• The inter- and intra- specific SNPs identified from all catfish EST dataset assembly will greatly benefit the catfish introgression breeding selection and whole genome association studies. (auburn.edu)
  • EST approaches have largely been superseded by whole genome and transcriptome sequencing and metagenome sequencing. (wikipedia.org)
  • Sequencing of the A. oryzae genome was accomplished using the whole-genome shotgun (WGS) approach. (nature.com)
  • Phylogenetic analysis of the three aspergilli using the whole-genome data showed that A. nidulans branched off earlier than A. oryzae and A. fumigatus 5 . (nature.com)
  • When available, the information provided by whole-genome sequencing projects provides an entry into an understanding of genome structure and evolution and gene discovery, and function. (biomedcentral.com)
  • The system is capable of expressed sequence tag (EST) sequencing, the identification of small RNAs and transcription binding sites as well as whole genome sequencing and metagenomics. (outsourcing-pharma.com)
  • One of the things that is really important for high throughput DNA sequencing is for people to see that researchers can look at the whole genome of bacteria and viruses. (outsourcing-pharma.com)
  • The advent of low cost and high accuracy exome and whole genome sequencing has led many global healthcare organizations to adopt genome sequencing early in managing patient populations. (cdc.gov)
  • High-Quality Draft Genome Sequences for Four Drug-Resistant or Outbreak-Associated Shigella sonnei Strains Generated with PacBio Sequencing and Whole-Genome Maps. (cdc.gov)
  • Revisiting the taxonomy of the genus Elizabethkingia using whole-genome sequencing, optical mapping, and MALDI-TOF, along with proposal of three novel Elizabethkingia species: Elizabethkingia bruuniana sp. (cdc.gov)
  • Of these unique sequences, over 35% had significant similarities to known genes by BLASTX searches, which allowed the identification of 14,776 unique genes in the catfish. (auburn.edu)
  • A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat. (bvsalud.org)
  • Because these clones consist of DNA that is complementary to mRNA, the ESTs represent portions of expressed genes. (wikipedia.org)
  • Some authors use the term "EST" to describe genes for which little or no further information exists besides the tag. (wikipedia.org)
  • Here we describe a genome-wide effort, carried out as part of the Mammalian Gene Collection (MGC) project, to identify human genes not yet in the gene catalogs. (nih.gov)
  • Our approach was to produce gene predictions by algorithms that rely on comparative sequence data but do not require direct cDNA evidence, then to test predicted novel genes by RT-PCR. (nih.gov)
  • The NGFs appear to be predominantly protein-coding genes rather than noncoding RNAs, unlike novel transcribed sequences identified by technologies such as tiling arrays and CAGE. (nih.gov)
  • Our results demonstrate that many important genes and gene fragments have been missed by traditional approaches to gene discovery but can be identified by their evolutionary signatures using comparative sequence data. (nih.gov)
  • use microarray experiments to conclude that the human genome project will uncover many genes not previously discovered by EST sequencing (Nat Genet 2000, 26:315-318). (the-scientist.com)
  • Here we show that the 37-megabase (Mb) genome of A. oryzae contains 12,074 genes and is expanded by 7-9 Mb in comparison with the genomes of Aspergillus nidulans 5 and Aspergillus fumigatus 6 . (nature.com)
  • The blocks of A. oryzae -specific sequence are enriched for genes involved in metabolism, particularly those for the synthesis of secondary metabolites. (nature.com)
  • The 37-Mb genome was predicted to contain a total of 12,074 genes encoding proteins with a length greater than 100 amino acid residues (see Methods). (nature.com)
  • using a redundancy criterion to identify reproducible sequence differences between related genes within gene families. (biomedcentral.com)
  • Analysis of these sequences revealed single base substitutions and single base indels are the most frequently observed form of sequence variation between genes within families in the dataset. (biomedcentral.com)
  • Functional annotation of gene sequences indicate functional classifications are not randomly distributed among gene families containing few or many genes. (biomedcentral.com)
  • The more recent duplication event in particular would be expected to result in many paralogous pairs of genes differing by relatively few sequence differences, thus complicating gene identification using ESTs. (biomedcentral.com)
  • Genetic improvement of this important crop depends on a complete and through understanding of the genes that make up the genome and how they have evolved. (usda.gov)
  • A first step to understanding the soybean genome is to identify all genes in the genome and then to infer functions for them in order to identify genes that may play a role in traits of agronomic importance. (usda.gov)
  • We also identified several genes that do not appear to be protein coding, and generated 17 kb of novel transcript sequence data from re-sequencing 97 EST clones. (biomedcentral.com)
  • Targeted integration by homologous recombination enables in-situ tagging and replacement of genes in the marine microeukaryote Diplonema papillatum . (umontreal.ca)
  • Unusual mitochondrial genomes and genes. (umontreal.ca)
  • Application of disease-associated differentially expressed genes--mining for functional candidate genes for mastitis resistance in cattle. (fbn-dummerstorf.de)
  • Identification and chromosome assignment of 23 genes expressed in meat and dairy cattle. (fbn-dummerstorf.de)
  • Trait-associated expressed genes - Mining for functional candidate genes in cattle. (fbn-dummerstorf.de)
  • In this study, a suppression subtractive hybridization cDNA library approach was used for the identification of pathogen and plant genes that were differentially expressed during infection of the susceptible cultivar BRS Pérola of Phaseolus vulgaris L. A total of 979 unigenes (430 contigs and 549 singletons) were obtained and classified according to their functional categories. (frontiersin.org)
  • and he Began to use it to Identify genes welke are Expressed in the human brain. (multi-mania.be)
  • 10,200 protein-encoding genes were predicted based on the genome sequence. (springeropen.com)
  • The genes and proteins were predicted and annotated based on the genome sequence. (springeropen.com)
  • We have shown that approximately 14% of the newly sequencedexpressed sequence tags map completely or partially to genomic regions where there are no annotated genes orsplice variants and therefore, imply that these are novel genetic structures. (sfu.ca)
  • Expressed sequence tags, which mapto intergenic (around 1000) and intronic regions (around 580), may represent novel transcribed regions, such asunannotated or unrecognized small protein-coding or non-protein-coding genes or splice variants. (sfu.ca)
  • Primary annotations based on sequence similarity are linked to networks of systematic annotation in Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) and can be queried and computationally utilized in downstream analyses. (umsystem.edu)
  • With a good resource of ESTs, a large number of molecular markers can be identified, and issues related to alternative splicing and differential poly adenylation can be addressed at the genome-wide scale. (auburn.edu)
  • A total of 600,000 sequencing attempts were made, generating a total of 438,321 quality ESTs. (auburn.edu)
  • In order to select reliable SNPs, contigs containing four or more ESTs should be used and the minor allele sequence should be represented at least twice. (auburn.edu)
  • Application of such quality assessment measures, along with large resources of ESTs, should provide effective means for SNP identification in species where genome sequence resources are lacking. (auburn.edu)
  • The chromosome bin map of ESTs is a unique resource for SNP analysis , comparative mapping, structural and functional analysis , and polyploid evolution, as well as providing a framework for constructing a sequence-ready, BAC-contig map of the wheat genome . (bvsalud.org)
  • ESTs may be used to identify gene transcripts, and were instrumental in gene discovery and in gene-sequence determination. (wikipedia.org)
  • Because of the way ESTs are sequenced, many distinct expressed sequence tags are often partial sequences that correspond to the same mRNA of an organism. (wikipedia.org)
  • When the complete genome sequence of an organism is available and transcripts are annotated, it is possible to bypass contig assembly and directly match transcripts with ESTs. (wikipedia.org)
  • Expressed sequence tags (ESTs) have given researchers a quick if dirty look at the coding potential of the human genome. (the-scientist.com)
  • A total of 35 microsatellites were identified from the ESTs, with 18 having sufficient flanking sequences for primer design. (nih.gov)
  • A total of 5,542 ESTs were generated representing 4,688 unique sequences. (nih.gov)
  • The short cDNA sequence Fragments when making this method are called expressed sequence tags , or ESTs. (multi-mania.be)
  • Results We describe a bioinformatics pipeline known as FunnyBase that has been used to store, annotate, and analyze 40,363 expressed sequence tags (ESTs) from the heart and liver of the fish, Fundulus heteroclitus. (umsystem.edu)
  • The genome was confirmed to comprise eight chromosomes (chromosomes 1-8 in decreasing size), the assignment of which is different from a previous report 7 ( Supplementary Table S1 and schematic drawing in Supplementary Fig. S1 ). (nature.com)
  • An examination of the content and distribution of repetitive DNA sequences to identify the location of such sequences within specific chromosomes and their conservation across species of the Pinaceae family. (le.ac.uk)
  • Fluorescent in situ hybridisation (FISH) on metaphase chromosomes and on extended chromatin will be used to physically locate repetitive as well as single copy DNA sequences on conifer chromosomes and to integrate physical and genetic maps. (le.ac.uk)
  • Comparative mapping of five coding DNA sequences on cattle chromosomes 7 and 25. (fbn-dummerstorf.de)
  • In the nineteenth and twentieth centuries, the development of new microscopic and molecular techniques, including DNA sequencing, enabled scientists to confirm the hypothesis that chromosomes determine the sex of developing organisms. (asu.edu)
  • Array comparative genomic hybridization (aCGH) is a type of microarray now routinely used to identify deleted or duplicated regions of DNA sequence in specific chromosomes on a genome-wide basis. (msdmanuals.com)
  • Silver Age of GOLD Introduces New Features The Genomes OnLine Database makes curated microbiome metadata that follows community standards freely available and enables large-scale comparative genomics analysis initiatives. (doe.gov)
  • In November, 2017, the NASEM Roundtable on Genomics and Precision Health held the workshop, " Implementing and Evaluating Genomic Screening Programs in Health Care Systems , " which explored the emerging landscape of worldwide organizations that are (or soon will be) applying genome sequencing as a screening tool in populations. (cdc.gov)
  • A complete and accurate set of human protein-coding gene annotations is perhaps the single most important resource for genomic research after the human-genome sequence itself, yet the major gene catalogs remain incomplete and imperfect. (nih.gov)
  • Large scale gene analysis of most organisms is hampered by incomplete genomic sequences. (biomedcentral.com)
  • The identification of potential gene sequences (pHaps) from soybean allows us to begin to get a picture of the genomic history of the organism as well as begin to observe the evolutionary fates of gene copies in this highly duplicated genome. (biomedcentral.com)
  • Genomic sequencing of selected loci indicate that intron-like intervening sequences are numerous and are approximately 220 bp in length. (biomedcentral.com)
  • Such EST data form a valuable foundation for the understanding of the gene composition and genomic biology of yet-to-be fully sequenced genomes [ 3 ]. (biomedcentral.com)
  • These processes have left footprints in the genomic sequence that can be detected by bioinformatic methods and may help to interpret receptor function in the light of a given species in its environment. (springer.com)
  • Future research programs can be focused on the development of transgenic plants with enhanced stress tolerance in field conditions based upon the outcome of genomic approaches and knowing the mystery of nucleotides sequences hidden in cells. (intechopen.com)
  • We have catalogued the protein kinase complement of the human genome (the "kinome") using public and proprietary genomic, complementary DNA, and expressed sequence tag (EST) sequences. (nordiag.com)
  • Additionally, attendees broadly expressed the perspective that health care disparities for genomic applications in underserved populations are an existing and expanding issue no matter the level of evidence of clinical utility. (cdc.gov)
  • During the nucleotide flow each of the beads is sequenced in parallel, with the polymerase extending the sequencing strand only if the nucleotide is complimentary to the template strand. (outsourcing-pharma.com)
  • If you sequence more than one nucleotide at once you get a stronger signal and we see a very linear response between the signal strength and the number of nucleotides added. (outsourcing-pharma.com)
  • Keywords: difference between the observed and analytically calculated expected SPER, expressed sequence tag, Illumina Genome Analyzer II, nucleotide, paired-end, ratio of empirically computed SPERs, mRNA sequencing, reads per kilobase of exon model per million mapped reads, supportive PE reads, University of California Santa Cruz. (tufts.edu)
  • Nucleotide sequences located at the ends of EXONS and recognized in pre-messenger RNA by SPLICEOSOMES. (bvsalud.org)
  • This work alsodemonstrates the successful and efficient application of a next generation sequencing methodology.Results: We have generated over 30 million bases of novel expressed sequence tags from first larval stage wormsutilizing high-throughput sequencing technology. (sfu.ca)
  • Suchapplication of this new sequencing technique allows for high-throughput, genome-wide experimental verificationof known and novel transcripts. (sfu.ca)
  • This large scale EST sequencing project would allow the identification of majority of catfish transcriptome. (auburn.edu)
  • Comparison of the three aspergilli species revealed the presence of syntenic blocks and A. oryzae -specific blocks (lacking synteny with A. nidulans and A. fumigatus ) in a mosaic manner throughout the genome of A. oryzae . (nature.com)
  • However, large-scale expressed sequence tag (EST) resources have been lacking for this species. (nih.gov)
  • Unfortunately, the size and complexity of the genomes of many agronomically important species currently hinders the undertaking of such projects. (biomedcentral.com)
  • Genetic studies in this organism are not practical because of the tetraploid nature of the genome and the long generation time of this species. (biomedcentral.com)
  • Two features of X. laevis that have hindered genetic studies are the long generation time (1 to 2 years) and the tetraploid genome of this species. (biomedcentral.com)
  • Transposons have widely been used in plant and invertebrate model species to integrate foreign DNA into the host genome. (biomedcentral.com)
  • Furthermore, other near-human species such as Zeltron, Chiss , and Sullustan share the human ortholog of this locus, which supports the inference that the ancestral sequence was noncoding, and that the GLABR has de novo origins in the Wookiee. (ubc.ca)
  • As Wookiees are a humanoid species, we postulate that when an all-against-all comparison is done with the Wookiee wookiee and Homo Sapien genomes, a portion of the Wookie genome will be distinct. (ubc.ca)
  • Otherwise, the absence of the phenotypic characteristics of plausible orthologs was attributed to the theorized inactivation within the genomes of other humanoid species. (ubc.ca)
  • This thematic series of companion papers constitutes the initial exploration of the genome biology of the waterflea (Daphnia) building on the first complete crustacean genome sequence. (biomedcentral.com)
  • Systematic sequencing of cDNA clones derived from xylem and leaf tissue will produce a new generation of research tools for conifer biology. (le.ac.uk)
  • In: Expressed sequence tags - generation and analysis, Methods in Molecular Biology Vol. 533 (J. Parkinson, ed. (umontreal.ca)
  • Genome Biology 11, no. 12 (10, 2010): 1-19. (tufts.edu)
  • Researchers from several institutions and different disciplines, ranging from biology to medicine and including bioinformatics, which was virtually unknown at the time, worked together in a large virtual network that brought together as many as 60 laboratories to face a shared objective: the genetic sequencing of several organisms. (fapesp.br)
  • Genome Res 10:344-349 (2000). (karger.com)
  • An initiative launched in 1997, exactly 10 years ago, it obtained its first international acknowledgement by means of the publication of an article on the cover of the journal Nature about the sequencing of the genetic code of the bacterium Xylella fastidiosa , which causes citrus variegated chlorosis, and attacks orange plantations (v. 406, n. 6.792, 13 July 2000). (fapesp.br)
  • In 1982, the idea of selecting random or semi-random clones from such a cDNA library for sequencing was explored by Greg Sutcliffe and coworkers. (wikipedia.org)
  • sequenced 178 clones from a rabbit muscle cDNA library. (wikipedia.org)
  • Single pass sequencing of cDNA clones provides a rapid route to gene discovery and production of EST's (2). (le.ac.uk)
  • A total of 6,528 clones were sequenced from this library generating 5,542 high-quality EST sequences. (nih.gov)
  • Partial cDNA (DNA, COMPLEMENTARY) sequences that are unique to the cDNAs from which they were derived. (ouhsc.edu)
  • The tagged DNA binds to its complementary DNA segment and can be detected by measuring the amount and type of fluorescence. (msdmanuals.com)
  • For those who already had experience in this, the target was learning to do an entire genome. (fapesp.br)
  • Using aCGH, the entire genome can be tested (queried). (msdmanuals.com)
  • This technology involves breaking the entire genome into small segments, sequencing the segments, and then reassembling the sequences using intensive computational techniques to provide the base-by-base sequence of the entire genome or more limited regions, such as the expressed portion of the genome known as the exome. (msdmanuals.com)
  • In 1991, Adams and co-workers coined the term EST and initiated more systematic sequencing as a project (starting with 600 brain cDNAs). (wikipedia.org)
  • However, the sheer volume of information generated by sequencing the exome or genome results in a variety of interpretive problems that complicate understanding of the results. (msdmanuals.com)
  • In genetics, an expressed sequence tag (EST) is a short sub-sequence of a cDNA sequence. (wikipedia.org)
  • In: Organelle Genetics 2012, Part 2: evolution of organelle genomes and gene expression (C. Bullerwell, ed), Springer-Verlag Heidelberg, Germany, p. 41-77. (umontreal.ca)
  • An extensive literature documenting physiological and ecological diversity make Daphnia an ideal model to study the genetic architecture of phenotypic variation in natural populations and the evolution of genome structure/function relationships in aquatic environments. (biomedcentral.com)
  • Segregating populations will be characterised with both single locus (microsatellites) and multi-locus (AFLPs) marker systems providing framework maps for the eventual location of cDNAs generated from single pass sequencing. (le.ac.uk)
  • Beginning in the 1980s, many studies of human populations used the Y-chromosome gene sequences to trace paternal lineages. (asu.edu)
  • DNA microarrays can be used in genome-wide association studies (GWAS) to compare patient and control populations to identify DNA variants that can contribute to disease risk. (msdmanuals.com)
  • We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
  • We envisioned that enzyme-activatable cMOs could overcome these limitations, as the triggering enzymes could be expressed in individual cell populations using cis regulatory elements. (cdc.gov)
  • We identified approximately 45,000 potential gene sequences (pHaps) from EST sequences of Williams/Williams82, an inbred genotype of soybean ( Glycine max L. Merr. (biomedcentral.com)
  • This study examined potential gene sequences identified in EST libraries of soybean and assigned the potential gene sequences with molecular functions. (usda.gov)
  • Abstract: We have developed FusionSeq to identify fusion transcripts from paired-end RNA-sequencing. (tufts.edu)
  • Expressedsequence tags, which map across intron-exon boundaries (around 300), indicate possible alternative splice sites,while expressed sequence tags, which map near the ends of known transcripts (around 600), suggest extensionof the coding or untranslated regions. (sfu.ca)
  • Approximately one half of the ORFs are expressed in at least some of the ten tissues and cell types queried by the microarray. (the-scientist.com)
  • These genome resources provide the material basis for future microarray development, marker validation, and genetic linkage and QTL analysis. (nih.gov)
  • The Pig Microarray is produced with the QIAGEN Array-Ready Oligo Set for the Pig Genome (version 1.0) and the Pig Genome Oligo Extension Set (version 1.0). (iastate.edu)
  • Each 13,000 element oligo array is printed on one single slide at the University of Minnesota Microarray Printing Facility and distributed through the U.S. Pig Genome Coordination Program. (iastate.edu)
  • They scan 350 Mb of finished and draft human sequence using three different gene-finding algorithms. (the-scientist.com)
  • The Pig Genome Oligo Set contains 10,665 70-mer probes representing (10,665) Sus Scrofa gene sequences with a hit to human, mouse, or pig gene transcript. (iastate.edu)
  • Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
  • For his vision in using expressed sequence tags in genetic analyses, his unique contributions to the sequencing of microbial genomes, and his leadership in mobilizing new methods and resources for sequencing the human genome. (nasonline.org)
  • Venter believed therein shotgun sequencing was the fastest and must effective way to get useful human genome data. (multi-mania.be)
  • [23] The method was rejected by the Human Genome Project however, since some geneticists fact represented it mention anything not be accurate enough for a genome as complicated as dat of humans, dat it mention anything be logistically more s difficult, and therein it mention anything about cost significantly more. (multi-mania.be)
  • [26] The goal of the company was to sequence the entire human genome and release it into tje public domain for non-commercial use in much less time and for much less cost dan the public human genome project. (multi-mania.be)
  • 1111, Yata, Mishima, Shizuoka 411-8540, Japan REFERENCE 2 AUTHORS Mishima,H., Shizuoka,T. and Fuji,I. TITLE Glyceraldehyde-3-phosphate dehydrogenase expressed in human liver JOURNAL Unpublished (2002) COMMENT Human cDNA sequencing project. (nig.ac.jp)
  • The protein kinase complement of the human genome. (nordiag.com)
  • A haplotype map of the human genome. (nordiag.com)
  • Recent advances in sequencing technology make it possible to comprehensively catalog genetic variation in population samples, creating a foundation for understanding human disease, ancestry and evolution. (nordiag.com)
  • Although the notion that malaria has helped shape the human genome is well- accepted, the lack of a nucleus in human erythrocytes has hindered our ability to study genetic interactions between these unusual host cells and P. falciparum parasites. (stanford.edu)
  • BK virus, simian virus 40, and JC virus DNA sequences have been detected in human lymphoid tissues, blood cells, and lymphomas ( 17 - 20 ). (cdc.gov)
  • AN - check the tag INFANT HN - 2008 FX - Child Nutrition FX - Infant Nutrition Physiology FX - Milk FX - Milk, Human DH - Adolescent Nutrition DI - 052508 MN - SP6.021.067 MS - Nutrition of persons 10 through 19 years of age. (bvsalud.org)
  • The genelists, data sheets, and product profiles of the Pig Genome Oligo Set Version 1.0 and Pig_extension Genome Oligo Set Version 1.0 are available from Qiagen . (iastate.edu)
  • FusionSeq: a modular framework for finding gene fusions by analyzing paired-end RNA-sequencing data. (tufts.edu)
  • This gene has no protein-coding homolog in any other genome but its presence is supported by evidence from expression and hybridization data. (ubc.ca)
  • Notice for patent related sequence data 4. (nig.ac.jp)
  • In order to clarify the biological and pharmacological mechanisms of O. sinensis , we carried out the genome sequencing of H. sinensis, anamorph of O. sinensis , for the first time, described the annotation and gene expression, and analyzed the complete sequencing data (Li et al. (springeropen.com)
  • A framework for variation discovery and genotyping using next-generation DNA sequencing data. (nordiag.com)
  • A Better Way to Find RNA Virus Needles in the Proverbial Database Haystacks Researchers combed through more than 5,000 data sets of RNA sequences generated from diverse environmental samples around the world, resulting in a five-fold increase of RNA virus diversity. (doe.gov)
  • An integrated framework for cDNA library production, sequencing, quality control, expression data generation, and systems-level analysis is presented and utilized. (umsystem.edu)
  • These early adopters have taken a leap of faith that having genome sequence data readily at hand to guide health management will eventually yield improved health outcomes. (cdc.gov)
  • The existence of early adopter organizations generating sequence data on large numbers of individuals with paired longitudinal electronic health record data provides opportunities for real-world clinical research directed at health outcome endpoints. (cdc.gov)
  • Time will tell if such collaborations can be developed and maintained, but failure to do so would seem a missed opportunity to generate clinical utility data for using genome sequencing as a screening tool to prevent disease and improve population health. (cdc.gov)
  • This makes it difficult to write programs that can unambiguously determine that two EST libraries were sequenced from the same tissue. (wikipedia.org)
  • In many organisms, such as soybean, the best source of sequence information is the existence of expressed sequence tag (EST) libraries. (biomedcentral.com)
  • Many EST libraries were constructed from RNA extracted from various genetic backgrounds, thus gene identification from these sources is complicated by the existence of both gene and allele sequence differences. (biomedcentral.com)
  • The genome of Aspergillus oryzae , a fungus important for the production of traditional fermented foods and beverages in Japan, has been sequenced. (nature.com)
  • A TIGR sequence annotation tool has been developed at the Iowa State University. (iastate.edu)
  • Anticipated orthologous ORFs in the regions above were defined as BLAT (Kent 2002) or SSearch (Pearson and Lipman 1988) sequence matches. (ubc.ca)
  • Over 300,000 putative SNPs have been identified, of which over 48,000 are high quality SNPs as defined by contig size of at least four sequences and the minor allele presence of at least twice in the contig. (auburn.edu)
  • The expansion in genome size appears to be characteristic of the organisms closely related to A. oryzae , as the estimated genome size of its close relatives A. flavus (W. Nierman, personal communication) and Aspergillus niger 8 is comparable to that of A. oryzae . (nature.com)
  • The flat file includes the sequence and the information of submitters, references, source organisms, and "feature" information, etc. (nig.ac.jp)
  • 2 − 6 These nuclease-resistant polymers persist in live organisms for days and are typically designed to recognize 25-base sequences that span intron-exon junctions or translational start sites. (cdc.gov)
  • Transgenic frogs that express the enzyme in the germline can be bred with animals harboring a transposon substrate to generate double transgenic lines where remobilization will occur in the germline in subsequent generations. (biomedcentral.com)
  • What was also innovative, even though it no longer seems so strange, was placing a set of researchers at the service of a risky undertaking: there was no assurance, as is common in cutting-edge fields, that the FAPESP Genome program would generate significant results. (fapesp.br)
  • But with two projects, communities are forming to generate high quality genomes to benefit researchers. (doe.gov)
  • They may be represented in databases as either cDNA/mRNA sequence or as the reverse complement of the mRNA, the template strand. (wikipedia.org)
  • Positions Contents --------- -------- 01-05 'LOCUS' 06-12 spaces 13-28 Locus name 29-29 space 30-40 Length of sequence, right-justified 41-41 space 42-43 'bp' 44-47 spaces 48-54 DNA, RNA, mRNA, rRNA, tRNA or cRNA, left justified 55-55 space 56-63 'linear' followed by two spaces, or 'circular' 64-64 space 65-67 The division code (see '3.1. (nig.ac.jp)
  • More loci were mapped in the B genome (5774) than in the A (5173) or D (5146) genomes . (bvsalud.org)
  • Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
  • 16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)
  • The article, which described the first complete sequencing of DNA of a plant pathogen, was the work of a São Paulo network of 192 researchers supported by FAPESP. (fapesp.br)
  • Fernando identified the scientific opportunity, sequencing a genome, while Perez identified the ways and means - a virtual institute or network of researchers. (fapesp.br)
  • There were those who argued that the objective of the program (sequencing genomes) was work for computers, not for researchers. (fapesp.br)
  • Genome Insider: Methane Makers in Yosemite's Lakes Meet researchers who sampled the microbial communities living in the mountaintop lakes of the Sierra Nevada mountains to see how climate change affects freshwater ecosystems, and how those ecosystems work. (doe.gov)
  • Microsatellite markers provide a powerful tool in genome researches due to their wide distribution, codominant inheritance and high polymorphism. (biomedcentral.com)
  • Different types of probes may investigate a broad range of sizes of DNA sequence. (msdmanuals.com)
  • DNA from a patient is compared to a reference genome using many oligonucleotide probes. (msdmanuals.com)
  • Non-natural oligonucleotides are valuable probes of biological systems, as they can convey synthetic control of endogenous nucleic acids with high sequence specificity. (cdc.gov)
  • sequences at breakpoint junctions. (tufts.edu)
  • The EST resource should also be valuable for identification of microsatellites, comparative genome analysis. (auburn.edu)
  • Sequence analysis of the Daphnia pulex genome holds some surprises that could not have been anticipated from what was learned so far from other arthropod genomes. (biomedcentral.com)
  • Here we report mapping of 7104 expressed sequence tag (EST) unigenes by Southern hybridization into a chromosome bin map using a set of wheat aneuploids and deletion stocks. (bvsalud.org)
  • Findings of the clonal integration of MCPyV in tumor cell genomes, tumor-associated mutations in the large T-antigen (T-ag) gene, and large T-ag expression in tumors suggest that MCPyV is not only associated with MCC, but that it may be the causative agent ( 1 , 12 , 13 ). (cdc.gov)
  • Congenital chromosome changes and other mutations in the DNA sequence can cause many conditions, for example cancer. (lu.se)
  • Mattias Höglund and his staff have drawn up a classification system for bladder cancer based on information on genetic mutations and changes in gene expression in both the genome and the epigenome. (lu.se)
  • For instance, cancer origin of a library is often mixed with the tissue name (e.g., the tissue name "glioblastoma" indicates that the EST library was sequenced from brain tissue and the disease condition is cancer). (wikipedia.org)
  • They tend to be expressed at low levels and in a tissue-specific manner, and they are enriched for roles in motor activity, cell adhesion, connective tissue, and central nervous system development. (nih.gov)
  • Considerable progress has been achieved in Conifer genome mapping by the use of megagametophyte tissue and RAPDs (3). (le.ac.uk)
  • The blue bars at the bottom indicate the regions syntenic with A. fumigatus ( AF ) and A. nidulans ( AN ) genomes (see Methods). (nature.com)
  • The resulting sequence is a relatively low-quality fragment whose length is limited by current technology to approximately 500 to 800 nucleotides. (wikipedia.org)
  • To gain deeper insight into the biological and pharmacological mechanisms, we sequenced the genome of H. sinensis . (springeropen.com)
  • These fragments are then ligated by two short adaptors, which provide primers for both amplification and sequencing of the fragment as well as a biotin tag that immobilises it onto a streptavidin-coated bead. (outsourcing-pharma.com)
  • Sex determination in cattle based on simultaneous amplification of a new male-specific DNA sequence and an autosomal locus using the same primers. (fbn-dummerstorf.de)
  • Biased phylodynamic inferences from analysing clusters of viral sequences. (cdc.gov)
  • Next-generation sequencing technologies have dramatically changed the approach to genetic diagnosis. (msdmanuals.com)
  • This revolutionary and rapidly evolving technology has moved a significant portion of the technical aspects of genetic diagnosis to next-generation sequencing and has become the mainstay of genetic diagnosis. (msdmanuals.com)
  • Expressed Sequence Tags" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ouhsc.edu)
  • Unexpectedly complex mitoribosomes in Andalucia godoyi , a protist with the most bacteria-like mitochondrial genome. (umontreal.ca)
  • Tips for a Winning Community Science Program Proposal In the Genome Insider podcast, tips to successfully avail of the JGI's proposal calls, many through the Community Science Program. (doe.gov)
  • This suggests that the genome size differences are largely due to sequence acquisition in A. oryzae . (nature.com)