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  • Cisternae
  • C.J.M. Beckers and J.E. Rothman , Transport between Golgi Cisternae. (elsevier.com)
  • T. Serafini and J.E. Rothman , Purification of Golgi Cisternae-Derived COP-Coated Vesicles. (elsevier.com)
  • Together with its adaptor p47, on the other hand, it regulates several membrane fusion events, including reassembly of Golgi cisternae after mitosis. (rupress.org)
  • Here, we show that p97-p47-mediated reassembly of Golgi cisternae requires ubiquitin, but is not dependent on proteasome-mediated proteolysis. (rupress.org)
  • The best characterized is reassembly of Golgi cisternae from dispersed components at the end of mitosis. (rupress.org)
  • This in turn suggests (but does not prove) that ubiquitin is involved in p97-mediated reformation of Golgi cisternae and raises questions as to the role of ubiquitin in the process and whether this entails proteasome-dependent proteolysis. (rupress.org)
  • Golgi reassembly stacking protein 65 (GRASP65), an NEM-sensitive membrane-bound component, is required for the stacking process.Temporal analysis suggests that p115 plays a transient role in stacking that may be upstream of GRASP65-mediated stacking.These results implicate p115 and its receptors in the initial alignment and docking of single cisternae that may be an important prerequisite for stack formation. (nih.gov)
  • During telophase, Golgi cisternae are regenerated and stacked from a heterogeneous population of tubulovesicular clusters. (nih.gov)
  • In brief, coat protein complex II (COPII)-coated vesicles bud off from the endoplasmic reticulum (ER) and coalesce with early Golgi cisternae, which ensure protein glycosylation. (biomedcentral.com)
  • Glycosylation
  • Glycosylation is a major form of post-translational modification and plays various important roles in organisms by modifying proteins or lipids, which generates functional variability and can increase their stability. (iospress.com)
  • Glycosylation is a post-translational modification where sugars (monosaccharides) or sugar chains (oligosaccharides) are covalently bound to proteins or lipids. (iospress.com)
  • receptors
  • Functional disruption of individual components of a putative lateral targeting patch (e.g., microtubules, the exocyst, and soluble N -ethylmaleimide-sensitive factor attachment protein receptors) did not inhibit cell-cell adhesion or colocalization of the other components with E-cadherin, but each blocked AQP3 delivery to forming cell-cell contacts. (rupress.org)
  • Previously, we reported that activation of G protein-coupled receptors (GPCR) in 1321N1 human astrocytoma cells elicits a rapid release of ATP that is partially dependent on a G q /phophospholipase C (PLC)/Ca 2+ mobilization signaling cascade. (physiology.org)
  • adaptor
  • Linker for activation of T-cells (LAT) is a crucial adaptor protein in T-cell signaling, and its phosphorylation and palmitoylation are essential for its localization and function. (readbyqxmd.com)
  • Cytosolic adaptor proteins (APs), which recognize the cytosolic domains of proteins that span the SG membrane, have been shown to play essential roles in the assembly of functional SGs. (frontiersin.org)
  • Adaptor protein 1A (AP-1A) is known to interact with specific motifs in its cargo proteins and with the clathrin heavy chain, contributing to the formation of a clathrin coat. (frontiersin.org)
  • mammalian
  • Although many of the major molecular players involved in ER-Golgi trafficking in mammalian and yeast ( Saccharomyces cerevisiae ) cells have homologs in higher plants, the narrow interface (less than 500 nm) between the Golgi and the ER, together with the motility factor, makes the identification of the transport vectors responsible for bidirectional traffic between these two organelles much more difficult. (plantphysiol.org)
  • We studied the subcellular localization of MACF1b proteins in mammalian cell lines. (biologists.org)
  • We tested elements of this hypothesis by expressing human alpha-synuclein in mammalian kidney and neuroendocrine cells and assessing ER-to-Golgi transport. (umt.edu)
  • yeast
  • M.E. Groesch, G. Rossi, and S. Ferro-Novick , Reconstitution of ER to Golgi Transport in Yeast: Use of an in Vitro Assay to Characterize Secretory Mutants and Functional Transport Vesicles. (elsevier.com)
  • L. Hicke, T. Yoshihisa, and R. Schekman , Purification of Yeast Sec23 Protein by Complementation of Mutant Cell Lysates Deficient in ER to Golgi Transport. (elsevier.com)
  • ENGLISH ABSTRACT: The budding yeast Saccharomyces cerevisiae has been successfully utilized in several industrial sectors and has over the last decade emerged as a promising host for the production of valuable heterologous proteins. (sun.ac.za)
  • Ballensiefen W, Ossipov D, Schmitt HD (1998) Recycling of the yeast v-SNARE Sec22p involves COPI-proteins and the ER transmembrane proteins Ufe1 p and Sec20p. (springer.com)
  • maturation
  • The typical cause of lipofuscin formation is the impaired degradation of mitochondria by lysosomal degradative enzymes, and, consistent with this, we found that proper post-Golgi maturation of cathepsin D was impaired in D12-deficient cells. (genes2cognition.org)
  • SGs are formed at the trans -Golgi network and must undergo a maturation process to become responsive to secretagogues. (frontiersin.org)
  • au18 and au13 contain mutations in nsfb , which encodes a protein that is required for the maturation of melanosomes in zebrafish RPE. (arvojournals.org)
  • trans
  • S. Tooze and W. Huttner , Cell-Free Formation of Immature Secretory Granules and Constitutive Secretory Vesicles from the Trans-Golgi Network. (elsevier.com)
  • S.G. Miller and H.H. Moore , Movement from Trans-Golgi Network to Cell Surface in Semi-intact Cells. (elsevier.com)
  • Exocytosis of trans-Golgi network (TGN)-derived dense-core granules was substantially inhibited by expression of α-SNAP(L294A). (jneurosci.org)
  • In contrast, dense-core granule exocytosis in adrenal chromaffin cells involves a one-shot fusion event of a granule derived from the trans-Golgi network (TGN) ( Winkler, 1977 ), which in the absence of its released content cannot effectively resequester catecholamine ( Phillips, 1982 ). (jneurosci.org)
  • gene
  • The SGR2 (At1g31480) gene encodes a phospholipase A1-like protein localized to the vacuolar membrane, although its biochemical function is not yet known. (pnas.org)
  • STX10 (Syntaxin 10) is a Protein Coding gene. (genecards.org)
  • A. Tavitian and A. Zahraoui , Rab Proteins and Gene Family in Animals. (elsevier.com)
  • STX18 (Syntaxin 18) is a Protein Coding gene. (genecards.org)
  • Whole-genome sequencing and SNP mapping identified a nonsense mutation in the N-ethylmaleimide-sensitive factor b ( nsfb ) gene in au18 mutants. (arvojournals.org)
  • First, we put the glaA gene encoding for glucoamylase (GlaA), the most abundant secreted protein of A. niger , under control of the tunable Tet-on system. (biomedcentral.com)
  • By inducing glaA expression in a fluorescently tagged v-SNARE reporter strain expressing GFP-SncA, we could demonstrate that the amount of post-Golgi carriers indeed depends on and correlates with glaA gene expression. (biomedcentral.com)
  • transport
  • G. Cellular biological analyses on fibroblasts from subject 3 using two different model cargo proteins did not reveal defects in protein transport. (deepdyve.com)
  • H.W. Davidson and W.E. Balch , Use of Two-Stage Incubations to Define Sequential Intermediates in ER to Golgi Transport. (elsevier.com)
  • M.F. Rexach and R. Schekman , Use of sec Mutants to Define Intermediates in Protein Transport from ER. (elsevier.com)
  • Participates in docking and fusion stage of ER to cis-Golgi transport. (uniprot.org)
  • The review begins with an article by Federica Brandizzi that provides the necessary molecular background on coat protein complexes in relation to the so-called secretory units model for ER-Golgi transport in highly vacuolated plant cells. (plantphysiol.org)
  • Biochemical and molecular analysis of protein sorting into vesicles, transport and docking of vesicles with specialized membrane domains. (stanford.edu)
  • This suggests that a higher dosage of Sed5p may improve ER-to- Golgi protein transport to such an extent that the UPR response diminished. (sun.ac.za)
  • The secretory delay occurred at low expression levels and was not accompanied by insoluble alpha-synuclein aggregates or mistargeting of transport machinery, suggesting a direct action of soluble alpha-synuclein on trafficking proteins. (umt.edu)
  • factor
  • At least two pathways are involved, one catalyzed by p97, the other by another AAA-ATPase, N-ethylmaleimide-sensitive factor (NSF) ( Shorter and Warren, 2002 ). (rupress.org)
  • MACF1 (microtubule actin crosslinking factor), also called ACF7 (actin crosslinking family 7) is a cytoskeletal linker protein that can associate with both actin filaments and microtubules. (biologists.org)
  • The Golgi-localized, γ-ear containing, ADP-ribosylation factor binding (GGA) family of APs serve a similar role. (frontiersin.org)
  • Soluble GRASP65 may then inhibit the stacking process by interacting with a Golgi membrane-bound factor. (nih.gov)
  • highly conserved
  • The identification of a number of highly conserved proteins that are important for membrane trafficking and fusion, combined with the development of new techniques for monitoring exocytosis have permitted models of the molecular events leading up to the calcium-dependent fusion of synaptic vesicles to be proposed. (rupress.org)
  • fragmentation
  • Pathological Golgi fragmentation represents a constant pre-clinical feature of many neurodegenerative diseases including amyotrophic lateral sclerosis (ALS) but its molecular mechanisms remain hitherto unclear. (biomedcentral.com)
  • We conclude that Stathmin-triggered microtubule destabilization mediates Golgi fragmentation in mutant SOD1-linked ALS and potentially also in related motor neuron diseases. (biomedcentral.com)
  • While the molecular mechanisms of Golgi fragmentation in ALS remain largely to be deciphered, at least two mechanisms can been proposed. (biomedcentral.com)
  • The role of microtubules in mutant SOD1-linked Golgi fragmentation remained however unclear. (biomedcentral.com)
  • pathways
  • p97/Cdc48 (in mammals also called VCP for valosin-containing protein) is an essential AAA-ATPase that participates in a wide variety of cellular pathways using distinct adapters and other cofactors ( Woodman, 2003 ). (rupress.org)
  • Granules
  • However, suppression of D12 expression caused rapid appearance of lipofuscin granules, accompanied by apoptotic cell death without the apparent activation of the unfolded protein response. (genes2cognition.org)
  • Sed5p
  • When Sed5p, which led to an improved secretion phenotype for Sf-Cel3A, was overexpressed in conjunction with the aforementioned reporter protein, the UPR activation was notably diminished. (sun.ac.za)
  • Bracher A, Weissenhorn W (2002) Structural basis for the Golgi membrane recruitment of Sly1pby Sed5p. (springer.com)
  • structural
  • After proteolytic processing of the amino terminal part of the polyprotein, the three structural proteins, core and the envelope proteins E1 and E2, are formed. (hindawi.com)
  • The pore forming p7 protein (for a recent review, see [ 3 ]) stands between the structural proteins and the nonstructural proteins (NS), NS2, NS3, NS4A, NS4B, NS5A, and NS5B [ 4 - 6 ]. (hindawi.com)
  • Genome replication does not require the presence of the viral structural proteins enabling the establishment of subgenomic replicons that allow the analysis of genome replication in the absence of virion formation. (hindawi.com)
  • Cells
  • Protein-protein interaction between AtVAM3 and AtVTI11 in the endodermal cells was detected immunologically. (pnas.org)
  • S.A. Adam, R. Sterne-Marr, and L. Gerace , Analysis of Nuclear Protein Import Using Digitonin-Permeabilized Cells. (elsevier.com)
  • I. Corthesy-Theulaz and S.R. Pfeffer , Microtubule-Mediated Golgi Capture by Semi-intact CHO Cells. (elsevier.com)
  • The asymmetric distribution of membrane proteins in different cell surface domains is a feature common to many types of polarized cells, including epithelia, neurons, and immune cells ( Yamada and Nelson, 2007 ). (rupress.org)
  • fungal
  • Increased secretory titers for the industrially significant fungal cellulases in the S. cerevisiae protein production host would greatly contribute to the economic feasibility of second generation bioethanol production. (sun.ac.za)
  • The accepted paradigm is that proteins become mainly secreted at the tips of fungal hyphae. (biomedcentral.com)
  • amino
  • Monosaccharides are the minimum unit of glycans, like amino acids and nucleotides in protein or nucleic acids, respectively. (iospress.com)