• These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. (nih.gov)
  • After infecting a cell, HIV instructs it to make viral proteins. (sciencedaily.com)
  • Tiny bits of these proteins, known as peptides, are displayed on the surface of the infected cell--the cell's way of signaling the immune system that it is infected. (sciencedaily.com)
  • 2013) The molecular basis for Mucosal-Associated Invariant T cell recognition of MR1 proteins. (scirp.org)
  • We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells sense normal and pathologically altered extracellular matrix. (mcgill.ca)
  • In cells lacking expression of Ca 2+ -mobilizing G proteins, coexpression of human GPR40 and Gα q allowed medium- and long-chain fatty acids to elevate intracellular [Ca 2+ ]. (aspetjournals.org)
  • In normal cells, folates are taken in through the reduced folate carrier (RFC) or proton-coupled folate transporter (PCFT), which are membrane-spanning proteins that facilitate bidirectional transportation of reduced folate across the plasma membrane and endosome membranes [ 1 ]. (biomedcentral.com)
  • The receptor also works insidethe cell as a shuttle craft, moving proteolytic enzymes to thelysosomes, a part of the cellular digestive system that breaksdown proteins into simpler compounds. (cancernetwork.com)
  • Specific high affinity binding proteins for THYROID HORMONES in target cells. (bvsalud.org)
  • The locus encoding the T cell antigen receptor (TCR) α-chain and δ-chain (Tcra-Tcrd) undergoes recombination of its variable-diversity-joining (V(D)J) segments in CD4(-)CD8(-) double-negative thymocytes and CD4(+)CD8(+) double-positive thymocytes to generate diverse TCRδ repertoires and TCRα repertoires, respectively. (duke.edu)
  • A chimeric antigen receptor (CAR) construct was also shown to be integrated in up to 60 percent of T cells. (businesswire.com)
  • B-cell antigen receptor complex-associated protein beta-chain precursor. (lu.se)
  • They act as mediators between multiple growth factor receptors that possess tyrosine kinase activity, such as the insulin and insulin growth factor receptors, and a complex network of intracellular signalling molecules, resulting in activation of, for example, the PI3K and RAS/ERK pathways and subsequent transcription of target genes. (atlasgeneticsoncology.org)
  • The GDNF family ligands (GFLs) function through a glycosyl-phosphatidylinositol-(GPI) anchored coreceptor, GDNF family receptor alpha (GFRα), and rearranged during transfection (RET), a well-known receptor tyrosine kinase involved in kidney development, spermatogonial stem cell maintenance, and the development and maintenance of the sympathetic, parasympathetic, and enteric nervous systems [ 1 , 2 ]. (medsci.org)
  • The integration of GPCR and receptor tyrosine kinase (RTK) signaling, including IR upon ligand stimulation, is eloquently reviewed. (mdpi.com)
  • Cell lines infected by HTLV-I express levels of TCR beta mRNA similar to PHA stimulated lymphocytes, suggesting that this gene is not transcriptionally activated as a result of infection by HTLV-I. Cloned T cells of known antigen specificity that are infected by HTLV-I in vitro show impairment of immune function, including loss of antigen-specific responsiveness and the acquisition of alloreactivity. (rupress.org)
  • Levels of expression of the TCR beta gene at the mRNA level and surface expression of the T3 complex were also not significantly altered, suggesting that changes in immune function cannot be attributed to quantitative changes in the TCR molecule. (rupress.org)
  • mRNA expressions were determined by real-time PCR using cDNA synthesized from whole-cell RNA, cells expressing T4 receptor mRNAs assessed by in situ hybridization, and KS disaccharide sulfation measured by electrospray ionization tandem mass spectrometry (ESI-MS/MS). (unboundmedicine.com)
  • keratocytes and endothelial cells expressed T4 receptor beta (THRB) mRNA. (unboundmedicine.com)
  • Although these receptors are structurally similar, they determine specificity for four ligands-GDNF, Neurturin (NRTN), Artemin (ARTN) and Persephin (PSPN). (medsci.org)
  • 2007) Structural basis of T-cell specificity and activation by the bacterial superantigen TSST-1. (scirp.org)
  • The specificity of T-cells is determined by the T-cell receptor (TCR), a heterodimer of alpha and beta protein chains. (elifesciences.org)
  • These HDI-responsive T cells expressed unique oligoclonal gamma/delta rather than alpha/beta T-cell receptors, with characteristics suggestive of antigen-mediated selection and specificity. (cdc.gov)
  • In TGF-beta-treated MCF10A mammary epithelial cells overexpressing HER2 and by chromatin immunoprecipitation, we identified novel Smad targets including protein tyrosine phosphatase receptor type kappa (PTPRK). (nih.gov)
  • Lens epithelial cells are distributed under the anterior capsule of the lens and under the capsule of the equator of the lens. (researchsquare.com)
  • The lens epithelial cells proliferate, differentiate and migrate inward to form lens fibers. (researchsquare.com)
  • Excessive proliferation and migration of lens epithelial cells and transformation into mesenchymal cells (ie EMT) are common causes of cataracts. (researchsquare.com)
  • Human T-cell lines were generated and characterized from peripheral blood of HDI-exposed and HDI-unexposed subjects, using two different HDI antigens, HDI-conjugated albumin and HDI-exposed human airway epithelial cells (NCI-H292). (cdc.gov)
  • Investigations into the molecular basis of CHI have led to the discovery of mutations in the sulfonylurea receptor and an inwardly rectifying potassium channel. (medscape.com)
  • TP53 is a significant tumor suppressor gene, and its somatic mutations are one of the most frequent alterations in ~50% of all human cancers, including colorectal cancer ( 10 ). (spandidos-publications.com)
  • Mutations in any of several genes are associated with familial TAAD. (medlineplus.gov)
  • Mutations in the ACTA2 gene have been identified in 14 to 20 percent of people with this disorder, and TGFBR2 gene mutations have been found in 2.5 percent of affected individuals. (medlineplus.gov)
  • Mutations in several other genes account for smaller percentages of cases. (medlineplus.gov)
  • Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
  • Next, the researchers began the arduous process of screening thelarge gene for one or more mutations that might disable it. (cancernetwork.com)
  • Usinga method to detect mismatches in genetic material, they comparedstrands of DNA from tumor cells and surrounding normal tissue,and discovered mutations in the tumor samples. (cancernetwork.com)
  • Cross-Sectional Study for the Detection of Mutations in the Beta-Globin Gene Among Patients with Hemoglobinopathies in the Bengali Population. (cdc.gov)
  • 80%) and biological pathways (such as adipocytokine, Notch, Hedgehog and NOD-like receptor signaling) were not identified by previous gene array studies. (mdpi.com)
  • We find that U87 mg electrotaxis is abolished under pharmacological inhibition of PI3Kγ, mTOR, AKT and ErbB2 signaling, whereas DAOY cell electrotaxis was not attenuated by these or other pathways evaluated. (nature.com)
  • G protein-coupled receptors (GPCR) have recently been implicated in intracellular crosstalk pathways with IR. (mdpi.com)
  • The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. (nih.gov)
  • Cadherin superfamily genes: functions, genomic organization, and neurologic diversity. (nih.gov)
  • The lab also focuses on how the extracellular matrix regulates key growth factors of the TGF-beta superfamily, and on deciphering regulatory mechanisms mediated by microRNAs. (mcgill.ca)
  • The protein encoded by this gene is a member of the TNF-receptor superfamily. (origene.com)
  • The majority of cases examined were clonal with respect to a particular TCR beta gene rearrangement. (rupress.org)
  • Wucherpfennig, K.W. and Hafler, D.A. (1995) A review of T-cell receptors in multiple sclerosis: Clonal expansion and persistence of human T-cells specific for an immunodominant myelin basic protein peptide. (scirp.org)
  • We also show the consistency of clonal expansion dynamics between bulk alpha and beta repertoires, using a new methodology to reconstruct alpha-beta pairings from clonal trajectories. (elifesciences.org)
  • DNA sequence analysis of T-cell receptors was used to further define clonal populations of HDI-responsive T cells. (cdc.gov)
  • Glial cell line-derived neurotrophic factor family receptor alpha (GFRα) members have been widely connected to the mechanisms contributing to cell growth, differentiation, cell migration and tissue maturation. (medsci.org)
  • The glial cell line-derived neurotrophic factors (GDNFs), a family of neurotrophic factors, were initially thought to be able to regulate the growth, survival, and differentiation of neural-derived cell types. (medsci.org)
  • Based on whether it cooperates with the second receptor RET, GFRα has also been widely linked to the mechanisms that contribute to cell growth, differentiation and migration and tissue maturation. (medsci.org)
  • therefore, definitive differentiation of Hürthle cell carcinoma from Hürthle-cell adenoma is based on vascular invasion and/or capsular invasion, as well as on permanent histologic sections or extrathyroidal tumor spread and lymph node and systemic metastases. (medscape.com)
  • These receptors are activated by hormones that leads to transcription, cell differentiation, and growth suppression. (bvsalud.org)
  • Expression of IRS1 , IRS2 or IRS4 in the 32D haematopoietic cell line leads to proliferation of the myeloid progenitor cells and expression of activated IRS4 has recently been demonstrated in the human hepatoblastoma cell line HepG2, with inhibition of IRS4 resulting in diminished growth. (atlasgeneticsoncology.org)
  • We report that haploinsufficiency for the forkhead transcription factor Foxo1 reverses beta cell failure in Irs2(-/-) mice through partial restoration of beta cell proliferation and increased expression of the pancreatic transcription factor pancreas/duodenum homeobox gene-1 (Pdx1). (nih.gov)
  • We propose that insulin/IGFs regulate beta cell proliferation by relieving Foxo1 inhibition of Pdx1 expression in a subset of cells embedded within pancreatic ducts. (nih.gov)
  • Transforming growth factor beta (TGF-beta) inhibits proliferation and promotes cell migration. (nih.gov)
  • Endogenous RPTPkappa associated with EGF receptor and HER2, resulting in suppression of basal and ErbB ligand-induced proliferation and receptor phosphorylation. (nih.gov)
  • Combined with simple proliferative responses, the identified nucleotides are also shown to have functional consequences by inducing T cell proliferation for CD4/V β 20-1 + T cells, while failing to induce proliferation in other T cell isolates. (scirp.org)
  • The CCK-8 method measures cell proliferation, and the Transwell method detects migration and invasion. (researchsquare.com)
  • HDI antigen preparations but not "mock exposed" control antigens lead to increased proliferation of specific cell types, CD3+CD4-CD8(dim) and/ or CD3+CD4-CD8- cells, from HDI-exposed but not from HDI-unexposed subjects. (cdc.gov)
  • DNA polymorphisms at the BCL11A, HBS1L-MYB, and beta-globin loci associate with fetal hemoglobin levels and pain crises in sickle cell disease. (cdc.gov)
  • The relationship between VDR gene polymorphisms and onset pattern of diabetes was not significant. (who.int)
  • We present a rate equation model for the TGF-b pathway in endothelial cells together with novel measurements. (lu.se)
  • endothelial cells. (lu.se)
  • Drugs and drug-like molecules can modulate the function of mucosal-associated invariant T cells. (expasy.org)
  • 2012) Structural insight into MR1-mediated recognition of the mucosal associated invariant T cell receptor. (scirp.org)
  • Configuration and expression of the T cell receptor beta chain gene in human T-lymphotrophic virus I-infected cells. (rupress.org)
  • We studied the configuration and expression of the gene encoding the beta chain of the T cell receptor (TCR beta) in cell lines and primary tumor cells infected by the human T cell leukemia/lymphoma (lymphotrophic) virus type I (HTLV-I). Most of the cell lines and all the primary tumor cells showed rearrangement of the TCR beta gene, and in each case the rearrangement was distinct. (rupress.org)
  • One feature of inflammation-associated gut microbiotas is enrichment of motile bacteria, which can facilitate microbiota encroachment into the mucosa and activate pro-inflammatory gene expression. (nature.com)
  • The link between elevated microbiota flagellin levels and intestinal inflammation is thought to involve flagellin's ability to activate pro-inflammatory gene expression via TLR5 and the NLRC4 inflammasome. (nature.com)
  • The role of exosomes collected by ultracentrifugation were also analyzed as mediators of p53 expression in cancer cells and APJ expression in fibroblasts. (spandidos-publications.com)
  • APJ expression in fibroblasts co‑cultured with p53‑suppressed colon cancer cells (HCT116 sh p53 cells) was significantly lower than in control colon cancer cells (HCT116 sh control cells). (spandidos-publications.com)
  • MicroRNA 5703 enriched in exosomes derived from HCT116 sh p53 cells inhibited APJ expression, and inhibition of miR‑5703 diminished APJ suppression in fibroblasts caused by cancer cells. (spandidos-publications.com)
  • Regulation of bacterial virulence gene expression by the host environment. (jci.org)
  • We are interested in the molecular basis for developmentally regulated rearrangement and expression of murine TCR genes. (duke.edu)
  • Foxo1 and Pdx1 exhibit mutually exclusive patterns of nuclear localization in beta cells, and constitutive nuclear expression of a mutant Foxo1 is associated with lack of Pdx1 expression. (nih.gov)
  • Functionality of HSV-TK gene expressing T-cells was shown by loss of bcr-able gene expression as well as by control of cytomegalovirus-reactivation. (frontiersin.org)
  • After expression of GPR40-Gα q in HEK293 cells and membrane preparation basal binding of [ 35 S]GTPγSinGα q immunoprecipitates was high and not elevated substantially by fatty acids. (aspetjournals.org)
  • Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in TRα or TRβ activity. (thomassci.com)
  • The expression levels of Gα subunits influence the biased profiling of β-agonists and antagonists in that they determine both their activity and efficacy by affecting different membrane distribution of receptor-G protein populations. (mdpi.com)
  • In the naïve state, the level of Gα expression influences the partitioning of not only Gα but also the co-expressed receptor in different membrane domains. (mdpi.com)
  • MicroRNAs (miRNAs) are small non-coding RNAs responsible for posttranscriptional regulation of gene expression. (lu.se)
  • PCR was performed to detect the expression of the protease-activated receptors (PARs) in MSCs. (biomedcentral.com)
  • AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. (origene.com)
  • The data presented is normalized to the expression of Rpl32 , a housekeeping gene. (cdc.gov)
  • CHRNA5 expression research findings from the laboratory to CHRNA5 was among the markers with in bronchial cel s modulates cell the population. (who.int)
  • CHRNA3 gene expression disease associations. (who.int)
  • Although Hürthle cell carcinoma was previously considered a variant of follicular cell neoplasms, which are generally less aggressive, the 2017 World Health Organization (WHO) classification of endocrine tumors reclassified it as a distinct entity. (medscape.com)
  • G protein stoichiometry can dictate biased agonism through distinct receptor-G protein partitioning. (mdpi.com)
  • Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. (origene.com)
  • Transitional dendritic cells are distinct from conventional DC2 precursors and mediate proinflammatory antiviral responses. (stanford.edu)
  • When working properly, the M6P/IGF2r receptor protein has at leastthree distinct regulatory roles within a cell, said Jirtle. (cancernetwork.com)
  • whether a distinct receptor for C4a is present is unclear (see Table 1). (medscape.com)
  • Some investigators believe that this condition is distinct from other follicular cell neoplasms. (medscape.com)
  • Configuration of immunoglobulin and T cell receptor beta and gamma genes in acute myeloid leukaemia: pitfalls in the analysis of 40 cases. (bmj.com)
  • The Human and Mouse T-cell Receptors Panel is used for sequencing the V(D)J region of the alpha, beta, delta and gamma genes, including the CDR3 regions. (qiagen.com)
  • ABSTRACT Reported associations between vitamin D receptor (VDR) polymorphism and type 1 diabetes mellitus vary across ethnic groups. (who.int)
  • INDIGO's panel of TR reporter assays utilizes non-human mammalian cells engineered to express human thyroid hormone receptors alpha (NR1A1) and beta (NR1A2), ligand-dependent transcription factors commonly referred to as TRα and TRβ. (thomassci.com)
  • The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. (origene.com)
  • This gene has also been linked to a susceptibility to type I diabetes in women. (origene.com)
  • Development of asthma is multifactorial and depends on the interactions among multiple susceptibility genes and environmental factors. (msdmanuals.com)
  • Engler, O.B., Strasser, I., Naisbitt, D.J., Cerny, A. and Pichler, W.J. (2004) A chemically inert drug can stimulate T cells in vitro by their T cell receptor in non-sensitised individuals. (scirp.org)
  • Methodologically, the lab employs a wide spectrum of techniques ranging from in vitro biochemical and biophysical assays, to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo studies with genetically modified mouse models. (mcgill.ca)
  • However, the quantity of MSCs is very low in bone marrow (about 0.001 to 0.01% of the mononuclear cells) and in vitro expansion is the prerequisite for their clinical application. (biomedcentral.com)
  • Antibody-dependent cell-mediated cytotoxicity (ADCC) was evaluated with FRβ positive CHO cells as target cells and isolated peripheral blood monocytes as effector cells in an in vitro assay. (biomedcentral.com)
  • Although other irritants in the smoke may have contributed to the incident, there is supporting evidence that stable strontium can stimulate the release of histamine from mast cells in vitro (ATSDR 2001e). (cdc.gov)
  • The investigators showed that a class of antimalarial drugs known as artemisinins, typified by the FDA-approved drug artemether , were capable of inhibiting ARX levels, reducing glucagon, and increasing insulin in an in vitro alpha-cell model. (medscape.com)
  • Folate receptor beta (FRβ) is only detectable in placenta and limited to some hematopoietic cells of myeloid lineage in healthy people. (biomedcentral.com)
  • Merging computational and simple cell assays, this work establishes a role of nucleotides in T cells found to contain this TCR subtype. (scirp.org)
  • INDIGO Bioscience's nuclear receptor reporter assays are all-inclusive cell-based assay systems. (thomassci.com)
  • The t(X;7)(q22;q34) in paediatric T-cell acute lymphoblastic leukaemia results in overexpression of the insulin receptor substrate 4 gene through illegitimate recombination with the T-cell receptor beta locus. (atlasgeneticsoncology.org)
  • For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells. (bdbiosciences.com)
  • After someone is infected with HIV, a subgroup of their immune cells known as CD8 cytotoxic T lymphocytes, or CTLs, recognize cells infected with HIV and kill them before they become HIV-producing factories. (sciencedaily.com)
  • Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P ) and either (A) Mouse Anti-Human CD8a Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB1509R) or (B) Mouse IgG 2B Alexa Fluor 647 Isotype Control (Catalog # IC0041R ). (rndsystems.com)
  • The identification of lymphocytes as the main cell type responsible for both cellular and humoral immunity started in the early 1950s with the emergence of cell culture techniques. (frontiersin.org)
  • The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. (wikidoc.org)
  • The present study was conducted to determine whether corneal cells differentially express genes for T4 regulation, keratan sulfate proteoglycan (KSPG) synthesis, crystallins, and endothelial cell ion transporters during transparency development and whether these expressions are altered when E9 embryos are treated with T4. (unboundmedicine.com)
  • This has suggested a potential role for GPR40 in the regulation of β-cell responsiveness to free fatty acids and hence as a potential therapeutic target in the regulation of insulin secretion and diabetes. (aspetjournals.org)
  • In my goal to understand beta- and alpha-cell regulation, we have lately demonstrated the novel finding that the chloride channel CFTR is present in both mouse and human beta- and alpha-cells. (lu.se)
  • In alpha-cells, CFTR is important for regulation of alpha-cell electrical activity and the generation of action potentials. (lu.se)
  • Rearrangement of the genes for the beta and gamma chains of the T cell receptor is rarely observed in adult B cell lymphoma and chronic lymphocytic leukemia. (jci.org)
  • In 7 of the 100 B cell specimens, a single nongermline band was detected after digestion with the restriction enzyme BamHI, but the rearrangement could be confirmed with a second restriction enzyme in only two. (jci.org)
  • A rearrangement of the T gamma gene was never seen in a B cell sample. (jci.org)
  • In contrast, T cell specimens usually rearranged both alleles of T beta (15 of 18), the rearrangement could be confirmed with a second restriction enzyme (17 of 18), both alleles of the first constant region gene segment of T beta always underwent either rearrangement or deletion, and the T gamma gene was also rearranged or deleted (17 of 18). (jci.org)
  • We conclude that ordered rearrangement of the T cell receptor is a rare event in B cell lymphoma and B-CLL. (jci.org)
  • This suggests that the chromosomal abnormality, and possibly infection by HTLV-I, occurred before TCR beta gene rearrangement. (rupress.org)
  • In beta-cells, CFTR act as a regulator of the Ca 2+ activated chloride channel ANO1 to regulate granular priming and exocytosis of insulin granules. (lu.se)
  • To develop dynamic magnetic field generation in combination with usage of superparamagnetic nanoparticles as a means to regulate islet cell functions. (lu.se)
  • This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. (nih.gov)
  • Linkage and comparative mapping studies indicate that the transferred chromosome segment contains a number of candidate genes for hypertension, including genes encoding a brain dopamine receptor and a renal epithelial potassium channel. (jci.org)
  • These findings demonstrate that BP regulatory gene(s) exist within the differential chromosome segment trapped in the SHR-Lx congenic strain and that this region of chromosome 8 plays a major role in the hypertension of SHR vs. BN-Lx rats. (jci.org)
  • The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. (origene.com)
  • Others and we suggest that the beta cell release primed insulin granules during first phase insulin secretion, which is lacking in individuals with pre-diabetes. (lu.se)
  • The protein receptor produced by M6P/IGF2r is an attractive targetbecause it is present on the cell surface and in the plasma, Jirtlesaid, making it readily accessible for use in both liver tumortherapy and diagnosis. (cancernetwork.com)
  • The human and murine protocadherin-beta one-exon gene families show high evolutionary conservation, despite the difference in gene number. (nih.gov)
  • Various genetic abnormalities such as wingless and int-1 (Wnt) signaling, tissue growth factor beta (TGFβ) signaling, or TP53 signaling have been known in the carcinogenesis of colorectal cancer ( 2 , 3 ). (spandidos-publications.com)
  • EMERYVILLE, Calif.--( BUSINESS WIRE )--Metagenomi, a genetic medicines company with a versatile portfolio of next-generation gene editing tools, today announced that the company will share data related to their novel, compact, and hypo-immune gene editing systems at the 63rd Annual Meeting and Exposition of the American Society of Hematology (ASH), which is taking place in Atlanta, GA and virtually, December 11-14. (businesswire.com)
  • Two genetic variants in strong linkage disequilibrium (rs9536314 and rs9527025) in the Klotho (KL) gene, encoding a transmembrane protein, implicated in longevity and associated with brain resilience during normal aging, were recently shown to be associated with Alzheimer disease (AD) risk in cognitively normal participants who are APOE ε4 carriers. (lu.se)
  • Sickle cell anemia - Nitric oxide related genetic modifiers of hematological and biochemical parameters. (cdc.gov)
  • We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
  • To further understand electrotactic migration of tumor cells, we performed RNA-sequencing for pathway discovery to identify signaling that is differentially affected by the exposure of direct-current electrical fields. (nature.com)
  • In MCF10A/HER2 cells, TGF-beta enhanced cell motility, FAK phosphorylation, F-actin assembly, and focal adhesion formation and inhibited RhoA activity. (nih.gov)
  • Of the 8 plasma membrane receptors for complement, only deficiencies of CR3 and CR4 due to CD18 deficiency have been described, known as leukocyte adhesion deficiency (LAD) type 1 . (medscape.com)
  • The CD11/CD18 complex is part of the beta-2 integrin family and is important in adhesion and phagocytosis (see Table 1). (medscape.com)
  • Novel CRISPR-Associated Gene Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genomic Engineering for Cell Therapy Development," three novel gene editing systems were used to make reproducible and efficient edits to human immune cells, demonstrating utility for the next generation of cell therapy development for blood cancers. (businesswire.com)
  • Novel gene editing systems were deployed in NK cells to disrupt CD38 - a cell surface immune modulator that can be targeted in the development of cancer immunotherapy - and to integrate a CAR construct that led to robust CAR-directed cellular cytotoxicity. (businesswire.com)
  • In summary, these novel systems were shown to result in highly efficient and specific gene edits in human immune cells and display the potential for use in cell therapy development. (businesswire.com)
  • We adapt and forge these naturally evolved systems into powerful gene editing systems that are ultra-small, extremely efficient, highly specific and have a decreased risk of immune response. (businesswire.com)
  • By outfitting immune-system killer cells with a new pair of genes, scientists have transformed them into potent weapons that destroy cells infected with HIV, the virus that causes AIDS. (sciencedaily.com)
  • Their novel strategy of genetically engineering immune cells to redirect their infection-fighting ability toward killing HIV-infected cells could lead to an entirely new approach for combating AIDS and other viral diseases. (sciencedaily.com)
  • But then -- largely because these CTLs may not bind tightly enough to the infected cells or because HIV mutates so rapidly -- the virus typically evades and ultimately overpowers the immune system, leading to an increase in viral load that, in the absence of drug therapy, results in AIDS. (sciencedaily.com)
  • The QIAseq Immune Repertoire RNA Library Kits use Unique Molecular Indices (UMI) with gene-specific primers to target specific RNAs for NGS sequencing. (qiagen.com)
  • The data analysis included with the purchase of the QIAseq Immune Repertoire T-cell receptor panels includes an online portal that seamlessly integrates with Illumina BaseSpace and provides primary read mapping, UMI demultiplexing and reports on sequencing performance, TCR chain usage, CDR3 peptide sequence and length distributions, together with rarefaction and V/D/J usage heat maps. (qiagen.com)
  • The QIAseq Immune Repertoire RNA Library Kit relies on a highly efficient, TCR-specific cDNA synthesis reaction, ligation of sample index adapters containing UMIs and TCR gene-specific primer enrichment for sensitive TCR clonotype and diversity assessment. (qiagen.com)
  • Allergic reactions to drugs and chemicals are mediated by an adaptive immune response involving specific T cells. (frontiersin.org)
  • However, attention has recently turned to the naive T-cell repertoire, since it may largely determine the efficacy of the induced immune response ( 22 ). (frontiersin.org)
  • It is now clear that the ability of T cells to promote an effective immune response depends on a large repertoire of unique T-cell receptors (TCRs) generated and selected in the thymus. (frontiersin.org)
  • The diverse repertoire of T-cell receptors (TCR) plays a key role in the adaptive immune response to infections. (elifesciences.org)
  • T-cells play a crucial role in the immune response to pathogens by mediating antibody formation and clearance of infected cells, and by defining an overall response strategy. (elifesciences.org)
  • The resulting diverse naïve repertoire contains T-cell clones that recognize epitopes of yet unseen pathogens, and can participate in the immune response to infection or vaccination. (elifesciences.org)
  • Separation of normal and immune lymphoid cells by antigen-coated coated columns. (wikidata.org)
  • Alloantiserum-induced inhibition of immune response gene product function. (wikidata.org)
  • The human immune response to isocyanate , a leading cause of occupational asthma, remains incompletely characterized, including the cell types involved and the form of the chemical that acts as an antigen. (cdc.gov)
  • These data are the first to demonstrate that HDI can selectively stimulate gamma/delta T cells with the potential to modulate the human immune response to exposure. (cdc.gov)
  • This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. (origene.com)
  • immune destruction of insulin-pro- vestigate the relationship of VDR gene ducing beta cells by T-cell infiltration polymorphism to the risk of type 1 DM DNA isolation and PCR experiments of the pancreatic islets [1]. (who.int)
  • Using TCR alpha and beta repertoire sequencing for T-cell subsets, as well as single-cell RNAseq and TCRseq, we track the concentrations and phenotypes of individual T-cell clones in response to primary and secondary yellow fever immunization - the model for acute infection in humans - showing their large diversity. (elifesciences.org)
  • Komatsu, T., Moriya, N. and Shiohara, T. (1996) T cell receptor (TCR) repertoire and function of human epidermal T cells: Restricted TCR V alpha-V beta genes are utilized by T cells residing in the lesional epidermis in fixed drug eruption. (scirp.org)
  • 1994) T cell receptor V alpha repertoire of infiltrating T cells in labial salivary glands from patients with Sjogren's syndrome. (scirp.org)
  • 1992) T cell receptor repertoire of infiltrating T cells in lips of Sjogren's syndrome patients. (scirp.org)
  • Drug and Chemical Allergy: A Role for a Specific Naive T-Cell Repertoire? (frontiersin.org)
  • On the other hand, a large T-cell repertoire found in multiple donors would underline the potential of a drug/chemical to be recognized by many donors. (frontiersin.org)
  • These observations may also suggest that in the case of drug/chemical allergy, the T-cell repertoire results from particular properties of certain TCR to recognize hapten-modified peptides without need for previous thymic selection. (frontiersin.org)
  • The aim of this review is to address the existence and the role of a naive T-cell repertoire in drug and chemical allergy. (frontiersin.org)
  • The overall outcome of the thymic selection is the maintenance of a T-cell repertoire that has sufficient, but not too strong, affinity for any self-pMHC complex ( 29 ). (frontiersin.org)
  • The MR9-4 monoclonal antibody specifically recognizes the Vβ 5.1 and Vβ 5.2 T-cell Receptors of strains having the b haplotype (e.g. (bdbiosciences.com)
  • APJ suppression from a specific microRNA in cancer cell‑derived exosomes induced CAF‑like properties in fibroblasts. (spandidos-publications.com)
  • On the other hand, T cells constitute the main pathogenic effector cells in delayed hypersensitivity reactions ( 4 - 6 ). (frontiersin.org)
  • Formation of palindromic (P) region at the variable (V)-diversity (D)-joining (J) junction in DNA polymerase beta (pol-beta) deficient mice were investigated by sequencing of reverse transcriptase-polymerase chain reaction (RT-PCR) products of mRNAs encoding the beta chain of T cell receptor (TCR). (neb.com)
  • To date, six patients have relapsed and died, two after a second hematopoietic stem cell transplantation without T-cell depletion or administration of unmodified T-cells. (frontiersin.org)
  • Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is applied successfully to the treatment of many hematopoietic malignancies, but remains limited by severe acute graft-versus-host-disease (aGvHD). (frontiersin.org)
  • TGF-Beta codon 25 polymorphism and the risk of graft-versus-host disease after allogenic hematopoietic stem cell transplantation. (cdc.gov)
  • The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. (nih.gov)
  • T cell receptor beta constant 1 is a protein that in humans is encoded by the TRBC1 gene. (wikipedia.org)
  • High-dimensional approaches have revealed heterogeneity amongst dendritic cells (DCs), including a population of transitional DCs (tDCs) in mice and humans. (stanford.edu)
  • For example, how can genes be delivered at the precise dose to cells in humans? (medscape.com)
  • We determined the configuration of the genes for the beta (T beta) and gamma (T gamma) chains of the T cell receptor in DNA from 100 consecutive cases of B cell lymphoma and B cell chronic lymphocytic leukemia (B-CLL), and compared the findings with those in 18 T cell neoplasms. (jci.org)
  • Acute lymphoblastic leukemia (ALL), Multiple myeloma (MM), and Chronic lymphocytic leukemia (CLL), and Diffuse Large B-Cell Lymphoma (DLBCL). (codemap.com)
  • RESULTS: Four cases of post-cardiac-transplant folliculotropic mycosis fungoides (fMF) and one case of peripheral T-cell lymphoma (PTCL) were positive in off-target reads for MCPyV DNA. (bvsalud.org)
  • TP53‑deficiency in cancer cells is associated with robust stromal activation. (spandidos-publications.com)
  • Diabetes is caused by an absolute (type 1) or relative (type 2) deficiency of insulin-producing beta cells. (nih.gov)
  • The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. (wikidoc.org)
  • Watkins, S.L. and Pichler, W.J. (2013) Activating interactions of sulfanilamides with T cell receptors. (scirp.org)
  • The dynamics of cell-extracellular matrix interactions in normal and pathological situations. (mcgill.ca)
  • The Reinhardt laboratory is interested in components and mechanisms involved in the biogenesis, function and pathological aspects of the extracellular matrix and its interactions with cells. (mcgill.ca)
  • This gene encodes the CD8 alpha chain isoforms. (wikidoc.org)
  • Hürthle cell carcinoma of the thyroid gland is an unusual and relatively rare type of differentiated thyroid cancer. (medscape.com)
  • Hürthle cells are observed in both neoplastic and nonneoplastic conditions of the thyroid gland (eg, Hashimoto thyroiditis , nodular and toxic goiter ). (medscape.com)
  • [ 2 ] Oncocytic cells in the thyroid are often called Hürthle cells, and oncocytic change is defined as cellular enlargement characterized by an abundant eosinophilic granular cytoplasm as a result of accumulation of altered mitochondria. (medscape.com)
  • Thyroid hormone receptors are encoded by two genes (GENES, ERBA): erbA-alpha and erbA-beta for alpha and beta thyroid hormone receptors, respectively. (bvsalud.org)
  • A Hürthle cell neoplasm is defined generally as an encapsulated thyroid lesion comprising at least 75% Hürthle cells. (medscape.com)
  • The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. (wikidoc.org)
  • My research focus is to understand how we can circumvent the reduced capacity of the pancreatic beta cells to secrete enough insulin, and why the control of alpha cell glucagon secretion becomes disturbed in diabetes. (lu.se)
  • T cell receptor gene studies allow B and T cell lymphomas to be distinguished from each other and from common acute lymphoblastic leukemia antigen-positive non-T, non-B acute lymphoblastic leukemia. (jci.org)
  • A diagnose of T-cell acute lymphoblastic leukaemia was made. (atlasgeneticsoncology.org)
  • Thus, prophylactic transfusion of donor T-cells has been included in many protocols, despite the increased risk for acute GvHD ( Kolb, 2008 ). (frontiersin.org)
  • Total 42 and 43 cDNA clones encoding V(beta8)-D(beta)-J(beta)-C(beta) from E18.5 embryonic thymocytes of pol-beta gene knocked-out and wild type control mouse, respectively, were sequenced. (neb.com)
  • Among them five and six clones from pol-beta knocked-out and wild type, respectively, have P insertions of two nucleotides. (neb.com)
  • This result unequivocally indicates that pol-beta, which is one of the repair-type DNA polymerases most abundantly expressed in thymus and spleen, is not essential for the formation of P region. (neb.com)
  • Then, as a way to efficiently insert both genes into "naïve" CTLs (from people not infected with HIV), they developed an efficient delivery system in which the genes were combined and packaged inside a special type of virus, called a lentivirus. (sciencedaily.com)
  • 2004) T cell receptors recognizing type II collagen in HLA-DR-transgenic mice characterized by highly restricted V beta usage. (scirp.org)
  • On one hand, drug-specific T cells provide the necessary help for mounting an effective B-cell response observed in immediate-type hypersensitivity reactions. (frontiersin.org)
  • In type 1 diabetes , beta cells succumb to autoimmunity, leaving individuals with significantly reduced beta-cell mass and a lifelong dependence on insulin replacement therapy. (medscape.com)
  • In type 2 diabetes , beta cells fail to secrete sufficient insulin to overcome the prevailing insulin resistance . (medscape.com)
  • Although a few FDA-approved therapies for type 2 diabetes (meglitinides, sulfonylureas, incretin-based drugs) directly enhance beta-cell function, no therapies for any form of diabetes lead to the growth of healthy, new beta cells to replace those that were lost or dysfunctional. (medscape.com)
  • [ 3 ] Clinical trials are currently under way on the safety, tolerability, and efficacy of transplanting such cells in encapsulated forms in persons with type 1 diabetes, with results to be announced in the coming years. (medscape.com)
  • RÉSUMÉ Les associations observées entre le polymorphisme du récepteur de la vitamine D et le diabète de type 1 varie en fonction des groupes ethniques. (who.int)
  • Un groupe de 69 patients atteints de diabète de type 1 et 45 témoins en bonne santé sans lien entre eux ont été recrutés pour cette étude. (who.int)
  • Detecting virus-infected cells so they can then be eliminated is the job of CTLs and the protein molecules, known as T-cell receptors, that jut from their surface. (sciencedaily.com)
  • This discrepancy is explained by thymic selection where the fate of T-cell precursors is dependent on the recognition of self-peptides (self-p) presented by MHC molecules on thymic stromal cells ( 28 ). (frontiersin.org)
  • Are there ways to mimic the effects of genes with hormones or small molecules? (medscape.com)
  • Localization of Merkel cell polyomavirus (MCPyV) RNA was confirmed by RNAScope in situ hybridization. (bvsalud.org)
  • The configuration of the TCR beta gene in primary tumor cells infected by HTLV-I was compared with that in the derived cell lines. (rupress.org)
  • In all pairs examined, the configuration in the primary tumor cells was different from that in the cell lines, strongly suggesting that the cells that grow in culture are not the original neoplastic cells. (rupress.org)
  • The mutational inactivation of the TP53 gene in tumor cells has been reported to affect not only tumor cells but also the surrounding cells in the tumor microenvironment and to promote tumor-stromal activation and subsequent tumor growth ( 11 , 12 ). (spandidos-publications.com)
  • Given the lack of access, and the dispersed nature of brain tumor cells, we explore the possibility of electric fields inducing directed tumor cell migration. (nature.com)
  • The mechanisms governing replication of terminally differentiated beta cells and neogenesis from progenitor cells are unclear. (nih.gov)
  • Both alpha and beta chains share significant homology to immunoglobulin variable light chains. (wikidoc.org)