• HMGB1, as well as lysine acetylation and cysteine disulfide oxidation of secreted HMGB1, were monitored by sensitive and specific assays using immunoprecipitation, stable isotope dilution, differential alkylation, and nano liquid chromatography parallel reaction monitoring/high-resolution mass spectrometry (nano-LC-PRM/HRMS). (bvsalud.org)
  • Two totally different silylating reagents, HSi[OCH(CF 3 ) 2 ] 3 and MTBSTFA, are instrumental for the profitable implementation of this strategy, getting used for the activation and transient masking of unprotected amino acids and peptides at C -termini and N -termini, respectively. (n-egt.com)