Genome during infection. Medical search. Frequent questions

COVID-19-compatible symptoms, and symptomatic HCP sequencing demonstrated that SARS-CoV-2 viral genomes were sent home per MDH and CDC guidance. (cdc.gov)
Conclusion Since all yet available whole genome sequences of TPA comes from bacteria multiplificated in rabbits, sequencing of syphilis genomic DNA isolated directly from the patient is required. (bmj.com)
Each of the HSV-2 genome sequences was initially obtained using next-generation sequencing and completed with Sanger sequencing. (emmes.com)
We used next-generation sequencing techniques to determine whole-genome sequences of four viral samples from two subjects in the Herpevac Trial. (emmes.com)
CDC used whole genome sequencing (WGS) analysis to link one case to contaminated opened powdered formula from the patient's home and the other to contaminated breast pump equipment. (medscape.com)
They are classified into at least three species, HRV-A, HRV-B and HRV-C, which are characterized by sequencing the 5′ untranslated region (UTR) or the VP4/VP2 region of the genome. (plos.org)
Comparison of Control of Clostridium difficile Infection in Six English Hospitals Using Whole-Genome Sequencing. (ox.ac.uk)
Methods: We used whole-genome sequencing (WGS) of consecutive C. difficile isolates from 6 English hospitals over 1 year (2013-14) to compare infection control performance. (ox.ac.uk)
The virus was sequenced at the Institut Pasteur's Mutualized Platform for Microbiology (P2M), which performs genome sequencing on bacterial, viral, fungal and parasite strains received by National Reference Centers and World Health Organization Collaborating Centers for the purpose of infectious disease surveillance. (pasteur.fr)
Sequencing the genome of pathogens is crucial for the development of specific diagnostic tests and the identification of potential treatment options," explains Sylvie van der Werf, Director of the National Reference Center (CNR) for Respiratory Viruses at the Institut Pasteur. (pasteur.fr)
That same Friday evening, scientists launched the process of sequencing the viral genome based on the samples. (pasteur.fr)
The sequencing run was completed by early evening on the Tuesday, and the scientists used data analysis to obtain the sequence of the whole genome in two of the first three confirmed cases in France. (pasteur.fr)
Whole genome sequencing is the next step in genetic testing for Autism but is not yet widely available in ordinary medical settings. (medindia.net)
In contrast, whole genome sequencing offers sensitive views of the genetic differences between strains ofM. (9lib.org)
tuberculosis strains in whole genome sequencing data. (9lib.org)
Using a combination of field experiments and genome sequencing, the researchers were able to infer the colonisation history of L. japonicus in Japan and identify areas in the genome where plant populations adapted to warm and cold climates , respectively, showed extreme genetic differentiation. (phys.org)
See how the University of Washington used HiFi sequencing to uncover a key finding about ALS and the human genome. (pacb.com)
Whole-genome sequencing was performed using single-molecule real-time (SMRT) technology, and de novo assembly revealed a single contig with 396-fold sequence coverage. (pacb.com)
Our revolutionary sequencing technologies combine the completeness of long reads with the accuracy of short reads to provide the most comprehensive view of genomes, transcriptomes, and epigenomes. (pacb.com)
The minimal sequencing depth of each sample is 100 × of H37Rv genome size. (biomedcentral.com)
Bacterial isolates were subjected to whole-genome sequencing (WGS). (lu.se)
The conclusion from the abstract states simply: "Our pilot study of genome sequencing in a national health care system showed an increase in diagnostic yield across a range of rare diseases. (jax.org)
Not surprisingly, the paper concludes that the findings from the pilot study support the case for using whole genome sequencing for diagnosing rare disease patients. (jax.org)
Sequencing of monkeypox virus from infected patients reveals viral genomes with APOBEC3-like editing, gene inactivation, and bacterial agents of skin superinfection. (bvsalud.org)
The sequencing of the human genome revealed that at least 1% of the human genome consists of endogenous retroviral sequences, representing past encounters with retroviruses during the course of human evolution. (msdmanuals.com)
This manageable size together with the current advances in nucleotide sequencing technology means that partial and whole virus genome sequencing will become an essential component in epidemiologic investigations of disease outbreaks. (msdmanuals.com)

To date, complete genome sequences of 6 TPA strains (all passed through rabbits) have been determined. (bmj.com)
We determined the genome sequences of viruses isolated from two subjects in the Herpevac Trial for Women who experienced primary HSV-2 genital disease and compared them with sequences of viruses isolated from the subsequent fifth or sixth episode of recurrent disease in the same individuals. (emmes.com)
We report here the whole-genome sequences of the human-infective Trichuris trichiura and the mouse laboratory model Trichuris muris . (nature.com)
Here we present high-quality genome sequences for T. trichiura and T. muris , the first duo of a major human STH and its mouse counterpart. (nature.com)
As they reported today in Nature Genetics , the researchers then assembled those non-reference sequences into more than 125,700 "African pan-genome contigs," including hundreds of contigs that overlapped with and altered protein-coding genes. (genomeweb.com)
In a statement, Salzberg further noted that one problem with relying on a single reference genome is that "when a particular DNA analysis doesn't match the reference and you throw away those non-matching sequences, those discarded bits may in fact hold the answers and clues you are seeking. (genomeweb.com)
The team's analysis suggested that an African pan-genome bolstered by these new sequences contained roughly 10 percent more DNA than found in the GRCh38 human reference genome. (genomeweb.com)
It placed the 296.5 million bases of sequences that resisted alignment with the reference genome into 125,715 contigs, including 33,599 contigs present in the genomes of two or more of the CAAPA participants and tens of thousands of contigs that closely resembled sequences in Korean or Chinese genomes. (genomeweb.com)
Each of the CAAPA participants carried an average of 859 African pan-genome contigs, the researchers reported, and these 387 inserted sequences overlapped with known genes, producing 315 distinct protein-coding gene compositions. (genomeweb.com)
And when they looked at the distribution of the African pan-genome sequences in genomes from a dozen more African individuals and a dozen European individuals enrolled in the Simons Genome Diversity Project, the investigators found that while some of the sequences turned up in individuals of European ancestry, they were far more common in the African individuals profiled. (genomeweb.com)
Genome sequences of 358 bacterial cultures have been determined with WGS. (biomedcentral.com)
Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)

To decipher the potential roles of lncRNAs during infection, we identified 365 protein-coding genes that were associated with 214 ISELs. (biomedcentral.com)
The comprehensive use of copy number variant (CNV) genetic testing offers an important tool in individualized diagnosis and treatment of autism, suggest the investigators with the Autism Genome Project, the world's largest research project on identifying genes associated with risk for autism. (medindia.net)
We found that after SARS-CoV-2 infection, this cohesin structure was depleted from the TADs and some genes critical to the interferon response subsequently lost their enhancer-promoter contacts. (springernature.com)
Genome-wide expression of low temperature response genes in Rosa hybrida L.. Plant Physiology and Biochemistry. (usda.gov)
Now, scientists from the USDA Agricultural Research Service (ARS) and the Boyce Thompson Institute (BTI) have created a pan-genome that captures all of the genetic information of 725 cultivated and closely related wild tomatoes, mapping 4,873 genes that were absent from the original reference genome . (isaaa.org)
The first tomato genome sequence was released in 2012, revealing approximately 35,000 genes and facilitating crop improvement efforts. (isaaa.org)
The pan-genome helps identify additional genes that might be available for crop breeding and improvement. (isaaa.org)
The ∼30 Mb genomes of the Plasmodium parasites that cause malaria each encode ∼5000 genes, but the functions of the majority remain unknown. (portlandpress.com)
2021). Genome-Wide DNA Methylation Profiling of Esophageal Squamous Cell Carcinoma from Global High-Incidence Regions Identifies Crucial Genes and Potential Cancer Markers. (who.int)
To identify Escherichia coli genes potentially regulated by filamentous phage infection, we used oligonucleotide microarrays. (lu.se)
The analysis revealed altered transcription levels of 12 E. coli genes in response to phage infection, and the observed regulation of phage genes correlated with the known in vivo pattern of M13 mRNA species. (lu.se)

Insti- genome sequence analyses showed that the participant tutional review board approval was obtained from the Uni- repeatedly experienced infections by the same strain over versity of Iowa. (cdc.gov)
Little genetic drift was detected in viruses shed by the same subjects following repeated reactivation events, suggesting strong selective pressure on the viral genome to maintain sequence fidelity during reactivations from its latent state within an individual host. (emmes.com)
The latest cycle threshold (Ct) values of coronavirus (COVID-19) positive tests, as well as latest analysis of the genetic lineages of COVID-19 seen in the samples we sequence, are provided in our Coronavirus (COVID-19) Infection Survey dataset . (ons.gov.uk)
On January 29, 2020, the Institut Pasteur, which is responsible for monitoring respiratory viruses in France, sequenced the whole genome of the coronavirus known as "2019-nCoV", becoming the first institution in Europe to sequence the virus since the start of the outbreak. (pasteur.fr)
Over the weekend of January 11-12, the Chinese authorities shared the full sequence of the coronavirus genome, as detected in samples taken from the first patients. (pasteur.fr)
NEW YORK (GenomeWeb) - Using genome sequence data from more than 900 individuals of African ancestry, a team led by researchers at Johns Hopkins University has identified hundreds of millions of bases that are not represented in the human reference genome. (genomeweb.com)
The researchers analyzed deep whole-genome sequence data from 910 previously sequenced African individuals, uncovering more than 296 million bases of sequence that did not align to version GRCh38 of the human reference genome. (genomeweb.com)
The GRCh38 reference genome represents more than 3 billion bases of sequence data, the team noted. (genomeweb.com)
Complete genome sequence of Pseudomonas aeruginosa PA1, isolated from a patient with a respiratory tract infection. (pacb.com)
We report the 6,498,072-bp complete genome sequence of Pseudomonas aeruginosa PA1, which was isolated from a patient with a respiratory tract infection in Chongqing, People's Republic of China. (pacb.com)
Complete genome sequence of Enterobacter cloacae subsp. (genome.jp)
The multi-locus sequence type and the number of single nucleotide polymorphisms (SNPs) compared to an index genome were determined. (lu.se)
The paper provides a glimpse of the huge amount of thoughtful work being done by the 100,000 Genomes Project to incorporate genomics into medical practice across the U.K. It has been a many years-long process, and establishing the infrastructure to enroll patients, sequence their genomes, handle the massive amounts of resulting data, and develop efficient, accurate analysis pipelines has been a daunting task. (jax.org)
And while the 100,000-genome goal seemed audacious at the time, the preliminary clinical results have been promising enough to expand the project, which is now working to sequence and analyze millions of patient genomes. (jax.org)
HBV is characterized by a genetic heterogeneity and 8 genotypes (A to H) can be classified based on comparison of complete HBV genomes and according to the criterion of ≥ 8% differences in the complete nucleotide sequence of the viral genome [4-6]. (who.int)
Classification of viruses is principally according to their genome sequence taking into consideration nature and structure of their genome and their method of replication, but not according to the diseases they cause (see International Committee on Taxonomy of Viruses (ICTV), 2021 release ). (msdmanuals.com)
Positive-sense RNA viruses possess a single-stranded RNA genome that can serve as messenger RNA (mRNA) that can be directly translated to produce an amino acid sequence. (msdmanuals.com)

Methods: Patients presenting postpartum with signs and symptoms of infection were cultured for β-haemolytic streptococci with cervical swabs and blood cultures, and bacterial isolates were species-determined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and emm-typed. (lu.se)
Additionally, we evaluated the joint effect of the host and pathogen genomes in association with severity of SAB infection via logistic regression, including an interaction of host SNP with bacterial genotype, and adjusting for age (by decade), sex, the 6 significant principal components, and dialysis status. (biomedcentral.com)
The initiation and severity of S. aureus infections is complex and influenced by at least 3 characteristics: bacterial virulence factors, host genetic factors, and the environment in which the host and pathogen interact. (biomedcentral.com)
This detailed volume presents a diverse set of methodological approaches designed to improve our understanding of bacterial infections from a wide range of bacterial species. (lu.se)
Beginning with biofilms and subcellular compartments, the book explores transcriptional analysis, methods for studying plasmid dynamics, and tools for phylogenetic analysis of bacterial genomes, as well as bacterial effector proteins interfering with host systems, host response analysis, and in vivo and in vitro infection models. (lu.se)

We analyzed the skin microbiome of 153 samples (6 species) from 2017 in order to assess any association between the amphibian skin microbiome and the probability of infection amongst different host species. (nature.com)
From November 2003, when a resurgence of H5N1 virus activity in humans and animals occurred, through September 2017, 1,838 human infections with avian influenza viruses in the region were reported to WHO. (who.int)
2017). Genome-scale mutational signatures of aflatoxin in cells, mice, and human tumors. (who.int)

However, whole-genome se- reus (MSSA), belonging to livestock clade ST398, in a quence analyses with 89 previously published clonal com- farmer in Iowa, USA. (cdc.gov)
In this study, we undertook extensive genome-wide comparative analyses of twelve species that conform the Pectobacterium genus. (mdpi.com)
Regional analyses of human infections with avian influenza subtypes revealed distinct patterns and variations in epidemiology across countries, age, and time. (who.int)
As infected animals and contaminated environments are the primary source of human infections, regional analyses that bring together surveillance data from human and animal health sectors are an important basis for exposure and transmission risk assessment and public health action. (who.int)
2019). Experimental and pan-cancer genome analyses reveal widespread contribution of acrylamide exposure to carcinogenesis in humans. (who.int)

In September 2021, a state public health department reported an infant with C. sakazakii infection to CDC (patient A). In February 2022, a physician reported another case to CDC and a different state health department (patient B). CDC was invited to participate in the investigations and distributed case report forms to obtain detailed feeding information about the two cases. (medscape.com)
Since the end of June 2022, most COVID-19 infections in the UK have been Omicron variant BA.5 or its sub-lineages, more recently the majority of which were the sub-lineage BQ.1. (ons.gov.uk)
A large outbreak of Monkeypox virus (MPXV) infections has arisen in May 2022 in nonendemic countries. (bvsalud.org)

The isolates from this patient and The 61-year-old man enrolling in the Iowa study his wife formed a distinct clade within the ST398 global in July 2011 reported a skin infection on his foot. (cdc.gov)
In a real-world scenario, using a dataset of 50 paired clinical isolates from a study of patients with either reinfections or relapses, we found that QuantTB could detect mixed infections and reinfections at rates concordant with a manually curated approach. (9lib.org)

t571 strains have been rarely isolat- and recurrent infection with methicillin-sensitive S. au- ed from Iowa livestock ( 8 ). (cdc.gov)
Given the increased interest for novel HRV strain identification and their worldwide distribution, we have carried out clinical and molecular diagnosis of HRV strains in a 2-year study of children with acute respiratory infection visiting one district hospital in Shanghai. (plos.org)
Therefore, strains lacking T3SS were thought to be less pathogenic due to the absence of injectosome machinery and its effector toxins (ExoS, ExoT, ExoU, and ExoY) that play a prominent role in acute as well as chronic infections ( Hauser, 2009 ). (frontiersin.org)

Ancient pathogen genomics is an additional approach that can confirm the infection, even when there are no lesions present on skeletal remains or when disease manifestation is atypical. (biomedcentral.com)

Instead, a better approach may be to create reference genomes for all distinct human populations, which over time will eventually yield a comprehensive pan-genome capturing all of the DNA present in humans. (genomeweb.com)
Scale up WGS of foodborne pathogens to better understand foodborne resistance patterns and outbreaks, and find new ways to prevent foodborne infections in humans. (cdc.gov)
Humans vary in their susceptibility to acquiring Staphylococcus aureus infection, and research suggests that there is a genetic basis for this variability. (biomedcentral.com)
Since the first confirmed human infection with avian influenza A(H5N1) virus was reported in Hong Kong Special Administrative Region in 1997, sporadic zoonotic avian influenza viruses causing illness in humans have been identified globally, with the WHO Western Pacific Region as one of the hotspots. (who.int)
An overview of the epidemiology and emergence of influenza A infection in humans over time. (who.int)

The M. leprae strain clustered in branch 3 together with the oldest to-date genome from the UK. (biomedcentral.com)
We sequenced and constructed the complete genome of an environmental strain CR1 of P. aeruginosa and performed the comparative genomic analysis. (frontiersin.org)
We identified eight new regions of genomic plasticity and a plasmid pCR1 with a VirB/D4 complex followed by trimeric auto-transporter that can induce virulence phenotype in the genome of strain CR1. (frontiersin.org)
To date, WHO has designated 4 SARS CoV-2 genotypes that meet the working definition of a VOC: i) contain mutations divergent from the Wuhan strain and ii) that the mutations impact one or more of the aspects of viral infection that have public health significance. (who.int)

Double or triple infections with HRV-C and respiratory syncytial virus and/or bocavirus were diagnosed in 33.3% of the HRV-infected patients, but no correlation with severity of clinical outcome was observed. (plos.org)
The Metabolism, Infection and Immunity (MINI) Section at the National Institutes of Health (NIH) aims to define the relationship between infection, immunity and clinical decline in individuals with mitochondrial disease. (genome.gov)
Infection in individuals with mitochondrial disease can lead to worsening clinical symptoms, particularly neurologic symptoms. (genome.gov)
The 100,000 Genomes Project used HPO to generate standardized baseline clinical data, guided by disease-specific data models. (jax.org)
The study also developed an automated diagnostic pipeline to streamline the genomic data- including the millions of variants present in each genome-for clinical interpretation. (jax.org)
Dengue virus infection has emerged as marker in clinical practice ( 11 ). (who.int)

In parallel, multiple technologies and manual finishing were used to produce an 85-Mb reference genome assembly from the more readily available mouse parasite species T. muris (Online Methods and Supplementary Note ). (nature.com)
Overall, these results suggest that a single reference genome is not adequate for population-based studies of human genetics," senior and co-corresponding author Steven Salzberg, a computational biology, biostatistics, and biomedical engineering researcher at Johns Hopkins, and his colleagues wrote. (genomeweb.com)
Even so, the work to accurately represent a broader range of human populations in the human reference genome is not finished. (genomeweb.com)
Despite these efforts, the current human reference genome derives primarily from a single individual, thus limiting its usefulness for genetic studies, especially among admixed populations, such as those representing the African diaspora," the authors wrote, noting that the "lack of diversity in the reference genome poses many challenges when analyzing individuals whose genetic background does not match the reference. (genomeweb.com)
and we detected 35 mutations relatively to genome ON563414.3 (a B.1 lineage reference genome ). (bvsalud.org)

The full time series of our official estimates from 27 April 2020 onwards are available in our Coronavirus (COVID-19) Infection Survey dataset . (ons.gov.uk)

Polymorphisms over the entire genomes were mapped, and amino acid variants resulting from nonsynonymous changes were analyzed based on the secondary and tertiary structures of a previously crystallized protein. (emmes.com)
Several recent genome-wide association studies (GWAS) have identified variants that may affect susceptibility to infectious diseases, demonstrating the potential value of GWAS in this arena. (biomedcentral.com)
Ours is the first study that has attempted to evaluate the entire human genome for variants potentially involved in the acquisition or severity of SAB. (biomedcentral.com)
Future investigations using larger samples, narrowed phenotypes, and advances in both genotyping and analytical methodologies will be important tools for identifying causative variants for this common and serious cause of healthcare-associated infection. (biomedcentral.com)

All HBV genomes are of genotype D4 which is rare in Europe today. (biomedcentral.com)
Genome-wide CRISPR-Cas9 knockout screens aim to elucidate the relationship between genotype and phenotype by ablating gene expression on a genome-wide scale and studying the resulting phenotypic alterations. (wikipedia.org)

Although, the genetic basis of adaptation to parasite infection has been widely studied, little is known about how epigenetic changes contribute to parasite resistance and eventually, adaptation. (uni-kiel.de)
Only 19 genetic polymorphisms unique to the primary or recurrent isolate were identified, 10 in subject A and 9 in subject B. These observations indicate remarkable genetic conservation between primary and recurrent episodes of HSV-2 infection and imply that strong selection pressures exist to maintain the fidelity of the viral genome during repeated reactivations from its latent state. (emmes.com)
These technologies allow genetic material to be added, removed, or altered at particular locations in the genome. (medlineplus.gov)
Overall, the 100,000 Genomes team was able to make genetic diagnoses in 25% of the patients. (jax.org)
Here, we review the arrival of genetic screens in malaria parasites to analyse parasite gene function at a genome-scale and their impact on understanding parasite biology. (portlandpress.com)
We therefore introduce the reader to CRISPR-based screens in the related apicomplexan Toxoplasma gondii and discuss how these approaches could be adapted to develop CRISPR/Cas9 based genome-scale genetic screens in malaria parasites. (portlandpress.com)

Classification of the MPXV genomes and determination of their mutational patterns were performed using Nextclade. (bvsalud.org)

Silver Age of GOLD Introduces New Features The Genomes OnLine Database makes curated microbiome metadata that follows community standards freely available and enables large-scale comparative genomics analysis initiatives. (doe.gov)
Host life-history traits, skin microbiome composition and phylogeny were associated with lower prevalence of infection for high altitude species. (nature.com)
Herein, we performed a deep shotgun metagenomics study with the aim to obtain a more precise landscape of gut microbiome dysbiosis in HIV-1 infection. (frontiersin.org)
These findings advance our understanding of human gut microbiome and their potential associations with HIV-1 infection. (frontiersin.org)
Characteristics of the early child gut microbiome may influence the course of enteric infections, and enteric infections may change the composition of the gut microbiome, all of which may have long-term implications for child growth and development. (bmj.com)
Methods and analysis We are conducting a community-based birth cohort study to examine interactions between gut microbiome conditions and enteric infections, and how environmental conditions affect the development of the gut microbiome. (bmj.com)
From 6 to 12 months of age, we will collect weekly stool samples to compare microbiome conditions in diarrhoea stools versus stools from healthy children prior to, during and after acute enteric infections, using principal-coordinate analysis and other multivariate statistical methods. (bmj.com)

Herpes simplex virus 1 (HSV-1) and HSV-2 are large, double-stranded DNA viruses that cause lifelong persistent infections characterized by periods of quiescence and recurrent disease. (emmes.com)
The vast majority of infections were with H7N9 (n=1,562) and H5N1 (n=238) viruses, and most (n=1583, 86%) were reported from December through April. (who.int)
Negative-sense RNA viruses possess a single-stranded negative-sense genome that first must synthesize a complementary positive-sense antigenome, which is then used to make genomic negative-sense RNA. (msdmanuals.com)
the genome of RNA viruses ranges from 3.5 kilobases (some retroviruses) to 27 kilobases (some reoviruses), and the genome of DNA viruses ranges from 5 kilobases (some parvoviruses) to 280 kilobases (some poxviruses). (msdmanuals.com)

All 18 genomes were classified in clade IIb, lineage B.1, and we identified four B.1 sublineages (B.1.1, B.1.10, B.1.12, B.1.14). (bvsalud.org)

We detected a high number of mutations (range, 64-73) relatively to a 2018 Nigerian genome ( genome GenBank Accession no. (bvsalud.org)
2018). Genome-wide profiling of normal gastric mucosa identifies Helicobacter pylori- and cancer-associated DNA methylome changes. (who.int)

Whole genome amplification (WGA) was carried out by multiple displacement amplification (MDA) with phi 29 polymerase after specific separation of TPA on the cell level from the human cells. (bmj.com)
Here, we present molecular evidence of infection with four different pathogens (human parvovirus B19 (B19), HBV, variola virus, and M. leprae ) in 22 individuals. (biomedcentral.com)
Human rhinoviruses (HRVs) are a highly prevalent cause of acute respiratory infection in children. (plos.org)
It has been successfully applied to edit human genomes, and has started to displace RNAi as the dominant tool in mammalian studies. (wikipedia.org)
The availability of these two important whipworm genomes and the integration of parasite and host data presented here will underpin future efforts to control these parasites and exploit their immunological interplay for human benefit. (nature.com)
And after more than a decade of ongoing improvements to it, gaps in the human genome have been shrinking, while efforts such as the Genome Reference Consortium have been attempting to flesh out forms of genomic variation with alternate loci. (genomeweb.com)
Genome editing is of great interest in the prevention and treatment of human diseases. (medlineplus.gov)
It also holds promise for the treatment and prevention of more complex diseases , such as cancer, heart disease, mental illness, and human immunodeficiency virus (HIV) infection. (medlineplus.gov)
Ethical concerns arise when genome editing, using technologies such as CRISPR-Cas9, is used to alter human genomes. (medlineplus.gov)
Germline cell and embryo genome editing bring up a number of ethical challenges, including whether it would be permissible to use this technology to enhance normal human traits (such as height or intelligence). (medlineplus.gov)
Human Germline Genome Editing. (medlineplus.gov)
Human papillomavirus infection of epithelium. (medscape.com)

The production of RNAi libraries facilitated the application of this technology on a genome-wide scale, and RNAi-based methods became the predominant approach for genome-wide knockdown screens. (wikipedia.org)
The CRISPR-Cas9 system has generated a lot of excitement in the scientific community because it is faster, cheaper, more accurate, and more efficient than other genome editing methods. (medlineplus.gov)

Institut de Recherche pour le Développement (IRD), Microbes Evolution Phylogeny and Infections (MEPHI), Aix-Marseille Univ. (bvsalud.org)

Sixty-four HRV-infected outpatients were diagnosed amongst 827 children with acute low respiratory tract infection. (plos.org)
Since completion of this work, which focuses on what occurs immediately upon acute infection, the ever-evolving conversation surrounding 'long-COVID' or PASC (post-acute sequelae of COVID-19) has inspired us to consider the long-term effects of chromatin alterations-especially those that interfere with the delicate balance of the immune system. (springernature.com)
Further investigation into these ideas is certainly required, however, we believe changes in this environment could represent a possible link between what happens during acute infection and the persistent symptoms we can now see in cases of long-COVID. (springernature.com)
Doxycycline is the treatment of choice for acute Q fever, and 2 weeks of treatment is recommended for adults, children aged 8 years or older, and for severe infections in patients of any age. (medscape.com)
Ph.D. student, I hope to contribute to the knowledge in the field of HIV-1 plasma proteomics during acute infection and also characterize key drivers of HIV transmission and spread in Subsaharan Africa. (lu.se)

The researchers brought together nearly 1.2 trillion paired-end reads for the analysis - the 910 individuals included in the study had their genomes sequenced to an average depth of 30- to 40-fold by the Consortium on Asthma among African-Ancestry Populations in the Americas (CAAPA). (genomeweb.com)
Overall, we observed low prevalence and burden of infection in host populations, suggesting that Bd is enzootic in Taiwan where it causes subclinical infections. (nature.com)

The VOC differ from the original Wuhan virus as they have mutations across the genome. (who.int)
Mutations in other regions of the genome may also be informative for known VOI/VOC detection. (who.int)
Overall, these assays and approaches detect specific mutations or features such as insertions, deletions and point mutations in the SARS CoV-2 genome that are characteristic of a particular VOC/VOI using PCR. (who.int)

The genome atlases allowed for distinguishing distinct DNA structures and highlighted suspicious high transcription zones. (mdpi.com)

Genome Insider: Methane Makers in Yosemite's Lakes Meet researchers who sampled the microbial communities living in the mountaintop lakes of the Sierra Nevada mountains to see how climate change affects freshwater ecosystems, and how those ecosystems work. (doe.gov)
But with two projects, communities are forming to generate high quality genomes to benefit researchers. (doe.gov)

2023). Impact of HPV vaccination on HPV-related oral infections. (who.int)

Experimental parasite infection causes genome-wide changes in DNA methylation. (uni-kiel.de)
Here, we investigated the role of host DNA methylation modifications to respond to parasite infections. (uni-kiel.de)
In a controlled infection experiment, we used the three-spined stickleback fish, a model species for host-parasite studies, and their nematode parasite Camallanus lacustris. (uni-kiel.de)
Altogether, we demonstrate that parasite infection contributes to genome-wide DNA methylation modifications. (uni-kiel.de)
Together, these genomes and associated functional data elucidate key aspects of the molecular host-parasite interactions that define chronic whipworm infection. (nature.com)

Over 30% of the individuals exhibit molecular signs of infection with hepatitis B virus (HBV), parvovirus B19, variola virus (VARV), and Mycobacterium leprae . (biomedcentral.com)
Molecular Evolution of Herpes Simplex Virus 2 Complete Genomes: Comparison between Primary and Recurrent Infections. (emmes.com)
Detection of mixed infections has been limited to molecular genotyping techniques, which lack the sensitivity and resolution to accurately estimate the multiplicity of TB infections. (9lib.org)

Gene expression data from mice with low-dose whipworm infection provide a detailed description of a regulated T H 1-like immune response to the infected cecum that is not limited to the immediate site of infection. (nature.com)

Translational Genomics Research Institute, infection culture received in November 2011 (Table). (cdc.gov)
The Global Emerging Infections Surveillance and Response System has been a critical partner for the VDB as a sponsor of our SARS-CoV-2 surveillance efforts over the course of the pandemic," said Dr. Matthew Conte, lead of the VDB Viral Genomics and Bioinformatics section. (health.mil)

We produced a 75.2-Mb high-quality draft genome assembly from a clinically isolated adult male T. trichiura using Illumina technology. (nature.com)
A first draft genome of the Sugarcane borer, Diatraea saccharalis. (usda.gov)

citation needed] Nevertheless, RNAi-based approaches to genome-wide knockdown screens have their limitations. (wikipedia.org)
Several approaches to genome editing have been developed. (medlineplus.gov)
So this meeting will examine emerging data relating to macrophage interactions with the immune system during HIV infection, macrophage reservoirs and approaches to their elimination, and the involvement of CNS myeloid reservoirs and associated comorbidities. (nih.gov)

In recent years technical breakthroughs have made forward and reverse genome-scale screens in Plasmodium possible. (portlandpress.com)

A new gene known as mcr -1-which can make bacteria resistant to colistin, a last-resort drug for some multidrug-resistant infections-was first reported in China in November 2015 and in the United States in May 2016. (cdc.gov)
In November 2015, mcr -1-a gene that can make bacteria resistant to colistin, an old antibiotic that is the last-resort drug for some multidrug-resistant infections-was reported in China. (cdc.gov)
Colistin has recently been used, and increasingly, to treat patients with infections caused by multidrug-resistant bacteria against which colistin is still effective, despite its side effects. (cdc.gov)
If colistin resistance spreads to bacteria that are already resistant to all other antibiotics, those bacteria could cause truly untreatable infections. (cdc.gov)
CRISPR-Cas9 was adapted from a naturally occurring genome editing system that bacteria use as an immune defense. (medlineplus.gov)

2016). DNA Methylation in Newborns and Maternal Smoking in Pregnancy: Genome-wide Consortium Meta-analysis. (who.int)

The genome is condensed, non segmented and consists of a single molecule of circular, supercoiled double-stranded DNA, 12000 base pairs in length. (wikidoc.org)

The extent to which the HSV-2 genome evolves during multiple episodes of reactivation from its latent state within an infected individual is not known. (emmes.com)
After initial infection of the viral genome the virus may become latent within the host. (wikidoc.org)

Although resistance to oxacillin (MIC 4 µg/mL by broth dilution), colonization with ST398 is common in livestock workers, in- making this a borderline-resistant S. aureus infection. (cdc.gov)
Finally, there is considerable variability within the population of patients who develop S. aureus infection, with some patients recovering and others developing a range of complications, including death. (biomedcentral.com)

At each level of genome organization that we explored, we found that the observed changes were more severe in the SARS-CoV-2 infected condition than in any of our other viral infections or immune stimulants. (springernature.com)

However, ongoing surveillance of the infection is warranted, as changing environmental conditions may disturb the currently stable equilibrium. (nature.com)

Host species, geography (elevation), climatic conditions and microbial richness were all associated with the prevalence of infection. (nature.com)

E proteins made by the viral genome promote the activation of host DNA replication mechanisms that can then be used by the virus during its own replication. (medscape.com)

However, only few studies have profiled fungal lncRNAs in a genome-wide manner during host infection. (biomedcentral.com)

[ 12 ] Fortunately, 90% of infections with HPV are thought to clear within 2 years due to a cell-mediated immune response. (medscape.com)
She has longstanding research experience in infection and immune function in people with neurodevelopmental disorders, including those with mitochondrial diseases. (genome.gov)
So on day one, Session 1 will focus on macrophages and the immune system during HIV and SARS-CoV-2 infection, Session 2 will focus on challenges to study HIV/SIV reservoirs, and Session 3 will really have the highlights from recent awardees of the NIMH/NINDS/NIDA Myeloid Reservoir RFA. (nih.gov)

it then relies on the differentiation of the keratinocytes to reach the surface and be shed for continued infection. (medscape.com)
Conclusion: QuantTB can determine infection multiplicity, identify hetero-resistance patterns, enable differentiation between relapse and re-infection, and clarify transmission events across seemingly unrelated patients- even in low-coverage (1×) samples. (9lib.org)
Patterns of genome-wide variation, population differentiation and SNP discovery of the red banded stink bug (Piezodorus guildinii). (usda.gov)

Almost all precancerous and cancer lesions are associated with long-term, persistent HPV infection. (medscape.com)

Retroviruses use reverse transcription to create a double-stranded DNA copy (a provirus) of their RNA genome, which is inserted into the genome of their host cell. (msdmanuals.com)

Thus, global transcriptional analysis and functional analysis revealed previously unknown host responses to filamentous phage infection. (lu.se)

Background: Mixed infections ofMycobacterium tuberculosis and antibiotic heteroresistance continue to complicate tuberculosis (TB) diagnosis and treatment. (9lib.org)
Expand state programs to better respond to outbreaks, improve antibiotic prescribing, and prevent antibiotic-resistant infections across all healthcare settings. (cdc.gov)

Anatomy15

Organisms33

Diseases3

Chemicals and Drugs38

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes142

Disciplines and Occupations5

Technology, Industry, Agriculture3

Information Science15

Health Care5

Sequencing26

Sequences12

Genes11

Sequence17

Bacterial5

20173

Analyses5

20223

Isolates2

Strains3

Pathogen1

Humans5

Strain4

Clinical6

Reference genome5

20201

Variants4

Genotype2

Genetic6

Classification1

Microbiome7

Viruses4

Lineage1

20182

Human12

Methods2

Phylogeny1

Acute5

Populations2

Mutations3

Distinct1

Researchers2

20231

Parasite5

Molecular3

Mice1

Genomics2

Draft2

Approaches3

Plasmodium1

Bacteria5

Methylation1

Single-molecule1

Latent2

Aureus infection2

Viral infections1

Surveillance1

Prevalence of infection1

Replication1

Fungal1

Immune3

Differentiation3

Persistent1

Double-stranded1

Analysis1

Antibiotic2