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  • Circuits
  • A synthetic biology approach that adds immunostimulatory gene circuits to cancer cells may contribute to combination immunotherapies against cancer. (genengnews.com)
  • Approaching the immune system as though it were a work of engineering in need of improved cancer-fighting programming, MIT scientists have developed gene circuits that alter gene expression in cancer cells, causing the cancer cells to come to the notice of T cells, which respond by killing the cancer cells selectively, sparing healthy cells. (genengnews.com)
  • The installation of synthetic gene circuits that work only in cancer cells represents a new form of cancer immunotherapy, which has shown considerable potential, but still faces stubborn challenges. (genengnews.com)
  • Lu and colleagues described their work October 19 in Cell , in an article entitled "Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer Immunotherapy. (genengnews.com)
  • System
  • There has been a lot of clinical data recently suggesting that if you can stimulate the immune system in the right way you can get it to recognize cancer,' said Lu, who is head of the Synthetic Biology Group in MIT's Research Laboratory of Electronics. (genengnews.com)
  • scientists
  • The scientists say that their synthetic biology approach, which has been shown to work in an in vivo model of ovarian cancer, as well as in an animal model, can be tweaked to recognize other cancers. (genengnews.com)
  • circuit
  • To do this, Lu and a team including MIT postdocs Lior Nissim, Ph.D., and Ming-Ru Wu, M.D., Ph.D., have built a gene circuit encoded in DNA designed to distinguish cancer cells from noncancer cells. (genengnews.com)
  • mammalian
  • Potentially, this strategy can be applicable to other mammalian genes. (umbc.edu)
  • To reset the endogenous thyrotrophic feedback control, we designed a synthetic mammalian gene circuit that maintains thyroid hormone homeostasis by monitoring thyroid hormone levels and coordinating the expression of a thyroid-stimulating hormone receptor antagonist (TSH Antag ), which competitively inhibits the binding of thyroid-stimulating hormone or the human autoantibody to TSHR. (pnas.org)
  • biology approach
  • The scientists say that their synthetic biology approach, which has been shown to work in an in vivo model of ovarian cancer, as well as in an animal model, can be tweaked to recognize other cancers. (genengnews.com)
  • Based on comparative genome analysis and data mining, we herein present a blueprint to custom design and engineer the entire baculovirus genome for optimized production properties using a bottom-up synthetic biology approach. (ovid.com)
  • synthesis
  • This support from the TSB follows the successful development by Isogenica of its Colibra™ technology for the commercial synthesis of ratio controlled gene libraries using a patent protected methodology discovered by Dr Anna Hine of Aston University which was also supported by TSB funding. (businesswire.com)
  • genome
  • Gene expression actualizes the organismal phenotypes encoded within the genome in an environment-dependent manner. (nih.gov)
  • Southern analysis determined that the magainin genes had integrated into the potato genome. (lincoln.ac.nz)
  • Recently, synthetic biology approaches have begun to make their mark in improving existing BEVS reagents by de novo design of streamlined transfer plasmids and by engineering the baculovirus genome. (ovid.com)
  • fluorescence
  • Following fitness and fluorescence measurements in two distinct environments (inducer alone and antibiotic alone), we applied a computational approach to predict cell population fitness and subpopulation fractions in the combination of these environments based on stochastic cellular movement in gene expression space and fitness space. (nih.gov)
  • recombinant
  • In particular, using P. pastoris cells, the synthetic gene was functionally overexpressed, allowing for the first time to produce recombinant Lipl of high purity at a level of 150 U/mL culture medium. (umbc.edu)
  • bespoke
  • The discovery may widen the toolkit of synthetic biologists trying to build bespoke organisms. (newscientist.com)
  • Isogenica's Colibra™ technology allows the construction of high quality bespoke gene libraries with specific ratio-control at mutation sites. (businesswire.com)
  • cellular
  • For cell populations with bimodal gene expression, this difference is particularly pronounced, and may involve stochastic transitions between two cellular states that form distinct sub-populations. (nih.gov)
  • We found that knowing the fitness and nongenetic (cellular) memory associated with specific gene expression states were necessary for predicting the overall fitness of cell populations in combined environments. (nih.gov)
  • Agents that activate expression of specific genes to probe cellular pathways or alleviate disease would go beyond existing approaches for controlling gene expression. (jbsdonline.com)
  • single gene
  • This is usually used when you want just a short sequence from a single gene that you want to use for something such as transfecting into cells in vitro. (physicsforums.com)
  • Length
  • In order to optimize manufacturing and delivery time, synthetic genes ≤5 kb in length will be provided in a "best-fit" IDT cloning vector. (idtdna.com)
  • codons
  • The wild-type genes were recoded so that all codons represented highly used human codons. (umbc.edu)
  • Only about 10 codons in each recoded gene were not fully optimized because they doubled as restriction sites. (umbc.edu)
  • 2) then five additional Arg codons (at codon position 119,155,157,158 and 159) were optimized (M2 gene, CAI = 0.278) using oligodeoxynucleotide mutagenesis. (umbc.edu)
  • Libraries
  • We are now interested in further optimising the technology for high throughput, automated manufacture of whole gene libraries and we believe that this could represent a powerful and valuable tool for commercialisation in the synthetic biology arena" said Kevin Mathews, CEO of Isogenica. (businesswire.com)
  • sequence
  • We are currently awaiting correspondence from the author about the optimized gene sequence. (umbc.edu)
  • That site you linked to refers to a research tool where instead of cloning genes, you can have a DNA sequence synthesized. (physicsforums.com)
  • Laboratory
  • There has been a lot of clinical data recently suggesting that if you can stimulate the immune system in the right way you can get it to recognize cancer,' said Lu, who is head of the Synthetic Biology Group in MIT's Research Laboratory of Electronics. (genengnews.com)
  • vector
  • What is the standard delivery vector IDT uses for Genes and MiniGene™ Synthetic Genes? (idtdna.com)
  • The four chimeric magainin genes were subsequently ligated into a binary vector for Agrobacterium-mediated transformation. (lincoln.ac.nz)
  • Cloning of synthetic gene including antigens against Urinary Tract Infections in pET28a+ vector', Journal of Pharmaceutical & Health Sciences , 5(3), pp. 245-249. (jphs.ir)
  • The aim of this study is cloning of synthetic gene include antigens against UTIs in pET28a+ vector. (jphs.ir)
  • Gene and pET28a+ vector was checked by SnappGene. (jphs.ir)
  • Synthetic gene was multiplied by PCR and cloned in pET28a+ vector. (jphs.ir)
  • cells
  • To do this, Lu and a team including MIT postdocs Lior Nissim, Ph.D., and Ming-Ru Wu, M.D., Ph.D., have built a gene circuit encoded in DNA designed to distinguish cancer cells from noncancer cells. (genengnews.com)
  • Cancer cells differ from normal cells in the profile of their gene expression. (genengnews.com)
  • Yet, recently it became clear that cells with identical genomes exposed to the same environment can differ dramatically from the population average in their gene expression and division rate (individual fitness). (nih.gov)
  • We designed a closed-loop gene circuit that enables engineered cells to monitor increased thyroid hormone levels and drive the expression of a validated TSH receptor antagonist that competes with TSH as well as TSH receptor autoantibodies, thereby overcoming the pathophysiological activation of the thyroid gland. (pnas.org)
  • The synthetic version of Oct4 was much more efficient in turning on genes that instruct cells on how to be stem cells and, as a result, the cells did not need as much Oct4 for either reprogramming or to remain as stem cells thereby eliminating problems caused by too much Oct4. (bio-medicine.org)
  • In fact, the synthetic Oct4 could support stem cells under conditions that they do not normally grow. (bio-medicine.org)
  • The study showed that Oct4 was mainly responsible for turning on genes that instruct cells on how to become stem cells, rather than turning off genes that encourage the cells to differentiate. (bio-medicine.org)
  • The modified gene was either introduced into stem cells or used to reprogram adult skin cells. (bio-medicine.org)
  • Assembly
  • CAMBRIDGE, England--( BUSINESS WIRE )--Today, Monday 30th June, 2014, Isogenica, Cambridge UK and Imperial College London secure funding from the Technology Strategy Board (TSB), the UK's innovation agency, for a £347,000 project to optimise a gene library assembly technology for potential use in synthetic biology. (businesswire.com)
  • Dr. Baldwin, a Reader in the Life Sciences Department has developed foundational tools for synthetic biology including DNA assembly and he also has research interests in directed evolution. (businesswire.com)
  • thus
  • Thus, we have developed a method to more rationally design and construct synthetic GRNs using the framework of a modular DNA computer RTRACS. (nii.ac.jp)
  • construction
  • The synthetic genes selected for construction were based on the antimicrobial peptide magainin II. (lincoln.ac.nz)
  • The result demonstrated that the design, construction and transformation of synthetic antimicrobial magainin genes was a successful strategy in introducing a novel form of disease resistance in potato plants. (lincoln.ac.nz)
  • natural
  • The natural gene was only transformed in S. cerevisiae. (umbc.edu)
  • The yield in P. pastoris was 2 fold more than the production of the natural gene in C. rugosa. (umbc.edu)
  • Natural gene was not used in the experiment. (umbc.edu)
  • The natural gene produced sub-optimal concentration of hG-CSF when inserted into E. coli. (umbc.edu)
  • time
  • Stochastic gene expression at any instant of time can be described by a distribution (gray bars). (nih.gov)
  • Ten time course simulations of stochastic gene expression in bistable cell lineages are shown as illustration (gray traces). (nih.gov)
  • virus
  • Our synthetic biology pipeline for rapid sensor design and prototyping has tremendous potential for application for the Zika virus and other public health threats, enabling us to rapidly develop new diagnostics when and where they are needed most. (innovations-report.com)