The assays include ATP and ADP binding to myosin and actin.myosin, displacement of ADP from myosin and actin.myosin, and the cleavage of ATP to ADP and phosphate on myosin. (bvsalud.org)
Rabbit2
Full length native protein (purified) corresponding to Rabbit Fast Myosin Skeletal Heavy chain. (abcam.cn)
Synthetic peptide corresponding to Human heavy chain Myosin/MYH3 aa 100-200 conjugated to keyhole limpet haemocyanin. (abcam.com)
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). (abcam.cn)
S-1 contains the heads of the heavy chains plus the light chains and S-2 contains part of the double-stranded, alpha-helical, heavy chain tail (myosin rod). (lookformedical.com)
Due to the high binding-affinity of GA with myosin heavy chain (MYH), GA@Fe3O4-NH2 exhibits excellent adsorption performance towards myosin. (bvsalud.org)
Protein2
The HIU pretreatment provided more opportunities for EGCG grafting onto the ultrasound-treated protein (UMP) by disrupting the filamentous polymerization of myosin and thus further facilitated MP dissolution. (bvsalud.org)
For practical application, myosin is successfully isolated from the pig left ventricular protein extract with GA@Fe3O4-NH2, and SDS-PAGE and LC-MS/MS showed that myosin had high purity and a total of 716 proteins could be identified. (bvsalud.org)
Muscle1
Calcium-dependent activation of the thin filament mediated by the troponin-tropomyosin complex is key in the regulation of actin-myosin based muscle contraction. (bvsalud.org)
Human1
Perturbations to this system, either physiological (e.g., phosphorylation of myosin light chains) or pathological (e.g., mutations that cause familial cardiomyopathies), can alter calcium sensitivity and thus have important implications in human health and disease. (bvsalud.org)