Spent coffee grounds were treated to obtain suitable carriers for both physical and covalent immobilization of Candida rugosa lipase. (uniroma1.it)
Rugosa1
In the present study, immobilisation of Candida rugosa (CRL) lipase onto amino-activated mica is performed via covalent bonding (namely Amino-CRL) and the cross-linking of lipases into nano-reactors through physical adsorption (namely NER-CRL). (researchgate.net)
Enzymes5
Other enzymes and lipases from other microorganisms were also tested in the biocatalytic esterification of mono-and disaccharides. (researchgate.net)
In 1993, Monsan returned to INSA Toulouse to lead a research group focusing on the discovery, characterisation and molecular engineering of enzymes, including glucansucrases and lipases. (wikipedia.org)
His fundamental includes investigation of structure-activity relationships of enzymes, (particularly glycoside hydrolases and lipases), and enzyme discovery by functional metagenomics. (wikipedia.org)
The results have indicated the nature and concentration of the divalent cations solutions have played a significant role and strongly affected the amount and the enzymatic activity of the immobilized enzymes. (unesp.br)
For the free and immobilized system, the enzymes retained 32% and 92% of their initial activities, respectively, at the end of 48 days of storage. (gazi.edu.tr)
Enzyme3
The optimized immobilized biocatalyst obtained by adsorption mechanism showed up to 33 U∙g-1 of immobilized enzyme activity, and a higher pH, thermal, and storage stability than the free lipase. (uniroma1.it)
The zeolitic materials were used as a solid supports for the immobilization of fungal Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL) lipases and the catalytic potential of these nanozeolite-enzyme complexes were explored in the transesterification reactions of oil palm oil and microalgae aiming the biodiesel production for biodiesel production using ethanol as solvent. (unesp.br)
The best catalytic performance were obtained with the TLL enzyme immobilized on Nano-X supports ion exchanged with nickel sulfate 0.5 mol.L-1 (Nano-X/Ni/0.5M-TLL). (unesp.br)
Physical adsorption1
In the first case, physical adsorption was directly performed after water/organic solvent extraction of the interferents from the support, while in the second case the delignification and the activation of the support with glutaraldehyde or KIO4 were necessary. (uniroma1.it)
Immobilisation1
Immobilisation d'enzymes à l'aide de glutaraldehyde (PhD Thesis). (wikipedia.org)
Synthesis1
Extending this work, immobilised lipases have novel capabilities in the synthesis of sugar esters. (researchgate.net)
The temperature range generally oscillates between 40 °C and 60 °C, a characteristic interval of the immobilized form, which usually guarantees better conditions because it is more stable than the free form. (researchgate.net)
C, a characteristic interval of the immobilized form, which usually guarantees better conditions because it is more stable than the free form. (researchgate.net)
Method2
Each immobilization method was investigated to obtain the best insoluble biocatalyst immobilized activity, efficiency, and recovery. (uniroma1.it)
A series of gelatin hydrogels were prepared by crosslinking method using glutaraldehyde (GA). The hydrogels were characterized by gel formation, swelling/degradation tests, and FTIR analysis. (gazi.edu.tr)
Catalytic3
Till date, studies that investigated the effect of glutaraldehyde concentration on catalytic efficacy of biocatalyst developed with silica-derived from oil palm leaves (OPL) as support, are unknown. (ugm.ac.id)
Herein, the effect of glutaraldehyde concentration on the catalytic efficacy of immobilized CRL was assessed by the enzymatic production of butyl butyrate as a model. (ugm.ac.id)
Glutaraldehyde is widely used for lipase immobilization while the appropriate strategy optimizes the catalytic performance of lipase. (mysciencework.com)
Chitosan1
In this paper, the A. fumigatus α-amylase had been immobilized onto zeolite/chitosan hybrid to improve its thermal-stabilization for industrial needs. (ijournalse.org)
Silica2
Fourier transform infrared (FTIR) spectra and immobilization parameters indicated that covalent bound CRL on functionalized OPL-derived silica-magnetite composite activated with 4% (v/v) glutaraldehyde solution (100 mM, pH 7.0) (CRL/G/A/SiO 2 -M) and pretreated in toluene, resulted in a protein loading and an immobilization yield of 68.3 mg/g and 74.3%, respectively. (ugm.ac.id)
20 ] immobilized laccase on magnetic mesoporous silica microspheres and used it to treat coking wastewater. (researchsquare.com)
Immobilization1
An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes. (ijournalse.org)
Zeolite1
21 ] immobilized laccase on zeolite (NZ) - graphene oxide (GO) composite nanoparticles as a carrier to prepare a novel nano biocatalyst for degradation of Direct Red 23 organic pollutants. (researchsquare.com)
Activity1
The results showed that the activity of immobilized laccase by two-step method (2977.5 U/g) was much higher than that by one-step method (239.5 U/g). (researchsquare.com)