• Finally, genes encoding putative regulatory proteins such as extracellular function (ECF) sigma factors as well as small proteins had elevated expression levels in the presence of oxygen. (microbiologyresearch.org)
  • It can also identify putative haemin-independent H. haemolyticus strains and determine the serotype of typeable Haemophilus strains. (biomedcentral.com)
  • The annotation data was mined for gene products associated with virulence revealing a leukotoxin, haemolysin, filamentous haemagglutinnin, adhesin, hemin receptor, phage genes, CRISPR-associated proteins, ecotin and a putative type V secretion system. (westminster.ac.uk)
  • Data will be presented on comparative genomics of the three strains, with a focus on putative virulence genes. (westminster.ac.uk)
  • The expression of genes encoding formate uptake transporter (PG0209) and formate tetrahydrofolate ligase ( fhs ) was drastically elevated, which indicates that formate metabolism plays a major role under aerobic conditions. (microbiologyresearch.org)
  • In addition, metal transport was affected, and while iron-uptake genes such as the genes encoding the haemin uptake locus ( hmu ) were downregulated, expression of manganese transporter genes, such as feoB2 , was elevated in the presence of oxygen. (microbiologyresearch.org)
  • The cAMP-dependent pathway was found to be activated in K562 cells in which the cGMP-dependent pathway was activated by hemin. (elsevierpure.com)
  • These results suggested that the cAMP-dependent pathway, which is independent of MAPK pathways, plays a negative role in γ-globin gene expression in K562 cells. (elsevierpure.com)
  • Furthermore, by primer extension of single-stranded oligonucleotide probes, we show that the theta 1-globin gene of humans is transcribed in an erythroleukemia cell line K562. (ox.ac.uk)
  • Haemin treatment of the K562 cells slightly enhances the level of the longest theta 1-transcript. (ox.ac.uk)
  • Using human K562 erythroleukemia cells capable of differentiating along erythroid and megakaryocytic lineages, we explore the differentiation-related role of heat shock transcription factor 2 (HSF2), which belongs to a family of transcription factors generally known to regulate heat shock gene expression. (abo.fi)
  • Our results reveal a multistep regulatory process of HSF2 gene expression, In K562 cells undergoing hemin-mediated erythroid differentiation, the increase in HSF2 protein levels is preceded by transcriptional induction of the HSF2 gene, accompanied by increased HSF2 mRNA stability. (abo.fi)
  • cAMP and cGMP may have differential roles in the regulation of γ-globin gene expression in erythroid cells. (elsevierpure.com)
  • Our results provide strong evidence that the theta 1-globin gene of humans is transcriptionally active in cells of erythroid origin, and suggests the presence of a functional theta 1-polypeptide in specific cells, possibly those of early erythroid tissue. (ox.ac.uk)
  • The up-regulation of miR-199b-5p during erythroid differentiation was dependent on the binding of GATA-1 and NF-E2 to its gene locus, which activated its transcription and maintained its high expression level in mature erythroid cells. (molcells.org)
  • These genes were identified using a comparative genome analysis of 215 strains belonging to ten human-related Haemophilus (sub)species (training dataset). (biomedcentral.com)
  • Interestingly, s479 is not only encoded next to CRISPR-cas genes but the mature s479 contains a crRNA-like 5´ handle and experiments with Cas protein deletion strains indicate maturation by Cas6 and interaction with Cas proteins. (uni-stuttgart.de)
  • The Tar II (= ATCC 30267) LV9 and LV39 strains had been expanded in SDM-79 moderate (12) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Wisent St. Bruno Quebec Canada) and 5 μg/ml of hemin. (bioxorio.com)
  • Both GATA-1 and NF-E2 bound upstream of the miR-199b gene locus and activated its transcription. (molcells.org)
  • The sRNA gene is encoded between a CRISPR RNA locus and the Cas protein gene cluster, the s479 deletion strain is viable and was characterized in detail. (uni-stuttgart.de)
  • Expression of γ-globin mRNA was examined by primer extension, and transcriptional activity of the γ-globin gene promoter was determined by reporter gene assays. (elsevierpure.com)
  • Activation of the cAMP-dependent pathway by forskolin inhibited hemin-induced expression of γ-globin mRNA and decreased transcriptional activity of the γ-globin gene promoter. (elsevierpure.com)
  • To counterbalance oxidative stress and ensure redox homeostasis, a subgroup of non-small-cell lung cancers acquire stabilizing mutations in NFE2L2 , the gene encoding NRF2, the transcriptional regulator of the cellular antioxidant response, and inactivating mutations in its negative regulator, KEAP1. (nature.com)
  • The transcriptional regulator BACH1 negatively controls the expression of antioxidant genes, counteracting NRF2 activity. (nature.com)
  • Searching for a transcriptional driver that might be responsible for the gene enrichment led to the identification of NRF2 and BACH1. (nature.com)
  • 19. Mara C D,Huang T,Irish V F. The Arabidopsis floral homeotic proteins APETALA3 and PISTILLATA negatively regulate the BANQUO genes implicated in light signaling[J].Plant Cell,2010,22(3):690-702. (nefu.edu.cn)
  • Significant changes in gene expression of proteins that mediate oxidative metabolism, such as cytochrome d ubiquinol oxidase-encoding genes, cydA and cydB , were detected. (microbiologyresearch.org)
  • Three of the six up-regulated genes encode potential zinc transporter proteins (ZnuA1, ZnuB1, ZnuC1) suggesting involvement of s479 in regulation of zinc transport. (uni-stuttgart.de)
  • The primary endpoint of the study was reduction relative to placebo in the annualized rate of composite porphyria attacks, defined as those requiring hospitalization, urgent healthcare visit, or intravenous hemin administration at home, in patients with acute intermittent porphyria, the most common subtype of AHP, over six months. (globalgenes.org)
  • Hemin (plasma-derived intravenous heme) is the definitive treatment and mainstay of management. (medscape.com)
  • Consistent with a reduction in ROS levels, NAC-K cells also exhibited downregulation of genes associated with antioxidant processes, in particular the target genes of NRF2 and BACH1. (nature.com)
  • these authors noted that BACH1 protein levels were higher in NAC-K cells than con-K cells and that this could be reversed by the addition of haemin, a haem derivative. (nature.com)
  • When cancer cells are treated with hemin, BACH1 is reduced, causing BACH1-depleted cancer cells to change metabolic pathways," said co-author Jiyoung Lee, PhD, an instructor affiliated with the Rosner laboratory. (uchicagomedicine.org)
  • Members of the genus Haemophilus are small, pleomorphic gammaproteobacteria that are characterized by their dependency on the blood factors haemin (X-factor) and/or nicotinamide adenine dinucleotide (V-factor) for growth [ 1 , 2 ]. (biomedcentral.com)
  • The primary ingredients of the phenol-inactivated fermentation medium for Haemophilus influenzae include an extract of yeast, nicotinamide adenine dinucleotide, hemin chloride, soy peptone, dextrose, and mineral salts and for Neisseria meningitidis include an extract of yeast, amino acids and mineral salts. (theodora.com)
  • H. influenzae , a fastidious, pleomorphic, gram-negative coccobacillus, requires hemin (X factor) and nicotinamide-adenine-dinucleotide (NAD, also known as V factor) for in vitro growth. (cdc.gov)
  • We sought to test the effects of simultaneous expression of a combination of three selected human genes, namely Heme Oxygenase 1 (hHO1), Ecto 5' Nucleotidase (hCD73) and Ecto Nucleoside Triphosphate Diphosphohydrolase 1 (hCD39), involved in the down-regulation of the inflammatory response, in an in vitro model of TNF--induced injury. (unimib.it)
  • The protective effect given by the simultaneous expression of hHO1, hCD73 and hCD39 genes against TNF-alpha toxicity is characterized by the up-regulation of specific genes inhibiting the NfkB pathway. (unimib.it)
  • Transcriptome studies of wild type Haloferax cells and the deletion mutant revealed up-regulation of six genes in the deletion strain, showing that the sRNA has a clearly defined function. (uni-stuttgart.de)
  • Heparanase also augments cell adhesion and signaling cascades leading to enhanced phosphorylation of selected protein kinases and increased transcription of genes associated with aggressive tumor progression. (pafr-signal.com)
  • We found that this novel combination, hemin plus metformin, can suppress tumor growth, and we validated this in mouse tumor models. (uchicagomedicine.org)
  • The role of DNA methylation in the mediation of multiple tumor suppressor gene and microRNA silencing has been implicated in the development and progression of MM ( 7 ). (spandidos-publications.com)
  • The existence of G-quadruplex DNA is also heavily implied in the promoter region of genes and oncogenes, and is thus assumed to play an important regulatory role in their transcription [ 26 ]. (hindawi.com)
  • Differentiation of multipotential hematopoietic cells into lineage-committed precursors involves the selection and maintenance of appropriate programs of gene expression, regulated by specific transcription factors. (abo.fi)
  • This can suppress transcription of mitochondrial electron transport chain genes, a key source of cellular energy. (uchicagomedicine.org)
  • Methylation is a fundamental regulator of gene transcription. (spandidos-publications.com)
  • DNA methylation, a form of epigenetic control of gene transcription, refers to cytosine methylation at position 5 in the pyrimidine ring, which can result in inappropriate silencing of genes involved in diverse biological processes, including cell proliferation, apoptosis, migration and cell cycle arrest ( 5 ). (spandidos-publications.com)
  • The researchers applied hemin, an inducer of heme oxygenase-1 (HO-1), to increase nitric oxide production. (isaaa.org)
  • Porphyria of Doss is caused by a deficiency of delta-aminolevulinic acid dehydratase (ALA-D, the second enzyme in the heme biosynthesis pathway) that leads to an accumulation of the precursors of porphyrins in the liver (delta-aminolevulinic acid, ALA). The enzyme deficiency is caused by mutations in the ALAD gene coded for ALA-D (to date, 9 mutations are known and expressed). (orpha.net)
  • 2. Carpenter R,Coen E S. Floral homeotic mutations produced by transposon-mutagenesis in Antirrhinum majus [J].Genes Dev,1990,4(9):1483-1493. (nefu.edu.cn)
  • Triple negative breast cancer (TNBC) also disproportionately affects younger women, women of African descent and women with mutations in the BRCA1 gene. (uchicagomedicine.org)
  • The concomitant reduction of expression of a gene encoding the lactate transporter PG1340 suggests decreased utilization of this nutrient. (microbiologyresearch.org)
  • Also, genes encoding gingipain protease secretion/maturation regulator ( porR ) and protease transporter ( porT ) had reduced expression in the presence of oxygen, which also correlated with reduced protease activities under aerobic conditions. (microbiologyresearch.org)
  • RNA sequencing of cell lines generated from KP and KPK lung tumours revealed upregulation of metastatic genes in Keap1 mutant cells. (nature.com)
  • Upregulation of these genes in the deletion strain was confirmed by northern blot and proteome analyses. (uni-stuttgart.de)
  • Cytochrome c oxidase subunit 4 isoform 2, mitochondrial is an enzyme that in humans is encoded by the COX4I2 gene. (wikipedia.org)
  • It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. (wikipedia.org)
  • Fetal hemoglobin inducers such as hemin, butyrate, and hydroxyurea stimulate γ-globin gene expression by activating the cyclic GMP (cGMP)-dependent pathway. (elsevierpure.com)
  • Although cGMP activates the cyclic AMP (cAMP)-dependent pathway by suppressing cGMP-inhibited phosphodiesterase 3 (PDE3), the effects of the cAMP-dependent pathway on γ-globin gene expression are unknown. (elsevierpure.com)
  • The differences in the activation of TNF-a signaling pathway were analysed at molecular level by using an RT2 Profiler TM PCR array system in which the expression of 84 TNF-related pathway genes were evaluated by real-time PCR. (unimib.it)
  • Gene ontology and pathway analysis revealed reduced secondary metabolic capabilities and loss of function in terms of signalling and response to environmental cues, consistent with adaptation for the extraintestinal niche. (biomedcentral.com)
  • By PCR, the AtPI gene was integrated into the tobacco genome. (nefu.edu.cn)
  • Therefore, we established an accurate and rapid whole genome sequencing (WGS) based classification and serotyping algorithm and combined it with the detection of resistance genes. (biomedcentral.com)
  • In the genome of NCCP15653, genes encoding major virulence factors of uropathogenic E. coli were detected. (elsevierpure.com)
  • The black pigmentation characteristic of this bacterium comes from iron acquisition that does not use the typical siderophore system of other bacteria but accumulates hemin.Peptides appear to be the predominant carbon and energy source of this organism, perhaps in keeping with its ability to destroy host tissue. (up.ac.za)
  • This resulted to significant expression of several structures and regulatory genes involved in anthocyanin production. (isaaa.org)
  • Furthermore, hemin treatment raised nitric oxide contents in radish sprouts, probably through enhancing nitrate reductase activity and Nitric Oxide-Associated 1 (NOA1) expression. (isaaa.org)
  • A gene presence/absence-based classification algorithm was developed, which employs the open-source gene-detection tool SRST2 and a new classification database comprising 36 genes, including capsule loci for serotyping. (biomedcentral.com)
  • ATRX can bind the variable number of tandem repeats (VNTR) sequence in the promoter region of the α-globin gene cluster. (jascoinc.com)
  • Besides differing by 26% in replacement-site substitutions, the theta 1 and alpha 1-globin genes of the orangutan and baboon also differ in their promoter structures, in the use of TGA versus TAA as the termination codon, and in the use of AGTAAA versus AATAAA as the polyadenylation signal. (ox.ac.uk)
  • Apart of stimulatory elements along the heparanase promoter, we identified AU-rich element in the 3' untranslated region that suppresses heparanase gene expression. (pafr-signal.com)
  • The promoter region of MEG3 is rich in CpG islands, and the specific methylated and unmethylated CpG islands of differentially methylated regions (DMRs) are located upstream of the MEG3 gene (IG-DMR and MEG3-DMR) ( 17 , 18 ). (spandidos-publications.com)
  • Gene reduction analysis revealed that the gene deletion processes are under selective pressure, and many of the inactivations are probably related to the organism's interaction with its host environment. (asm.org)
  • Barbaro M, Kotajärvi M, Harper P, Floderus Y. Identification of an AluY-mediated deletion of exon 5 in the CPOX gene by MLPA analysis in patients with hereditary coproporphyria. (medscape.com)
  • Both theta 1-globin genes are split into three exons with the potential to code for a polypeptide of length 141 amino acids. (ox.ac.uk)
  • The virulence of this bacterium results from the 32 Y pestis chromosomal genes and two Y pestis -specific plasmids, constituting the only new genetic material acquired since its evolution from its predecessor. (medscape.com)
  • Here we present the complete DNA sequence of a cloned theta 1-globin gene of humans, and show that it contains no apparent defects that would abolish its expression. (ox.ac.uk)
  • The NAC-K cells had an increased ability to migrate and invade compared with con-K cells, which was concomitant with an enrichment of genes involved in cell motility and migration. (nature.com)
  • ΔH as -43.65 kJ mol -1 and ΔS as -50.65 Jmol -1 K -1 at 25°C. The presence of four hemopexin-binding domains in the gene sequence indicated possible binding to hemin. (omicsonline.org)
  • On the other hand, genes encoding AslA, OmpA, and the K1 capsule, which are virulence factors associated with invasion of neonatal meningitis-causing E. coli, were also present, while a gene encoding CNF-1 protein, which is a cytotoxic necrotizing factor 1, was not detected. (elsevierpure.com)
  • Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
  • Resistance genes were detected in 16 out of 262 datasets from the German cohort. (biomedcentral.com)
  • The activity of the hCD39/hCD73 genes in TNF--treated TG cells maintained up-regulated the anti-inflammatory Tnfaip3 gene and the activity of hHO1 in TNF--treated TG cells up-regulated the anti-apoptotic Traf3 gene as compared to WT cells. (unimib.it)
  • In addition, the presence of antibiotic resistance genes within the German dataset was evaluated with SRST2 and correlated with results of traditional phenotyping assays. (biomedcentral.com)
  • A definitive diagnosis is dependent on the identification of a causal mutation of the ALAD gene. (orpha.net)
  • METHODS AND RESULTS: A triple cistronic vector, named pCX TRI-2A, harbouring hHO-1, hCD73 and hCD39 coding sequences, has been produced by cloning in frame the sequences of the three genes by using the F2A-based technology. (unimib.it)
  • In contrast, the 16S rRNA gene sequences from 158T and 78-1T were only 95 and 90? (woofahs.com)
  • When an acute attack is confirmed, urgent treatment with an injection of human hemin and/or perfusion of carbohydrates is required. (orpha.net)
  • In the pivotal study, Givlaari was shown to significantly reduce the rate of porphyria attacks that required hospitalizations, urgent healthcare visits or IV hemin administration at home compared to placebo. (globalgenes.org)
  • Nanjing Agricultural University scientists investigated the impact of hemin treatment on anthocyanin production in radish sprouts. (isaaa.org)
  • Diagnostic strategies for autosomal dominant acute porphyrias: retrospective analysis of 467 unrelated patients referred for mutational analysis of the HMBS, CPOX, or PPOX gene. (medscape.com)
  • The gene content of NCCP15653, a group D strain, demonstrates that it could be both uropathogenic E. coli and neonatal meningitis-causing E. coli. (elsevierpure.com)
  • A portion of the hepatitis B virus gene, coding for HBsAg, is cloned into yeast, and the vaccine for hepatitis B is produced from cultures of this recombinant yeast strain according to methods developed in the Merck Research Laboratories. (theodora.com)
  • The antigen is harvested and purified from fermentation cultures of a recombinant strain of the yeast Saccharomyces cerevisiae containing the gene for the adw subtype of HBsAg. (theodora.com)
  • Structure and expression of the human theta 1 globin gene. (ox.ac.uk)
  • In contrast, the two theta 1-globin genes from primates only differ by 1.7% in the replacement-site substitutions. (ox.ac.uk)
  • In order to explore the function of PI gene in the development of petals and stamens, the PI gene in Arabidopsis thaliana was used as the research object, and the AtPI gene was amplified from cDNA by PCR, and the plant expression vector(pROKⅡ- AtPI ) was constructed and transformed into tobacco. (nefu.edu.cn)
  • Construction of Plant Expression Vector and Genetic Transformation Analysis of Arabidopsis thaliana AtPI Gene in Nicotiana tabacum [J]. Bulletin of Botanical Research, 2016, 36(3): 388-394. (nefu.edu.cn)
  • As an effective vehicle for bio-research and for gene therapy, Lentiviral Vector (LV) has been drawn large attention in recent years. (whocc.org.cn)
  • We inserted hPARP1 gene into the pFastBac1, a baculovirus transfer vector and then transformed it into DH10Bac containing a shuttle vector of Bacmid. (whocc.org.cn)
  • Analysis of the APETALA3- and PISTILLATA -like genes in Hedyosmum orientale (Chloranthaceae) provides insight into the evolution of the floral homeotic B-function in angiosperms[J].Ann Bot,2013,112(7):1239-5121. (nefu.edu.cn)
  • Microarray analysis revealed alteration in gene expression in the presence of 6 % oxygen. (microbiologyresearch.org)
  • The COX4I2 gene produces a 20 kDa protein composed of 171 amino acids. (wikipedia.org)
  • a process which can be reversed by 5-Aza-2'-deoxycytidine (5-Aza-CdR), suggesting a potential therapeutic agent for MM. The maternally expressed 3 (MEG3) imprinted gene is located on chromosome 14q32, which produces a non-coding RNA transcript ( 11 ). (spandidos-publications.com)
  • This effect is more evident and further increased in TG cells over-expressing the three genes. (unimib.it)
  • The simultaneous expression of the three genes has been demonstrated at RNA level by RT-PCR and at protein level by immunofluorescence, fluorescence activated cell sorting (FACS) and western blotting. (unimib.it)
  • During the exponential growth phase, 96 genes were upregulated and 79 genes were downregulated 1.4-fold. (microbiologyresearch.org)