Based on catalytic domain similarities, β-Agarase II of Pseudoalteromonas atlantica has been designated part of the glycosyl hydrolase 86 (GH86) family. (worthington-biochem.com)
Glycoside hydrolase family 1 [Interproscan]. (ntu.edu.sg)
Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. (unl.edu)
This subfamily contains glycosyl hydrolase family 130 (GH130) proteins, as classified by the carbohydrate-active enzymes database (CAZY), are phosphorylases and hydrolases for beta-mannosides, and include beta-1,4-mannosylglucose phosphorylase (EC 2.4.1.281), beta-1,4-mannooligosaccharide phosphorylase (EC 2.4.1.319), among others that have yet to be characterized. (unl.edu)
This subfamily contains glycosyl hydrolase family 130 (GH130) proteins, as classified by the carbohydrate-active enzymes database (CAZY), most of which are as yet uncharacterized. (unl.edu)
Predicted glycosyl hydrolase, GH43/DUF377 family [Carbohydrate transport and metabolism]. (unl.edu)
Photosynthate within plants is transported from source to sink tissues as sucrose, which is hydrolyzed by plant glycosyl hydrolase family 32 enzymes (GH32) into its constituent monosaccharides to meet plant cellular demands. (elsevierpure.com)
Indeed, as one of the first glycoside hydrolase families classified by hydrophobic cluster analysis, GH9 was previously known as "Cellulase Family E" [ 1 , 2 ]. (cazypedia.org)
Catalytic1
We have characterized by NMR spectroscopy the three active site (His80, His85, and His205) and two non-active site (His107 and His114) histidines in the 34 kDa catalytic domain of Cellulomonas fimi xylanase Cex in its apo, noncovalently aza-sugar-inhibited, and trapped glycosyl-enzyme intermediate states. (ubc.ca)
Glycosyl transferases and carbohydrate esterases were enriched in loam, whereas glycoside hydrolases and carbohydrate-binding modules were enriched in clay loam. (bvsalud.org)
GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. (unl.edu)
The mutant enzyme is now able to work with thio-glycosides which are found in several plants and have shown both antimicrobial and anticancer activity. (paradisiresearch.com)
Protein1
In this study, we found 61 families of glycoside hydrolases (GH) (out of 182 CAZymes) from protein sequences deposited in the CAZy database-currently associated with more than 20,324 microbial genomes. (biomedcentral.com)
Glycosidic2
Glycoside hydrolase enzymes hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. (wikipedia.org)
Any member of the class of enzymes that catalyze the cleavage of the glycosidic linkage of glycosides and the addition of water to the resulting molecules. (bvsalud.org)
This paper presents Nourah's work on how strategic site directed mutagenesis has been successfully applied to shift substrate preference in glycosyl hydrolases. (paradisiresearch.com)
Substrate specificities == [[Glycoside hydrolases]] of GH84 include ''β''-''N''-acetylglucosaminidases but members have often also been annotated as ''β''-''N''-acetylhyaluronidases. (cazypedia.org)
Belong1
Other β-agarases belong to glycosyl hydrolase families 16 and 50, with all three groups containing a conserved glycoside hydrolase region responsible for catalysis (Fu and Kim 2010). (worthington-biochem.com)
Enzymatic1
S-Glycosides are very attractive substitutes for O-glycosides, as it is well-known that they are much less susceptible to enzymatic cleavage as well as chemical degradation [ 13 ]. (oncotarget.com)
The diverse ionization behaviors of the histidine residues are discussed in terms of their structural and functional roles in this model glycoside hydrolase. (ubc.ca)
Chemistry1
Moreover, they are promising candidates in synthetic carbohydrate chemistry as convenient and versatile glycosyl donors. (oncotarget.com)
Found1
About 70% of the adult world population is lactose-intolerant, due to low levels of intestinal lactase, also called lactase-phlorizin hydrolase (LPH), a β-D-galactosidase found in the apical surface of the intestinal microvilli. (researchgate.net)
Include1
GH130 enzymes are phosphorylases and hydrolases for beta-mannosides, and include beta-1,4-mannosylglucose phosphorylase (EC 2.4.1.281), beta-1,4-mannooligosaccharide phosphorylase (EC 2.4.1.319), beta-1,4-mannosyl-N-acetyl-glucosamine phosphorylase (EC 2.4.1.320), beta-1,2-mannobiose phosphorylase (EC 2.4.1. (unl.edu)
Enzymes9
Glycoside hydrolase enzymes hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. (wikipedia.org)
These enzymes catalyze the transfer a glycosyl group from cheap and readily available donor substrates (e.g. sucrose) to different acceptor molecules. (ugent.be)
This glycosyl hydrolase family 43 (GH43) includes enzymes that have been characterized with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55), endo-alpha-L-arabinanase as well as arabinoxylan arabinofuranohydrolase (AXH) activities. (unl.edu)
Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. (unl.edu)
This glycosyl hydrolase family 43 (GH43) includes enzymes that have been characterized with xylan-digesting beta-xylosidase (EC 3.2.1.37) and xylanase (endo-alpha-L-arabinanase) activities. (unl.edu)
Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F. This glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) includes family 43 (GH43) and 62 (GH62). (unl.edu)
Proteomic analysis of the T. aurantiacus culture supernatant revealed dominant glycoside hydrolases from families 5, 7, 10, and 61, proteins that are key enzymes in commercial cocktails. (biomedcentral.com)
Enterococcus faecalis is a bacterium with a vast range of genes encoding enzymes with putative glycoside hydrolyzing domains, but to date, very few have been characterized. (medscape.com)
Putative glycoside1
To test this hypothesis, we subcloned the putative glycoside hydrolase CcsZ and solved the X-ray crystal structure of both apo- and product-bound CcsZ. (biorxiv.org)
Much of the chemistry of glycosides is explained in the article on glycosidic bonds . (chemeurope.com)
Digestion of external cellulose requires the action of secreted cellulases, which belong to the glycoside hydrolase (GH) family of proteins and hydrolyse β-1,4 glycosidic bonds of the glucose polymer 4 . (nature.com)
Substrates2
Thermoascus aurantiacus and Thielavia terrestris were cultivated on various biomass substrates and culture supernatants assayed for glycoside hydrolase activities. (biomedcentral.com)
Supernatants from both cultures possessed comparable glycoside hydrolase activities when incubated with artificial biomass substrates. (biomedcentral.com)
Gene2
This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. (wikipedia.org)
Many authors require in addition that the sugar be bonded to a non-sugar for the molecule to qualify as a glycoside, thus excluding the polysaccharides . (chemeurope.com)
Depending on whether the glycosidic bond lies "above" or "below" the plane of the cyclic sugar molecule, glycosides are classified as α-glycosides or β-glycosides . (chemeurope.com)
Activity2
http://www.pdb.org/pdb/explore/explore.do?structureId=3KL0 3KL0] is a member of O-Glycosyl hydrolase family 30 as well, but does not carry out glucosylceramidase activity. (tu-muenchen.de)
We show that CcsZ is in fact capable of this activity and belongs to a broader family of glycoside hydrolases with unexpected taxonomic diversity. (biorxiv.org)
An example is apterin which is reported to dilate the coronary arteries as well as block calcium channels .those obtained from dried leaves of Psoralia corylifolia have Main glycosides psoralin and corylifolin. (chemeurope.com)
Includes2
The glycoside hydrolase 18 family of chitinases includes eight human family members. (wikipedia.org)
Glycosyl hydrolase family 43, includes mostly 1,4-beta-xylanases. (unl.edu)
Type1
We can classify glycosides by the glycone, by the type of glycosidic bond, and by the aglycone. (chemeurope.com)