• We were building YAC [yeast artificial chromosome] libraries, cosmid libraries. (nih.gov)
  • Sequencing of six neighbouring EcoRI fragments of cosmid UKG041 from chromosome XI. (elsevierpure.com)
  • In his study, he carries out multidisciplinary Cosmid and Chromosome research. (research.com)
  • To facilitate M. grisea genome analysis, physical mapping, and positional cloning, we have constructed a bacterial artificial chromosome (BAC) library from the rice infecting strain 70-15. (johnshopkins.edu)
  • The sequencing of the zebra finch Taeniopygia guttata genome, in combination with targeted bacterial artificial chromosome (BAC) sequencing, has allowed us to characterize an MHC from a highly divergent and diverse avian lineage, the passerines. (biomedcentral.com)
  • FBN3 (D6S24): a bovine microsatellite derived from cosmid cIOBT475 at chromosome 6q12-14. (fbn-dummerstorf.de)
  • Eggen A., Solinas T.S., Fries R.A., Cosmid specific for sequences encoding a microtubule associated protein, MAPIB, contains a polymorphic microsatellite and maps to bovine chromosome 20q14, J. Hered. (gse-journal.org)
  • The genome of A. xylosoxidans consists of a 7-Mb chromosome and two large plasmids (98 kb and 248 kb). (inrae.fr)
  • Its genome consists of a 3.7-Mb chromosome and a 133-kb plasmid. (inrae.fr)
  • Transposons: These are DNA sequences that can move and replicate in different parts of a cell's genome. (wikipedia.org)
  • Reintroduction of cloned DNA sequences into the genome has recently been accomplished. (cshlpress.com)
  • Highly repetitive regions are unstable in plasmid/cosmids/BACs so their length and sequences couldn't be accurately determined. (blogspot.com)
  • The analyzed X. bovienii draft genomes are broadly similar in structure (e.g. size, GC content, number of coding sequences). (biomedcentral.com)
  • This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones. (nih.gov)
  • Ploidy and MYCN amplification have been used as genetic markers for risk stratification and therapeutic decision making, and, more recently, gene expression profiling and genome-wide DNA copy number analysis have come into the picture as sensitive and specific tools for assessing prognosis. (biomedcentral.com)
  • The applica tion of new genetic tools also led to the discovery of an important familial neuroblastoma cancer gene, ALK , which is mutated in approximately 8% of sporadic tumors, and genome-wide association studies have unveiled loci with risk alleles for neuroblastoma development. (biomedcentral.com)
  • Treatment of human immunodeficiency virus (HIV) infection with ZFN-mediated CCR5 gene disruption is one of the indicator examples of the ability of ZFNs in genome editing. (ac.ir)
  • when the first major genome update was published in 2004, the estimated gene count was revised to 24,000 [ 8 ]. (biomedcentral.com)
  • Thus, the increase in genome size seems to be due to an A. oryzae lineage-specific acquisition of sequence, rather than loss of sequence in A. nidulans and A. fumigatus . (nature.com)
  • This suggests that the genome size differences are largely due to sequence acquisition in A. oryzae . (nature.com)
  • Sandwalk: The 20th anniversary of the human genome sequence: 2. (blogspot.com)
  • It's been 20 years since the first drafts of the human genome sequence were published. (blogspot.com)
  • The IHGP sequence covered about 82% of the genome and it contained about 250,000 gaps and millions of sequencing errors. (blogspot.com)
  • International Human Genome Sequencing Consortium (2004) Finishing the euchromatic sequence of the human genome. (blogspot.com)
  • The amount of actual nucleotide sequence in the latest version of the reference genome ( GRCh38.p13 ) is 3,110,748,599 bp and the estimated total size is 3,272,116,950 bp based on estimating the size of the remaining gaps. (blogspot.com)
  • It's now possible to complete the human genome reference sequence by sequencing at least one individual but I'm not sure that the effort and the expense are worth it. (blogspot.com)
  • So even if we could perfectly sequence two genomes, their total lengths would not be the same. (blogspot.com)
  • A pink broken line denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron). (nih.gov)
  • The report urges DOE and the Nation to commit to a large, multi-year, multidisciplinary, technological undertaking to order and sequence the human genome. (ornl.gov)
  • The zebra finch MHC differs markedly from that of the chicken, the only other bird species with a complete genome sequence. (biomedcentral.com)
  • Introduction to genomic & Proteomics, Structural genomics and proteomics, Human Genome project and other sequencing projects, Biological databases and data mining, sequence similarity search and sequence alignment, Protein structure predication and structure analysis, use of software package in Bioinformatics. (bits-pilani.ac.in)
  • Here, we report the complete genome sequence. (inrae.fr)
  • Sequencing of the A. oryzae genome was accomplished using the whole-genome shotgun (WGS) approach. (nature.com)
  • Phylogenetic analysis of the three aspergilli using the whole-genome data showed that A. nidulans branched off earlier than A. oryzae and A. fumigatus 5 . (nature.com)
  • Twenty years ago genetic studies were based not on disease but on chromosomes, because it was so hard to study a whole genome. (nih.gov)
  • 2006) Bovine Whole Genome Assembly release Btau_3.1. (nist.gov)
  • To discern strain-level variation among symbiosis factors, and facilitate the identification of novel compounds, we performed a comparative analysis of the genomes of 10 Xenorhabdus bovienii bacterial strains. (biomedcentral.com)
  • Genome content analysis revealed that general classes of putative host-microbe interaction functions, such as secretion systems and toxin classes, were identified in all bacterial strains. (biomedcentral.com)
  • Targeted genome editing using CRISPR-Cas9 has been widely adopted as a genetic engineering tool in various biological systems. (mdpi.com)
  • The emergence of TALENs in 2010 has enabled the genome modification of non- model organisms, while the emergence of the CRISPR/Cas9 system in 2013 as a revolutionary genome-editing tool has allowed us to anticipate the forthcoming new era of genome editing research. (ac.ir)
  • The 16S rDNA is present in scattered form in the entire genome of bacteria. (ispub.com)
  • It alone represents 9% of the yeast genome (8% if ribosomal DNA is included). (inrae.fr)
  • Mobile genetic elements (MGEs) sometimes called selfish genetic elements are a type of genetic material that can move around within a genome, or that can be transferred from one species or replicon to another. (wikipedia.org)
  • Comparison of the three aspergilli species revealed the presence of syntenic blocks and A. oryzae -specific blocks (lacking synteny with A. nidulans and A. fumigatus ) in a mosaic manner throughout the genome of A. oryzae . (nature.com)
  • Additional alginate loci were located within the P. aeruginosa PAO1 genome utilizing isogenic mucoid mutants coupled with PAO1-derived cosmid complementation. (marshall.edu)
  • Targeted genome engineering or editing enables researchers to modify genomic loci of interest in a precise manner, which has a turning point in medicine, biological research, and biotechnology. (ac.ir)
  • The genome was confirmed to comprise eight chromosomes (chromosomes 1-8 in decreasing size), the assignment of which is different from a previous report 7 ( Supplementary Table S1 and schematic drawing in Supplementary Fig. S1 ). (nature.com)
  • Cytogenetic (FISH) evidence and the genome assembly itself place core MHC genes on as many as four chromosomes with TAP and Class I genes mapping to different chromosomes. (biomedcentral.com)
  • Therefore, free chromatin mapping can be generally used to study the structure and organization of mammalian genomes. (nih.gov)
  • The blue bars at the bottom indicate the regions syntenic with A. fumigatus ( AF ) and A. nidulans ( AN ) genomes (see Methods). (nature.com)
  • One hundred cosmid clones, each with ≈40-Kb DNA insert, were partially sequenced in both directions by using primers M13 and T7. (cdc.gov)
  • Genome-wide association studies (GWAS) attempt to link genetic variants to nearby genes, relying on standard catalogs of human genes. (biomedcentral.com)
  • The expansion in genome size appears to be characteristic of the organisms closely related to A. oryzae , as the estimated genome size of its close relatives A. flavus (W. Nierman, personal communication) and Aspergillus niger 8 is comparable to that of A. oryzae . (nature.com)
  • Genome editing using targetable nucleases is an emerging technology for precise genome modification in many organisms with hight ability and capability. (ac.ir)
  • The result of todays genome initiatives promise huge, immense scientific and agricultural advantages and aspect to a brand new predictive method of the behavior of destiny examine in biology. (joecolgan.com)
  • Journal of Genome follows a progressive editorial policy that encourages researchers to submit the original research, reviews and editorial observations as articles, well supported by tables and graphic representation. (omicsonline.org)
  • It facilitated communication among genome researchers and informed persons interested in genome research. (ornl.gov)
  • Physical mapping of the human genome by pulsed field gel analysis. (joecolgan.com)
  • Physical mapping of complex genomes by cosmid multiplex analysis. (joecolgan.com)
  • Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. (umn.edu)
  • DOE JGI in Walnut Creek, California, provides state-of-the-science capabilities for genome sequencing and analysis. (ornl.gov)
  • These data show that the BAC library is suitable for genome analysis of M. grisea. (johnshopkins.edu)
  • This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. (umn.edu)
  • All targeted genome engineering relies on the introduction of a site-specific double-strand break (DSB) in a pre-determined genomic locus by a rare-cutting DNA endonuclease . (ac.ir)
  • Genomic mutation identifier (COSV) to indicate the definitive position of the variant on the genome. (sanger.ac.uk)
  • analyses gradually elucidated the perfected genome-scale metabolic map of C. glutamicum , expanding the scope of its application. (frontiersin.org)
  • Biocomputing: Informatics and Genome Projects represents a survey of the wishes and ambitions of genome initiatives as of the early Nineties. (joecolgan.com)
  • These first drafts from the International Human Genome Project (IHGP) and Celera were far from complete. (blogspot.com)
  • We've known for many decades that the correct size of the human genome is close to 3,200,000 kb or 3.2 Gb. (blogspot.com)
  • Morton, N.E. (1991) Parameters of the human genome. (blogspot.com)
  • Dulbecco, R. (1986) A turning point in cancer research: Sequencing the human genome. (joecolgan.com)
  • On behalf of the Health and Environmental Research Advisory Committee (HERAC), I am pleased to submit to you the enclosed Report on the Human Genome Initiative. (ornl.gov)
  • The actual ordering and sequencing involves the coordinated processing of some 3 billion bases from a reference human genome. (ornl.gov)
  • During the Human Genome Project, most efforts to estimate and annotate genes focused on protein-coding genes, i.e., regions of the genome that are transcribed into RNA and then translated into proteins. (biomedcentral.com)
  • The 37-Mb genome was predicted to contain a total of 12,074 genes encoding proteins with a length greater than 100 amino acid residues (see Methods). (nature.com)
  • Unrelated phenotypic differences in biofilm formation and lung colonization as well as variations in genome size, all within different sources of P. aeruginosa were discovered and could lead to variations in morbidity and mortality related to P. aeruginosa infections in CF. A key factor in alginate regulation is AlgU/AlgT, a sigma factor that also impacts the expression of many other involved proteins. (marshall.edu)
  • A cosmid library of the genome of C. jejuni 81116 was constructed and probed with Campylobacter hyoilei genes involved in LPS synthesis. (unipr.it)
  • 6 ). A cosmid DNA library was constructed by using pWEB kit (Epicenter Biotechnologies, Madison, WI, USA) by following protocols described elsewhere ( 7 ). (cdc.gov)
  • TALENs: A widely applicable technology for targeted genome editing. (ac.ir)
  • Here we show that the 37-megabase (Mb) genome of A. oryzae contains 12,074 genes and is expanded by 7-9 Mb in comparison with the genomes of Aspergillus nidulans 5 and Aspergillus fumigatus 6 . (nature.com)
  • This study provides the first genome-wide captures of the dynamics of in vivo AmtR binding events and the regulatory network they define. (frontiersin.org)
  • It is used in combination with the shuttle vectors pZAP2.1 or pZAP2.2 to construct replication-deficient adenoviruses containing transgenes in place of the E1 region of the Ad5 genome. (od260.com)
  • Due to its high polymorphism and importance for disease resistance, the major histocompatibility complex (MHC) has been an important focus of many vertebrate genome projects. (biomedcentral.com)
  • In this article, we review essential genome editing tools, summarize their applications in crop improvement, as well as, next-generation crop breeding and their computational resources will be discussed. (ac.ir)
  • Journal of Genome brings articles in all areas related to Chronic diseases on trimonthly basis. (omicsonline.org)
  • In his articles, he combines various disciplines, including Restriction enzyme and Cosmid. (research.com)
  • Journal of Genome welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. (omicsonline.org)
  • In humans, approximately 50% of the genome is thought to be MGEs. (wikipedia.org)
  • The A. oryzae genome is larger than those of A. fumigatus and A. nidulans by approximately 34% and 29%, respectively. (nature.com)