Granulocyte macrophage progenitor cells. Medical search. Frequent questions

The innate immune system is mainly composed of myeloid lineage cells, such as macrophages, neutrophils, and mast cells [ 2 ]. (hindawi.com)
Although tumor cells were first thought to drive the cellular events underpinning tumor angiogenesis and growth, considerable evidence has now emerged for the central role of tumor infiltrating myeloid cells such as monocytes, macrophages, and neutrophils in this phenomenon [ 8 - 12 ]. (hindawi.com)
IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. (biolegend.com)
These cells are closely related to neutrophils and monocytes. (aacrjournals.org)
Granulocytic myeloid cells include populations of terminally differentiated polymorphonuclear neutrophils, eosinophils, basophils, and mast cells. (aacrjournals.org)
Myeloid cells generated under these conditions, although similar to neutrophils and monocytes in morphology and phenotype, have different genomic and biochemical profiles and functional activity. (aacrjournals.org)
The field started changing in the late 1990s when the Gr1 + CD11b + cellular phenotype was suggested as defining the immune-suppressive myeloid cells in spleens of mice and when these cells were shown to be phenotypically similar but functionally distinct from monocytes and neutrophils ( 2, 3 ). (aacrjournals.org)
Eosinophils are granulocytes (white blood cells that contain granules in their cytoplasm) derived from the same progenitor cells as monocytes-macrophages, neutrophils, and basophils. (msdmanuals.com)
This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils, and macrophages. (biolegend.com)
This decreased the total number and composition of microbes in the intestine of the neonates and was associated with decreased numbers of circulating and bone marrow neutrophils and granulocyte/macrophage-restricted progenitor cells in the bone marrow of antibiotic-treated and germ-free neonates, resulting in impaired host defense and increased susceptibility to Escherichia coli K1 and Klebsiella pneumoniae sepsis in antibiotic-treated neonates. (gutmicrobiotaforhealth.com)

It serves as an in vitro model for MONOCYTE and MACROPHAGE differentiation. (umassmed.edu)
The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
Additionally, edited CD34 + cells can be further cultured in conditions stimulating lineage-specific differentiation by combining StemSpan™ media with expansion supplements designed for the expansion and differentiation of progenitors to erythroid, myeloid, megakaryocyte, natural killer (NK), or T cell lineages. (stemcell.com)
Huang and Chang then looked at the proteins GATA1 and PU.1, transcription factors that normally favor differentiation into red and white blood cells, respectively. (sciencedaily.com)
Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
Retinoic acid modulates IL-5 receptor expression and selectively inhibits eosinophil-basophil differentiation of hemopoietic progenitor cells. (dadamo.com)
Similarly, ATRA (10(-6) mol/L) inhibited eosinophil-basophil differentiation of cord blood CD34(+) cells in liquid culture, whereas neutrophil differentiation proceeded without impediment. (dadamo.com)
CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage. (thermofisher.com)
CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. (thermofisher.com)
Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. (thermofisher.com)
The breast cancer stem cells have the ability of infinite proliferation and multi-directional differentiation by themselves. (omicsonline.org)
The proliferation and differentiation of pluripotent stem cells give rise to progeny that can populate the entire immunologic and hematopoietic systems through committed progenitors of both the lymphoid and myeloid lineages. (medscape.com)
GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells. (biolegend.com)
In this study, we investigated how higher-order chromatin structure modulates differential expression of the human INK4b-ARF-INK4a locus during progenitor cell differentiation, cellular ageing and senescence of cancer cells. (biomedcentral.com)
We found that INK4b and INK4a , but not ARF , are upregulated following the differentiation of haematopoietic progenitor cells, in ageing fibroblasts and in senescing malignant rhabdoid tumour cells. (biomedcentral.com)
During progenitor cell differentiation and ageing, PcG silencer EZH2 attenuates, causing loss of PRC binding and transcriptional activation of INK4b and INK4a . (biomedcentral.com)
Developmentally regulated EZH2 levels are one of the factors that can determine the higher order chromatin structure and expression pattern of the INK4b-ARF-INK4a locus, coupling human progenitor cell differentiation to proliferation control. (biomedcentral.com)
Development and homeostasis require the coordinate regulation of cell proliferation and differentiation. (biomedcentral.com)

Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
Since its discovery in 2000, initial in vitro studies have found that the engagement of Siglec-8 with a monoclonal antibody or with selective polyvalent sialoglycan ligands induced the cell death of eosinophils and inhibited mast cell degranulation. (mdpi.com)
Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
On the other hand, the increase in number of eosinophils and the activation of these cells, both of which are related to elevated IL5 production, are the cause of severe pathologic disorders, as in asthma or hypereosinophilic syndromes. (dadamo.com)

The microenvironment, consisting of lymphocytes, macrophages, fibroblasts, endothelial elements, and stroma in which hematopoietic cells reside, creates a regulatory niche that determines the local area network. (medscape.com)
Different signaling mechanisms regulating IL-6 expression by LPS between gingival fibroblasts and mononuclear cells: seeking the common target. (musc.edu)
GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. (biolegend.com)
To investigate the cytotoxicity of four endodontic sealers with different bases - Epiphany (EPH), AH Plus (AHP), Sealer 26 (S26) and Endofill (ENF) - on human foreskin fibroblasts (HFF) and mouse macrophages (J774/G8). (bvsalud.org)
In order to take a new approach to previous in vitro studies on the cytotoxicity of endodontic materials, it would be interesting to evaluate the response of fibroblasts and macrophages, which are cell lines involved in periapical inflammation and repair. (bvsalud.org)

They began with five distinct, normal cell types found in the bone marrow in both mice and humans: long-term hematopoietic stem cells (HSCs), short-term HSCs, multipotent progenitors, common myeloid progenitors and granulocyte macrophage progenitors. (news-medical.net)

Monocytes and their derivatives, including macrophages and dendritic cells, play diverse roles in the response to fungal pathogens. (frontiersin.org)
Upon entering affected tissues, classical monocytes can further differentiate into macrophages and monocyte-derived dendritic cells (MoDCs). (frontiersin.org)
MoDCs are also distinct in origin from conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) that are derived from a common dendritic cell progenitor (CDP). (frontiersin.org)
Classical "inflammatory" monocytes can give rise to non-classical "patrolling" monocytes or further differentiate into macrophages or monocyte-derived dendritic cells (MoDCs). (frontiersin.org)
Multicolor flow cytometric analysis of mouse splenic dendritic cells. (bdbiosciences.com)
dashed line histograms) or BD Horizon BB515 Rat Anti-Mouse Dendritic Cells antibody (Cat. (bdbiosciences.com)
The fluorescence histogram showing Dendritic Cells antigen expression (or Ig Isotype control staining) were derived from either CD11b+ CD11c- (Left Panel) or CD11b [intermediate] CD11c+ (Right Panel) gated events, as indicated, with the forward and side light-scatter characteristics of intact leucocytes. (bdbiosciences.com)
Mononuclear myeloid cells include terminally differentiated macrophages and dendritic cells (DC), as well as monocytes, which under inflammatory conditions differentiate in tissues to macrophages and DCs. (aacrjournals.org)

Although a difference was found in the radiation survival of control (untreated) day-7 progenitor cells (Do = 1.39 Gy) and day-14 progenitor cells (Do = 0.89 Gy), a similar degree of in vitro radiosensitization was found for BUdR-treated bone marrow progenitor cells scored at day 7 and day 14. (tmu.edu.tw)

The effect of recombinant human granulocyte-macrophage colony stimulating factor on chemotherapy-induced myelosuppression. (wikipedia.org)

The findings, published in the May 22 issue of Nature, give a glimpse into how that collective behavior works, and show that cell populations maintain a built-in variability that nature can harness for change under the right conditions. (sciencedaily.com)
They began by examining populations of seemingly identical blood stem cells, and found that a cell marker of "stemness," a protein called Sca-1, was actually present in highly variable amounts from cell to cell -- in fact, they found a 1,000-fold range. (sciencedaily.com)
Enriched populations of CD34(+) cells were isolated by means of positive immunomagnetic selection with MACS beads. (dadamo.com)
Besides housing hematopoietic stem cells and their progeny, the endosteum and the central marrow also contain distinct cell populations that express molecules or secrete proteins, such as cytokines that regulate the formation, development, and function of HSCs and progenitor cells. (medicalnewstoday.com)
Myeloid cell populations isolated from colonic inflammatory bowel disease (IBD) or murine dextran sulphate sodium (DSS) induced colitis were assessed using flow cytometry and compared to healthy controls. (frontiersin.org)
To determine whether viral effect(s) occurred in adherent stromal and/or non-adherent hematopoietic stem cells, purified cell populations, comprised exclusively of cells from each compartment, were separated from C57BL/6J (Fv-1 bb ) or C3H/HeJ (Fv-1 nn ) marrow cultures and were left uninfected or were infected with host range replication restricted B-tropic or N-tropic helper pseudotype viruses of SFFV respectively. (johnshopkins.edu)
Cell populations were then mixed to establish continuous hematopoiesis in allogeneic or syngeneic combinations. (johnshopkins.edu)
The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations. (biolegend.com)

These cells were therefore described as bone marrow derived endothelial progenitor cells (EPCs). (hindawi.com)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)

All of these fungi can cause invasive disease in a wide-spectrum of immunocompromised patients, such as those with genetic immunodeficiencies, HIV/AIDS, cancer, solid organ, and hematopoietic stem cell transplantation, autoimmune diseases, immunosuppressive treatments, and other predisposing states like diabetes and pregnancy. (frontiersin.org)
Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
Together, both IST and stem cell transplantation are reasonable treatment options for patients with AA. (springermedizin.at)
In order to assess the incidence and analyze reasons which cause prolongation of hospital stay in patients engrafted after peripheral blood stem cell transplantation (PBSCT), we performed this retrospective analysis. (nature.com)
Peripheral blood stem cell and bone marrow transplantation for solid tumors and lymphomas: hematologic recovery and costs. (nature.com)
Allogeneic hematopoietic stem cell transplantation is curative but it requires a histocompatible donor and is associated with significant morbidity and mortality, so it is reserved for severe cases of PNH with aplastic anemia or transformation to leukemia. (medscape.com)

We show that the monocyte:macrophage balance is disrupted in colon inflammation to favour recruitment of CD14 + HLA-DR Int cells in humans, and Ly6C Hi monocytes in mice. (frontiersin.org)

The bone marrow niche includes cells belonging to the stroma, which includes mesenchymal stromal cells , adipocytes (fat cells), nerve cells, connective tissue cells, and cells involved in bone formation and remodeling. (medicalnewstoday.com)
Mesenchymal stromal cells can divide and differentiate into a number of cell types, including bone, cartilage, and adipocytes. (medicalnewstoday.com)
The present study found that aged mice showed a decrease in the population of mesenchymal stromal cells and osteoprogenitor cells that are involved in bone formation in the endosteum. (medicalnewstoday.com)
In contrast, there was an increase in the number of mesenchymal stromal cells in the central marrow of aged mice. (medicalnewstoday.com)
Virus host range restriction between compartments was maintained in allogeneic reconstituted cultures which showed active hematopoiesis for 16-17 weeks and no significant differences between: (1) virus infected and uninfected groups or (2) groups comprised of stromal cells from one or the other strain. (johnshopkins.edu)
Progenitor cell (stem cell) lines in the bone marrow produce new blood cells and stromal cells. (medscape.com)
One type of stem cell is involved in producing blood cells and the other is involved in producing stromal cells, which are responsible for the supporting stroma. (medscape.com)

CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
The findings also help explain why the process of differentiating stem cells into specific lineages in the laboratory has been highly inefficient. (sciencedaily.com)
Currently, little is known concerning regulation of IL-5Ralpha expression in the context of commitment of hemopoietic progenitor cells to the eosinophil and basophil lineages. (dadamo.com)

In normal bone marrow cultures grown in the presence of either granulocyte-macrophage colony-stimulating factor (GM-CSF) or interleukin-3 (IL-3), IL-4 suppresses granulocyte-macrophage colony-forming unit (CFU-GM) proliferation but it enhances the colony-stimulatory effect of granulocyte colony-stimulating factor (G-CSF). (utmb.edu)

Antman developed standards for the treatment of patients receiving chemotherapy including pharmacology, growth factors and mobilization of peripheral blood derived stem cells for blood and marrow transplant. (wikipedia.org)
13 ] isolated mononuclear cells from human peripheral blood that were enriched for expression of the hematopoietic stem cell marker CD34 [ 13 ]. (hindawi.com)
These results suggest that the exposure to environmental pollutants in utero affects genetic constitution of fetus and increases MN values in their body cells, which is detectable right after birth in peripheral blood lymphocytes. (go.jp)
CD184 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. (thermofisher.com)
Applications Tested: This 12G5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
We collected large numbers of peripheral blood (PB) progenitors after CSF administration using either G- CSF or GM-CSF and tested their ability to affect hematopoietic reconstitution and resource utilization in patients undergoing high-dose chemotherapy and autologous BM support. (johnshopkins.edu)
or BM with G-CSF or GM-CSF and G-CSF or GM-CSF primed peripheral blood progenitor cells (PBPC). (johnshopkins.edu)
We studied the effect of IL-4 on chronic myelogenous leukemia (CML) bone marrow or peripheral blood cells from 30 patients using the CFU-granulocyte-erythrocyte-monocyte-megakaryocyte colony culture assay. (utmb.edu)
Incubation of CML low-density peripheral blood cells with IL-4 resulted in down-regulation of IL-1β and IL-6 production in three of four samples, suggesting that the suppressive effect of IL-4 is mediated by inhibition of IL-1 and by other mechanisms including inhibition of IL-6 production. (utmb.edu)

White blood cell, eosinophil, CD4- and CD8-positive lymphocyte counts, and serum interleukin-5 (IL-5) and IgE levels were determined before, 2 and 4 weeks after switching to vitamin E-bonded dialyzers. (dadamo.com)
RESULTS: In semisolid methylcellulose cultures of normal human bone marrow, ATRA (10(-6) mol/L) selectively suppressed eosinophil-basophil colony-forming units but had no effect on granulocyte-macrophage colony-forming units. (dadamo.com)

In vivo incorporation into human bone marrow was assayed by differential chromatid staining as well as by comparison of in vitro radiation survival curves of granulocyte-macrophage progenitor cells scored at both day 7 and day 14. (tmu.edu.tw)
BUdR treatment produced transient moderate myelosuppression that probably resulted from BUdR incorporation into normal marrow cells. (tmu.edu.tw)
Within 1 wk of liquid culture in granulocyte/macrophage colony-stimulating factor (GM-CSF), normal B10 BR (H-2k I-E+) mouse liver nonparenchymal cells (NPC) formed loosely adherent myeloid cell clusters that have been shown to contain dendritic cell (DC) progenitors in similar studies of mouse blood or bone marrow. (silverchair.com)
DMBA reduced bone marrow cells in mice fed control diet to a significantly greater extent than mice fed the SBR diet. (nih.gov)
Myeloid cells are a heterogeneous population of bone marrow-derived cells that play a critical role during growth and metastasis of malignant tumors. (hindawi.com)
Macrophages that differentiate from monocytes in the adult bone marrow are distinguished from tissue resident macrophages (e.g., alveolar macrophages, glial cells) that originally derive from fetal yolk sac progenitor cells or monocytes from the fetal liver ( Hoeffel and Ginhoux, 2018 ). (frontiersin.org)
However, there is evidence that bone marrow-derived monocytes can help replenish the tissue-resident macrophages of specific organs including the gut, the skin, and the heart ( Ginhoux and Guilliams, 2016 ). (frontiersin.org)
NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
Catabolism of 3'-azido-3'-deoxythymidine in hepatocytes and liver microsomes, with evidence of formation of 3'-amino-3'-deoxythymidine, a highly toxic catabolite for human bone marrow cells. (aspetjournals.org)
METHODS: Progenitor cells were obtained from bone marrow aspirates and cord blood samples. (dadamo.com)
When blood-forming hematopoietic stem cells that reside in the bone marrow age, the production of blood cells is altered, and the body's ability to regenerate lost cells is impaired. (medicalnewstoday.com)
A recent study in mice shows that aging is linked to the inflammation of cells in the bone marrow. (medicalnewstoday.com)
The bone marrow is the main site for hematopoiesis , the process of new blood cells forming via the hematopoietic stem cells (HSC) dividing and differentiating. (medicalnewstoday.com)
All these cells, along with blood vessels and the extracellular matrix, form the bone marrow niche . (medicalnewstoday.com)
The endothelial cells that form the lining of these blood vessels are also a major part of the bone marrow niche. (medicalnewstoday.com)
The study found that bone marrow niche cells that support the functioning of HSCs played a key role in blood aging. (medicalnewstoday.com)
In the present study, the researchers performed several analysis in the hindlimb, forelimb, and pelvic bones from young and old mice to examine the impact of aging on the interaction between the bone marrow niche and hematopoietic cells. (medicalnewstoday.com)
Granulocyte-macrophage colony forming units were the most heat resistant bone marrow progenitors tested. (elsevierpure.com)
The bone marrow cell suspensions were then heated in vitro and plated for colony formation. (elsevierpure.com)
Mivechi, NF & Li, GC 1986, ' Lack of Development of Thermotolerance in Early Progenitors of Murine Bone Marrow Cells ', Cancer Research , vol. 46, no. 1, pp. 198-202. (elsevierpure.com)
Replication of the Friend spleen focus forming virus (SFFV) in C3H/HeJ or C57BL/6J mouse continuous bone marrow cultures is associated with an increased cumulative production of pluripotential/hematopoietic stem cells (CFUs), granulocyte-macrophage progenitor cells (GM-CFUc), and total granulocytes, compared to uninfected or helper virus infected control cultures. (johnshopkins.edu)
Thus, these data indicate that the prolongation of hematopoiesis in undisturbed long-term marrow cultures by SFFV occurs through an interaction of adherent hematopoietic stem cells with the marrow stroma. (johnshopkins.edu)
Red blood cell (RBC) production (erythropoiesis) takes place in the bone marrow under the control of the hormone erythropoietin (EPO). (msdmanuals.com)
Myeloproliferative neoplasms are clonal proliferations of bone marrow stem cells, which can manifest as an increased number of platelets, red blood cells (RBCs), or white blood cells (WBCs), alone or in combination, in the circulation and sometimes with fibrosis in the bone marrow and extramedullary hematopoiesis (cell production outside the marrow). (msdmanuals.com)
The etiology of bone marrow failure (BMF) includes defective stem/progenitor cells and/or stroma/accessory cells/growth factors, as well as deficient nonspecific nutrients or, as in the case of acquired aplastic anemia, immune-mediated abnormalities. (medscape.com)
Inherited bone marrow failure syndromes (IBMFS) are usually the result of intrinsic stem cell/progenitor defects. (medscape.com)
Two hematopoietic colony-stimulating factors, granulocyte colony- stimulating factor (G-CSF) and granulocyte-macrophage CSF (GM-CSF), have been shown to accelerate leukocyte and neutrophil recovery after high-dose chemotherapy and autologous bone marrow (BM) support. (johnshopkins.edu)
In contrast to the stimulatory effect exerted by IL-4 on G-CSF-dependent CFU-GM progenitor proliferation in normal marrow, the addition of IL-4 to CML cultures grown in the presence of G-CSF resulted in a divergent effect: suppression of CML CFU-GM in two, stimulation in three, and no significant effect in two CML patients' samples. (utmb.edu)
Bone marrow consists of stem cells, which are large, "primitive," undifferentiated cells supported by fibrous tissue called stroma. (medscape.com)
There are 2 main types of stem cells and, therefore, the bone marrow consists of 2 types of cellular tissue. (medscape.com)
However, the yellow marrow can revert to red if there is increased demand for red blood cells, such as in instances of blood loss. (medscape.com)
Normally, only mature cells are released from the marrow into the bloodstream. (medscape.com)
These stem cells divide to eventually give rise to red blood cells, platelets, and most white blood cells in the red marrow. (medscape.com)
Bone marrow thus contains blood cells at varying stages of development. (medscape.com)
Illustration of the pelvis to show the site of bone marrow and blood cells derived from bone marrow. (medscape.com)
When the oxygen content of body tissues is low, if there is loss of blood or anemia, or if the number of red blood cells decreases, the kidneys produce and release erythropoietin, a hormone that stimulates the bone marrow to produce more red blood cells. (medscape.com)
Similarly, the bone marrow produces and releases more white blood cells in response to infections, and it produces and releases more platelets in response to bleeding. (medscape.com)
As age progresses, more of the red bone marrow turns into yellow bone marrow and the production of new blood cells becomes more difficult. (medscape.com)
The bone marrow stroma contains mesenchymal stem cells. (medscape.com)

Colony forming assays demonstrated that mice on the SBR diet had 1) less reduction in lymphoid CFU-preB progenitor cells, 2) greater recovery of CFU-preB progenitor cells at 168 h, and 3) less reduction of CFU-GM progenitor cells at 6 h. (nih.gov)
Chronic lymphoid leukaemia and hairy cell leukaemia due to chronic exposure to benzene: Report of three cases. (cdc.gov)
The common stem cell produces 2 other stem cells, the myeloid stem cell and the lymphoid stem cell. (medscape.com)
T lymphocytes originate via lymphoid stem cells that migrate to the thymus and differentiate under the influence of the thymic hormones thymopoietin and thymosin. (medscape.com)

Her publications number more than 300, and she is the editor of four textbooks: Asbestos-related Malignancies, Sarcomas of bone and soft tissue, High-dose cancer therapy that includes pharmacology, hematopoietins and stem cells (three editions), Molecular Targeting in Oncology). (wikipedia.org)
The conventional view of how stem cells differentiate assumes that cells are "instructed" to progress along prescribed signaling pathways. (sciencedaily.com)
The work also suggests a much more efficient way of differentiating stem cells for therapeutic purposes. (sciencedaily.com)
Led by Sui Huang, MD, PhD, a Visiting Associate Professor in the Children's Hospital Boston Vascular Biology Program (now also on the faculty of the University of Calgary), and Hannah Chang, an MD/PhD student in Children's Vascular Biology Program, the researchers examined how blood stem cells "decide" to become white blood cell progenitors or red blood cell progenitors. (sciencedaily.com)
Blood stem cells with low levels of Sca-1 differentiated into red blood cell progenitors seven times more often than cells high in Sca-1 when exposed to erythropoietin, a growth factor that promotes red blood cell production. (sciencedaily.com)
Conversely, when stem cells were exposed to granulocyte--macrophage colony-stimulating factor, which stimulates white blood cell formation, those that were highest in Sca-1 were the most likely to become white cells. (sciencedaily.com)
Yet, in both experiments, all three groups of cells retained characteristics of stem cells. (sciencedaily.com)
Blood stem cells that were low in Sca-1 (and most prone to become red blood cells) had much more GATA1 than did the high- and medium-Sca-1 cells. (sciencedaily.com)
Stem cells high in Sca-1 (and least prone to become red blood cells) had the highest levels of PU.1. (sciencedaily.com)
3) We are defining the properties of cancer stem cells that initiate and sustain the unique disease features of acute leukemias through the use of various adoptive animal models. (stanford.edu)
The aging of hematopoietic stem cells (HSCs) is characterized by the impaired ability of these cells to regenerate and produce blood cells. (medicalnewstoday.com)
A recent study conducted in mice shows that blocking the receptor for the proinflammatory protein IL-1 using the FDA-approved rheumatoid arthritis drug Anakinra may attenuate the deficits in the functioning of hematopoietic stem cells due to aging. (medicalnewstoday.com)
Aging is associated with a decline in the function of the hematopoietic stem cells. (medicalnewstoday.com)
At various times thereafter the hematopoietic stem cells were flushed from female C3Hf/Sed mouse preheated tibia. (elsevierpure.com)
Recent research demonstrates that tumors originate from the Cancer S tem Cells (CSCs) or Tumor Initiated Cells (TIC) [ 1 ], which are called breast cancer stem cells in breast cancer. (omicsonline.org)
BCR-ABL-transformed GMP as myeloid leukemic stem cells. (ca.gov)
During blast crisis of chronic myelogenous leukemia (CML), abnormal granulocyte macrophage progenitors (GMP) with nuclear beta-catenin acquire self-renewal potential and may function as leukemic stem cells (Jamieson et al. (ca.gov)
These results provide further evidence that BCR-ABL-transformed GMP with abnormal beta-catenin activity can function as leukemic stem cells. (ca.gov)
Compared to radiation controls, glucan-treated mice consistently exhibited a more rapid recovery of pluripotent stem cells and committed granulocyte, macrophage, and erythroid progenitor cells. (betaglucan.com)
As needed, the stem cells differentiate to become a particular kind of cell-a white blood cell, red blood cell, or platelet. (medscape.com)
Stem cells exist throughout the life of an individual. (medscape.com)

White blood cells last anywhere from a few hours to a few days, platelets for about 10 days, and red blood cells for about 120 days. (medscape.com)

We also examined the numbers of colony-forming progenitor cells (CFUs) before and after therapy. (springermedizin.at)

Mononuclear cell progeny released from these clusters at and beyond 4 d exhibited distinct dendritic morphology and were actively phagocytic. (silverchair.com)
Macrophages form the largest component of the intestinal mononuclear phagocyte system and play a pivotal role in mediating immune homeostasis ( 2 ). (frontiersin.org)
Results from murine studies ( 2 ) combined with the observation of radiolabelled blood monocytes in the inflamed intestinal mucosa of IBD patients ( 15 ), indicate that increased LP inflammatory mononuclear cells are a result of monocyte recruitment, rather than expansion of a tissue resident macrophage population. (frontiersin.org)
and high glucose in mononuclear cells? (musc.edu)
Toll-like receptor 4 activation in microvascular endothelial cells triggers a robust inflammatory response and cross talk with mononuclear cells via interleukin-6. (musc.edu)

This suggested that EPCs are able to differentiate into endothelial cells and that such cells are incorporated into sites of active angiogenesis including ischemia, tumor angiogenesis, and metastasis in adult organisms [ 16 , 17 ]. (hindawi.com)
It reveals an elegant yet simple system that maintains cells in a stable state, yet enables them to differentiate under the right conditions. (sciencedaily.com)
Instead, it supports the idea that cells differentiate through the collective behavior of multiple genes in a network that ultimately leads to just a few endpoints -- just as a marble on a hilltop can travel a nearly infinite number of downward paths, only to arrive in the same valley. (sciencedaily.com)
But most important, the differences in Sca-1, GATA1 and PU.1 levels across the three cell groups became less pronounced over time, as did the variability in the cells' propensity to differentiate, suggesting that the differences are transient. (sciencedaily.com)
Together, the findings make the case that a slow fluctuation or cycling of gene activity tends to maintain cells in a stable state, while also priming them to differentiate when conditions are right. (sciencedaily.com)

Open-Label Phase II Prospective, Randomized, Controlled Study of Romyelocel-L Myeloid Progenitor Cells to Reduce Infection During Induction Chemotherapy for Acute Myeloid Leukemia. (uchicago.edu)
Effect of beta glucan on white blood cell counts and serum levels of IL-4 and IL-12 in women with breast cancer undergoing chemotherapy: a randomized double-blind placebo-controlled clinical trial. (betaglucan.com)

We exposed healthy mouse lung tissue to radiation before the induction of metastasis and observed a strong enhancement of cancer cell growth. (nature.com)
Myeloid cells promote tumor growth by stimulating tumor angiogenesis, suppressing tumor immunity, and promoting metastasis to distinct sites. (hindawi.com)
In 1971, Dr. Judah Folkman observed that neovascularization occurs around tumors and proposed that new blood vessel growth is necessary to supply nutrients and oxygen to tumor cells during exponential tumor growth [ 6 ]. (hindawi.com)
Specific growth factors, such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), stimulate the proliferation and migration of naturally quiescent endothelial cells, resulting in the formation of new vessel structures during embryonic development and tumor growth [ 7 ]. (hindawi.com)
Upon culture in endothelial growth media, these cells expressed endothelial lineage markers, such as CD31, Tie2, and VEGF receptor 2 (VEGFR2), and incorporated into blood vessels in ischemic tissues. (hindawi.com)
Its functions are to stimulate B cell growth and increase immunoglobulin secretion. (dadamo.com)
BACKGROUND: Recent reports of the utilisation of pyrvinium pamoate (PP), an FDA-approved anti-helminth, have shown that it inhibits pancreatic ductal adenocarcinoma (PDAC) cell growth and proliferation in-vitro and in-vivo in preclinical models. (bvsalud.org)
Clinical manifestations of PNH occur when a HSC clone carrying somatic PIGA mutations acquires a growth advantage and differentiates, generating mature blood cells that are deficient of GPI-anchored proteins. (medscape.com)

arise from a single type of cell called a hematopoietic stem cell - an "adult" multipotent stem cell. (tdmuv.com)

Lenzilumab, a granulocyte-macrophage colony-stimulating factor (GM-CSF) neutralizing antibody, prevents GM CSF from binding to receptors on hematologic progenitor cells. (biospace.com)
Siglecs (sialic acid-binding immunoglobulin-like lectins) are single-pass cell surface receptors that have inhibitory activities on immune cells. (mdpi.com)

Subsequent studies described a VEGFR2 and AC133 expressing subpopulation of these CD34 positive circulating cells that could form endothelial colonies in vitro [ 14 , 15 ]. (hindawi.com)
Progenitor cell function may be measured post-modification by in vitro CFU assays. (stemcell.com)
In spite of the advantages of the in vitro tests, they are not able to mimic the orchestrated role of cells present inperiradicular region and the long-term cytotoxicity presented by the sealers. (bvsalud.org)

However, it is not clear how gut macrophages, relative to recruited blood monocytes and other myeloid cells, contribute to the intestinal inflammatory milieu, nor how macrophages and their monocyte precursors mediate recruitment of other immune cells to the inflamed intestine. (frontiersin.org)
Finally, our data reveal that, independent of inflammation, murine colon macrophages act as a major source of Ccl7 and Ccl8 chemokines that trigger further recruitment of their pro-inflammatory monocyte precursors. (frontiersin.org)
The four stem cell precursors differed markedly in their abilities to develop thermotolerance. (elsevierpure.com)

These cells can participate in inflammatory responses by releasing mediators that attract or activate other cells, contributing to the pathogenesis of allergic and non-allergic diseases. (mdpi.com)
Intestinal macrophages display a "tolerant" phenotype, poorly responsive to toll like receptor (TLR) ligands (vital to avoid triggering inflammatory responses while scavenging commensal bacteria) whilst maintaining a homeostatic environment via IL-10 and PGE 2 production ( 7 ). (frontiersin.org)
Furthermore, the induction of cell death caused by these materials, which is associated with the release of proinflammatory mediators, leads to a persistence of periapical inflammatory reaction and increases the time required for wound healing 5 . (bvsalud.org)
4,6,8,10-11 The behavior of other inflammatory cells present in the periapical region has not been widely assessed, although they also contribute to the intensity of the biological response to these materials. (bvsalud.org)
Macrophages are the prevalent cells in inflammatory infiltrates that respond to sealers 12-13 . (bvsalud.org)

This Technical Bulletin provides instructions for CRISPR-Cas9 genome editing and maintenance of human CD34 + cells, including optimized pre- and post-editing culture conditions and methods to evaluate genome editing efficiency. (stemcell.com)
STUDY DESIGN AND METHODS: The hematopoietic progenitor cell (HPC) and lymphocyte subset (LS) content of approximately 8000 CB units were characterized, and these results were correlated with donor ethnicity, birth weight, gestational age, sex, and type of delivery. (emmes.com)
But existing methods to identify cell of origin from bulk tumor cell samples have been unsuccessful. (news-medical.net)

hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)

Finally, we will comment on the mechanisms regulating myeloid cell recruitment to the tumor microenvironment and on the potential of myeloid cells as new targets for cancer therapy. (hindawi.com)

Mammalian cells require oxygen and nutrients for their survival and are therefore located within 100 to 200 m of blood vessels, the diffusion limit of oxygen. (hindawi.com)
CRISPR-Cas9, an RNA-guided genome editing technology, is revolutionizing cell biology due to the ease and efficiency by which it enables genetic manipulation of mammalian cells. (stemcell.com)

The AML that developed from these different cells of origin had different penetrance and aggressiveness when engrafted in mice, with the stem cell-derived lines being the most aggressive and the committed progenitor lines the least. (news-medical.net)

The score of ALDH1 in luminal epithelial cytoplasm expression is 3, 2, and 1 while myoepithelial cells and stroma expression are 3, 2, and 2 in ADHÃ¯Â¼ÂŒUDH and normal ducts respectively. (omicsonline.org)

Unlike the splenic DC, however, most of the GM-CSF-propagated putative liver DC harvested at 6-10 d expressed only a low level of major histocompatibility complex (MHC) class II (I-Ek), and they failed to induce primary allogeneic responses in naive T cells, even when propagated additionally in GM-CSF and tumor necrosis alpha and/or interferon gamma-supplemented medium. (silverchair.com)
Tumors exhibit significant myeloid cell infiltrates, which are actively recruited to the tumor microenvironment. (hindawi.com)
In this review, we discuss the role of myeloid cells in promoting tumor angiogenesis. (hindawi.com)
Importantly, during tumor progression, myeloid cells are implicated in promoting tumor angiogenesis, causing resistance against antiangiogenic therapies in cancer, and suppressing the immune response during cancer [ 3 - 5 ]. (hindawi.com)
However, in cancer, myeloid cells are largely converted to serve a new master-tumor cells. (aacrjournals.org)
These cells have emerged as an important contributor to tumor progression. (aacrjournals.org)
Reports on the accumulation of immune-suppressive myeloid cells associated with tumor progression were published sporadically beginning in the early 1970s ( 1 ). (aacrjournals.org)
The observations of the accumulation in spleens and tumors of large numbers of these cells with potent immune-suppressive activity were readily reproducible in most murine tumor models. (aacrjournals.org)
Knowing the cell of origin of cancer cells can provide insight into tumor subtypes and possibly diagnostic and therapeutic benefit,' says JAX Assistant Professor Jennifer Trowbridge, Ph.D., the lead author of the study published on July 11 in Nature Communications . (news-medical.net)
Trowbridge hypothesized that analyzing open chromatin in bulk tumor cells could provide a possible improved method to identify cancer cell of origin because of the cell-type specificity of chromatin structure. (news-medical.net)
The INK4b-ARF-INK4a tumor suppressor locus (Figure 1A ) plays a central role in controlling the equilibrium between progenitor cell renewal and cancer risk [ 1 - 8 ]. (biomedcentral.com)

Leukemia is a malignant condition involving the excess production of immature or abnormal leukocytes, which eventually suppresses the production of normal blood cells and results in symptoms related to cytopenias. (msdmanuals.com)

These mature blood cells include red blood cells (erythrocytes), white blood cells (leukocytes), and cells belonging to the myeloid lineage. (medicalnewstoday.com)

Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)

A human cell line established from a diffuse histiocytic lymphoma (HISTIOCYTIC LYMPHOMA, DIFFUSE) and displaying many monocytic characteristics. (umassmed.edu)
Thioredoxin reductase-1 negatively regulates HIV-1 transactivating protein Tat-dependent transcription in human macrophages. (umassmed.edu)
While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
Since the main problem was the leakage of several tones of polychlorinated biphenyls (PCBs) as well as heavy metals Cr and Ni into the environment and groundwater, we decided to evaluate influence of the environmental contamination on eventual changes of genetic constitution of human body cells. (go.jp)
Studies using human hematopoietic progenitor cells demonstrated that AMT was 5- to 7-fold more toxic to human colony-forming units granulocyte-macrophage and burst-forming units erythroid than was AZT. (aspetjournals.org)
These results bear out indications in human data that the stage of a progenitor cell when it becomes transformed to leukemia has an impact on its clinical progression, with earlier-stage cell of origin cancers being more aggressive. (news-medical.net)
In addition, mRNA expression profiles in human and murine colon samples, and in macrophages and monocytes from healthy and inflamed murine colons, were analysed by quantitative PCR (qPCR) and mRNA microarray. (frontiersin.org)
Previous studies have shown the accumulation of human CD14 Hi or CD14 + HLA-DR Int cells in the GI tract lamina propria (LP) during inflammation ( 2 , 6 , 11 - 14 ). (frontiersin.org)

Macrophages are pivotal in coordinating a range of important processes in the intestines, including controlling intracellular infections and limiting damaging inflammation against the microbiota. (frontiersin.org)
Our work suggests that strategies targeting macrophage-mediated monocyte recruitment may represent a promising approach for limiting the chronic inflammation that characterises IBD. (frontiersin.org)

abstract = "We have studied the sensitivities of four hematopoietic stem cell types to heat stress as well as their abilities to develop thermotolerance. (elsevierpure.com)

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). (thermofisher.com)
Applications Tested: The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. (thermofisher.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)

Anatomy17

Organisms4

Chemicals and Drugs14

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes9

Neutrophils10

Differentiation19

Eosinophils4

Fibroblasts5

Common myeloid progenitors1

Dendritic cells8

Bone marrow progenitor cells1

Recombinant1

Populations8

Endothelial progenitor cells3

Transplantation6

Monocyte:macrophage1

Stromal cells7

Lineages3

Colony-form1

Peripheral9

Eosinophil2

Marrow39

Lymphoid4

Stem Cells21

Platelets1

CFUs1

Mononuclear5

Differentiate5

Chemotherapy2

Growth8

Multipotent1

Receptors2

Vitro3

Precursors3

Inflammatory5

METHODS3

Tissues1

Targets1

Mammalian2

Mice1

Stroma1

Tumor11

Suppresses1

Myeloid lineage1

Colonies1

Human8

Inflammation2

Abstract1

Antibody3