Germination of spores of bacillus subtilis. Medical search. Frequent questions

CEASE is a contact biological fungicide containing a patented strain of the bacterium Bacillus subtilis which works in two ways. (arbico-organics.com)
then active compounds called lipopeptides produced by each bacterium disrupt the germination and growth of invading pathogens. (arbico-organics.com)
A biological fungicide containing a strain of the bacterium Bacillus subtilis. (theprofarmer.com)
No other reports were reported for this bacterium spores in high-acid foods. (hindawi.com)
Bacillus subtilis, also known as the hay bacillus or grass bacillus is a Gram- positive bacterium, which is mostly found in soil. (markedbyteachers.com)
Next the peptidoglycan cortex forms between the two layers and the bacterium adds a spore coat to the outside of the forespore. (bionity.com)
B. subtilis is a mesophilic aerobic or facultative anaerobic gram-positive rod-shaped bacterium (Sonenshein and Losick 1994 ). (springeropen.com)

Eight B. subtilis strains PY79 (wild type) and 07 different div mutants were the subject of this study. (scialert.net)
All div mutant strains produce germination defective spores. (scialert.net)
PI3K inhibitor strains, nor in four other spore-formers of Peptococcaceae listed in IMG. (peptide-solubility.com)
However, a homolog for spoIIR was identified in all six strains, the product of which could interact with SpoIIGA for the processing of pro-σE into active σE, a sigma factor responsible for the expression of ~250 genes in the mother cell of Bacillus subtilis [68]. (peptide-solubility.com)
In this study, we constructed a series of genome-reduced strains by deleting non-essential regions in the chromosome of B. subtilis 168. (biomedcentral.com)
2015 ) showed that the cyclolipopeptides secreted by the B. subtilis strain ABS-S14 elicit a broad-spectrum antibiotic effect, reducing the number of Penicillium strains exposed to B. subtilis and cell-free cultivation by 96.2% and 90.9%, respectively. (springeropen.com)
Bacillus subtilis According to the ATCC database, all Bacillus subtilis strains have a biosafety level of 1. (probiotica.ru)

Oxidative activation of Bacillus cereus spores. (microbiologyresearch.org)

Under favorable environmental conditions, the spore breaks its dormancy and resumes growth in a process called spore germination and outgrowth. (tno.nl)
The transition of dormant bacterial spores to fully active vegetative forms can be divided into three distinct phases : activation, germination, outgrowth. (1library.net)

We therefore asked the question which bacterial spore formers are found in a typical. (tno.nl)

Sequencing and functional analysis of the genome of Bacillus subtilis strain 168. (microbiologyresearch.org)
Genome sequence and spore germination-associated transcriptome analysis will contribute to the understanding of the molecular mechanism of pathogenicity and spore germination of C. cassiicola . (biomedcentral.com)
Five such operons were identified in the genome of D. hafniense DCB-2 (Figure 8) including an octacistronic operon (Dhaf_0057-64) which encodes additional genes for Orn/Lys/Arg decarboxylase, DNA polymerase III δ' subunit, polymerase suppressor protein, and corrin/porphyrin methyltransferase, suggesting that the operon is used not only for the synthesis of a germinant receptor but for other metabolic activities in relation to sporulation/germination. (peptide-solubility.com)
The genome of B. subtilis harbors genes that likely encode MS channels of large (MscL) and small (MscS [YkuT, YhdY, and YfkC]) conductance, the main MS channels. (uconn.edu)
It has been reported that the genome-reduced Bacillus subtilis strain MBG874 showed clear advantages for the production of several heterologous enzymes including alkaline cellulase and protease. (biomedcentral.com)
Genome-scale genetic manipulation has a variety of effects on the physiological characteristics and cell metabolism of B. subtilis . (biomedcentral.com)

Spore-forming bacteria can be a problem in the food industry, especially in the canning industry. (tno.nl)
The bacteria of genus Clostridium are rod-shaped, gram-positive and spore-forming anaerobes. (biomedcentral.com)
The objective of this study was to evaluate the log reductions and thermal inactivation kinetics of spores of Bacillus subtilis bacteria and ascospores of Talaromyces flavus and Eupenicillium javanicum molds under influence of pH and sodium benzoate preservative. (hindawi.com)
Contamination and isolation of molds such as Talaromyces flavus and Eupenicillium javanicum and bacteria such as Bacillus subtilis in juices have been reported in past literatures [ 6 - 10 ]. (hindawi.com)
Two bacteria (GA1-23 and GA4-4) selected out of 943 microbial isolates because of their strong antibiotic activity against B. cactivora were identified as Bacillus subtilis and B. amyloliquefaciens , respectively, by the cultural characteristics, Biolog program and 16S rRNA sequencing analyses. (ppjonline.org)
Light and electron microscopy of the pathogen treated with the bacterial isolates showed the inhibition of spore germination with initial malformation of germ tubes and later formation of circle-like vesicles with no hyphal growth and hyphal disruption sometimes accompanied by hyphal swellings and shrinkages adjacent to the bacteria, suggesting their antibiotic mode of antagonistic activity. (ppjonline.org)
Experiment to investigate the effect of different antibiotics on the bacteria Bacillus subtilis: Aim: To see which antibiotic (Chloramphenicol, Erythromycin, Fusidic Acid, Oxacillin, Novobiocin, Penicillin, Streptomyan and Tetracycline) reacting with the Bacillus subtilis has the largest zone of inhibition in mm (+/- 0.5mm) and so which has the biggest effect on the bacteria. (markedbyteachers.com)
Gram-positive bacteria include many well -known genera like Streptococcus and Bacillus. (markedbyteachers.com)
In contrast to eukaryotic spores , which are produced by many eukaryotes for reproductive purposes, bacteria will produce a single endospore internally. (bionity.com)
Bacteria having a centrally placed endospore include Bacillus cereus , and those having a subterminal endospore include Bacillus subtilis . (bionity.com)
This unique all-spore formula effectively RECONDITIONS the gut by promoting microbial diversity and maintaining key health-promoting, commensal gut bacteria. (nourishmeorganics.com.au)
These bacteria are called spore-formers. (lisascounterculture.com)
When favorable condition prevails, (i.e. availability of water, appropriate nutrients) spores germinate which then forms vegetative cells of bacteria. (lisascounterculture.com)

It helps prevent the germination of fungal spores. (theprofarmer.com)

The activation of spores of Clostridium bifermentans by various treatments was investigated. (1library.net)
Both genes are also present in Clostridium spore-formers. (peptide-solubility.com)
The proposed genes are based on known developmental and genetic processes of sporulation and germination in Bacillus and Clostridium species. (peptide-solubility.com)
SUMMARY: The effect of hydrogen peroxide on the germination, colony formation and structure of spores of Clostridium bifermentans was examined. (microbiologyresearch.org)
Germination of spores of Clostridium bifermentans by certain amino acids, lactate and pyruvate in the presence of sodium or potassium ions. (microbiologyresearch.org)

3) Inhibition of mold: rosmarinic acid extracted from plants can inhibit phytophthora and its spores, which cause soil borne diseases, and effectively reduce the germination of zoospores. (zhenyibio.com)
such as Bacillus subtilis , is also one of the predominant microbes from bacterial genera found in soil and may also be introduced into the manufacturing process through poorly washed fruits. (hindawi.com)
Bacilli are natural members of the soil community and form spores for improved resistance under harsh environmental conditions. (allianceroots.com)

Figure 8 Putative diagram of sporulation and germination events in D. hafniense DCB-2. (peptide-solubility.com)

These spores had lost all or part of their dipicolinic acid (DPA) depending on the severity of the heat treatment. (tno.nl)
The MS channel mutants formed normal spores which accumulated optimal levels of osmolytes such as dipicolinic acid, glutamate and arginine as determined by phase contrast microscopy and 1 H NMR spectroscopy. (uconn.edu)
Dipicolinic acid could be responsible for the heat resistance of the spore, and calcium may aid in resistance to heat and oxidizing agents. (bionity.com)
iv) In the spore core, high levels of pyridine-2,6-dicarboxylic acid (dipicolinic acid, or DPA) enhance spore survival to solar UV. (photobiology.com)
Resistance of bacterial spores may be mediated by dipicolinic acid, a calcium ion chelator found only in spores. (lisascounterculture.com)

The present study deal with the germination analysis of spores of different div mutants ( divI, divII, divIV and divV ) of Bacillus subtilis in ALA and AGFK systems and the relationship of these mutations with ger genes. (scialert.net)
Tandem genes encoding sigma-factors for consecutive steps of development in Bacillus subtilis. (microbiologyresearch.org)

Control efficacy of B. subtilis GA1-23 and B. amyloliquefaciens GA4-4 on the cactus stem rot were not as high as but comparable to that of fungicide difenoconazole when they were treated simultaneously at the time of pathogen inoculation. (ppjonline.org)

In the laboratory, bacterial spores are 1-2 orders of magnitude more resistant to 254-nm UV-C radiation than are vegetative cells, and UV irradiation of dormant Bacillus subtilis endospores results mainly in formation of the unique spore photoproduct (SP) 5-thyminyl-5,6-dihydrothymine. (photobiology.com)
Made from a unique spore forming Bacillus subtilis strain, Alterion ® has an innovative formulation that ensures a hassle-free usage as well as an optimal activation in the small intestine. (adisseo.com)

4 In the inactivation of spores, alkylation of nucleic acids may be more important than changes in protein constituents. (basicmedicalkey.com)

2) Fungus inhibition: the inhibitory activity of rosmarinic acid on the mycelial growth and spore germination of different plant pathogenic fungi. (zhenyibio.com)
Both bacterial isolates significantly inhibited the conidial germination and mycelial growth of the pathogen with no significant difference between the two, of which the inhibitory efficacies varied depending on the cultural conditions such as temperature, nutritional compositions and concentrations. (ppjonline.org)

Bacillus subtilis forms dormant spores upon nutrient depletion. (tno.nl)
Bacillus pumilus increases nutrient availability and plant growth by producing extracellular enzymes that free bound nutrients in growth substrate. (allianceroots.com)
The B. subtilis vegetative cells are affected by changes in external environmental factors, such as nutrient deficiencies, accumulation of metabolites, temperature changes, etc. (springeropen.com)

Bacillus amyloliquefaciens lives symbiotically in the plant root zone and promotes root growth and enhances P availability. (allianceroots.com)

Low mscL-lacZ activity was detected in dormant spores. (uconn.edu)
Once inside the large intestine, these dormant spores can change into their active, vegetative forms and begin colonizing in the gut. (nourishmeorganics.com.au)

Hydrogen peroxide removed protein from spores-apparently from the coat-and treatment with dithiothreitol, which also removes spore-coat protein, increased the lethal effect of hydrogen peroxide 500-fold, suggesting that spore-coat protein has a protective effect against hydrogen peroxide. (microbiologyresearch.org)
The aim of this study was to establish whether this protein is involved in the D -alanylation process in Bacillus thuringiensis . (frontiersin.org)

Methods for the growth and enumeration of anaerobic spore-formers from cheese, with observations on the effect of nisin. (microbiologyresearch.org)

Peptidoglycan components (hexoamines, techoic acid, diaminopimelic acid) of germination defective spores exhibit variation from wild type, hence changes in wall composition leads to defects in cell division and then spore germination. (scialert.net)
pH value led to a decrease in the specific requirements for germination, an effect not produced by acid pH values. (1library.net)
For studies on the effect of mercaptoacetic acid on spores, the free acid was titrated to pH 7.4 with 10 N-NaOH, and added to spores suspended in phosphate buffer. (1library.net)
After activation by heating or exposure to acid or alkali, spores were washed with phosphate buffer and stored as suspensions in this buffer at 4' and at room temperature (16-20') in screw-capped bottles three-quarters full, tightly screwed down to minimize aeration. (1library.net)
Rosmarinic acid has obvious inhibitory effect on Bacillus subtilis, Micrococcus luteus, Escherichia coli, Staphylococcus aureus and Ralstonia solani. (zhenyibio.com)
1) Bacterial inhibition: Rosmarinic acid has significant inhibitory effect on Bacillus subtilis, Pseudococcus sanguis and Escherichia coli. (zhenyibio.com)
As part of a sensory mechanism capable of initiating germination, CFLE degrades a spore-specific peptidoglycan constituent called muramic-acid delta-lactam that comprises the outer cortex. (unl.edu)
Pre-incubation of spores with Cu 2+ before treatment with hydrogen peroxide produced a similar increase, but this could be eliminated by washing the spores with dilute acid or ethylenediamine tetraacetate. (microbiologyresearch.org)

UV resistance of spores and vegetative cells of B. subtilis DNA repair mutants. (photobiology.com)

Germination of spores occurs in response Celecoxib to nutrients (or germinants) which are often single amino acids, sugars or purine nucleosides, and is initiated by binding of germinants to receptors located in the spore's inner membrane [69, 70]. (peptide-solubility.com)
The mutant spores germinated normally in presence of various germinants including L-alanine, dodecylamine, and high pressure and also exhibited normal wet heat resistance. (uconn.edu)

To elucidate the physiological processes that occur during the transition of the dormant spore to an actively growing vegetative cell, we. (tno.nl)

Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in bacterial endospore germination. (unl.edu)

SleB translocates across the forespore inner membrane and is deposited as a mature enzyme in the cortex layer of the spore. (unl.edu)
The cortex lies beneath the spore coat and consists of peptidoglycan. (bionity.com)

Bacillus subtilis colonize the developing root system, competing with disease organisms that attack root It inhibits plant pathogen spore germination, disrupts germ tube growth, and interferes with the attachment of the pathogen to the plant. (bighaat.com)
Ascospore germination and growth in fruit products after pasteurization reaching commonly 10 5 -10 6 /g or mL in number are a concern since it can cause food spoilage and in some cases foodborne diseases. (hindawi.com)
Overall, this study shows that MS channels in B. subtilis play a critical role in regulation of cell's internal turgor pressure, but are not essential for growth, sporulation or spore germination. (uconn.edu)
In this study, we investigated the effects of volatile organic compounds (VOCs) produced by Bacillus subtilis CF-3 on the growth and development of Colletotrichum gloeosporioides and evaluated the elicitation of active defense responses in harvested litchi fruits. (springeropen.com)
Nitrogen-fixing microorganisms provide nitrogen for crop growth, and enzymes aid plant growth, seed germination, and fruiting. (pharmacyfreak.com)
When the free endospores are placed in an environment that supports growth, the endospores will revert back to a vegetative cell in a process called germination. (lisascounterculture.com)

The DNA is replicated and a membrane wall known as a spore septum begins to form between it and the rest of the cell. (bionity.com)

The present paper describes experiments on the effect of reducing agents and pH value as activating agents for spores of this organism and also investigations of the inorganic ion require- ment for germination. (1library.net)

These results facilitate the exploration of the molecular pathogenic mechanism of C. cassiicola in cucumbers and the understanding of molecular and cellular processes during spore germination. (biomedcentral.com)

The region between yvsA (293°) and yvqA (289°) of the Bacillus subtilis chromosome has been sequenced within the framework of the B. subtilis 168 international sequencing programme. (microbiologyresearch.org)

In Bacillus subtilis, these receptors are encoded by the homologous tricistronic gerA, gerB and gerK operons [70]. (peptide-solubility.com)

Fluorescent Pseudomonas and Bacillus spp. (ppjonline.org)
در این پژوهش، پتانسیل بیوکنترلی ۱۲ سویه باکتری پروبیوتیک از جنس‌های Alcaligenes ، Arthrobacter ، Bacillus ، Delftia ، Lysinibacillus ، Pseudomonas و Stenotrophomonas علیه A. rabiei بررسی گردید. (ac.ir)

Spore formation (sporulation) occurs when nutrients, such as sources of carbon and nitrogen are depleted. (lisascounterculture.com)
After host infection by C. difﬁcile spores, germination occurs, and the vegetative forms multiply in the gut. (studyres.com)

Previous studies on biocontrol agents include the effects of foliar and root applications of Lysobacter enzymogenes strain C3 in the suppression of conidial germination and leaf spot development after pathogen inoculation ( Kilic-Ekici and Yuen, 2004 ). (ppjonline.org)
Bacillus subtilis is not a human pathogen and it can contaminate food but rarely causes food poisoning. (markedbyteachers.com)

Activation of spores or the breaking of dormancy is commonly achieved by heating spores in aqueous suspension, a process first described by Evans & Curran (1943). (1library.net)

Because Alliance Biologics is manufactured as long-lived resistant spores form containing a spore germination inhibitor, our product has the longest shelf life on the market (at least 2 years). (allianceroots.com)

In a previous publication (Gibbs, 1964), some of the factors which affect the germina- tion of heat-activated spores of C. bifermentans were described. (1library.net)

Please use this product under the supervision of a trained practitioner MegaSporeBiotic™ is a 100% spore-based, broad-spectrum probiotic clinically shown to maintain healthy gut barrier function. (nourishmeorganics.com.au)
MegaSporeBiotic™ is a 100% spore-based, broad-spectrum probiotic clinically shown to maintain healthy gut barrier function. (nourishmeorganics.com.au)
MegaSporeBiotic is a probiotic blend of 5 Bacillus spores that have been clinically shown to maintain healthy gut barrier function and overall immunity. (nourishmeorganics.com.au)
Oral spore-based probiotic supplementation was associated with a reduced incidence of post-prandial dietary endotoxin, triglycerides, and disease risk biomarkers. (nourishmeorganics.com.au)
What, you haven't heard about probiotic spores? (lisascounterculture.com)
I first learned about probiotic bacterial spores earlier this year when I was considering recommending a probiotic supplement. (lisascounterculture.com)

In addition to enzymes, B. subtilis is also used for the production of chemicals. (biomedcentral.com)
The spore coat is impermeable to many toxic molecules and may also contain enzymes that are involved in germination. (bionity.com)

Spores present in ingredients or present in the processing environment severely challenge the preservation process since their thermal resistance may be very high. (tno.nl)

Bacterial spores are highly resistant, dormant structures (i.e. (lisascounterculture.com)

requirement of heat-activated spores for sodium ions for germination was also found. (1library.net)
To conclude, the combination of low pH and sodium benzoate provided the best method for spore inactivation, which could enhance food safety and extend food's shelf life. (hindawi.com)

iii) The spore coat layers, particularly the inner coat, plays a protective role in the solar UV resistance of spores. (photobiology.com)

Apart from the antibiotic effects, GS increases the rate of evaporation from the plant surface, reducing periods of surface wetness and thereby indirectly inhibiting spore germination. (elsevierpure.com)

Alterations in metal content of spores of Bacillus megaterium and the effect on some spore properties. (microbiologyresearch.org)
Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var. (nourishmeorganics.com.au)

Bacterial spores are highly resistance to heat, dehydration, radiation and chemicals. (lisascounterculture.com)

Spores of Bacillus subtilis were subjected to relatively mild heat treatments in distilled water and properties of these spores were studied. (tno.nl)
Even after relatively mild heat treatments these spore lost already a small but. (tno.nl)
Heat-activated spore suspensions became partially de-activated on storage unless they were continuously aerated. (1library.net)
9 Revival in formaldehyde-treated Bacillus subtilis spores was increased by up to 1 log using heat shock for 30 minutes before plating out. (basicmedicalkey.com)
It was postulated by Williams and Russell 10 that the heat shock could enhance and/or trigger germination after contact with formaldehyde. (basicmedicalkey.com)

Anatomy12

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Phenomena and Processes52

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Heat5