Glycoprotein binding sites antibody. Medical search. Frequent questions

Human monoclonal antibodies are characterized to study the humoral immune response in these disorders. (umassmed.edu)
Not only do we generate new human monoclonal antibodies for our studies, but also for a large number of laboratories throughout the world for research on infectious diseases, cancer and autoimmune disease. (umassmed.edu)
We collaborate with Drs. Greiner, Brehm and Luban here at UMMS and Dr. Leonard Schultz at Jackson Laboratories on humanized mouse models for the generation of human monoclonal antibodies and as models for passive immunotherapy for bacterial or viral infections. (umassmed.edu)
The laboratory has also been involved in pharmacokinetic and pharmacodynamic studies of IgG and IgA human monoclonal antibodies, in several bacterial and viral infections. (umassmed.edu)
Enterotoxigenic Escherichia coli,, Bordetella pertussis, Klebsiella pneumonia), she is also inventor of two human monoclonal antibodies against Staphylococcus aureus and Pseudomonas aeruginosa. (umassmed.edu)
Formulation Studies to Develop Low-Cost, Orally-Delivered Secretory IgA Monoclonal Antibodies for Passive Immunization Against Enterotoxigenic Escherichia coli. (umassmed.edu)
Novel anti-CD4 monoclonal antibodies separate human immunodeficiency virus infection and fusion of CD4+ cells from virus binding. (jenner.ac.uk)
Previous studies have localized the high affinity binding site for gp120 to the first domain of CD4, and monoclonal antibodies (mAbs) reactive with this region compete with gp120 binding and thereby block virus infectivity and syncytium formation. (jenner.ac.uk)
Monoclonal antibodies to the FLAG epitope served to monitor cell surface expression for functional analysis of the BFIV21 class I glycoprotein. (usda.gov)
To study antibody modulation of HIV-1 Env-Galcer interactions, we used Galcer-containing liposomes to assess whether natural- and vaccine-induced monoclonal antibodies can block HIV-1 Env binding to Galcer. (duke.edu)
Several HIV-1 ALVAC/AIDSVAX vaccinee-derived monoclonal antibodies (MAbs) specific for the gp120 first constant (C1) region blocked Galcer binding of a transmitted/founder HIV-1 Env gp140. (duke.edu)
C1-specific IgG monoclonal antibodies that blocked Env binding to Galcer induced upregulation of the gp120 CD4-inducible (CD4i) epitope bound by MAb 17B, demonstrating that a conformational change in gp120 may be required for Galcer blocking. (duke.edu)
The surface GP is structurally different from the small nonstructural glycoprotein (GP) and is not recognized by virus-neutralizing monoclonal antibodies. (lapizgrafico.com)
The first drug approved in October 2020, Inmazeb™ , is a combination of three monoclonal antibodies. (cdc.gov)
Monoclonal antibodies (often abbreviated as mAbs) are proteins produced in a lab or other manufacturing facility that act like natural antibodies to stop a germ such as a virus from replicating after it has infected a person. (cdc.gov)
Human polyclonal and monoclonal antibodies against pathogens and toxins are potentially useful in the treatment of various diseases. (lu.se)
A number of human monoclonal antibodies with protective capacity in vitro have been established by conventional hybridoma technology. (lu.se)

Integrin alpha 2 beta 1 is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). (novusbio.com)
Human IL-17 RC is a type I transmembrane glycoprotein that is expressed on a variety of nonhematopoietic cell types. (rndsystems.com)
CD229, also known as Ly9 and SLAMF3, is a 120 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). (rndsystems.com)

Different retroviruses vary widely in N-linked glycosylation sites: HIV-1 can have as many as 30 sites glycosylated, 25 of which reside in gp120. (wikipedia.org)
Exposed on the surface of the viral envelope, the glycoprotein gp120 binds to the CD4 receptor on any target cell that has such a receptor, particularly the helper T-cell. (wikipedia.org)
Included are CD4 ANTIGENS, found on T4 lymphocytes, and monocytes/macrophages, which bind to the HIV ENVELOPE PROTEIN GP120. (harvard.edu)
Scholars@Duke publication: Structures of the CCR5 N terminus and of a tyrosine-sulfated antibody with HIV-1 gp120 and CD4. (duke.edu)
The CCR5 co-receptor binds to the HIV-1 gp120 envelope glycoprotein and facilitates HIV-1 entry into cells. (duke.edu)
Its N terminus is tyrosine-sulfated, as are many antibodies that react with the co-receptor binding site on gp120. (duke.edu)
We applied nuclear magnetic resonance and crystallographic techniques to analyze the structure of the CCR5 N terminus and that of the tyrosine-sulfated antibody 412d in complex with gp120 and CD4. (duke.edu)
These results now provide a framework for understanding HIV-1 interactions with the CCR5 N terminus during viral entry and define a conserved site on gp120, whose recognition of sulfotyrosine engenders posttranslational mimicry by the immune system. (duke.edu)
The CD4 binding site (CD4bs) of envelope glycoprotein (Env) gp120 is a functionally conserved, important target of anti-human immunodeficiency virus type 1 (HIV-1) neutralizing antibodies. (biomedcentral.com)
Our results showed that an amino acid substitution at position 185 in the V2 region of gp120 played a crucial role in regulating the b12 susceptibility of AE-Env-recombinant viruses by cooperating with 2 previously reported potential N-linked glycosylation (PNLG) sites at positions 186 (N186) and 197 (N197) in the V2 and C2 regions of Env gp120. (biomedcentral.com)
We propose that the V2 and C2 regions of AE-Env gp120 contain the major determinants of viral resistance to CD4bs antibodies. (biomedcentral.com)
The envelope glycoproteins (Env), gp120 and gp41, of human immunodeficiency virus type 1 (HIV-1) play a central role in viral transmission and mediate attachment and incorporation of the virus into target cells through specific interactions with the CD4 receptor and chemokine co-receptors [ 1 ]. (biomedcentral.com)
Human immunodeficiency virus (HIV) binds to cells via an interaction between CD4 and the virus envelope glycoprotein, gp120. (jenner.ac.uk)
Despite a detailed understanding of the binding of gp120 to CD4, little is known of subsequent events leading to membrane fusion and virus entry. (jenner.ac.uk)
Binding of recombinant gp120 or virus to CD4 is not inhibited by these antibodies, whereas infection and syncytium formation by a number of HIV isolates are blocked. (jenner.ac.uk)
Early efforts to develop HIV vaccines attempted to use the virus glycoprotein, gp120, to induce neutralising antibody, but did not take into account the trimeric structure of the native glycoprotein or the complex nature of the CD4 and chemokine receptor binding sites. (ox.ac.uk)
Scholars@Duke publication: Vaccine-induced HIV-1 envelope gp120 constant region 1-specific antibodies expose a CD4-inducible epitope and block the interaction of HIV-1 gp140 with galactosylceramide. (duke.edu)
However, the MAb 17B itself did not block Env-Galcer binding, suggesting that the C1 antibody-induced gp120 conformational changes resulted in alteration in a Galcer binding site distant from the CD4i 17B MAb binding site. (duke.edu)
Cross-links were also observed within gp120 at sites associated with the N241/N289 glycan hole that locally modified trimer antigenicity. (ox.ac.uk)

Co-receptor Binding Site Antibodies Enable CD4-Mimetics to Expose Conserved Anti-cluster A ADCC Epitopes on HIV-1 Envelope Glycoproteins. (harvard.edu)
Presence of both antibodies delivers a blocking action of the SARS-COV RBD-ACE2 interaction, by binding two distinct and functional epitopes (16796401). (abcam.com)
Functional analysis employing site directed mutagenesis identified BF amino acid residues forming serologic epitopes as well as residues important in antigen presentation to ALV induced cytotoxic T lymphocytes. (usda.gov)
The binding also involves the GPIb-binding site of vWF located on the A1 domain because it was inhibited by MoAb to vWF whose epitopes are within this domain and that block binding of vWF to platelets induced by ristocetin or botrocetin. (ashpublications.org)
We further demonstrate the existence in serum of protective neutralizing antibodies targeting co-dominant fusion (F) glycoprotein epitopes. (elsevierpure.com)
We propose that additional investigation into the role of ADCP in protective viral responses, the specific virus epitopes targeted by ADCP antibodies, and the types of phagocytes and Fc receptors involved in ADCP at sites of virus infection will provide insight into strategies to successfully leverage this important immune response for improved antiviral immunity through rational vaccine design. (frontiersin.org)
Antibodies recognize specific configurations (epitopes, or antigenic determinants) on the surfaces of antigens (eg, proteins, polysaccharides, nucleic acids). (msdmanuals.com)
The same antibody molecule can cross-react with related antigens if their epitopes are similar enough to those of the original antigen. (msdmanuals.com)

The SPD-type variant exhibits IgA autoantibodies targeting the transmembrane glycoprotein desmocollin 1 present within keratinocyte desmosomes. (medscape.com)

Because of the high prevalence of these inactive forms, the immune system often produces antibodies which target inactive gp160, rather than active forms of the envelope protein. (wikipedia.org)
Global site-specific N-glycosylation analysis of HIV envelope glycoprotein. (upenn.edu)
Effects of partially dismantling the CD4 binding site glycan fence of HIV-1 Envelope glycoprotein trimers on neutralizing antibody induction. (upenn.edu)
Characterization of Human Immunodeficiency Virus (HIV-1) Envelope Glycoprotein Variants Selected for Resistance to a CD4-Mimetic Compound. (harvard.edu)
Modulation of primary human immunodeficiency virus type 1 envelope glycoprotein-mediated entry by human antibodies. (microbiologyresearch.org)
Recently, we and others have shown that the interaction between envelope specific antibodies and primary human immunodeficiency virus type 1 (HIV-1) isolates may result in either inhibition or enhancement of virus entry. (microbiologyresearch.org)
To study the mechanism underlying this phenomenon, a series of HIV-1 envelope glycoproteins from closely related primary virus isolates of different syncytium inducing phenotypes, together with chimeras of these proteins, were tested in an envelope transcomplementation assay for their sensitivity to either antibody mediated inhibition or enhancement of HIV-1 entry. (microbiologyresearch.org)
The susceptibility of the envelope glycoprotein chimeric viruses to neutralization or enhancement of infectivity proved to be primarily determined by the configuration of the V3 loop, and the affinity of the antibodies to monomeric HIV-1 gp160 molecules, proved to be of quantitative importance only. (microbiologyresearch.org)
Both the V2 and V3 regions of the human immunodeficiency virus type 1 surface glycoprotein functionally interact with other envelope regions in syncytium formation. (microbiologyresearch.org)
The HIV-1 envelope glycoprotein (Env) is the sole viral target of bnAbs, but is also targeted by binding, non-neutralizing antibodies. (plos.org)
IMPORTANCE: Galactosyl ceramide, a glycosphingolipid, has been postulated to be a receptor for the HIV-1 envelope glycoprotein (Env) interaction with mucosal epithelial cells. (duke.edu)
The CBAs act at an early step of DENV infection as they bind to the viral envelope of DENV and subsequently prevent virus attachment. (unboundmedicine.com)
Structural and immunologic correlates of chemically stabilized HIV-1 envelope glycoproteins. (ox.ac.uk)
Inducing broad spectrum neutralizing antibodies against challenging pathogens such as HIV-1 is a major vaccine design goal, but may be hindered by conformational instability within viral envelope glycoproteins (Env). (ox.ac.uk)
Neutralization-guided design of HIV-1 envelope trimers with high affinity for the unmutated common ancester of CH235 lineage CD4bs broadly neutralizing antibodies. (ox.ac.uk)
The CD4 binding site (CD4bs) of the HIV-1 envelope glycoprotein is susceptible to multiple lineages of broadly neutralizing antibodies (bnAbs) that are attractive to elicit with vaccines. (ox.ac.uk)
Broadly neutralizing antibodies (bNAbs) against the membrane-proximal external region (MPER) of the gp41 component of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) are characterized by long, hydrophobic, heavy chain complementarity-determining region 3s (HCDR3s) that interact with the MPER and some viral membrane lipids to achieve increased local concentrations. (stanford.edu)
The same regions are also important for viral sensitization to neutralizing antibodies, directly linking the antiviral activity of SERINC5 with remodeling of the HIV-1 envelope glycoprotein. (crick.ac.uk)

To further develop Dr. Cavacini's background in mucosal immunology, included in this work is structural modeling of antibody/antigen interactions to improve the design of immunotherapeutic antibodies and development of a platform for production of dimeric and secretory IgA. (umassmed.edu)
BF residues 78 and 81 (HLA 79 and 82) form an antibody epitope with a slight effect on ALV antigen presentation, consistent with their predicted orientation based on the HLA-A2 crystal structure. (usda.gov)
In contrast, a site directed substitution in residue 34 dramatically alters ALV antigen presentation by the BFIV21 class I glycoprotein. (usda.gov)
Antigen stimulation of lymphocytes induces CD229 clustering to sites of T cell‑B cell contact (7). (rndsystems.com)
The apical portion of the variable regions serves as antigen-binding site. (mariamman.net)
The immunoglobulin may also be described in this way: it is composed of two Fab (fragment, antigen-binding) regions and one fc (fragment, crystallizable) region. (mariamman.net)
The Fabs include the antigen-combining sites whereas the fc region consists of the remaining constant sequence domains of the heavy chain s, cell-binding site, and complement-binding sites. (mariamman.net)
The antibody is directed against the leukocyte common antigen (L-CA) molecular weight 180-240kDa, present on all leukocytes, but not erythroid cells or cells from other tissues. (cancertools.org)
antigen has the same distribution as OX-1 and recognises the same number of sites. (cancertools.org)
Antiviral activities of antibodies may either be dependent only on interactions between the antibody and cognate antigen, as in binding and neutralization of an infectious virion, or instead may require interactions between antibody-antigen immune complexes and immunoproteins or Fc receptor expressing immune effector cells. (frontiersin.org)
The Fc receptor-dependent function of antibody-dependent cellular phagocytosis (ADCP) provides mechanisms for clearance of virus and virus-infected cells, as well as for stimulation of downstream adaptive immune responses by facilitating antigen presentation, or by stimulating the secretion of inflammatory mediators. (frontiersin.org)
Fc receptor-dependent antibody functions are also involved in activation of downstream adaptive immune responses by facilitating antigen presentation or by stimulating the secretion of inflammatory mediators ( 12 , 13 ). (frontiersin.org)
However, with the development of phage-display technology, the possibility of specifically tailoring antigen-binding properties has improved substantially. (lu.se)

These Fc receptor-dependent antibody functions provide a direct link between the innate and adaptive immune systems by combining the potent antiviral activity of innate effector cells with the diversity and specificity of the adaptive humoral response. (frontiersin.org)
The fine specificity, as determined by the requirement of particular amino acid residues in the epitope, is shifted in these new antibody fragments. (lu.se)

Tailored Immunogens Direct Affinity Maturation toward HIV Neutralizing Antibodies. (upenn.edu)
CR3022 has been shown to bind with a high affinity to SARS-CoV2 (32416259, 32413276). (abcam.com)
One human monoclonal antibody directed against gp41 (IAM 2F5) inhibited entry of all the viruses studied, irrespective of their phenotype, and directly proportional to its affinity to monomeric HIV-1 gp160. (microbiologyresearch.org)
Immunostaining with an affinity-purified mouse antibody reveals that Eph is highly targeted to axons and growth cones of developing neurons within the VNC. (sdbonline.org)
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
SHBG has a high binding affinity to dihydrotestosterone (DHT), medium affinity to testosterone and estradiol, and only a low affinity to estrone, DHEA, androstendione, and estriol. (cdc.gov)
Albumin, which exists in far higher concentrations than SHBG, also binds sexual steroids - although with a clearly lower binding affinity (e.g. about 100 times lower for testosterone). (cdc.gov)
CH235 UCA bound with nanomolar affinity to corresponding soluble native-like Env trimers as candidate immunogens. (ox.ac.uk)
Here, we show that increasing the local concentration of MPER-directed bNAbs at the cell surface via binding to the high-affinity Fc receptor FcγRI potentiates their ability to prevent viral entry in a manner analogous to the previously reported observation wherein the lipid-binding activity of MPER bNAbs increases their concentration at the viral surface membrane. (stanford.edu)
We show here that the reactivity of a high affinity, virus-neutralizing human antibody against the AD-2 epitope of cytomegalovirus gB can be modified by introducing other Vkappa sequences together with the original VH sequence. (lu.se)
It was also evident that the VH/Vkappa pairing was not promiscuous, since antibody fragments selected by phage display retained light chain sequences very similar to the original hybridoma-derived light chain, proving that a high affinity interaction was very dependent on a co-operativity between both variable domains. (lu.se)
These findings show that phage display technology might modify the binding properties of pre-existing, high affinity antibodies. (lu.se)

The group singled out the B cells - those that produce the antibodies that make up our "immune memory" - and conducted deep sequencing, tracing the lineages of those cells and isolating individual antibodies that bound to viral proteins. (weizmann.ac.il)
Most of the circulating testosterone is bound to carrier proteins (SHBG = sex hormone-binding globulin). (cdc.gov)
The expertise at MassBiologics, particularly in the areas of Discovery and Process Development, has allowed rapid production of SARS CoV-2 proteins (including several spike proteins and N protein) and human antibodies and nanobodies , contributing to the evaluation and development of therapeutics and diagnostics. (umassmed.edu)
HIV-1 Env gp140 proteins bound to Galcer liposomes with Kds (dissociation constants) in the nanomolar range. (duke.edu)
Here, we have mimicked this interaction by using an artificial membrane containing synthetic Galcer and recombinant HIV-1 Env proteins to identify antibodies that would block the HIV-1 Env-Galcer interaction. (duke.edu)
2-6 In vitro, the binding of vWF to GPIb can be promoted by the interaction of vWF with nonphysiological inducers like the antibiotic ristocetin or the snake venom proteins botrocetin or bitiscetin. (ashpublications.org)
Platelet activation allows binding of these proteins, which bridges adjacent platelets. (medscape.com)
Unlike typical immunoglobulin G (IgG)-mediated pemphigus, IgA pemphigus is characterized by tissue-bound and circulating IgA autoantibodies that target the desmosomal proteins of the epidermis. (medscape.com)
Some individuals with these autoimmune disorders produce immune proteins called antibodies that attack and destroy the GPVI protein. (medlineplus.gov)
Serum amyloid A and alpha-1 acid glycoprotein are transport proteins, and fibrinogen is a coagulation factor. (msdmanuals.com)

The primary neutralizing epitope (PNE), which is located on glycoprotein 5 and composed of the residues S 37 H(F/L)QLIYN with F/L 39 as the binding site for the neutralizing antibody ( 6 , 7 ), also displayed similar changes at the 39 position among the 4 subgroups. (cdc.gov)
The binding epitope is only accessible in the "open" conformation of the spike protein (Joyce et al. (abcam.com)
The binding epitope of clone CR3022 does not overlap with the ACE2 binding site of SARS-COV2 (32065055). (abcam.com)
A unique, chimeric class I glycoprotein was constructed by incorporating an epitope tag (FLAG) at the N terminus. (usda.gov)

The high anti-platelet efficacy of antibodies or antagonists for GPIIb/IIIa is explained by their interference with this final common event. (justia.com)
Thus, our data support the hypothesis that an interaction between the A1 and A3 domains exists that may play a role in the function of vWF by regulating the ability of the A1 domain to bind to platelet GPIb. (ashpublications.org)
Platelet glycoprotein VI-related clinical defects. (medlineplus.gov)

Recombinant Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab273073) is a Human IgG1 Recombinant version of CR3022 for research use only. (abcam.com)
Efficient neutralization of primary isolates of HIV-1 by a recombinant human monoclonal antibody. (microbiologyresearch.org)
Recombinant Fab fragments recognizing different conformations of this antigenic site have similar neutralization activities in spite of converse kinetic binding parameters. (ox.ac.uk)
We report that the variable domains of recombinant HIV-1 Env immunogens activate a large number of B cell clones that give rise to many non-neutralizing antibodies, and that removing the variable domains from the immunogen reduces the number of activated B cell lineages and leads to the development of autologous neutralizing antibodies, a step towards bNAb-production. (plos.org)
The 4.2 Å structure of HIV-1 BG505 SOSIP soluble recombinant Env in complex with a CD4 binding site-specific broadly neutralizing antibody (bNAb) Fab fragment reveals how cross-linking affects key properties of the trimer. (ox.ac.uk)
and monoclonal recombinant antibodies are also currently being pursued for prevention of HIV-1 infection in large Phase IIb clinical trials (NTC02716675 and NCT02568215). (frontiersin.org)

GP2D is the surface glycoprotein, secreted by infected cells, and SNP is the virus-associated GP. (lapizgrafico.com)

Type 1 fusion machines initially bind a receptor on the target cell surface, which triggers a conformational change, allowing for binding of the fusion protein. (wikipedia.org)

The tropism of the virus is determined by the SU protein domain because it is responsible for the receptor-binding function of the virus. (wikipedia.org)
The synthesized protein was incorrectly folded and incapable of binding CD4. (wikipedia.org)
Diskin and the group were able to distinguish the exact make-up of these antibodies - the way that one so-called "heavy chain" combined with another "light chain" - and to map the precise points at which these attached to the viral protein. (weizmann.ac.il)
Results: Biopanning of a melanocyte peptide phage-display library with alopecia areata patient sera, identified several novel melanocyte autoantigens, including OCA2-encoded P protein, the melanocortin 1 receptor (MC1R), and glycoprotein non-metastatic melanoma protein b. (bl.uk)
This antibody binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. (abcam.com)
The antibody also binds to P462L-substituted S318-510 fragments of the SARS spike protein. (abcam.com)
Precisely, this antibody binds to the 'open' conformation of the spike protein to the amino acids 318-510 in the S1 domain of the SARS-CoV as well as SARS-CoV-2 strains (32245784, Joyce et al. (abcam.com)
To further define which neurons express Eph, antibodies were generated to the cytoplasmic portion of the Eph protein. (sdbonline.org)
Sex hormone-binding globulin (SHBG) is the blood transport protein for testosterone and estradiol. (cdc.gov)
Our initial screen of MassBiologics' existing panel of SARS-CoVspecific antibodies resulted in Mabs with ELISA binding activity to the receptor binding domain of the SARS-CoV-2 Spike protein. (umassmed.edu)
Structure of a transmission blocking antibody in complex with Outer surface protein A from the Lyme disease spirochete, Borreliella burgdorferi. (umassmed.edu)
This review concentrates on the progress made toward understanding the role of this protein in MDR, identifying and characterizing the drug binding sites of Pglycoprotein, and modulating MDR by P-glycoprotein-specific inhibitors. (eurekaselect.com)
Furthermore, significant advances have been made in delineating the drug binding sites of this protein by studying mutant P-glycoprotein. (eurekaselect.com)
Photoaffinity labeling experiments and the use of site-directed antibodies to several domains of this protein have allowed the localization of the general binding domains of some of the cytotoxic agents and MDR modulators on P-glycoprotein. (eurekaselect.com)
Based on the available data, a topological model of P-glycoprotein and the approximate locations of its drug binding sites, as well as a proposed classification of multiple drug binding sites of this protein, is presented in this review. (eurekaselect.com)
IMPORTANCE The trimeric glycoprotein Env, the only viral protein expressed on the surface of HIV-1, is the target of broadly neutralizing antibodies and the focus of most vaccine development efforts. (stanford.edu)
The human integral membrane protein SERINC5 potently restricts HIV-1 infectivity and sensitizes the virus to antibody-mediated neutralization. (crick.ac.uk)
IgA autoantibodies in these patients have been shown to bind to desmogleins 1 and 3 in addition to an unspecified nondesmosomal transmembrane protein. (medscape.com)
Glycoprotein VI deficiency can be caused by mutations in the GP6 gene, which provides instructions for making a protein called glycoprotein VI (GPVI). (medlineplus.gov)
In response to an injury that causes bleeding, the GPVI protein begins clot formation by attaching (binding) to another protein called collagen that is found on blood vessel walls . (medlineplus.gov)
or a protein that is less able to bind to collagen. (medlineplus.gov)
As a result, there is a shortage (deficiency) of functional GPVI protein on the surface of platelets, which leads to bleeding problems characteristic of glycoprotein VI deficiency. (medlineplus.gov)
C-reactive protein,mannose-binding lectin, and serum amyloid P component activate complement and act as opsonins. (msdmanuals.com)

The following product was used in this experiment: Dystroglycan Polyclonal Antibody from Thermo Fisher Scientific, catalog # 11017-1-AP. (thermofisher.com)

HIV Vaccine Design to Target Germline Precursors of Glycan-Dependent Broadly Neutralizing Antibodies. (upenn.edu)
The first step was to understand exactly how and where the antibodies bind to the viral glycoprotein (part of a virus's outer membrane) used in the vaccine, and just how this binding neutralizes the virus so effectively. (weizmann.ac.il)
Previous SARS-CoV-2 infection substantially increased antibody responses after one vaccine and antibody and cellular responses remained 28-fold and 3-fold higher, respectively, after dual vaccination. (elifesciences.org)
Chiron Biocine examined an HSV-2 glycoprotein subunit vaccine including gD-2 and gB-2, which didn't prevent HSV disease but decreased intensity and length of recurrences (4, 29, 47). (bios-mep.info)
Broadly neutralizing HIV-1 antibodies (bNAbs) display protective potentials against experimental animal infection and thus are believed to be a key component of an effective HIV vaccine. (plos.org)
It is likely that a successful vaccine against HIV will need to stimulate the innate immune system, generate high levels of neutralising antibody, strong cellular immune responses, and mucosal immunity. (ox.ac.uk)
Our study revealed that a class of vaccine-induced human antibodies potently blocks HIV-1 Env-Galcer binding by perturbing the HIV-1 Env conformation. (duke.edu)
Elucidation of the structural correlates of a cross-linked viral glycoprotein will allow more rational use of this methodology for vaccine design, and reveals a strategy with promise for eliciting neutralizing antibodies needed for an effective HIV-1 vaccine. (ox.ac.uk)
Antibodies are a key component of the human adaptive immune system, and the elicitation of antibodies has been correlated with vaccine efficacy in many diseases ( 1 ). (frontiersin.org)

Receptor binding was only minimally affected, however, when the secreted env product was enzymatically deglycosylated. (wikipedia.org)
Further analysis of MC1R antibodies indicated that their binding sites were on extracellular domains of the receptor, were of the IgG1 subclass and, in two patients, adversely affected the function of the MC1R. (bl.uk)
At this time, there are five members within the IL-17 receptor family, and these are termed IL-17 RA, B, C, D and E. Not all receptors appear to bind known members of the IL-17 cytokine family. (rndsystems.com)
Fc receptor-dependent antibody functions are important components of the immune response that provide mechanisms for clearance of infected host cells, immune complexes, or opsonized pathogens. (frontiersin.org)
[ 13 ] The hallmark finding in IgA pemphigus is autoantibodies binding to sites containing the monocyte/granulocyte IgA-Fc receptor (CD89), causing intraepidermal neutrophilic pustules. (medscape.com)
Human complement receptor 2 (CR2) serves as a receptor for both C3dg and the gp350/220 glycoprotein of HERPESVIRUS 4, HUMAN, and binds the monoclonal antibody OKB7, which blocks binding of both ligands to the receptor. (bvsalud.org)

We describe two new mAbs reactive with the third domain of CD4 that inhibit steps subsequent to virus binding critical for HIV infectivity and cell fusion. (jenner.ac.uk)
Among the C1-specific MAbs that showed Galcer blocking, the antibody-dependent cellular cytotoxicity-mediating CH38 IgG and its natural IgA isotype were the most potent blocking antibodies. (duke.edu)
These particular mAbs bind to a portion of the Ebola virus's surface called the glycoprotein, which prevents the virus from entering a person's cells. (cdc.gov)

Cross-reaction was found between S. equina antigens and antibodies in the sera of Wuchereria bancrofti-infected patients, with the highest levels observed between sera of chronic infected patients and Setaria spp. (who.int)
Antibodies and antigens fit tightly together because their shape and other surface properties (eg, charge) are complementary. (msdmanuals.com)

The chimeric class I glycoprotein was expressed in target cells using an ALV derived retrovirus vector (RCASBP). (usda.gov)

One conserved antigenic site (amino acids 412 to 423) is disordered in the reported E2 structure, but a synthetic peptide mimicking this site forms a β-hairpin in complex with three independent NAbs. (ox.ac.uk)
We show here that one of the most conserved antigenic sites within the major glycoprotein E2 (amino acids 412 to 423), which is disordered in the recently reported crystal structure of an E2 core fragment, can adopt different conformations in the context of the infectious virus particle. (ox.ac.uk)
Similarities between the physical structures of avian and mammalian MHC class I glycoproteins have been proposed by comparative alignment of their amino acid sequences. (usda.gov)
Moreover, site-directed mutagenesis studies have identified the amino acids critical for the binding of some of these agents to P-glycoprotein. (eurekaselect.com)
Thus, among a series of vWF fragments, 125 I-bitiscetin only binds to those that overlap the A3 domain, ie, SpIII (amino acid [aa] 1-1365), SpI (aa 911-1365), and rvWF-A3 domain (aa 920-1111). (ashpublications.org)
they are called variable because the amino acids they contain are different in different antibodies. (msdmanuals.com)

The immunoreactivity of each of these potential autoantigens was tested in radioligand binding assays against 48 alopecia areata patient sera and was detected in 8 (16.7%), 21 (43.8%), and 10 (20.8%) patient serum samples, respectively. (bl.uk)
Scientific significance of serum anti- annexin V antibodies in Egyptian sufferers with scleroderma. (ncbcs.org)
In conclusion, measurement of serum anti-annexin V IgG antibodies in scleroderma sufferers could also be necessary for early prognosis of vascular and pulmonary issues. (ncbcs.org)
To address this, concentrations of testosterone, sex hormone binding globulin, androstanediol glucuronide (a metabolite of dihydrotestosterone) and estradiol were measured in stored serum specimens from men selected for the 1/3 subsample where organochlorine pesticide levels were determined. (cdc.gov)
Following HIV-1 infection, serum neutralizing antibody responses against the evolving autologous viral swarm are generated by the vast majority of infected subjects, usually within the first few months of infection [ 1 - 6 ]. (plos.org)
Here, by systematically building mutations into the hemagglutinin (H) glycoprotein of an attenuated measles virus strain and assaying for serum neutralization, we show that virus evolution is severely constrained by the existence of numerous co-dominant H glycoprotein antigenic sites, some critical for binding to the pathogenicity receptors SLAMF1 and nectin-4. (elsevierpure.com)
Lack of a substantial reduction in serum neutralization of mutant measles viruses that retain even one of the co-dominant antigenic sites makes evolution of pathogenic measles viruses capable of escaping serum neutralization in vaccinated individuals extremely unlikely. (elsevierpure.com)
2) The fraction of the blood serum containing antibodies. (mariamman.net)

however, many CRF01_AE viruses are resistant to neutralization mediated by these antibodies. (biomedcentral.com)
Our data may provide important information to understand the molecular mechanism regulating the neutralization susceptibility of CRF01_AE viruses to CD4bs antibodies. (biomedcentral.com)
Enhancing HIV-1 Neutralization by Increasing the Local Concentration of Membrane-Proximal External Region-Directed Broadly Neutralizing Antibodies. (stanford.edu)
However, binding of MPER-directed bNAb 10E8 to FcγRI abolishes the neutralization synergy that is seen with the N-heptad repeat (NHR)-targeting antibody D5_AR and NHR-targeting small molecule enfuvirtide (T20), possibly due to decreased accessibility of the NHR in the FcγRI-10E8-MPER complex. (stanford.edu)
Therefore, lipid binding may not be a strict requirement for potent neutralization by MPER-targeting bNAbs, as alternative methods can achieve similar increases in local concentrations while avoiding potential liabilities associated with immunologic host tolerance. (stanford.edu)
Broadly neutralizing antibodies targeting the membrane proximal external region (MPER) of Env show lipid-binding characteristics, and modulating this interaction affects neutralization. (stanford.edu)
In this study, we tested the neutralization potencies of variants of the MPER-targeting antibody 10E8 with different viral-membrane-binding and host FcγRI-binding capabilities. (stanford.edu)
Our results suggest that binding to both lipid and FcγRI improves the neutralization potency of MPER-directed antibodies by concentrating the antibodies at sites of viral fusion. (stanford.edu)

Priming HIV-1 broadly neutralizing antibody precursors in human Ig loci transgenic mice. (upenn.edu)

The localization of the binding site of bitiscetin within the A3 domain was further supported by showing that MoAb to vWF, which are specific for this domain and block the interaction between vWF and collagen, are potent inhibitors of the binding of bitiscetin to vWF and consequently of the bitiscetin-induced binding of vWF to platelets. (ashpublications.org)
The binding of GPVI to collagen also signals additional platelets to come together to increase the size of the clot. (medlineplus.gov)
Without GPVI binding to collagen, platelets cannot come together efficiently to form a clot, leading to the bleeding problems associated with glycoprotein VI deficiency. (medlineplus.gov)

Cellular receptors that bind the human immunodeficiency virus that causes AIDS. (harvard.edu)
In this review, we discuss the properties of Fc receptors, antibodies, and effector cells that influence ADCP. (frontiersin.org)

We have moved on to nanobody discovery for broadly neutralizing antibodies against all variants of concern and interest. (umassmed.edu)
Structural flexibility of a conserved antigenic region in hepatitis C virus glycoprotein E2 recognized by broadly neutralizing antibodies. (ox.ac.uk)
UNLABELLED: Neutralizing antibodies (NAbs) targeting glycoprotein E2 are important for the control of hepatitis C virus (HCV) infection. (ox.ac.uk)
IMPORTANCE: Approximately 180 million people worldwide are infected with hepatitis C virus (HCV), and neutralizing antibodies play an important role in controlling the replication of this major human pathogen. (ox.ac.uk)
Env-based immunogens tested so far in various animal species and humans have elicited binding and autologous neutralizing antibodies but not bNAbs (with a few notable exceptions). (plos.org)
Thus, our current understanding on how the expansion of particular B cell lineages by Env may be linked to the development of non-neutralizing antibodies is limited. (plos.org)
The two forms were equally immunogenic, but only the latter elicited neutralizing antibodies by stimulating a more restricted expansion of B cells to a narrower set of IGH/IGK/IGL-V genes that represented a small fraction (0.003-0.02%) of total B cells. (plos.org)
As such, lipid binding may not be uniquely required for MPER-targeting broadly neutralizing antibodies, as alternative methods to increase local concentration can achieve similar improvements in potency. (stanford.edu)

The presence of anti-phospholipid antibodies (aPL) have been outlined by our in-house ELISA strategies. (ncbcs.org)
Anti-annexin V antibodies have been measured with ELISA in 177 VOC and 81 steady-state SCA sufferers. (ncbcs.org)
Sera of 40 scleroderma sufferers and 15 wholesome controls have been examined for IgG and IgM anti-annexin V antibodies by ELISA and anticentromere antibodies by oblique immunofluorescence. (ncbcs.org)
Health care providers diagnose HIV infection by using two different types of antibody tests, ELISA and Western Blot. (digitalnaturopath.com)
Des préparations d'antigènes bruts de Setaria equina ont été utilisées dans le cadre des méthodes ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)

Thus, the DC-SIGN/E-glycoprotein interaction can be considered as an important target for inhibitors of viral replication. (unboundmedicine.com)
The binding of vWF to the latter can be initiated by high-shear conditions 1 that contribute to a shape change of the vWF molecule and to its interaction with GPIb. (ashpublications.org)

Left Plot) or Alexa Fluor® 647 Mouse Anti-Human CD361 antibody (Cat. (bdbiosciences.com)
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. (bdbiosciences.com)
The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. (bdbiosciences.com)

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 RC Monoclonal Antibody (Catalog # MAB22691, filled histogram) or isotype control antibody (Catalog # MAB0041 , open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab') 2 Secondary Antibody (Catalog # F0101B ). (rndsystems.com)

Broadly neutralizing HIV-1 antibodies (bNAbs) isolated from infected subjects display protective potential in animal models. (plos.org)
Taken together, our results suggest that lipid-binding activity and FcγRI-mediated potentiation function in concert to improve the potency of MPER-directed bNAbs by increasing their local concentration near the site of viral fusion. (stanford.edu)

1. Homburger H, Larsen S: Detection of specific antibodies. (mariamman.net)

We investigated the prevalence of anti-annexin V IgG and IgM antibodies in sera of scleroderma sufferers and their relation to the presence of different antibodies and improvement of illness morbidity. (ncbcs.org)

Because early HIV infection often causes no symptoms, a doctor usually diagnoses it by testing a person's blood for the presence of antibodies to HIV. (digitalnaturopath.com)
HIV antibodies generally do not reach detectable levels in the blood for one to three months following infection. (digitalnaturopath.com)
In 10-30% of infected subjects, antibodies capable of neutralizing not only the autologous virus but also heterologous viruses are generated, usually following several years of infection [ 2 , 5 , 7 - 13 ]. (plos.org)

In immunogenicity studies, the neutralizing antibody response to cross-linked trimers showed modest but significantly greater breadth against a global panel of difficult-to-neutralize Tier-2 heterologous viruses. (ox.ac.uk)

Whatever the initial stimulus, the final common event is a cross-linking of platelets by binding of fibrinogen to a membrane-binding site, glycoprotein IIb/IIIa (GPIIb/IIIa). (justia.com)
On resting platelets, GP IIb/IIIa is unable to bind fibrinogen or vWf. (medscape.com)

Tissue, cells or virus corresponding to Human coronavirus SARS-CoV-2 Spike Glycoprotein S1. (abcam.com)
Induction of apoptosis by cross-linking antibodies certain to human B-lymphoma cells: expression of Annexin V binding websites on the antibody cap. (ncbcs.org)
Human and mouse CD229 exhibit cross-species binding (7). (rndsystems.com)
The mention of any product names or non-United States Government entities on CDC Ebola websites is not meant to serve as an official endorsement of any such product or entity by the CDC, the Department of Health and Human Service, or the United States Government. (cdc.gov)

Several mutations have been found to alter the GP binding site residues. (lapizgrafico.com)
A lipid binding groove and clusters of conserved residues highlight potential functional sites. (crick.ac.uk)

However, in addition to the problems of generating neutralising antibodies and mucosal immunity, the difficulty of inducing broad cellular responses able to protect against all clades of HIV, remains an important issue. (ox.ac.uk)

The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). (novusbio.com)
Based on studies looking at exon deletions, a key ligand-binding site would appear to exist over aa 425‑441 (13). (rndsystems.com)

mostly by employing serologic methodologies and monoclonal antibody isolation and characterization. (plos.org)

The glycoprotein spike complex of the Ebola virus bound by a neutralizing antibody isolated from a vaccinated individual. (weizmann-usa.org)

Furthermore, equilibrium binding assays using plasma membranes from MDR cells and radioactive drugs have aided our understanding of the modes of drug interactions with P-glycoprotein. (eurekaselect.com)
A cryo-EM structure of CH235 UCA bound to Man5-enriched CH505.N279K.G458Y.SOSIP.664 revealed interactions of the antibody light chain complementarity determining region 3 (CDR L3) with the engineered Env loops D and V5. (ox.ac.uk)

HSV-1 glycoprotein gE binds the IgG Fc site and inhibits C1q binding and antibody-dependent mobile cytotoxicity (ADCC) (Fig. (bios-mep.info)

Based on the observation that, in contrast to the inhibition of HIV-1 entry, antibody mediated enhancement was not temperature dependent and could not be mediated by F(ab) fragments, we concluded that the mechanisms underlying these phenomena are different and that antibody mediated enhancement of HIV-1 entry is largely if not exclusively mediated by HIV-1 glycoprotein cross-linking. (microbiologyresearch.org)

The second drug, Ebanga™ , is a single monoclonal antibody and was approved in December 2020. (cdc.gov)

Specific ablation of PDGFRbeta-overexpressing pericytes with antibody-drug conjugate potently inhibits pathologic ocular neovascularization in mouse models. (stanford.edu)

An isotype control should be used at the same concentration as the antibody of interest. (bdbiosciences.com)

Please refer to primary antibody product webpage for recommended antibody dilution. (cellsignal.com)

We tested whether an antibody-drug conjugate (ADC) - an engineered monoclonal antibody linked to a cytotoxic agent - could selectively ablate pericytes and suppress retinal and choroidal neovascularization.Methods: Immunoblotting, flow cytometry, cell viability test, and confocal microscopy were conducted to assess the internalization and cytotoxic effect of ADC targeting mPDGFRbeta in an in vitro setting. (stanford.edu)

Antibodies can exert their protective functions via a multitude of mechanisms. (frontiersin.org)

Anatomy20

Organisms19

Diseases1

Chemicals and Drugs112

Analytical, Diagnostic and Therapeutic Techniques and Equipment46

Phenomena and Processes62

Disciplines and Occupations2

Information Science4

Health Care4

Monoclonal Antibodies17

Type I transmembrane glycoprotein3

Gp12019

Epitopes8

Transmembrane glycoprotein1

Envelope18

Antigen13

Specificity2

Affinity12

Proteins10

Epitope4

Platelet3

Recombinant6

Surface glycoprotein1

Conformational1

Protein23

Polyclonal Antibody1

Vaccine9

Receptor6

Mabs3

ANTIGENS2

Chimeric1

Amino6

Serum8

Neutralization8

Broadly neutrali1

Collagen3

Receptors2

Neutralizing Antibodies8

ELISA5

Interaction2

Alexa Fluor3

Catalog1

BNAbs2

Specific antibodies1

Different antibodies1

Infection3

Trimers1

Fibrinogen2

Human4

Residues2

Immunity1

Ligand bind2

Characterization1

Ebola1

Interactions2

Cytotoxicity1

Fragments1

20201

Potently1

Isotype1

Dilution1

Conjugate1

Protective1