Genes in normoxia. Medical search. Frequent questions

The hypoxia-sensitive striped bass ( Morone saxatilis) and the hypoxia-tolerant Nile tilapia ( Oreochromis niloticus) were sampled at four key time points: 1) prior to hypoxia exposure (normoxic control), 2) after four hours at the species' specific Pcrit (hypoxia), 3) immediately upon return to normoxia (reperfusion), and 4) after four hours at normoxia (recovery). (georgiasouthern.edu)

Human adult mobilized CD34+ cells were cultured for 21 days under normoxia (21% O 2 ) or hypoxia (2% O 2 ) in vessels coated with optimized concentrations of Delta1. (confex.com)
MATERIALS AND METHODS: NC and inner annulus chondrocyte-like cells (CLC) from immature pigtails (freshly isolated cells/tissue, 3D-alginate beads, 3D-clusters) were cultured for up to 16 days under normoxia and hypoxia. (ox.ac.uk)

In normoxia, HIF alpha subunits are marked for the ubiquitin-proteasome degradation pathway through hydroxylation of proline-564 and proline-402 by PHD2. (wikipedia.org)
PHDs hydroxylate conserved prolyl residues from the HIF-1 and HIF-2 subunits, hence marketing their binding towards the von Hippel Lindau (VHL) tumor suppressor proteins, which goals HIF isoforms for proteasomal degradation in normoxia (21C24). (bioskinrevive.com)
Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. (icr.ac.uk)
LIMD1 complexes with PHD2 and VHL in physiological oxygen levels (normoxia) to facilitate proteasomal degradation of the HIF-α subunit. (york.ac.uk)
Here, we identify LIMD1 as a HIF-1 target gene, which mediates a previously uncharacterised, negative regulatory feedback mechanism for hypoxic HIF-α degradation by modulating PHD2-LIMD1-VHL complex formation. (york.ac.uk)
Hypoxic induction of LIMD1 expression results in increased HIF-α protein degradation, inhibiting HIF-1 target gene expression, tumour growth and vascularisation. (york.ac.uk)
To minimize side effects, we designed a hypoxia-inducible CAR (HiCAR), which is driven by a hypoxia response element (HRE), and consists of a conventional CAR and an oxygen-dependent degradation domain (ODD) that is actively degraded under normoxia but stabilized under hypoxia. (biomedcentral.com)

Cells will adapt by activating the expression of genes involved in metabolic adaptation, such as those involved in glycolysis. (bmj.com)
Note that activation of these specific genes may not result in identical metabolic consequences to that of other related family members that are frequently mutated in cancer (e.g. (harvard.edu)

HIF-1α is a ubiquitous, constitutively synthesized transcription factor responsible for upregulating the expression of genes involved in the cellular response to hypoxia. (wikipedia.org)
The project will identify key genes, pathways, and biological processes that are differentially regulated in response to hypoxia. (lu.se)
In order to gain insights into the role of Net in hypoxia, we have analysed in parallel the genes regulated by HIF-1alpha, the classical factor involved in the response to hypoxia. (gsea-msigdb.org)

Results: In BEAS-2B and hAELVi, hypoxia significantly dowregulated genes related to fibrosis, mitochondrial stress, oxidative stress, apoptosis and inflammation whereas VEGF receptor 2 increased. (lu.se)
This compound markedly decreased the hypoxia-induced accumulation of HIF-1α protein dose-dependently, whereas it did not affect the expressions of HIF-1β and topoisomerase-I (topo‑I). Furthermore, celastrol prevented hypoxia-induced expression of HIF-1 target genes for vascular endothelial growth factor (VEGF) and erythropoietin (EPO). (spandidos-publications.com)
The concentrations of growth factors (such as HGF, bFGF, and VEGF) in the cell culture supernatants collected from culture media of the hypoxia-preconditioned AT-MSCs and UC-MSCs (as well as those from AT-MSCs and UC-MSCs cultured in normoxia) were measured by ELISA. (minervamedica.it)
For AT-MSCs, there was a significant increase in VEGF and bFGF production when preconditioned with hypoxia, compared to normoxia, while HGF production was only slightly increased. (minervamedica.it)
Up-regulation of VEGF gene expression by hypoxia was markedly suppressed by a TRPC channel blocker. (interleukinreceptor.com)
A concentração protéica de VEGF e dos genes das isoformas VEGFA, VEGFB e VEGFC, assim como do IFN? (unicamp.br)

Alternating periods of hypoxia and normoxia in the tumor support the in behalf ofemost-rate of tumor cells with elevated mutation frequency with a more accentuation intractable and aggressive phenoparadigm. (ika.ie)
HiCAR-T cells showed enhanced cytotoxicity against tumor cells under hypoxia compared to normoxia in vitro and antitumor efficacy comparable to that of conventional CAR-T cells in vivo. (biomedcentral.com)

These genes increase oxygen availability by inducing erythropoiesis and angiogenesis, and also reduce its demand via regulation of energy metabolism by reducing oxidative phosphorylation and inducing non-oxygen-demanding glycolysis [ 5 , 6 , 7 ]. (springer.com)
The project aims to elucidate the impact of oxygen levels on the HIF-2α pathway and its target genes in the context of intestinal stemness and tissue renewal. (lu.se)
Genes up-regulated in SEND cells (skin endothelium) at normal oxygen (normoxia) conditions after knockdown of HIF1A [GeneID=3091] by RNAi. (gsea-msigdb.org)
The purpose of this review is to summarize the known information on the physiological response of organisms exposed to high molecular oxygen concentrations, especially the effect on growth, metabolism, enzyme activity, protein oxidation, and gene expression. (biomedcentral.com)

To assess the relative impact of normoxia and hypoxia on the HSC compartment, we performed gene set enrichment analysis (GSEA) of cells within HSC cluster 5 from each culture condition. (confex.com)
Moreover, utilizing advanced bioinformatics tools, the project will employ Gene Set Enrichment Analysis (GSEA) to discern the enrichment patterns of HIF-2α target upregulated genes within the obtained datasets. (lu.se)
4) Also, we will trying to integrate the results and information of hypoxia-induced gene expression profiles, epigenetic regulation, and the embryo development signaling to identify the mechanisms of the influences of hypoxia in embryo development by the genome-wide iterative enrichment analysis in composite regulatory signature database (CRSD). (tmu.edu.tw)

A PHD2 knockdown showed increased levels of HIF-1α under normoxia, and an increase in HIF-1α nuclear accumulation and HIF-dependent transcription. (wikipedia.org)
In this regard, we performed RNA interference to knockdown COX-2 gene in vitro and immunohistochemistry to evaluate the protein expression of COX-2 and CA-IX in human colon cancer tissue specimens ex vivo. (unibo.it)

The ternary complex factor Net/Elk3 is downregulated in hypoxia and participates in the induction by hypoxia of several genes, including c-fos, vascular endothelial growth factor and egr-1. (gsea-msigdb.org)

this association is primarily based on the HIF-1-mediated regulation of genes that play pivotal roles in the central features of cancer pathogenesis such as angiogenesis, invasion, metastasis and anti-apoptosis. (spandidos-publications.com)
Most important to gliomagenesis, HIF-1 is a potent activator of angiogenesis and invasion through its upregulation of target genes critical for these functions. (northwestern.edu)
EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. (icr.ac.uk)

These gene products may include proteins such as glycolytic enzymes and angiogenic growth factors. (wikipedia.org)

Some of them are the protein-coding genes for RNA polymerase subunits, ribosomal proteins, enzymes of lipid metabolism, and many other enzymes. (blogspot.com)

mRNA expression of genes and proteins related to disease pathology were. (lu.se)
Methods used were metastatic invasion assay, gene expression analysis by RT-PCR on hypoxic response and oncopathway genes, and large-scale protein profiling to detect proteomic levels of metalloproteinases and surface proteins known to be involved in colorectal cancer invasion. (dynamics365commerce.ms)

A total of 10,318 mRNAs were identified to be expressed in twenty libraries (five timepoints), and 3,198 differentially expressed mRNAs (DE mRNAs) were detected at five timepoints (hypoxia vs. normoxia). (figshare.com)

The ability to knock out human genes using CRISPR technology has opened to door to testing for essential genes in tissue culture cells. (blogspot.com)
These are the genes activated by heat shock and other forms of stress and they churn out a lot of HSP70 chaperone in order to save the cells. (blogspot.com)
This is an example of the kind of gene that will be missed in the screen because the cells were not stressed during the screening. (blogspot.com)
At optimal coating densities of Delta1, the frequency of LTR-HSCs measured by limiting dilution analysis 16 weeks after transplantation into NSG mice was 4.9- and 4.2-fold higher in hypoxic cultures (1 in 1,586 CD34+ cells) compared with uncultured cells (1 in 7,706) and the normoxia group (1 in 5,090), respectively. (confex.com)
To corroborate these findings molecularly, we performed transcriptomic analyses and found significant upregulation of a distinct HSC gene expression signature in cells cultured with Delta1 in hypoxia (Fig. A). Collectively, these data show that hypoxia supports a superior ex vivo expansion of human HSCs with LTR activity compared with normoxia at optimized densities of Delta1. (confex.com)
Protein-expression was analysed by immunohistology and gene-expression analysis was carried out on freshly isolated cells and cultured cells. (ox.ac.uk)
RT-PCR showed that U-87 MG cells expressed four TRPC isoforms in normoxia: TRPC1, 3, 4, and 5. (interleukinreceptor.com)
We applied a range of molecular and cellular techniques to measure cell survival, cell cycle progression, protein expression and DNA damage combined with a transcriptomic micro-array approach in D283-MED cells, for global gene expression analysis in acute and chronic hypoxic conditions. (biomedcentral.com)
Using transcriptomic analysis in D283-MED cells, we found a large transcriptional remodelling upon long term hypoxia, in particular the expression of a number of genes involved in detection and repair of double strand breaks (DSB) was altered. (biomedcentral.com)
This investigation was prompted by the observation that in colorectal cancer cells the expression of COX-2 protein is positively correlated with that of the hypoxia survival gene Carbonic Anhydrase-IX (CA-IX). (unibo.it)
Experimental Design: Since COX-2 gene expression and activity is increased in hypoxia, and that CA-IX is expressed also in normoxia in colorectal cancer cells, we tested the hypothesis that COX-2 activity in normoxia, as well as in hypoxia may be functionally linked to that of CA-IX gene. (unibo.it)
Cells were harvested using Accutase solution after 24 hours of normoxia, hypoxia and hyp oxia with bevacizumab treatment. (vegfrinhibitors.com)
27 samples consisting of MCF7 human breast cancer cells treated with 6 μM BIX-01294 in 0.1% dimethyl sulfoxide (DMSO) or 0.1% DMSO carrier controls and untreated controls in acute (4 hours) hypoxia, chronic (24 hours) hypoxia (1% O2) or normoxia (21% O2) control conditions in triplicate cultures. (nih.gov)
Glutamine-driven oxidative phosphorylation is a major ATP source in transformed mammalian cells in both normoxia and hypoxia. (harvard.edu)

Hypoxia-inducible factor prolyl hydroxylase 2 (HIF-PH2), or prolyl hydroxylase domain-containing protein 2 (PHD2), is an enzyme encoded by the EGLN1 gene. (wikipedia.org)

What they should detect is all of the genes required in core metabolism. (blogspot.com)
In P. leucopus, alterations in genes related to cholesterol metabolism were also identified. (nsf.gov)
secondary endpoints were as follows: changes in body weight, waist circumference, blood pressure, cardiac dimensions, and adipose and muscle tissue metabolism and gene expression. (dlr.de)

The HSP70 gene family encodes the major heat shock protein of molecular weight 70,000. (blogspot.com)
To detect apoptosis at the molecular level, RT-qPCR will be used to quantify changes in the transcriptome of cardiomyocytes of pro-apoptotic ( BAX and FASL ), anti-apoptotic ( BCL2 and FLIP ) and repair ( HSP70 ) genes. (georgiasouthern.edu)

Interestingly, in hypoxia, Net regulates the expression of several genes known to control HIF-1alpha stability, including PHD2, PHD3 and Siah2, suggesting that Net regulates the stability of HIF-1alpha. (gsea-msigdb.org)

Epigenetic regulation is very important in early embryo development for it can affect genomic imprinting, gene reprogramming and gene expression. (tmu.edu.tw)

By performing unbiased analyses of the m1A, m5C, m6A and m7G methylation of mRNA, we found that within the whole brain transcriptome, with the increase of the gene expression levels, the overall level of each of these four modifications on the related genes was also progressively increased. (biomedcentral.com)

We observed only minimal basal expression under normoxia (5.1% and 43 for CD19-HiCAR, 8.6% and 42 for AXL-HiCAR, and 13.2% and 113 for HER2-HiCAR, respectively), and hypoxia induced a significant increase in CAR expression, with 70.2% and 601 for CD19-HiCAR, 61.9% and 307 for AXL-HiCAR, and 64.9% and 489 for HER2-HiCAR, respectively (Fig. 1 a-f). (biomedcentral.com)

We hypothesize that tilapia will have lower cellular markers of apoptosis due to higher anti-apoptotic and repair gene expression in their cardiomyocytes when compared to the striped bass, which we hypothesize will have higher cellular markers of apoptosis and pro-apoptotic gene expression levels. (georgiasouthern.edu)

Our customer needed to obtain in-vivo like responses to drug treatment, through monitoring parameters such as gene expression, biomarker analysis, cellular viability, invasion capability. (dynamics365commerce.ms)

Sandwalk: How many human protein-coding genes are essential for cell survival? (blogspot.com)
The human genome contains about 20,000 protein-coding genes and about 5,000 genes that specify functional RNAs. (blogspot.com)
Each group identified between 1500 and 2000 protein-coding genes that are essential in their chosen cell lines. (blogspot.com)
One of the annoying things about all three papers is that they use the words "gene" and "protein-coding gene" as synonyms. (blogspot.com)
The only genes they screened were protein-coding genes but the authors act as though that covers ALL genes. (blogspot.com)
2015). What they meant is that they represent 9.2% of protein-coding genes. (blogspot.com)
Among the genes that exhibited the most pronounced inversion in their coordination profiles during aging was THBS4, that encodes for thrombospondin-4, a protein that was recently identified as rejuvenation factor in mice. (nsf.gov)

It will extend to the identification and characterization of HIF-2α target genes, with a specific emphasis on their role in modulating the fundamental processes associated with intestinal stemness and tissue renewal. (lu.se)

The α subunit is stable in hypoxic conditions but is rapidly degraded in normoxia. (northwestern.edu)

We, therefore, tested the hypothesis that hypoxia would enhance or "prime" microglial pro-inflammatory gene expression in response to a later inflammatory challenge without programmatically increasing basal levels of pro-inflammatory cytokine expression. (frontiersin.org)
We used RNA sequencing coupled with chromatin immunoprecipitation sequencing to analyze primed microglial inflammatory gene expression and modifications to histone H3 lysine 4 trimethylation (H3K4me3) at the promoters of primed genes. (frontiersin.org)

Under hypoxia, the CDC proliferation rate increased by 1.4-fold, generating 6 × 10(6) CDCs with higher expression of cardiac stem cell and pluripotency gene markers compared to normoxia. (ox.ac.uk)

I hope it's just sloppy thinking when they say that their 1800 essential "genes" represent 9.2% of all genes in the genome (Wang et al. (blogspot.com)

Surprisingly, however, the majority of primed genes were not enriched for H3K4me3 acutely following hypoxia exposure. (frontiersin.org)
We found that both H3K4me3 and H3K36me3 globally decrease across all actively expressed genes with age. (nsf.gov)

In contrast, 29 unique significant and suggestive SNPs were found across growth period in the normoxia environment. (wur.nl)

Gene Ontology analysis in both species, for the genes that exhibited inverse transcriptomic coordination during aging pointed to alterations in the perception of smell, a known impairment occurring during aging. (nsf.gov)
Q-PCR and western blot analysis determined gene expressions. (bvsalud.org)
We have identified, in a large-scale analysis of RNA expression using microarrays, more than 370 genes that are regulated by Net in hypoxia. (gsea-msigdb.org)
Interestingly, whole transcriptome analysis revealed that a number of previously non-responsive genes become amenable to hypoxia following G9A inhibition, including many targets that drive cancer cell survival. (nih.gov)

I looked first at the genes encoding the enzymes for gluconeogenesis and glycolysis. (blogspot.com)

Here, there are at least four major and specific aims will be proceeding in three years in this project: (1) For the first year, the effects of hypoxia on embryo development and related gene expression (transcriptomics and microRNA) profiles will be examined by Affymetrix DNA Chip and Q-PCR-based megaplex pre-amp microRNA assay. (tmu.edu.tw)

Freshly isolated NC showed significantly higher KRT8 and CAIII but lower Col2 gene-expression than CLC. (ox.ac.uk)
Gene-expression of KRT8 and CAIII in NC fell significantly early in culture while Col2 did not decrease significantly within the culture period. (ox.ac.uk)
Postprandial energy expenditure was significantly increased under hypoxia, whereas activity energy expenditure was improved under normoxia. (dlr.de)

Using the bioinformatics tool MAGICTRICKS and reversible pharmacological inhibition, we found that primed genes required the transcriptional activities of NF-κB. (frontiersin.org)

However, it is still unknown whether hypoxia could affect the abundance of methylation modifications on mRNAs, which further affects the expression levels of these genes. (biomedcentral.com)

Surprisingly, when we compare the genes induced by hypoxia that require either Net or HIF-1alpha, the majority are the same (75%), suggesting that the functions of both factors are closely linked. (gsea-msigdb.org)

the red plots exemplify the genes in the lagging strand of M. bovis. (ika.ie)

Abstract Background Genes that belong to the same network are frequently co-expressed, but collectively, how the coordination of the whole transcriptome is perturbed during aging remains unclear. (nsf.gov)
To explore this, we calculated the correlation of each gene in the transcriptome with every other, in the brain of young and older outbred deer mice (P. leucopus and P. maniculatus). (nsf.gov)

We would like to know how many of those genes are essential for the survival of an individual and for long-term survival of the species. (blogspot.com)
Intriguingly, candidate genes rora, adamts3 and efna3a are reported related to hypoxia in other species. (wur.nl)

They are among the most highly conserved genes in all of biology and they are essential. (blogspot.com)

2 In addition, hypoxia induces genetic instability by the induction of fragile sites causing gene amplification. (bmj.com)

Adipose tissue triglyceride lipase, leptin, and hypoxia-inducible factor-alpha expression were increased by normoxia but not hypoxia. (dlr.de)

5′-GACGTG-3′) on the promoter region in various target genes ( 1 , 2 ). (spandidos-publications.com)
Upon stabilization or activation, HIF-1 translocates to the nucleus and induces transcription of its downstream target genes. (northwestern.edu)

On the other hand, therapeutic concentrating on of PHDs to induce a pseudohypoxic (activation of HIF and focus on genes in normoxia) condition is under energetic clinical advancement in the framework of anemia and various other diseases concerning hypoxia (33,34). (bioskinrevive.com)

I want to know about specific genes so I can see if the results conform to expectations. (blogspot.com)

They won't detect any of the genes required for spermatogenesis or embryonic development. (blogspot.com)

Using available RNA sequencing data from previously published studies on intestinal organoids, the project will compare the gene expression profiles with newly generated RNA sequencing data from our lab's organoid cultures at hypoxia. (lu.se)
Conclusions There sults suggest that aging is associated with more stringent retention of expression profiles for some genes and more abrupt changes in others, while more subtle but widespread changes in gene expression appear protective. (nsf.gov)

Anatomy37

Organisms12

Diseases25

Chemicals and Drugs70

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Psychiatry and Psychology1

Phenomena and Processes83

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena6

Health Care7

Upon return to normoxia1

Days under normoxia2

Degradation7

Metabolic2

Response to hypoxia3

VEGF6

Tumor2

Oxygen4

Enrichment3

Knockdown2

Vascular endoth1

Angiogenesis3

Angiogenic1

Subunits1

Proteins2

Differentially1

Cells14

EGLN11

Metabolism3

HSP702

PHD21

Regulation1

MRNA1

Hypoxia-induced1

Tilapia1

Cellular1

Protein7

Characterization1

Hypoxic conditions1

Inflammatory2

Markers1

Genome1

H3K4me32

Suggestive1

Analysis4

Enzymes1

Assay1

Significantly3

Inhibition1

Methylation1

Surprisingly1

Lagging strand1

Transcriptome2

Species2

Biology1

Induction1

Tissue1

Target2

Activation1

Specific1

Detect1

Expression profiles2