Loading...
  • Keio
  • These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. (psu.edu)
  • deletion mutant
  • The U L 47 deletion mutant exhibited reduced plaque size and more than 100-fold decrease in intracellular and extracellular viral titers in cultured cells. (asm.org)
  • There was no evidence for a change in immediate-early gene activator function of VP16 in the U L 47 deletion mutant virus-infected cells, since bovine ICP4 mRNA and protein levels were similar to those in the wild-type and revertant virus-infected cells throughout the course of infection. (asm.org)
  • Whereas VP16, glycoprotein C (gC), gB, and VP5 were expressed to wild-type levels in the U L 47 deletion mutant-infected cells, the gD and VP22 protein levels were significantly reduced. (asm.org)
  • Extracellular virions of the U L 47 deletion mutant contained reduced amounts of gD, gB, gC, and VP22 but similar amounts of VP16 compared to those of wild-type or revertant virus particles. (asm.org)
  • In addition, the U L 47 gene product was indispensable for BHV-1 replication in vivo, since no clinical manifestations or viral shedding were detected in the U L 47 deletion mutant-infected calves, and the virus failed to induce significant levels of humoral and cellular immunity. (asm.org)
  • inactivation
  • Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. (psu.edu)
  • found
  • Bo's lack of furnishings means that he has the ancestral form of that gene, matching the patterns found in wolves, according to the research. (go.com)
  • While the coat gene was initially found in Portuguese water dogs, researchers used genomes from 903 dogs in 80 different breeds to reach their overall conclusions. (go.com)
  • Additional lab studies found that melanoma cells with the ERBB4 defect were dependent on the mutant gene for their growth. (bio-medicine.org)
  • The researchers also found that two additional PTK genes -- FLT1 and PTK2B -- were mutated in about 10 percent of the tumor samples. (bio-medicine.org)
  • RNAi constructs targeting two of those genes were shown previously to extend life, and RNAi's targeting five novel genes were found here to increase lifespan. (nih.gov)
  • As expected, we found genes affecting cytokinesis and/or the actin cytoskeleton within this set ( ain1 , acp2 , imp2 ). (g3journal.org)
  • We finally found out who has and who doesn't have the gene that's screwed up my little girls chances at a life of not being afraid of the mildest friction. (blogspot.com)
  • characterization
  • Evidence for the indirect pathway in plants had initially come from a variety of biochemical studies, 18 O 2 -labeling experiments, and the characterization of ABA-deficient mutants. (plantphysiol.org)
  • Our understanding of the functions and synthesis of ABA has been greatly enhanced by the identification and characterization of ABA-deficient mutants (Table I ). The ABA-deficient mutants have been identified by the following phenotypes: precocious germination, susceptibility to wilting, an increase in stomatal conductance, and an ability to germinate and grow on media containing a high concentration of Suc or salt. (plantphysiol.org)
  • In the present report, we describe the characterization of U L 47 gene-deleted BHV-1 in cultured cells and its natural host. (asm.org)
  • resistance
  • Therefore, there are limitations in current treatments targeting a few cancer-inducing genes without further knowledge on gene networks, hence current drugs are only effective in a few patients and often induce drug resistance. (eurekalert.org)
  • Caenorhabditis elegans PI3K mutants reveal novel genes underlying exceptional stress resistance and lifespan. (nih.gov)
  • Two age-1 nonsense mutants, truncating the class-I phosphatidylinositol 3-kinase catalytic subunit (PI3K(CS)) before its kinase domain, confer extraordinary longevity and stress-resistance to Caenorhabditis elegans. (nih.gov)
  • Hooykaas, Paul 2004-10-19 00:00:00 Transgenic arabidopsis plants were isolated that contained a T-DNA construct in which the promoter of an auxin-inducible glutathione S-transferase (GST) gene from tobacco was fused to the kanamycin resistance (nptII) as well as to the β-glucuronidase (gusA) reporter gene. (deepdyve.com)
  • tumor
  • Investigating core mechanisms of how cancer cells respond to their surroundings in the human body, biologists at the University of California San Diego have discovered new evidence about mutant p53 that may reshape our understanding of tumor growth and ultimately how we treat cancer. (ucsd.edu)
  • Thus, rather than fighting tumor growth, mutant forms of p53 appear to be tapping into the body's immune response system to fuel pro-inflammatory responses that increase cancer growth. (ucsd.edu)
  • Led by Yardena Samuels of the National Human Genome Research Institute, the research team from the U.S. National Institutes of Health (NIH) sequenced the protein tyrosine kinase (PTK) gene family in tumor and blood samples from people with metastatic melanoma. (bio-medicine.org)
  • researchers
  • Researchers from the NIH and several universities have shown that variation among the coats of different dog breeds can be traced back to three genes. (go.com)
  • By studying dogs' coats -- a trait easily observed by the human eye -- researchers wanted to better understand how the genes affected the coats, leading, they hope, to more specific findings in the genome later on. (go.com)
  • Through large-scale genomic analyses, the researchers discovered that mutant p53 amplifies the impact of inflammation leading to increases in the invasive behavior of cancer. (ucsd.edu)
  • Researchers wanted to identify the genes that protect people with multiple risk factors from developing type 2 diabetes. (diabetes.org)
  • While SLC30A8 appears to be linked to diabetes, the researchers don't actually know what this gene does or how it's related to diabetes. (diabetes.org)
  • organism
  • For transfection experiments or other experiments where a gene from one organism or tissue is put into a system that is not its normal environment, the annotator should use the author's intent and interpretation of the experiment as a guide as to whether IMP or IDA is appropriate. (geneontology.org)
  • cassette
  • HYG-containing vectors functioned equivalently to those containing the neomycin phosphotransferase (NEO) cassette previously used for extrachromosomal transformation or gene targeting. (pnas.org)
  • protein
  • Tegument protein VP8 encoded by the U L 47 gene of bovine herpesvirus type 1 (BHV-1) is the most abundant constituent of mature virions. (asm.org)
  • And once you have identified what protein has been mutated how would you identify the gene? (biology-online.org)
  • siblings
  • In a family photo of the Walshes before they discovered the siblings shared a potentially fatal gene, are (from left) Steve Walsh, his sister Beth Lambert, brother Mike, sister Kathy Flores, brother Dave, and their mother, Mary Walsh. (bostonglobe.com)
  • A mutated gene is one you did NOT get from your parents, that you have and your siblings don't. (blogspot.com)
  • particular gene
  • A particular gene might determine whether your eyes are blue or black, but that same gene in another person won't give him stronger back muscles. (blogspot.com)
  • differences
  • The IMP evidence code covers those cases when the function, process or cellular localization of a gene product is inferred based on differences in the function, process, or cellular localization between two different alleles of the corresponding gene. (geneontology.org)
  • variation
  • While the three genes accounted for most of the variation, they showed up and combined a bit differently in the different breeds. (go.com)
  • lethal
  • Triple mutant for Dbp, Tef, Hlf cause lethal seizures, but showed normal circadian rhythm. (go.jp)
  • strain
  • Though vaccination with MtbΔmms strain induced protection in the lungs of guinea pigs, the mutant strain failed to control the hematogenous spread of the challenge strain to the spleen. (biologists.org)
  • In order to generate a strain that overcomes the pathology caused by MtbΔmms in spleen of guinea pigs and controls dissemination of the challenge strain, MtbΔmms was genetically modified by disrupting bioA gene to generate MtbΔmmsb strain. (biologists.org)
  • MtbΔmmsb mutant strain was highly attenuated for growth and virulence in guinea pigs. (biologists.org)
  • growth
  • In this study, it is hypothesized that the mechanical properties of the wall within the growth zone of stiff mutant sporangiophores are different compared to wild type (WT). (frontiersin.org)
  • Experimental results demonstrate that turgor pressure is larger but irreversible wall deformation rates within the growth zone and growth zone length are smaller for stiff mutant sporangiophores compared to WT. (frontiersin.org)
  • invasion
  • I bring it up because it's a little similar to the X-gene (and to the metagene that DC introduced in Invasion , and which I'm convinced was stolen from Wild Cards ), except they work the science out much more tightly. (blogspot.com)
  • Previously
  • This is quite exciting, progressing from the identification of the gene in 1991 to now believing we will be able to treat a previously untreatable condition. (meta-religion.com)
  • function
  • A study published in the journal Nature Communications led by Homa Rahnamoun in Shannon Lauberth's laboratory at UC San Diego's Division of Biological Sciences uncovered a new mechanism linking mutant p53 function to chronic inflammation-a long-term condition typically associated with a response in the body's immune system-which can be induced in situations ranging from stress to food consumption. (ucsd.edu)
  • If the experiments were conducted to assess the normal function of the gene and the assay system is believed to reproduce this function, i.e., the authors would consider their experiment to be a direct assay, and not a comparison between various alleles of a gene, then the IDA code should be used. (geneontology.org)
  • We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. (psu.edu)
  • human
  • Yet, mutant forms of p53 have been linked to more cases of human cancer than any other gene. (ucsd.edu)
  • The mutant human ND4 gene with a FLAG epitope tag followed by mitochondrial encoded mCherry under control of the mitochondrial promoter (sc-HSP-ND4G1019AmtmCherry) was packaged with mito-targeted (COX8) scAAV that was injected into fertilized oocytes using a standard pronuclear microinjection protocol. (arvojournals.org)
  • Pronuclear injection of mutant human mitochondrial genes is an innovative biotechnical advance for evaluating LHON and likely other devastating neurodegenerative disorders with mitochondrial etiology and their treatment. (arvojournals.org)
  • studies
  • Much of the recent work in characterizing these genes has confirmed previous biochemical studies. (plantphysiol.org)
  • To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. (psu.edu)
  • animals
  • Vaccination with Mtb Δ mmsb mutant generated significant protection in comparison to sham-immunized animals at 4 and 12 weeks post-infection in lungs and spleen of infected animals. (biologists.org)
  • Family
  • The PTK family includes many genes that, when mutated, promote many types of cancer, including brain, gastric and lung malignancies, according to background information provided in the news release. (bio-medicine.org)
  • It features new articles from a number of familiar people in the anti-race crowd specifically commenting on Leroi's March pro-race essay, A Family Tree in Every Gene in the New York Times . (gnxp.com)
  • And if their "mutant" gene connects them to their family rather than dividing from their family, then the power of the metaphor is severely weakened. (blogspot.com)
  • And if "mutants" share a specific gene, then it seriously weakens the idea of the X-Men as a family of choice. (blogspot.com)
  • cancer
  • He continued, "In this research, a systems biology approach identified the principle of gene networks for the first time to suggest the possibility of anti-cancer drug target identification from a new perspective. (eurekalert.org)