A previous study showed that pretreatment of mouse oocytes with 3% (0.09 M) sucrose allowed visualization of the metaphase spindle and chromosomes under standard light microscopy and led to a 100% enucleation rate. (ox.ac.uk)
Staining of DNA with Hoechst 33342 revealed that these projections coincided with the position of the metaphasechromosomes in 100% of sucrose-treated oocytes, whereas only 31% of oocytes showed alignment of the position of Pb1 with their nuclear materials. (ox.ac.uk)
This study demonstrated that 0.3 M sucrose treatment of bovine oocytes facilitates the localization of metaphasechromosomes under normal light microscopy and hence increases enucleation efficiency without compromising the in vitro development potential of cloned embryos by nuclear transfer. (ox.ac.uk)
It gives our clients all tools to improve collected metaphases images quality, cut unnecessary parts of the image, separate chromosomes with multiple separation methods. (imicrotec.com)
Chromosomes are normally visible under a light microscope only during the metaphase of cell division (where all chromosomes are aligned in the center of the cell in their condensed form). (wikipedia.org)
Common chromosomal fragile sites (CFSs) are genomic regions prone to form breaks and gaps on metaphasechromosomes during conditions of replication stress. (ku.dk)
Chromosomes are studied by cytogenetic techniques and are best studied at mitotic or meiotic metaphase, although some studies, such as fluorescence in situ hybridization (FISH) methods, may utilize interphase cells. (clinicalsci.info)
and fluorescent in, situ hybridization (FISH) to metaphasechromosomes revealed an insertion of part of chromosome 16 on chromosome 11. (lu.se)
In summary, we present several lines of evidence that regional differences in condensation between homologs are programmed during metaphase chromosome compaction. (cytognomix.com)
Construction of a Bacterial Artificial Chromosome Library of TM-1, a Standard Line for Genetics and Genomics in Upland Cotton [J]. J Integr Plant Biol. (jipb.net)
After detection of the sSMC T in metaphase directed fluorescence in situ hybridization (FISH), sex-chromosome-specific microsatellite analysis and interphase-FISH were additionally applied. (lidsen.com)
During metaphase the X-shaped structure is called a metaphase chromosome, which is highly condensed and thus easiest to distinguish and study. (wikipedia.org)
It can be used to confirm or rule out small chromosome deletions, and to characterize subtle, complex chromosome rearrangements in interphase or metaphase cells. (geneticscenter.com)
When the oocyte is in the metaphase II stage of cell division, the spindle-chromosome complex is removed and inserted into a healthy donor oocyte from which the nucleus has already been removed. (msdmanuals.com)
1. Defining a mechanism by which the key mitotic kinase, Aurora B orchestrates safe passage through the metaphase-anaphase transition in the presence of mitotic catenation. (mun.ca)
Roumina Rouhani Ardeshiri, MSc Candidate Dr. Leitges' Laboratory "The Aurora B specificity switch is required to protect from nondisjunction at the metaphase/anaphase transition" Objectives: 1. (mun.ca)
Mitotic metaphase1
Chromosomal ultrastructure and ribonucleoproteins (RNP) at mitotic metaphase in garlic (Allium sativum L. ) were studied. (jipb.net)
Prophase1
Primary spermatocytes are the male germ cells before meiosis I. To examine whether these 4n diploid cells are genetically competent to fertilize oocytes and support full embryo development, we introduced the nuclei of pachytene/diplotene spermatocytes into oocytes that were arrested in prophase I (germinal vesicle stage), metaphase I, or metaphase II (Met II). (elsevierpure.com)
Genes1
Human genetics is the study of the human genome and the transmission of genes from one generation to the next. (amboss.com)
Oocytes3
Hypertonic medium treatment for localization of nuclear material in bovine metaphase II oocytes. (ox.ac.uk)
Oocytes enucleated at the second metaphase stage (MII) are often used as recipient cytoplasts for nuclear transfer. (ox.ac.uk)
After activation of the Met II oocytes that were produced, those microfertilized at metaphase I showed the best developmental ability in vitro, and three of these embryos developed into full-term offspring after embryo transfer. (elsevierpure.com)
It is here suggested that the word "cytogenomics" should rather be used with the goal to paraphrase a new field of research in genomics and diagnostics in human genetics, with an integrative and comprehensive view. (frontiersin.org)
Metaphase karyotypes of Anopheles (Nyssorhynchus) darlingi and Anopheles (N.) nuneztovari from Manaus and Highway BR-174 (Manaus-Boa Vista), State of Amazonas, and Macapa, State of Amapa, Brazil, showed 2n = 6. (ibict.br)
Homologous1
This study describes differences in metaphase compaction between homologous euchromatic loci. (cytognomix.com)
Epigenetics1
OBM Genetics is an international Open Access journal published quarterly online by LIDSEN Publishing Inc. It accepts papers addressing basic and medical aspects of genetics and epigenetics and also ethical, legal and social issues. (lidsen.com)
Metaphase is the third of the five phases of biological cell division, or more specifically, the division of what is inside that cell's nucleus. (sciencing.com)
Spindle1
It is presumed to stop the metaphase stage therefore preventing microtubule formation in the mitotic spindle. (medscape.com)
Arrest1
The vinca alkaloid agents are naturally occurring or semisynthetic nitrogenous bases that elicit their cytotoxic effects through tubulin-binding properties and disruption of microtubule function, which lead to metaphase arrest. (medscape.com)
Advances2
Dame Anne McLaren, English geneticist who pioneered fundamental advances in mammalian genetics and embryology that. (britannica.com)
Genetic diversity has traditionally been accessed from pedigree, however, with the advances in molecular genetics new opportunities have emerged. (scirp.org)
Syndromes1
Currently Genetics Center offers FISH analysis for the following microdeletion syndromes: Angelman syndrome, Prader-Willi syndrome, DiGeorge sequence, Velocardiofacial syndrome, Williams syndrome, Miller-Dieker syndrome and Smith-Magenis syndrome. (geneticscenter.com)
19581
Joshua Lederberg, American geneticist, pioneer in the field of bacterial genetics, who shared the 1958 Nobel Prize. (britannica.com)
Laboratory2
2 . Present, evaluate and interpret qualitative and quantitative laboratory data, in order to develop lines of argument and make sound judgements in accordance with basic theories of chemistry and genetics. (academiasupport.co.uk)
The Genetics Center FISH laboratory is also equipped to test for many types of leukemia. (geneticscenter.com)
Protein1
Overview of Genetics A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)
Since the beginning of the 21st century and the first publication of the full human genome, our knowledge around genomes and molecular genetics has been dramatically improved by the introduction of large-scale high-throughput technologies. (mdpi.com)
Accepts1
Genetics Center accepts both routine and more challenging specimens. (geneticscenter.com)
Study2
Genetics, an essential part of the study of evolution, looks at the inheritance of characteristics by children from their parents. (britannica.com)
The goal of this study was to investigate the impact of multiple exposomal factors (genetics, lifestyle factors, environmental/occupational exposures) on pulmonary inflammation and corresponding alterations in local/systemic immune parameters. (cdc.gov)
Basic1
1. Demonstrate knowledge and understanding of basic ideas and concepts in chemistry and molecular genetics. (academiasupport.co.uk)