Loading...
  • overexpression
  • What we know about this process stems mostly from overexpression of tagged misfolded proteins, or from situations where viruses have hijacked the quality control machinery to their advantage. (jove.com)
  • Potential factors for MDR includes enhanced drug detoxification, decreased drug uptake, increased intracellular nucleophiles levels, enhanced repair of drug induced DNA damage, overexpression of drug transporter such as P-glycoprotein(P-gp), multidrug resistance-associated proteins (MRP1, MRP2), and breast cancer resistance protein (BCRP). (frontiersin.org)
  • enzymes
  • These include enzymes called ceramide-activated Ser-Thr phosphatases (CAPPs), such as protein phosphatase 1 and 2A (PP1 and PP2A), which were found to interact with ceramide in studies done in a controlled environment outside of a living organism (in vitro). (wikipedia.org)
  • On the other hand, studies in cells have shown that ceramide-inducing agents such as tumor necrosis factor-alpha α (TNFα) and palmitate induce the ceramide-dependent removal of a phosphate group (dephosphorylation) of the retinoblastoma gene product RB and the enzymes, protein kinases B (AKT protein family) and C α (PKB and PKCα). (wikipedia.org)
  • mRNA
  • act as control in mRNA production and protein synthesis. (getrevising.co.uk)
  • Regulated by MITF) HMGB1 High mobility group box binds DNA ILF2 Homo sapiens interleukin enhancer binding factor 2, 45kDa (ILF2), mRNA IER2 formerly ETR101 Immediate Early Protein? (wikipedia.org)
  • PRKAG1 NM_002733 Homo sapiens protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1), mRNA PRKAR1A NM_002734 Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells PRKRIP1 NM_024653 Binds double-stranded RNA. (wikipedia.org)
  • transmembrane
  • The two halves of the protein pack against each other in a clam-shell fashion, sealing via interactions at the ends of the transmembrane helices and extracellular loops. (wikipedia.org)
  • concentrations
  • Examples of the P-type ATPase include Na+/K+-ATPase that is regulated by Janus Kinase-2 as well as Ca2+ ATPase which exhibits sensitivity to ADP and ATP concentrations P-glycoprotein is an example of an ABC transport binding protein in the human body. (wikipedia.org)
  • halves
  • In the presented protocol the investigated candidate proteins are fused to complementary halves of fluorescent proteins and the respective constructs are introduced into plant cells via agrobacterium-mediated transformation. (jove.com)
  • gene
  • The protein encoded by this gene is a member of a family of membrane glycoproteins. (nih.gov)
  • When a person receives one changed form of the gene AGU from one of the parents, the individual is then classified as a carrier. (wikipedia.org)
  • DAXX Death Associated Protein 6 ARAF MAZ (gene) MYC also considered a transcription factor MCM3AP possibly a primase XRCC5 NM_021141 Ku80 XRCC6 NM_001469 Homo sapiens thyroid autoantigen: Single-stranded DNA-dependent ATP-dependent helicase. (wikipedia.org)
  • recombinant
  • Transient expression of recombinant CDV F and H surface glycoproteins in Vero cells and primary hippocampal neurons further confirmed a correlation between their accumulation in the ER, CRT upregulation, ER stress and disruption of ER Ca(2+) homeostasis. (jove.com)
  • cytosolic
  • In the ER, the catalytic A chain dissociates from the rest of the toxin and moves through a protein-conducting channel to reach its cytosolic target 5 . (jove.com)
  • simultaneous
  • Proteomics analysis allows the simultaneous analysis of thousands of proteins in a sample. (beds.ac.uk)
  • The latest TED vectors contain a core element of CES2 fused to a red fluorescent protein, thus enabling simultaneous two-color imaging. (jove.com)
  • interactions
  • BiFC is ideally suited to study protein-protein interactions in their natural surroundings within the living cell. (jove.com)
  • Nevertheless, BiFC is an excellent complementary approach to other commonly applied methods investigating protein-protein interactions, such as coimmunoprecipitation, in vitro pull-down assays or yeast-two-hybrid experiments. (jove.com)
  • We also employed defined perturbations of cellular pathways paired with systems biology approaches to uncover protein-protein and protein-small molecule interactions. (nih.gov)
  • examples
  • We know of only very few endogenous substrates of ER quality control, most of which are degraded as part of a signaling pathway, such as Insig-1, but such examples do not necessarily represent terminally misfolded proteins. (jove.com)
  • defective
  • Proteins deemed terminally misfolded and hence functionally defective may be dislocated to the cytosol, where the proteasome degrades them. (jove.com)
  • phosphate
  • Whether the protein is activated or inhibited with the addition of the phosphate group depends on the specific protein. (wikipedia.org)
  • The first structures were of the glycerol 3-phosphate/phosphate exchanger GlpT and the lactose-proton symporter LacY, which served to elucidate the overall structure of the protein family and provided initial models for understanding the MFS transport mechanism. (wikipedia.org)