Glial cells cultures. Medical search. Frequent questions

The cilium, however, only has the nine pairs of peripheral microtubules while it lacks the axial pair of microtubules, making its structure very similar to the cilia of neurons, Schwann cells, and astrocytes of the CNS. (wikipedia.org)
Satellite glial cells in sensory ganglia are laminar cells that wrap around sensory neurons. (wikipedia.org)
Some sensory neurons have small projections called microvilli that extend outward from their cell surfaces. (wikipedia.org)
In the sympathetic ganglia, satellite glial cells are one of three main types of cells, the other two being the sympathetic ganglion neurons and small intensely fluorescent (SIF) cells. (wikipedia.org)
The purpose of this study was to investigate PDT using the photosensitiser, meta tetrahydroxyphenyl chlorin (mTHPC), on rat neurons and satellite glia, compared with human adenocarcinoma cell (MCF-7). (open.ac.uk)
Conclusion: Neurons in culture can survive mTHPC-PDT under conditions sufficient to kill tumour cells and other nervous system cells. (open.ac.uk)
It is encoded by gene drb1 that is expressed in neurons, not in glial cells. (nih.gov)
Induction of long-term potentiation (LTP) of the synaptic connection between two hippocampal glutamatergic neurons in a neural network formed in cell culture resulted in a specific pattern of potentiation at other connections within the network. (jneurosci.org)
In cultured hippocampal slices, LTP induced at one set of synaptic inputs to CA1 pyramidal neurons also spreads to nearby synapses on the same postsynaptic neuron ( Engert and Bonhoeffer, 1997 ), resulting in a "breakdown" of input specificity. (jneurosci.org)
In cultures of dissociated hippocampal neurons, induction of long-term depression (LTD) at glutamatergic synapses is accompanied by a backpropagation of depression to input synapses on the dendrites of the presynaptic neuron. (jneurosci.org)
In the present study, we used cultures of dissociated hippocampal neurons to characterize the spread of LTP in defined neural networks. (jneurosci.org)
Using perforated whole-cell patch clamp, we simultaneously monitored all synaptic connections within the networks of three or four neurons. (jneurosci.org)
For these studies we use neurons that are maintained in cell culture as well as genetically-modified strains of mice that are at increased risk for developing neurodegenerative conditions analogous to those observed in humans. (uml.edu)
With whole-cell recording from single neurons, we found that ACh inhibited excitatory postsynaptic currents (EPSCs) while elevating neuronal firing in a dose-dependent manner. (springer.com)
Previously, we have demonstrated that small networks of cultured hippocampal neurons exhibit persistent activity in response to brief stimuli applied to single cells [ 26 ], reminiscent of reverberatory activity in the hypothetical cell assembly proposed by Donald Hebb as a network basis of "online" working memory [ 27 ]. (springer.com)
Neuregulin expressed by neurons binds the receptor tyrosine kinase erbB2 on Schwann cell microvilli that directly contact the axon. (jneurosci.org)
Primary cultures of cortical neurons and astrocytes were treated with EO (0-25%) for up to 90 min. (hindawi.com)
Primary cultures of neocortical neurons were obtained from 16-day-old NMRI mouse embryos (Charles River, Iffa Credo, Saint-Germain-sur-l'Arbresle, France) [ 5 , 6 ]. (hindawi.com)
The cell suspension for astrocyte culture was obtained in the same way as for neurons. (hindawi.com)
These findings support that Nurr1 exerts a protective effect on adult mDA neurons in a cell-autonomous manner Indeed, several intrinsic mechanisms associated with Nurr1-mediated cell survival have been identified. (justia.com)
Cell 137: 47-59) has identified an unexpected effect of Nurr1 which is expressed in glial cells in response to stimuli which trigger inflammation, and this Nurr1 suppresses the production of pro-inflammatory cytokines that cause the death of mDA neurons, but does not suggest a therapeutic effect through induction of Nurr1 expression. (justia.com)
In particular, glial cells in nerve tissues include astrocytes and microglial cells and are accessory cells that aid in promoting the functions and survival of neurons. (justia.com)
However, under a pathologic condition, the glial cells are instead activated to set up an environment in which the glial cells cause damage to neurons (M1 activation). (justia.com)
Meanwhile, it is known that glial cells with an M1 phenotype which set up a diseased environment can be converted into glial cells with an M2 phenotype which promote a therapeutic environment in which the survival and regeneration of neurons is set up. (justia.com)
Consequently, the present research looked into whether SHH could be changed by purmorphamine within the transdifferentiation of Mller glial cells to retinal neurons, and therefore, attempted to give a far more convenient, stabilized and effective therapy. (exposed-skin-care.net)
To study the interaction of neurons with CNS glial cells, dissociated sympathetic or sensory ganglion cells or fetal retinal cells were plated onto cultures of dissociated optic nerve glial cells of young rats. (nih.gov)
Isolated myelin from adult rat spinal cord was adsorbed to polylysine-coated culture dishes and tested as a substrate for peripheral neurons, neuroblastoma cells, or 3T3 cells. (nih.gov)
Expression of nucleoside diphosphate kinase, mitochondria-associated adenylate kinase, and several mitochondria-associated creatine kinase isozymes was highest in the outer retina, whereas expression of cytosolic adenylate kinase and brain creatine kinase was higher in the cones, horizontal cells, and amacrine cells indicating the diversity of ATP-buffering strategies among retinal neurons. (molvis.org)
Mixed-cell aggregate cultures of fetal rat telencephalon were used as in vitro model, as well as aggregates enriched either in neurons or in glial cells. (unige.ch)
We demonstrate that neural stem/progenitor cell (NSPC) self-renewal and spatiotemporal generation of neurons and other cell types are severely impacted by the loss of Ythdf2 in embryonic neocortex. (biomedcentral.com)
The neurotropic JHM strain of mouse hepatitis virus (JHMV) replicates primarily within glial cells following intracranial inoculation of susceptible mice, with relative sparing of neurons. (escholarship.org)
We are having troubles with contamination in our primary cell cultures of neurons. (cellculturedish.com)
For culturing neurons we use Neurobasal-A (Gibco 10888-022) supplemented with 1% antibiotics/antimycotic (Gibco 15240-062) and for culturing glia cells, we use DMEM. (cellculturedish.com)
Survival of neurons and particularly retinal cells will be evaluated on NCD diamond surfaces. (europa.eu)
by building up artificial retinal cell interfaces using stimulation devices and by tuning the biocompatibility of the stimulation electrodes and their surface interactions with neurons, we proposed to fabricate microelectrodes and microelectrode arrays (MEAs) as well as ion sensitive field effect transistors (ISFETs) for medical applications in vivo. (europa.eu)
Studies include analysis of disease progression in mouse models of Alzheimer's disease (AD) and multiple sclerosis (MS), in vitro studies using primary cultures of neurons and glial cells from wild type and transgenic mice, and analysis of the molecular events involved. (uic.edu)
Double-staining studies with specific cell markers to distinguish astrocytes, neurons and oligodendrocytes were performed. (lu.se)
COMT immunoreactivity colocalized with a specific oligodendrocyte marker galactocerebroside in cells displaying oligodendrocyte morphology, flat cells displaying type-1 astrocyte morphology and glial fibrillary acidic protein, in branched cells displaying type-2 astrocyte morphology and in cell bodies of neurons, the processes of which displayed neurofilament immunoreactivity. (lu.se)
The results suggest that COMT is synthesized by cultured astrocytes, oligodendrocytes and neurons. (lu.se)
We also use primary retinal cells to investigate the effects of nanowires on different cell types, in particular neurons compared to glial cells. (lu.se)
Transmission electron microscopy revealed intranuclear herpetic inclusions, and immunostaining revealed HuAHV1 and herpesvirus particles in neurons, glial cells, tongue mucosal epithelium, and hepatocytes. (cdc.gov)
Huntington disease (HD) is an incurable, adult-onset, autosomal dominant inherited disorder associated with cell loss within a specific subset of neurons in the basal ganglia and cortex. (medscape.com)

It is known that astrocytes (a major type of glial cells) can release classical neurotransmitters and modulate the excitability of neuronal ensembles. (imperial.ac.uk)
Therefore, one of the most basic yet essential open questions about neuro-glial circuits, and about cortical computation more generally, is the following: How do astrocytes respond to natural sensory stimuli in awake behaving animals? (imperial.ac.uk)
The team measured the effects of two widely used statins, simvastatin and pravastatin, on glial progenitor cells, which can become either astrocytes or oligodendrocytes. (sciencedaily.com)
Won-suk Chung , a postdoc in in Ben Barres' lab at Stanford School of Medicine, Palo Alto, California, studies astrocytes-the most common glial cell in the brain. (alzforum.org)
Using confocal microscopy, the researchers saw astrocytes engulfing axon terminals and synapses on these retinal cells. (alzforum.org)
Unlike wild-type cells, astrocytes in the MEGF10- and MERTK-deficient mice ingested no synaptic components and the visual system of these animals failed to mature, suggesting that phagocytosis of synapses contributes to development of the retina. (alzforum.org)
Astrocytes are specialized glial cells ubiquitously distributed throughout the spinal cord and brain. (news-medical.net)
Astrocytes are a heterogeneous population of cells with distinguishing functional and morphological characteristics and are specialized to their different brain regions and locations. (news-medical.net)
8 Astrocytes interact with blood vessels, pericytes, and epithelial cells to organize CNS flow and modulate the blood-brain barrier (BBB). (news-medical.net)
Rat primary astrocytes were isolated from different brain regions (cortex, cerebellum, and hippocampus) and grown in vitro to demonstrate the utilization of live-cell analysis for monitoring and comparing the diversity of cultured astroglia phenotypes. (news-medical.net)
We find that microglia phagocytose more AβpH than astrocytes in culture, in brain slices and in vivo. (ucl.ac.uk)
Previous studies have shown the contribution of glial cells such as astrocytes in TDP-43-linked ALS. (nature.com)
Neuroinflammation, a striking and common hallmark involved in many neurodegenerative diseases, including ALS, is characterized by extensive activation of glial cells including microglia, astrocytes and oligodendrocytes. (nature.com)

Subsequently, the cells were transferred to fresh culture medium, without purmorphamine or SHH-N, for a further 2 days to investigate Mller glia-derived cell differentiation. (exposed-skin-care.net)
Previous studies have indicated that newly formed oligodendrocytes are dynamic cells whose production, survival, and differentiation depend upon axonal influences. (rupress.org)
In this study, it was observed that the ion implantation stimulated the neural proliferation and the implantation of different ions on cell culture surfaces was essential to determine the effects of this technique on adhesion, proliferation, differentiation and apoptosis properties of cells in details. (omicsonline.org)

The expression of NSPC and astrocyte markers nestin and Glial fibrillary acidic protein (GFAP), respectively, was up- and down-regulated, respectively. (nih.gov)

Scientists found that both compounds, when used at doses that mimic those that patients take, spur glial progenitor cells to develop into oligodendrocytes. (sciencedaily.com)
For example, in one experiment, they found about five times as many oligodendrocytes in cultures of human progenitor cells exposed to pravastatin compared to cultures not exposed to the substance. (sciencedaily.com)
differentiated oligodendrocytes were nonpermissive for cell adhesion, neurite growth, or fibroblast spreading. (nih.gov)
These nonpermissive oligodendrocytes were characterized by a radial, highly branched process network, often contained myelin basic protein, and may, therefore, correspond to cells actively involved in the production of myelin-like membranes. (nih.gov)
Oligodendrocytes, the myelin-forming cell in the central nervous system (CNS) 1 , are neuroepithelial in origin. (rupress.org)

Satellite glial cells, formerly called amphicytes, are glial cells that cover the surface of neuron cell bodies in ganglia of the peripheral nervous system. (wikipedia.org)
In some cases, neuron survival was confirmed by measuring subsequent neurite growth in monolayer culture. (open.ac.uk)
Importantly, 4 µg ml -1 mTHPC PDT caused no significant neuron death compared with untreated controls but was sufficient to elicit substantial cell death in the other cell types. (open.ac.uk)
The protocol was validated using hypericin (0-3 µg ml -1 ), which caused neuron death equivalent to other cell types. (open.ac.uk)
We found that potentiation propagated from the site of induction retrogradely to glutamatergic or GABAergic synapses received by the dendrites of the presynaptic neuron and laterally to those made by its axonal collaterals onto other glutamatergic cells. (jneurosci.org)
In contrast, synapses made by the same presynaptic neuron onto GABAergic cells were not affected, and there was no postsynaptic lateral or forward propagation to other synapses received or made by the postsynaptic neuron. (jneurosci.org)
We model Lewy body disorders in mice and rats in vivo and in primary neuron and glial cell cultures in vitro. (duq.edu)
Furthermore, SHH-treated cells had been shifted to neural lineage by expressing neuron-specific course III -tubulin (Tuj1), directing cell destiny to pole Cbz-B3A cells (14). (exposed-skin-care.net)
General physicochemical properties of myelin were not responsible for this effect, since myelin from rat sciatic nerves favored neuron adhesion and neurite growth as well as spreading of 3T3 cells. (nih.gov)

Both satellite glial cells (SGCs) and Schwann cells (the cells that ensheathe some nerve fibers in the PNS) are derived from the neural crest of the embryo during development. (wikipedia.org)
Satellite glial cells are a type of glia found in the peripheral nervous system, specifically in sensory, sympathetic, and parasympathetic ganglia. (wikipedia.org)
Despite their flattened shape, satellite glial cells contain all common organelles necessary to make cellular products and to maintain the homeostatic environment of the cell. (wikipedia.org)

Beneficial and deleterious impact of nutritional supplementation for inhibition of proliferation of neuroblastoma in culture. (uml.edu)
Microtubule-Severing ATPase Spastin in Glioblastoma: Increased Expression in Human Glioblastoma Cell Lines and Inverse Roles in Cell Motility and Proliferation. (uib.no)
Cytosine arabinoside (AraC) 10 μ M was added to the media the last 2 days to prevent proliferation of other glial cells. (hindawi.com)
Following 2 days of culture, cells on the coverslips were fixed in 4% paraformaldehyde at 4C for 10 min and processed for immunocytochemistry to detect proliferation-associated markers. (exposed-skin-care.net)
Changing surface characteristics of scaffolds was a way to promote regeneration and ion implantation was one of the methods to modify surface properties which play a huge role in enhancing the proliferation and integration of cells. (omicsonline.org)
We investigate immortalized cells in terms of migration, proliferation, stress and membrane conformation on the nanowires. (lu.se)

Other scientists outlined new approaches to promote a kind of glial cell activation that might help mice tackle Alzheimer's-like pathology in the brain (see part two of this series). (alzforum.org)
As described on their poster-and reported 24 November in Nature-they first examined neuronal-glial interactions in mice less than a week old. (alzforum.org)
NEW YORK, September 21, 2003 - New research from Memorial Sloan Kettering Cancer Center (MSKCC), Cornell University, and The University of Connecticut describes a novel way of producing therapeutic nerve cells that can cure mice with Parkinson's-like disease. (mskcc.org)
IFN-gamma-mediated control of JHMV replication is dampened in glial cultures derived from the neural progenitor cells of type I receptor knock-out mice. (escholarship.org)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
[ 7 ] More recent data from striatal neuronal cultures transfected with mutant huntingtin and transgenic mice carrying the spinocerebellar ataxia-1 (SCA-1) gene (another CAG repeat disorder) suggest that NIIs may not be necessary or sufficient to cause neuronal cell death, but translocation into the nucleus is sufficient to cause neuronal cell death. (medscape.com)

Two groups from University College London, led by Alison Lloyd and Martin Raff, report in ScienceExpress today that glial cells and oligodendrocyte precursor cells, both removed from the central nervous system of young rats, can divide indefinitely--in one case for more than 20 months--if the culture medium contains or lacks certain ingredients, such as hormones. (scientificamerican.com)

Dual polarization of microglia isolated from mixed glial cell cultures. (ox.ac.uk)
Microglia are versatile immune effector cells of the CNS and are sensitive to various stimuli. (ox.ac.uk)
The influence of cell sorting methods on the polarization state of microglia has never been studied. (ox.ac.uk)
Mixed glial culture system (MGCS) and magnetic activated cell sorting (MACS) are two methods that are commonly used to purify microglia. (ox.ac.uk)
To understand the mechanisms by which n-3/n-6 PUFA imbalance affects CNS development, we investigated the impact of maternal dietary n-3 PUFA deficiency on offspring's microglia, the resident immune cells involved in CNS development and homeostasis 11 . (nature.com)
Microglia are efficient phagocytes of synaptic material and apoptotic cells, which are key processes in the developing brain 12 . (nature.com)
Taken together, our results reveal a previously unrecognized non-cell-autonomous mechanism in TDP-43-mediated neurodegeneration, identifying COX-2-PGE2 as the molecular events of microglia- but not astrocyte-initiated neurotoxicity and identifying celecoxib as a novel potential therapy for TDP-43-linked ALS and possibly other types of ALS. (nature.com)

We have developed a human Aβ_{1-42} analogue (Aβ^{pH}) that exhibits green fluorescence upon internalization into the acidic organelles of cells but is non-fluorescent at physiological pH. (ucl.ac.uk)

Western blots detected both soluble 24 kDa and membrane-bound 28-kDa COMT proteins in neuronal and astrocyte cultures. (lu.se)
Following up on earlier work that showed MWCNT exposure impaired the blood-brain barrier and promoted neuroinflammatory glial responses, we treated primary astrocyte cultures with the exosomal fraction. (cdc.gov)

RBM45 predominantly localizes in cytoplasm of cultured cells and specifically binds to poly(C) RNA. (nih.gov)
These two LC PUFAs are found predominantly in the form of phospholipids and constitute the building blocks of brain cell membranes 1 . (nature.com)
Previous biochemical and histochemical studies have suggested that catechol-O-methyltransferase (COMT) is a predominantly glial enzyme in the brain. (lu.se)

In glial cells after hypoxia/reoxygenation, the iNOS mRNA and protein expressions were detected by reverse-transcription polymerase chain reaction and by immunocytochemical analysis, respectively. (elsevierpure.com)
Age-dependent association between protein expression of the embryonic stem cell marker Cripto-1 and survival of glioblastoma patients. (uib.no)
C1q is a lectin-like protein that binds apoptotic cells. (alzforum.org)
Our goals were twofold: 1) to characterize the gene expression, protein expression, and activity of key synthesizing and regulating enzymes of energy metabolism in the whole mouse retina, retinal compartments, and/or cells and 2) to provide an integrative analysis of the results related to function. (molvis.org)
Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:12441351). (qedbio.com)
TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, L-selectin, and alpha-cleavage of amyloid protein precursor (APP). (qedbio.com)
Using a neuronal model in which aSyn cell-autonomous toxicity is quantitatively scored by longitudinal survival analysis, we found that stabilization of 14-3-3 protein-proteins interactions with Fusicoccin-A (FC-A) decreases aSyn-dependent toxicity. (bvsalud.org)

Earlier work in the lab had revealed that the star-shaped cells express high levels of phagocytic receptors. (alzforum.org)
Impaired phagocytic function of glial cells during later stages of AD likely contributes to worsened disease outcome, but the underlying mechanisms of how this occurs remain unknown. (ucl.ac.uk)

This study demonstrates that glial cells derived from neural progenitor cells are susceptible to JHMV infection and that treatment of infected cells with IFN-gamma inhibits viral replication in a dose-dependent manner. (escholarship.org)
These data indicate that JHMV is capable of infecting glial cells generated from neural progenitor cells and that IFN-gamma-mediated control of viral replication is dependent, in part, on type I IFN secretion. (escholarship.org)

Wallerian degeneration includes a characteristic demyelinating response of Schwann cells surrounding the distal stump of an injured peripheral nerve. (jneurosci.org)
Physicians are looking at statins as a possible treatment for multiple sclerosis, where the myelin coating that covers nerve cells in the central nervous system is damaged. (sciencedaily.com)
In 2001, Lorenz Studer, MD , Head of the Stem Cell and Tumor Biology Laboratory at MSKCC, and his colleagues at Rockefeller University published research in which they generated unlimited numbers of genetically matched dopamine nerve cells using cloned stem cells whose genetic material originated from the mouse's own tail. (mskcc.org)

Spana and Doe, 1996 ) (mediated by Notch and Delta and cell-cell interaction), are involved in the specification of daughter cell fates. (biologists.com)

About half of the cells in the brain are glial cells. (imperial.ac.uk)
This knowledge, however, is based almost entirely on work performed either in culture or in brain slices. (imperial.ac.uk)
Cholesterol-lowering drugs known as statins have a profound effect on an elite group of cells known as glial progenitor cells that are important to brain health as we age, scientists have found. (sciencedaily.com)
Neuroscientists found that statins, one of the most widely prescribed classes of medication ever used, have an unexpected effect on brain cells. (sciencedaily.com)
Researchers looked at the effects of statins on glial progenitor cells, which help the brain stay healthy by serving as a crucial reservoir of cells that the brain can customize depending on its needs. (sciencedaily.com)
The new research published in the July issue of the journal Glia by Steven Goldman, M.D., Ph.D., and first author Fraser Sim, Ph.D., provides direct evidence for an effect of statins on brain cells. (sciencedaily.com)
These findings were made through experiments done in cell culture using human brain cells and exposing them to doses of statins used widely in patients. (sciencedaily.com)
In this study, Sim ran a genomic screen to see which genes are more active in these cells compared to other brain cells. (sciencedaily.com)
The team looked at progenitor cells from 16 patients who had brain tissue removed during surgery to treat epilepsy, tumors, or vascular problems. (sciencedaily.com)
As the brain ages, those functions seem to get creaky, raising the possibility that keeping glial cells young and fit could protect against neurodegeneration. (alzforum.org)
This study aimed at elucidating the effect of major non-THC phytocannabinoids on the fate of adult neural stem progenitor cells (NSPCs), which are an essential component of brain function in health as well as in pathology. (nih.gov)
Temporal monitoring of brain region astroglia revealed differences in cell growth and morphology. (news-medical.net)
Phagocytosis by glial cells is essential to regulate brain function during health and disease. (ucl.ac.uk)
Dr Feinstein showed that DMF reduces inflammation in the brain, and recently reported that DMF increases the number of T regulatory cells that reduce CNS inflammation. (uic.edu)
COMT immunoreactivity was studied in primary astrocytic cultures from newborn rat cerebral cortex, and in neuronal cultures from rat brain from 18-day-old rat embryos using antisera against rat recombinant COMT made in guinea pig. (lu.se)

In the Drosophila central nervous system (CNS) glial cells are known to be generated from glioblasts, which produce exclusively glia or neuroglioblasts that bifurcate to produce both neuronal and glial sublineages. (biologists.com)
We show that the genesis of a subset of glial cells, the subperineurial glia (SPGs), involves a new mechanism and requires Notch. (biologists.com)

Based on the antibody intensities and the COX and LDH activity, Müller glial cells (MGCs) had the lowest capacity for glycolysis, aerobic glycolysis, and OXPHOS. (molvis.org)

Neurofilaments Form Helical, Flexible Bundles During Neuritogenesis in Culture and Mature Axons in Situ. (uml.edu)
Instead, it is a site where axons project from neuronal cell bodies in the supraoptic and paraventricular nuclei of the hypothalamus. (medscape.com)
These hypothalamic cell bodies produce hormones that undergo axonal transport through the pituitary stalk and into terminal axons within the neurohypophysis. (medscape.com)

The aim of this work was to study its localization and molecular forms in primary cultures, where cell types can be easily distinguished with specific markers. (lu.se)

An abscess forms when an area of cerebral inflammation becomes necrotic and encapsulated by glial cells and fibroblasts. (msdmanuals.com)

We want to understand how the "glial code" resembles the neuronal code and how it differs from it and, most importantly, how they work together to support cortical computation. (imperial.ac.uk)
Images show cultures at 30-40% confluence (2 days, cortical, or 4 days, hippocampal and cerebellar). (news-medical.net)

Although human HSCs as vehicles to treat metachromatic leukodystrophy (MLD) has been used to treat patients with early onset MLD in a phase I/II trial, the HSCs give rise to all different blood cell lineages, such as the myeloid and lymphoid cell lineages [ 11 ]. (hindawi.com)
The generation of the embryonic CNS is a lineage-based process in which neural progenitors, called neuroblasts (NBs), give rise to largely invariant lineages of neural/glial cells. (biologists.com)
Cell lineage analysis techniques have been used to analyse most of the embryonic NB lineages at the histological level. (biologists.com)

These large cells appear, under hematoxylin and eosin staining, to have pale abundant cytoplasm and pale nuclei with finely dispersed chromatin. (medscape.com)

The team found that the compounds spur the cells, which are very similar to stem cells, to shed their flexibility and become one particular type of cell. (sciencedaily.com)
Goldman's team is recognized as a leader identifying and directing the molecular signals that direct the development of stem cells and their daughter cells, known as progenitor cells. (sciencedaily.com)
Stem cells. (nih.gov)
Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)
Indeed, the potent pathotropic migratory properties of BMSCs and ability to circumvent both the complications associated with immune rejection of allogenic cells and many of the moral reasons associated with embryonic stem cell use suggest that BMSCs are most promising stem cells as a potential target for the clinical use of genetically engineered stem cells [ 14 , 15 ]. (hindawi.com)
Sokullu E, Dağcı T, Gözen O, Ersoy F, Öztarhan A (2017) The Effects of Carbon and Gold Ion Implanted Surfaces on Neuronal Stem Cells' Functions. (omicsonline.org)
Using this surface modification method, it was aimed to improve surface characteristics and achieve a bioactive surface for neural stem cells. (omicsonline.org)
Even though the integration of stem cells was promising, neural stem cell studies still have many milestones to reach. (omicsonline.org)

Cells were grown for 2 weeks in DMEM : F12 containing 10% fetal bovine serum (FBS) until reaching confluence. (hindawi.com)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

Female Sprague-Dawley rats implanted with bone cancer cells develop mechanical and thermal hyperalgesia but antagonizing GM-CSF in these animals significantly reduced such hypersensitivity. (iasp-pain.org)

Therefore, the present inventors have found that various neurologic disorders based on inflammation may be treated since transcription factors Nurr1 and Foxa2 interact with each other to convert glial cells from an M1 phenotype to an M2 phenotype which sets up a therapeutic environment, when the transcription factors are overexpressed in the glial cells. (justia.com)

3 H]Flunitrazepam and [ 3 H]TBOB binding, [ 3 H]GABA uptake, cell viability, and morphology were assayed. (hindawi.com)
This allowed us to image, for the first time, glial uptake of Aβ^{pH} in real time in live animals. (ucl.ac.uk)

Specifically, a method of converting glial cells from an M1 phenotype to an M2 phenotype, wherein Nurr1 and Foxa2 are introduced into the glial cells to be overexpressed in the glial cells and a method of preventing or treating an inflammatory neurologic disorder, which includes glial cells into which Nurr1 and Foxa2 are introduced, or a viral vector loaded with Nurr1 and Foxa2, are provided. (justia.com)
To this aim, we use STED microscopy, phase holography microscopy, flow cytometry, immunocytochemistry and electron microscopy techniques to investigate the phenotype of a variety of cells cultured on nanowires and nanotubes. (lu.se)

Proinsulin C-peptide is known to bind specifically to cell membranes and to exert intracellular effects, but whether it is internalized in target cells is unknown. (researchgate.net)

The levels of nitrite produced in the cultured media of glial cells significantly increased after 12-h hypoxia but not after 0- and 6-h hypoxia. (elsevierpure.com)
Production of pro-inflammatory cytokines TNFα and IL-1β by H4 and U118-MG cells occurred under basal conditions but infection of the cells with HSV-1 did not significantly upregulate production. (iospress.com)
The number, diversity, and complexity of astroglia cells have increased significantly with evolution, and research from several groups implies that gender differences exist. (news-medical.net)

This mechanism of specifying glial cell fates within the CNS is novel and provides further insight into regulatory interactions leading to glial cell fate determination. (biologists.com)
We strive for a detailed understanding of the interactions between cells and nanostructures with respect to cell behavior, cell physiology and cell mechanics. (lu.se)
Our goal is to understand in detail the interactions between cells and nanostructures such as nanowires and nanotubes with respect to cell behavior, cell physiology and cell mechanics. (lu.se)
In order to deepen our understanding of the interactions between the cell membrane and nanostructures, we perform STED microscopy live imaging of the membrane of cells cultured on nanostructures. (lu.se)

These results suggested that the endogenous neurogenic capacity of retinal Mller glial cells could be improved by this little molecular agonist from the SHH signaling pathway. (exposed-skin-care.net)

Mebiianism of neuronal cell death in Alzheimer disease. (nii.ac.jp)

There is much more to be learned about these cells, and research surrounding additional properties and roles of the SGCs is ongoing. (wikipedia.org)
At the Society for Neuroscience (SfN) annual meeting, which drew nearly 25,000 scientists to San Diego November 9-14, researchers reported new data on the fundamental roles played by glial cells in forming and refining neural circuits. (alzforum.org)
Ionic forces between cells and material and Van der Walls forces which make short term physicochemical bonding have active roles during this phase. (omicsonline.org)

Based on a previous report, we studied the potential involvement of the adenosine A1 receptor in the effect of CBC on these cells and found that the selective adenosine A1 receptor antagonist, DPCPX, counteracted both ERK1/2 phosphorylation and up-regulation of nestin by CBC, indicating that also adenosine is involved in these effects of CBC, but possibly not in CBC inhibitory effect on GFAP expression. (nih.gov)

This degeneration requires close contact of the distal axon with Schwann cells, but what is the signal? (jneurosci.org)
Phospho-specific erbB2 antibodies detected activated erbB2 at nodal and paranodal regions of myelinating Schwann cells. (jneurosci.org)
it also blocked neuregulin-induced demyelination of Schwann cells in vitro when added to the glial compartment of a cell culture chamber. (jneurosci.org)

Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
The use of real-time, live-cell analysis provides new possibilities and cell biology workflows. (news-medical.net)

CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

Rat and mouse cells do not feature such a "countdown" mechanism, and researchers from London have now shown that at least some types of rat cells from the nervous system can divide virtually forever without turning cancerous. (scientificamerican.com)
Mediates the proteolytic cleavage and shedding of FCGR3A upon NK cell stimulation, a mechanism that allows for increased NK cell motility and detachment from opsonized target cells. (qedbio.com)

SIF cells of sympathetic ganglia are separated into groups, each of which is surrounded by an SGC sheath. (wikipedia.org)

abstract = "Nitric oxide (NO) produced by inducible nitric oxide synthase (iNOS) exerts inhibitory and cytotoxic effects on various cells including neuronal cells. (elsevierpure.com)

The Cecal Appendix: One More Immune Component with a Function Disturbed by Post-Industrial Culture. (evolutionnews.org)

Retinal ganglion cells (RGCs) develop in waves, starting at the central retina and then progressing outward toward the periphery. (jneurosci.org)
At this young age, retinal ganglion cells shed synapses as a normal part of visual system development. (alzforum.org)
NBs divide in an asymmetric manner to bud off a set of ganglion mother cells (GMC), which in turn divide once to produce two postmitotic daughters. (biologists.com)

Here, we have used cocultures of neuroglioma (H4) and glioblastoma (U118-MG) cells as a minimal in vitro model to investigate whether Aβ is produced by neuroglioma cells and whether this could result in protective anti-viral activity against HSV-1 infection. (iospress.com)
Our data established that H4 neuroglioma cells produced Aβ42 in response to HSV-1 infection thus inhibiting secondary replication. (iospress.com)
Survey experience is sufficient to conclude that laboratory aerosol infection does not occur in the course of routine work with cell cultures and animals not subject to chronic infection. (cdc.gov)

New insights into cell-specific and compartmental ATP and GTP production, as well as utilization and buffering strategies and their relationship with known retinal and cellular functions, are discussed. (molvis.org)
The Incucyte ® Live-Cell Analysis System delivers flexible kinetic quantification of biological phenotypes to monitor growth, motility, morphology, and cellular function. (news-medical.net)
this limitation has been overcome via ectopic expression of human telomerase reverse transcriptase (hTERT), the catalytic component of telomerase, to produce large quantities of these cells as an attractive source for cellular transplantation [ 16 - 18 ]. (hindawi.com)
We measure cellular forces in cells and tissues with high spatial resolution using arrays of vertical nanowires. (lu.se)

In some SGCs of the sensory ganglia researchers have seen a single cilium that extends outward from the cell surface near the nucleus and into the extracellular space of a deep indentation in the plasma membrane. (wikipedia.org)
Due to their close proximity to the SGC sheath, these microvilli of the neuronal plasma membrane reach into the grooves of the sheath, allowing for possible exchange of materials between the cells. (wikipedia.org)
Focal attachments have 10-15 nm distance between the cell membrane and the material surface and they are tight connections. (omicsonline.org)

Anatomy12

Organisms1

Diseases1

Chemicals and Drugs7

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes1

Disciplines and Occupations1

Neurons42

Astrocytes13

Differentiation3

Fibrillary Acidic Protein1

Oligodendrocytes5

Neuron9

Satellite glial3

Proliferation6

Mice6

Oligodendrocyte1

Microglia7

Acidic1

Astrocyte cultures2

Predominantly3

Protein7

Phagocytic2

Neural progenitor2

Nerve3

19961

Brain15

Glia2

MGCS1

Axons3

Primary Cultures1

Fibroblasts1

Cortical2

Lineages3

Cytoplasm1

Stem cells8

Fetal2

Rats1

Inflammation1

Uptake2

Phenotype2

Membranes1

Significantly3

Interactions4

Molecular1

Neuronal cell death1

Roles3

GFAP1

Schwann3

Biology2

Markers2

Mechanism2

Sympathetic1

Abstract1

Immune1

Ganglion3

Infection3

Cellular4

Membrane3