Loading...
  • undergoes
  • The team manipulated a stage in the cell cycle called quiescence, when the cell undergoes a period of supposed hibernation and ceases to develop. (asu.edu)
  • In response to a variety of stimuli, including RTK-mediated signals, the mammalian heart undergoes morphological changes that contribute to the development of dilated or hypertrophic cardiomyopathies ( Heineke and Molkentin, 2006 ). (biologists.org)
  • embryo
  • Willadsen coated the embryo with an agar jelly made from algae to reduce the damage caused by entry of the glass needle into the cell membrane. (asu.edu)
  • transgenic strain serve as a source of RFP tagged tissue for the derivation of cell lines and explant, transplant and embryo chimera experiments. (biomedcentral.com)
  • Adult amphibians, though, have been successfully cloned for many years from embryo nuclei. (encyclopedia.com)
  • In this technique, nuclei from cells of an early embryo are extracted using a very fine glass pipette and placed in egg cells that have been shed by a female amphibian such as a frog (after removing the unfertilized egg cell nucleus). (encyclopedia.com)
  • tractable
  • Progress would be greatly facilitated by the availability of a genetically tractable system that recapitulates the features of age-associated decline in the brain under a rapid lifespan. (thermofisher.com)
  • The mouse Mus musculus has had an impact on the lives of human beings since the dawn of civilization, from being an agricultural pest to serving as a domesticated pet, and now contributing a more utilitarian role as the foremost genetically tractable model organism for understanding human biology and disease. (biologists.org)
  • More so than any other genetically tractable organism, the mouse offers a close glimpse of humankind in terms of similarity in the underlying physiology, tissue structure and organization. (biologists.org)
  • transgenes
  • To facilitate the analysis of the mushroom body we provide a compilation of transgenic fruit flies that express transgenes under direct control of the Kenyon-cell specific promoter, mb247. (frontiersin.org)
  • The transgenes expressed are fluorescence reporters to analyze neuroanatomical aspects of the mushroom body, proteins to restrict ectopic gene expression to mushroom bodies, or fluorescent sensors to monitor physiological parameters of neuronal activity of Kenyon cells. (frontiersin.org)
  • mammal
  • Dolly , female Finn Dorset sheep that lived from 1996 to 2003, the first clone of an adult mammal , produced by British developmental biologist Ian Wilmut and colleagues of the Roslin Institute , near Edinburgh , Scotland . (britannica.com)
  • Dolly was the first mammal created using the nucleus from a cell of a mature adult mammal. (encyclopedia.com)
  • nuclear
  • In 1996, Keith Campbell, Jim McWhir, William Ritchie, and Ian Wilmut at the Roslin Institute in Edinburgh, UK, used nuclear transfer techniques to clone a sheep that was born and gre into an adult. (asu.edu)
  • Ontogenetic establishment of order-specific nuclear organization in the mammalian thalamus. (ohsu.edu)
  • molecules
  • We study myocardial (cardiomyogenesis) and microvascular (angio-arteriognesis) regeneration through gene therapy (using regular and minicircle plasmids as vectors) or adult stem cells genetically modified to overexpress defined molecules. (favaloro.edu.ar)
  • These systems include production of several varieties of soluble immunosuppressive molecules in the uterus and the placenta and strict regulation of the molecules expressed on or by placental trophoblast cells. (wiley.com)
  • The major graft rejection-promoting molecules, human leukocyte antigens (HLAs), are tightly regulated in these cells, with none of HLA-A, HLA-B, or HLA class II antigens expressed. (wiley.com)
  • Trogocytosis is a cell-contact dependent intercellular transfer of membrane fragments and associated molecules. (biomedsearch.com)
  • glial
  • Figure 2C shows data from an experiment in which adult fly brains expressing GFP in glial cells were dissected, dissociated into single cells, stained with Vybrant™ DyeCycle™ Violet Stain, and analyzed on the Attune™ Acoustic Focusing Cytometer (with blue/violet lasers) in high-sensitivity mode. (thermofisher.com)
  • We have generated an animal model with homozygous deletion of the succinate dehydrogenase subunit D gene restricted to cells of glial fibrillary acidic protein lineage (hGFAP‐SDHD mouse). (embopress.org)
  • organs
  • Balla, K. M., Luallen, R. J., Bakowski, M. A. & Troemel, E. R. Cell-to-cell spread of microsporidia causes Caenorhabditis elegans organs to form syncytia. (nature.com)
  • genetic
  • Our laboratory is developing genetic tools and fluorescence assays for manipulating cell cycle re-entry in the adult fly brain ( Figure 1 ) and monitoring subsequent effects on DNA ploidy and cell death. (thermofisher.com)
  • The carotid body contains glia‐like adult stem cells, which, as for brain NSCs, are resistant to genetic mitochondrial damage. (embopress.org)
  • This new milestone will have a great impact on our understanding of mammalian biology, and could significantly change the future of medical diagnosis and therapeutic development, where databases can be queried in silico for potential drug targets or underlying genetic causes of illnesses. (biologists.org)
  • characterization
  • However, neither investigations of activated RTK signaling pathways nor the characterization of hypertrophic cardiomyopathy in the adult fly heart has been previously described. (biologists.org)
  • milestone
  • The announcement in February 1997 of Dolly's birth marked a milestone in science, dispelling decades of presumption that adult mammals could not be cloned and igniting a debate concerning the many possible uses and misuses of mammalian cloning technology. (britannica.com)
  • fusing the nucleus
  • Dolly the sheep was successfully cloned in 1996 by fusing the nucleus from a mammary-gland cell of a Finn Dorset ewe into an enucleated egg cell taken from a Scottish Blackface ewe. (britannica.com)
  • nucleus
  • To enucleate the eggs, Briggs and King used a small glass needle to puncture the cell membrane, enter the cytoplasm, and suck out the nucleus of the egg cell. (asu.edu)
  • Wilmut and his team of researchers at Roslin created her by using electrical pulses to fuse the mammary cell with an unfertilized egg cell, the nucleus of which had been removed. (britannica.com)
  • The fusion process resulted in the transfer of the mammary cell nucleus into the egg cell, which then began to divide. (britannica.com)
  • development
  • Sensory input upregulated the development of adult-born PGNs only in enriched glomeruli. (jneurosci.org)
  • Our data provide evidence for an activity-based mechanism that enhances synaptogenesis of adult-born PGNs during their initial phases of development. (jneurosci.org)
  • Of particular interest were changes that occurred to DNA during an animal's development , whereby patterns in gene expression were altered as cells became increasingly specialized in function. (britannica.com)
  • Prior to this feat, it had been thought that normal mammalian development caused irreversible changes in some portion of the DNA that prevented it from acting as embryonic DNA does. (encyclopedia.com)
  • Reprogramming refers to erasure and remodeling of epigenetic factors during mammalian development or in cell culture. (pbrc.edu)
  • extracellular matrix
  • Extracellular matrix remodeling after proatherosclerotic injury involves an increase in hyaluronan (HA) that is coupled with vascular smooth muscle cell (SMC) migration, proliferation, and with neointima formation. (biomedsearch.com)
  • stably
  • We conclude that an anion sensitive yellow fluorescent protein is optimal for primary screening and that automated electrophysiology of cells stably expressing GlyRs is useful for confirming hits and quantitating the actions of identified compounds. (frontiersin.org)
  • astrocytes
  • Finding from the new study, published recently in Cell Reports (" Leucine Zipper-Bearing Kinase Is a Critical Regulator of Astrocyte Reactivity in the Adult Mammalian CNS "), shows that turning on a gene inside astrocytes results in a smaller scar and, potentially, more effective recovery from injury. (genengnews.com)
  • Genetically modified astrocytes (red) line the lesion border in the injured spinal cord. (genengnews.com)
  • A new mouse study shows that triggering a gene inside astrocytes activates the star-shaped cells and may improve the brain's ability to heal from a range of debilitating conditions, from stroke to concussions and spine injuries. (genengnews.com)
  • We've known that astrocytes can help the brain and spinal cord recover from injury, but we didn't fully understand the trigger that activates these cells," explained co-senior study investigator Mark Goldberg, M.D., chairman of neurology and neurotherapeutics at UT Southwestern. (genengnews.com)
  • populations
  • The Attune™ Acoustic Focusing Cytometer allows us to measure rare events and identify small populations of cells in the fly brain, on the scale of a single brain. (thermofisher.com)
  • Pubmed ID: 11867644 In this paper we analyze survival data of populations of sterilized nematodes, Caenorhabditis elegans, exposed to heat shocks of different duration at the beginning of their adult lives. (jove.com)
  • Progress
  • Another barrier to progress in this field has been the difficulty in obtaining aged adult brain samples and monitoring cell cycle re-entry under normal physiological conditions. (thermofisher.com)
  • The newly arrived neutrophils cause rapid division of these skin cells, which may cause them to progress to melanoma. (embo.org)
  • clinical
  • The findings from previous clinical studies on GH treatment in GH-deficient adults, collectively indicate that the majority of adults with long-standing GH deficiency, whether dating from childhood or acquired in adult life, are compromised both physically and psychologically and can derive clinical benefit from GH replacement. (clinicaltrials.gov)
  • The first 2 reviews in this series have described the defining properties of stem cells, their possible sources, and some initial attempts at their clinical use for tissue regeneration and repair. (biomedsearch.com)