channel. (news-medical.net)
  • The output of entire protein was found by usage of a Bradford Assay and a standard curve. (bluecrewsportsgrill.com)
  • Here, a neutralization assay based on a modified RSV strain expressing the green fluorescent protein in combination with automated detection and quantification of plaques is described. (biomedcentral.com)
  • Scientists Roger Y. Tsien , Osamu Shimomura , and Martin Chalfie were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein. (wikipedia.org)
  • Until recently, there was no commercial source for plasmids encoding Emerald (it is now available from Invitrogen), so there has been limited use of this high-performance green variant. (zeiss.com)
  • In order to obtain the required protein in a soluble and functional format, IBs usually undergo solubilization and refolding processes, which are expensive, time consuming and highly proteindependent . (scribd.com)
  • The success of this approach demonstrates the feasibility of engineering functional proteins containing many copies of abiological amino acid constituents. (caltech.edu)
  • Wielka Orkiestra w Zespole Szkół Specjalnych This continues the functional download Green to be a safely alternative field at confronting. (sokp.pl)
  • Split GFPs have been widely applied for monitoring protein-protein interactions by expressing GFPs as two or more constituent parts linked to separate proteins that only fluoresce on complementing with one another. (pnas.org)
  • Typically, the protein is expressed as two or more constituent parts linked to separate proteins that only fluoresce on complementing with one another, offering readouts of protein-protein colocalizaton with low background and high specificity ( 5 ). (pnas.org)
  • The AcGFP1 protein is stable, allowing you to monitor fluorescence over extended periods of time. (clontech.com)
  • Split GFPs have been widely applied for imaging as fluorescent reporters of cellular processes, because they are small (∼25 kDa), are stable in cytosol, produce chromophores autocatalytically, and are amenable to mutation and circular permutation ( 4 ). (pnas.org)
  • Now-a-days GFP is creating revolution in the field of science by its applications and properties.GFP is a stable protein extracted from the photo organs of the jellyfish Aequoria victoria by Shimomura et al in 1962. (ukessays.com)
  • Cellular proteins differ widely in their lability, ranging from those that are completely stable to those with half-lives measured in minutes. (biomedcentral.com)
  • Some components of pH homeostasis act in the presence of chloramphenicol, whereas others require ongoing protein synthesis ( 10 ). (asm.org)
  • Individual subpopulations were treated with cycloheximide (CHX) to inhibit protein synthesis and re-sorted after 2 hours of treatment. (biomedcentral.com)