There are some strategies to suppress the GvHD after transplantation or to enhance GvL but none of them provide an ideal solution to this problem. (wikipedia.org)
Other strategies currently clinically used for suppressing GvHD and enhancing GvL are for example optimization of transplant condition or donor lymphocyte infusion (DLI) after transplantation. (wikipedia.org)
NK cell allo-reactivity could also be utilized in additional scenarios besides hematopoietic stem cell transplantation (HSCT) with studies in malignant glioma and neuroblastoma individuals demonstrating that NK cell infusions are safe and partially effective (12, 13). (mycareerpeer.com)
Hematopoietic stem cell (HSC) gene therapy is currently performed on CD34+ hematopoietic stem and progenitor cells containing less than 1% true HSCs and requiring a highly specialized infrastructure for cell manufacturing and transplantation. (bvsalud.org)
This phase I trial assessed the safety, efficacy, and immunologic responses to minor histocompatibility antigens following nonmyeloablative allogeneic hematopoietic cell transplantation as treatment for metastatic renal cell carcinoma. (aacrjournals.org)
Eight patients received conditioning with fludarabine and low-dose total body irradiation followed by hematopoietic cell transplantation from an HLA-matched sibling donor. (aacrjournals.org)
Treatment of metastatic renal cell carcinoma with allogeneic hematopoietic cell transplantation after nonmyeloablative conditioning with fludarabine/total body irradiation is feasible and may induce tumor regression or stabilization in some patients. (aacrjournals.org)
Graft-versus-host disease (GVHD) remains a significant complication in patients undergoing allogeneic stem cell transplantation (SCT) using a reduced intensity conditioning regimen. (qub.ac.uk)
This protocol is part of the Canadian National Transplant Research Program (CNTRP), an integrated national coalition of investigators formed to address challenges with solid organ transplantation and hematopoietic stem cell transplantation. (biomedcentral.com)
The vision of CNTRP is to transform the overall effectiveness of transplantation in Canada by increasing transplant numbers and improving long-term graft and patient survival through collaborative multi-disciplinary research. (biomedcentral.com)
The protocol described in this manuscript is part of project two (increasing solid organ and hematopoietic cell donation in Canada) and details a study that aims to develop and test a theory-based, multi-faceted knowledge translation intervention implemented at the hospital level to increase the number of donation after cardiocirculatory death organ donors for transplantation across Canada. (biomedcentral.com)
Universal Engraftment after Allogeneic Hematopoietic Cell Transplantation Using Cryopreserved CD34-Selected Grafts. (cornell.edu)
Evidence indicates that residual cancer cells contaminate autologous grafts and may contribute to early relapses after autologous stem cell transplantation. (elsevierpure.com)
Successful outcome following hematopoietic stem cell transplantation (HSCT) is ultimately determined by the ability to achieve sustained engraftment and immune reconstitution, control of graft-versus-host disease (GVHD), and in patients with hematological malignancy the complete eradication of abnormal or malignant cells. (johnshopkins.edu)
The ability to correct genetic hematopoietic diseases by gene therapy has a number of advantages over current therapies that involve allogeneic hematopoietic stem cell transplantation (HSCT). (nih.gov)
Cytomegalovirus reactivation is still a critical concern following allogeneic hematopoietic cell transplantation, and cellular immune reconstitution of cytomegalovirus-specific cytotoxic T-cells is necessary for the long-term control of cytomegalovirus reactivation after allogeneic hematopoietic cell transplantation. (nature.com)
These methods and results can help us to better understand immune reconstitution following hematopoietic cell transplantation, leading to future studies on the clinical application of adoptive T-cell therapies. (nature.com)
After undergoing an allogeneic haematopoietic stem cell transplantation (alloHCT), patients need intensive physiological and psychological rehabilitation. (mdpi.com)
The influence of methotrexate-related transporter and metabolizing enzyme gene polymorphisms on peri-engraftment syndrome and graft-versus-host disease after haplo-hematopoietic stem cell transplantation in pediatric patients with malignant hematological diseases. (cdc.gov)
Approximately one-third of children with acute myeloid leukemia (AML) experience disease relapse despite risk-adapted intensive multiagent chemotherapy with or without hematopoietic cell transplantation (HCT) [ 1 ]. (biomedcentral.com)
GvHD17
It is closely linked with graft-versus-host disease (GvHD), as the underlying principle of alloimmunity is the same. (wikipedia.org)
Whereas graft-versus-host-disease (GvHD) has a negative impact on the host, GvL is beneficial for patients with hematopeietic malignancies. (wikipedia.org)
The possibilities of GvL effect in the treatment of hematopoietic malignancies are limited by GvHD. (wikipedia.org)
G-CSF can help to enhance GvL effect and suppress GvHD by reducing levels of LPS and TNF-α. (wikipedia.org)
providing graft vs. GSK1059865 leukemia (GvL) effect while suppressing graft vs. sponsor disease (GvHD) particularly when a KIR ligand mismatch in the donor to sponsor direction was observed. (mycareerpeer.com)
[ 1 ] The number continues to increase by 10-20% annually, and reductions in organ damage, infection, and severe, acute graft versus host disease (GVHD) seem to be contributing to improved outcomes. (medscape.com)
Although T-cell depletion (TCD) reduces the risk of GVHD after a myeloablative conditioning regimen, it is associated with an increased risk of graft failure. (qub.ac.uk)
All but one patient demonstrated durable engraftment and no patient receiving a TCD product developed severe GVHD. (qub.ac.uk)
Graft-versus-host disease ( GvHD ) is a syndrome , characterized by inflammation in different organs. (wikipedia.org)
GvHD can also occur after a blood transfusion , known as Transfusion-associated graft-versus-host disease or TA-GvHD if the blood products used have not been gamma irradiated or treated with an approved leukocyte reduction system. (wikipedia.org)
A new NSG TM variant strain harboring null mutations in mouse MHC I and MHC II (NOD.Cg- Prkdc scid H2-Ab1 em1Mvw H2-K1 tm1Bpe H2-D1 tm1Bpe Il2rg tm1Wjl /SzJ, JAX Stock# 025216 ) has significantly delayed and diminished GvHD following human PBMC engraftment (Brehm et al. (jax.org)
Areas of interest include the role of the microbiota (the trillions of bacteria living in and on our bodies), nutrition, and exercise in modulating HCT outcomes such as graft-versus-host disease (GVHD) and infections. (dukecancerinstitute.org)
Solid tumors, autoimmune diseases, graft versus host disease (GvHD), genetic diseases and neurodegenerative diseases are investigated using many types of autologous and allogeneic cells with rapidly evolving cell engineering and editing approaches. (bdbiosciences.com)
Second, the use of the patient's own bone marrow cells for gene replacement does not result in graft-versus-host disease (GVHD), a major complication of current allogeneic transplant. (nih.gov)
Low-dose post-transplant cyclophosphamide (PTCy) in conjunction with anti-thymocyte globulin (ATG) appears as a potentially effective graft-versus-host disease (GVHD) prevention strategy in haploidentical hematopoietic cell transplant (haplo-HCT). (biomedcentral.com)
Recently, we established a regimen using low-dose PTCy in conjunction with standard-dose ATG in order to lower the risk of GVHD without compromising engraftment and disease relapse. (biomedcentral.com)
In addition, low-dose PTCy could augment the protective effect of ATG on GVHD both in mouse and human sets while ensuring high rates of engraftment and keeping disease control [ 18 ]. (biomedcentral.com)
Disease16
Four patients developed acute, grade 2 to 3, graft- versus -host disease and four patients developed extensive chronic graft- versus -host disease. (aacrjournals.org)
Not to be confused with Host-versus-graft disease . (wikipedia.org)
Mouse colon impacted by acute graft-versus-host disease. (wikipedia.org)
Micrographs of grades of skin graft-versus-host disease: Ranging from grade I GvHR (with minimal vacuolization in the epidermis) to grade II GvHR (with vacuolization and dyskeratotic bodies) to grade III GvHR (with sub epidermal cleft formation) and finally to grade IV GvHR (with separation of the dermis from the epidermis). (wikipedia.org)
In the clinical setting, graft-versus-host disease is divided into acute and chronic forms, and scored or graded on the basis of the tissue affected and the severity of the reaction. (wikipedia.org)
In the classical sense, acute graft-versus-host disease is characterized by selective damage to the liver , skin (rash), mucosa , and the gastrointestinal tract . (wikipedia.org)
Newer research indicates that other graft-versus-host disease target organs include the immune system (the hematopoietic system , e.g., the bone marrow and the thymus ) itself, and the lungs in the form of immune-mediated pneumonitis . (wikipedia.org)
[6] Chronic graft-versus-host disease also attacks the above organs, but over its long-term course can also cause damage to the connective tissue and exocrine glands . (wikipedia.org)
3. Effect of upregulated TLR2 expression from G-CSF-mobilized donor grafts on acute graft-versus-host disease. (kseh.kr)
10. Granulocyte colony-stimulating factor-induced immature myeloid cells inhibit acute graft-versus-host disease lethality through an indoleamine dioxygenase-independent mechanism. (kseh.kr)
Interestingly, the authors developed and used an Epstein Barr Virus-specific human T cell population to prevent complications with Graft Versus Host Disease (Dao et al. (jax.org)
Building off experience in hematopoietic stem cell transplant and exploiting advances in genetic engineering, genome editing, immunology, immunotherapy, stem cell biology, understanding disease mechanisms and cell therapy manufacturing, considerable progress has been made with hundreds of clinical trials underway. (bdbiosciences.com)
For this research we are using the genetic disease, canine leukocyte adhesion deficiency (CLAD), as a model for applying advances in understanding of stem cell biology to enhance gene transfer into the hematopoietic stem cells. (nih.gov)
To date ex vivo gene therapy in hematopoietic disease has only been successful in diseases in which the transgene carries a selective advantage. (nih.gov)
These results represent the first successful use of a foamy virus (FV) vector to treat a genetic disease, and they suggest that FV vectors will be effective in treating human hematopoietic diseases. (nih.gov)
While UL138 is pro-latency, restricting virus replication in CD34+ hematopoietic progenitor cells (HPCs), UL135 overcomes this restriction and is required for reactivation. (bvsalud.org)
Fanconi anemia (FA) is an inherited disorder affecting DNA repair in hematopoietic (blood) stem and progenitor cells (HSPCs) from the bone marrow. (fredhutch.org)
Here, we demonstrate that ex vivo treatment with an oncolytic poxvirus called myxoma virus results in specific elimination of human myeloma cells by inducing rapid cellular apoptosis while fully sparing normal hematopoietic stem and progenitor cells. (elsevierpure.com)
The specificity of this elimination is based on strong binding of the virus to myeloma cells coupled with an inability of the virus to bind or infect CD34 + hematopoietic stem and progenitor cells. (elsevierpure.com)
B lood cell differentiation begins with multipotent hematopoietic progenitor cells (HPCs), which are located in the marrow spaces of the bone. (nationalacademies.org)
HSCT2
For some forms of hematopoietic malignancies, for example acute myeloid leukemia (AML), the essential cells during HSCT are, beside the donor's T cells, the NK cells, which interact with KIR receptors. (wikipedia.org)
The biological questions with MonoMAC center around whether the individual leukocyte compartments that are lost in MonoMAC (B-lymphocytes, NK cells, and monocytes) will be constituted with HSCT, and whether the residual recipient T-lymphocytes will represent an impediment to engraftment. (nih.gov)
Transplant5
NK cells are within the first cells to repopulate host's bone marrow which means they play important role in the transplant engraftment. (wikipedia.org)
To improve isolation and modification of HSPCs as well as their engraftment in the transplant recipients, the authors modified recommendations from the international FA Gene Therapy Working Group. (fredhutch.org)
T-replete mismatched cord blood transplant (TRCB) offers an augmented graft versus leukemia (GVL) effect mediated by alloreactive donor T cells and thereby presents a potential for long term remission and cure in high risk and relapsed refractory AML in children. (ebmt.org)
Infl uenza is a major cause of illness and death in patients zanamivir, use of concomitant antimicrobial therapy, and with hematologic malignancy and in hematopoietic cell intravenous immunoglobulin was determined by the treat- transplant (HCT) recipients. (cdc.gov)
Third, use of autologous hematopoietic stem cells would be expected to enhance immune reconstitution after transplant. (nih.gov)
Myeloid2
Patients were monitored for donor engraftment of myeloid and lymphoid cells, for clinical response by serial imaging, and for immunologic response by in vitro isolation of donor-derived CD8 + CTLs recognizing recipient minor histocompatibility (H) antigens. (aacrjournals.org)
All patients but one in ATG cohort achieved myeloid engraftment by day 30 post-HCT. (biomedcentral.com)
This was followed by neutrophil engraftment on day +19. (ebmt.org)
Stem cell4
We hope that this study will demonstrate the ability to not only isolate more of this limited stem cell pool from FA patients, but will also render these fewer cells more fit for engraftment. (fredhutch.org)
They were able to suppress tumor growth in lung, sarcoma, bladder, breast cancer PDX, and a breast cancer cell line when co-engrafted in CD34+ hematopoietic stem cell (HSC) humanized NSG TM mice (Wang et al. (jax.org)
and 3) studies in the canine model have been predictive of success in humans in the field of hematopoietic stem cell biology. (nih.gov)
Dependence of Human Stem Cell Engraftment and Repopulation of NOD/SCID Mice on CXCR4. (wanfangdata.com.cn)
Minor histocom1
The biology of GvT response is still not fully understood but it is probable that the reaction with polymorphic minor histocompatibility antigens expressed either specifically on hematopoietic cells or more widely on a number of tissue cells or tumor-associated antigens is involved. (wikipedia.org)
Donor T cells1
The graft contains donor T cells (T lymphocytes) that can be beneficial for the recipient by eliminating residual malignant cells. (wikipedia.org)
Immune6
In vitro cell culture and animal studies have demonstrated that mesenchymal stromal cells (MSCs) have the capacity to modify immune responses and to enhance tissue repair. (bmj.com)
White blood cells of the donor's immune system which remain within the donated tissue (the graft) recognize the recipient (the host) as foreign (non-self). (wikipedia.org)
The engraftment of CD34+ haematopoietic stem cells in the triple knockout NCG mouse allows the reconstitution of a complete human immune system, with mature T cells, NK cells and macrophages. (transcurebioservices.com)
The human immune system could also be enhanced by the expression of human cytokines that will improve the immune system development. (transcurebioservices.com)
We describe here how we developed, validated and used a unique IMC panel to analyze the phenotype and the function of the infiltrating human immune cells, the remaining mouse immune macrophages and the developed tumor structure, at once, in HM grafted with 4 different tumor cell lines. (transcurebioservices.com)
NK cells contribute to cancer immune surveillance not only by their direct natural cytotoxicity which is triggered rapidly upon stimulation through germline-encoded cell surface receptors, but also by modulating T-cell mediated antitumor immune responses through maintaining the quality of dendritic cells and enhancing the presentation of tumor antigens. (frontiersin.org)
CD343
We have previously identified the CD34+CD90+ subset to be exclusively responsible for short- and long-term engraftment. (bvsalud.org)
iii) absence of several haematopoietic and endothelial markers (ie, CD45, CD34, CD11b or CD14, CD79 or CD19 and HLA-DR in human MSCs). (bmj.com)
It could also be due to supporting cells in the graft, which are normally excluded when CD34 + cells are directly enriched. (fredhutch.org)
Cell4
CD70 CAR-T cells were modified to express receptors for IL-8 to enhance intratumoral T cell migration as expression of this chemokine is increased in tumor tissue and can be stimulated by radiation. (jax.org)
This ex vivo rabbit-specific oncolytic poxvirus called myxoma virus treatment also effectively inhibits systemic in vivo engraftment of human myeloma cells into immunodeficient mice and results in efficient elimination of primary CD138 + myeloma cells contaminating patient hematopoietic cell products. (elsevierpure.com)
We have generated a high affinity and selective humanized monoclonal IgG4 antibody, TSR-033, which binds human LAG-3 and serves as a functional antagonist, enhancing in vitro T-cell activation both in mixed lymphocyte reactions and staphylococcal enterotoxin B-driven stimulation assays. (aacrjournals.org)
Furthermore, similar to T cells, specific recognition and elimination of cancer cells by NK cells can be markedly enhanced through expression of chimeric antigen receptors (CARs), which provides an opportunity to generate NK-cell therapeutics of defined specificity for cancer immunotherapy. (frontiersin.org)
Cells10
The direct involvement of allo-reactive NK GSK1059865 cells in inducing anti-tumor effect in hematopoietic transplants was first shown in 2002 (8). (mycareerpeer.com)
Mesenchymal stromal cells (MSCs) are cells of non-haematopoietic origin, with the capacity to differentiate into multiple lineages of the mesenchyme, that is, chondrocytes, osteoblasts and adipocytes. (bmj.com)
2 MSCs were first described in the bone marrow where they constitute a small fraction of cells (0.001%-0.01%) that closely interact with haematopoietic cells to support haematopoiesis and skeletal homeostasis. (bmj.com)
CD8 + CTL-recognizing minor H antigens on tumor cells can be isolated posttransplant and could contribute to the graft- versus -tumor effect. (aacrjournals.org)
As explained by Dr. Adair, "our previous work and in research done by others in the field, there has been a clear need to correct the FA defect to elicit engraftment of FA cells. (fredhutch.org)
We conclude that ex vivo myxoma treatment represents a safe and effective method to selectively eliminate myeloma cells from hematopoietic autografts before reinfusion. (elsevierpure.com)
The long-term objective is to develop strategies that will allow levels of expression of CD18 in hematopoietic cells of children with leukocyte adhesion deficiency (LAD) that are sufficient to reverse the clinical phenotype. (nih.gov)
Bone2
eg, bone, bone marrow, and skin grafts) Genetically identical (syngeneic [between monozygotic twins]) donor tissue (isografts) Genetically. (msdmanuals.com)
Engraftment of donor-type MSC-derived osteoblasts was shown using bone specimens and microsatellite DNA marker analysis. (stemcellslab.net)
Reaction1
GvL is a part of the reaction of the graft against the host. (wikipedia.org)
Chimerism1
All patients achieved initial mixed hematopoietic chimerism with two patients rejecting their graft and recovering host hematopoiesis. (aacrjournals.org)
Leukemia1
Graft-versus-leukemia (GvL) is a specific type of GvT effect. (wikipedia.org)
Vivo1
Our novel HSC-targeted viral vectors have the potential to significantly enhance the feasibility of ex vivo gene therapy and pave the way for future in vivo applications. (bvsalud.org)
Syndrome1
B) Engraftment syndrome and consider administration of steroids. (ebmt.org)
Tissue1
Some previous studies also demonstrated that MSCs act as gene therapy delivery vehicles and attenuate lung injury through enhancing the target gene expression in specific damaged tissue sites in the lungs [ 14 , 15 ]. (hindawi.com)
Patients1
When asked about future directions, Dr. Adair concluded: "Of course we are interested to see whether the enhanced engraftment observed in mice plays out in our patients. (fredhutch.org)
Cellular1
In combination with cellular therapy, they offer the potential for a more customized and less harmful treatment routine as these specialized drugs have been shown to not only reduce tumor burden but also enhance the function of cellular treatments. (mycareerpeer.com)
Fludarabine1
We have therefore examined whether TCD compromises engraftment using a fludarabine-based conditioning regimen. (qub.ac.uk)
MSCs1
Recent studies have demonstrated that the homing capability is improved, and the therapeutic effect is increased by enhancing the expression of CXCR4 gene in MSCs [ 19 - 21 ]. (hindawi.com)
Inhibit1
HHV-6A was found to both enhance expression and inhibit degradation of amyloid precursor protein in vitro. (hhv-6foundation.org)
CXCR42
Transfection with CXCR4 enhanced the quantity of transplanted HUMSCs in the radiation-induced injured lung tissues. (hindawi.com)
These finding suggests that CXCR4-overexpressing HUMSCs enhance the protection against RILI and may be a promising strategy for RILI treatment. (hindawi.com)