• For example, studies suggest that ccRCC is closely associated with tumor suppressor von-Hippel Lindau ( VHL ) gene mutations that lead to stabilization of hypoxia inducible factors (HIF-1 α and HIF-2 α , also known as HIF1A and EPAS1) in both sporadic and familial forms [ 5 , 6 ]. (hindawi.com)
  • Site-directed mutagenesis (SDM) is a commonly used technique for introducing mutations into a gene of interest. (genengnews.com)
  • Here we describe the use of New England Biolabs' Gibson Assembly Master Mix in two different mutagenesis experiments: first, multiple mutations of the lacZ gene ( Figure 1 ) and second, the mutation of 5 of the 6 nucleotides at position 174-179 of eGFP from CTGACC to TTCTAT in order to change the amino acid sequence from LeuThr to PheTyr ( Figure 2 ). (genengnews.com)
  • In this experiment, multiple primers were designed to incorporate three mutations within the gene. (genengnews.com)
  • In this example, multiple mutations are introduced into the lacZ gene through overlapping primers followed by PCR. (genengnews.com)
  • For over a century, mutations in the yellow gene, named for its effects on pigmentation, have been known to reduce male mating success. (elifesciences.org)
  • Geneticists have long suggested that mutations in insect pigment genes cause changes in the fly's brain because these pigments are made from dopamine, a chemical messenger that acts in the brain. (elifesciences.org)
  • For many questions in biology, we want to know how different genes interact, and for this we need to introduce mutations into multiple genes," says Rudolf -Jaenisch, a biologist at the Whitehead Institute in Cambridge Massachusetts. (technologyreview.com)
  • Mutations in the MT-ND1 , MT-ND4 , MT-ND4L , or MT-ND6 gene can cause LHON. (medlineplus.gov)
  • Mutations in any of the genes disrupt this process. (medlineplus.gov)
  • Whole genome sequencing can also provide insights into the specific genes or mutations that may be linked to antibiotic resistance. (folkhalsomyndigheten.se)
  • Living systems contain thousands of genes and proteins, many of which interact in subtle ways. (networkworld.com)
  • DNA microarrays make it possible to analyze the mRNA expression of thousands of genes simultaneously. (karger.com)
  • Microarrays are one such tool that allows for the study of expression of thousands of genes simultaneously. (lu.se)
  • dCas9 can be fused to protein tags or the effector domains of transcription factors (proteins that bind DNA in promoter regions and simultaneously bind other regulatory proteins via effector domains, which can activate or repress transcription and thereby affect gene expression) to develop customized DNA binding proteins. (jax.org)
  • In addition, a PPI network can be modeled as an undirected graph, where vertices represent proteins and edges represent interactions between proteins, to prioritize disease associated genes or pathways and to understand the modus operandi of disease mechanisms [ 9 , 10 ]. (hindawi.com)
  • Positive and negative control serum specimens are run simultaneously to allow identification of viral proteins. (cdc.gov)
  • Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)
  • 1%) of genes are imprinted, meaning that gene expression occurs from only one allele (some recent studies have questioned this assertion, claiming that the number of regions of parent-of-origin methylation in, for example, the human genome, is much larger than previously thought). (stackexchange.com)
  • A new gene regulation and labeling platform dubbed "Casilio" can simultaneously execute distinct functions at multiple areas of the genome at the same time, expanding researchers' ability to study gene function and chromosome structure, Jackson Laboratory (JAX) researchers report. (jax.org)
  • The widely used CRISPR-Cas9 gene-editing technology has enabled researchers to alter genome sequence or gene expression with great precision, says JAX Assistant Professor Albert Cheng, Ph.D., first and co-corresponding author of a paper published in Cell Research . (jax.org)
  • Casilio 'orchestrates' variations throughout the genome, simultaneously and in high fidelity. (jax.org)
  • New technologies have emerged to translate the human genome sequence into gene function and improved diagnostics or treatment modalities. (karger.com)
  • The validation and prioritization of genes emerging from genome screening analyses in large series of clinical tumors has become a new bottleneck in research. (karger.com)
  • Indeed, a number of genes identified as potential manipulators of the host cell phenotype are absent from the T . equi genome. (biomedcentral.com)
  • To elucidate whether epigenetic effects play a role for these gene expression changes, we performed whole genome bisulphite sequencing to assess DNA methylation patterns in the lungs 56 days after exposure to MWCNTs. (cdc.gov)
  • Achieving systematic, genome-scale perturbations within intact biological systems is important for elucidating gene function and epigenetic regulation. (cdc.gov)
  • The objective of this work is to identify dysregulated genes and pathways of ccRCC temporally according to systematic tracking of the dysregulated modules of reweighted Protein-Protein Interaction (PPI) networks. (hindawi.com)
  • Finally, gene compositions of altered modules were analyzed, and pathways enrichment analyses of genes in altered modules were carried out based on Expression Analysis Systematic Explored (EASE) test. (hindawi.com)
  • We successfully identified dysregulated genes and pathways of ccRCC in different stages, and these might be potential biological markers and processes for treatment and etiology mechanism in ccRCC. (hindawi.com)
  • Delineating the pathogenesis of ccRCC by investigating the gene and epigenetic changes and their effects on key molecules and their respective biologic pathways is of crucial importance for the improvement of current diagnostics, prognostics, and drug development [ 4 ]. (hindawi.com)
  • The resulting comprehensive gene expression surveys lead to the identification of new genes and pathways with importance in cancer development and progression, or as targets for new therapies. (karger.com)
  • Simultaneously, innate immunity-related transcripts (Plunc, Bpifb1, Reg3g) and cancer-related pathways were downregulated. (cdc.gov)
  • IPA analysis of the differentially expressed genes in the whole blood suggested increased hematopoiesis, predicted activation of cancer/tumor development pathways, and atopy. (cdc.gov)
  • Two of these genes, arginase 1 and eotaxin 1 are the focus of continuing investigations in mouse models of asthma regarding differences in mechanistic pathways depending on the allergen. (cdc.gov)
  • Array studies provide candidate genes to identify common mechanistic pathways in the effector phase, as well as mechanistic pathways unique to individual allergens. (cdc.gov)
  • We observe sex-biased isoform switching consistently across development, but mostly in genes that are already differentially expressed. (lu.se)
  • Importantly, the interactions between genetic factors and environmental factors, termed as Gene-Environment Interactions or GxE, are believed to have effects on BMI. (ukbiobank.ac.uk)
  • We propose to develop a novel approach that can simultaneously predict, identify important risk factors, and study the joint effects of GxE and genetic factors. (ukbiobank.ac.uk)
  • Many diseases involve variations in gene expression rather than genetic sequence. (jax.org)
  • Yet campaigners persuaded EU regulators to lump gene-editing in with a ban on the rather different concept of genetically modified organisms (GMOs) where genetic sequences are moved between species. (farmersguardian.com)
  • The main contribution of this study is to establish an embedded multi-gene genetic programming (EMGGP) model for improved prediction of air demand. (iwaponline.com)
  • An embedded multi-gene genetic programming is developed. (iwaponline.com)
  • This unexpected synergy between gene variants demonstrated how even subtle genetic variations can impact neuronal function," said Brennand. (nih.gov)
  • Many of the genes affected downstream contained variants that had been linked to autism spectrum disorder or bipolar disorder , in addition to schizophrenia - consistent with other research suggesting genetic overlap across mental disorders. (nih.gov)
  • Precise and easy ways to rewrite human genes could finally provide the tools that researchers need to understand and cure some of our most deadly genetic diseases. (technologyreview.com)
  • Along with a technique called TALENs, invented several years ago, and a slightly older predecessor based on molecules called zinc finger nucleases, CRISPR could make gene therapies more broadly applicable, providing remedies for simple genetic disorders like sickle-cell anemia and eventually even leading to cures for more complex diseases involving multiple genes. (technologyreview.com)
  • Most conventional gene therapies crudely place new genetic material at a random location in the cell and can only add a gene. (technologyreview.com)
  • In particular, it gives scientists the ability to quickly and simultaneously make multiple genetic changes to a cell. (technologyreview.com)
  • This is not the same as genetic engineering using gene drive methods, which were developed to spread a genetic mutation through the population. (europeanscientist.com)
  • The team at TGen used Affymetrix Human Mapping 500K Arrays to simultaneously analyze 500,000 genetic markers from the people who were tested. (news-medical.net)
  • Genetic analysis of the haemagglutinin gene revealed multiple introductions of influenza viruses into Lebanon from different geographic sources during each season. (who.int)
  • We observed a downregulation in the expression of genes involved in host response, such as TNF signaling, IL-1β production, and phagosome formation in Mφ, and phosphatidylinositol signaling and TNF signaling in PMNs, being in line with the ability of the pathogen to survive within phagocytes. (frontiersin.org)
  • These developmental differences are caused by differential expression of genes and isoforms. (lu.se)
  • In contrast, sex-bias in pupae and adults is driven by the differential expression of genes. (lu.se)
  • In addition, long-read sequencing of one representative XDR ST235 isolate identified an integron carrying multiple resistance genes (including bla VIM-2), with differences in gene composition and synteny from the P. aeruginosa class 1 integrons described previously. (who.int)
  • Expected and potentially novel resistance genes are enriched in the top hits and are validated using individual sgRNA as well as cDNA overexpression. (cdc.gov)
  • We identified 24 common genes that were significantly altered in both PMNs and PBMCs, including genes involved in IFN signaling that were downregulated after treatment in both cell populations, and IL1R1 that was upregulated. (frontiersin.org)
  • Gene composition analyses of altered modules revealed that there were 56 common genes (such as MAPK1 , CCNA2 , and GSTM3 ) existing in the four stages. (hindawi.com)
  • 14 ] combined PPI and gene expression data to construct tissue-specific PPI networks for 60 tissues and used them to prioritize disease genes. (hindawi.com)
  • Many human illnesses, including heart disease, diabetes, and assorted neurological conditions, are affected by numerous variants in both disease genes and normal genes. (technologyreview.com)
  • Therefore, we have recently developed the tissue microarray (TMA) technology to efficiently test the clinical relevance of candidate genes. (karger.com)
  • DNA microarrays and TMAs provide a powerful approach to identify large numbers of new candidate genes, and rapidly validate their clinical impact in large series of human tumors. (karger.com)
  • Different statistical methods with varying selection criteria resulted in identification of similar candidate genes. (cdc.gov)
  • Array data are intended to provide candidate genes for hypothesis generation and further experimentation. (cdc.gov)
  • The reports on base sequences of spider silk protein genes have gained importance as the mechanical properties of silk fibers have been revealed. (bioone.org)
  • Comparative genomic analysis of T . equi revealed the phylogenetic positioning relative to seven apicomplexan parasites using deduced amino acid sequences from 150 genes placed it as a sister taxon to Theileria spp . (biomedcentral.com)
  • T . equi has lost the putative genes for host cell transformation, or the genes were acquired by T . parva and T . annulata after divergence from T . equi . (biomedcentral.com)
  • Micro- array data from the ovalbumin and TMA model of asthma were also compared to previous data using Aspergillus as allergen to identify putative asthma `signature genes', i.e. genes up-regulated with all 3 allergens. (cdc.gov)
  • These interactions emphasize the importance of considering the complex nature of schizophrenia and other psychiatric disorders, where a combination of gene variants contributes to disease. (nih.gov)
  • There is an overwhelming need for future studies to similarly model such multi-gene interactions in complex cells and circuits. (nih.gov)
  • The molecular interactions of genes and gene products underlie fundamental questions of biology. (lu.se)
  • The effect of expressing a gene is also influenced by which other genes are simultaneously expressed (functional interactions). (lu.se)
  • We find relatively little sex-bias in embryos and larvae at the gene level, but several sub-networks show sex-biased functional interactions in early developmental stages. (lu.se)
  • For example, it can be fused to domains that activate or repress gene expression, allowing researchers to increase or decrease gene expression at will without changing the actual sequence. (jax.org)
  • Single-cell RNA-sequence analyses demonstrate the homogenous distribution of the gene expression profile in individual cytomegalovirus-specific cytotoxic T-cells within each recipient. (nature.com)
  • On the other hand, bulk RNA-sequence analyses reveal that gene expression profiles among patients are different according to the cytomegalovirus reactivation pattern, and are associated with cytokine production or cell division. (nature.com)
  • This technology could allow researchers to perform microsurgery on genes, precisely and easily changing a DNA sequence at exact locations on a chromosome. (technologyreview.com)
  • DNA microarrays exploit primary sequence data to measure transcript levels and detect sequence polymorphisms, for every gene, simultaneously. (medscape.com)
  • The method uses an internal control sequence (spike) which is amplified and sequenced simultaneously with the sample to facilitate the assessment of the bacterial content and to detect amplification-inhibiting factors in the sample that may lead to a negative result. (folkhalsomyndigheten.se)
  • Birmingham University researchers recently led an international wake-up call to regulators stressing that gene editing and other new technologies contribute to food security. (farmersguardian.com)
  • Besides classical gene regulation, miRNAs have emerged as post-transcriptional regulators of angiogenesis. (plos.org)
  • Researchers have discovered that gene expression regulators work together to raise an individual's risk of developing schizophrenia . (nih.gov)
  • One possible clue: more than 40% of the suspect chromosomal sites contain regulators, called expression quantitative trait loci, or eQTLs, that govern the expression of multiple genes. (nih.gov)
  • Individually, these gene regulators have a modest effect on the brain. (nih.gov)
  • Finally, we discover that sex-biased networks are enriched by genes specific to the Nasonia clade, and that those genes possess the topological properties of key regulators. (lu.se)
  • Are there epigenetic changes in specific immune genes of patients with PTSD that may predispose them to respiratory illness? (cdc.gov)
  • The primary focus of this project is to analyse the in vivo fu nction in C. elegans of the metallopeptidase, cysteine proteinase and activation associated secreted protein families and to identify the key genes in each family. (europa.eu)
  • This panel enables us to simultaneously analyse multiple genes in one test. (qmul.ac.uk)
  • Therefore comparative genomic analysis of T . equi was undertaken to: 1) identify genes contributing to immune evasion and persistence in equid hosts, 2) identify genes involved in PBMC infection biology and 3) define the phylogenetic position of T . equi relative to sequenced apicomplexan parasites. (biomedcentral.com)
  • There were several common upregulated genes between whole blood and lungs, important for adaptive immune responses: Cxcr1, Cd72, Sharpin, and Slc11a1. (cdc.gov)
  • We also synthesize a library consisting of 70,290 guides targeting all human RefSeq coding isoforms to screen for genes which, upon activation, confer resistance to a BRAF inhibitor. (cdc.gov)
  • Given the purported accuracy that is already game changing, but if we can do 100 or 200 then maybe we can do more than merely eliminate some simple single gene disorders. (lesswrong.com)
  • A disease caused by a single mutation, in a single gene, that involves only a single cell type. (technologyreview.com)
  • In most land snails, the switch between dextrality and sinistrality is controlled by a single gene, meaning that reversals are likely to occur frequently. (bioedonline.org)
  • We've found that a single gene can have major effects on speciation and adaptation simultaneously. (bioedonline.org)
  • Investigation of UTR Variants by Computational Approaches Reveal Their Functional Significance in PRKCI Gene Regulation. (nih.gov)
  • Sexual dimorphism requires regulation of gene expression in developing organisms. (lu.se)
  • Continued studies focused on genes showing minimal changes in the TMA-induced model but with clear up-regulation in the ovalbumin model. (cdc.gov)
  • FenoLogica Biosciences, a startup using high-throughput analysis and machine learning to identify the effects genes have on cell phenotypes. (sme.org)
  • A brief introduction to high throughput technologies for measuring and analyzing gene expression is given. (lu.se)
  • Several independent high-throughput methods for differential gene expression (including SAGE and differential display) may enable function annotation of sequenced genomes [ 2 ] . (medscape.com)
  • miRNAs comprise a large group of endogenous non-coding RNAs that can block mRNA translation or negatively regulate mRNA stability and thereby play a central role in regulating gene expression [10] , [11] . (plos.org)
  • The genes were selected because they were deemed most likely to confer disease risk by regulating gene expression. (nih.gov)
  • Using the bioinformatics tool MAGICTRICKS and reversible pharmacological inhibition, we found that primed genes required the transcriptional activities of NF-κB. (frontiersin.org)
  • We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)
  • The researchers used the model to take a closer look at the downstream molecular consequences of gene expression changes known to occur in schizophrenia, and compared them with changes seen in postmortem brains and similarly modeled neurons of people with the illness. (nih.gov)
  • Rather than examining single genes, NGS allows laboratorians to use molecular barcoding to test multiple genes simultaneously. (aacc.org)
  • Similarly, by using host gene microarrays, one can explore host response at the level of gene expression and provide a molecular description of the events that follow infection. (medscape.com)
  • By understanding the molecular details of this interaction, we can identify virulence-associated microbial genes and host-defense strategies and characterize the cues to which they respond and mechanisms by which they are regulated. (medscape.com)
  • The pace of gene discovery rapidly accelerates, but its potential for explaining life at the molecular level remains largely unrealized because our understanding of gene function lags increasingly far behind. (medscape.com)
  • His interests include Bordetella molecular pathogenesis and gene expression bioinformatics. (medscape.com)
  • But most cell functions are more complex than that-being regulated by multiple genes. (jax.org)
  • Working with JAX Distinguished Visiting Professor Haoyi Wang, Ph.D., and other colleagues, Cheng was looking for a way to regulate multiple genes in the mouse and other cell and animal models. (jax.org)
  • However, studying multiple diseases simultaneously makes it challenging to discern clearly the intricate underlying mechanisms. (hindawi.com)
  • This will be done by combinatorial RNAi, i.e. simultaneously interfering with the expression of multiple genes by RNAi. (europa.eu)
  • The researchers experimentally mimicked the interaction of multiple risk genes thought to contribute to schizophrenia. (nih.gov)
  • The 2007 Gene Shoemaker Near Earth Object Grants, totaling $34,500 (US), were awarded on 7 March to an international collection of amateur astronomers and researchers. (planetary.org)
  • Although some activists claim genetically altered crops are a direct threat to human health, researchers generally dismiss such fears: There is little evidence that transgenic genes, in and of themselves, are likely to be toxic or promote disease. (technologyreview.com)
  • In laboratory cultures, company researchers have already shown that CD34+ cells can take up the genes and use them to make their own supplies of ribozymes. (newscientist.com)
  • Researchers in Pennsylvania say they've successfully stimulated the production of a pain-blocking protein in mice by using a modified herpes virus to attach the appropriate genes onto the animals' DNA. (scienceblog.com)
  • Researchers from the Francis Crick Institute and the University of Kent used gene-editing technologies to create male-only and female-only mice litters, according to a study published in Nature Communications (1). (europeanscientist.com)
  • Researchers at the Translational Genomics Research Institute (TGen) have announced the discovery of a gene that plays a significant role in memory performance in humans. (news-medical.net)
  • The study details how researchers associated memory performance with a gene called Kibra in over 1,000 individuals --both young and old-- from Switzerland and Arizona. (news-medical.net)
  • The researchers then validated their discovery by replicating the Kibra gene finding in two separate and distinct groups of subjects. (news-medical.net)
  • Researchers are also investigating whether changes in additional genes contribute to the development of signs and symptoms. (medlineplus.gov)
  • Pumped up by genes from as many as a half a dozen other species, the plants repel moths and viruses, fight off fungus diseases, and produce seed with a shelf life beyond that of their nonengineered cousins. (technologyreview.com)
  • This advantage enables more efficient gene modulation and labeling of chromosomal loci in live cells. (jax.org)
  • The defect may be present at either of 2 different gene loci. (medscape.com)
  • As the sequencing and gene annotation projects of entire genomes of many species are headed towards completion (see, e.g. [13] ), massive mapping efforts in biology are now focused on how the genes interact. (lu.se)
  • This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. (nih.gov)
  • The new system, called Casilio, combines an altered version of CRISPR (CRISPR-dCas9) with the Pumilio RNA-binding protein system to enable much broader gene manipulation power. (jax.org)
  • In a second step we will apply this technology to investigate the function of an additional nematode specific gene family, i.e. transthyretin-like protein family. (europa.eu)
  • What does this gene/protein do? (cancerindex.org)
  • The genes associated with LHON each provide instructions for making a protein involved in normal mitochondrial function. (medlineplus.gov)
  • We previously established a direct single-cell analysis to simultaneously identify and quantify in vivo CMV-CTL clones after allo-HCT 9 , 10 . (nature.com)
  • Not only did the research team identify that the Kibra gene was associated with memory performance, but they also showed that the gene is turned on in the hippocampus, a brain region known to be critical to memory function. (news-medical.net)
  • We're excited about the chance to identify a gene that accounts for some of variation in normal human memory and to use this information in the discovery of promising new memory-enhancing treatments. (news-medical.net)
  • By monitoring microbial gene expression, one can predict the functions of uncharacterized genes, probe the physiologic adaptations made under various environmental conditions, identify virulence-associated genes, and test the effects of drugs. (medscape.com)
  • Host profiling might also identify gene expression signatures unique for each pathogen, thus providing a novel tool for diagnosis, prognosis, and clinical management of infectious disease. (medscape.com)
  • They used the gene editing tool CRISPR to simultaneously increase or decrease expression of four schizophrenia-implicated genes known to harbor eQTLs. (nih.gov)
  • After harvesting blood cell precursors called hematopoietic stem cells from the bone marrow of a sickle-cell patient, scientists would use CRISPR to correct the defective gene. (technologyreview.com)
  • Whole blood gene expression profiling is a promising new approach for the identification of novel disease biomarkers. (cdc.gov)
  • As with learning a language, the best way is to be immersed, and let the entire associative mess emerge simultaneously. (lesswrong.com)
  • A rapid stool assay for Shiga toxin or, when available, a test for the gene that encodes the toxin may be helpful. (msdmanuals.com)
  • Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
  • We used RNA sequencing coupled with chromatin immunoprecipitation sequencing to analyze primed microglial inflammatory gene expression and modifications to histone H3 lysine 4 trimethylation (H3K4me3) at the promoters of primed genes. (frontiersin.org)
  • An individual T-cell has a specific complementarity-determining region 3 (CDR3) of T cell receptor (TCR)-α and -β, which is a result of the recombination of somatic TCR V-(D)-J genes and junction diversity. (nature.com)
  • However, biologists do believe that in some cases foreign genes in crops can pass into other, nonagricultural species, with potentially dangerous effects. (technologyreview.com)
  • Results of the study showed that all observed traits showed the importance of both additive and dominance gene effects. (hindawi.com)
  • Notably, all of these gene changes resulted in loss-of-brain-function effects when screened one-at-a-time in a zebrafish model," said Brennand. (nih.gov)
  • Taken together, the present study revealed that MWCNTs exert epigenetic effects in the lungs of exposed animals, potentially driving the subsequent gene expression changes. (cdc.gov)
  • With this feature, Casilio can now conduct different functions at different genomic sites--e.g., upregulating one set of genes while repressing another set. (jax.org)
  • thus, hybrid sterility prevents the movement of genes from one species to the other, keeping both species distinct (Keeton 1980). (newworldencyclopedia.org)
  • Further research is needed, first to develop the particular gene-editing toolkits for different species, and then to check they are safe and effective. (europeanscientist.com)
  • A skewed preference for BV7 genes and sequential "G" amino acids motif is observed in complementarity-determining region-3 of T cell receptor-β. (nature.com)
  • The plants are transgenic-that is, genes from other organisms have been inserted into their chromosomes. (technologyreview.com)
  • Our analysis identified 50 genes that will be useful for definitive phylogenetic classification of T . equi and closely related organisms. (biomedcentral.com)
  • The main emphasis is on supervised machine learning methods for classification and prediction of tumor gene expression profiles. (lu.se)
  • The yellow flies had a mutation in one specific gene and these mutants did not only look different from normal flies, they behaved differently too. (elifesciences.org)
  • Since genes are composed of a given alphabet (A, T, C, G) with fixed pairing properties A-T and C-G, probes can be constructed that attract gene transcripts extracted from cell tissues and cultures. (lu.se)
  • These dCas9-activator fusions targeted to the promoter region of endogenous genes can then modulate gene expression 7 - 11 . (cdc.gov)
  • Here we show the features of repertoire diversity and the gene expression profile of HLA-A24 cytomegalovirus-specific cytotoxic T-cells in actual recipients according to the cytomegalovirus reactivation pattern. (nature.com)
  • Applications range from testing for specific genes to pinpointing disease types to monitoring treatments. (networkworld.com)
  • The relative value of general combining ability (GCA) was greater than specific combining ability (SCA) for number of pod clusters per plant, number of branches per plant, plant height, days to maturity, and 100-seed weight which indicated the importance of additive gene effect. (hindawi.com)
  • We asked if the whole blood transcriptome reflects pathology-specific changes in lung gene expression caused by MWCNT. (cdc.gov)
  • Our previous work has shown that industrially produced MWCNTs trigger specific changes in gene expression in the lungs of exposed animals. (cdc.gov)
  • There were mineral- and habit-specific patterns of gene expression dysregulation at s.a. equivalent doses. (cdc.gov)
  • Genes whose expression levels rise and fall similarly in a large set of samples, may be considered coexpressed. (mdpi.com)
  • This was many more than would be expected if the genes had been merely acting individually, suggesting an underlying mechanism that is synergistic rather than additive. (nih.gov)
  • As a result, the 10-year-old drugmaker is able to conduct 1,000 experiments with drug candidates simultaneously. (sme.org)
  • Gene coexpression analysis refers to the en masse discovery of coexpressed genes from a large variety of transcriptomic experiments. (mdpi.com)
  • In what follows we limit ourselves to the analysis of gene expression measurements obtained using microarrays with small spots of DNA fixed to glass slides. (lu.se)
  • Typically when data are presented, the fluor- escent intensity from the tissue is pseudo-colored red and the intensity from the reference green, and the logarithmic ratio of background corrected red and green intensities for each gene (spot) is subject to analysis (see Fig. 1 ). (lu.se)
  • Systematic interrogation of gene function requires the ability to perturb gene expression in a robust and generalizable manner. (cdc.gov)
  • for example, Chu and Chen [ 13 ] combined PPI and gene expression data to construct a cancer perturbed PPI network in cervical carcinoma to study gain- and loss-of-function genes as potential drug targets. (hindawi.com)
  • The study reveals the importance of scientists considering that genes that affect behavior may do so by changing anatomy rather than by altering the brain. (elifesciences.org)
  • We have added several of them to a list of genes worthy of further study for possible involvement in schizophrenia. (nih.gov)
  • Gene expression associated with PTSD in World Trade Center responders: An RNA sequencing study. (cdc.gov)
  • however, some strains have acquired genes that enable them to cause intestinal infection. (msdmanuals.com)
  • The signature of our top screening hits is significantly correlated with gene expression data from clinical melanoma samples. (cdc.gov)
  • In Chris Kraft's autobiography Flight: My Life in Mission Control , the former flight director and former director of Johnson Space Center cited Gene Kranz' decision to immediately isolate or seal off the surge tank as being one of the things that made rescuing the crew possible. (universetoday.com)
  • We, therefore, tested the hypothesis that hypoxia would enhance or "prime" microglial pro-inflammatory gene expression in response to a later inflammatory challenge without programmatically increasing basal levels of pro-inflammatory cytokine expression. (frontiersin.org)
  • Surprisingly, however, the majority of primed genes were not enriched for H3K4me3 acutely following hypoxia exposure. (frontiersin.org)