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  • genes
  • First, a genome-wide RNAi screen for C. elegans SMN modifier genes was undertaken, yielding four genes. (nih.gov)
  • SmedGD integrates in a single web-accessible portal all available data associated with the planarian genome, including predicted and annotated genes, ESTs, protein homologies, gene expression patterns and RNAi phenotypes. (nih.gov)
  • In doing so, we uncovered an unexpected diversity of very long and complex DNA sequences repeated throughout the N. brasiliensis genome, including massive tandem repeats of tRNA genes. (beds.ac.uk)
  • genomics
  • In Parasite Genomics Protocols, pioneering bench researchers describe in detail the cutting-edge techniques they have developed for analyzing the genomes and gene products in a diverse range of protozoan and metazoan parasites. (springer.com)
  • Helminth Genomics initiated at the dawn of the millennium with promised to provide novel tools to decipher the biology of several parasitic worms that are agents of some of the most prevalent neglected diseases. (frontiersin.org)
  • The gene predictions were made by the Parasite Genomics group at the Wellcome Trust Sanger Institute and WormBase, as part of the 50 Helminth Genomes project. (wormbase.org)
  • sequence
  • Some of the protocols require DNA sequence data, whereas others were developed independently of whole-genome sequence data. (springer.com)
  • In order to make the extensive data associated with the genome sequence accessible to the biomedical and planarian communities, we have created the Schmidtea mediterranea Genome Database (SmedGD). (nih.gov)
  • The BLAT result page contains a button that will link the results to an auto-generated track in the browser, such that the queried sequence will be aligned visually to the genome and will be in correct alignment with the other data tracks. (nih.gov)
  • We, therefore, took N. brasiliensis as a test case to evaluate the possibility of generating a genome sequence de novo from a heterogeneous population using MinION long reads and improved analysis methods. (beds.ac.uk)
  • sequences
  • Additional chapters survey the available genome sequences, helping novice researchers overcome the difficulties of accessing the databases and applying bioinformatics analysis to biological function. (springer.com)
  • Both nucleotide and protein sequences can be used with BLAST, while BLAT will search the whole genome with nucleotide sequences only. (nih.gov)
  • This is a particularly acute problem for holocentric nematodes because of the large number of satellite DNA sequences found throughout their genomes. (beds.ac.uk)
  • assemblies
  • Indeed, there have now been successful chromosome-scale assemblies of large genomes, primarily using the PacBio SMRT platform to generate contigs (e.g. [ 14 ]), combined with long-range linking information (e.g. [ 15 , 16 ]) but the approach can still be challenging. (beds.ac.uk)
  • immune
  • Helminths evoke immune regulatory pathways often involving dendritic cells, regulatory T cells, and macrophages that help to control disease. (jimmunol.org)
  • Investigators are identifying key helminth-derived immune modulatory molecules that may have therapeutic usefulness in the control of inflammatory disease. (jimmunol.org)
  • Epidemiologic data and animal experimentation suggest that elimination of helminths contributes to the increasing prevalence of some immune-mediated diseases in regions with ever-improving sanitation. (jimmunol.org)
  • Helminths modulate intestinal inflammation through activation of interactive immune regulatory circuits involving regulatory T cells (Tregs), dendritic cells (DCs), macrophages, and several cytokines ( Fig. 1 ). (jimmunol.org)
  • contig
  • The contig and location are hyperlinked to the genome browser for further inspection. (nih.gov)
  • analysis
  • Reference genome availability allowed for variome studies, such as genome-wide analysis based on variable markers associated with different pathologies or drug resistance. (frontiersin.org)
  • studies
  • Studies, mostly in animal models of human disease, are providing insight into how helminths mediate protection from these conditions. (jimmunol.org)
  • successful
  • The advent of site-specific mutagenesis with CRISPR-Cas9 systems boosted hopes for rapid and simple tools to analyze helminth genomes, and the first few successful efforts have recently been reported. (frontiersin.org)
  • life
  • Genomes on their own cannot explain the life cycles of roundworms (nematodes) or flatworms (trematodes and cestodes). (frontiersin.org)