Glycosylase enzyme. Medical search. Frequent questions

Noncovalent SUMO-1 binding activity of thymine DNA glycosylase (TDG) is required for its SUMO-1 modification and colocalization with the promyelocytic leukemia protein. (nih.gov)

Structural and biophysical analysis of interactions between cod and human uracil-DNA N-glycosylase (UNG) and UNG inhibitor (Ugi). (cathdb.info)
Viral DNA synthesis involves a DNA repair enzyme, the uracil-DNA glycosylase D4 linked to the structural protein A20 forming the processivity factor, which in turn binds to E9 forming the complex required for processive DNA synthesis. (biorxiv.org)
One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from uracil-containing dsDNA. (qiagen.com)
E. coli Uracil DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single-stranded or double-stranded DNA. (qiagen.com)
Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. (thermofisher.com)
Diversify your product portfolio with a wide range of select enzymes like ß-agarase for DNA purification, CRISPR/Cas9 for genome editing, or thermolabile cod uracil-DNA glycosylase for PCR contamination control. (varizymes.com)
An enzyme that catalyzes the HYDROLYSIS of the N-glycosidic bond between sugar phosphate backbone and URACIL residue during DNA synthesis. (musc.edu)
Correlated Mutation in the Evolution of Catalysis in Uracil DNA Glycosylase Superfamily. (musc.edu)
A structural determinant in the uracil DNA glycosylase superfamily for the removal of uracil from adenine/uracil base pairs. (musc.edu)
Specificity and catalytic mechanism in family 5 uracil DNA glycosylase. (musc.edu)
New family of deamination repair enzymes in uracil-DNA glycosylase superfamily. (musc.edu)
Evaluation of the role of the vaccinia virus uracil DNA glycosylase and A20 proteins as intrinsic components of the DNA polymerase holoenzyme. (musc.edu)
Insights from xanthine and uracil DNA glycosylase activities of bacterial and human SMUG1: switching SMUG1 to UDG. (musc.edu)
Uracil-DNA glycosylases]. (musc.edu)
A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine. (ox.ac.uk)
Uracil DNA glycosylase (UDG) efficiently removes uracil from DNA in a reaction in which the base is flipped into the enzyme's active site. (ox.ac.uk)

DNA glycosylases from the Fpg/Nei structural superfamily are base excision repair enzymes involved in the removal of a wide variety of mutagen and potentially lethal oxidized purines and pyrimidines. (rcsb.org)

During the transcription of information (its rewriting into RNA) the RNA polymerase enzyme "rides" on the DNA chain, and stops when it finds the break. (sciencedaily.com)
Like a proofreader of a text, RNA polymerase after it is stalled, triggers a cascade of reactions, resulting in the repair enzymes fixing the damaged area. (sciencedaily.com)
The enzymes remove the damaged base forming an AP site which is repaired by AP endonuclease before the nucleotide gap in the DNA strand is filled by DNA polymerase. (news-medical.net)

Here, we report that XRCC1 interacts physically and functionally with hOGG1, the human DNA glycosylase that initiates the repair by BER of the mutagenic oxidized base 8-oxoguanine. (nih.gov)
This interaction leads to a 2- to 3-fold stimulation of the DNA glycosylase activity of hOGG1. (nih.gov)
XRCC1 stimulates the formation of the hOGG1 Schiff-base DNA intermediate without interfering with the endonuclease activity of APE1, the second enzyme in the pathway. (nih.gov)
8-oxo-dGuo expressions as well as protein and mRNA expressions of DNA repair enzyme hOGG1 and antioxidant defenses (CAT, GCLC, GPx, Nrf2, and MnSOD) in nonneoplastic epidermis of control and BCC tissues were also determined. (hindawi.com)
Furthermore, impaired DNA repair capacity was suggested to associate with enhanced susceptibility to cancer and deficiency in DNA repair enzyme, human 8-oxoguanine DNA N-glycosylase 1(hOGG1), a key enzyme responsible for 8-oxo-dGua repair, may also be involved in carcinogenesis [ 11 ]. (hindawi.com)
In human cells, the enzymatic activities initiating DNA base excision repair (BER) of 8-oxoG, U and AP sites are the 8-oxoG DNA glycosylase (hOGG1), the U-DNA glycosylase (UNG) and the major hydrolytic AP endonuclease (APE/HAP1), respectively. (utmb.edu)
To discriminate whether the different rates of repair may be linked to different expression of the initiating enzymes, we have determined the amount of hOGG1, UNG and APE/HAP1 in normal human cell extracts by immunodetection techniques. (utmb.edu)
Addition to reaction mixtures of titrated amounts of purified hOGG1, UNG and APE/HAP1 variably stimulated the in vitro repair replication of 8-oxoG, U and the AP site respectively and the increase was not always proportional to the amount of added enzyme. (utmb.edu)

Recently, we reported that small molecules can act as organocatalysts for the DNA repair enzyme 8-oxoguanine DNA glycosylase 1 (OGG1). (ki.se)
MPG enzyme activity in peripheral blood mononuclear cells from case patients was higher than in control subjects, results opposite that of 8-oxoguanine DNA glycosylase (OGG1) DNA repair enzyme activity. (nih.gov)
enzyme 8-oxoguanine DNA glycosylase. (informationalwebs.com)
Dodson, ML & Lloyd, RS 2001, ' Backbone dynamics of DNA containing 8-oxoguanine: Importance for substrate recognition by base excision repair glycosylases ', Mutation Research - DNA Repair , vol. 487, no. 3-4, pp. 93-108. (elsevierpure.com)

The dense packing allows DNA molecule with a length of about two meters to fit into a microscopic cell nucleus, but it makes significant surfaces of the DNA inaccessible for the repair enzymes -- the proteins that manage the "repair" of damaged DNA regions. (sciencedaily.com)
Five homologues of glycosylase of pyrimidine dimers of bacteriophage T4, a product of the denV gene, were found by comparing using the DELTA-BLAST algorithm with the compost metagenome proteins. (e3s-conferences.org)
Thus, an interesting spectrum of promising proteins, homologues of the repair enzyme, DNA glycosylase of pyrimidine dimers of bacteriophage T4, was found. (e3s-conferences.org)

The results predict that the FPG enzyme should flip out the cytosine base paired with the scissile 8OG, not the target base itself. (elsevierpure.com)
The N123D mutation generates an enzyme that excises cytosine. (ox.ac.uk)
This enzyme may offer a new approach for discriminating between cytosine and 5-methylcytosine. (ox.ac.uk)

This gene encodes an essential enzyme of nucleotide metabolism. (thermofisher.com)

In mismatch repair, the missed errors are later corrected by enzymes which recognize and excise the mismatched base to restore the original sequence. (news-medical.net)
My current research interests focus on a group of enzymes involved in DNA repair. (salford.ac.uk)
Furthermore, the role of DNA thermodynamic "signatures" in lesion recognition by DNA repair enzymes, such as DNA glycosylases, is poorly understood, again because such enzymes catalyze the repair of these lesions, precluding their biochemical/structural study in corresponding protein/DNA complexes. (hhs.gov)
N-methylpurine DNA glycosylase and OGG1 DNA repair activities: opposite associations with lung cancer risk. (nih.gov)
To examine whether inter-individual variations in DNA repair activity of N-methylpurine DNA glycosylase (MPG) are associated with lung cancer, we conducted a blinded, population-based, case-control study with 100 lung cancer case patients and 100 matched control subjects and analyzed the data with conditional logistic regression. (nih.gov)
Examples of this mechanism are the base excision repair glycosylases and the restriction enzyme methylases. (elsevierpure.com)

In June 2022, we were first to report cellularly active organocatalysts - OGG1 organocatalytic switches assist the enzyme in reaction turnover and diversification of substrate and product scope. (ki.se)
Further, the formation of this functionality temporally removes OGG1 enzyme copies from the catalytic cycle. (ki.se)
Although OGG1 is no significant AP-lyase in cells, DNA glycosylases with pronounced β- or β,δ-AP lyase activity exist in humans and other species. (ki.se)

On the contrary, the stimulation in the appearance of the incision product seems to reflect the addition of the effects of XRCC1 on the two first enzymes of the pathway. (nih.gov)
Transient tyrosinemia is believed to result from delayed enzyme maturation in the tyrosine catabolic pathway. (medscape.com)

Testing and optimising the use of that enzyme to maximise assay performance. (biosearchtech.com)

These properties will then be compared to biochemical data, such as equilibrium binding constants to DNA glycosylases AAG and AlkD, and will be utilized as structural probes to gain insight regarding recognition and excision mechanisms employed by these enzymes. (hhs.gov)

Now, select the right enzymes and reagents with that performance in mind. (biosearchtech.com)
That's why the process of optimising enzymes and reagents is key. (biosearchtech.com)
From initial R&D development to larger-scale production, we manufacture critical raw materials in-house and provide your team with a reliable supply of enzymes and reagents, in the right concentrations and at the right time. (biosearchtech.com)

Expression and regulation of the mammalian SUMO-1 E1 enzyme. (nih.gov)
Mammalian cells have several families of DNA glycosylases to protect them from different types of DNA damage. (salford.ac.uk)

The mechanism requires a family of enzymes called glycosylases. (news-medical.net)
The molecular mechanism by which 2TX inhibits Fpg may be generalized to all prokaryote and eukaryote ZnF-containing Fpg/Nei-DNA glycosylases. (rcsb.org)
The question arises about the mechanism of substrate recognition for these enzymes and how closely it is linked to the base flipping step. (elsevierpure.com)

XRCC1 participation in these pathways is mediated by its interactions with several of the acting enzymes. (nih.gov)

However, this interpretation ignores enzymes with complex biochemistry, where substrate hydrolysis is achieved by consecutive steps of replacement and cleavage. (ki.se)

Orthologous to human UBA2 (ubiquitin like modifier activating enzyme 2). (nih.gov)
Cell experiments show that 2TX can operate in cellulo on the human Fpg/Nei DNA glycosylases. (rcsb.org)

Glycosylases (EC 3.2) are enzymes that hydrolyze glycosyl compounds. (wikipedia.org)
In turn, glycosylases are divided into two groups: glycosidases-enzymes that hydrolyze O- and S-glycosyl compounds (EC 3.2.1) -- and enzymes that hydrolyze N-glycosyl compounds (EC 3.2.2). (wikipedia.org)

Enables SUMO activating enzyme activity. (nih.gov)
The enzyme shows no activity on RNA or oligonucleotides. (qiagen.com)
[ 12 ] The activity of key mitochondrial enzymes of the tricarboxylic acid cycle, β-oxidation, and the electron transport system, conversely, were unchanged, [ 12 ] leaving many unanswered questions regarding the effects of CR on muscle-specific mitochondrial function in humans. (medscape.com)

Normally, the MUTYH gene gives instructions to make an enzyme called MYH glycosylase. (everydayhealth.com)

However, very little information regarding the effects of minor versus major groove lesions exist, in part due to their inherent instability with respect to spontaneous and enzyme-mediated depurination. (hhs.gov)

I am particularly interested in a family of DNA glycosylases called Nei-Like (NEIL) that were first discovered in 2002. (salford.ac.uk)

different DNA glycosylases can recognize similar types of damaged bases. (salford.ac.uk)

Therefore, my work is focused on determining the biological role of this DNA glycosylase using molecular and cell biology techniques in collaboration with colleagues in Manchester and Liverpool. (salford.ac.uk)

SMUG1 but not UNG DNA glycosylase contributes to the cellular response to recovery from 5-fluorouracil induced replication stress. (musc.edu)
We conclude that the rates of BER depend only in part on cellular levels of initiating enzymes. (utmb.edu)

DNA glycosylases are enzymes that remove damaged (chemically altered) bases from DNA in the first step of BER. (salford.ac.uk)
Enzymes that modify the bases often "flip out" the target into an extrahelical position before the chemistry step is carried out. (elsevierpure.com)

Homologues of bacteriophage T4 DNA glycosylase can find their application in pharmaceuticals and perfumery. (e3s-conferences.org)

Crystal structures of the enzyme bound to DNA in the presence of 2TX demonstrate that the inhibitor chemically reacts with cysteine thiolates of ZnF and induces the loss of zinc. (rcsb.org)

These limited differences in enzyme expression levels cannot readily explain the different rates at which the three lesions are repaired in vitro. (utmb.edu)

Selecting the right off-the-shelf or custom enzyme for your application. (biosearchtech.com)
Compost is a promising source of thermotolerant enzymes for their application in biotechnology. (e3s-conferences.org)

During the experiment, special sites, where single-stranded breaks can be introduced by adding specific enzymes in a test tube, were inserted into the DNA. (sciencedaily.com)

We look forward to being your trusted partner for current and future enzyme and molecular biology needs. (varizymes.com)

This enzyme helps correct errors that are sometimes made when DNA is copied before cell division. (everydayhealth.com)

Expand your enzyme portfolio with bulk & custom enzymes. (varizymes.com)

Anatomy8

Organisms21

Diseases1

Chemicals and Drugs105

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes64

Disciplines and Occupations1

Information Science3

Health Care1

Thymine DNA glycosy1

Uracil16

Superfamily1

Polymerase3

HOGG18

Oxoguanine4

Proteins3

Cytosine3

Metabolism1

Repair6

OGG13

Pathway2

Assay1

Structural1

Reagents3

Mammalian2

Mechanism3

Pathways1

Hydrolysis1

Human2

Compounds2

Activity3

Gene1

Spontaneous1

Family1

Recognize1

Biological1

Cellular2

Bases2

Pharmaceuticals1

Demonstrate1

Expression1

Application2

Single1

Future1

Errors1

Custom1