• Mutations in keratin genes have been documented in many disorders of the skin, nails, hair, and mucous membranes. (nih.gov)
  • Mutations in this gene have been associated with Johanson-Blizzard syndrome. (nih.gov)
  • Mutational analysis revealed point-mutations in the SOD1 gene to cosegregate with the disease in these pedigrees. (bmj.com)
  • The 11 genes with a high frequency of mutation differed between cancers, and mutations in many DNA DSB repair E3 ligase genes were related to a higher total mutation burden. (bmbreports.org)
  • Mutations in the AFF2 gene cause fragile XE syndrome, a condition characterized by mild intellectual disability and learning difficulties. (medlineplus.gov)
  • mutations high frequency of IDH1/2 mutations in oligodendrogliomas, astrocytomas and in alteRations in the RB1 pathway in The TET2 gene encodes the -KG- secondary glioblastomas derived thereof low-gRade diffuse gliomas lacking dependent enzyme that catalyses suggests that these tumours share a common genetic alteRations the conversion of 5-methylcytosine to common progenitor cell population. (who.int)
  • Some mutations in the S gene may lead to changes in the spike protein which result in inhibition of contact and entry of the virus into human cells, however in the case of the VOC, they contain mutations in the S gene that enhance the process of contact and entry into human cells, increasing transmissibility of the virus. (who.int)
  • Currently, manufacturers are focusing on and targeting assays to mutations in the S gene. (who.int)
  • The betA gene sequence reported in this study contains several base substitutions with that of reported sequences in GenBank, resulting in the altered amino acid sequences of the translated proteins. (scialert.net)
  • DNA sequence analysis of the 3′-end of the Coagulase ( coa ) gene revealed heterogeneity in the 81 bp tandem repeats region that encoding repeated 27-amino-acid sequences in the C-terminal region. (hindawi.com)
  • The MasterMix also contains Epicentre's PCR Enhancer, which substantially improves yield, efficiency, and specificity of amplification of many target sequences, especially those with a high GC content or secondary structure. (genengnews.com)
  • The 20 kb lambda amplification was completed in just under three hours, while the 30 kb fosmid sequences were amplified in 5.5 hours. (genengnews.com)
  • MicroRNAs (miRNAs) negatively regulate the expression of the target genes by binding to ʻseed sequences' in the 3'‑untranslated region (3'‑UTR) mRNA transcripts, and the variants within or nearby ʻseed sequences' may compromise or enhance miRNA/mRNA interaction leading to either ʻloss‑of‑function' or ʻgain‑of‑function' effects. (spandidos-publications.com)
  • miRNAs bind to complementary sequences present in the 3′-untranslated regions (3′-UTR) of target gene mRNAs and, thus, regulate translational interference or degradation of mRNAs ( 8 ). (spandidos-publications.com)
  • To resolve phylogenetic relationships among ries of bacterial clonal complexes by using concatenated isolates, we sequenced 68 isolates from Europe and North sequences of housekeeping genes when within-loci and America at 1 chromosomal locus (16S-23S ribosomal RNA between-loci recombinations are infrequent ( 5 ). (cdc.gov)
  • Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)
  • Standard chromosome analysis by G-banding has a limited resolution, but molecular cytogenetic techniques, such as multi-subtelomeric FISH, microdeletion FISH, multicolour FISH and comparative genomic hybridisation (CGH), have played an important role for the diagnosis of MR during the past decade. (bmj.com)
  • Comparative genomic hybridisation (CGH) was used to screen 76 vestibular schwannomas from 76 patients (66 sporadic and 10 NF2 related) to identify other chromosome regions that may harbour genes involved in the tumorigenesis. (bmj.com)
  • SGTP), which alongside genomic sequencing, has facilitated sample of ICATT specimens, were assessed by SGTF/SGTP early monitoring of emerging variants. (cdc.gov)
  • Stargardt disease (STGD1) is a form of inherited retinal dystrophy attributed to variants affecting function of the large ABCA4 gene and is arguably the most complex monogenic disease. (nature.com)
  • In order to meet the grand challenge of human genetics, that is, to understand what causes disease and translate this knowledge to improve health outcomes, we need to know the number and population frequency of disease variants, the magnitude of their effects on phenotype and gene-gene and gene-environment interactions. (nature.com)
  • For monogenic disorders, the genetic architecture is simplified as disease variants are, by definition, highly penetrant and environmental and gene-gene interactions are minimized. (nature.com)
  • Patients with ABCA4 -related disease have pathogenic variants in the ABCA4 gene which encodes a large, 2273 amino acid photoreceptor-specific transporter involved in the removal of toxic retinoid compounds from photoreceptors [ 20 ]. (nature.com)
  • The role of HMT gene variants in prostate cancer remains unknown. (cancerindex.org)
  • The results have demonstrated that many new variants of the coa gene are present in Minia, Egypt, different from those reported in the previous studies. (hindawi.com)
  • Variants in the UBR1 gene are not associated with chronic pancreatitis in Japan. (nih.gov)
  • In this pilot study, we sought to identify clinical and molecular factors that distinguish HGSOC patients who share similar clinical characteristics and pathology at diagnosis with exceptional survival outcomes, either LT or ST, through integrated analysis of clinical features, germline variants, somatic genomic alterations, and tumor immune microenvironment. (biomedcentral.com)
  • Luciferase assays and online miRNA databases were used to validate that tumor protein p53 (TP53) is the target gene of miR‑125b. (spandidos-publications.com)
  • Gene dose alterations can cause mental retardation (MR), congenital malformations and miscarriages. (bmj.com)
  • Screening for chromosomal imbalances by array CGH, whether using cDNA 23 or BAC clones, 24 has mainly been performed on cancer samples, 25- 30 which usually contain large gene dose alterations. (bmj.com)
  • Although array CGH has provided a higher resolution compared to conventional CGH, it has not yet become a widely applied method for the analysis of gene dose alterations in individuals with idiopathic mental retardation. (bmj.com)
  • Problems that arise during DNA replication can drive genomic alterations that are instrumental in the development of cancers and many human genetic disorders. (elifesciences.org)
  • Defects in DNA double-strand break (DSB) repair signaling permit cancer cells to accumulate genomic alterations that confer their aggressive phenotype. (bmbreports.org)
  • DNA DSB repair E3 ligase genes are involved in tumor suppressive or oncogenic functions, such as RNF168 and FBXW7, by assisting the functionality of these genomic alterations. (bmbreports.org)
  • This case study reports a rare double minute chromosome via FLT3 amplification in CMML by using array-based comparative genomic hybridization and fluorescence in situ hybridization analyses. (hindawi.com)
  • The NF2 gene is a tumour suppressor on chromosome 22. (bmj.com)
  • In 1986, the NF2 gene was mapped to chromosome 22, and in 1993, two groups independently isolated the gene and named its encoding protein merlin or schwannomin. (bmj.com)
  • Linkage analysis in 18 familial ALS pedigrees associated the gene encoding Cu/Zn superoxide dismutase ( SOD1 ) on chromosome 21 to the syndrome. (bmj.com)
  • Validation of whole genome amplification for analysis of the p53 tumor suppressor gene in limited amounts of tumor samples. (diva-portal.org)
  • Differential PCR was performed to study gene amplification and expression of several proto-oncogenes (K-ras, c-jun, c-fos, c-myc, c-sis and erb-B2) and tumor suppressor genes (p53 and p16). (cdc.gov)
  • None of the genes studied were amplified in tumor cells. (cdc.gov)
  • Gene ID: 5925, OMIM 614041 ) gene, which encodes a tumor suppressor protein. (molvis.org)
  • This study provides a broad list of candidate genes as potential biomarkers for genomic instability and novel therapeutic targets in cancer. (bmbreports.org)
  • The window of opportunity to tailor therapeutic interventions to control progressive disease is limited due to the inherent cellular heterogeneity and genomic instability of HGSOC. (biomedcentral.com)
  • Dr Kasuya Motomura amplification bias, we assessed genome secondary events reflecting progressive wide chromosomal imbalance by array genomic instability, a hallmark of CGH (Agilant 105K) in DNA from 2-5 glioblastomas. (who.int)
  • Geographic representativeness and or failed S -gene target amplification in certain multitarget median interval from specimen collection to result were cal- reverse transcription-polymerase chain reaction (RT-PCR) culated for both data sources. (cdc.gov)
  • With regard to amplification, polymerase chain reaction (PCR) is the most popular method. (frontiersin.org)
  • Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of β-lactamase encoding genes (bla) including bla NDM-1 and plasmid-mediated quinolone and aminoglycoside resistance determinants. (who.int)
  • Genomic amplification of FLT3 has been reported in solid tumors including colorectal cancer, breast cancer, and gastric cancer [ 9 ]. (hindawi.com)
  • Methods: This case-control study conducted in Srinagar, Kashmir (North India) between 2013 and 2015 was designed to evaluate risk induced due to MTH-FR 677C>T gene polymorphisms to contribute in susceptibility for migraine in Kashmir population (North India). (scirp.org)
  • Results Our results suggest that, in the Thai population, polymorphisms in XRCC1 and OGG1 genes, particularly in combination, are associated with increased susceptibility to CCA, and that their role as modifiers of the effect of smoking and alcohol consumption influences the risk of CCA. (bmj.com)
  • Genomic epidemiology and antibiotic susceptibility profiling of uropathogenic Escherichia coli among children in the United States. (cdc.gov)
  • This gene encodes a member of the trithorax group of transcriptional activators. (cancerindex.org)
  • The S gene is one of the structural genes of the virus which encodes for a protein that sits on the surface of the SARS CoV-2 virus. (who.int)
  • This study implied that KCNK2, KCNK4, KCNK5 and KCNK15 are potential targets of precision therapy for patients with thyroid cancer and these genes are new biomarkers for the therapeutic target for thyroid cancer. (jcancer.org)
  • Chromothripsis is a mutational phenomenon characterized by massive, clustered genomic rearrangements that occurs in cancer and other diseases. (lu.se)
  • To download a certificate of analysis for Genomic DNA from Gluconacetobacter diazotrophicus strain PA1 5 ( 49037D-5 ), enter the lot number exactly as it appears on your product label or packing slip. (atcc.org)
  • The certificate of analysis for that lot of Genomic DNA from Gluconacetobacter diazotrophicus strain PA1 5 ( 49037D-5 ) is not currently available online. (atcc.org)
  • These samples are frequently only available in limited quantities hampering genomic analysis. (diva-portal.org)
  • Overall this data indicate a limited random loss of genomic regions supporting the use of whole genome amplification for genomic analysis. (diva-portal.org)
  • characterization by metaphase analysis, comparative genomic hybridization, and semi-quantitative PCR. (atlasgeneticsoncology.org)
  • We performed a molecular cytogenetic investigation of the scleractinian coral Acropora solitaryensis, which is dominant in the temperate region of Japan (30-35°N). Molecular cytogenetic analysis, using fluorescence in situ hybridization (FISH), was carried out for karyotyping and gene mapping. (bioone.org)
  • Proteins/genes with a putative catabolic role and bacterium adaptation mechanisms during OPP degradation were identified via genomic and proteomic analysis. (nature.com)
  • Transcription analysis of all putative catabolic genes established their role in the metabolism of OPP. (nature.com)
  • Genomic analysis identified two orthologous operons encoding the ortho -cleavage of benzoic acid (BA) ( ben/cat ). (nature.com)
  • Expanding the mutational spectrum in Johanson-Blizzard syndrome: identification of whole exon deletions and duplications in the UBR1 gene by multiplex ligation-dependent probe amplification analysis. (nih.gov)
  • Random Amplified Polymorphic DNA (RAPD) analysis was carried out to study the genomic changes using 10 different random primers. (cdc.gov)
  • No significant genomic changes were observed by RAPD analysis. (cdc.gov)
  • Agarose gel analysis of the PCR product showed highly specific amplification even with 1 pg of template (Figure 1). (genengnews.com)
  • Polymorphisms in BER genes were analysed using a PCR with high-resolution melting analysis. (bmj.com)
  • The T-RFLP analysis based on 16S rRNA gene showed that inoculation with RMB7 or its supernatant have a major impact on the indigenous rhizosphere bacterial population. (biomedcentral.com)
  • The Kyoto encyclopedia of genes and genomes pathway analysis revealed that protein digestion and absorption, amino acyl-trNA biosynthesis, unsaturated fatty acid biosynthesis, nucleotide metabolism and carbon metabolism were the main enrichment pathways in aging. (bvsalud.org)
  • Genomic epidemiological analysis of county-scale Yersinia pestis spread pattern over 50 years in a Southwest Chinese prefecture. (cdc.gov)
  • Phylogenetic analysis with DNA amplification technologies. (who.int)
  • Genomic DNA is extracted from plant leaves and subjected for treatment to remove contaminant RNA, protein and polysaccharide, Genomic DNA is measured by Nanodrop (a spectrophotometer technology, not by fluorometer/Qubit) and provided in 1.5-ml tubes at a concentration of 0.5mg/ml or 1mg/ml in TE buffer. (delos.info)
  • RARA was not checked in any of the cases, although a M3 presenting with a breakpoint in 17q21 is very likely to bear a RARA fusion gene and protein. (atlasgeneticsoncology.org)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • High density arrays of extraordinarily sensitive integrated microring resonators will allow many gene and protein signatures to be simultaneously quantitated from a single patient sample. (nih.gov)
  • The recognition component of this pathway, encoded by this gene, binds to a destabilizing N-terminal residue of a substrate protein and participates in the formation of a substrate-linked multiubiquitin chain. (nih.gov)
  • The AFF2 gene provides instructions for making a protein that is found in the nucleus of cells but whose function is not well understood. (medlineplus.gov)
  • As a result, the AFF2 gene is turned off (silenced), and no protein is produced from it. (medlineplus.gov)
  • Rarely, small deletions of genetic material from the AFF2 gene are associated with fragile XE syndrome, although how these deletions affect the protein and lead to intellectual disability is unknown. (medlineplus.gov)
  • The A and B clones of Borrelia burgdorferi sensu stric- maintenance of these clonal complexes in natural bacterial to, distinguished by outer surface protein C ( ospC ) gene populations ( 3 , 4 ). (cdc.gov)
  • The viral S gene is important as it codes for the Spike protein which is the molecule that makes contact with, and allows entry of the virus into susceptible host cells, causing infection. (who.int)
  • GENE-UP® BREWPRO™ BACTERIA PLUS provides genomic-level detection of the lactic-acid bacteria species paired with risk-based detection by targeting the horA/horC hop resistance genes in the same assay. (biomerieux.com)
  • Real-time RT-PCR cycle threshold (Ct) results average number of SGTF/SGTP and sequencing results were for three gene targets ( S , N , and ORF1ab ) were reported to U.S. (cdc.gov)
  • CDC also collects genomic sequencing ing results classified by SGTF and SGTP (Figure). (cdc.gov)
  • Interestingly, unlike other isothermal methods, such as LAMP or helicase-dependent amplification (HDA), which can only amplify very short segments of DNA, the QPA method can amplify hundreds of basepairs, generating "long DNA molecules for downstream applications, like sequencing," Kankia said. (genomeweb.com)
  • The authors performed PCR amplification of all five coding exons of SOD1 followed by direct DNA sequencing using forward and reverse primers. (bmj.com)
  • Based on 16S rRNA gene sequencing, strain RMB7 was identified as Bacillus specie. (biomedcentral.com)
  • We reviewed published microarray and RNA sequencing studies in which gene expression profiles were compared between human RB and normal retina tissues. (molvis.org)
  • FISH mapping of the rRNA gene was carried out with a probe generated by PCR amplification using rRNA gene primers. (bioone.org)
  • By extraction an accurate DNA and using suitable primers, H6H gene can be isolated completely. (ac.ir)
  • NEW YORK (GenomeWeb) - In an effort to design primers that might solve the conundrum of the plateau effect in PCR amplification, researchers at Ohio State University and Ilia State University in the country of Georgia have created a method called quadruplex priming amplification. (genomeweb.com)
  • By comparison, loop-mediated isothermal amplification (LAMP) requires six primers, Kankia said. (genomeweb.com)
  • Each reaction included 1X MasterMix, 12.5 pmol each of forward and reverse primers, and 50 ng of human genomic DNA. (genengnews.com)
  • How Reliable Are Gene Expression-Based and Immunohistochemical Biomarkers Assessed on a Core-Needle Biopsy? (lu.se)
  • Combining key elements of our VERIFLOW® DNA signature capturing technology with our powerful multiplex RT-PCR plateform, GENE-UP® BREW offers a superior identification of known beer spoilers. (biomerieux.com)
  • To evaluate the TAQXpedite System for multiplex amplification, nine regions of the human Duchenne muscular dystrophy (DMD) gene were amplified. (genengnews.com)
  • The nine-band multiplex PCR of the human DMD gene was successfully completed in 52 minutes with the TAQXpedite Universal MasterMix (Figure 3). (genengnews.com)
  • Southern blot hybridization and PCR amplification confirmed the presence of the hygromycin phosphotransferase gene in genomic DNA. (gc.ca)
  • In this study, a rapid DNA extraction method (RDEM), recombinase aided amplification (RAA), and chemical heating packet (CHP) are integrated and termed as RRC platform for on-site detection of nucleic acid. (frontiersin.org)
  • These two platforms have the potential to use either isothermal nicking enzyme amplification reaction (NEAR) or recombinase polymerase amplification (RPA). (genomeweb.com)
  • 5, 6 The NF2 gene is a tumour suppressor, as corroborated by studies showing complete inactivation of the gene in more than 60% of schwannomas, either by mutation in both alleles or by loss of one allele and mutation in the other. (bmj.com)
  • Chromothripsis contributes to oncogene amplification and to inactivation of genes such as mismatch-repair-related genes. (lu.se)
  • Stettner GM, Shoukier M, Hoger C, Brockmann K, Auber B. Familial intellectual disability and autistic behavior caused by a small FMR2 gene deletion. (medlineplus.gov)
  • The keratin 15 gene comprises 8 exons spanning approximately 5.1 kb on 17q21, and the keratin 19 gene consists of 6 exons covering approximately 4.7 kb on 17q21. (nih.gov)
  • The nucleic acid-based method generally comprises three steps including nucleic acid extraction, amplification, as well as product detection. (frontiersin.org)
  • A high quantity of DNA is extracted from cell suspension using the magnetiQ Blood & Cell DNA Extraction Kit, resulting in a lower CT value for GAPDH gene. (galenvs.com)
  • Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
  • The expression of the B-glucuronidase gene in the needles of regenerated seedlings support the potential for long-term transgene expression in spruce. (gc.ca)
  • Of interest, we have observed in a rodent transgenerational model of intrauterine growth restriction (IUGR) that a diet supplemented with essential nutrients, yet unaltered in its caloric content, prevents adult metabolic disease and is associated with abrogation of reprogrammed gene expression. (nih.gov)
  • We hypothesized that a high fat diet in non-human primates would induce changes in hepatic chromatin structure resulting in altered expression of fetal genes critical to the development of childhood and adult obesity. (nih.gov)
  • The novel innovation and significance resides within its potential to provide (1) an expanded understanding of the mechanism through which a maternal high fat diet reprograms primate gene expression and (2) a simple intervention (essential nutrient supplementation with neither diet nor behavioral modification) with tremendous potential impact given the current obesity epidemic and the lack of efficacious therapeutics. (nih.gov)
  • These results indicate that MWF is capable of inducing cellular changes and changes in gene expression of certain oncogenes in BALB/c-3T3 cells and that these cells possess neoplastic potential. (cdc.gov)
  • MicroRNAs (miRNAs), a class of endogenous small non-coding RNAs of 20-25 nucleotides, modulate the expression of genes at the post-transcriptional level ( 7 ). (spandidos-publications.com)
  • Mutation and expression frequencies of these genes were analyzed in COSMIC and TCGA. (bmbreports.org)
  • A comprehensive understanding of gene expression in human RB is essential for the development of safe and effective new therapies. (molvis.org)
  • We investigated the expression of genes of interest using quantitative reverse transcription PCR. (molvis.org)
  • The regulatory elements required for this cell-specific gene expression are likely located within its proximal promoter. (molvis.org)
  • Text mining biomedical literature to identify extremely unbalanced data for digital epidemiology and systematic reviews: dataset and methods for a SARS-CoV-2 genomic epidemiology study. (cdc.gov)
  • The current study aimed to use Coagulase gene polymorphism to identify methicillin-resistant Staphylococcus aureus (MRSA) subtypes isolated from nasal carriers in Minia governorate, Egypt, evaluate the efficiency of these methods in discriminating variable strains, and compare these subtypes with antibiotypes. (hindawi.com)
  • The aim of this study was to use coa gene polymorphism to identify MRSA subtypes isolated from nasal carriers in Minia governorate, Upper Egypt, evaluate the efficiency of these typing methods in discriminating variable strains, and compare these subtypes with antibiotypes. (hindawi.com)
  • To compare the TAQXpedite System with three other suppliers' fast PCR kits, a 270 bp sequence that is 80% GC-rich was amplified from the human FMR1 (Fragile X) gene. (genengnews.com)
  • To facilitate future mutation detection analyses, we have therefore characterized the intron-exon organization of the human keratin 15 and keratin 19 genes. (nih.gov)
  • The GTPase signalling molecules RhoA and Rac1 regulate merlin function, but to date only mutation in the NF2 gene has been identified as a causal event in schwannoma formation. (bmj.com)
  • Characteristics of ST survival included focal copy number gain of CCNE1 , lack of BRCA mutation signature, low homologous recombination deficiency scores, and the presence of ESR1-CCDC170 gene fusion. (biomedcentral.com)
  • Nearly all cases are caused by a mutation called a CCG trinucleotide repeat expansion, in which the CCG trinucleotide is abnormally repeated more than 200 times, which makes this region of the gene unstable. (medlineplus.gov)
  • Studies on the prevalence of Using a proteinase K and a saline extrac- the FVL mutation have revealed an uneven tion protocol, genomic DNA was extracted ethnic and geographical distribution. (who.int)
  • Conclusions: We conclude that MTHFR gene C677T polymorphism has no role in predisposition to the migraine in our population and cannot serve as a predictive factor for the risk of migraine. (scirp.org)
  • ABSTRACT This study aimed to evaluate the association of plasma MIF level and -173 G/C single nucleotide polymorphism of the MIF gene with the occurrence, severity and mortality of sepsis patients. (who.int)
  • High MIF levels and MIF -173G/C gene polymorphism are powerful predictors of the severity of sepsis and its outcome. (who.int)
  • In this study, choline dehydrogenase ( betA ) gene encoding for glycine betaine biosynthesis in Escherichia coli isolated from salted shark ( Scoliodon sp. (scialert.net)
  • Recently, we reported that functional wheat inositol penta kis phosphate kinase ( TaIPK1 ) is involved in PA biosynthesis, however, the functional roles of the IPK1 gene in wheat remains elusive. (frontiersin.org)
  • PCR-amplification further confirmed the presence of genes involved in the biosynthesis of these two bioactive lipopeptide compounds. (biomedcentral.com)
  • Zyagen offers a pure and intact high molecular size control genomic DNA of 32 different plant species. (delos.info)
  • Gecz J. The FMR2 gene, FRAXE and non-specific X-linked mental retardation: clinical and molecular aspects. (medlineplus.gov)
  • cDNA synthesis was performed with SuperScript III reverse transcription (Invitrogen, https://www.thermofisher.com/us/en/home/brands/invitrogen.html) using a single primer located within the 2C and the 3 UTR. (cdc.gov)
  • Some studies suggest that it acts as a transcription factor, which means that it attaches (binds) to specific regions of DNA and helps control the activity of other genes, although the identity of these genes is unknown. (medlineplus.gov)
  • We also demonstrated that the −657 to +411 DCT promoter fragment efficiently directs RB cell-specific transcription of the luciferase reporter gene in cell lines. (molvis.org)
  • Genomic DNA was purified from the peripheral blood samples using the Maxwell RSC Blood DNA kit (Promega) as per the manufacturer's recommendations. (hindawi.com)
  • PCR amplifications were set up according to the manufacturer's directions, including the reactivation of hot-start enzymes where applicable. (genengnews.com)
  • PGPR stimulate plant growth and productivity by improving nutrient supply, contributing in plant defense and protection through triggering various growth- and defense-related genes to induce cellular response, consequently improving the crop yield [ 2 ]. (biomedcentral.com)
  • Genomic DNA isolated from Gluconacetobacter diazotrophicus Strain PAI5. (atcc.org)
  • Therefore, an engineered strain, FJ-PYC, was constructed by overexpressing the pyc gene, which increased the PMA titer by 15.1% compared with that from the wild-type strain in a 5-L stirred-tank fermentor. (biomedcentral.com)
  • PCR amplification of this region showed DNA bands of different size and number that could be further differentiated by using restriction enzyme AluI [ 10 ]. (hindawi.com)
  • Based on our findings, we propose that IFSA is a potential threat to genomic stability in eukaryotes. (elifesciences.org)
  • We therefore examined whether polymorphisms in DNA base-excision repair (BER) genes, XRCC1 G399A and OGG1 C326G, were associated with CCA risk and whether they modified the effect of smoking and alcohol drinking in the Thai population. (bmj.com)
  • ABI 3500Dx automated DNA sequencer (Applied Biosystems, https://www.thermofisher.com/us/en/home/brands/applied-biosystems.html). (cdc.gov)
  • Several whole genome amplification (WGA) protocols have been developed with reported varying representation of genomic regions post amplification. (diva-portal.org)
  • YY1-mediated chromatin loops play substantial roles in basic biological processes like gene regulation, cell differentiation, and DNA replication. (researchgate.net)
  • Coagulase gene PCR-RFLPs exhibited 10 patterns that ranged from 1 to 8 fragments with AluI digestion. (hindawi.com)
  • The study also proposed another possible mechanism of FLT3 genes in leukemogenesis. (hindawi.com)
  • In this study, RNAi-mediated gene silencing was performed for IPK1 transcripts in hexaploid wheat. (frontiersin.org)
  • Finally, earlier this month, a study in Biopolymers revealed they have made the liner method exponential, with 10 10 -fold amplification in less than 40 minutes. (genomeweb.com)
  • however, limited data exist regarding the overall genomic prospect and functional result of these modifications. (bmbreports.org)
  • One region of the AFF2 gene contains a particular DNA segment known as a CCG trinucleotide repeat, so called because this segment of three DNA building blocks (nucleotides) is repeated multiple times within the gene. (medlineplus.gov)
  • GENE-UP® BREWPRO™ WILD YEAST PLUS delivers complete, accurate, and actionable results through the addition of proprietary and predictive gene targets. (biomerieux.com)
  • The identified isolates were tested by Coagulase gene RFLP typing. (hindawi.com)
  • Out of 58 MRSA isolates 15 coa types were classified, and the amplification products showed multiple bands (1, 2, 3, 4, 5, and 8 bands). (hindawi.com)