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  • Salmonella
  • These initial studies were conducted using the gram-negative pathogen Salmonella enterica serovar Typhimurium and demonstrated that bacterial growth and drug efficacy could be monitored using this novel, noninvasive technology. (asm.org)
  • bacterial
  • In order to monitor long-term pneumococcal infections noninvasively in living mice, a novel gram-positive lux transposon cassette, Tn 4001 luxABCDE Km r , that allows random integration of lux genes onto the bacterial chromosome was constructed. (asm.org)
  • Infection
  • S. pneumoniae causes (lower respiratory infection) pneumonia and (upper respiratory infections) bronchitis, laryngitis, sinusitis, and otitis media (middle ear infection). (sciencephoto.com)
  • Furthermore, ASC −/− mice were more susceptible than wild-type mice to S. pneumoniae , with impaired secretion of IL-1β and IL-18 into the bronchoalveolar lavage after intranasal infection, suggesting that ASC inflammasomes contribute to the protection of host from infection with PLY-producing S. pneumoniae . (jimmunol.org)
  • If we then decrease MRP2 activity during experimental murine infection with S. pneumoniae , we reduce both neutrophil infiltration and bacteremia, showing that MRP2 coordinates this activity in the lung. (asm.org)
  • pathogen
  • This chapter outlines the up-regulation of genes in response to the competence-stimulating peptide (CSP) , as well as a number of recent discoveries indicating that the coordination of gene expression mediated among neighboring Streptococcus pneumoniae cells by CSP makes important contributions to the interaction of this pathogen with its human host, and thus has significance beyond the acquisition of new genetic information that originally drew attention to the system. (asmscience.org)
  • strains
  • The distribution of the orthologous clusters per genome for the 17 strains was entered into the finite-supragenome model, which predicted that (i) the S. pneumoniae supragenome contains more than 5,000 orthologous clusters and (ii) 99% of the orthologous clusters (∼3,000) that are represented in the S. pneumoniae population at frequencies of ≥0.1 can be identified if 33 representative genomes are sequenced. (asm.org)
  • adults
  • S. pneumoniae is a transient member of the normal flora, colonizing the nasopharynx of 40% of healthy adults and children with no adverse effects. (abcam.com)
  • decrease
  • Caspase-1 activation was partially impaired in NLRP3 −/− macrophages, whereas knockdown and knockout of AIM2 resulted in a clear decrease in caspase-1 activation in response to S. pneumoniae . (jimmunol.org)
  • cells
  • 0.05) higher antimicrobial activity (against A. fumigatus , S. racemosum and S. pneumoniae ) and cytotoxic activities as compared to ethyl acetate extract (IC 50 of alkaline ethyl acetate and ethyl acetate extracts=196 and 400 μg/well against colon carcinoma cells and 164 and 397 μg/well against breast carcinoma cells, respectively). (alliedacademies.org)
  • RESULTS
  • These results suggest that ASC inflammasomes, including AIM2 and NLRP3, are critical for caspase-1 activation induced by S. pneumoniae . (jimmunol.org)
  • highly
  • S. pneumoniae D39 was transformed with plasmid pAUL-A Tn 4001 luxABCDE Km r , and a number of highly bioluminescent colonies were recovered. (asm.org)
  • study
  • Our study revealed that apoptosis-associated specklike protein containing a caspase recruitment domain (ASC), an adaptor protein for inflammasome receptors such as nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) and absent in melanoma 2 (AIM2), is essentially required for the induction of caspase-1 activation by S. pneumoniae . (jimmunol.org)
  • This study supports the hypothesis that spontaneous, nonreverting mutations in cpsE serve as a form of adaptive mutation by providing a selective advantage to S. pneumoniae in niches where expression of capsule is detrimental. (asm.org)