Granulocyte hematopoietic progenitor cells hematopoietic myeloid cells. Medical search. Frequent questions

The first stage of the myeloid lineage is a granulocyte - monocyte progenitor (GMP), still an oligopotent progenitor, which then develops into unipotent cells that will later on form a population of granulocytes, as well as a population of monocytes. (wikipedia.org)
Granulocyte lineage determination is regulated by expression of C/EBPα, which is necessary for the transition from CMPs to GMPs and levels of PU.1, that drive the differentation from GMPs to monocytes (high PU.1 levels) or to granulocytes (low PU.1 levels). (wikipedia.org)
These cells are closely related to neutrophils and monocytes. (aacrjournals.org)
Mononuclear myeloid cells include terminally differentiated macrophages and dendritic cells (DC), as well as monocytes, which under inflammatory conditions differentiate in tissues to macrophages and DCs. (aacrjournals.org)
Myeloid cells generated under these conditions, although similar to neutrophils and monocytes in morphology and phenotype, have different genomic and biochemical profiles and functional activity. (aacrjournals.org)
The field started changing in the late 1990s when the Gr1 + CD11b + cellular phenotype was suggested as defining the immune-suppressive myeloid cells in spleens of mice and when these cells were shown to be phenotypically similar but functionally distinct from monocytes and neutrophils ( 2, 3 ). (aacrjournals.org)
CLP granulocytes alsocietypublishing.org monocytes visited the higher the entropy. (lu.se)
It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. (angioproteomie.com)
Although granulocyte production predominates, the neoplastic clone includes red blood cells, megakaryocytes, monocytes, and even some T cells and B cells. (msdmanuals.com)
CD68 is predominately expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, and granulocytes. (biolegend.com)
The non-lethal c-Myb mutation in booreana mice was associated with significantly lower representation of splenic CD8- conventional dendritic cells (cDCs), inflammatory monocytes, and neutrophils compared to wild-type mice. (edu.au)
CFU-E for red blood cells and CFU-GM for cells that form granulocytes and monocytes). (wikilectures.eu)
Parent cells in the lineage that gives rise to MONOCYTES and MACROPHAGES. (ouhsc.edu)
The white blood cells (leukocytes) are further divided into phagocytes or myeloid (neutrophils, eosinophils, basophils, monocytes) and immunocytes or lymphoid (lymphocytes). (medscape.com)

MYB is an essential regulator of primitive human hematopoiesis in human pluripotent stem cell differentiation cultures. (nih.gov)
MYB is well recognized to be a key regulator of definitive hematopoiesis that plays an important role in the maintenance and multilineage differentiation of hematopoietic stem cells (HSCs). (nih.gov)
To explore the role of MYB in human hematopoietic development we have inactivated the gene by bi-allelic TALEN-supported gene targeting in several lines of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), and subjected these cells to hematopoietic differentiation in well-defined cell culture conditions. (nih.gov)
Committed granulopoiesis is regulated by C/EBPε and GFI-1, these two transcriptional factors are important for terminal granulocyte differentiation. (wikipedia.org)
It restricts the cell cycle of immature cells by inhibition of CDK2 and CDK4 and promotes granulocytic differentiation. (wikipedia.org)
DNMT3A is a critical regulator of hematopoietic stem cell (HSC) differentiation and somatic DNMT3A mutations are frequent in hematologic malignancies and clonal hematopoiesis. (haematologica.org)
M, Peterson C. 2018 Haematopoietic stem suggests that during differentiation a stem cell approaches a stable equili- cells: entropic landscapes of differentiation. (lu.se)
set-ups of haematopoietic differentiation. (lu.se)
Single cells in these populations exhibit different com- stem cell differentiation, Shannon information binations of regulator activity that suggest the presence of multiple theory, entropy configurations of a potential differentiation network as a result of multiple entry points into the committed state. (lu.se)
Additionally, edited CD34 + cells can be further cultured in conditions stimulating lineage-specific differentiation by combining StemSpan™ media with expansion supplements designed for the expansion and differentiation of progenitors to erythroid, myeloid, megakaryocyte, natural killer (NK), or T cell lineages. (stemcell.com)
The proliferation and differentiation of pluripotent stem cells give rise to progeny that can populate the entire immunologic and hematopoietic systems through committed progenitors of both the lymphoid and myeloid lineages. (medscape.com)
Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. (cusabio.com)
Sequential rounds of division and differentiation give rise to an enormous amplification of cell numbers, necessary for the production of mature blood cells. (justia.com)
This process of division and differentiation is subject to regulation at many levels to control cell production. (justia.com)
A specific question addressed whether booreana mice contained increased numbers of dendritic-like cells (L-DC subset) recently identified in the spleen, since L-DCs arise in vitro by direct differentiation from HSPCs co-cultured over splenic stroma. (edu.au)
The molecular pathways of normal myeloid differentiation, as well as the mechanisms by which oncogenes disrupt this process, remain poorly understood. (nih.gov)
A major limitation in approaching this problem has been the lack of suitable cell lines that exhibit normal, terminal, and synchronous differentiation in the absence of endogenous oncoproteins and in response to physiologic cytokines, and whose differentiation can be arrested by ectopically expressed human oncoproteins. (nih.gov)
Differentiation was normal and terminal as evidenced by morphology, cell surface markers, gene expression, and functional assays. (nih.gov)
The differentiation of the cells could be arrested by heterologous oncoproteins including AML1/ETO, PML/RARalpha, PLZF/RARalpha, Nup98/HoxA9, and other Hox proteins. (nih.gov)
These myeloid cell lines provide tools for examining the biochemical and genetic pathways that accompany normal differentiation as well as a system in which to dissect how other leukemic oncoproteins interfere with these pathways. (nih.gov)
The differentiation of Socs3-deficient progenitor cells is skewed toward macrophage production in response to G-CSF or IL-6, whereas wild-type progenitor cells produce mainly neutrophils. (nih.gov)
Activation of the canonical Wnt pathway leads to loss of hematopoietic stem cell repopulation and multilineage differentiation block. (ox.ac.uk)
Constitutive activation of canonical Wnt signaling therefore causes multilineage differentiation block and compromised hematopoietic stem cell maintenance. (ox.ac.uk)
We show here that these novel intrathymic DCs are truly myeloid, as they arise from CD34 + early thymic progenitors through CD34 lo intermediates which have lost the capacity to generate T cells, but display myelomonocytic differentiation potential. (ashpublications.org)
These malignancies are further characterized by the maturity and differentiation of the individual cell types and are divided into acute leukemias such as acute myeloid leukemia and acute lymphoblastic leukemia and chronic leukemias such as chronic myeloid leukemia and chronic lymphocytic leukemia. (medscape.com)
Different characteristics of the nuclei and cytoplasm of the cell allow differentiation by instrumentation and microscopy. (medscape.com)

Steady state production of granulocytes is activated after the engulfment of apoptotic granulocytes by tissue macrophages. (wikipedia.org)
The microenvironment, consisting of lymphocytes, macrophages, fibroblasts, endothelial elements, and stroma in which hematopoietic cells reside, creates a regulatory niche that determines the local area network. (medscape.com)
The innate immune system mainly comprises innate immune cells (macrophages: neutrophils, dendritic cells) and complement factors. (biomedcentral.com)
When dendritic cells are activated by inflammatory cytokines such as LPSs and inflammatory cytokines produced by macrophages, they present fragments of pathogens digested intracellularly to T cells and induce the activation of antigen-specific T cells. (biomedcentral.com)
LYMPHOCYTES (B and T cells) derive continuously from the same multipotential stem cells that produce myeloid cells, including erythrocytes, granulocytes and macrophages. (pasteur.fr)
We report the characterization and purification of fetal liver cells that generate clones containing both macrophages and B cells, identified biochemically and morphologically. (pasteur.fr)

This role is fulfilled by specialized cells called dendritic cells. (biomedcentral.com)
In this study, the finding that a significant proportion of all dendritic cells (DCs) resident in vivo in the human postnatal thymus displayed a myeloid-related phenotype prompted us to re-examine the developmental origin of thymic DCs, a cell type hitherto considered to represent a homogeneous lymphoid-derived population. (ashpublications.org)
Dendritic cells (DCs) are hematopoietic-derived highly specialized antigen-presenting cells (APCs) that display potent ability to induce both specific immune responses and deletion of potentially autoreactive T cells. (ashpublications.org)

However, inactivation of MYB severely abrogated proliferation of the primitive erythroid and mixed erythroid-macrophage-megakaryocyte progenitors. (nih.gov)
Bone marrow failure syndrome (BMFS) is a group of disorders that may manifest as single cytopenia (eg, erythroid, myeloid, or megakaryocytic) or as pancytopenia. (medscape.com)
This point mutation led to increased numbers of early hematopoietic stem and progenitor cells (HSPCs), with a subsequent reduction in the development of B cells, erythroid cells, and neutrophils, and increased numbers of myeloid cells and granulocytes. (edu.au)
CRISPR/Cas9-mediated TPM1 knockout in human induced pluripotent stem cells (iPSCs) enhanced hematopoietic progenitor development, increasing total megakaryocyte and erythroid cell yields. (biomedcentral.com)
Loss of hematopoietic stem cell function was associated with decreased expression of Cdkn1a (encoding the cell cycle inhibitor p21(cdk)), Sfpi1, Hoxb4 and Bmi1 (encoding the transcription factors PU.1, HoxB4 and Bmi-1, respectively) and altered integrin expression in Lin(-)Sca-1(+)c-Kit(+) cells, whereas PU.1 was upregulated in erythroid progenitors. (ox.ac.uk)
Production of G/M or Meg increases while lymphoid and erythroid (E) production decreases, resulting in higher risk of infection, stroke, heart attack, anemia, etc. in the elderly and various complications in myeloid leukemia patients. (thekanglab.org)
The presence of Meg-biased HSCs and Meg-biased MPP2 generated from HSCs suggests that MPP2 would need to shut down the Meg program to be able to produce erythroid cells. (thekanglab.org)
Moreover, in human studies, JAK2V617F has been identified in the stem cell-enriched CD34 + CD38 − CD90 + Lin − compartment, common myeloid progenitors, granulocyte-monocyte precursors, and megakaryocyte-erythroid precursors as well as natural killer cells, B cells, and T cells in some patients, confirming its origin in an early hematopoietic progenitor. (oncohemakey.com)

Granulocytic myeloid cells include populations of terminally differentiated polymorphonuclear neutrophils, eosinophils, basophils, and mast cells. (aacrjournals.org)
LA JOLLA, CA-Neutrophils-short-lived, highly mobile and versatile-outnumber all other immune cells circulating through the blood stream. (lji.org)
Yet, despite the cells' abundance, the progenitor cell that only gives rise to neutrophils had eluded all efforts to track it down. (lji.org)
Now, researchers at La Jolla Institute for Immunology identified a progenitor of neutrophils in the bone marrow of mice and humans and tied it to cancer-promoting activities. (lji.org)
Neutrophils (bottom right) are derived from a newly discovered unipotent neutrophil progenitor (top left). (lji.org)
Neutrophils are among the first immune cells to arrive at the scene when pathogens breach the body's physical barriers. (lji.org)
Despite their abundance and importance, researchers had been unable to trace neutrophils' lineage to their origin in the bone marrow, where multipotent hematopoietic stem cells give rise to a series of increasingly specialized progenitor cells that eventually differentiate into their target cell types, including red blood cells, lymphocytes and neutrophils. (lji.org)
Over the years, people identified different white blood cell progenitors but the one that was missing was the neutrophil progenitor because we didn't have the tools to pull the populations apart," says postdoctoral researcher and the study's first author, Yanfang Peipei Zhu, Ph.D. "Now, we can study disease where neutrophils execute unique and important functions and investigate further how certain subsets of them promote tumor growth. (lji.org)
CyTOF allowed Zhu to simultaneously analyze 39 surface markers known to pinpoint hematopoietic stem and progenitor cells, transient myeloid precursors, and terminally differentiated myeloid cells, especially granulocytes, the subset of immune cells neutrophils belong to. (lji.org)
In vivo adoptive transfer approaches confirmed that this progenitor gives rise only to neutrophils, bringing a decades-long search for a committed neutrophil progenitor to its successful conclusion. (lji.org)
granulocytes (neutrophils, eosinophils and basophils) are involved in overcoming infection, parasites and tumors. (justia.com)
G-CSF also modulates multiple differentiated functions of human neutrophils, including enhanced oxidative metabolism in response to f- Met-Leu-Phe (f-MLP), increased antibody-dependent cell-mediated cytotoxicity (ADCC), and augmented arachidonic acid release in response to ionophore and chemotactic agents. (ashpublications.org)
These effects are all maximal at a concentration of 100 to 500 pmol/L. Using 125I-labeled recombinant human G-CSF, high affinity binding sites were identified on human neutrophils, the myeloid leukemia cell lines KG-1 and HL-60, and the small cell carcinoma cell lines, H128 and H69. (ashpublications.org)
Decreased specific binding of 125l-G-CSF by human neutrophils was consistently observed in the presence of excess unlabeled human granulocyte-macrophage colony-stimulating factor (GM-CSF), suggesting competition or down modulation by GM-CSF of the G- CSF receptor. (ashpublications.org)
FYLNETRA™ is used to treat neutropenia (low neutrophils which are a type of white blood cells that fight infection) which is commonly experienced by patients undergoing chemotherapy. (tarsadia.com)
Expression of high levels of immune cells including neutrophils has been associated with detrimental outcome in several solid tumors and new strategies to decrease their presence and activity are currently under clinical development. (biomedcentral.com)
Examples for such strategies include inhibition of CXCR1 and CXCR2 to decrease migration of neutrophils to tumoral areas or the inhibition of granulocyte colony stimulating factor to decrease the amount of neutrophils which has shown efficacy in preclinical models. (biomedcentral.com)
Neutrophilic granulocytes (neutrophils) account for 50-70% of all leukocytes and depend on a sequential process of maturation in the bone marrow that provokes the conversion of myeloblasts to segmented neutrophils [ 7 ]. (biomedcentral.com)

The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)

1) Granulocyte lineage determination and 2) Committed granulopoiesis. (wikipedia.org)
The main cytokines driving granulopoiesis are: GM-CSF (formation of GMPs from CMPs), G-CSF (commitment to the granulocyte lineage, formation of myeloblasts from GMPs), IL-3 (enhances the production of GM-CSF and G-CSF) and SCF. (wikipedia.org)
A malignancy in the lymphoid lineage that includes white blood cells such as T lymphocytes and B lymphocytes. (medgadget.com)
And, malignancy in the myeloid lineage that includes precursor cells to red blood cells, platelets and white blood cells such as granulocytes. (medgadget.com)
CML ensues when an abnormal pluripotent hematopoietic progenitor cell initiates excessive production of all myeloid lineage cells, primarily in the bone marrow but also in extramedullary sites (eg, spleen, liver). (msdmanuals.com)
These mature blood cells include red blood cells (erythrocytes), white blood cells (leukocytes), and cells belonging to the myeloid lineage. (medicalnewstoday.com)
Although similar methods have detected committed pre-B cells in mouse fetal liver, earlier progenitors with additional non-B lineage options have not been demonstrated in normal tissues. (pasteur.fr)
Their dual potential and unrearranged immunoglobulin loci place the precursors before exclusive B-lineage commitment in the haematopoietic hierarchy. (pasteur.fr)
The availability of such cells in purified form will allow direct study of lineage choice in cells having both lymphoid and non-lymphoid options. (pasteur.fr)
Hematopoietic stem cells (HSCs) and downstream lineage-biased multipotent progenitors (MPP) tailor blood production. (thekanglab.org)
We study cell fate decision and lineage specification mechanisms in HSCs and MPPs to modulate lineage output in disease and aging context for therapeutic purposes. (thekanglab.org)
While being much smaller compartments than MPP4 at steady-state, MPP2 and MPP3 are transiently expanded in regenerative conditions and are essential for rebuilding the myeloid lineage. (thekanglab.org)
Blood production skews towards myeloid lineage upon aging and myeloid malignancies with expanded MPP2 and MPP3. (thekanglab.org)
Moreover, morphologic and flow cytometric analyses of the transgenic colonies, but not those derived from normal BM, revealed the presence of granulocyte/macrophage colony forming units expressing the HDAC9 transgene, suggesting a lympho-myeloid lineage switch. (sunderland.ac.uk)

We also demonstrate that phenotypically and functionally equivalent myeloid precursors devoid of T-cell potential do exist in vivo in the postnatal thymus. (ashpublications.org)
1-5 In mice, DCs bearing the myeloid marker CD11b (Mac-1), but lacking CD8α, derive efficiently from myeloid precursors and represent the major DC subset in the periphery. (ashpublications.org)

Human granulocyte colony-stimulating factor (G-CSF) is a regulatory glycoprotein that stimulates the production of neutrophilic granulocytes from committed hematopoietic progenitor cells both in vitro and in vivo. (ashpublications.org)
and that (b) granulocyte- colony-stimulating factor (G-CSF) significantly increases its expression at the gene and protein levels in MNC and PMN. (razikurd.ir)
This report describes clonal, granulocyte-macrophage colony-stimulating factor-dependent myeloid cell lines that exhibit these properties. (nih.gov)
3 Following exogenous stimulation, such as chemotherapy or using growth factors such as granulocyte colony stimulating factor (G-CSF) and filgrastim, the number of HSCs in the peripheral blood increases, either becoming on par or even exceeding the number in the bone marrow. (bdbiosciences.com)
Granulocyte colony-stimulating factor (G-CSF) and interleukin-6 (IL-6) play key roles in regulating emergency granulopoiesis and inflammation, and are both negatively regulated by the inducible intracellular protein suppressor of cytokine signaling-3 (Socs3). (nih.gov)
Maturation depends on different stimulating factors including the granulocyte-macrophage-colony stimulating factor (GM-CSF) and the granulocyte-colony stimulating factor (G-CSF), two of the most relevant growth factors that control such maturation process. (biomedcentral.com)
Moreover, although interleukin 7 (IL-7) supports the generation of such myeloid intermediates, we show that their developmental branching from the main intrathymic T-cell pathway is linked to the up-regulation of the myelomonocytic granulocyte macrophage-colony-stimulating factor (GM-CSF) receptor, to the down-regulation of the IL-7 receptor and to the lack of pre-T-cell receptor α (pTα) gene transcriptional activation. (ashpublications.org)
Sargramostim is a colony stimulating factor that is FDA approved for the treatment of granulocytopenia following induction chemotherapy in acute myelogenous leukemia , mobilization and following transplantation of autologous peripheral blood progentior cells, myeloid reconstitution after autologous bone marrow transplantation, myeloid reconstitution after allogeneic bone marrow transplantation and bone marrow transplantation failure or engraftment delay. (wikidoc.org)

They began with five distinct, normal cell types found in the bone marrow in both mice and humans: long-term hematopoietic stem cells (HSCs), short-term HSCs, multipotent progenitors, common myeloid progenitors and granulocyte macrophage progenitors. (news-medical.net)
The more mature LSC population most closely mirrors normal granulocyte-macrophage progenitors (GMP) and the immature LSC population a previously uncharacterized progenitor functionally similar to lymphoid-primed multipotential progenitors (LMPPs). (ox.ac.uk)
Mice with Socs3 deleted specifically in hematopoietic cells succumb to severe neutrophil and macrophage-driven inflammation by 1 year of age, and responses to G-CSF are grossly exacerbated. (nih.gov)
Previously, megakaryocyte (Meg)-biased MPP2 and granulocyte/macrophage (G/M)-biased MPP3 were identified and characterized as novel myeloid-biased MPP subsets. (thekanglab.org)
ER high /FcgR + MPP3 is a G/M primed MPP3 subset with higher cytokine secretory activity serving as a reservoir for rapid granulocyte macrophage progenitor (GMP) production. (thekanglab.org)
Monocyte-Macrophage Precursor Cells" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ouhsc.edu)
This graph shows the total number of publications written about "Monocyte-Macrophage Precursor Cells" by people in this website by year, and whether "Monocyte-Macrophage Precursor Cells" was a major or minor topic of these publications. (ouhsc.edu)
Below are the most recent publications written about "Monocyte-Macrophage Precursor Cells" by people in Profiles. (ouhsc.edu)

It has also been attributed to an inhibitory activity on hematopoietic progenitor cells that give rise to granulocyte and monocyte lineages. (rockland.com)

The many distinct sorts of mature blood cells, like red blood cells for carrying oxygen, white blood cells for immune protection and platelets for wound clotting. (medgadget.com)
CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. (angioproteomie.com)
Other cell types derived from hematopoietic stem cells include platelets and erythrocytes. (justia.com)
Many of the clinical complications of PV relate directly to the increase in blood viscosity associated with red cell mass elevation and indirectly to the increased turnover of red cells, leukocytes, and platelets with the attendant increase in uric acid and cytokine production. (basicmedicalkey.com)
Identifying causal variants and genes from human genetic studies of hematopoietic traits is important to enumerate basic regulatory mechanisms underlying these traits, and could ultimately augment translational efforts to generate platelets and/or red blood cells in vitro. (biomedcentral.com)

Transposon-mediated rescue of MYB expression in MYB-null cells efficiently restored both the primitive hematopoietic progenitors and immature myeloid cells. (nih.gov)
Chronic myeloid leukemia (CML) occurs when a pluripotent stem cell undergoes malignant transformation and clonal myeloproliferation, leading to a striking overproduction of mature and immature granulocytes. (msdmanuals.com)
Загальні відомості про лейкоз Leukemia is a malignant condition involving the excess production of immature or abnormal leukocytes, which eventually suppresses the production of normal blood cells and results in symptoms. (msdmanuals.com)
CD34, a transmembrane phosphoglycoprotein, is present on immature hematopoietic precursor cells and all hematopoietic colony-forming cells in bone marrow and blood, including unipotent and pluripotent progenitor cells. (bdbiosciences.com)
Immunophenotypic analysis of B-cells isolated from bone marrow (BM) revealed an absence of cells expressing the pre-B/immature-B cell markers normally associated with C-E Hardy's fractions. (sunderland.ac.uk)
Taken together, these data challenge the current view that the thymus is colonized by a lymphoid-restricted progenitor and provide evidence that a more immature precursor population with lymphoid and myelomonocytic potential is actually seeding the human postnatal thymus. (ashpublications.org)

03:06 cell functionally in FINA? (yale.edu)
They are functionally distinct from the lymphoid-primed MPP4 and are geared towards the preferential production of myeloid cells with low contribution to the lymphoid lineages. (thekanglab.org)

The AML that developed from these different cells of origin had different penetrance and aggressiveness when engrafted in mice, with the stem cell-derived lines being the most aggressive and the committed progenitor lines the least. (news-medical.net)
Here we show that gasdermin C (GSDMC) sensitized tumor cells to PARPi in vitro and in immunocompetent mice and caused durable tumor regression in an immune-dependent manner. (bvsalud.org)
Finally, the induction of MHC II may potentiate immune memory by impairing leukemic engraftment in mice previously cured with dasatinib, after re-inoculation of leukemia cells. (bvsalud.org)
A recent study in mice shows that aging is linked to the inflammation of cells in the bone marrow. (medicalnewstoday.com)
A recent study conducted in mice shows that blocking the receptor for the proinflammatory protein IL-1 using the FDA-approved rheumatoid arthritis drug Anakinra may attenuate the deficits in the functioning of hematopoietic stem cells due to aging. (medicalnewstoday.com)
In the present study, the researchers performed several analysis in the hindlimb, forelimb, and pelvic bones from young and old mice to examine the impact of aging on the interaction between the bone marrow niche and hematopoietic cells. (medicalnewstoday.com)
The present study found that aged mice showed a decrease in the population of mesenchymal stromal cells and osteoprogenitor cells that are involved in bone formation in the endosteum. (medicalnewstoday.com)
In contrast, there was an increase in the number of mesenchymal stromal cells in the central marrow of aged mice. (medicalnewstoday.com)
Booreana mice carrying the c-Myb308G point mutation were analyzed to determine changes in early hematopoiesis in the bone marrow and among mature cells in the periphery. (edu.au)
Studies of CD43-deficient mice indicate that CD43 participates in the negative regulation of T-cell activation and adhesion. (bdbiosciences.com)
In order to examine the role of HDAC9 in the lymphoid development and pathogenesis of lymphoid malignancies we used Ig heavy chain enhancer (Eμ), which drives gene expression from early stages of B-cell development, to ectopically express HDAC9 in transgenic mice. (sunderland.ac.uk)
Hemizygous and homozygous mice expressing Flag epitope tagged human HDAC9 (fHDAC9) transgene display throughout their lifespan altered B-cell development. (sunderland.ac.uk)
Furthermore, a subgroup of homozygous Eμ-fHDAC9 mice (n=16) developed tumours (81%) at middle age, and present with enlarged lymph nodes (6%) and abnormal hematopoietic elements in peripheral blood and BM. (sunderland.ac.uk)
Taken together these data suggest that HDAC9 plays a role in B-cell maturation and its ectopic expression in early B-cells leads to perturbation of normal B-cell development, possibly predisposing transgenic mice to tumorigenesis. (sunderland.ac.uk)

Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways. (cusabio.com)
The bcr-abl oncoprotein has uncontrolled tyrosine kinase activity, which deregulates cellular proliferation, decreases adherence of leukemia cells to the bone marrow stroma, and protects leukemic cells from normal programmed cell death (apoptosis). (msdmanuals.com)
PMF is characterized by proliferation predominantly of bone marrow megakaryocytes and granulocytes, resulting in deposition of fibrous connective tissue, and often associated with peripheral blood leukoerythroblastosis, cytopenias, and constitutional symptoms. (oncohemakey.com)
Interleukin-3, which promotes the proliferation of both pluripotent stem cells and unipotent progenitor cells. (wikilectures.eu)
These are the malignant proliferations of abnormal clones of white blood cells within the bone marrow that are broadly categorized into lymphoid and myeloid neoplasms depending on the type of white cell proliferation. (medscape.com)
ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)

FYLNETRA is a leukocyte growth factor indicated to decrease the incidence of infection, as manifested by febrile neutropenia, in patients with non-myeloid malignancies receiving myelosuppressive anti-cancer drugs associated with a clinically significant incidence of febrile neutropenia. (tarsadia.com)
The possibility that pegfilgrastim products act as a growth factor for any tumor type, including myeloid malignancies and myelodysplasia, diseases for which pegfilgrastim products are not approved, cannot be excluded. (tarsadia.com)
30,000 patients with blood-related malignancies receive HDC, which, if the response is satisfactory, could subsequently be followed by hematopoietic stem cell transplantation (HSCT). (bdbiosciences.com)
For many hematopoietic malignancies, collection and infusion of CD34+ hematopoietic stem/progenitor cells following chemotherapy is critical. (bdbiosciences.com)
Wnt signaling increases hematopoietic stem cell self-renewal and is activated in both myeloid and lymphoid malignancies, indicating involvement in both normal and malignant hematopoiesis. (ox.ac.uk)

The cells were harvested at different time points for cell-sorting based on the expression of MYB-YFP and CD34 cell surface marker on day 6 and day 12. (nih.gov)
This Technical Bulletin provides instructions for CRISPR-Cas9 genome editing and maintenance of human CD34 + cells, including optimized pre- and post-editing culture conditions and methods to evaluate genome editing efficiency. (stemcell.com)
While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
The ArciTect™ CRISPR-Cas9 RNP is then prepared and delivered into CD34 + cells using electroporation, and cells are plated in StemSpan™ SFEM II supplemented with StemSpan™ CD34 + Expansion Supplement post-electroporation. (stemcell.com)
Interferon-gamma-mediated pathways are induced in human CD34(+) haematopoietic stem cells. (bsb-bg.eu)
2 Evaluation of harvest adequacy requires the use of reliable progenitor cell assays and this is usually achieved by CD34 cell counting using flow cytometry. (bdbiosciences.com)
4 CD34 expression is historically related to hematopoietic cells and it is considered as the marker of HSCs. (bdbiosciences.com)
An accurate measurement of CD34 is critical for dose requirement protocols in stem cell transplantation. (bdbiosciences.com)
6 Fluorochrome-conjugated monoclonal antibodies directed against CD34 molecule can be used to identify CD34+ cells by flow cytometry. (bdbiosciences.com)
The BD ® Stem Cell Enumeration (SCE) Kit provides simultaneous enumeration of viable dual-positive CD45+/CD34+ hematopoietic stem cell populations in CD34+ absolute counts (cells/µL) as well as the percentage of the total viable leucocyte count that is CD34+ (%CD34). (bdbiosciences.com)
Single-platform flow cytometric absolute cell counting protocols have been shown to provide increased robustness of CD34 enumeration by limiting potential sources of imprecision. (bdbiosciences.com)

However, in cancer, myeloid cells are largely converted to serve a new master-tumor cells. (aacrjournals.org)
These cells have emerged as an important contributor to tumor progression. (aacrjournals.org)
Reports on the accumulation of immune-suppressive myeloid cells associated with tumor progression were published sporadically beginning in the early 1970s ( 1 ). (aacrjournals.org)
The observations of the accumulation in spleens and tumors of large numbers of these cells with potent immune-suppressive activity were readily reproducible in most murine tumor models. (aacrjournals.org)
Knowing the cell of origin of cancer cells can provide insight into tumor subtypes and possibly diagnostic and therapeutic benefit,' says JAX Assistant Professor Jennifer Trowbridge, Ph.D., the lead author of the study published on July 11 in Nature Communications . (news-medical.net)
But existing methods to identify cell of origin from bulk tumor cell samples have been unsuccessful. (news-medical.net)
Trowbridge hypothesized that analyzing open chromatin in bulk tumor cells could provide a possible improved method to identify cancer cell of origin because of the cell-type specificity of chromatin structure. (news-medical.net)
PARPi treatment triggered GSDMC/caspase-8-mediated cancer cell pyroptosis (CCP) that enhanced PARPi killing of tumor cells. (bvsalud.org)
GSDMC-mediated CCP increased memory CD8+ T cell population in lymph node (LN), spleen, tumor, and thus promoted cytotoxic CD8+ T cell infiltration in tumor microenvironment. (bvsalud.org)
Mechanistically, we found that treatment with dasatinib significantly increased MHC II expression on the surface of antigen-presenting cells (APC) in a tumor microenvironment-independent fashion and caused influx of APC cells into the leukemic bone marrow. (bvsalud.org)
Galectin-9 (Gal-9), a member of the ß-galactoside-binding protein family, has been demonstrated to induce T-cell death and promote immunosuppression in the tumor microenvironment. (bvsalud.org)
Their study , published in the August 28, 2018, issue of Cell Reports, found that neutrophil progenitors promote tumor growth and that the frequency of the usually rare cell increases dramatically in the blood of human melanoma patients. (lji.org)
We found that this particular subset of progenitor cells that promotes tumor growth and shows up in high numbers in melanoma patients. (lji.org)
When transferred in mouse cancer models, both the murine and human neutrophil progenitors promoted tumor growth. (lji.org)
Classically, therapies have been designed against molecular alterations that drive the transformation of normal cells into tumor ones [ 1 ]. (biomedcentral.com)

3 Autologous stem cell transplantation almost exclusively uses peripheral blood, while for allogeneic SCT, T-lymphocytes in the peripheral blood could pose some danger. (bdbiosciences.com)

Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
Specifically, methods are disclosed for mobilizing hematopoietic stem cells into a subject's peripheral blood. (justia.com)
In particular, embodiments of the method involve inhibition of both Rac1 and Rac2 GTPases to increase the numbers of hematopoietic stem cells into a subject's peripheral blood of a subject. (justia.com)
Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
Human peripheral blood mononuclear cells were fixed, permeabilized and intracellularly stained with anti-human CD68 (clone Y1/82A) PerCP/Cyanine5.5 (filled histogram) or mouse IgG2b, κ PerCP/Cyanine5.5 isotype control (open histogram). (biolegend.com)
Influence of cobalt chloride on mouse peripheral blood cells. (bsb-bg.eu)
FYLNETRA is not indicated for the mobilization of peripheral blood progenitor cells for hematopoietic stem cell transplantation. (tarsadia.com)

Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. (nkmaxbio.com)
Mesenchymal stromal cells can divide and differentiate into a number of cell types, including bone, cartilage, and adipocytes. (medicalnewstoday.com)
Blood cell formation is the process in which stem cells differentiate into the red or white blood cells. (wikilectures.eu)
A pluripotent stem cell can generate more stem cells (self-renewal) or differentiate into lymphoid or myeloid stem cells, becoming committed progenitor cells/unipotent stem cells. (wikilectures.eu)

These patterns were also reflected in the frequency of leukemia-initiating cells in each cell line, with HSCs having the highest frequency and committed progenitors having the lowest. (news-medical.net)
The aging of hematopoietic stem cells (HSCs) is characterized by the impaired ability of these cells to regenerate and produce blood cells. (medicalnewstoday.com)
Besides producing daughter HSCs after cell division, HSCs also produce different progenitor cell types that, in turn, divide to produce specific types of mature blood cells. (medicalnewstoday.com)
Besides housing hematopoietic stem cells and their progeny, the endosteum and the central marrow also contain distinct cell populations that express molecules or secrete proteins, such as cytokines that regulate the formation, development, and function of HSCs and progenitor cells. (medicalnewstoday.com)
The study found that bone marrow niche cells that support the functioning of HSCs played a key role in blood aging. (medicalnewstoday.com)
Flow cytometric enumeration of CD34+ HSCs and progenitor cells is an established method for the evaluation of bone marrow and stem cell grafts. (bdbiosciences.com)
Previously, I showed that high Wnt and low Notch signaling activities make HSCs produce myeloid-biased MPP2 and MPP3, especially G/M-primed MPP3 subset. (thekanglab.org)

The progenitor cells progressively lose their ability to divide and finally become terminally-differentiated blood cells. (wikilectures.eu)

In particular, we focus on the therapeutic potential of endothelial progenitor cells as an exciting new option for the treatment of ischaemic diseases. (biomedcentral.com)

Every cancer starts with a single cell, and Jackson Laboratory (JAX) researchers have found a precise and reliable way -- whole-genome profiling of open chromatin -- to identify the kind of cell that leads to a given case of leukemia, a valuable key to cancer prognosis and outcome. (news-medical.net)
Her lab worked with a mouse model of acute myeloid leukemia (AML) driven by expression of MLL-AF9, a fusion oncogene formed by a chromosome translocation between human chromosomes 9 and 11. (news-medical.net)
These results bear out indications in human data that the stage of a progenitor cell when it becomes transformed to leukemia has an impact on its clinical progression, with earlier-stage cell of origin cancers being more aggressive. (news-medical.net)
The most commonly diagnosed blood cancers are non-Hodgkin lymphoma, chronic lymphocytic leukemia, acute myeloid leukemia, acute lymphoblastic leukemia and multiple myeloma. (medgadget.com)
Hematologic Malignancie market report is segmented on the basis of type, therapy and by regional & country level.Based upontype, Hematologic Malignancie market is classified intoLeukemia, Acute Lymphocytic Leukemia, Chronic Lymphocytic Leukemia, Acute Myeloid Leukemia, Chronic Myeloid Leukemia, Lymphoma,Multiple Myeloma and Others. (medgadget.com)
In addition to these basic issues concerning leukemia pathogenesis, we are devising new diagnostic procedures for detecting and monitoring leukemia patients based on molecular genetic abnormalities in the malignant cells. (stanford.edu)
We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)
Immunotherapy, in the form of hematopoietic stem cell transplantation (HSCT), has been part of the standard of care in the treatment of acute leukemia for over 40 years. (bvsalud.org)
Recently, loss of MHC class II (MHC II) was shown to be a mechanism of immune escape in patients with acute myeloid leukemia after HSCT. (bvsalud.org)
Here we report that treatment with the tyrosine kinase inhibitor, dasatinib, and an anti-PD-1 antibody in preclinical models of Philadelphia chromosome positive B-cell acute lymphoblastic leukemia is highly active. (bvsalud.org)
K-562 is an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis. (nkmaxbio.com)
The Philadelphia (Ph) chromosome is present in 90 to 95% of cases of chronic myeloid leukemia. (msdmanuals.com)
Coexistence of LMPP-like and GMP-like leukemia stem cells in acute myeloid leukemia. (ox.ac.uk)
We show that in ∼80% of primary human CD34+ acute myeloid leukemia (AML), two expanded populations with hemopoietic progenitor immunophenotype coexist in most patients. (ox.ac.uk)
Interestingly, chemotherapy, including hydroxyurea, has been associated with acute leukemia in JAK2 V617F-negative stem cells in some PV patients. (basicmedicalkey.com)
Myelodysplastic Syndrome (MDS) and Acute Myeloid Leukemia (AML): Monitor patients with breast and lung cancer using FYLNETRA in conjunction with chemotherapy and/or radiotherapy for signs and symptoms of MDS/AML. (tarsadia.com)
HDC with transplant is now standard therapy in multiple myeloma, acute myeloid leukemia in first remission, and intermediate-grade non-Hodgkin's lymphoma. (bdbiosciences.com)
Autologous bone marrow transplantation (ABMT), whereby the patient's own marrow is used as the source of stem cell rescue, is potentially curative in patients with acute myeloid leukemia (AML). (arizona.edu)
Although these disorders share certain clinical features-including bone marrow hypercellularity, frequent splenomegaly, and risks of thrombosis, hemorrhage, and transformation to acute myeloid leukemia (AML)-they also show important phenotypic differences. (oncohemakey.com)
Decreased WBC count, leukopenia, is seen when supply is depleted by infection or treatment such as chemotherapy or radiation therapy, or when a hematopoietic stem cell abnormality does not allow normal growth/maturation within the bone marrow, such as myelodysplastic syndrome or leukemia. (medscape.com)

The common origin of the two cell types was shown by culture of single precursor cells. (pasteur.fr)

The first unipotent cell in granulopoiesis is a myeloblast. (wikipedia.org)
Committed granulopoiesis consists of maturation stages of unipotent cells. (wikipedia.org)

The chronic myeloproliferative neoplasms (MPNs) are clonal disorders characterized by overproduction of mature myeloid cells. (oncohemakey.com)

02:47 and red blood cells in vitro. (yale.edu)
Progenitor cell function may be measured post-modification by in vitro CFU assays. (stemcell.com)
G-CSF receptor numbers ranged between 138 and 285 sites per cell with a kd of 77 to 140 pmol/L, consistent with the concentrations of G-CSF that elicit biologic responses in vitro. (ashpublications.org)
Cost-effective blood cell generation will require novel strategies based on better knowledge of underlying mechanisms driving in vitro development. (biomedcentral.com)
In vitro hematopoietic systems might be improved by identifying novel factors from human genetic studies. (biomedcentral.com)
Variables to be examined include agent concentrations, incubation duration and temperature, cell density, sequence of treatment, and number of cycles of in vitro treatment. (arizona.edu)
In vitro functional clonogenic assays for IL-7 responsive BM-derived B-cell progenitors demonstrated an increase (∼50%) in colony numbers in the transgenic BM. (sunderland.ac.uk)

The relationships between normal and leukemic stem/progenitor cells are unclear. (ox.ac.uk)
In this report, we show that biosynthetic (recombinant) human G-CSF enhances colony formation by normal human bone marrow and the human myeloid leukemic cell lines, HL-60 and KG-1, as well as nonhematopoietic small cell lung cancer lines, H128 and H69. (ashpublications.org)
However, the presence of residual occult viable leukemic cells in the marrow autograft limits the therapeutic effectiveness of ABMT in AML. (arizona.edu)
Hopkins Oncology Center (JHOC), Baltimore, MD, will evaluate novel ex vivo marrow purging regimens by comparison of the effects of incubation with graded concentrations of immunologic and/or pharmacologic agents on the growth of normal marrow and leukemic cells, using an authentic animal model of human AML (the LBN hybrid rat). (arizona.edu)

This result confirmed the bone marrow origin of progenitors for these subsets since c-Myb is essential for their development. (edu.au)
These subsets may derive from different progenitors than those in bone marrow, and are potentially established in the spleen during embryogenesis. (edu.au)
While our initial manuscript focused on our unexpected finding that committed progenitor subsets could not be identified in mitotic progenitors, all reviewers requested further analysis of when distinct interneuron subsets first emerge during development, and how heterogeneity in the eminences could be connected to later timepoints. (authorea.com)

Tri- and bipotential myeloid intermediates between the multipotential stem cells and later unipotential cells have been identified using clonal methods in culture. (pasteur.fr)
The MPNs comprise a set of clonal HSC disorders, characterized by the overproduction of 1 or more mature myeloid cell types. (oncohemakey.com)

The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
In many cases, successfully mobilization is not effected in the patient and inadequate numbers of hematopoietic stem cells are harvested from these patients using current methods. (justia.com)
Quantitating the CD34+ cell population can also be useful during mobilization as well as for determining the optimal timing of apheresis sessions to make sure that enough CD34+ cells have been harvested. (bdbiosciences.com)
If adequate numbers of progenitor cells are not collected, other mobilization therapy should be considered. (wikidoc.org)

Granulopoiesis (or granulocytopoiesis) is a part of haematopoiesis, that leads to the production of granulocytes. (wikipedia.org)
Steady state granulopoiesis is a term used to describe the normal daily production of granulocytes. (wikipedia.org)
C/EBPβ enhances the production of granulocytes by promoting progression of the cell cycle of myeloid progenitors at accelerated rate, therefore generating sufficient amount of new granulocyte to fight the insult. (wikipedia.org)

ADAR1 silencing in breast cancer cells leads to increased apoptosis, suggesting an anti-apoptotic function that promotes cancer progression. (cancerindex.org)
Mutations in TET2, other epigenetic regulators, and other regulators of cytokine signaling are not specific to the classic myeloproliferative neoplasms (MPNs) but may influence prognosis and play roles in hematopoietic stem cell (HSC) dysregulation and progression to accelerated or blast-phase disease. (oncohemakey.com)
Sargramostim should be discontinued immediately if blast cells appear or disease progression occurs. (wikidoc.org)

We also observed differential effects of the four mutants on progenitors, myeloid cells and megakaryocytes. (biomedcentral.com)

They can also be used for increasing white blood cell survival and for chemotherapy. (justia.com)
After incubation, cells will be plated for CFU-GM and CFU-Leuk assays to determine survival of committed hematopoietic progenitors and log-kill of AML cells, and optimal conditions for antileukemic effect with stem-cell sparing. (arizona.edu)

In addition, MYB-negative hESC/iPSC lines demonstrated major defects in myeloid cell development and completely failed to generate mature granulocytes. (nih.gov)

These may be classified into those that result in pancytopenia and those limited to failure of 1 or 2 hematopoietic lineages. (medscape.com)

Global gene expression profiling shows the LSC populations are molecularly distinct and resemble normal progenitors but not stem cells. (ox.ac.uk)
1-3 These nonoverlapping functions have been proposed to result from the actions of 2 major DC populations which have been characterized as myeloid and lymphoid DCs, respectively, on the basis of their anatomical localization and cell-surface phenotypes and, ultimately, of their distinct developmental origin. (ashpublications.org)

The ability to genetically manipulate hematopoietic stem and progenitor cells (HSPCs) in particular has significantly advanced our understanding of the mechanisms that regulate hematopoiesis and is contributing to the development of novel cellular therapies. (stemcell.com)
The bone marrow is the main site for hematopoiesis , the process of new blood cells forming via the hematopoietic stem cells (HSC) dividing and differentiating. (medicalnewstoday.com)
Ineffective hematopoiesis in cases of myelodysplastic syndromes - the role of CD34+ stem and progenitor cells. (bsb-bg.eu)
In approximately 15% of patients, however, myelofibrosis is accompanied by significant extramedullary hematopoiesis, hepatosplenomegaly, and transfusion-dependent anemia, which are manifestations of stem cell failure. (basicmedicalkey.com)

This suggests that in most cases primary CD34+ AML is a progenitor disease where LSCs acquire abnormal self-renewal potential. (ox.ac.uk)
Abnormal mutations in stem cells were found accompanying with the occurrence of MPN. (biomedcentral.com)

Further studies have shown that CD68 is also expressed by a subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells. (biolegend.com)

They give rise to either a common lymphoid progenitor (CLP, a progenitor for all lymphoid cells) or a common myeloid progenitor, CMP, an oligopotent progenitor cell, that gives rise to the myeloid part of the haematopoietic tree. (wikipedia.org)

Accelerated hematopoietic cell apoptosis has been demonstrated in virtually all IBMFS. (medscape.com)

Moreover, Gal-9 expression was increased in response to doxorubicin in various human and murine cancer cell lines. (bvsalud.org)
The cell lines were established by conditional immortalization of primary murine marrow progenitors with an estrogen-regulated E2a/Pbx1-estrogen receptor fusion protein. (nih.gov)

T cell-derived granzyme B (GZMB) activated caspase-6, which subsequently cleaved GSDMC to induce pyroptosis. (bvsalud.org)
Periodic phlebotomies thereafter serve to maintain the red cell mass within the normal range and to induce a state of iron deficiency that prevents an accelerated reexpansion of the red cell mass. (basicmedicalkey.com)
There are other growth factors that only induce specific committed progenitor cells. (wikilectures.eu)

To validate this putative candidate gene and to explore its function, we used CRISPR/Cas9 genome editing to discover that cultured TPM1 -deficient induced pluripotent stem cells enhanced hematopoietic progenitor cell formation. (biomedcentral.com)

These are multipotent cells that reside in the bone marrow niche and have the ability to give rise to all haematopoietic cells, as well as the ability of self renewal. (wikipedia.org)
These are secreted by other haematopoietic cells in the bone marrow or at the site of inflammation as well as epithelial and endothelial cells. (wikipedia.org)
It is arise from hematopoietic stem and progenitor cells in the bone marrow. (medgadget.com)
The etiology of bone marrow failure (BMF) includes defective stem/progenitor cells and/or stroma/accessory cells/growth factors, as well as deficient nonspecific nutrients or, as in the case of acquired aplastic anemia, immune-mediated abnormalities. (medscape.com)
Inherited bone marrow failure syndromes (IBMFS) are usually the result of intrinsic stem cell/progenitor defects. (medscape.com)
Once the mouse neutrophil progenitor had been identified, Zhu and her colleagues were able to track down an equivalent neutrophil progenitor in healthy human bone marrow. (lji.org)
Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)
These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)
After harvesting, the patient is given high dose chemotherapy or radiotherapy and the bone marrow function is reconstituted by infusion of the cells harvested earlier. (justia.com)
The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
CCL23 is predominantly expressed in lung and liver tissue, but is also found in placenta, bone marrow and some cell lines of myeloid origin. (rockland.com)
When blood-forming hematopoietic stem cells that reside in the bone marrow age, the production of blood cells is altered, and the body's ability to regenerate lost cells is impaired. (medicalnewstoday.com)
All these cells, along with blood vessels and the extracellular matrix, form the bone marrow niche . (medicalnewstoday.com)
The bone marrow niche includes cells belonging to the stroma, which includes mesenchymal stromal cells , adipocytes (fat cells), nerve cells, connective tissue cells, and cells involved in bone formation and remodeling. (medicalnewstoday.com)
The endothelial cells that form the lining of these blood vessels are also a major part of the bone marrow niche. (medicalnewstoday.com)
Although aging also influences the bone marrow niche, how these changes in the bone marrow niche influence hematopoietic aging is less studied. (medicalnewstoday.com)
An alternative explanation may be needed for why there was no change in CD8+ cDCs in booreana spleen since these cells are known to derive from common dendritic progenitors in bone marrow. (edu.au)
Myeloproliferative neoplasms (MPN) are a group of blood cancers that boost normal blood cell production in the bone marrow. (biomedcentral.com)
Increased hematopoietic activity of the bone marrow in response to growth factor therapy has been associated with transient positive bone imaging changes. (tarsadia.com)
CD43 expression has also been detected on pluripotent hematopoietic stem cells and myeloid, lymphoid, and NK-cell progenitors in the bone marrow. (bdbiosciences.com)
To overcome this problem, methods of ex vivo treatment ('purging') of autologous marrow must be developed that optimally eradicate AML cells but spare normal hematopoietic stem cells. (arizona.edu)
CFU-Leuk), which we have shown are predictive for hematopoietic engraftment and AML log-kill in rats given syngeneic marrow and/or AML cells incubated ex vivo with selected chemotherapeutic agents, will be used in these dose-response studies. (arizona.edu)
In PV, bone marrow panmyelosis is associated with increased numbers of red blood cells and, in some patients, neutrophilia and/or thrombocytosis. (oncohemakey.com)
The bone marrow is made of many cells as well as fat cells (stromal cells), forming rich connective tissue. (wikilectures.eu)
Patients should not receive Sargramostim until the post marrow infusion ANC is less than 500 cells/mm3. (wikidoc.org)
15]. The percentage of bone common of which are trisomy 8, mono- marrow blast cells for estimation of the Myelodysplastic syndrome (MDS) is somy 7 and 5q- [11]. (who.int)

The modified lines including MYB+/+, MYB+/- and MYB-/- were differentiated into hematopoietic cells in our new established serum-free, cytokine-free culture system with minimal BMP4 exposure for mesoderm induction. (nih.gov)
Cytokine-induced expression of transcriptional regulators including cebpb, Ets2, Bcl3, c-Myc, Jun, and Fosl2 are differentially regulated in Socs3-deficient cells. (nih.gov)
The tight regulation by Socs3 of signal transducer and activator of transcription 3 phosphorylation and gene transcription after cytokine receptor ligation significantly influences the fate of myeloid progenitor cells. (nih.gov)
The reason for the myeloid bias in these diseases is unclear, given the role of JAK2 downstream of numerous cytokine receptors, but could reflect qualitative differences in the consequences of JAK2V617F in the context of different receptors. (oncohemakey.com)

The gene reporter system showed that MYB is specifically expressed during hematopoietic commitment in the earliest primitive blood cells. (nih.gov)
We found that MYB was not required for initial hematopoietic commitment of nascent mesoderm and emergence of primitive, yolk sac-type human hematopoietic progenitors. (nih.gov)

The subject's blood can be processed and used to repopulate the destroyed lymphohematopoietic system of a recipient and may in the future be utilized to repair a variety of non-hematopoietic tissues. (justia.com)
The EasySep™ Rat Custom Positive Selection Kit is designed to isolate any rat cells from fresh splenocytes or single cell suspension of other tissues by positive selection. (stemcell.com)
Another characteristic is that senescent cells that accumulate in body tissues over time cause chronic inflammation through the secretion of proinflammatory cytokines, termed senescence-associated secretory phenotype. (biomedcentral.com)
In vascular medicine, various stem cells and adult progenitors have been highlighted as having a vasoreparative role in ischaemic tissues. (biomedcentral.com)
The evaluation of the most suitable timing of cell delivery as well as the number of cells needed to integrate into resident vasculature and promote revascularisation of specific tissues requires careful optimisation and evaluation. (biomedcentral.com)

The activating effects of the mutation are thought to reflect disruption of the normal autoinhibitory function of the JH2 domain, within which JAK2V617F is found, and have been shown to affect downstream pathways, including STATs (especially STAT5), phosphatidylinositol 3-kinase, and MAP kinase pathways in cell lines and animal models. (oncohemakey.com)

We examined whether CPM is expressed by hematopoietic and stromal cells and could degrade stromal cell-derived factor (SDF)-1a, a potent chemoattractant for hematopoietic stem/progenitor cells (HSPC). (razikurd.ir)

Chronic lymphoid leukaemia and hairy cell leukaemia due to chronic exposure to benzene: Report of three cases. (cdc.gov)

Anatomy55

Organisms13

Diseases15

Chemicals and Drugs93

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes47

Disciplines and Occupations1

Information Science3

Monocytes14

Differentiation27

Macrophages6

Dendritic cells3

Erythroid8

Neutrophils18

Stem and progenitor cell1

Lineage14

Precursors2

Colony-stimul8

Macrophage8

Monocyte1

Platelets5

Immature6

Functionally2

Mice14

Proliferation6

Malignancies5

CD3411

Tumor15

Lymphocytes1

Peripheral8

Differentiate4

HSCs7

Terminally differentiated1

Endothelial progenitor cells1

Leukemia20

Precursor cells1

Unipotent2

Overproduction of mature1

Vitro7

Leukemic4

Subsets3

Clonal2

Mobilization4

Production of granulocytes3

Progression3

Megakaryocytes1

Survival2

Mature granulocytes1

Lineages1

Populations2

Hematopoiesis4

Abnormal2

Fibroblasts1

Common lymphoid1

Apoptosis1

Murine2

Induce3

Pluripotent stem1

Marrow27

Cytokine4

Commitment2

Tissues5

Pathways1

HSPC1

Chronic1