• The study of protein glycosylation can be regarded as an intricate but very important task, making glycomics one of the most challenging and interesting, albeit under-researched, type of "omics" science. (whiterose.ac.uk)
  • Disorders in protein glycosylation and potential therapy. (wikipedia.org)
  • Protein glycosylation is a common post-translational modification on extracellular proteins. (bvsalud.org)
  • We have been engineering in vivo protein glycosylation through improving landing sites for glycosyltransferases. (ualberta.ca)
  • The lab is also working on a natural protein glycosylation system (O-mannosylation) in bacteria which has is conserved all the way up the evolutionary ladder. (ualberta.ca)
  • We demonstrate GlycoPRIME by constructing 37 putative protein glycosylation pathways, creating 23 unique glycan motifs, 18 of which have not yet been synthesized on proteins. (nsf.gov)
  • To address these challenges, we describe a novel cell-free glycoprotein synthesis (CFGpS) technology that seamlessly integrates protein biosynthesis with asparagine-linked protein glycosylation. (nsf.gov)
  • The biosynthesis of N-linked glycans occurs via 3 major steps: Synthesis of dolichol-linked precursor oligosaccharide En bloc transfer of precursor oligosaccharide to protein Processing of the oligosaccharide Synthesis, en bloc transfer and initial trimming of precursor oligosaccharide occurs in the endoplasmic reticulum (ER). (wikipedia.org)
  • The base-promoted intramolecular cyclization of Ugi-azide adduct has been demonstrated for the synthesis of highly substituted aziridinyl glycoconjugates in one pot. (cdri.res.in)
  • Our notable contributions towards synthesis of biologically relevant oligosaccharides usin one−pot glycosylation, and one−pot deprotection−glycosylation protocols. (cdri.res.in)
  • The synthesis of the pentasaccharide moiety of starfish asterosaponin luidiaquinoside as their p-methoxyphenyl (PMP) glycosides was achieved followed by sequential glycosylation strategy using suitably functionalized thioglycoside donors. (cdri.res.in)
  • After induction glycoconjugates generally appeared later than unglycosylated carrier protein, suggesting that glycosylation was the rate-limiting step for synthesis of conjugate vaccines in E. coli . (biomedcentral.com)
  • Glycoconjugate synthesis, in particular expression of oligosaccharyltransferase PglB, strongly inhibited growth of E. coli cells after induction, making it necessary to separate biomass growth and recombinant protein expression phases. (biomedcentral.com)
  • We are also applying these enzymes to the synthesis of bioactive glycoconjugates for various therapeutic applications. (ualberta.ca)
  • N-linked glycosylation, is the attachment of an oligosaccharide, a carbohydrate consisting of several sugar molecules, sometimes also referred to as glycan, to a nitrogen atom (the amide nitrogen of an asparagine (Asn) residue of a protein), in a process called N-glycosylation, studied in biochemistry. (wikipedia.org)
  • Evaluation of ion mobility for the separation of glycoconjugate isomers due to different types of sialic acid linkage, at the intact glycoprotein, glycopeptide and glycan level. (whiterose.ac.uk)
  • Particularly, we aim to assess the performance of this platform for the separation of isomeric glycoconjugates due to the type of sialic acid linkage, at the intact glycoprotein, glycopeptide and glycan level. (whiterose.ac.uk)
  • Treatment of cells with inhibitors of these enzymes results in the production of glycans or glycoconjugates containing missing or altered glycan chains. (go.jp)
  • The glycosylation machinery is responsible for assembling a diverse repertoire of glycan structures, collectively termed 'glycome', through the synchronized action of a portfolio of glycan-modifying enzymes including glycosyltransferases and glycosidases. (nature.com)
  • Glycosylation defines the adhesive properties of glycoconjugates and it is largely through glycan-protein interactions that cell- cell and cell-pathogen contacts occur, a fact that highlights the importance of glycobiology. (neb.com)
  • read more Glycomics, a Resource that develops technologies for glycomics, the study of glycans, glycoconjugates, and glycan binding proteins. (hstalks.com)
  • Concomitantly with the gastric mucosal swelling the human being gastric glycosylation patterns switch with manifestation of swelling connected sialylated glycans [11 17 18 Importantly swelling has been shown to modulate the manifestation of the glycosyltransferases involved in the biosynthesis of terminal glycan chains [19-21]. (bio-cavagnou.info)
  • Click-conjugation of the alkinyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates. (uni-konstanz.de)
  • This review highlights recent developments in chemical regulation of glycosylation processes with specific targets including: inhibition of (1) N -glycosylation, (2) O -glycosylation, (3) O -linked GlcNAc glycosylation, (4) proteoglycan biosynthesis, (5) glycolipid biosynthesis, and (6) terminal glycosylation. (go.jp)
  • Availability of specific sugars for glycoconjugate biosynthesis. (wikipedia.org)
  • To list therapeutics based on glycans or glycoconjugates and vaccine development, correlating with clinical application. (unl.pt)
  • Eva Nordberg Karlsson works within the fields of enzymology and microbiology, with a focus on production and biomolecular development of carbohydrate-modifying enzymes for hydrolysis (oligo- and monosaccharides) and trans-glycosylation reactions (for glycoconjugates such as surfactants and antioxidants), using the carbohydrate polymers of the biomass as the starting material. (lu.se)
  • Our work includes design of synthetic operons to add biosynthetic pathways for glycosylation into various host organisms. (ualberta.ca)
  • In GlycoPRIME, glycosylation pathways are assembled by mixing-and-matching cell-free synthesized glycosyltransferases that can elaborate a glucose primer installed onto protein targets by an N -glycosyltransferase. (nsf.gov)
  • The global effort is modeled on the Human Genome Project and aims to map the structures and functions of human glycoconjugates, or glycans, oligosaccharides covalently attached to the backbone of proteins. (genos-glyco.com)
  • PMM2-CDG, the most common of a group of disorders of abnormal glycosylation of N-linked oligosaccharides, is divided into three clinical stages: infantile multisystem, late-infantile and childhood ataxia-intellectual disability, and adult stable disability. (beds.ac.uk)
  • In the present work, the potential of traveling wave IM (TWIMS) for the separation of isomeric glycoconjugates was evaluated, using mouse transferrin (mTf) as model glycoprotein. (whiterose.ac.uk)
  • In vivo enzymatic coupling using the general glycosylation pathway of Campylobacter jejuni in recombinant Escherichia coli has been suggested as a simpler method for producing conjugate vaccines. (biomedcentral.com)
  • The new recombinant glycoconjugates in E. coli will be ideal vaccine candidates that can be readily purified and tested. (ukri.org)
  • In this work we utilized the non-natural amino acid p-azidophenylalanine (pAzF) in combination with the chemoselective Staudinger-phosphite reaction to install branched PEG chains to recombinant unglycosylated erythropoietin (EPO) at each single naturally occurring glycosylation site. (uni-konstanz.de)
  • The sLeX motif can be presented on cell surfaces on protein (i.e., glycoprotein) and/or lipid (i.e., glycolipid) scaffolds, and these glycoconjugates (known as "E-selectin ligands") program shear-resistant adhesion to endothelial cells. (cancer.gov)
  • In order to evoke an appropriate immune response, vaccines against these bacteria consist of capsule linked to a protein carrier forming a glycoprotein or glycoconjugate. (ukri.org)
  • We will identify the pertinent sLeX-bearing glycoconjugates among the different types of human leu- kemia cells, measure the ability of such glycoconjugates to engage E-selectin, and determine how expression of Golgi GTs shape creation of sLeX modifications among the different E-selectin ligands. (cancer.gov)
  • The key steps were a global phosphitylation of the unprotected Ser residue to a dimethylphosphite-containing peptide, glycosylation with a glycosyl azide, deprotection and cleavage from the resin of the phosphoramidate-linked glycopeptide by TFA treatment. (chemistryviews.org)
  • A new practical utility for β-stereoselective glycosylation via activation of O-glycosyl trichloroacetimidate donors using N-benzoylglycine/thiourea cooperative catalysis has been demonstrated. (cdri.res.in)
  • However little is known about the molecular mechanisms governing the glycosylation shift that occurs in gastric mucosa in response to illness. (bio-cavagnou.info)
  • However the activation of this glycosylation pathway as well as the living of additional regulatory mechanisms underlying these glycophenotypic changes in the complex context of individual chronic infection haven't been attended to. (bio-cavagnou.info)
  • Glycosylation processes can be chemically regulated by inhibition of glycosyltransferases or glycosidases with natural and synthetic molecules. (go.jp)
  • This information will be integrated with various biochemical approaches including metabolic inhibition of glycosylation and cell surface glycoengineering to custom-modify sLeX display to assess the extent to which sLeX presentation on a specific protein and/or lipid scaffold licenses E-selectin binding among blasts from various subtypes of human acute leukemias, and the impact of the relevant E-selectin receptor/ligand interaction(s) in leukemia cell biology. (cancer.gov)
  • Indeed many capsules of pathogenic bacteria do not have this configuration and therefore CjPglB could not be used to produce glycoconjugate vaccines for protection against these bacteria. (ukri.org)
  • Biological Role of Site-specific O-glycosylation in Cell Adhesion Activity and Phosphorylation of Osteopontin. (fmu.ac.jp)
  • During inflammation, binding of selectins to cell-surface glycoconjugates mediates rolling adhesion of leukocytes on vascular surfaces. (ashpublications.org)
  • Although one typically thinks of carbohydrates as associated with cell growth and viability, glycosylation also has an integral role in many processes leading to cell death. (nature.com)
  • This approach is highly useful for examining the potential functional role(s) of glycans and glycoconjugates in cells or tissues, and in biological processes of health and disease. (go.jp)
  • Finally, the developed methodology was applied to serum samples of mice, to investigate its robustness when analyzing real complex samples.Ion mobility mass spectrometry is a promising analytical technique for the separation of glycoconjugate isomers due to type of sialic acid linkage. (whiterose.ac.uk)
  • One such approach is to produce glycoconjugate vaccines in the genetically tractable bacterium Escherichia coli. (ukri.org)
  • It was found that efficiency of glycosylation but not carrier protein expression was highly susceptible to the physiological state at induction. (biomedcentral.com)
  • These diverse structures are controlled by complex glycosylation processes in cells, which are mediated by various glycosyltransferases and glycosidases. (go.jp)
  • An inexpensive, rapid and flexible method for glycoconjugate vaccine production would enable a more effective response to the emergence of new pathogenic bacterial strains with different capsule structures. (ukri.org)
  • Asparagine must be located appropriately in the three-dimensional structure of the protein (Sugars are polar molecules and thus need to be attached to asparagine located on the surface of the protein and not buried within the protein) Asparagine must be found in the luminal side of the endoplasmic reticulum for N-linked glycosylation to be initiated. (wikipedia.org)
  • Glycosylation plays important roles in cellular function and endows protein therapeutics with beneficial properties. (nsf.gov)
  • The emerging discipline of bacterial glycoengineering has made it possible to produce designer glycans and glycoconjugates for use as vaccines and therapeutics. (nsf.gov)
  • Therefore, we have measured the degree of scrambling upon glycosidic bond cleavage in glycopeptides that differ in the conformational flexibility of their backbone and glycosylation pattern. (bvsalud.org)
  • This research provides novel medically relevant insights in to the regulatory systems underlying modulation of sponsor glycosylation machinery and phenotypic alterations important for life-long illness. (bio-cavagnou.info)
  • To measure the effect of persistent an infection and gastric mucosa irritation over the transcriptional legislation from the enzymes that determine the web host gastric cell's glycophenotype we've examined the glycosylation as well as the glycosyltransferase transcriptomic profile of gastric biopsies from healthful and infected people. (bio-cavagnou.info)
  • Les interactions sucres/protéines (enzymes, lectines) sont au cœur de la communication cellulaire tant extracellulaire (interactions cellule-cellule, cellule-matrice, hôte-pathogène) qu'intracellulaire (GlcNAcylation en tant que modification post-traductionnelle). (univ-nantes.fr)
  • Our research has been focused on the investigation of the structure and function of the enzymes involved in making various glycoconjugates. (ualberta.ca)
  • This method represents the first instance where amino acid derived N-benzoylglycine is used as a catalyst for O-glycosylation under mild reaction conditions at ambient temperature. (cdri.res.in)
  • EPO variants with an alkyne bearing amino acid (Plk) at the N-glycosylation sites 24, 38 and 83 were obtained by amber suppression followed by refolding. (uni-konstanz.de)
  • Starting from shake flask cultivation in standard complex medium a lab-scale fed-batch process was developed for glycoconjugate production. (biomedcentral.com)
  • We are applying synthetic biology and glycoproteomics to address this complex problem in the hope we can uncover fundamental knowledge of how this glycosylation process plays a role in biology. (ualberta.ca)
  • To relate diagnostic methods / technologies for Congenital Diseases of Glycosylation (CDG), cancer and infection. (unl.pt)
  • In this way, the student will update and expand his knowledge in glycobiology and develop critical thinking for application in glycosylation-related diseases. (unl.pt)
  • Comparative Performance of Four Methods for High-throughput Glycosylation Analysis of Immunoglobulin G in Genetic and Epidemiological Research. (mpg.de)
  • As much of its work is supported by research grants, Lauc said that Genos largely functions as a "research institute," but it offers glycosylation analysis services as well using ultra performance liquid chromatography (UPLC) and liquid chromatography-mass spectrometry (LC-MS). As an associate member, Genos will offer analytical services to the initiative for free. (genos-glyco.com)
  • The established methodology could be regarded as starting point in the separation of highly decorated glycoconjugates. (whiterose.ac.uk)
  • Glycosylation is an evolutionary highly successful mechanism because today it is known that almost all membrane and extracellular proteins are glycosylated. (unios.hr)
  • The SJA histochemistry showed that SJA-binding glycoconjugates (SBG) were highly detected in hyperplastic/dysplastic bile ducts (81.3%, 26/32) and neoplastic bile ducts (77.3%, 34/44) but was not detected in normal bile ducts, hepatocytes and the infiltrating cells. (tci-thaijo.org)
  • We report herein the encapsulation of a semisynthetic glycoconjugate (GC) composed of a synthetic tetrasaccharide mimicking theS. (univ-nantes.fr)
  • this gastric glycosylation change characterized by elevated sialylation patterns mementos SabA-mediated connection to human swollen gastric mucosa. (bio-cavagnou.info)
  • Glycosylation of classical death receptors fine-tunes cell death programs. (nature.com)
  • Is there a hallmark 'glycosylation signature' that characterizes the initiation, execution and resolution of cell death programs in physiologic and pathologic settings? (nature.com)
  • In Glycoconjugate Journal, 28 (5 ), p. 332. (mpg.de)
  • In Glycoconjugate Journal, 28 (5 - Special Issue : XXI International Symposium on Glycoconjugates), p. 331. (mpg.de)
  • Glycoconjugate Journal , 12 (3), 219-226. (eur.nl)
  • La glyco-enzymologie au travers de laquelle l'équipe cherche à identifier, à caractériser et à modifier les propriétés d'enzymes impliquées dans le métabolisme des sucres (glycosides hydrolases, sulfatases) pour les utiliser en tant qu'outils synthétiques pour la production de molécules à haute valeur ajoutée. (univ-nantes.fr)
  • However, despite the success of these glycoconjugate vaccines they have major drawbacks in terms of the technical difficulties in purifying the capsule material from bacterial cells and then conjugating the capsule to carrier proteins. (ukri.org)
  • Multifunctional Glycoconjugates for Recruiting Natural Antibodies against Cancer Cells. (univ-grenoble-alpes.fr)
  • The glycoengineering principles to be pioneered in this study could be applied generically to the design of other glycoconjugate and combination vaccines. (ukri.org)