Phosphorylase can only cleave α-1,4- glycosidic bond between adjacent glucose molecules in glycogen but branches exist as α-1,6 linkages. (wikidoc.org)
[7] This is a common method through which to cleave bonds, with an acid below the site of hydrolysis to lend a proton and a base above to deprotinate a water which can then act as a nucleophile . (wikidoc.org)
Carbohydrate1
are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. (wikipedia.org)
Hydrolysis1
During cellulosehydrolysis, endoglucanases first attack the amorphous area of the polysaccharide chain, breaking the β-1,4-glycosidic bonds releasing cellodextrin and creating new chain ends. (springeropen.com)
Chitin3
Given that chitin is not produced by mammals, it is intriguing that Mycobacterium tuberculosis, an exclusively human pathogen harbours Rv1987, a probable chitinase and secretes it. (bvsalud.org)
ChiD, a chitinase found in Bacillus circulans, hydrolyzes the 1,4-beta-linkages of N-acetylglucosamine in chitin and chitodextrins. (unl.edu)
PPL2 (Parkia platycephala lectin 2) is a class III chitinase from Parkia platycephala seeds that hydrolyzes beta(1-4) glycosidic bonds linking 2-acetoamido-2-deoxy-beta-D-glucopyranose units in chitin. (unl.edu)
Chitotriosidase1
BACKGROUND: Chitotriosidase (chitinase 1 or CHIT1) is secreted by activated macrophages. (bvsalud.org)
Hydrolyzes1
Lysozyme (EC 3.2.1.17) is an enzyme that hydrolyzes the β-1,4-glycosidic bond between N -acetylmuramic acid and N -acetylglucosamine (GlcNAc) in peptidoglycan, a major structural component of the bacterial cell wall ( Jollès and Jollès, 1984 ). (frontiersin.org)
Oomycetes were recently discovered as natural pathogens of Caenorhabditis elegans, and pathogen recognition alone was shown to be sufficient to activate a protective transcriptional program characterized by the expression of multiple chitinase-like (chil) genes. (bvsalud.org)
Chitinases1
These include sixteen endo-type chitinases, two N-acetylglucosaminidases, and one lipopolysaccharide monooxygenase that catalyzes the oxidative cleavage of glycosidic bonds. (bvsalud.org)
Cellulose3
It has been calculated that the natural cellulose crystalline area has 8 and the amorphous area at an average 5.3 hydrogen bonds per glucose unit (Kobayashi and Fukuoka 2013 ). (springeropen.com)
Cellulose is made up of glucose monomers that are linked by β-1-4 glycosidic bonds. (als-journal.com)
In the catalytic reaction, Glu35 is thought to act as a general acid catalyst to protonate the glycosidic oxygen, while Asp52 acts as a conjugate base and stabilizes the carbonium ion intermediate that adopts a half-chair conformation with C1 carbon displaying sp 2 hybridization. (frontiersin.org)
Single1
This is the release of four single chitinase deletion mutants in Salmonella enterica serovar Typhimurium LT2. (researchgate.net)
Domain1
Evidence for the formation of covalent bonds between macromolecules in the domain of the wall of Candida albicans mycelial cells. (microbiologyresearch.org)
Chitin5
Serratia marcescens Chitinase A (SmChiA) is a molecular motor efficiently hydrolyzing recalcitrant crystalline chitin by moving on the surface processively. (titech.ac.jp)
Chitinases (E.C.3.2.1.14) comprise a family of extracellular cell-wall hydrolases that hydrolyze the β-1,4-glycosidic bond of chitin to form the monomer N -acetyl-D-glucosamine. (ijbs.com)
Glycoside hydrolases that break down glycosidic bonds in CHITIN . (nih.gov)
PPL2 (Parkia platycephala lectin 2) is a class III chitinase from Parkia platycephala seeds that hydrolyzes beta(1-4) glycosidic bonds linking 2-acetoamido-2-deoxy-beta-D-glucopyranose units in chitin. (unl.edu)
The enzyme can also break glycosidic bonds in chitin , although not as effectively as true chitinases . (cloudfront.net)
This enzyme shows glycosidic hydrolyzing activity between the two GlcNAcs in the core of N-linked glycans and cleaves hybrid- and high-mannose-type glycans. (medscape.com)
The enzyme functions by hydrolyzing glycosidic bonds in peptidoglycans . (cloudfront.net)
Covalent intermediate of lysozyme enzyme, with covalent bond in black and experimental evidence as blue mesh. (cloudfront.net)
Substrate2
In the chemical step, glycosidic bond is first cleaved and intermediate structure of substrate is hydrolyzed. (titech.ac.jp)
We first identified a gene from B. thuringiensis YBT-9602 that encodes a chitinase (Chi9602) belonging to glycosyl hydrolase family 18 with conserved substrate-binding and substrate-catalytic motifs. (ijbs.com)
Covalent1
Evidence for the formation of covalent bonds between macromolecules in the domain of the wall of Candida albicans mycelial cells. (microbiologyresearch.org)
Molecular3
Recently, however, Serratia marcescenschitinase A (SmChiA) has been rediscovered as a molecular motor working in extracellular environments without using ATP. (titech.ac.jp)
Ni H, Zeng S, Qin X, Sun X, Zhang S, Zhao X, Yu Z, Li L. Molecular Docking and Site-directed Mutagenesis of a Bacillus thuringiensis Chitinase to Improve Chitinolytic, Synergistic Lepidopteran-larvicidal and Nematicidal Activities. (ijbs.com)
In this study, we constructed mutants of a Bacillus thuringiensis chitinase with enhanced activity based on homology modeling, molecular docking, and the site-directed mutagenesis of target residues to modify spatial positions, steric hindrances, or hydrophilicity/hydrophobicity. (ijbs.com)
Intermediate2
This proposed intermediate was also suggested to account for dispersing the charge developed at the anomeric carbon during bond breaking 10 . (nature.com)
[13] An ionic intermediate containing an oxo-carbenium is created as a result of the glycosidic bond breaking. (cloudfront.net)