Genomes bacterial secretion systems. Medical search. Frequent questions

The genes encoding T6SS have been reported a few years ago as being present in different bacterial species, although it was not clear at that time whether those genes act together or are important in bacteria-host interactions [ 7 ]. (biomedcentral.com)
In addition to Vgr and Hcp proteins, the actual hallmark of this novel system is the presence of an AAA+ Clp-like ATPase and of two additional genes icmF and dotU , encoding homologs of T4SS stabilising proteins [ 10 ]. (biomedcentral.com)
While often reported that the T6SS was discovered in 2006 by researchers studying the causative agent of cholera, Vibrio cholerae, the first study demonstrating that T6SS genes encode a protein export apparatus was actually published in 2004, in a study of protein secretion by the fish pathogen Edwardsiella tarda. (wikipedia.org)
For this, it identifies and clusters co-localized genes encoding proteins of secretion systems using sequence similarity search with Hidden Markov Model (HMM) protein profiles. (bvsalud.org)
Genes belonging to proteases, bacterial exporters and DNA stabilization were found to be under strong positive selection, thus facilitating pathogenicity and survival of the outliers. (frontiersin.org)
Then, we selected 48 genes that may encode uncharacterised effectors (e. g. no detectable amino acid identity to other proteins or with a particular putative biochemical activity) from the genome of C. trachomatis strain L2/434/Bu. (europa.eu)
We show two examples using Salmonella genomes: finding within minutes the presence of genes in the SPI-2 pathogenicity island in a collection of 926 genomes and identifying single nucleotide polymorphisms associated with fluoroquinolone resistance in three genes among 190,209 genomes. (biomedcentral.com)
Even the seemingly simple task of identifying all bacterial strains within a collection that contains a specific antimicrobial resistance gene or other genes of interest is a computational challenge for the large data sets that are currently available. (biomedcentral.com)
This strategy originates with novel fusion-oncogenes identified from patient samples that require functional validation in vertebrate systems, integrating these genes into the zebrafish genome, and then characterizing that they indeed drive rhabdomyosarcoma tumor formation. (bvsalud.org)
These are located in a region of a few hundred kb that also contains 8 insertions of gene clusters for type III, IV, and V secretion systems, and genes for putatively secreted molecules such as cholera-like toxins. (scilifelab.se)
The phylogenies indicate a recent transfer of seven genes in the virB gene cluster for a type IV secretion system from a cat-adapted B. henselae to a dog-adapted B. vinsonii strain. (scilifelab.se)
According to the prediction accuracy of our models for incomplete genomes we recommend that at a major fraction (default = 85%) of the marker genes should be present in order to obtain reliable secretion system predictions. (effectivedb.org)
The chromosome encodes a complete type VI secretion system and plasmid genes encode type IV secretion proteins. (nottingham.ac.uk)
P. vortex's genome, for example, [32] harbors many genes which are employed in these strategies, in particular it has the potential to produce siderophores under iron-limiting conditions. (wikipedia.org)
More extensive genome sequencing could provide fundamental insights into pathways involved in complex social behavior of bacteria, and can discover a source of genes with biotechnological potential. (wikipedia.org)
Bacterial type I and type II secretion systems are required to trigger the induction of IG and JA-related genes while the type III secretion system appears to partially inhibit these defence pathways. (inrae.fr)
Barrier agains t pathogen s gut bacterial genes in the MetaHIT ation when attempting to extrapolate [10], although Proteobacteria, Ver- catalogue were also well represent- results obtained in mouse models to rucomicrobia, and Fusobacteria are ed in the other metagenomes that the situation in humans. (who.int)
In 2014, the MetaHIT consortium by bacteria of the genera Lacto- alytical method used: 66 from 16S published an integrated catalogue bacillus , Prevotella , and Sneathia , rDNA sequencing [11] or 57 from of 10 million bacterial genes de- whereas for infants delivered by whole-metagenome sequencing [5]. (who.int)
As a result of the rapid advances in genetics technology and the Human Genome Project, most of the estimated 100,000 genes in humans will be identified by the year 2005 (1). (cdc.gov)

This genome wide analysis allowed the identification of a set of 13 proteins constituting the T6SS protein core and a set of conserved accessory proteins. (biomedcentral.com)
Both systems export the Hcp ( H aemolysin- C oregulated P rotein) and presumably a class of proteins named Vgr ( V al- G ly R epeats), whose exact function is still speculative. (biomedcentral.com)
These three subcomplexes work together to transport proteins across the bacterial cell envelope and into a target cell through a contractile mechanism The phage tail-like component of the T6SS is a dynamic tubular structure that undergoes cycles of assembly and disassembly. (wikipedia.org)
Protein secretion systems are complex molecular machineries that translocate proteins through the outer membrane and sometimes through multiple other barriers. (bvsalud.org)
In this chapter, we describe a complete pipeline of analysis, starting from (i) the integration of information from a reference set of experimentally studied systems, (ii) the identification of conserved proteins and the construction of their HMM protein profiles, (iii) the definition and optimization of "macsy-models," and (iv) their use and online distribution as tools to search genomic data for secretion systems of interest. (bvsalud.org)
T3S assays performed with T3S-competent Y. enterocolitica or with an isogenic T3S-deficient strain individually expressing the TEM-1 fusions revealed a statistically significant higher level of secretion of 22 fusion proteins relative to RplJ20-TEM-1. (europa.eu)
Thirteen proteins showed a statistically significant higher level of secretion than full-length C. trachomatis RplJ. (europa.eu)
We also tested secretion of 11 out of these 13 proteins when expressed from an arabinose-inducible promoter in Y. enterocolitica, which revealed T3S-dependent secretion of 7 proteins. (europa.eu)
We found that gp21 and the herpesvirus proteases have similarity with proteins forming the tubes of phage tails and bacterial type VI secretion systems, suggesting their common evolutionary origin. (rcsb.org)
The new web portal provides access to predicted effector proteins and secretion systems for 1677 bacterial genomes and allows users to submit interactive calculation jobs. (univie.ac.at)
This method predicts type III, IV and VI secretion systems, which are all able to inject eukaryotic cells and to directly transfer proteins into the host cell cytosol. (effectivedb.org)
The interolog-based method predicts ~0.79M PPIs involving around 7700 Arabidopsis and 1068 Pseudomonas proteins in the full genome. (biomedcentral.com)
One of the major classes of virulence factors includes effector proteins that are delivered into the host through a type III protein secretion system (TTSS) to suppress plant immune responses, and also to facilitate disease development [ 6 ]. (biomedcentral.com)
Polar targeting of this T4BSS is mediated by two Dot/Icm proteins, DotU and IcmF, that are homologues of the T6SS membrane complex components TssL and TssM, suggesting that the Dot/Icm T4BSS is a hybrid system. (tcdb.org)
After FMT, fecal proteomics detected 301 proteins composed of 154 of human origin - mostly related to gut barrier protection - and 147 of bacterial origin - mostly enzymes - at days 7, 30, and up to 90. (medscape.com)

For decades, researchers have been fascinated by the three-dimensional structure and function of these nuclear pores, which act as guardians of the genome: substances that are required for controlling the cell are allowed to pass, while pathogens or other DNA-damaging substances are blocked from entry. (mpg.de)
Since then, it is estimated that at least a quarter of all pathogenic and non-pathogenic proteobacterial genomes encode for a T6SS, including pathogens of animals, plants, and humans, as well as soil, environmental or marine bacteria. (wikipedia.org)
Chlamydia trachomatis are obligate intracellular bacterial pathogens that cause ocular and genital infections, which are a significant public health concern worldwide. (europa.eu)
Recent advances in DNA sequencing technologies have reduced sequencing costs and hands-on time, and whole-genome sequencing of bacterial pathogens is being routinely performed by public health organizations. (biomedcentral.com)
Sequences from specific bacterial pathogens are also available as curated collections of genomic assemblies bundled with their metadata together with dedicated tools for population genomic analyses. (biomedcentral.com)
We mined the literature for experimental data on infectious dose of bacterial pathogens in humans (ID50) and also for traits with which ID50 might be associated. (pasteur.fr)
The induction of the IG pathway by bacterial pathogens has been reported several times in vitro. (inrae.fr)
The cathelicidin family of antimicrobial peptides is an integral component of the innate immune response that exhibits activity against bacterial, fungal, and viral pathogens. (medscape.com)
Each individ- cluding immune cells, but also the was previously cal ed the gastroin- ual harbours hundreds of different metabolic and nervous systems), testinal flora or microflora, the more species, most of which have not and protecting against pathogens pragmatic term "microbiota" is now yet been cultured. (who.int)

Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? (biomedcentral.com)
The availability of hundreds of bacterial genomes allowed a comparative genomic study of the Type VI Secretion System (T6SS), recently discovered as being involved in pathogenesis. (biomedcentral.com)
By combining comparative and phylogenetic approaches using more than 500 prokaryotic genomes, we characterized the global T6SS genetic structure in terms of conservation, evolution and genomic organization. (biomedcentral.com)
Bacterial DNA could be separated from host DNA based on genomic size. (bvsalud.org)
Nowadays, the production of full Wolbachia genomes does not require the physical isolation of the bacterial strains from their respective hosts, and the bacterium is often sequenced as a by-catch of host genomic projects. (bvsalud.org)
We sequenced and constructed the complete genome of an environmental strain CR1 of P. aeruginosa and performed the comparative genomic analysis. (frontiersin.org)
We identified eight new regions of genomic plasticity and a plasmid pCR1 with a VirB/D4 complex followed by trimeric auto-transporter that can induce virulence phenotype in the genome of strain CR1. (frontiersin.org)
The analysis of genomic sequences by phylogenetic approaches can yield insights into evolutionary distances for 1000s of bacterial genomes. (biomedcentral.com)
Genomic analysis of the type VI secretion systems in Pseudomonas spp. (sjtu.edu.cn)
Here, we examined the genetic diversity, distribution and mobilisation of iuc and iro loci amongst 2503 K . pneumoniae genomes using comparative genomics approaches and developed tools for tracking them via genomic surveillance. (biomedcentral.com)

Here, we sequenced 43 H. cinaedi- or H. canicola -like strains isolated from humans, hamsters, rats and dogs and collected 81 genome sequences of H. cinaedi , H. canicola and other enterohepatic Helicobacter strains from public databases. (microbiologyresearch.org)
Novel bacterial phyla are often represented by a handful of strains, and the number of species interacting with eukaryotes is likely underestimated. (nih.gov)
In this study, we performed genome analyses of more than 1,000 bifidobacterial strains publicly available revealing that Bifidobacterium adolescentis taxon might represent a model GABA producer in human gastrointestinal tract. (nature.com)
Recently, the term "psychobiotic" has been introduced to designate live bacterial strains, including lactobacilli and bifidobacteria, which are able to influence the CNS function 8 . (nature.com)
Among its various strains, pathovar tomato strain DC3000 ( PstDC3000 ) is asserted to infect the plant host Arabidopsis thaliana and thus, has been accepted as a model system for experimental characterization of the molecular dynamics of plant-pathogen interactions. (biomedcentral.com)

Identification of predicted pathogenesis and symbiosis determinants such as the Type III Secretion System (T3SS) in the genomes of "free-living" bacteria suggests that these microbes participate in uncharacterized interactions with eukaryotes. (nih.gov)
The central role of Type III secretion systems (T3SS) in bacteria-plant interactions is well established, yet unexpected findings are being uncovered through bacterial genome sequencing. (pasteur.fr)

A gene transfer agent and a dynamic repertoire of secretion systems hold the keys to the explosive radiation of the emerging pathogen Bartonella. (scilifelab.se)
describe a new mode of bacterial spread that appears to avoid the risk of extracellular exposure of the pathogen in a unique fashion. (biologists.com)
The authors discover a bacterial pathogen in the nematode Oscheius tipulae that adopts a filamentous form to spread across host intestinal cells. (biologists.com)
Both the host-pathogen model system used, and the identified mechanism of cell-to-cell spread are exciting and unique. (biologists.com)
Pseudomonas syringae is a major bacterial leaf pathogen that causes diseases in a wide range of plant species. (biomedcentral.com)
Hence, there is a need for developing efficient computational models to predict the interaction between host and pathogen in a genome scale, and find novel candidate effectors and/or their targets. (biomedcentral.com)
This can be a useful resource to the plant community to characterize the host-pathogen interaction in Arabidopsis and Pseudomonas system. (biomedcentral.com)

The upper lobe isolate, for instance, contains a SNP in the ciprofloxacin target topoisomerase parE and a SNP in exsD, a key negative regulator of the type 3 secretion system, which is among the most potent bacterial virulence factors. (genomeweb.com)
Infectious dose is not associated with genome size and shows insignificant phylogenetic inertia, in line with frequent virulence shifts associated with the horizontal gene transfer of a small number of virulence factors. (pasteur.fr)

The Icm/Dot or Dot/Icm multicomponent protein secretion system. (tcdb.org)

and strain typing methods from serologic to whole-genome sequencing. (cdc.gov)
The chapter on non-whole-genome sequencing methods for strain typing for epidemiologic investigation is well done and could be of interest for those trying to dissect the older literature. (cdc.gov)
The increasing accessibility of whole-genome sequencing across research and clinical settings has improved our ability to predict antibacterial susceptibility, to track epidemics at the level of individual outbreaks and wider historical trends, to query the efficacy of the bacille Calmette-Guérin (BCG) vaccine, and to uncover targets for novel antitubercular therapeutics. (jci.org)
Whole-genome sequencing (WGS) of M. tuberculosis and related mycobacteria is now routine, allowing comparisons across time and space. (jci.org)

Complex interactions between Gram-negative bacteria and their environment are facilitated by numerous surface-attached and exported macromolecules, some of which represent bacterial toxins and effectors. (biomedcentral.com)
citation needed] Although the ancestral function of the T6SS appears to be targeting of bacteria, a handful of systems have been identified that have evolved to target eukaryotic cells. (wikipedia.org)
It can be used with a set of predefined MacSyFinder models, "TXSScan," to identify all major secretion systems of diderm bacteria (i.e., with inner and LPS-containing outer membranes) as well as evolutionarily related cell appendages (pili and flagella). (bvsalud.org)
bacteria: The colony diameter is 5 cm and the colors indicate the bacterial density (bright yellow for high density). (wikipedia.org)
To fulfill their requirements for iron, bacteria have developed several strategies, including the reduction of ferric to ferrous ions, the secretion of high-affinity iron-chelating compounds, called siderophores , and the uptake of heterologous siderophores. (wikipedia.org)
Edwards explained that FMT modifies the gut microbiome, which metabolically reprograms by replenishing enzymes sourced from commensal bacteria that were previously depleted, causing a rise in ammonia metabolism such that it produces energy for the immune system and gut barrier. (medscape.com)

In this study, we undertook extensive genome-wide comparative analyses of twelve species that conform the Pectobacterium genus. (mdpi.com)
These compilations were checked and complemented with genome analyses. (pasteur.fr)

Recent reports demonstrated the importance of T6SS in pathogenesis of several bacterial species. (biomedcentral.com)
The type VI secretion system (T6SS) is molecular machine used by a wide range of Gram-negative bacterial species to transport effectors from the interior (cytoplasm or cytosol) of a bacterial cell across the cellular envelope into an adjacent target cell. (wikipedia.org)
Bacterial species can also be motile and employ various means to accomplish this such as flagella. (wikibooks.org)
With the genotype list as input, the three models calculate binary classifications whether the input species contains functional Type III, IV and VI secretion systems. (effectivedb.org)
Genome sequencing found that FMT increased the richness of species with significant donor engraftment. (medscape.com)
The microbiome, the "other ge- species have no representative in nome" or "second genome" of the culture collections. (who.int)
A catalogue of 178 reference bacterial birth, and the delivery type is the first core of species has been identified genomes distributed among different factor that has an impact. (who.int)

Using this method, we successfully identified multiple HCCM complex-containing human faecal metagenome samples and obtained the sequence information covering almost the entire genome of each strain. (microbiologyresearch.org)
However, the microbial metabolic pathway of the fungicide and the genetic systems driving its degradation by strain P3 remain unknown. (nature.com)
Among them, pathovar tomato strain DC3000 ( Pst DC3000 ) has been asserted to infect the plant host Arabidopsis thaliana and tomato causing bacterial spec and brown spot. (biomedcentral.com)

the complete genome sequence of Mycobacterium tuberculosis continues to provide an invaluable resource to understand tuberculosis (TB), the leading cause of global infectious disease mortality. (jci.org)

Interestingly, staging the infection cycle in worms by pulse-chase experiments revealed the presence of short and long intracellular bacterial filaments at 16- and 24-hours post-infection (hpi) whereas by 38 and 48 hpi most worms contained only the coccobacilli form. (biologists.com)

These effectors manipulate host cell processes to enable e. g. bacterial invasion, acquisition of nutrients, inhibition of apoptosis, or interference with immune signalling. (europa.eu)
Legionella pneumophila survives and replicates inside host cells by secreting ~300 effectors through the defective in organelle trafficking (Dot)/intracellular multiplication (Icm) type IVB secretion system (T4BSS). (tcdb.org)

In V. cholera e, three Vgrs (VgrG1-3) are encoded in the genome, and are exported in a T6SS-dependent way. (biomedcentral.com)
The T6SS is thought to resemble an inverted phage extending outward from the bacterial cell surface. (wikipedia.org)
Unlike substrates of other secretion systems (such as the general secretory pathway or secretion systems III and IV), those of the T6SS are not known to have any universally identifying features. (wikipedia.org)
The T6SS of Vibrio cholerae has a dual role, being able to target both bacterial and eukaryotic cells. (wikipedia.org)

One recent k -mer-based method, BIGSI, employs a data structure that stores a Bloom filter [ 11 ] of k -mers for each genome in a database, and can subsequently index and search very large databases of bacterial and viral sequences [ 12 ]. (biomedcentral.com)
A symptomatic person is most contagious for the several days before and after the onset of symptoms, at which time the viral load in respiratory secretions is greatest. (msdmanuals.com)

The baseplate and phage tail-like complex interact in the bacterial cytoplasm, and then are recruited to the cell envelope by the membrane complex. (wikipedia.org)
Members of the second class of substrates are targeted for secretion via interaction with the phage tail spike-like protein VgrG. (wikipedia.org)
Analysis of the Campylobacter phage CP8 genome identified significant sequence identity of protein gp010 with the avian leukocyte receptor CD30 ligand. (nottingham.ac.uk)

Among 22 tested compounds six block E-cadherin cleavage by HtrA in vitro and result in reduced scattering and wound healing of gastric epithelial cells, thereby preventing bacterial infiltration of the epithelium. (uni-frankfurt.de)

New sequencing data are now routinely uploaded to public databases such as the Sequence Read Archives (SRA [ 6 ]), which provide ready access to extensive collections of sequencing data for many bacterial genera. (biomedcentral.com)
Furthermore, BIGSI was designed for dealing with genetically diverse collections of data, and other methods and different data structures might be more efficient for creating a query index of sequence data from closely related genomes. (biomedcentral.com)
While the genome sequence was already transformative at the time, the past 25 years of progress have substantially increased its impact on TB taxonomy, drug discovery, resistance mechanisms, epidemiology, vaccine development, and pathogenesis. (jci.org)

Evolutionary dynamics of pathoadaptation revealed by three independent acquisitions of the VirB/D4 type IV secretion system in Bartonella. (cdc.gov)

I also like that the study includes the isolation of a filamentation-defective mutant that points to a metabolic pathway and molecular players that could regulate this process of bacterial spread. (biologists.com)

Conclusions/Significance: This study demonstrates that receptor-based virtual screening with a permissive ('fuzzy') pharmacophore model can help identify small bioactive agents for combating bacterial infection. (uni-frankfurt.de)
Our results show the key role of immune subversion in effective human infection by small bacterial populations. (pasteur.fr)
We performed a genome-wide analysis of the Arabidopsis thaliana transcriptome during D. dadantii infection and conducted a genetic study of identified responses. (inrae.fr)
Together, these disrupt healthy functioning of the immune system, which increases susceptibility to infection and mortality. (medscape.com)

cytoplasmic loop= residues 90-94 inside) during biogenesis of the Agrobacterium VirB/VirD4 type IV secretion system. (tcdb.org)

Peritoneal stomata constitute the principal pathways for the drainage of intraperitoneal contents from the PERITONEAL CAVITY to the LYMPHATIC SYSTEM. (bvsalud.org)

First evidence of bacterial translocation from the intestinal tract as a route of Helicobacter cinaedi bacteremia. (microbiologyresearch.org)
Patients with cirrhosis have an underabundance of commensal microbes, an overabundance of pathogenic microbes, and reduced gut microbiome diversity, as well as gut barrier damage and bacterial translocation. (medscape.com)

Motility: Structure and function of flagella, and control via chemotaxis systems. (manchester.ac.uk)

Missing from the book, presumably by design, are a chapter reviewing in detail the ecology of the bacterium in the built environment, practical guidance on outbreak investigation, advanced techniques in epidemiologic source investigation, molecular and cellular pathogenesis other than secretion systems, and the molecular evolution of the bacterium, all of which can be found in other sources. (cdc.gov)

It is believed to span from the inner membrane to the outer membrane of the Gram negative bacterial cell envelope, forming a channel that opens and closes with a unique iris-like mechanism. (wikipedia.org)

BlastFrost is a highly efficient method for querying 100,000s of genome assemblies, building on Bifrost, a dynamic data structure for compacted and colored de Bruijn graphs. (biomedcentral.com)

The "EffectiveS346" software has been implemented for the Effective database in order to predict secretion systems encoded in bacterial genomes and to provide a clear "yes/no" prediction on whether they are sufficiently complete or not. (effectivedb.org)

Gastric acid is a key factor in normal upper gastrointestinal functions, including protein digestion and calcium and iron absorption, as well as providing some protection against bacterial infections. (genome.jp)

The major burden of iuc and iro amongst the genomes analysed was due to two linked lineages ( iuc1 / iro1 74% and iuc2 / iro2 14%), each carried by a distinct non-self-transmissible IncFIB K virulence plasmid type that we designate KpVP-1 and KpVP-2. (biomedcentral.com)
These MGEs, most typically plasmids and integrative and conjugative elements (ICEs), are therefore important constituents of the accessory genome that imbue K . pneumoniae organisms with their distinct HA or CA clinical profiles. (biomedcentral.com)
The genome atlases allowed for distinguishing distinct DNA structures and highlighted suspicious high transcription zones. (mdpi.com)
Based on their finding of strong signals of genetic compartmentalization, regional isolates with similar protein expression profiles, and spatially distinct distribution of bacterial phenotypes, the researchers said that their study indicates that isolation in different lung regions helped drive the diversification of P. aeruginosa in the lungs of cystic fibrosis patients. (genomeweb.com)

You will also be introduced to the key emerging technological developments, such as next generation sequencing and the concept of the microbiome as well as the role of CRISPR as a bacterial defence system. (manchester.ac.uk)
Silver Age of GOLD Introduces New Features The Genomes OnLine Database makes curated microbiome metadata that follows community standards freely available and enables large-scale comparative genomics analysis initiatives. (doe.gov)

The researchers used phenotypic data to classify the isolates into subpopulations, and found that each patient harbored between 12 and 40 bacterial subpopulations in their lungs and that each lobar region contained between one and three of those sub-populations. (genomeweb.com)
After sequencing the genomes of 96 isolates from the upper, middle, and lower lobes from three patients, the researchers constructed phylogenetic trees. (genomeweb.com)

Since the first release of the P. aeruginosa PAO1 genome, there are more than 5700 genomes published. (bvsalud.org)
In this chapter, we give a brief historical overview of the knowledge gained from P. aeruginosa genome sequencing, then we describe the wet-lab procedure to extract the DNA and prepare the library for broad genome sequencing using Illumina MiSeq technology. (bvsalud.org)
Using 16S rRNA sequencing, they determined the bacterial genera present in the samples, finding the vast majority to be P. aeruginosa . (genomeweb.com)

I found that several of the chapters contained quite useful information that would be hard to find elsewhere, including a thorough review of L. pneumophila virulence secretory systems, as well as a review of the freshwater ecology of the bacterium, the clinical microbiology and clinical significance of Legionella spp. (cdc.gov)

Trw conjugation system of E. coli plasmid IncP. (tcdb.org)

Finally, we will invite speakers to talk about aspects of personalized or stratified medicine based on systems biology approaches for better care and future drug development. (lu.se)

The input data for EffectiveS346 are genotype files listing COGs and NOGs according to EggNOG 4.0, which are present in a particular genome. (effectivedb.org)

We hope this simple protocol will support many projects aiming at studying diverse Wolbachia genome assemblies. (bvsalud.org)
BACKGROUND: While genome-resolved metagenomics has revolutionized our understanding of microbial and genetic diversity in environmental samples, assemblies of short-reads often result in incomplete and/or highly fragmented metagenome-assembled genomes (MAGs), hampering in-depth genomics. (bvsalud.org)

At the 25-year anniversary of this accomplishment, we describe how insights gleaned from the M. tuberculosis genome have led to vital tools for TB research, epidemiology, and clinical practice. (jci.org)

In order to cross two bacterial membranes, those molecules are transported by nanomachineries, called secretion systems, which may be more or less complex in terms of their composition and regulation. (biomedcentral.com)

Simultaneous ALK3 and ALK2 Inhibition Prevents hMSCs from Bone tissue Remodeling by Activating a Protective System Against Vascularization. (bakingandbakingscience.com)

Contraction of the sheath is thought to propel the Hcp tube, VgrG and associated substrates outside of the bacterial cell, where the VgrG/PAAR spike facilitates penetration of the membrane of a neighboring cell. (wikipedia.org)

KEGG GENOME: Stenotrophomonas sp. (kegg.jp)
EffectiveS346 additionally provides lists of the 100 most significant COGs in regard to each classification and lists of those COGs that are contained in the KEGG maps of the three secretion systems. (effectivedb.org)

We also developed a method to systematically search for H. cinaedi sequences in large metagenome data sets based on the results of genome comparison. (microbiologyresearch.org)
These approaches were implemented because k -mers can directly identify diverse genetic modifications such as single nucleotide polymorphisms (SNPs), insertions or deletions from short read sequences, and do not require assembled genomes or an explicit reference genome. (biomedcentral.com)

The genome of C. coli OR12 was sequenced to identify factors which contribute to its fitness in colonizing chickens. (nottingham.ac.uk)

What made this so important to us is that the bacterial populations inhabiting different lung regions varied dramatically in terms of their antibiotic resistance and virulence," first author Peter Jorth from the University of Washington School of Medicine said in a statement. (genomeweb.com)
Through a proteomic analysis, the researchers found that regional bacterial populations show protein expression differences. (genomeweb.com)

using electron cryotomography mapped the location of the core and accessory components of the Legionella core transmembrane subcomplex, revealing a well-ordered central channel that opens into a large, windowed secretion chamber with an unusual 13-fold symmetry. (tcdb.org)

Our results suggest that trade-offs between selection for population growth-related traits and selection for the ability to subvert the immune system shape bacterial infectiousness. (pasteur.fr)
For patient education information, see the Immune System Center and Sexually Transmitted Diseases Center , as well as HIV/AIDS . (medscape.com)

Increasing bacterial resistance against antibiotics demands for innovative strategies for therapeutic intervention. (uni-frankfurt.de)

The effect of the unregulated increase in GDH activity on insulin secretion was examined by overexpressing GDH266C in an insulinoma cell line, MIN6. (diabetesjournals.org)
Although glutamine alone did not stimulate insulin secretion from control MIN6-lacZ, it remarkably stimulated insulin secretion from MIN6-GDH266C. (diabetesjournals.org)
Interestingly, insulin secretion is also exaggerated significantly at low glucose concentrations (2 and 5 mmol/l) but not at higher glucose concentrations (8-25 mmol/l). (diabetesjournals.org)
Our results directly illustrate the importance of GDH in the regulation of insulin secretion from pancreatic β-cells. (diabetesjournals.org)

TXSScan models can be altered in the command line or customized to search for variants of known secretion systems. (bvsalud.org)

Organisms2

Chemicals and Drugs2

Phenomena and Processes5

Information Science1

Genes19

Proteins15

Pathogens9

Genomic10

Strains5

T3SS2

Pathogen7

Virulence Factors2

Protein secretion system1

Whole-genome4

Bacteria6

Analyses2

Species7

Strain3

Complete genome1

Intracellular bacterial1

Effectors2

T6SS4

Viral2

Phage3

Epithelial cells1

Sequence3

Bartonella1

Pathway1

Infection4

VirB1

Pathways1

Translocation2

Chemotaxis1

Molecular1

Outer membrane1

100,0001

Prediction1

Gastric1

Distinct4

Microbiome2

Isolates2

Aeruginosa3

Secretory1

Plasmid1

Biology1

Genotype1

Assemblies2

Insights1

Membranes1

Inhibition1

Substrates1

KEGG2

Sequences2

Coli1

Populations2

Channel that opens1

Immune system2

Resistance1

Insulin4

Variants1