Genetic tissue cytokinin. Medical search. Frequent questions

Zinnia cell culture is a unique model system, which at the administration of certain ratio of cytokinins and auxins can be induced to trans-differentiate into vessel elements. (europa.eu)
This is one of the critical stages of embryogenesis, when an immature embryo becomes independent of some physiological factors, in particular, hormones (mainly auxins, cytokinins, and ABA) of a maternal organism. (springer.com)
Subsequently, we have discussed the crosstalk among them, followed by crosstalk with growth promoting hormones (gibberellins, auxins and cytokinins). (beds.ac.uk)

This new information alters normal cell functions, directing the overproduction of plant hormones (auxin and cytokinin), which ultimately leads to uncontrolled plant cell division, growth and gall development. (msu.edu)
Over the past 15 years, research in the Helariutta lab and elsewhere has uncovered a central role for the phytohormones auxin and cytokinin in vascular patterning. (cam.ac.uk)
Stem cell maintenance in the shoot is under the control of the CLAVATA-WUSCHEL (CLV-WUS) negative feedback-loop, which is tightly integrated with hormone function, in particular auxin and cytokinin that promote cell differentiation and proliferation, respectively 2 . (nature.com)

Species of this genus are known to produce hormones that stimulate plant growth, like cytokinin ( 5 ), and antibiotic peptides as well as different ( 6 ) hydrolyzing enzymes, which are responsible for antagonistic behavior against many plant pathogens. (asm.org)
Factors Affecting Plant Tissue Culture: Growth Media Minerals, Growth factors, Carbon source, Hormones Environmental Factors Light, Temperature, Photoperiod, Sterile condition and Relative Humidity Explant Source Usually, the younger, less differentiated the explant, the better for tissue culture Genetics Different species show differences in amenability to tissue culture. (pdfcoffee.com)
Skoog and Miller (1957) observed that the growth and morphogenesis were controlled by hormones, auxin (root formation), and cytokinin (shoot formation) in tissue culture. (cbsetuts.com)
Transferring into sterile environment is vital for undisturbed cell-process and when influenced by hormones, genetic disposition of a given plant is used for its broad propagation. (labflytrap.com)
Here with the help of combination of hormones (cytokinin and auxin) occurs fibrillation of new stems in large. (labflytrap.com)

The subsequent accumulation of cell division and enlargement in this bundle of tissue gives rise to a new structure known as the root primordium. (wikipedia.org)
One of the main objectives is to discover new genetic engineering targets for enhancing lipid accumulation in plant vegetative tissues, particularly those that have synergistic effects with previously known genes, pathways or regulatory circuits. (bnl.gov)
Xu C, Shanklin J (2016) Triacylglycerol Metabolism, Function, and Accumulation in Plant Vegetative Tissues. (bnl.gov)

The experiment aimed to compare the effects of BA and mT on the regeneration from explants of Daphne mezereum , on the levels of several chemical components, the catalase activity, and to assess genetic diversity of microcuttings. (mdpi.com)
The results from this study suggest that in order to obtain endophyte-free callus tissue, vine explants are to be taken from plants grown in a growth chamber simulating springtime conditions, sterilized in a combination of alcohol, bleach, and chlorine dioxide, and plated on selection media containing an antifungal agent, such as chlorothalonil. (scirp.org)
Induction of new shoot meristems from cultured root explants is a widely used, but poorly understood, process in which apical plant tissues are regenerated from adult somatic tissue through the de novo formation of shoot meristems. (biologists.com)
Sharifi (1995) used hypocotyl and cotyledon explants in Cuminum cyminum tissue culture. (scialert.net)
2003) used embryo explants for cumin tissue culture yielding a large number of shoots within short period of time without any sub culturing. (scialert.net)
Further, MS media supplemented with 0.1-0.01 ppm KIN (kinetin-type of cytokinin) and 1.0 ppm 2,4-D is suitable to produce callus cultures using these explants. (plantcelltechnology.com)
For genetic improvement, information on either some qualification or ability to assist in the breeding process is required, such as the ability to culture seed, explants or other tissues in a sterile laboratory condition. (asianjab.com)
In vitro regeneration from node and leaf explants of Jatropha curcas L. and evaluation of genetic fidelity through RAPD markers. (forda-mof.org)

Benzyladenine (BA) is the most widely used cytokinin in the micropropagation industry due to its effectiveness and affordability. (cas.cz)

Both genetic and physiological studies point to the importance of Auxin in the LR initiation and primordium development in the LR formation process, but cytokinin negatively regulates the growth of the LR. (wikipedia.org)
Dr. Xu's research group uses a combination of molecular genetic, cell biological, biochemical and physiological approaches to dissect the regulatory network governing lipid metaoblism, homoestasis and storage. (bnl.gov)

Although colchicine induces polyploidy and enhances genetic diversity, the concentration of colchicine is crucial for the success of ploidy induction. (scholarsresearchlibrary.com)
In this review, we provide an up-to-date overview of the current knowledge of how A. rhizogenes induces root formation, on the new uses for A. rhizogenes in tissue culture and composite plant production (wild-type shoots with transgenic roots), and the recent development of a disarmed version of A. rhizogenes for stable transgenic plant production. (bioone.org)

The group studies a population of coupled genetic oscillators in the vertebrate embryo termed the segmentation clock. (ens-lyon.fr)
Embryoid is a small, well-organised struc-ture comparable to the sexual embryo, which is produced in tissue culture of dividing embryo genic potential somatic cells. (exama2z.in)
Certain tissues off young invitro grown plantlets like hypocotyl, nucellus and embryo sac. (exama2z.in)
Autonomy is a special structural and functional state of an embryo, reflecting its ability for self-regulation and independence from the adjacent tissues. (springer.com)

The advantages also include production of one cell type isolated from complexity of leaf tissues, accessibility for chemical manipulations and microscope observations, high potential and synchrony of TE differentiation, preservation of vessel element patterning in similarity to zinnia hypocotyls in vivo. (europa.eu)
1. Leaf petiole (0.5-1 cm) or root segments from seven-day old seedlings (1 cm) or cam-bium tissue (0.5 cm3) from storage root can be used as explant. (exama2z.in)
Leaf petiole and root segment can be obtained from aseptically grown seedlings (Cambium tissue can be obtained from surface sterilized stor-age tap root 2. (exama2z.in)
The results of analysis of variance showed that the interaction effect of nitrogen × cytokinin on plant height, number of branches, leaf, stem and whole plant dry weights, essential oil percentage and yield was significant (p ≤0.01). (ac.ir)

The embryos arise from a single cell and has no vascular connection with the maternal callus tissue on the exaplant. (exama2z.in)
It can be isolated aseptically from the bulk of maternal tissues of ovule, seed, or capsule and cultured in vitro under aseptic and controlled physical conditions to grow directly into plantlets. (cbsetuts.com)

Explant tissue was chosen from healthy, actively growing plants grown in a growth room and in a vineyard. (scirp.org)
The plant part which is used for the tissue culture is called as explant. (pdfcoffee.com)
The explant may be of single isolated cells or tissues or any plant organ. (pdfcoffee.com)
Direct embryogenesis - The embryos initiate directly from the explant tissue in the absence of callus proliferation. (exama2z.in)
In this medium the explant will produce sufficient callus tissue. (exama2z.in)

In vitro grown Aloe polyphylla (an endangered medicinal and ornamental aloe) were cultured on full strength Murashige and Skoog basal medium with different concentrations of cytokinins and solidified with 1% Bacteriological Agar (Oxoid No. 1). (cas.cz)
Factors included nitrogen levels (0, 50 and 100 kg N/ha) and cytokinin concentrations (0, 100, 200 and 400 μM). (ac.ir)

Specifically, the Ti plasmid of Agrobacterium tumefaciens is an important tool for plant genetic engineering. (istudy.pk)

Genetic enhancement and diversity can be improved with biotechnological approaches using mutagens, and colchicine is the most widely used in inducing polyploidy levels in many garlic varieties [ 8 , 9 ]. (scholarsresearchlibrary.com)
2015). Furthermore, the genetic diversity of local ecotypes can be maximized if plants are multiplied from seeds (Benson et al. (researchsquare.com)
One understudied opportunity is using feral crops-plants that have escaped and persisted without cultivation-as a source of genetic diversity, which could build resilience in domesticated conspecifics. (weizmann.ac.il)
Some researchers focus on the negative consequences of domestication, whereas others assert that feral populations may serve as useful pools of genetic diversity for future crop improvement. (weizmann.ac.il)

Sophisticated lab facilities are needed Plants cells, tissues and organs grown in artificial medium Optimum environmental conditions are provided Aseptic condition is maintained in tissue culture laboratory Plants are multiplied by asexual method/vegetative methods Progenies obtained are true to type because of asexual reproduction 5. (pdfcoffee.com)
The technique of maintaining and growing cells, tissues, or organs, especially on artificial medium in suitable containers under controlled environmental conditions is known as tissue culture. (cbsetuts.com)
It is typical for all cells , tissues and organs of a plant. (assignmentsbag.com)

Cytokinin acts to promote procambial proliferation at the expense of xylem, while auxin signalling is critical for the specification of the xylem tissues. (cam.ac.uk)

Another challenge to using transgenic plant technology in citrus is the long juvenile phase of immature tissues that ranges from 5 to 20 years, which greatly delays analysis of the effects of candidate genes. (springer.com)
In plants, polytene chromosomes have been observed in only a few species, and seemed to be restricted to ovary and immature seed tissues, e.g., in Phaseolus coccineus and P. vulgaris (Nagl, 1981), until relatively recently, when they were observed in the cells of the anther tapetum of Vigna unguiculata (Guerra and Carvalheira, 1994) and of some Phaseolus species (Carvalheira and Guerra, 1994). (scielo.br)

Xylem vessel dimensions differ with genetic background and are influenced by environmental conditions during the growth. (europa.eu)

Although plant tissue culture has been a successful method to propagate many species, V. aestivalis has not yet been reliably grown as pure callus culture due to a fungal endophyte that exists within the plant. (scirp.org)
Characteristics of plant tissue culture techniques 1. (pdfcoffee.com)
The details of the plant tissue culture technique and its utilization are emphasized here as follows. (cbsetuts.com)
The Components of plant tissue culture media II : Organic additions, osmotic and pH effects, and support systems. (forda-mof.org)
Introduction of Plant Tissue Culture. (ac.ir)

Treatment improved the genetic potential of garlic varieties in vitro but resulted in a lower duplication rate at 0.75 mg/L due to higher toxicity. (scholarsresearchlibrary.com)

Hypersensitivity to cytokinin causes stem cell overproliferation and major rearrangements of Bif3 inflorescence meristems, leading to the formation of ball-shaped ears and severely affecting productivity. (nature.com)

The tissue culture technique is also called as invitro techniques because of the involvement of laboratory in the culture of plant. (pdfcoffee.com)

The effects of a maize knotted1 ( kn1) gene under the control of the cauliflower mosaic virus 35S promoter on genetic transformation efficiencies of six citrus genotypes were tested. (springer.com)
With all six genotypes of citrus tested including Eureka lemon, a cultivar difficult to be transformed, our results demonstrate that the kn1 gene may provide an effective molecular tool to enhance genetic transformation efficiencies of various citrus varieties. (springer.com)
An efficient genetic transformation protocol is a key step for using transgenic technologies to improve citrus quality, productivity, and resistance to abiotic and biotic stresses such as HLB. (springer.com)
On the other hand, transgenic plants produced from mature tissue via genetic transformation can drastically shorten flowering and fruiting time and thus speed up field evaluation studies. (springer.com)
Genetic transformation specifically refers to the techniques that are employed to obtain organisms that have been modified genetically, called the GMOs (genetically modified organisms). (biomedcentral.com)
1989). Genetic transformation may enable the development of transgenic plants with enhanced resistance to wilt diseases. (scialert.net)
1994). Availability of a tissue culture protocol with such criteria would be an important step towards the establishment of a genetic transformation system in this species. (scialert.net)

This system drives the rhythmic, sequential, and precise formation of embryonic body segments, exhibiting rich spatial and temporal phenomena spanning from molecular to tissue scales. (ens-lyon.fr)

Using a genetic screen, we discovered a mutation in a callose synthase gene which led to excess callose deposition at plasmodesmata, disrupting intercellular connectivity and resulting in defects in vascular patterning. (cam.ac.uk)
Genetic testing of family members is done when the index case has a known mutation. (msdmanuals.com)
Family members also require ongoing clinical monitoring for development of arrhythmias unless the genetic mutation is absent. (msdmanuals.com)

Acclimatization of tissue cultured plantlets: From laboratory to land. (forda-mof.org)

of callus tissue to a 250 ml of Erlenmeyer flask containing 20-25 ml of liquid medium of the same composition as used for callus growth (without agar). (exama2z.in)

It, however, has disadvantages such as genetic alteration and abnormal growth in some plants. (cas.cz)
The vascular network provides plants with structural support and long-distance transport, meaning that vascular tissues must predictably differentiate to form continuous strands. (cam.ac.uk)
This technique could potentially be used with plants that have associated endophytes or other contamination problems to establish callus tissue for research and/or commercial propagation efforts. (scirp.org)
Asexual reproduction/propagation (mitosis) is the basis for the tissue culture derived plants. (pdfcoffee.com)
transgenic plants can be produced with the availability of standardised tissue culture technique 8. (pdfcoffee.com)
Maintenance and multiplication of heterozygous plants( cross pollinated plant species ) is easy in tissue culture 10. (pdfcoffee.com)
Plants produced through tissue culture have uniform flowering and maturity 14. (pdfcoffee.com)
Regeneration of a patterned multicellular organism from isolated pieces of adult somatic tissues is a remarkable phenomenon that occurs both in plants and animals ( Morgan, 1901 ). (biologists.com)
Genetic instability of seeds will be reflected in the characteristics of plants. (plantcelltechnology.com)
The selection and screening are important in the case of propagation of plants for industrial or research purposes to maintain genetic uniformity amongst clones. (plantcelltechnology.com)
The technique provides genetic stability to the cultured plants. (plantcelltechnology.com)
So far, most successful genetic engineering of plants has relied on inserting one or a few genes that supply simple, yet useful, properties. (istudy.pk)

The grapevine cells in the injured tissue release chemical compounds (sugars and phenolics) that promote cell growth to repair the wound. (msu.edu)
I will describe recent progress in understanding the behavior of individual cells from the zebrafish as they slow their oscillations and differentiate during segmentation, and discuss how this gives rise to the tissue-level wave patterns. (ens-lyon.fr)
Such cells are found in embryonic tissues. (exama2z.in)
Scientists have exploited this genetic transfer in order to get genes with desired properties into plant cells. (istudy.pk)
With the discovery of the polytenics in tapetum tissue, it was observed that in many other species of various angiosperm families the tapetal cells also display polytene, polyploid or both types of nuclei. (scielo.br)

UNIT-1 Laboratory requirements for tissue culture-culture media and their constituents-cell culture types and applications of cell culture-cell and organ differentiation-somoclonal variation tissue culture technology in India-protoplast cultureregeneration. (pdfcoffee.com)

Genetic engineering provides one such possibility (bringing genes from unrelated species) whereby the focus is on improving various traits that lead to high yields, tolerance to salinity, drought, pests, weeds, and sustainable agricultural traits such as higher water use efficiency (WUE). (biomedcentral.com)
With increasing the amount of nitrogen and cytokinin, all the studied traits (except harvest index) increased significantly compared to the control. (ac.ir)

1994). Potential genetic variability for conventional breeding is limited in Cuminum cyminum (Hunault et al . (scialert.net)
Autoimmune antibody production and inflammatory cytokinins may also be responsible for temporal variability in arrhythmia propensities, such as the deleterious effects of fever in patients with Brugada syndrome. (msdmanuals.com)

The seed collected from ethnic farmers (Pa-gha-ker-yor People), at Pala U village, Hau Hin district, Prachuap Khiri Khan province, Thailand for genetic conservation and investigating feasibility for breeding and improvement. (asianjab.com)
These questions, which emerged from the colloquium "Darwins' reversals: What we now know about Feralization and Crop Wild Relatives" at the BOTANY 2021 conference, fall into seven categories that span both basic and applied research: (1) definitions and drivers of ferality, (2) genetic architecture and pathway, (3) evolutionary history and biogeography, (4) agronomy and breeding, (5) fundamental and applied ecology, (6) collecting and conservation, and (7) taxonomy and best practices. (weizmann.ac.il)

INTRODUCTION TO TISSUE CULTURE Definition: Culture of plant cell, tissue and organ on artificial medium under aseptic condition is called tissue culture. (pdfcoffee.com)

The use of meta-topolin and its derivatives as alternatives to BA and zeatin, both of which frequently have negative effects in tissue culture was investigated. (cas.cz)
This study reports a viable protocol for obtaining fungus-free tissue culture callus from V. aestivalis. (scirp.org)
Tissue culture is based on the concept of totipotency i.e. each cell has the ability to develop in to whole organism. (pdfcoffee.com)
Haberlandt is called as the father of tissue culture. (pdfcoffee.com)
The tissue culture industry has boomed over the past few years because of its extensive benefits and potential roles in research studies, horticulture, and drug industries. (plantcelltechnology.com)
In this article, you will learn about the strategies used for Cannabis sativa propagation and the advantages and disadvantages of using tissue culture for Cannabis propagation. (plantcelltechnology.com)
Embryoid development in tissue culture passes through three stages, namely, globular stage, heart-shaped stage and torpedo stage (Fig. 20). (exama2z.in)
The tissue culture technique was first practiced by Haberlandt (1902). (cbsetuts.com)
In India, work in tissue culture started at the Department of Botany of Delhi University in 1960. (cbsetuts.com)
The shoot and root culture are generally controlled by auxin-cytokinin balance, usually an excess of auxin promotes root culture, whereas that of cytokinin promotes shoot culture. (cbsetuts.com)
In Plant Propagation by Tissue Culture (Vol. 1, pp. 115-174). (forda-mof.org)
2014). Plant propagation by tissue culture. (forda-mof.org)

However, efforts have been made to genetically transform mature citrus tissues but the efficiencies are generally extremely low (Marutani-Hert et al. (springer.com)

Overexpression of ZmWUS1-B is due to multimerized binding sites for type-B RESPONSE REGULATORs (RRs), key transcription factors in cytokinin signaling. (nature.com)

The biochemical and genetic stability makes it a more efficient technique than the suspension cultures. (plantcelltechnology.com)

This pattern of growth gives rise to a bundle of tissue. (wikipedia.org)

Summary: The view that lifespan of an organism is intrinsically limited and is largely species-specific necessarily involves certain notions of genetic elements of regulation. (bio.net)

The medium generally contains auxin like 2, 4-D, and often a Cytokinin like BAP (Benzyl-aminopurine). (cbsetuts.com)

Additional evidence suggests the bacterium distributes unevenly both within and between vines and can survive epiphytically (meaning on the surface) on any kind of vine tissue, including leaves and shoot tips. (msu.edu)

Ontogenetic changes in the photosynthetic apparatus and effects of cytokinins 36: 527-528. (ac.ir)

We use genetic screens in sensitized backgrounds to identify novel components of these networks, as well as other genetic factors regulating vascular development. (cam.ac.uk)

In order to investigate the effect of nitrogen and cytokinin on the quantitative and qualitative characteristics of thyme, a three-year factorial experiment was conducted at Razi University based on a randomized complete block design with 3 replications. (ac.ir)

Once the bacterium is in close contact with a susceptible plant cell, it delivers a piece of its genetic material (known as transfer-DNA) to the grapevine. (msu.edu)
The degree of totipotency is maximum in undifferentiated cell tissues. (pdfcoffee.com)

The shoot auxin transport system involves diverse transporters and mounting evidence suggests that different transporters are differentially sensitive to shoot branching regulatory signals, including auxin flux, cytokinin and strigolactone. (ens-lyon.fr)

Polytene chromosomes are structures found in highly specialized tissues in some animal and plant species, which are amplified through successive cycles of endoreduplication, finally producing several copies of each chromosome. (scielo.br)

Anatomy24

Organisms30

Chemicals and Drugs37

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes28

Technology, Industry, Agriculture7

Information Science4

Auxins3

Auxin and cytokinin3

Hormones5

Accumulation3

Explants8

Micropropagation1

Physiological2

Induces2

Embryo4

Leaf4

Maternal2

Explant5

Concentrations2

Agrobacterium1

Diversity4

Organs3

Proliferation1

Immature2

Xylem1

Plant tissue5

Vitro1

Meristems1

Invitro1

Transformation7

Molecular1

Mutation3

Plantlets1

Callus growth1

Plants12

Cells5

Laboratory1

Traits2

Variability2

Conservation2

Artificial medium1

Culture12

Genetically1

Transcription1

Biochemical1

Growth1

Organism1

Generally1

Vine1

Effects1

Components1

Characteristics1

Cell2

Shoot1

Structures1