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  • workflow
  • Wu and colleagues (2014) have published an improved mass spectrometry (MS) workflow with parallel data acquisition for efficient, sensitive identification and sequencing of intact glycopeptides. (thermofisher.com)
  • 1 Pulling together the enhanced specifications of the new Orbitrap Fusion Tribrid mass spectrometer from Thermo Scientific, the researchers present a product-dependent, parallel data-acquisition and data-analysis workflow that reduces false positives, in addition to accurately identifying the glycopeptide backbone. (thermofisher.com)
  • The kit is compatible with our lysis, reduction, alkylation and digestion reagents to provide a complete workflow for studying sialylated glycopeptides. (thermofisher.com)
  • systematic name
  • Endo-α-N-acetylgalactosaminidase (EC 3.2.1.97, endo-α-acetylgalactosaminidase, endo-α-N-acetyl-D-galactosaminidase, mucinaminylserine mucinaminidase, D-galactosyl-3-(N-acetyl-α-D-galactosaminyl)-L-serine mucinaminohydrolase, endo-α-GalNAc-ase, D-galactosyl-N-acetyl-α-D-galactosamine D-galactosyl-N-acetyl-galactosaminohydrolase) is an enzyme with systematic name glycopeptide-D-galactosyl-N-acetyl-α-D-galactosamine D-galactosyl-N-acetyl-galactosaminohydrolase. (wikipedia.org)
  • The systematic name of this enzyme class is N-linked-glycopeptide-(N-acetyl-beta-D-glucosaminyl)-L-asparagine amidohydrolase. (wikipedia.org)
  • amino
  • Amphipathic glycopeptides, the amino acid sequence of which comprises an N-terminal opioid message sequence, a C-terminal helical address sequence, and a linker sequence between the message sequence and the helical address sequence, where the C-terminal helical address sequence has a length of nine amino acids, and at least one of the amino acid residues of the peptide is glycosylated. (freepatentsonline.com)
  • PNGaseF can for N -linked glycopeptides), and those glycosidases that eliminate certain, well specified sugar structures leave an unmodified amino acid and thus no trace of the previous modification site. (mcponline.org)
  • Unlike standard SPPS (which is limited to 50 amino acid residue) NCL allows the construction of large glycopeptides. (wikipedia.org)
  • Proteomics
  • 2013) " Sweet-Heart: An integrated suite of enabling computational tools for automated MS2/MS3 sequencing and identification of glycopeptides ," Journal of Proteomics, 84 (pp. 1-16). (thermofisher.com)
  • hydrophilic
  • This approach ensures all sizes of glycopeptides are enriched for analysis and compared against the most commonly used zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC)-based strategy. (thermofisher.com)
  • These include the following: solid phase derivatization, enhanced ionization of sialoglycopeptides, differentiation of sialic acid linkage, and enrichment of the modified glycopeptides by hydrophilic interaction liquid chromatography. (springer.com)
  • ions
  • They found that HCD was better for matching glycopeptide identities than ETD, because the technique produced greater quantities of glyco-oxonium ions for onward analysis in the CID/ETD MS/MS step. (thermofisher.com)
  • negatively
  • 1 The high selectivity of TiO 2 toward negatively charged sialylated glycopeptides results in highly specific binding of these species. (thermofisher.com)
  • Sialyl glycopeptides can be positively, negatively, or neutrally charged depending on pH of their buffer solution and ionization conditions. (springer.com)
  • enzyme
  • Other names in common use include: glycopeptide N-glycosidase, glycopeptidase, N-oligosaccharide glycopeptidase, N-glycanase, Jack-bean glycopeptidase, PNGase A, and PNGase F The enzyme uses a catalytic triad of cysteine-histidine-aspartate in its active site for hydrolysis by covalent catalysis. (wikipedia.org)
  • neutral
  • Our results for neat glycopeptides corroborate the observation that the glycopeptides present in the Venn diagram for TiO 2 were N -linked and acidic, while unique glycopeptides in ZIC-HILIC were mainly N -linked and neutral. (thermofisher.com)
  • To detect sialoglycopeptides, a negative-ion mode mass spectrometry may be applied with a minimal loss of sialic acids, although the positively charged or neutral glycopeptides may be excluded. (springer.com)