• It is used in SDS-PAGE, agarose gel electrophoresis and histologic staining, e.g. staining of growth lines in bones. (wikipedia.org)
  • Cartilage extracted glycosaminoglycans (GAGs) concentration was assessed after agarose gel electrophoresis. (usp.br)
  • GAGs' molecular weight was evaluated after polyacrylamide gel electrophoresis. (usp.br)
  • Take this product than the curl, precision said, adding water to dissolve and diluted into each 1ml contains 2.5mg of solution, agarose gel electrophoresis test, the test and the ratio of the standard show spots by the migration distance is 0.9-1.1. (made-in-china.com)
  • Agarose gel electrophoresis of linear genomic DNA in the presence of ethidium bromide: band shifting and implications for forensic identity testing. (mcmaster.ca)
  • The mRNA expression of MMP-2 and MMP-9 was determined by reverse transcription-polymerase chain reaction, and the protein expression of MMP-2 and MMP-9 by gelatin zymography analysis and qualified by the gel electrophoresis image analysis in different HA concentrations. (molvis.org)
  • Using two-dimensional difference gel electrophoresis (2D-DIGE), proteomic analysis of the HeLa cells treated with a new compound is performed. (riken.jp)
  • The intact proteoglycan has a molecular size of approximately 150-165 kDa, containing heparan sulfate glycosaminoglycan chains of about 10-15 kDa. (umn.edu)
  • Mucopolysaccharidosis type II (MPS II) is a lysosomal storage disorder characterized by an accumulation of glycosaminoglycans (GAGs), including heparan sulfate, in the body. (bvsalud.org)
  • A quantidade de glicosaminoglicanos (GAGs) extraídos da cartilagem articular dos côndilos femorais foi medida após eletroforese em gel de agarose. (usp.br)
  • O tamanho molecular dos GAGs foi medido após eletroforese em gel de poliacrilamida. (usp.br)
  • We have studied the synthesis and secretion of sulfated glycosaminoglycans (GAGs) by the two cell types under control conditions and ß-D-xyloside-stimulated conditions, that stimulate the ability to synthesize and release GAGs. (embrapa.br)
  • Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels. (columbia.edu)
  • To isolate biotin labeled proteins, lysate was additional to immobilized NeutrAvidin TM Gel and incubated one hr at area temperature. (mirnainhibitor.com)
  • In this study, HSPGs extracted from embryonic rat spinal cord by detergent were purified by ionexchange chromatography, gel filtration, and affinity chromatography on an agarose column coupled with FN‐C/H II conjugated to ovalbumin (OA). (umn.edu)
  • Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (lookformedical.com)
  • The second model is based on a microstructural, molecular-level solution of the Poisson-Boltzmann (PB) equation within a unit cell containing a charged glycosaminoglycan (GAG) molecule and its surrounding atmosphere of mobile ions. (lievers.net)
  • Buschmann MD. Chondrocytes in Agarose Culture:​ Development of a Mechanically Functional Matrix, Biosynthetic Response to Compression, and Molecular Model of the Modulus [PhD thesis]. (lievers.net)
  • Hyaluronan (HA) is a multifunctional high molecular mass (HMM) glycosaminoglycan responsible for the maintenance of the extracellular matrix (ECM) of connective tissue. (biomedcentral.com)
  • CS-A,C and CS-A,C,K,L) and Carbopol 974P NF/agarose semi-interpenetrating polymer networks (i.e. (polimi.it)
  • Methods: This review aims to cover the different polymer-based gel types, the development and characterization methods, as well as applications thereof. (eurekaselect.com)
  • Results: In the last decades, polymer based pharmaceutical gels have shown attractive properties and therefore have raised the attention of pharmaceutical scientists. (eurekaselect.com)
  • Conclusion: The use of polymer-based gels as drug delivery systems would be beneficial for targeting numerous diseases. (eurekaselect.com)
  • The biosynthesis of HS happen within the Golgi apparatus and endoplasmic selleck inhibitor reticulum, exactly where the elongation of glycosaminoglycan chains is maintained by variety II glycosyltransferases encoded from the EXOSTOSINs genes, EXT1 and EXT2. (mirnainhibitor.com)
  • Mechanical stimulation led to a significant increase in chondrogenic gene expression, while histological analysis detected sulphated glycosaminoglycans and collagen II only in loaded specimens, confirming MC hydrogel suitability to support load induced MSCs chondrogenesis. (nature.com)
  • VOLPI N. Therapeutic applications of glycosaminoglycans[J]. Curr Med Chem, 2006, 13(15):1799-1810. (magtechjournal.com)
  • Alginate hydrogels are particularly attractive in wound healing, drug delivery, and tissue engineering applications, as these gels retain structural similarity to the extracellular matrices in tissues. (xantec.com)
  • ZHANG T M, LIN P X. Recent advances in glycosaminoglycan[J]. Food Drug(ʳƷ��ҩƷ), 2008, 10(9):1-5. (magtechjournal.com)
  • Siafaka, P.I. Promising polymeric drug carriers for local delivery: The case of in situ gels. (eurekaselect.com)
  • Stimuli-responsive gels based on stimuli-sensitive polymers are among the most studied gel class of last years. (eurekaselect.com)
  • Heparin Sodium (Heparin Sodium) is Heparin Sodium sulfate glycosaminoglycan anticoagulants.Heparin is from pigs or cows in the intestinal mucosa of extraction of amino dextran sulfate sodium salt, mucopolysaccharide material.In recent years, studies have shown that heparin and fall hematic fat action. (made-in-china.com)
  • Finally, the recent advancements and future perspectives focusing on radiolabeled gels will be addressed. (eurekaselect.com)
  • By ELISA, PCR, and 1,9-dimethyl methyleneblue technology, type I and type II collagen and glycosaminoglycan contents were quantitatively detected in cartilage cells 28 days after culture. (hugecn.com)
  • Gels are either chemically or physically cross-linked networks that can absorb fluids such as water (hydrogels), oil (organogels) and even air(aerogels). (eurekaselect.com)
  • After loading 1 mg CS/0.5 mL gel, we evaluated CS release up to 7 days by 1,9-dimethylmethylene blue (DMMB) assay and verified the neuroprotective role of CS-A,C (1 µM) in the supernatants. (polimi.it)
  • 5. A general method for the detection and mapping of submicrogram quantities of glycosaminoglycan oligosaccharides on polyacrylamide gels by sequential staining with azure A and ammoniacal silver. (nih.gov)
  • It consists, in varying proportions, of collagen, elastic fibers, proteoglycans (PGs), glycosaminoglycans (GAGs) and cellular elements, which are organized forming a network, which is partly responsible for the great morphological and functional diversity of the various tissues. (scielo.br)
  • Interaction of hyaluronan binding peptides with glycosaminoglycans in poly(ethylene glycol) hydrogels. (ucdenver.edu)
  • Hyaluronan (HA) is a major glycosaminoglycan component of the ECM and plays a significant role in regulating inflammation. (ashpublications.org)
  • 10. Synthesis and secretion of glycosaminoglycans in cultured retinal pigment epithelium. (nih.gov)
  • Schneiderman R, Keret D, Maroudas A. Effects of mechanical and osmotic pressure on the rate of glycosaminoglycan synthesis in the human adult femoral head cartilage:​ an in vitro study. (lievers.net)
  • Procedure -Chondrocytes from harvested specimens were cultured in 3-dimensional (3-D) agarose at 10 6 cells/mL. (avma.org)
  • Encapsulating chondrocytes in degrading PEG hydrogels with high modulus: engineering gel structural changes to facilitate cartilaginous tissue production. (ucdenver.edu)
  • Comparative study of the viscoelastic mechanical behavior of agarose and poly(ethylene glycol) hydrogels. (ucdenver.edu)
  • At both levels of intermittent pressure, the concentration of sulfated glycosaminoglycan in constructs from juvenile cells was found to be up to ten times greater than concentrations in control (nonpressurized) and adult cell-derived constructs. (lievers.net)
  • No. 4,582,865 (Biomatrix Inc.) describes the preparation of crosslinked gels of HA, alone or mixed with other hydrophilic polymers, using divinyl sulfone (DVS) as the crosslinking agent. (justia.com)
  • Samples of liquid media were collected on days 6, 9, 15, and 21 of culture for evaluation of glycosaminoglycan (GAG) and nitric oxide concentrations. (avma.org)