• A FMN-binding fluorescent protein (FbFP), also known as a LOV-based fluorescent protein, is a small, oxygen-independent fluorescent protein that binds flavin mononucleotide (FMN) as a chromophore. (wikipedia.org)
  • The photophysical properties of the FbFPs are determined by the chromophore itself and its chemical surrounding in the protein. (wikipedia.org)
  • Since all GFP derivatives and homologues require molecular oxygen for the maturation of their chromophore, these fluorescent proteins are of limited use under anaerobic or hypoxic conditions. (wikipedia.org)
  • The key to rationally design the next-generation biosensors with improved and targeted properties lies in the mechanistic understanding of molecular fluorescence, emitted from the chromophore that is an organic moiety embedded in the center of the protein pocket. (oregonstate.edu)
  • Nonlinear Optical Properties of Green Fluorescent Protein Chromophore Coupled Diradicals [J]. Acta Phys. (pku.edu.cn)
  • The unique property of being selfsufficient to form a visible wavelength chromophore from a sequence of three amino acids within their own polypeptide sequence distinguishes fluorescent proteins from other structurally homologous proteins. (eresearchco.com)
  • Here, we investigate the photophysical properties associated with the S1 state of three alkylated derivatives of the chromophore in GFP, in the gas phase using time-resolved photoelectron imaging, and in water using femtosecond fluorescence upconversion. (uea.ac.uk)
  • Most of our recombinant proteins are fluorescent derivatives of various DT and proHB-EGF. (org.ua)
  • positive controls may include Western blot data generated against recombinant proteins or lysates from cell lines known to express high levels of the target antigen, whereas negative controls might be microscopy images from plated cells which have been treated with siRNA. (biozol.de)
  • To facilitate the study of these neurons, transgenic mice were generated that express enhanced green fluorescent protein (EGFP) in subpopulations of GABAergic neurons. (jneurosci.org)
  • expression Mammalian Cepko 8389 p212 pCMV-EGFP/RFP EGFP- dsRed gene switch plasmid Mammalian Green 22799 Ai9 Rosa26. (addgene.org)
  • This causes a conformational change in the protein that is necessary for signal propagation and also leads to the loss of fluorescence. (wikipedia.org)
  • In order to make better use of the fluorescence properties of these proteins, the natural photocycle of these LOV-domains was abolished by exchanging the conserved cysteine against an alanine on a genetic level. (wikipedia.org)
  • Thus, upon blue light irradiation, the protein remains in the fluorescent state and also exhibits a brighter fluorescence. (wikipedia.org)
  • His current research focuses on developing state-of-the-art spectroscopic techniques to reveal the fluorescence mechanisms of green fluorescent protein (GFP) derivatives and emerging fluorescent protein biosensors. (oregonstate.edu)
  • Our group will use the newly available resources to systematically elucidate fluorescence mechanisms in an emerging group of protein biosensors, and pinpoint strategic atomic sites that protein designers and engineers can target to rationally improve the properties of those biosensors. (oregonstate.edu)
  • In addition, the lowered emission filter bandpass results in improved blocking of yellow to red wavelengths, with only green fluorescence appearing in images acquired using the B-2E/C filter combination (See Figure 2). (microscopyu.com)
  • Green fluorescence from the nuclei stained with SYTOX Green in a thin section of mouse intestine is illustrated in Figure 2(b). (microscopyu.com)
  • Note the presence of clean signal (low background) from the green fluorophore, but the absence of fluorescence intensity from the blue and red probes. (microscopyu.com)
  • Note the absence of signals from the orange (Alexa Fluor 555) and far-red fluorophores, but the presence of intense green fluorescence from several of the more prominent actin cytoskeletal elements. (microscopyu.com)
  • Fluorescence recovery after photobleaching (FRAP), also known as fluorescence redistribution after photobleaching, is a microscopy-based technique for tracking the movement of fluorescent molecules over time. (fluorofinder.com)
  • Fluorescence localization after photobleaching ( FLAP ) is a useful approach for analyzing protein mobility and dynamics that occur within a short time frame. (fluorofinder.com)
  • The fluorescence intensities of both fluorophores are measured to generate ratiometric measurements that serve as direct indicators of protein movement. (fluorofinder.com)
  • Using fluorescence microscopy, biologists commonly introduce a gene that encodes an engineered fluorescent protein into living cells and then examine the location and dynamics of the gene product. (eresearchco.com)
  • The green fluorescent protein and its colorshifted genetic derivatives have been of great use in many thousands of live-cell imaging experiments thanks to recent technological advancements in wide field fluorescence and confocal microscopy, such as ultrafast low light level digital cameras and multitracking laser control systems. (eresearchco.com)
  • Maturation of DsRed fluorescence occurs slowly and proceeds through a time period when fluorescence emission is in the green region. (luyor.cn)
  • Many fluorescent biosensors exploit conformation-dependent changes in the fluorescence resonance energy transfer (FRET) between two engineered fluorophores that emit at two different colors (wavelength ranges). (nature.com)
  • The specimen was simultaneously labeled with primary anti-human OxPhos Complex V inhibitor protein mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 555 (emission maximum at 571 nanometers). (microscopyu.com)
  • Current, antibody-based diagnostical methods utilize antibodies against histamine raised by conjugating histamine to a large immunogenic protein carrier, such as bovine serum albumin (BSA) or ovalbumin (OVA) via its primary amine group. (justia.com)
  • Consequently, only the imidazole will be exposed to lymphocytes, which commonly results in generation of antibodies that recognize protein-bound histamine with only limited affinity and sensitivity for free histamine. (justia.com)
  • Manufacturer of outstanding antibodies against cardiac proteins and phospho-proteins, and the manufacturer of leading kits for the study of protein S-palmitoylation. (badrilla.com)
  • Sandia Biotech's recombinant fluorescent antibody derivatives, FluorAbodies®, are not derived from animal hosts, they are recombinant (derived from bacteria) intrinsically fluorescent proteins that offer a number of advantages when compared to fluorochrome-labeled antibodies. (sandiabio.com)
  • Within the past 2 years, investigational peptide- and recombinant protein-based serologic assays that can more easily differentiate the antibodies to HTLV-I and HTLV-II have been developed (8,9). (cdc.gov)
  • The inner fluid can contain cargo molecules such as nucleic acids or soluble proteins. (biomedcentral.com)
  • Eye proteins are the biological molecules that make up the various structures of the eye and are essential for its proper function. (lookformedical.com)
  • In addition, the ratio between the mobile and immobile fluorescent molecules can be calculated to infer the properties of the sample, such as its viscosity. (fluorofinder.com)
  • Viral-like nanoparticles (also referred to as virus-like particles (VLPs)) of the present disclosure, generally, are assembled from L1 capsid proteins, or a combination of L1 and L2 capsid proteins, and the photosensitive molecules, in some embodiments, are conjugated to a capsid protein that forms the viral-like nanoparticle. (justia.com)
  • Thus, various aspects of the disclosure provide tumor-targeting viral-like nanoparticles that comprise photosensitive molecules conjugated to capsid proteins. (justia.com)
  • Molecules can be transported around a cell by so-called motor proteins that move along a network of filaments called microtubules. (elifesciences.org)
  • To identify novel antiviral compounds with potent anti-OPXV potential, we characterized the inhibitory activity of PAV-866 and other methylene blue derivatives against the prototypic poxvirus, vaccinia virus (VACV). (cdc.gov)
  • Green fluorescent protein has been engineered to produce a plethora of diversely colored mutants, fusion proteins, and biosensors that are collectively referred to as fluorescent proteins since these initial studies. (eresearchco.com)
  • Fluorescent protein biosensors have unlocked major advances in cell biology, neurobiology, clinical chemistry, environmental monitoring, and agricultural technologies 1 . (nature.com)
  • In the accompanying article 2 , we have uncovered a subtle, but powerful, fluorescent signal transduction mechanism (Figure 1) that we anticipate will enable development of new fluorescent biosensors. (nature.com)
  • Accordingly, fluorescent biosensors are created by coupling distinct protein conformations to changes in the electronic structure of a fluorophore, or pairs of fluorophores that have been engineered into the protein. (nature.com)
  • Consequently, different classes of fluorophores whose response mechanisms directly probe delicate local structural changes could be more generalizable tools to build fluorescent biosensors. (nature.com)
  • As such as proteins, lipids, glycans, nucleic acids, and isoporous ultrafiltration membrane they can apparently combination of that. (2medicalcare.com)
  • Peptide pore blockers and their fluorescent derivatives are useful molecular probes to study the structure and functions of the voltage-gated potassium Kv1.3 channel, which is considered as a pharmacological target in the treatment of autoimmune and neurological disorders. (computablegenomix.com)
  • The second-generation DsRed, known as DsRed2 , contains several mutations at the peptide amino terminus that prevent formation of protein aggregates and reduce toxicity. (luyor.cn)
  • Furthermore, the fixed spectral characteristics of interference filters do not allow optimization for different fluorophore combinations, nor for adaptation to new fluorescent dyes, limiting the versatility of both the excitation and detection functions of the microscope. (olympusconfocal.com)
  • The emission bandpass region is somewhat lower in wavelength than that of the B-1E and B-2E filter sets to provide optimum detection of popular fluorochromes such as FITC, BODIPY, Fluo-3, oxacarbocyanine dyes ( DiO ), and green fluorescent protein ( GFP ). (microscopyu.com)
  • FRAP requires fluorescently labeling a target of interest by either expressing it as a fusion protein or tagging it with reactive ligands that bind to fluorescent dyes. (fluorofinder.com)
  • Selecting fluorophores resistant to photobleaching - Green Fluorescent Protein (GFP) and its derivatives are often used as fusion proteins, and the Alexa Fluor® and DyLight™ dyes are also popular for FRAP. (fluorofinder.com)
  • Unique properties, such as red-shifted emission above 600 nm or photo conversions from a greenemitting state to a red-emitting state, can be displayed by fluorescent proteins with other chromophores, such as UnaG with bilirubin. (eresearchco.com)
  • Our recombinant nontoxic toxin analogues and derivatives were applied in studying of toxin interaction with cell surface, intracellular transport of DT and in determination of proHB-EGF expression level. (org.ua)
  • The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. (lookformedical.com)
  • Inverse FRAP ( iFRAP ) tracks the movement of fluorescent proteins out of intracellular components by photobleaching the area surrounding the region of interest. (fluorofinder.com)
  • High-affinity rabbit polyclonal monoclonal antibody to GFP: IgG affinity purified on GFP protein. (badrilla.com)
  • The company is specializing in novel proprietary antibody and protein based reagents for use by biomedical research scientists. (sandiabio.com)
  • FluorAbodies® - In November 2015, Sandia Biotech licensed a patented, recombinant fluorescent antibody platform from STC.UNM, the technology transfer organization of the University of New Mexico and Queen Mary University London to develop a novel panel of recombinant intrinsically fluorescent antibody derivatives. (sandiabio.com)
  • At the heart of any fluorescent signal transduction mechanism is the phenomenon of conformational coupling which Jacques Monod referred to as the "secret of life", because it constitutes the molecular basis of most biological processes such as enzyme control, ligand transport across membrane, motor proteins, or cellular signal transduction 3 . (nature.com)
  • Motor proteins of the conserved kinesin-14 family have important roles in mitotic spindle organization and chromosome segregation. (elifesciences.org)
  • In this study, we show that the yeast kinesin-14 Kar3 generates processive movement as a heterodimer with the non-motor proteins Cik1 or Vik1. (elifesciences.org)
  • Many motor proteins-including the kinesin family of these proteins-can only move in one direction along a microtubule. (elifesciences.org)
  • Although FRAP was initially used to monitor the lateral diffusion of proteins in cell membranes, this technique is now used to analyze other membrane-associated proteins involved in cell adhesion, migration, endocytosis, and signal transduction. (fluorofinder.com)
  • Binding of a small molecule to the sensor (e.g. glucose or neurotransmitters), interactions of a sensor with another protein (e.g. cellular signal transduction networks), or changes in the physical environment of the sensor such as a strong electrical field (e.g. detection of electrochemical pulses along a neuron) are typical chemical events. (nature.com)
  • right panel shows high magnification TEM through a JO sensory cilium, pseudocolored to show the ciliary membrane (peach), microtubule doublets (purple) inner dynein arms (yellow), outer dynein arms (green) and an accessory microtubule derivative (navy). (uiowa.edu)
  • this raises questions about how the Kar3 motor protein moves along the microtubule, and whether this affects the roles the motor performs. (elifesciences.org)
  • It has a signal sequence-like N-terminal segment that, according to studies with green and red fluorescent protein chimeras, anchors unassembled PulG in the inner membrane. (pasteur.fr)
  • We analyzed the early steps of PulG inner membrane targeting and insertion in Escherichia coli derivatives defective in different protein targeting and export factors. (pasteur.fr)
  • The cystovirus Pseudomonas phage phi6 has an envelope that harbors five viral membrane proteins and phospholipids derived from the cytoplasmic membrane of its Gram-negative host. (biomedcentral.com)
  • Our results demonstrate that the phi6 major envelope protein P9 can trigger formation of cytoplasmic membrane structures in E. coli in the absence of any other viral protein. (biomedcentral.com)
  • The bilayer is composed of phospholipids and is typically embedded with membrane proteins. (biomedcentral.com)
  • Regulatory proteins that down-regulate phosphorylated G-protein membrane receptors, including rod and cone photoreceptors and adrenergic receptors. (lookformedical.com)
  • Create separate constructs for the membrane and envelope structural proteins that can be added to cultured cells for packaging the replication-defective virus. (promegaconnections.com)
  • For the 30kb DNA version of SARS-CoV-2, these structural elements are the envelope and membrane proteins. (promegaconnections.com)
  • Without the membrane and envelope proteins that make up most of the viral particle outer layer, the virus is incapable of infecting other cells. (promegaconnections.com)
  • Based on these S-layer proteins, supramolecular assemblies can be constructed which are envisaged for label-free detection systems, as affinity matrix, as anti-allergic immuno-therapeutics, as membrane protein-based screening devices, and as drug targeting and delivery systems. (2medicalcare.com)
  • Previously, we have designed genetic constructs that encode various recombinant derivatives of DT and proHB-EGF structural parts and domains. (org.ua)
  • In addition, FRAP is gaining popularity as a technique for investigating protein dynamics within cells. (fluorofinder.com)
  • The ongoing development of fluorescent proteins require a detailed understanding of the photophysics of the biochromophore, and how chemical derivatisation influences the excited state dynamics. (uea.ac.uk)
  • Results: In E. coli, most of the changes occurred on proteins and lipids, suggesting a key effect on lipopolysaccharides in the first irradiation times. (ua.pt)
  • Although it is highly homologous to G-PROTEIN-COUPLED RECEPTOR KINASE 2, it is not considered to play an essential role in regulating myocardial contractile response. (lookformedical.com)
  • Although the gene for green fluorescent protein was first cloned in 1992, it wasn't until several years later that fusion products were used to track gene expression in bacteria and nematodes that the gene's significant potential as a molecular probe was realized. (eresearchco.com)
  • In hindsight, the first demonstrations of recombinant expression in organisms other than jellyfish and the cloning of the gene represent a clearly discernible turning point in the history of fluorescent protein research. (eresearchco.com)
  • Because the construction of red-shifted mutants from the Aequorea victoria jellyfish green fluorescent protein beyond the yellow spectral region has proven largely unsuccessful, investigators have turned their search to the tropical reef corals. (luyor.cn)
  • In contrast to the jellyfish fluorescent proteins, which have been successfully used to tag hundreds of proteins, DsRed conjugates have proven much less successful and are often toxic. (luyor.cn)
  • A G-protein-coupled receptor kinase subtype that is primarily expressed in the MYOCARDIUM and may play a role in the regulation of cardiac functions. (lookformedical.com)
  • This study evaluated the minimum requirements for the formation of phi6-specific vesicles and the possibility to localize P9-tagged heterologous proteins into such structures in Escherichia coli . (biomedcentral.com)
  • Another advantage to building the SARS-CoV-2 from the ground up is that the codons used for synthesizing the needed viral proteins can be optimized for mammalian cells. (promegaconnections.com)
  • Fluorescent labeling of proteins by fluorophore Prodan is a useful tool to measure protein conformational changes via color switching, however, structure-based rules that predict where to emplace fluorophores that elicit robust fluorescent responses are challenging to establish. (nature.com)
  • A family of naphthalene-based fluorophores, the Prodan family, was developed in the early 1970s by Gregorio Weber's research group who argued that pairing an electron donor group (amine) with an acceptor (carbonyl) on either end of a two-membered aromatic ring system would result in a fluorescent excited state that is particularly sensitive to electrostatic environment of the fluorophore 4 . (nature.com)
  • The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport. (lookformedical.com)
  • Biosensor development depends on a deep understanding of molecular mechanisms that transduce the chemical reactions (events) into a fluorescent signal, which can be challenging to establish. (nature.com)
  • The biological research community was quick to recognize the unique value of a genetically encoded fluorophore as a marker of gene expression and protein localization, armed with the arsenal of potent molecular biology techniques developed over the previous two decades. (eresearchco.com)
  • By allowing researchers to use molecular cloning techniques to fuse the fluorophore moiety to a wide variety of protein and enzyme targets in order to monitor cellular processes in living systems using optical microscopy and related methods, the early 1960s discovery of green fluorescent protein ultimately heralded a new era in cell biology. (eresearchco.com)
  • In the planar form, the corresponding fluorescent emission is green, whereas the twisted fluorophore emits with a blue color. (nature.com)
  • The apo- and glucose-bound protein conformations are coupled to different fluorophore twists, creating a two-color glucose sensor. (nature.com)
  • Even though the FRET distance dependence is steep (the sixth power of inter-fluorophore separation), it is challenging to engineer FRET sensors with large signal changes if the protein conformational changes of interest are small or confined to small regions within the protein, as is the case for many conformational coupling events. (nature.com)
  • Study on Photophysical Behaviors of Styryl Pyrazine Derivatives[J]. Acta Phys. (pku.edu.cn)
  • found that the Kar3-Cik1 motor binds to and transports a protein complex that is crucial for separating chromosomes during cell division. (elifesciences.org)
  • Optical Properties and Responsive Mechanism of Carbazole-Based Two-Photon Fluorescent Probes for the Detection of Hypochlorite [J]. Acta Phys. (pku.edu.cn)
  • All our DT derivatives were designed to be non-toxic for sensitive cells, so all biosafety requirements were met for safety work of laboratory personnel and protection of surrounding environment. (org.ua)
  • Peripheral blood mononuclear cells from CTL-suspected patients were stimulated with HBHA, early secreted antigenic target 6 (ESAT-6), or purified protein derivative (PPD) for 24 h in the presence of IL-7. (bvsalud.org)
  • The challenge is to develop a derivative of SARS-CoV-2 that reflects how it behaves in the cell but is compromised such that it is unable to infect cells more than a single time. (promegaconnections.com)
  • Remove the coding sequences for structural proteins responsible for generating a virus that can infect more cells. (promegaconnections.com)
  • However, other applications of FRAP have since been identified, including its use for studying the dynamic behavior of proteins inside cells. (fluorofinder.com)
  • Furthermore, DsRed is an obligate tetramer and can form large protein aggregates in living cells. (luyor.cn)
  • A P9-GFP fusion protein was used to study the targeting of heterologous proteins into P9 vesicles. (biomedcentral.com)
  • Production of the GFP-tagged P9 vesicles required P12, which protected the fusion protein against proteolytic cleavage. (biomedcentral.com)
  • FbFPs absorb blue light and emit light in the cyan-green spectral range. (wikipedia.org)
  • Especially the brightness but also the photostability of the proteins were enhanced and their spectral characteristics altered. (wikipedia.org)
  • Termed the green state , this artifact has proven problematic for multiple labeling experiments with other green fluorescent proteins because of the spectral overlap. (luyor.cn)
  • Surface (S)-layer proteins and S-layer fusion proteins incorporating functional sequences, self-assemble into monomolecular lattices on solid supports and on various lipid structures. (2medicalcare.com)
  • This leads to an almost complete arrest of protein synthesis in the cell and its death. (org.ua)
  • They disperse primarily through coughing, singing, and other forced respiratory maneuvers by people who have active pulmonary or laryngeal TB and whose sputum contains a large number of organisms (about 10,000 organisms/mL, the limit of detection by fluorescent microscopy). (msdmanuals.com)
  • Isolated vesicles contained predominantly P9-GFP, suggesting selective incorporation of P9-tagged fusion proteins into the vesicles. (biomedcentral.com)
  • The possibility to locate heterologous proteins into the P9-lipid vesicles facilitates the production of vesicular structures with novel properties. (biomedcentral.com)
  • Martin Chalfie of the Department of Biological Sciences, Columbia University, won the 2008 Nobel Prize in Chemistry for the discovery and development of the green fluorescent protein, GFP. (amrita.edu)
  • GFP derivatives, but are instead related to the dsRed protein isolated from Discosoma sp. (addgene.org)
  • The first coral-derived fluorescent protein to be extensively utilized was derived from Discosoma striata and is commonly referred to as DsRed . (luyor.cn)
  • Cyan fluorescent protein, or CFP, is the latter. (eresearchco.com)
  • These tools are now available in three colors a(cyan, green, or yellow) and are marketed to the academic, biotechnology, and pharmaceutical research communities throughout the world. (sandiabio.com)
  • The difference to free FMN is even more significant in the case of the photostabaility, the proteins resistance to bleach out during prolonged and intense irradiation with blue light. (wikipedia.org)
  • This fluorescent protein has a maximum emission wavelength of 450 nm. (eresearchco.com)
  • Nuclei were stained with the far-red fluorescent probe TO-PRO-3 (emission maximum at 661 nanometers). (microscopyu.com)
  • A fluorescent protein with an emission maximum at 500 nm is produced when tryptamine replaces tyrosine. (eresearchco.com)
  • They can have enough distance between their excitation and emission wavelengths to convert red to green light. (eresearchco.com)
  • Replace the structural proteins in the DNA version of the viral genome with reporter genes. (promegaconnections.com)
  • Additional studies are in progress to determine the in vivo efficacy of these compounds against OPXVs and further characterize the anti-viral effects of these derivatives. (cdc.gov)
  • The phi6 major envelope protein P9 and the non-structural protein P12 are essential for the envelopment of its virions. (biomedcentral.com)
  • The only bacteriophages known to have a lipid envelope around their protein capsids are the members of the Cystoviridae family [ 6 ]. (biomedcentral.com)
  • Early studies on nonsense mutants of phage phi6 suggested that the major envelope protein P9 and the non-structural protein P12 are the only proteins needed for phi6 virion envelopment [ 23 ]. (biomedcentral.com)
  • The traditional triad in the medical diagnosis of multiple myeloma may be the recognition of M proteins in the serum or urine (Bence-Jones proteins), higher than 10% plasmacytosis in the bone tissue marrow, and the current presence of osteolytic lesions by radiography. (siamtech.net)
  • A family of serine-threonine kinases that are specific for G-PROTEIN-COUPLED RECEPTORS. (lookformedical.com)
  • A ubiquitously expressed G-protein-coupled receptor kinase subtype that has specificity for the agonist-occupied form of BETA-ADRENERGIC RECEPTORS and a variety of other G-PROTEIN-COUPLED RECEPTORS. (lookformedical.com)
  • A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. (lookformedical.com)
  • G Protein Estrogen Receptors- Sandia Biotech offers agonists and antagonists for the G-protein estrogen receptor (GPER) signaling pathway that is important in a number of cancers. (sandiabio.com)
  • Next-generation red and green G-protein-coupled receptor-based dopamine sensors with improved properties have been developed. (nature.com)
  • Properties of substituents have great influence on the photophysical and photochendcal behavior of the synthesized pyrazine derivatives. (pku.edu.cn)
  • A major goal of fluorescent protein development has become the construction of a red-emitting derivative that equals or exceeds the advanced properties of enhanced green fluorescent protein. (luyor.cn)
  • A 48-Kd protein of the outer segment of the retinal rods and a component of the phototransduction cascade. (lookformedical.com)
  • Several isoforms of the protein with molecular sizes of 47 kDa and 52 kDa exist due to multiple ALTERNATIVE SPLICING. (lookformedical.com)
  • Nucleoid-associated proteins (NAPs) regulate multiple cellular processes such as gene expression, virulence, and dormancy throughout bacterial species. (bvsalud.org)
  • On the other hand, DT represents a very interesting molecular machine which allows using this protein in different bioengineering applications, such as ablation of certain cell types in transgenic mice, development of immunotoxins targeted for specific cell markers and also for delivery of certain proteins into the cytosol. (org.ua)
  • The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS). (lookformedical.com)
  • Selection of laboratory and/or imaging studies to rule out conditions other than migraine headache is determined by the individual presentation (eg, erythrocyte sedimentation rate and C-reactive protein levels may be appropriate to exclude temporal/giant cell arteritis). (medscape.com)
  • They are available on our website as protein, detection and analysis tools and also through our distributors, (MilliporeSigma, Funakoshi, Interchim, Protein Technologies). (sandiabio.com)
  • Among the advantages of a suitable red fluorescent protein are the potential compatibility with existing confocal and widefield microscopes (and their filter sets), along with an increased capacity to image entire animals, which are significantly more transparent to red light. (luyor.cn)
  • A conformationally sensitive fluorescent color-switching mechanism. (nature.com)
  • In conformational coupling, ligand binding or perturbations of the physical environment are associated with changes in the distribution of protein conformational states. (nature.com)
  • In S. warneri, proteins were the major molecular targets of oxidative damage but phospholipids and polysaccharides were also affected. (ua.pt)