• Furthermore, we demonstrate the ability of cell-free synthesized OSTs to glycosylate multiple target proteins with varying N -glycosylation acceptor sequons. (biorxiv.org)
  • Current evidence indicates that the STT3A isoform of the OST is primarily responsible for cotranslational glycosylation of nascent glycoproteins as the acceptor sequons exit the luminal face of the translocation channel. (umassmed.edu)
  • It usually acts during translation as the nascent protein is entering the ER, but this cotranslational glycosylation is nevertheless called a posttranslational modification. (wikipedia.org)
  • Scan the nascent protein in order to recognize and bind sequons. (wikipedia.org)
  • Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. (biorxiv.org)
  • Specifically, by using lipid nanodiscs as cellular membrane mimics, we obtained yields of up to 440 µg/mL for the single-subunit OST enzyme, 'Protein glycosylation B' (PglB) from Campylobacter jejuni , as well as for three additional PglB homologs from Campylobacter coli, Campylobacter lari , and Desulfovibrio gigas . (biorxiv.org)
  • Roitsch T, Lehle L. Structural requirement for protein glycosylation. (rusmedserv.com)
  • As a post-doctoral fellow in Bill Lennarz' laboratory, Jerry defined the minimal sequence requirement for N -linked protein glycosylation, the so-called "sequon" [N-X(not P)-S/T]. Jerry continued his studies on N -glycosylation as a junior professor at Johns Hopkins School of Medicine, firmly established the concept of site-specific oligosaccharide microheterogeneity, and demonstrated this is a non-random process that fine-tunes glycoprotein functions. (glycobiology.org)
  • Knowledge of the three-dimensional structures of the carbo-hydrate molecules is indispensable for a full understanding of the molecular processes in which carbohydrates are involved, such as protein glycosylation or protein-carbohydrate interactions. (iucr.org)
  • Glycosylation is classified by the way the carbohydrate chain is linked to the protein. (iucr.org)
  • Protein glycosylation fulfils a variety of roles. (iucr.org)
  • However, the "glycosylation code" that relates these effects to protein sequence remains unsolved. (bocsci.com)
  • is a compilation of such bacterial and archaeal proteins/enzyme that have miscellaneous accessory roles in a given protein glycosylation pathway of a characterized protein glycosyltransferases compiled in ProGT_Main. (proglycprot.org)
  • N-glycans are covalently linked to an asparagine residue in a simple acceptor sequence of proteins, called a sequon. (nih.gov)
  • However, N-glycosylation of platelet proteins has been poorly investigated in CDG. (nih.gov)
  • It was actually Jerry's labs studies of complex glycosylation on the surface of intact cells in culture that led to the discovery of the O-GlcNAc modification of nuclear and cytoplasmic proteins. (glycobiology.org)
  • Asparagine-linked glycosylation of proteins occurs within the lumen of the RER. (umassmed.edu)
  • The STT3B isoform of the OST can modify skipped sequons on unfolded proteins in the lumen of the ER. (umassmed.edu)
  • N-glycosylation plays an important role in the structure and function of membrane and secreted proteins. (bvsalud.org)
  • Recently, along with our collaborators we have demonstrated that CjPglB has a relaxed specificity enabling transfer of diverse polysaccharides, including lipopolysaccharide and capsules, to proteins with appropriate D/E-X-N-Z-S/T consensus sequons. (ukri.org)
  • STT3A is primarily responsible for cotranslational glycosylation of the nascent polypeptide as it enters the lumen of the endoplasmic reticulum whereas STT3B can also mediate posttranslational glycosylation. (wikipedia.org)
  • Influence of acceptor peptides on cotranslational glycosylation of yeast invertase and site-directed mutagenesis around a sequon sequence. (rusmedserv.com)
  • Cotranslational and posttranslational N-glycosylation of polypeptides by distinct mammalian OST isoforms. (umassmed.edu)
  • The presence of a complex N-glycan at Asn58 is consistent with the relatively acidic environment of the Asn58 N-glycosylation sequon, that predicts N-glycosylation by T. brucei oligosaccharyltransferase TbSTT3A with a Man(5)GlcNAc(2) structure destined for processing to a paucimannose and/or complex N-glycan (Izquierdo L, Schulz B, Rodrigues JA et al. (analyteguru.com)
  • The best understood subclass is N -glycosylation, in which the glycans are linked to the N δ 2 atom of an Asn side chain. (iucr.org)
  • The data showed that half of the N-glycosylation sequons changed their distribution of glycans in the S-614G variant. (bvsalud.org)
  • We report atomically detailed replica exchange molecular dynamics simulations in explicit solvent that systematically investigate the impact of glycosylation upon peptides with the central sequon Pro-Asn-Gly/Ala-Thr-Trp/Ala. These simulations suggest that the effects of glycosylation may be quite sensitive to steric crowding by the side chain immediately following the glycosylation site but less sensitive to stacking interactions with the aromatic Trp residue. (bocsci.com)
  • In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions. (bvsalud.org)
  • is a compilation of bacterial and archaeal glycoproteins for which at least one site of glycosylation is validated experimentally using one or multiple methods like, site directed mutagenesis, Edman degradation, mass spectroscopy etc. (proglycprot.org)
  • However, understanding the roles and regulations of site-specific glycosylation events remains a significant challenge due to several technological limitations. (biorxiv.org)
  • The modification of glycosylation sites for the 1918 and SI/06 viruses also caused changes in viral antigenicity based on cross-reactive hemagglutinin inhibition antibody titers with antisera from mice infected with wild-type or glycan mutant viruses. (health-e-nc.org)
  • removal of glycosylation sites from SI/06 HA imposed constraints on the theoretical structure surrounding the glycan receptor binding sites, which in turn led to distinct glycan receptor binding properties. (health-e-nc.org)
  • Moreover, our analysis highlights the significance of left-handed conformations for compact β-hairpins at glycosylation sites. (bocsci.com)
  • These results demonstrate that glycosylation patterns of the 1918 and seasonal H1N1 viruses directly contribute to differences in virulence and are partially responsible for their distinct antigenicity. (health-e-nc.org)
  • Rawitch AB , Pollock G, Yang SX, Taurog A. Thyroid peroxidase glycosylation: the location and nature of the N-linked oligosaccharide units in porcine thyroid peroxidase. (rusmedserv.com)
  • It usually acts during translation as the nascent protein is entering the ER, but this cotranslational glycosylation is nevertheless called a posttranslational modification. (wikipedia.org)
  • Our study shows new elements involved in the regulation of N-glycosylation, a key posttranslational modification that, according to our results, affects folding, secretion, and function, providing new evidence of the pathogenic consequence of an incorrect N-glycosylation of antithrombin. (bvsalud.org)
  • The X-ray structure of the complete PglB protein from C. lari revealed the molecular basis of acceptor sequon recognition and provided insight into the reaction mechanism. (nih.gov)
  • Mutation experiments reflected the role that Lysine residues close to the N-glycosylation sequon have in impairing the efficacy of N-glycosylation. (bvsalud.org)
  • The 2 variants shared a common functional defect, an impaired or null N-glycosylation of Asn224 according to a eukaryotic expression model. (bvsalud.org)
  • Our study demonstrates that TMTC3 regulates O-linked glycosylation and cadherin-mediated adherence, providing insight into its effect on cellular adherence and migration, as well the basis of TMTC3-associated Cobblestone lissencephaly. (xenbase.org)
  • Structural characterization of recombinant soluble rat neuroligin 1: mapping of secondary structure and glycosylation by mass spectrometry. (nih.gov)
  • our results demonstrate that the N-glycosylation sequon in the A2 domain is located in a structural element that is critically required for proper folding and conformation of FVIII. (cusabio.com)
  • Using mass spectrometry, we have established that at least two of three potential sites of N-linked glycosylation are occupied on most molecules of GP73 secreted from cultured hepatoma cells. (nih.gov)
  • is a compilation of bacterial and archaeal glycoproteins for which at least one site of glycosylation is validated experimentally using one or multiple methods like, site directed mutagenesis, Edman degradation, mass spectroscopy etc. (proglycprot.org)
  • [ 202 ] Of the genes identified as significantly prevalent in either clade, six genes within the O- linked flagellin glycosylation biosynthetic locus were of particular interest. (medscape.com)
  • Using genetically engineered cells lacking all four TMTC genes, overexpression of TMTC3 rescued O-linked glycosylation of E-cadherin and cell adherence. (xenbase.org)
  • The potential N-glycosylation sequons in NL1 at Asn 109, Asn 303, Asn 343, and Asn 547 are shown occupied by carbohydrate. (nih.gov)