Oxidation of glucoseElectrodeRecombinant glucose oxidaseEnzymesBiosensorAspergillusAmperometricCatalyzes the oxidationCatalase positiveElectrodesDehydrogenaseHydrogen peroxideDeterminationPlasma glucoseReagentMeasurementCovalentHexokinaseYeastPeroxidaseEnzymatic glucoseReactiveZinc oxideOxygenBindsGlycoproteinSpecificityConcentration of glucoseInsulinFree glucoseEnzyme activityMedical diagnosisProteinColloidalOxidize glucoseProteinsStabilityLactateImmobilizationReactionElectrocatalyticSerumImpaired glucose tShelf lifeCyclicDiabetesGlycogenBacteriaCarbonBloodMetabolismLevelsMETHODGlycolysisSensitivity
Oxidation of glucose5
- The glucose oxidase enzyme (GOx or GOD) also known as notatin (EC number 1.1.3.4) is an oxidoreductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. (wikipedia.org)
- It is a flavoprotein, which catalyses the oxidation of glucose. (biovencer.com)
- Economical oxidation of glucose is achieved by oxidation with an immobilized glucose oxidase-catalase composition in a reaction mixture at low temperatures below 15.degree. (justia.com)
- In this process, the enzyme mixture (GO-CAT) effects an oxidation of glucose into glucono-.delta. (justia.com)
- The resulting new material has significant catalytic activity in the electrochemical oxidation of glucose. (eurekalert.org)
Electrode15
- Enzymatic glucose biosensors use an electrode instead of O2 to take up the electrons needed to oxidize glucose and produce an electronic current in proportion to glucose concentration. (wikipedia.org)
- In the present research, a new biocompatible electrode is proposed as a rapid and direct glucose biosensing technique that improves on the deficiencies of fast clinical devices in laboratory investigations. (frontiersin.org)
- A combined electrocatalytic approach using chloroperoxidase and a glucose oxidase electrode has been developed for the measurement of chlorophenol (CP) congeners. (canada.ca)
- Together with glucose oxidase immobilized on a working glassy carbon electrode (+0.45 V vs. Ag/AgCl), chlorinated oxidation products have been demonstrated as efficient mediators in a glucose oxidase/glucose system. (canada.ca)
- The direct electron transfer (DET) between glucose oxidase (GOD) and the underlying glassy carbon electrode (GCE) can be readily achieved via colloidal laponite nanoparticles as immobilization matrix. (univ-grenoble-alpes.fr)
- Based on the decrease of oxygen electrocatalytic signal, the proposed laponite/GOD/GCE was successfully applied in the reagentless glucose sensing at -0.45 V. The proposed electrode exhibited fast amperometric response (8 s), broad linear range (2.0 × 10-5-1.9 × 10-3 M), good sensitivity (4.8 ± 0.5 mA M-1 cm-2), low detection limit (1.0 × 10-5 M) at a signal-to-noise ratio of 3, and excellent selectivity. (univ-grenoble-alpes.fr)
- In this study, construction of amperometric glucose biosensor was carried out by immobilizing of glucose oxidase (GOD) on carbon paste electrode (CPE) which was coated with polyaniline (PANI) by cyclic voltammetry technique. (dergipark.org.tr)
- Since the current values to be measured depending on the glucose concentration will be affected by the graphite:mineral oil composition of the electrode, this parametres were optimized by response surface methodology (RSM). (dergipark.org.tr)
- A novel electrochemical glucose biosensor based on a poly (L-aspartic acid)-modified carbon-paste electrode. (dergipark.org.tr)
- Glucose biosensor based on immobilization of glucose oxidase in poly(o-aminophenol) film on polypyrrole-Pt nanocomposite modified glassy carbon electrode. (dergipark.org.tr)
- Reagentless glucose biosensor based on direct electron transfer of glucose oxidase immobilized on colloidal gold modified carbon paste electrode. (dergipark.org.tr)
- Glucose biosensor based on immobilization of glucose oxidase in electrochemically polymerized polytyramine film and overoxidised polypyrrole film on platinized carbon paste electrode. (dergipark.org.tr)
- Amperometric Glucose Biosensor based on homopolymer-chitosan double layered glucose oxidase electrode modified with zinc oxide nanoparticles. (dergipark.org.tr)
- A glucose biosensor based on glucose oxidase enzyme and ZnO nanoparticles modified carbon paste electrode. (dergipark.org.tr)
- A biosensor for determination of glucosinolates has been fabricated by employing a bienzyme system composed of myrosinase and glucose oxidase immobilized onto an eggshell membrane and a dissolved oxygen electrode as the transducer. (edu.hk)
Recombinant glucose oxidase2
- In silico and in vivo analysis of signal peptides effect on recombinant glucose oxidase production in nonconventional yeast Yarrowia lipolytica. (bvsalud.org)
- The aim of this study was in silico and in vivo analysis of SPs effect on the production of recombinant glucose oxidase (GOX) in Yarrowia lipolytica . (bvsalud.org)
Enzymes5
- Home / Enzymes / Glucose oxidase from Aspergillussp. (innovativeenzymes.com)
- Every Glucose oxidase enzyme manufacturer across the country knows the immense importance of the enzyme in various industries and most manufacturers are not able to keep up with it,unlike Ultreze enzymes. (ultrezenzymes.com)
- Ultreze enzyme is one glucose oxidase enzyme manufacturer that has steady supplies and major contribution in all these areas and industries and has globally delivered quality enzymes at every stage, making them the current best enzyme manufacturer in the country. (ultrezenzymes.com)
- Netherlands-based Enzymes supplier DSM has developed a new glucose oxidase for bakeries to improve the stability of products during fermentation. (bakeryandsnacks.com)
- These are enzymes glucose oxidase or glucose dehydrogenase, which are produced by microorganisms. (eurekalert.org)
Biosensor2
- The electrochemical behavior of glucose on the fabricated GOx/nano-ZnO/PVA/FTO biosensor was investigated by I - V techniques. (frontiersin.org)
- Using response surface methodology for amperometric glucose biosensor construction. (dergipark.org.tr)
Aspergillus1
- Glucose oxidase enzyme is a widely used enzyme obtained from a particular fungal strain of aspergillus niger. (ultrezenzymes.com)
Amperometric1
- Among the most successful and reliable diagnostic devices are the glucose electrochemical biosensors that determine the glucose blood level based on glucose oxidase (GOx) using amperometric, impedimetric, conductometric, and potentiometric techniques. (frontiersin.org)
Catalyzes the oxidation2
- Glucose oxidase catalyzes the oxidation of β-D-glucose into D-glucono-1,5-lactone, which then hydrolyzes into gluconic acid. (wikipedia.org)
- Bilirubin oxidase (BOD) is a multicopper oxidase that catalyzes the oxidation of bilirubin to biliverdin. (sigmaaldrich.com)
Catalase positive1
Electrodes3
- Glucose biosensing at carbon paste electrodes containing iron nanoparticles. (dergipark.org.tr)
- Glucose oxidase-polypyrrole electrodes synthesized in p-toluenesulfonic acid and sodium p-toluenesulfonate. (dergipark.org.tr)
- Such electrodes will more accurately determine the concentration of glucose in the blood. (eurekalert.org)
Dehydrogenase1
- Glucose dehydrogenase from Pseudomonas sp. (sigmaaldrich.com)
Hydrogen peroxide5
- Several species of fungi and insects synthesize glucose oxidase, which produces hydrogen peroxide, which kills bacteria. (wikipedia.org)
- Early experiments showed that notatin exhibits in vitro antibacterial activity (in the presence of glucose) due to hydrogen peroxide formation. (wikipedia.org)
- citation needed] In the glucose oxidase assay, the glucose is first oxidized, catalyzed by glucose oxidase, to produce gluconate and hydrogen peroxide. (wikipedia.org)
- 1. Role of glucose oxidase in wheat flour Glucose oxidase can be converted into glucose by the presence of oxygen in the presence of oxygen, and hydrogen peroxide. (enzymes.bio)
- Oxidative stress in skin of B6C3F1 mice of both sexes was generated by intradermal injection of the hydrogen peroxide (H2O2) -producing enzyme, glucose oxidase with polyethylene glycol (GOD+PEG). (cdc.gov)
Determination6
- Glucose oxidase is widely used for the determination of free glucose in body fluids (medical testing), in vegetal raw material, and in the food industry. (wikipedia.org)
- Glucose oxidase is widely used coupled to peroxidase reaction that visualizes colorimetrically the formed H2O2, for the determination of free glucose in sera or blood plasma for diagnostics, using spectrometric assays manually or with automated procedures, and even point-of-use rapid assays. (wikipedia.org)
- During recent decades, the determination of blood glucose level has become essential and routine because of the rapid increase in the incidence of diabetes mellitus and associated diseases. (frontiersin.org)
- GO has also found wide use as an analytical auxiliary, for example, for glucose determination. (justia.com)
- Scientists at the Ural Federal University (UrFU Russia) have solved the problem of synthesizing enzymefree electrocatalytic sensors for quantitative determination of glucose. (eurekalert.org)
- Blood glucose determination: plasma or serum? (medscape.com)
Plasma glucose6
- Blood samles were assayed for plasma glucose, insulin, growth hormone, adrenaline and noradernaline in the fasting state and 10, 30, 60, 90, 120 and 180 minutes after the end of smoking by the glucose oxidase method for glucose, radioimmunoassay for insulin and growth hormone, and flourometry for catecholamines. (who.int)
- The results of the present study showed significant elevation of plasma glucose after both types of smoking, with peak glucose response occurring earlier after Goza smoking. (who.int)
- Diabetes was defined by the presence of fasting plasma glucose 126 mg/dl or the use of oral hypoglycemic agents or insulin. (bvsalud.org)
- Serum glucose values are 1.15% lower than plasma glucose values. (medscape.com)
- Morning fasting plasma glucose values are higher than afternoon glucose levels. (medscape.com)
- In heparinized plasma, glucose concentrations are 5% lower than plasma. (medscape.com)
Reagent2
- Grow a fresh culture (18 to 24 hours) of bacteria on nutrient agar The stability of glucose oxidase reagent is excellent and refrigeration during the course of analyzer is unnecessary (10). (cappartnersfund.com)
- 2. Role of glucose oxidase in medical diagnosis A reagent for blood sugar content measurement. (enzymes.bio)
Measurement1
- Last but not least, exogenous interfering substances may cause errors in the systematic measurement of blood glucose. (frontiersin.org)
Covalent1
- Spin-coated polyvinyl alcohol (PVA) at optimized instrumental deposition conditions was applied to prepare the effective medium for glucose oxidase enzyme (GOx) covalent immobilization through cyanuric chloride (GOx/nano-ZnO/PVA/FTO). (frontiersin.org)
Hexokinase1
- Finally, the method is compared with automated glucose oxidase and hexokinase procedures. (cappartnersfund.com)
Yeast1
- Widespread bread making techniques include the use of high gluten wheat flour mixed with eggs, water, yeast, and glucose oxidase to bring toughness to the dough. (ultrezenzymes.com)
Peroxidase1
- Sociodemographic and diagnosis variables (presence of dental calculus and periodontal status) were considered to measure salivary concentrations of glucose (by the glucose oxidase/peroxidase method, amylase (by the colorimetric test), urea (by the amount of indophenol), total protein (by the Bradford method) and albumin (by the nephelometric method). (bvsalud.org)
Enzymatic glucose1
- Since immobilized (carrier fixed) enzyme preparations, and among these also immobilized GO and CAT, have been for years part of the state of the art, the idea of using immobilized preparations also for enzymatic glucose oxidation appears to be obvious. (justia.com)
Reactive1
- The effects of EGCG on glucose oxidase (GO)-induced reactive oxygen species (ROS) production was determined using the ROS probe CM-H 2 DCFDA. (molvis.org)
Zinc oxide1
- It was developed using an indium gallium zinc oxide (IGZO) transistor and glucose oxidase. (news-medical.net)
Oxygen4
- This enzyme is produced by certain species of fungi and insects and displays antibacterial activity when oxygen and glucose are present. (wikipedia.org)
- It is also used as a food preservative to help remove oxygen and glucose from food when packaged such as dry egg powder to prevent unwanted browning and undesired taste. (wikipedia.org)
- Glucose oxidase plays an immensely important role here by providing the boon of de-oxygenation which helps maintain oxygen levels during these processes. (ultrezenzymes.com)
- Industrial use of soluble GO-CAT mixtures has already been made for a long time by the food industry for removal of glucose or oxygen traces from food or beverages. (justia.com)
Binds3
- The active site where glucose binds is in a deep pocket. (wikipedia.org)
- The glucose oxidase binds specifically to β-D-glucopyranose and does not act on α-D-glucose. (wikipedia.org)
- Glucose binds to glycogen phosphorylase and competitively inhibits the enzyme. (cappartnersfund.com)
Glycoprotein1
- In this study, we analyze the size distribution and structure of N-linked oligosaccharides attached to the recombinant glycoprotein glucose oxidase (GOD) and the cell wall mannoproteins obtained from H. polymorpha. (unboundmedicine.com)
Specificity1
- Diagnosis of Cerebrospinal Fluid Rhinorrhea: Lack of Specificity of the Glucose Oxidase Test Tape. (stanford.edu)
Concentration of glucose2
- The absorbance at 505 nm is proportional to concentration of glucose in the sample. (wikipedia.org)
- Thus, scientists are faced with the task of synthesizing enzyme-free catalysts, which will make it possible to more accurately and with lower financial costs determine the concentration of glucose in the blood. (eurekalert.org)
Insulin2
- ERO1-beta, a pancreas-specific disulfide oxidase, promotes insulin biogenesis and glucose homeostasis. (nih.gov)
- Of the 10 GLUT receptors, GLUT-4 receptors are present in muscle and adipose tissues and require insulin for glucose transport. (medscape.com)
Free glucose2
- Because free glucose is the product of the hepatic glucose-6-phosphatase reaction, either type leads to accumulation of liver glycogen, accompanied by fasting hypoglycemia . (medscape.com)
- The liver loses its capacity as a glucose-homeostatic organ because of a fundamental inability to release free glucose. (medscape.com)
Enzyme activity1
- [ 1 ] Two of the patients had almost total deficiency of hepatic glucose-6-phosphatase, whereas the remaining 4 had normal enzyme activity. (medscape.com)
Medical diagnosis1
- Glucose oxidase enzyme finds its way into the medical sector and is heavily used in a certain glucose monitoring device used on humans to aid proper medical diagnosis. (ultrezenzymes.com)
Protein3
- The main body of this project has been dedicated to investigating the effect of key formulation parameters, such as pH, ionic strength and temperature, on the stability and the aggregation mechanism of a model protein, Glucose oxidase at low (1 mg/ml) protein concentration. (bl.uk)
- In the production of noodles, glucose oxidase is a good protein network structure, which can increase the strength of the noodles. (enzymes.bio)
- The immobilized glucose oxidase-catalase composition preferably contains glucose oxidase and catalase bonded to hardened protein particles with the catalase activity being at least one-sixth of the glucose oxidase activity. (justia.com)
Colloidal1
- Colloidal laponite nanoparticles: Extended application in direct electrochemistry of glucose oxidase and reagentless glucose biosensing. (univ-grenoble-alpes.fr)
Oxidize glucose1
- It is known to oxidize glucose enzymatically in aerated agitator reactors by means of an enzyme mixture consisting of glucose oxidase (GO) and catalase (CAT). (justia.com)
Proteins1
- The discovery of leptin more than a decade ago established a paradigm in which secreted proteins from adipocytes coordinate energy balance and glucose homeostasis ( 2 , 3 ). (diabetesjournals.org)
Stability2
- When glucose oxidase was immobilized with spacers of different lengths, it was found that storage stability decreased with increasing the length of the spacer. (gazi.edu.tr)
- Possible substitution options include palladium (it has stability and increased sensitivity to glucose). (eurekalert.org)
Lactate2
- Glucose limitation provides several important advantages, including control of the glucose metabolism, reduced lactate concentration, better medium utilization, and simple perfusion control. (cappartnersfund.com)
- However, glucose-6-phosphate is also the substrate for glycolysis and produces lactate. (medscape.com)
Immobilization2
- Evidence from systematic experiments was gathered for the case of immobilization of an inorganic catalyst (zerovalent iron-Fe0) and a biocatalyst (glucose oxidase -GOx) on textile support. (diva-portal.org)
- Glucose biosensors based on the immobilization of copper oxide and glucose oxidase within a carbon paste matrix. (dergipark.org.tr)
Reaction1
- It oxidises all of the glucose in solution because the equilibrium between the α and β anomers is driven towards the β side as it is consumed in the reaction. (wikipedia.org)
Electrocatalytic1
- The new system, the developers say, can be used to create an enzyme-free electrocatalytic sensor for determining glucose in whole blood. (eurekalert.org)
Serum3
- This is the technology behind the disposable glucose sensor strips used by diabetics to monitor serum glucose levels. (wikipedia.org)
- This enzyme is widely used in making biosensors to detect the glucose levels in blood or serum. (biovencer.com)
- For serum glucose, a red-top tube can be used. (medscape.com)
Impaired glucose t1
- Objective: To assess the prevalence of DM and impaired glucose tolerance (pre-diabetes) among newly diagnosed tuberculosis cases in Ijebu-Ode Local Government Area of Ogun State, Nigeria. (bvsalud.org)
Shelf life2
- When compared among the other methods of de-oxygenation, Glucose Oxidase took the top position by providing the best quality oxidative metamorphism and helped keep the food fresher for longer along with significantly increasing their shelf life. (ultrezenzymes.com)
- In short Glucose oxidase prevents the beer from losing its flavour, delays its ageing and gives it its impressive shelf life! (ultrezenzymes.com)
Cyclic1
- At pH 7, glucose exists in solution in cyclic hemiacetal form as 63.6% β-D-glucopyranose and 36.4% α-D-glucopyranose, the proportion of linear and furanose form being negligible. (wikipedia.org)
Diabetes6
- Some routine clinical laboratory checks for glucose levels, which have become an integral part of diabetes care, suffer from several limitations in accuracy, such as strip manufacturing variation, strip storage, and aging. (frontiersin.org)
- Compared with fingerstick blood glucose monitoring, CGM has many advantages in diabetes management. (cappartnersfund.com)
- Every Glucose oxidase enzyme manufacturer knows the value it holds in the battle against diabetes and would suffer immense goodwill loss upon not maintaining stable supply. (ultrezenzymes.com)
- This ocular prototype can detect glucose levels, making it extremely useful to monitor people with diabetes . (news-medical.net)
- Glucose measurements are used in the diagnosis and treatment of pancreatic islet cell carcinoma and of carbohydrate metabolism disorders, including diabetes mellitus, neonatal hypoglycemia, and idiopathic hypoglycemia. (cdc.gov)
- For gestational diabetes testing, parameters are 1 hour after 50 g of glucose and 2 hours after 100 g of glucose. (medscape.com)
Glycogen2
- Glucose is primarily stored as glycogen in muscles and liver. (medscape.com)
- In the hepatocyte, the glycogen catabolic machinery normally responds to stimuli caused by hypoglycemia (eg, neural, hormonal), ending in a flood of glucose-6-phosphate that cannot be released from the cell. (medscape.com)
Bacteria1
- Glucose Oxidase--Natural enzyme slows growth of mouth bacteria. (arcatapet.com)
Carbon1
- They use sunlight as an energy source to turn carbon dioxide into glucose. (naturalnews.com)
Blood12
- Ingested glucose is absorbed directly into the blood from the intestine and results in a rapid increase in blood glucose. (cappartnersfund.com)
- Glucose oxidase reacts directly to the glucose present in the blood, giving comparatively better results. (ultrezenzymes.com)
- Deviations would only occur when glucose oxidase reacts with the blood glucose. (ultrezenzymes.com)
- Other similar systems are either noticeably less sensitive to glucose, or operate in an alkaline environment and provide for an additional stage of dilution of blood samples, in certain cases a thousand times. (eurekalert.org)
- This increases the sensitivity of the blood glucose test. (eurekalert.org)
- The patients were selected from four health facilities and were screened for elevated Fasting Blood Glucose levels using a laboratory spectrophotometer (glucose oxidase method). (bvsalud.org)
- Eleven (11.0%) had elevated blood glucose levels suggestive of DM, while 20 (20.0%) had impaired fasting blood glucose levels. (bvsalud.org)
- For fasting glucose testing, collect the blood sample in the morning after an overnight or 8-hour fast. (medscape.com)
- For postprandial glucose testing, collect the blood sample 2 hours after a regular meal. (medscape.com)
- For oral glucose tolerance testing, after oral intake of 75 g of glucose, collect blood samples at 1 hour and 2 hours. (medscape.com)
- The usual homeostatic mechanism cannot halt the rapid drop in blood glucose levels that normally occurs during the first several hours after birth (reflecting consumption of maternal glucose), and the decrease continues. (medscape.com)
- This decrease in circulating glucose can be precipitous, resulting in no measurable blood level. (medscape.com)
Metabolism2
- Glucose monitoring is essential to evaluate the degree of glucose metabolism disorders. (cappartnersfund.com)
- Thus, during aerobic metabolism, each molecule of glucose can generate 36 ATPs. (medscape.com)
Levels3
- Similar assays allows the monitoring of glucose levels in fermentation, bioreactors, and to control glucose in vegetal raw material and food products. (wikipedia.org)
- over a period to identify patterns and peaks in glucose levels. (cappartnersfund.com)
- Hyperuricemia can reach levels that require use of xanthine oxidase inhibitors to prevent nephrolithiasis. (medscape.com)
METHOD2
- These include, but are not limited to, the filter paper test, direct plate method, swab method, impregnated oxidase test strip method and test tube method. (cappartnersfund.com)
- This invention relates to a novel particle-bound, water-insoluble glucose oxidase-catalase composition having a definite minimum catalase activity, as well as to a method of using the same for glucose oxidation at low temperatures. (justia.com)
Glycolysis1
- Glucose is initially used by glycolysis and is converted to pyruvate. (medscape.com)
Sensitivity1
- The I - V results indicated good sensitivity for glucose detection (0.041 mA per mM) within 0.2-20 mM and the limit of detection was 2.0 μM. (frontiersin.org)