Antibodies to p62 complex are involved in one or more autoimmune diseases. (wikipedia.org)
Cytoplasmic4
p62 is synthesized as a soluble cytoplasmic precursor of 61 kDa followed by modification that involve addition of N-acetylglucosamine residues, followed by association with other complex proteins. (wikipedia.org)
The p62 complex is localized to both the nucleoplasmic and cytoplasmic sides of the pore complex and the relative diameter of p62 complex relative to the nuclear pore complex suggests it interacts in pore gating. (wikipedia.org)
Systemic lupus erythematosus (SLE) is a systemic autoimmune disease which is classically characterised by a variety of autoantibodies to deoxyribonucleic acid (DNA), ribonucleic acid (RNA), other nuclear and cytoplasmic antigens. (openrheumatologyjournal.com)
NLS-importin-α complexes connect to nuclear pore complexes through importin-β and so are translocated in to the nucleus within an energy-dependent procedure (19 24 BIG1 was defined in HepG2 cells colocalized partly with nucleoporin p62 on the nuclear envelope probably in transit between nuclear and cytoplasmic compartments (13). (molecularcircuit.com)
Antibodies3
In this paper we evaluate anti-nucleoporin p62 antibodies by western blot analysis in 25 systemic lupus erythematosus patients. (openrheumatologyjournal.com)
Our data indicate that p62 antibodies could be a useful additional marker in SLE. (openrheumatologyjournal.com)
Isoform1
Multiple transcript variants of this gene encode a single protein isoform. (wikipedia.org)
Membrane5
Due to the substantial progress in elucidation of the structure of the nuclear membrane and pore complex, recently novel autoantigens localised within the nucleus and the nuclear envelope have been investigated in more detail. (openrheumatologyjournal.com)
2 ] differentiate the nuclear envelope (NE) into five regions, the outer nuclear membrane (ONM), an inter membrane space, the inner nuclear membrane (INM), the underlying nuclear lamina and the nuclear pore complex (NPC). (openrheumatologyjournal.com)
Recent work published in The EMBO Journal and EMBO Reports reveals a novel role for the protein TECPR1 as a sensor for stressed membranes and regulator of lysosomal membrane repair. (babraham.ac.uk)
However, the Atg8 family of proteins are not found solely attached to double-membrane autophagosomes. (babraham.ac.uk)
Membrane proteins must be threaded co-translocationally into the lipid bilayer to become membrane-integrated, often with complex topologies and typically form hetero- or homo- oligomers. (stanford.edu)
Intracellular4
Glycans, either alone or complexed with glycan-binding proteins, can deliver intracellular signals or control extracellular processes that promote initiation, execution and resolution of cell death programs. (nature.com)
Mechanisms by which E. chaffeensis establishes intracellular infection, and avoids host defenses are not well understood, but involve functionally relevant host-pathogen interactions associated with tandem and ankyrin repeat effector proteins. (frontiersin.org)
Despite its small genome and limited number of effector proteins, Ehrlichia efficiently establishes an intracellular infection and avoids immune defenses in vertebrate and invertebrate hosts through complex molecular and cellular reprogramming strategies. (frontiersin.org)
A-inhibited guanine nucleotide-exchange protein BIG1 and BIG2 are activators of ADP-ribosylation SNS-314 aspect GTPases which are needed for regulating vesicular visitors among intracellular organelles. (molecularcircuit.com)
Cytoplasm3
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. (wikipedia.org)
P62 also interacts with a nuclear transport factor (NTF2) protein that is involved in trafficking proteins between cytoplasm and nucleus. (wikipedia.org)
Proteins bigger than 45 kDa need a nuclear localization series (NLS) for entrance in to the nucleus (15). (molecularcircuit.com)
The NLS within a proteins destined for nuclear localization includes a unipartite or even a bipartite simple amino acidity cluster such as for example KKKRK in SV40 huge tumor antigen (25) or RKR-Xn-RKRKR in T cell proteins tyrosine phosphatase (26) that is acknowledged by an importin-α/β heterodimer. (molecularcircuit.com)
Proteins phosphorylation near the NLS is normally reported to try out a major function in modulating NLS-dependent nuclear import and will facilitate NLS identification with the NLS-binding importin-α subunit (23 27 28 Proteins kinases including PKA regulate the subcellular localization of several protein. (molecularcircuit.com)
Mutation from the NLS in BIG1 led to the lack of its nuclear localization with or without cAMP treatment. (molecularcircuit.com)
P62 appears to interact with mRNA during transport out of the nucleus. (wikipedia.org)
Structural2
At that time, glycobiology, which is the study of carbohydrates and their recognition by motif-specific carbohydrate-binding proteins or lectins, lagged far behind the studies that defined the structural and cellular biology of cell death. (nature.com)
IN is a 32 kDa protein with three distinct structural domains, the N-terminal zinc-binding domain, the central catalytic core domain and the C-terminal domain. (biomedcentral.com)
Extracellular1
This study presents an in-silico model replicating a microfluidic assay where endothelial cells sprout into a biomimetic extracellular matrix, specifically, a large-pore, low-concentration fibrin-based porous hydrogel, influenced by chemotactic factors. (ibecbarcelona.eu)
Subunit1
Nuclear importation of proteins is really a two-step procedure relating to the dimeric importin-α/β where the α-subunit straight binds the NLS theme and acts as an adaptor for importin-β. (molecularcircuit.com)
Amino1
P62 is a serine/threonine rich protein of ~520 amino acids, with tetrapeptide repeats on the amino terminus and a series of alpha-helical regions with hydrophobic heptad repeats forming beta-propeller domain. (wikipedia.org)
Cellular1
This is driven by cellular proteins which tether the lentiviral preintegration complexes to specific sites on the host chromosomes. (biomedcentral.com)
Antigen2
BIG1 is really a proteins of just one 1 849 aa using a forecasted NLS series 711KKPKR715 which is one of the same course of monopartite NLS modules within SV40 T antigen. (molecularcircuit.com)
anti nf kb p65 antibody 783 anti stat6 antibody 784 anti cd 19 antibody 785 anti cd 38 antibody 786 anti pten ab 787 anti ck9 antibody 788 anti gh antibody 789 anti lh beta antibody 790 anti tsh alpha antibody 791 anti fsh alpha antibody 792 anti hpv antibody 793 anti ebv nuclear antigen ab 794 anti ebv latent memb. (tendersindelhi.com)
Compartments1
This highly complex 'protein biogenesis' process is assisted by a diverse network of folding catalysts and protein-modifying enzymes and is scrutinized by molecular chaperones and other 'quality control' factors which ensure that only correctly folded and assembled proteins exit the ER and proceed to distal compartments of the secretory pathway. (stanford.edu)
Interaction1
and ?and44translated epitope-tagged proteins Li (18) confirmed the interaction of RIα with SNS-314 BIG2. (molecularcircuit.com)
Nucleoporins2
Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. (wikipedia.org)
The protein encoded by this gene is a member of the FG repeat containing nucleoporins and is localized to the nuclear pore central plug. (wikipedia.org)
Efficiently1
Instead, damaged or incorrectly synthesized proteins must be rapidly and efficiently destroyed lest they form toxic aggregates. (stanford.edu)
Regulates1
The V-ATPase complex regulates non-canonical Atg8-family protein lipidation through ATG16L1 recruitment. (babraham.ac.uk)
Pathway4
These ATG proteins also perform an important parallel role in 'noncanonical' autophagy, a lysosome-associated signaling pathway with key functions in immunity, inflammation, cancer, and neurodegeneration. (babraham.ac.uk)
The vast majority of proteins entering the secretory pathway are synthesized on ribosomes docked at ER translocons and are co-transationally translocated into the ER lumen. (stanford.edu)
Our goal is to elucidate the functional networks that coordinate protein synthesis and quality control in the early secretory pathway. (stanford.edu)
We established that the tailored contractile response constitutes a nuclear ruler based signaling pathway involved in migratory cell behaviors. (regenerativemedicine.net)
Transporter1
Karyopherin beta2 (transportin), a riboprotein transporter also interacts with p62. (wikipedia.org)
Family3
Conjugation of the Atg8 (autophagy related 8) family of ubiquitin-like proteins to phospholipids of the phagophore is a hallmark of macroautophagy/autophagy. (babraham.ac.uk)
In non-canonical Atg8-family protein lipidation they become conjugated to single membranes. (babraham.ac.uk)
We have shown that this process is triggered by recruitment of ATG16L1 by the vacuolar-type H-translocating ATPase (V-ATPase) proton pump, suggesting a role for pH sensing in recruitment of Atg8-family proteins to single membranes. (babraham.ac.uk)
Cells2
The surface of the living cells is decorated by a complex layer of glycosylated molecules that store relevant biological information. (nature.com)
Cells rely on the nuclear ruler to modulate the motive force that enables their passage through restrictive pores in complex three-dimensional environments, a process relevant to cancer cell invasion, immune responses, and embryonic development. (regenerativemedicine.net)
Rich1
O-GlcNAcylation appears to be involved in the assembly and disassembly of p62 into higher order complexes, and a serine/threonine rich linker region between Ser270 to Thr294 appear to be regulatory. (wikipedia.org)
Cell death2
Herein, we review the role of glycans and glycan-binding proteins as essential components of the cell death machinery during physiologic and pathologic settings. (nature.com)
We decellularized the metastatic lung sections, measured the micromechanics of the tumor ECM, and stained the sections for ECM proteins, proliferation, and cell death markers. (ibecbarcelona.eu)
Process1
Autophagy is a fundamental catabolic process coordinated by a network of autophagy-related (ATG) proteins. (babraham.ac.uk)
Essential2
Mutagenesis of the site in BIG1 demonstrated which the PKA-catalyzed phosphorylation of Ser-883 was essential SNS-314 for nuclear deposition of RHOF BIG1 in response to 8-Br-cAMP. (molecularcircuit.com)
PKA-catalyzed phosphorylation of Ser-883 was essential for nuclear translocation however not sufficient as the existence of an operating NLS was needed. (molecularcircuit.com)
Influence1
P62 glycosylation is increased in diabetes and may influence its association with other diseases. (wikipedia.org)