Genes transgenic suicide. Medical search. Frequent questions

By crossing AD model mice onto a CD45-deficient background, the researchers created a transgenic strain that fails to clear Aβ oligomers, overproduces the microglial proinflammatory cytokines tumor necrosis factor (TNF)-α and interleukin (IL)-1β, and loses cortical neurons. (alzforum.org)
Younger CD45-deficient transgenic mice and PSAPP mice with normal CD45 did not show these abnormalities. (alzforum.org)
The health and lifespan of mice have been demonstrated to improve by the removal of senescent cells (and reducing the SASP) using a transgenic suicide gene [3], and additional experiments showed that the same could be achieved using small molecules. (lifespan.io)
Advances in the genetic manipulation of mice, modeling various aspects of the AD pathology, have provided an excellent tool to dissect the effects of genes, environment, and their interactions. (ox.ac.uk)
Knock in mice models have more precise portrayal of the disease`s genotype as scientists are able to replace specific gene regions of the HD gene with human copies. (edubirdie.com)
In this study, we demonstrate that three independently derived founder mouse strains, transgenic for the TcR V gamma 1.1J gamma 4C gamma 4 (TcR gamma 4) chain gene, differed significantly from normal mice in their development of T and B cells as well as myelopoietic precursor cells. (pasteur.fr)
Ontogenic programs consistent with an acceleration of T-cell development and a delayed appearance and suppressed levels of pre-B- and B-cell precursors were evident in these transgenic mice. (pasteur.fr)
In addition, TcR gamma 4 transgenic mice possessed a significantly elevated level of myelopoietic pluripotential precursors. (pasteur.fr)
3H-thymidine cell suicide studies suggest that higher percentages of pluripotent precursors from the bone marrow of the TcR gamma 4 transgenic mice were in the S phase of the cell cycle. (pasteur.fr)
These modulations of the lymphoid and myelopoietic compartments, however, were not found in other T-cell receptor transgenic mice (e.g. (pasteur.fr)
Specifically, the trainee looked at the ability of an endothelial cell AGO 1 gene knockout to rescue streptozotocin-induced damage in female mice. (ca.gov)

This transgenic escape is worrisome because it can change the genomes of wild plants, eliminating genes that could be used to improve crops and possibly turn them into aggressive weeds, says Ralph Haygood of the University of Wisconsin-Madison in the northern U.S. state of Wisconsin. (cropchoice.com)
The latest version of Monsanto's suicide seeds won't even germinate unless exposed to a special chemical, while AstraZeneca's technologies outline how to engineer crops to become stunted or otherwise impaired if not regularly exposed to the company's chemicals. (jesus-is-savior.com)
Any attempt to release more transgenic crops in India would endanger not only farmers but the country's trade, exports and the environment as well. (sandarbha.com)

In India, it's been estimated that a farmer commits suicide every 30 minutes, typically by ingesting pesticide. (cairnsnews.org)
In my view, we should be horrified to realize that the business of growing food has gotten so financially challenging that even in a country like France a farmer commits suicide every two days 12 -again due to being financially ruined, just like the farmers in India. (cairnsnews.org)
Dr Vandana Shiva, physicist, philosopher, activist and winner of last year's Sydney Peace Prize, links more than 200,000 Indian farmer suicides to Monsanto's introduction of GM cottonseed in the early 1990s. (smh.com.au)
Higher debt levels have been responsible for a rapid increase in farmer suicides. (sandarbha.com)

Analysis of the available state wide and national data show that suicides among Indian cotton farmers increase with decreasing yield and net revenues. (gmwatch.org)
Farmers in Australia are committing suicide at alarming rates due to drought, poor commodity prices and the avaricious banks foreclosing on the family farm. (cairnsnews.org)
Why Do So Many Indian Farmers Commit Suicide? (cairnsnews.org)
The introduction of genetically engineered seeds, and the coercion of Indian farmers to use them, has led to the largest wave of recorded suicides in human history. (cairnsnews.org)
A year earlier, following approval by the Gene Technology Regulator, the WA government approved commercialisation of GM, or ''Roundup Ready'', canola - although their own fact sheet at the time cited a United Nations report that 'since the advent of GM canola in Canada farmers can no longer grow organic canola in western Canada. (smh.com.au)
The region is notorious for the huge numbers of suicides by primarily cotton farmers in the past 15 years and farmers' lobbies have been blaming the use of GM technology or Bt cotton as it is known for the spate of suicides. (activistpost.com)
The innumerable farmers' suicides due to unsustainable input costs leading to bankruptcy is another "feather" in the cap of the green revolution lobbyists! (vijayvaani.com)

In 1996 the first transgenic mouse models (R6/1 and R6/2) were developed (Mangiarini et al. (edubirdie.com)
1996) by inserting a part of the htt gene from a juvenile HD patient into the mouse genome. (edubirdie.com)

Schmeiser had spent 40 years perfecting his own canola hybrids when Monsanto genes were detected through 80 per cent of his crop. (smh.com.au)
Transgenic animal-human hybrids are exploited in a society utterly transformed by biotech. (sjgames.com)

Corporations such as Monsanto have backed away from the deployment of terminators for the time being while there has been an explosion in the development of novel use restriction technologies based on startling new discoveries in plant molecular genetics that led to the identification of homeotic genes that govern the pathways leading to cell differentiation. (i-sis.org.uk)
From genes to glaucoma: identifying glaucoma pathways. (hersenbank.nl)
Therefore, many genetically programmed cell suicide pathways have evolved to promote long-term survival of species from yeast to humans. (hopkinsmedicine.org)

When you transpose an alien gene (in this case from a soil habiting bacterium, Bacillus thurengiensis, known popularly as Bt) into a plant cell, targeting a specific pest, in this case the dreaded American Bollworm (the most devastating cotton pest), it is expected that the protein configuration which acts as a "poison" when in the gut of the sucking insect (boll worm), stays stable. (vijayvaani.com)

The forceful and illegal insertion of a transgenic crop into the biosphere of a region is an irreversible transgression, i.e. you cannot 'recall' a transgene like it were a defective car part. (activistpost.com)

Gene-environment interaction research and transgenic mouse models of Alzheimer's disease. (ox.ac.uk)

Herpes simplex virus thymidine kinase (HSV-tk) is a well-established suicide gene that has been successfully used to control GvHD following donor lymphocyte infusions (DLI) after allogeneic stem cell transplantation. (haematologica.org)
To investigate the in vitro effects of suicide gene therapy system of herpes simplex virus thymidine kinase gene (HSV-TK) in combination with the treatment of nucleotide analog-ganciclovir (GCV) on human pancreatic cancer, and to provide a novel clinical therapeutic method for human pancreatic cancer. (wjgnet.com)

I am appending a copy of my speech "Terminator Corporations' Suicide Seeds" a lecture presented at Sustainable World International Conference, 14-15 July 2005, London. (i-sis.org.uk)
A study by Haygood and others concluded there is no way to stop this leakage of transgenes into the environment, even with proposed and controversial ''suicide seed'' (Terminator) technology, designed to make farm-saved seeds sterile. (cropchoice.com)
The latter case describes the long and abusive history of Monsanto vis-à-vis the Indian people, beginning in 2001 with the discovery of a Monsanto-patented Bt gene within fields planted with conventional, non-GMO seeds in the western state of Gujarat. (activistpost.com)
The patents reveal that companies are developing suicide seeds whose genetic traits can be turned on and off by an external chemical 'inducer' -- mixed with the company's patented agrochemicals. (jesus-is-savior.com)

Gene silencing or knockout enables understanding of the role of a specific gene and associated mechanisms in certain disease conditions. (heraldopenaccess.us)
To reveal the role of RAGE signaling in AGE-dependent disorders, RAGE knockout cell lines and transgenic animals have been intensively studied [7-10]. (heraldopenaccess.us)

PCI substantially improves gene transfer in vitro not only with non-viral gene vectors, but, surprisingly, also with adenoviruses and adeno-associated viruses. (nih.gov)
T-cell receptor (TCR) gene transfer is an attractive strategy for rapid in vitro generation of high numbers of antigen specific T cells. (haematologica.org)

We used a replication defective recombinant retrovirus vector GINaTK (bearing HSV-TK gene) to make packaging cell PA317 produce progeny virions. (wjgnet.com)
My main scientific reasoning was that the recombinant gene technology used is a technique where there is much that is not clearly understood because it is at the very periphery of biological science. (vijayvaani.com)

Most gene therapy vectors are taken into the cell by endocytosis and, hence, are located in the endocytic vesicles. (nih.gov)
5 - 8 A drawback of TCR gene therapy is the formation of mixed TCR dimers, consisting of transgenic and endogenous TCR α and β chains. (haematologica.org)
Co-transfer of a suicide gene would provide a desirable safety switch in clinical TCR gene therapy. (haematologica.org)
A compact marker/suicide gene that utilizes established clinical-grade reagents and pharmaceuticals would be of considerable practical utility to T-cell cancer gene therapy. (ashpublications.org)
We predict that RQR8 will make T-cell gene therapy both safer and cheaper. (ashpublications.org)
Our data demonstrate that HSV-TK/GCV suicide gene therapy system is effective for treating experimental human pancreatic cancer, which is largely resistant to the common therapies, so the suicide gene therapy system may be a potential treatment approach for pancreatic cancer. (wjgnet.com)

2014). Although this study aims to find therapeutic benefit through nonallele-specific silencing of both the mutant and wild type huntingtin gene which codes for the huntingtin protein, it has been noted that the effects of nonallelle-specific silencing of the mutant htt still remains unknown. (edubirdie.com)

RNA-sequencing revealed that Adnpor Pogz deficiency induced prominent upregulation of overlapping genes enriched in neuroinflammation, similar to the elevation of pro-inflammatory genes in humans with neurodevelopmental disorders. (benrein.com)
1] Animal experimentation is going on all over the world the genes from chicken, cows, rats and humans have been engineered into commercial fish species in order to increase the growth rates- GM salmon, for instance, have been engineered to reach six times their natural size. (jesus-is-savior.com)

Autoimmune side effects, however, remain a risk, emphasizing the relevance of a suicide mechanism allowing in vivo elimination of infused T cells. (haematologica.org)
Adverse side effects, however, may occur and lead to severe autoimmunity or graft-versus-host disease (GvHD), emphasizing the relevance of a suicide mechanism that allows efficient in vivo elimination of infused T cells. (haematologica.org)
A larger project in Dr. Calderón-Urrea's laboratory, funded by ARI-CATI, addresses the possibility to use nematode cell suicide genes in transgenic plants as a mechanism to control nematode infestation. (fresnostate.edu)

There is lack of consideration for a large segment of the population that has ethical or religious beliefs that classify genetically engineered plants carrying animal or human genes as being totally unacceptable as foods. (jesus-is-savior.com)

It was discovered through transcript analysis that senescent cells have increased expression of pro-survival genes consistent with their resistance to apoptosis [6]. (lifespan.io)

We investigated the use of human CD20 as suicide gene in T-lymphocytes. (haematologica.org)
Human CD20 has been proposed as non-immunogenic protein with a dual function as selection marker and suicide gene. (haematologica.org)
Although human CD20 has previously been described as functional selection marker and suicide gene after retroviral transfer to T lymphocytes, 9 , 10 the specificities of these T cells were unknown, and therefore a potential effect of CD20 on antigen-specific T-cell functions could not be investigated. (haematologica.org)
Mouse HD genes are 81% in similarity to that of the human HD gene when expressed at DNA level (Liou, 2010). (edubirdie.com)
The research project is planning to put human genes into cows so that their milk is more like human breast milk. (jesus-is-savior.com)
In a well-received speech at a biotech conference in London in 2002, Avery claimed anti-biotech activists 'sided with terrorists' and formed 'human shields for Arafat, leader in chief of the suicide bombers. (gmwatch.org)
Two students focused on establishing transgenic mouse models to explore the impact of androgen receptor, hepatocyte growth factor and human c-MET on the development of prostate cancer tumors. (ca.gov)
Analysis of age-related changes in gene expression in human microglia. (hersenbank.nl)

Homeotic genes produce proteins that recognize regulatory genes called MADS-boxes that control genes for formation of reproductive tissue, leaves, branches, etc. (i-sis.org.uk)
We have reported that many insults can trigger cells to activate a cellular death pathway (Nature, 361:739-742, 1993), that several viruses encode proteins to block attempted cell suicide (Proc. (hopkinsmedicine.org)

resistance to Roundup, enabling fields to be soaked in the herbicide, and a terminator gene that renders them sterile. (smh.com.au)

Both ADNP and POGZ are chromatin regulators, which could profoundly affect gene transcription and cellular function in the brain. (benrein.com)
To approach this question a CRISPR-Cas9 approach was used to delete the SGF29 gene and assess its impact on cellular growth. (ca.gov)
94: 690-694, 1997), that cellular anti-death genes can alter the pathogenesis of virus infections (Nature Med. (hopkinsmedicine.org)

To understand the functional role of these neurodevelopmental disorder risk factors, we used viral-based gene transfer to investigate how Adnp or Pogz deficiency in mouse prefrontal cortex affects behavioural, transcriptomic and synaptic function. (benrein.com)
Microarray profiling of hypothalamic gene expression changes in Huntington's disease mouse models. (lu.se)
Effects of excitotoxicity in the hypothalamus in transgenic mouse models of Huntington disease. (lu.se)

The use of CD20 as suicide gene was investigated in CMV specific T cells and in T cells genetically modified with an antigen specific T-cell receptor. (haematologica.org)
Such techniques frequently use cell suicide toxins to abort formation of gametes or flowers and those toxins provide tangible hazards to those consuming food or feed. (i-sis.org.uk)
RQR8 is a 136-amino-acid epitope-based marker/suicide gene that enables clinical selection, cell tracking, and deletion in case of toxicity. (ashpublications.org)
knowledge of early stages of development will allow Calderón-Urrea to detect stages at which parasitic nematodes are most vulnerable to the action of cell death genes. (fresnostate.edu)
Accordingly, the epistatic relationship between cell death and SA accumulation, analyzed in crosses between lesion-mimic mutants (spontaneous lesion formation) and the transgenic nahG line (depleted in SA) places the SA activity in a feedback loop downstream and upstream of cell death. (springer.com)
The results suggest that the expression of a specific T-cell receptor gamma chain gene, and/or an elevated level of particular subset of TcR gamma delta cells, may affect the proliferation and relative proportions of haemopoietic and lymphoid precursors. (pasteur.fr)
Our final student worked to introduce a "suicide gene" construct into engineered in iPSCs for the generation of the insulin-producing -cell to treat type 1 diabetes patients. (ca.gov)
One example of a gene-edited cell line isthe soluble receptor for advanced glycation end product (sRAGE) secreting stem cells, and much attention is paid to the remedial role of sRAGE molecules in advanced glycation end product (AGE)-associated disorders [3-6]. (heraldopenaccess.us)

Attenuated huntingtin gene CAG nucleotide repeat size in individuals with Lynch syndrome. (lu.se)

Quantitative RT-PCR experiments also indicated that the native CAD gene was preferentially expressed in differentiating xylem both in stems and roots. (ox.ac.uk)

Their mental health has already been impaired by years of agricultural chemical use making them more susceptible to suicide and other serious illnesses. (cairnsnews.org)

Marker genes enable detection and selection of T cells, whereas suicide genes enable selective destruction of T cells in case of toxicity. (ashpublications.org)
Marker genes enable measurement of transduction and allow selection of transduced cells, whereas suicide genes allow selective deletion of administered T cells in the face of toxicity. (ashpublications.org)
We have created a highly compact marker/suicide gene for T cells combining target epitopes from both CD34 and CD20 antigens (RQR8). (ashpublications.org)

Sequence analysis and functional characterization of the promoter of the Picea glauca Cinnamyl Alcohol Dehydrogenase gene in transgenic white spruce plants. (ox.ac.uk)

Whole genome and brain gene expression. (nih.gov)

Recent evidence has suggested that gene-environment interactions (GxE) may play a crucial role in its development and progression. (ox.ac.uk)

Some aspects of the potential of PCI for site-specific gene delivery in therapeutic situations will also be discussed. (nih.gov)

The huntingtin gene which regulates brain-derived neurotrophic factor (BDNF) that promotes the survival of neurons in the brain is affected (Zuccato et al, 2010). (edubirdie.com)

DNA (a substance whose molecules carry the basic information for the growth and function of cells) from GEOs is also getting into the environment and becoming integrated into the gene make-up of wild plant relatives. (cropchoice.com)

This article will review the background for the PCI technology and its role for gene delivery using both non-viral and viral vectors. (nih.gov)

We then transferred the HSV-TK gene to target cells SW1990 using these progeny virions, and treated these gene-modified tumor cells with GCV to study the sensitivity of the cells to GCV and their bystander effects by routine MTT-method. (wjgnet.com)

Witte also co-discovered the gene for Bruton's tyrosine kinase , a protein essential for normal B-lymphocyte development that, when mutated, causes the onset of X-linked agammaglobulinemia . (ucla.edu)

With this research, 4 Prx genes (referred to as because ThPrxII, ThPrxIIE, ThPrxIIF, and Th2CysPrx) ended up cloned through Tamarix hispida. (sirtuinsignaling.com)

Twenty years ago GM, aka GE or transgenics, was sold much as nuclear power is sold today, as an evil necessary to meet demand. (smh.com.au)
Death happens within 10-20 years of diagnosis often by aspirational pneumonia on account of dis-coordinated swallowing or suicide. (edubirdie.com)
Scientist Phil L'Huillier who is heading the project, say's the first two or three years will be spent putting transgenic embryos into cows and breeding from them to produce transgenic calves which will in turn breed to produce herds of up to 30 animals for milking. (jesus-is-savior.com)

In this paper we present several environmental factors implicated in the etiology of AD that have been tested in transgenic animal models of the disease. (ox.ac.uk)

Histochemical observations of different vegetative organs of the transgenic trees showed that this sequence was sufficient to drive GUS expression in lignifying tissues, and more specifically in differentiating xylem cells. (ox.ac.uk)
This analysis led to the discovery of a population of preadipocyte cells whose gene expression is remodeled in an age-specific fashion. (ca.gov)
Understanding the activity of this gene will allow for an improved understanding of how cells can survive DNA damage leading to genomic rearrangements that support the initial stages of tumorgenesis and subsequent cancer development. (ca.gov)

Interestingly, poisoning by pesticide has actually become the leading method of suicide around the world, according to the World Health Organization. (cairnsnews.org)

Michael Specter tries to make light of such statistics stating that the Indian suicide trend is similar to that in France. (cairnsnews.org)

Here, we report the isolation and analysis of 5' flanking genomic DNA regions upstream to the CAD gene, from two conifers, i.e. white spruce (Picea glauca (Moench) Voss) and loblolly pine (Pinus taeda L.). Sequence comparisons with available CAD gene promoters from angiosperms highlighted the conservation of cis-elements matching MYB, WRKY and bHLH binding sites. (ox.ac.uk)

Future work in the lab will focus on identifying genes that induce DNA damage in response to short telomeres, identifying how telomeres are processed and how telomere elongation is regulated. (hopkinsmedicine.org)

As gene liberation depends on light, enhancement of gene expression is achieved only at illuminated regions. (nih.gov)

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