Genes in cells. Medical search. Frequent questions

In this work, 119 overlapping differentially expressed genes (DEGs) were obtained from GSE5281 and GSE122063 datasets through differential expression analysis. (hindawi.com)
The identity of differentially expressed genes suggested that more resilient, stably surviving DA neurons were enriched in samples from advanced stages of disease, either as a consequence of positive selection of a less vulnerable long-term surviving mDA neuron subtype or due to up-regulation of neuroprotective gene products. (frontiersin.org)
For this purpose, the GSE2503 was downloaded from the Gene Expression Omnibus database, and the differentially expressed genes (DEGs) between 6 normal skin and 5 SCC samples were analyzed using the Linear Models for Microarray Data package. (spandidos-publications.com)
Padilla et al ( 3 ) and Nindl et al ( 7 ) selected differentially expressed genes (DEGs) between SCC and normal skin (NO) samples using the microarray expression profile dataset GSE2503. (spandidos-publications.com)

These cycling stem cells regularly generate progeny, which subsequently exit the niche and pass through the "common origin of differentiation" around position +5, where they commit toward the various individual lineages. (wikipedia.org)
Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine. (wikipedia.org)
The groundbreaking study grew out of Wang's desire to better understand the epigenetic regulation of stem cell differentiation, in which the structure of genes is modified while the sequence of the DNA is not. (news-medical.net)
He and his team found that KDM4B and KDM6B, two gene-activating enzymes, can promote stem cells' differentiation into bone cells by removing methyl markers from histone proteins. (news-medical.net)
The findings imply that chemical manipulation of these gene-activating enzymes may allow stem cells to differentiate specifically into bone cells, while inhibiting their differentiation into fat cells. (news-medical.net)
The research group, through its study of aging mice, found that the two enzymes KDM4B and KDM6B could specifically activate genes that promote stem cell differentiation toward bone, while blocking the route toward fat. (news-medical.net)
The researchers used genome-wide genetic screening to both overexpress and inactivate ("knock out") tens of thousands of genes that govern embryonic stem cell proliferation and differentiation into the three germ layers. (genengnews.com)
The expression of these genes is correlated most closely with lymphoid cell differentiation and growth stimulation in a variety of cell types. (nih.gov)
Similarly, rel family proteins control a number of genes involved in lymphoid cell growth and differentiation. (nih.gov)
Thus, the Freiburg researcher hopes to gain a better understanding of the extent to which noise regulates development or is even necessary for the differentiation of cells. (mpg.de)
Essential genes - such as transcription factors that mediate which genes should be switched on or off - are often only weakly expressed in cell differentiation, sometimes with high variability in cells of the same type. (mpg.de)
But the biological implications of gene expression noise during cell differentiation and cell state transitions have not been explored in depths," says Grün. (mpg.de)
With this approach, it is possible to explore the dynamics of gene expression noise during the differentiation of stem cells into mature cell types and to investigate the extent to which noise regulates development or is even necessary for cellular differentiation. (mpg.de)
We assume that the fluctuating activity of these gene networks - in other words, the noise of these genes - could trigger the differentiation," says Dominic Grün. (mpg.de)
reported that hematopoietic stem and progenitor cells gave rise to iPSCs up to 300 times more efficiently than terminally differentiated B and T cells did, suggesting that the differentiation state of the starting cells affects reprogramming efficiency 23 . (nature.com)
The epigenetic mechanisms that control chromatin organization are known to regulate cell differentiation in both animals and plants. (europa.eu)
Colony of stem cells genetically modified with a double fail-safe mechanism to control the formation of tumors and the differentiation into non-desirable cell types. (diabetesresearch.org)
Research has shown that only about 30 - 40 percent of beta-like cells are obtained through current PSC differentiation (development) protocols, leaving a significant percentage of undefined cells in the balance. (diabetesresearch.org)
It arises from the uncontrolled proliferation of epithelial cells or cells exhibiting cytological or tissue architectural characteristics of SC differentiation, including the presence of keratin, tonofilament bundles or desmosomes, which are structures involved in cell-cell adhesion ( 1 ). (spandidos-publications.com)

Diagram shows steps involved in conducting gene therapy for sickle cell disease. (nih.gov)
Researchers at the National Institutes of Health have developed a new and improved viral vector-a virus-based vehicle that delivers therapeutic genes-for use in gene therapy for sickle cell disease. (nih.gov)
The development of the vector could make gene therapy for sickle cell disease much more effective and pave the way for wider use of it as a curative approach for the painful, life-threatening blood disorder. (nih.gov)
Our new vector is an important breakthrough in the field of gene therapy for sickle cell disease," said study senior author John Tisdale, M.D., chief of the Cellular and Molecular Therapeutic Branch at the National Heart, Lung, and Blood Institute (NHLBI). (nih.gov)
Researchers have used virus-based vehicles for years in gene therapy experiments, where they have been very effective at delivering therapeutic genes to bone marrow stem cells in the lab before returning them to the body. (nih.gov)
With gene therapy, doctors modify the patient's bone marrow hematopoietic (blood-producing) stem cells in the lab by adding a normal copy of the beta-globin gene through the use of a viral vector. (nih.gov)
Gene therapy trials using reverse-oriented vectors for sickle cell disease and beta-thalassemia have largely been encouraging, the researchers said, but this complicated gene translation process has made vector preparation and gene-transfer efficiency more difficult. (nih.gov)
Q: Two companies, Bluebird Bio and Vertex Pharmaceuticals, just received FDA approval of their gene therapy protocols. (nih.gov)
FDA approval of the gene therapy protocols will reduce acute care utilization and improve the overall health and wellbeing of those living with sickle cell disease. (nih.gov)
Approval by the FDA of the first gene therapy for individuals living with SCD will provide a very effective disease-modifying treatment that has been shown to be life-changing for individuals living with SCD. (nih.gov)
Q: Can you briefly and in plain language describe how these two gene therapy approaches will work? (nih.gov)
The two gene therapy trials recently approved for SCD use different ways to change the expression of an individual's genes but both therapies result in an individual making more fetal hemoglobin, a type of oxygen-carrying blood protein present at birth. (nih.gov)
For example, leukemia is a very rare complication of gene therapy in adults with SCD. (nih.gov)
NHLBI investigators are working to identify the molecular basis of, and advance possible solutions for, blood cancers associated with gene therapy. (nih.gov)
This Request for Applications (RFA), Gene Therapy for Sickle Cell Disease, is related to the priority areas of clinical prevention services, chronic disabling conditions, and maternal and infant health. (nih.gov)
An important goal of this program is to attract new and established investigators into the field of gene therapy for sickle cell disease by providing access to critical technologies in the form of shared facilities and funds to pursue innovative pilot/feasibility studies. (nih.gov)
Pilot/feasibility studies, when combined with appropriate core support, will provide established investigators who have not previously worked in gene therapy and new young investigators with state-of-the-art core technologies that will enable them to be competitive in the field. (nih.gov)
Our goal in this series, as well as the e-book on which they are based, has been to provide insights on current logistics best practices for global cell and gene therapy studies. (worldcourier.com)
For an in-depth look at logistics for personalized medicine, including cell and gene therapy trials, please download our e-book, Tomorrow's Medicine: Curing One Patient at a Time . (worldcourier.com)
THIS March, gene therapy made a significant breakthrough-scientists at the Oregon Health & Science University in Portland, United States, used the gene-editing tool Crispr-Cas9 to edit DNA inside a human body for the first time, also called in vivo gene editing. (deloitte.com)
Our analysis shows that, in the decade leading up to 2020, six major pharma companies alone made more than 50 cell and gene therapy alliances and licensing deals worth over $16 billion and spent approximately $38 billion on acquisitions. (bcg.com)
Cell and gene therapy companies can leverage new practices such as remote monitoring for long-term follow-up and develop master protocols to accelerate trials. (bcg.com)
In addition, the mRNA vaccines developed by Pfizer and Moderna have led to improved cold-chain manufacturing, distribution, and storage capabilities throughout the sector, providing a much stronger foundation for cell and gene therapy products that have similar requirements. (bcg.com)
In 2020, six cell and gene therapy programs were forced to delay their development timelines after the FDA asked for more information about their production processes. (bcg.com)
Requests for additional data delayed one cell therapy company's program by four to six months. (bcg.com)
Accelerating and streamlining Cell & Gene Therapy enrollment and coordination across the entire patient journey. (accenture.com)
Our unique industry solution connects stakeholders in the Cell & Gene Therapy value chain, enabling manufacturers, supply chain partners and Healthcare Providers (HCPs), to work in a harmonized, standardized way. (accenture.com)
Multi-tenant by design, the purposeful architecture of INTIENT Unify Cell & Gene orchestrates patient enrollment, reduces cost and the potential risks of therapy coordination-improving time-to-therapy. (accenture.com)
One of the most exciting areas of innovation is Cell and Gene Therapy, with many therapies already changing the industry and patients' lives. (accenture.com)
SINGAPORE, Sept. 19, 2023 (GLOBE NEWSWIRE) -- Novotech , the leading Asia Pacific centred biotech CRO with global execution capabilities has just been awarded for the second consecutive year the Best CRO for Cell & Gene Therapy 2023 at the Asia Pacific Cell & Gene Therapy Excellence Awards . (woodtv.com)
Novotech's Vice President, Clinical Services Dr. Yooni Kim accepted the award at the 7 th Cell & Gene Therapy World Asia conference last week in Singapore. (woodtv.com)
Novotech is also the recipient of the Best CRO for Cell & Gene Therapy 2022 award. (woodtv.com)
Accepting the award, Dr. Kim thanked the organizers IMAPAC and stated "Novotech is proud to be recognised as an exceptional cell & gene therapy (CGT) CRO leader that facilitates rapid advancement in these new therapies. (woodtv.com)
She remarked "according to our latest CGT clinical trial landscape report the global CGT market is projected to hit $93.78 billion by 2030 and FDA approvals for gene therapy in H2 2022 surpassed the combined total for the previous five years. (woodtv.com)
As the gene and cell therapy market continues to develop, targeted solutions to address cost of care and support clinical outcomes are beginning to emerge. (mercer.com)
With this foundation, North Carolina's strengths in biological manufacturing naturally created a cluster of gene and cell therapy manufacturing, including Novartis Gene Therapies, Audentes, an Astellas company, and more. (wral.com)
Gene therapy alleviates the underlying cause of genetic diseases and acquired diseases. (wral.com)
Each cell and gene therapy is designed based on detailed information about the roots of a patient's disease. (wral.com)
Rather than treating symptoms, gene therapy works by repairing or enhancing cells at the genetic level. (wral.com)
According to Weirich, the aim of gene therapy is to halt a disease in its tracks or reverse its progress, as opposed to simply alleviating symptoms. (wral.com)
Already, its initial gene therapy for spinal muscular atrophy has been approved in the U.S., Japan, the European Union, Israel, Brazil and Canada. (wral.com)
That treatment, the company's flagship gene therapy in neuroscience, Zolgensma, was approved in May 2019 in the U.S. to treat children less than two years old with SMA. (wral.com)
The healthcare industry in general - and gene therapy in particular - is incredibly fast-paced and changing every day. (wral.com)
The company is currently implementing its plans to construct a $109 million, state-of-the-art gene therapy manufacturing facility that employs 200. (wral.com)
It serves as the basic instructions that allow you to create antibodies, or enzymatic proteins, or RNA molecules with your gene therapy. (wral.com)
What gene therapy does is it takes a benign virus and gets rid of the viral elements that allow the virus to replicate and make multiple copies of itself. (wral.com)
Distinct gene expression signatures emerged in leukemia cells that eventually died from intensive treatment, and ceased growing due to maintenance therapy. (cedars-sinai.edu)
The Digital Capability Center New Jersey uses a mock autologous cell therapy manufacturing setup with authentic production equipment, where operators walk through the manufacturing process in a realistic setting. (mckinsey.com)
This could be an autologous chimeric antigen receptor (CAR) T cell-based gene therapy, or any one of the many allogeneic products in clinical development. (onenucleus.com)
In the case of cell and gene therapy products, the manufacturing step is the development plan - at least initially. (onenucleus.com)
The manufacturing step differs depending on whether the prospective therapy is autologous, or based on cells or tissues from a patient, or allogeneic where cells are taken from different individuals of the same species and prepared for the patient. (onenucleus.com)
The autologous approach is illustrated by Kymriah, the pioneering CAR T cell therapy for acute lymphoblastic leukaemia which was approved by the US Food and Drug Administration in 2017. (onenucleus.com)
For example, BlueRock Therapeutics LP is using induced pluripotent stem cells (iPSCs) as source material for a stem cell therapy for Parkinson's disease. (onenucleus.com)
Right now there's a lot of excitement about gene therapy, and currently there's a LentiGlobin BB305 product that is used to treat severe sickle cell disease using the gene therapy mechanism. (consultantlive.com)
Dr Kanter, can you talk about the Mark Walters, MD, abstract and what we should look forward to with the gene therapy for sickle cell disease? (consultantlive.com)
It's important to know that there's 2 different types, really more than 2, but 2 kind of buckets of gene therapy. (consultantlive.com)
One is what we would consider gene addition therapy. (consultantlive.com)
One of the big concerns with gene addition therapy is that that gene could insert and turn on a cancer gene, and we're not seeing that, which is really encouraging. (consultantlive.com)
That's really exciting because I think that's the biggest challenge we've had with gene therapy for other conditions, right? (consultantlive.com)
The other big excitement about gene therapy is that it doesn't have the problems that we spoke about earlier in terms of stem cell transplant, and that all patients don't need a donor, they're their own donor. (consultantlive.com)
It's quite interesting in that it's a single hairpin RNA knockdown approach, so it's really the first time that's been used in gene therapy, which is quite exciting. (consultantlive.com)
SINGAPORE , Sept. 18, 2023 /PRNewswire/ -- On September 14 , 2023, Biosyngen Pte Ltd (hereinafter referred to as "Biosyngen") was the recipient of the prestigious "Most Promising Cell Therapy Pipeline in APAC" award at the 7th Annual Cell & Gene Therapy World Asia 2023, amidst a pool of international biotechnology companies. (wowktv.com)
We are very proud to be acknowledged as a prominent global leader in the field of cell and gene therapy, particularly in the Asia Pacific region, for our dedication to research and innovation in immunotherapy. (wowktv.com)
Professor Jean Paul Thiery expressed his gratitude for the committee's acknowledgement, emphasizing the potential of cell and gene therapy in driving groundbreaking advancements and boundless opportunities within the industry. (wowktv.com)
He also highlighted the importance of enhancing communication and collaboration among all stakeholders to address industry challenges and facilitate the swift progress of gene and cell therapy medications. (wowktv.com)
Looking to the future, he anticipates more successful clinical trials and the approval of more innovative cell and gene therapy which ultimately benefit a larger population of patients. (wowktv.com)
Cell and gene therapy has emerged as a prominent area of focus within the global pharmaceutical industry. (wowktv.com)
Notably, the FDA has granted approval to a greater number of gene therapy drugs in the latter half of 2022 alone compared to the prior five-year period. (wowktv.com)
Market analysis indicates that the cell and gene therapy market is growing at a compound annual growth rate (CAGR) of 22% and is expected to be worth $100 billion by 2030. (wowktv.com)
As a global Biotech headquartered in Singapore and China , Biosyngen is steadfast in its dedication to the development of innovative and impactful cell and gene therapy products, with the aim of benefiting patients worldwide. (wowktv.com)
PALO ALTO, Calif. & RESEARCH TRIANGLE PARK, N.C.--( BUSINESS WIRE )-- Kriya Therapeutics, Inc. , a biopharmaceutical company developing gene therapies for conditions affecting millions of people around the world, today announced that it will present 10 abstracts at the 26th Annual Meeting of the American Society of Gene and Cell Therapy ( ASGCT ) taking place in Los Angeles, CA, May 16-20, 2023. (businesswire.com)
In type 1 diabetes, the insulin-producing islets cells of the pancreas have been mistakenly destroyed by the immune system, requiring patients to manage their blood sugar levels through a daily regimen of insulin therapy. (diabetesresearch.org)
Brussels South Charleroi BioPark (BSCB) recently announced it has launched a Cell and Gene Therapy Accelerator program to de-risk early stage research and create the advanced therapy companies of the future. (onenucleus.com)
Enhancing good manufacturing practice (GMP), customer compliance and efficient aseptic manufacturing is essential as new advanced therapy medicinal products (ATMPs), including cell, gene and biological products advance through clinical trials to commercialisation. (pharmiweb.com)
NEW ORLEANS - A gene therapy recently made headlines for becoming the most expensive pharmaceutical ever launched - the price tag was $3.5 million for a one-off treatment with etranacogene dezaparvovec (Hemgenix) for hemophilia B . (medscape.com)
A gene therapy for sickle cell anemia is in late clinical development and could be on the market soon. (medscape.com)
However, it has the potential to be a cost-effective therapy for patients with sickle cell disease (SCD) in the United States, say investigators who analyzed the costs using a novel method that takes into account historical heath inequities. (medscape.com)
Our study shows that, when we compare the costs of gene therapy and existing standard-of-care treatment for SCD using a technique that accounts for historical health disparities, gene therapy could be an equitable therapeutic strategy for all patients with SCD, whether their disease is mild, moderate, or severe," he said. (medscape.com)
When you look over time at the cost for a patient to get gene therapy vs the standard of care, it might actually be beneficial to have the gene therapy," she said. (medscape.com)
She noted, however, that some patients start gene therapy for SCD at older ages and that it's important to analyze whether the treatment can still be cost-effective or the best therapeutic option for such patients. (medscape.com)
The model assumes that a single course of gene therapy for SCD would cost $2.1 million. (medscape.com)
The estimate was based on the cost of US Food and Drug Administration-approved gene therapies, and it was assumed that the therapy would result in permanent disease remission for all patients. (medscape.com)
In addition, the model assumed that all eligible patients in the US with SCD who are aged 12 years and older would be offered the gene therapy. (medscape.com)
In their base-case analysis, gene therapy starting at age 12 would yield 25.5 discounted lifetime quality-adjusted life years (QALYs) at a cost of $2.4 million, compared with 16.0 discounted lifetime QALYs at a cost of $1.1 million for standard care. (medscape.com)

Embryonic stem cells could help treat all kinds of disorders, and editing the genomes of these stem cells could make the treatments far more potent. (newscientist.com)
A team at Novartis has found that genome editing kills most human embryonic stem cells - and that the ones that do survive are likely to have mutations in a key anti-cancer gene. (newscientist.com)
Gene networks in human embryonic stem cells (hESCs) have been found to serve two purposes at once. (genengnews.com)
This discovery, from a study led by researchers from Brigham and Women's Hospital and Harvard Medical School (HMS), suggests that faulty embryonic stem cells have a built-in mechanism to ensure that they are destroyed before they can compromise the functioning of future cells and tissues. (genengnews.com)
Instead of looking at genes one by one, we looked at thousands of genetic alterations at the same time to determine how they affect the proliferation of embryonic stem cells, and, subsequently, the development of the three germ layers that serve as the raw material for human tissues. (genengnews.com)

In advanced lab tests using animal models, the new vector was up to 10 times more efficient at incorporating corrective genes into bone marrow stem cells than the conventional vectors currently used, and it had a carrying capacity of up to six times higher, the researchers report. (nih.gov)
The scientists have discovered two key epigenetic regulating genes that govern the cell-fate determination of human bone marrow stem cells. (news-medical.net)
Through our recent discoveries on the lineage decisions of human bone marrow stem cells, we may be more effective in utilizing these stem cells for regenerative medicine for bone diseases such as osteoporosis, as well as for bone reconstruction,' Wang said. (news-medical.net)

In the developing brain, cell adhesion proteins enable neurons to migrate to the correct places and connect with other neurons. (nih.gov)
This causes the stem cell to go dormant and stop producing new neurons. (sciencedaily.com)
This response is actually very good for the stem cells because the outside environment is not ideal for newly born neurons," Dr. Paik explained. (sciencedaily.com)
It's better for stem cells to remain dormant and wait until the stress is gone to produce neurons. (sciencedaily.com)
Overall, the researchers found, gene expression of glial cells changed more with age than did that of neurons. (the-scientist.com)
Typically we have concentrated on neurons for studies of dementia, as they are the cells involved in brain processing and memories. (the-scientist.com)
Based on previous data about the genes expressed by different cell types, the team sorted the expressed genes into those specific to neurons and those specific to glia. (the-scientist.com)
Gene expression changed to a greater extent with age in glial cells than in neurons, the researchers found. (the-scientist.com)
The researchers concluded that the gene-expression changes in neurons and oligodendrocytes are attributable both to changes in cell number and to expression levels in individual cells. (the-scientist.com)
The expression of glial cell-specific genes was a better predictor of age than that of neurons, the researchers found, underscoring the relevance of non-neuronal cells to aging. (the-scientist.com)
Isolated mDA neurons were subjected to genome-wide gene expression analysis by mRNA sequencing. (frontiersin.org)
Our main interest is beta cells, but it could also be tailored to select for neurons, heart or liver cells, for example," explained Dr. Dominguez-Bendala. (diabetesresearch.org)

MAL2 promotes proliferation, migration, and invasion through regulating epithelial-mesenchymal transition in breast cancer cell lines. (nih.gov)
Inhibition of lung epithelial cell proliferation by hyperoxia. (jci.org)
Posttranscriptional regulation of proliferation-related genes. (jci.org)
The regulation of T2 cell proliferation and the effect of reactive oxygen (O2) species on this lung cell proliferation have not been well defined. (jci.org)
Hyperoxia for 24 to 48 h arrested cell proliferation in a SV40T-immortalized T2 cell line we have developed and in primary and SV40T-immortalized lung fibroblasts. (jci.org)
Thus proliferation arrest induced by hyperoxia was associated with posttranscriptional control of at least two late cell cycle-related genes. (jci.org)
This form of proliferation arrest is also seen when primary and SV40T-T2 cells but not fibroblasts are serum deprived, suggesting that T2 cells in vitro may be uniquely sensitive to alterations in their redox state and that these alterations in turn affect translational control of a subset of proliferation-related genes. (jci.org)
However, downregulation of METTL3 restrained the proliferation, migration and invasion of NSCLC cells. (nature.com)
Fig. 5: Suppression of SFRP2 abolished the potency of METTL3 knockdown in NSCLC cell proliferation, migration and invasion. (nature.com)
We investigated biological behaviors such as cell cycle, proliferation, radiation sensitivity, apoptosis and migration, hypoxia signal pathway in the lung cancer cells treated with chronic intermittent hypoxia, as well as the role of hypoxia inducible factor 1 there, hypoxia-inducible genes analyzed by real-time RT-PCR chip in H446 cells treated with the model. (nih.gov)

For the past 30 years, researchers have been designing these beta-globin vectors in a reverse structural orientation, meaning the therapeutic genes incorporated into the virus are translated, or "read," from right to left by the viral vector-making machinery - much like reading an English sentence backwards. (nih.gov)
Decades of basic research on sickle cell disease by scientists-including NIH-supported researchers- and selfless efforts by clinical trial participants have helped lay the groundwork for these novel genetic approaches. (nih.gov)
Researchers, patients, clinicians, and advocacy groups expect these new FDA-approved approaches to help people with sickle cell disease live longer, less painful, and more productive lives. (nih.gov)
Researchers have identified two types of cells in our brains that are involved in organizing discrete memories based on when they occurred. (nih.gov)
Using an experimental drug, researchers were able to suppress a mutated amyotrophic lateral sclerosis (ALS) gene. (nih.gov)
Columbia University Medical Center (CUMC) researchers have created a way to develop personalized gene therapies for patients with retinitis pigmentosa (RP), a leading cause of vision loss. (nih.gov)
We collaborated with researchers from Egypt, Syrian Arab Republic and Jordan in a study of patients with sickle-cell disease from those countries, and from various parts of Saudi Arabia, in order to investigate the influence of genetics on the clinical presentation of the disease, and to attempt to determine the origin of the sickle-cell gene in Arabs. (who.int)
Finally, the researchers observed that many of the genes that regulate the formation of the three germ layers also are known contributors to the growth of cancers when they are over- or underexpressed in somatic cells. (genengnews.com)
The researchers found that a group of about 30 genes that encode cell adhesion proteins (including cadherins) were more strongly associated with ASD than all other genes in their data set. (nih.gov)
The researchers found that many of these rare genetic glitches, known as copy number variations, affect genes involved in cell adhesion. (nih.gov)
The researchers suggest this system may influence ASD by altering the turnover of adhesion proteins at the cell surface. (nih.gov)
Researchers will report on the discovery of genetic defects that cause particular types of non-Hodgkin lymphoma (NHL), making these genes rational targets for new anti-lymphoma treatments, in the next issue of of the journal Blood. (lymphomainfo.net)
Researchers have found that defects in one gene (TCL1) can cause particular types of non-Hodgkin lymphoma. (lymphomainfo.net)
The researchers then engineered mice to aberrantly express the TCL1 gene and those mice developed B-NHLs at a very high rate, within the germinal centers of lymphoid tissues, such as lymph nodes and the spleen, where B cells normally develop. (lymphomainfo.net)
The researchers believe that the number and variety of switches are optimized to help the cancer cells survive and grow, Dr. Scacheri explained. (cancer.gov)
Using a mouse model, the researchers explored how cells respond when both genes are inhibited. (genengnews.com)
The first enabled the researchers to read the gene expression signatures of individual cancer cells from patients with leukemia. (cedars-sinai.edu)
By demonstrating that cancer cells with distinct gene expression signatures tend to grow in different organs and bone marrow pockets, the study also underscored a major problem facing cancer researchers: studying non-representative samples of patient cells. (cedars-sinai.edu)
During this 'minimal cell project', the researchers switch off one gene after another and study the effects on the microbes. (lindau-nobel.org)
Researchers describe how gene expression in different human brain regions is altered with age. (the-scientist.com)
To learn what happens within the aging brain and potentially gain information relevant to human health, researchers examined gene-expression patterns in postmortem brain samples. (the-scientist.com)
The researchers examined gene-expression data from 10 brain regions of 480 brain samples from people who died at ages ranging from 16 to 106 and who had not been diagnosed with neurodegenerative disease. (the-scientist.com)
To determine whether age-related changes in neuronal and glial cell numbers affected gene expression results, the researchers used imaging to count various cell types in tissue samples from the frontal cortices of young and older brains. (the-scientist.com)
MIAMI, FL - February 19, 2019 - Researchers have been advancing methods to generate insulin-producing beta cells from pluripotent stem cells (PSC) for the clinical treatment of type 1 diabetes (T1D). (diabetesresearch.org)

This initiative encourages research to develop new, or adapt existing, computational and statistical approaches to aggregate, mine, and conduct integrative analyses of single-cell and bulk tissue multi-omic datasets from brain samples (e.g., gene expression, chromatin state, histone modifications, DNA methylation, etc.) to achieve new insights into the biology of psychiatric disorders. (nih.gov)
To investigate this process we focused on the regulation of two late cell cycle genes, histone and thymidine kinase, in T2 cells and fibroblasts exposed in vitro to varying periods of hyperoxia (95% O2). (jci.org)
Post-translational histone modifications contribute to the maintenance of chromatin states associated with a defined gene expression program. (europa.eu)
Among histone modifiers, the evolutionary conserved Polycomb Group (PcG) machinery has a key role in repressing the genes that are not required in a specific cell fate, thus, establishing cell identity. (europa.eu)

Sickle cell disease is an inherited blood disorder caused by a mutation, or misspelling, in the beta-globin gene (or β-globin gene). (nih.gov)
This segment is required for high-level beta-globin gene expression but gets clipped out during the normal vector preparation process if it is left in the natural, forward direction. (nih.gov)
In contrast to the old vector, the gene sequence, or "message," of the new beta-globin vector is read from left to right - like reading a normal sentence - making the gene translation approach less complicated, Tisdale explained. (nih.gov)
They found that the new vectors could transfer a much higher viral load - up to six times more therapeutic beta-globin genes than the conventional vectors - and had four to 10 times higher transduction efficiency, a measure of the ability to incorporate corrective genes into repopulating bone marrow cells. (nih.gov)
We also have a gene addition approach using a lentiviral vector, but instead of inserting the modified LentiGlobin beta-globin type, we're actually trying to use a different non-sickling globin. (consultantlive.com)

The National Human Genome Research Institute provides a definition of gene regulation in their Talking Glossary of Genetic Terms. (medlineplus.gov)
The Genetic Science Learning Center at the University of Utah offers an explanation of gene expression as it relates to disease risk . (medlineplus.gov)
The genetic manipulation of the individual components is obtained by whole-body and conditional gene ablation in developing embryos and adult mice. (nih.gov)
The sickle cell patients are subjected to several factors causing inflammation, and the genetic identification of each individual allows to focus the possibility of allelic variations influence of a specific gene and then the polymorphism. (nih.gov)
Integrating genetic variation with cell-type specific variation in transcript and protein abundance to make causal inferences about potential molecular targets. (nih.gov)
In recent years, gene-based treatments have been groundbreaking for rare genetic disorders and infectious diseases," said Dr. Trevor Mundel, president of global health at the Gates Foundation. (nih.gov)
Globally, cell and gene therapies (CGT) are transforming not just how humans treat genetic and intractable diseases but are altering the entire pharmaceutical ecosystem. (deloitte.com)
When the cell cycle proceeds without control, cells can divide without order and accumulate genetic errors that can lead to a cancerous tumor . (medlineplus.gov)
The team reported in Nature on April 28, 2009, that several genetic variants were associated with ASD, all pointing to a spot between 2 genes, called CDH9 and CDH10, on chromosome 5. (nih.gov)
He and his colleagues are now planning a more extensive genome-wide association study to gain a more complete picture of the genes and genetic interactions involved in ASD. (nih.gov)
While the exact cause of AD has not been elucidated, studies have found that AD is the result of a combination of genes, lifestyle, and environmental factors, caused in part by specific genetic changes [ 6 - 9 ]. (hindawi.com)
In the new NCI-funded study, scientists found that brain cancer cells also slip several different genetic "on switches"-pieces of DNA that help activate genes-into these DNA circles. (cancer.gov)
At Novartis Gene Therapies, the company's goal is to develop and commercialize gene therapies for patients and families affected by rare and life-threatening neurological genetic diseases. (wral.com)
Lentiviral vector is the predominant delivery vehicle used for introducing genetic modifications into cell therapies such as CAR-T and HSC gene therapies. (lonza.com)
Twenty-three gene therapies have been FDA-approved with promising results for treating diseases including blood cancers, hemophilia, congenital blindness, and spinal muscular atrophy, which is the leading genetic cause of death in infants. (mckinsey.com)
As soon as a cell receives a new genetic program, it starts to put this program to use. (lindau-nobel.org)
Thanks to the introduced genetic modification, the first two paths bring about the destruction of any cell that takes them. (diabetesresearch.org)

Signals from the environment or from other cells activate proteins called transcription factors. (medlineplus.gov)
This finding is consistent with our current understanding that cancer usually involves defects in more than one critical gene, including defects that activate cancer-causing genes (oncogenes) like TCL1 and defects that inactivate cancer-preventing genes (tumor suppressors). (lymphomainfo.net)
Depending on type and stage of development, cells activate different genes, which are initially translated into RNA molecules that form the basis for the synthesis of proteins. (mpg.de)
These switches also activate EGFR in cells that lack amplification of the gene, they found. (cancer.gov)
Unlike the other two switches, these elements are typically isolated from EGFR on the chromosome and don't normally activate the gene. (cancer.gov)

Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed on the 119 DEGs, revealing some important biological functions and key pathways. (hindawi.com)
Gene Ontology (GO) and pathway enrichment analysis of DEGs were performed, followed by functional annotation and construction of a protein‑protein interaction (PPI) network. (spandidos-publications.com)

Specifically, we use a range of molecular, cellular and animal models to look at the effects of mutations on proteins and of variants or missing proteins on cells and on the function of the aging nervous system. (nih.gov)
When they put these cells into mice, those that were provoked to have higher levels of inflammation logged more mutations in their DNA. (newscientist.com)
By injecting the CRISPR protein into zebrafish embryos, they could use the random mutations they created to trace which cells go on to form which organs. (newscientist.com)
Thus, it is not surprising that mutations in rel genes have been implicated directly and circumstantially in a number of lymphoid malignancies, as have a number of other cellular and viral transcription factors (see also Cleary M, Hayman M and Beug H, this issue and reviewed in Rabbitts, 1991). (nih.gov)
The mutations that allow cancer cells to divide uncontrollably also make the cancer cells more dependent on certain cellular processes," notes senior author, Robert Weiss, associate professor of molecular genetics. (genengnews.com)

Gene regulation can occur at any point during gene expression, but most commonly occurs at the level of transcription (when the information in a gene's DNA is passed to mRNA). (medlineplus.gov)
This involves measuring the number of mRNA molecules generated by active genes in the individual cells. (mpg.de)
Just like a kind of fingerprint, the number of different mRNA molecules per gene in a particular cell informs about the cell's identity and the relationship between the cells. (mpg.de)
RT-qPCR was applied to determine mRNA of m6A-associated genes and SFRP2, and western blot were used for ZEB1 and MMP9 protein expression. (nature.com)

This now well-defined model thus represents a new biological system in which to test the effect of novel genes on initiation and progression of cancer, as well as new anticancer compounds, without the limitations intrinsic to monolayer cultures of tumor cells (eg, events involving adhesion molecules and 3-dimensional organization of a tumor). (medscape.com)
For example, in an aggressive type of brain cancer called glioblastoma , tumor cells typically have many copies of the EGFR oncogene . (cancer.gov)
A vast diversity of individual tumor cells exists in every patient with cancer. (cedars-sinai.edu)
These leukemia models do not capture the full diversity of individual tumor cells within a single patient, let alone within the broader population of patients affected by this disease. (cedars-sinai.edu)
To investigate the tumor cells' adaptation to hypoxia, we recreated two models using two lung cancer cell lines in the presence of intermittent hypoxia, which is characterized by changes in oxygen pressure within the disorganized vascular network. (nih.gov)

The process of turning genes on and off is known as gene regulation. (medlineplus.gov)
Gene regulation is an important part of normal development. (medlineplus.gov)
Gene regulation also allows cells to react quickly to changes in their environments. (medlineplus.gov)
Although we know that the regulation of genes is critical for life, this complex process is not yet fully understood. (medlineplus.gov)
The Khan Academy has an educational unit on gene regulation , including videos about gene regulation in bacteria and eukaryotes. (medlineplus.gov)
The goal of this conference is to bring together scientists from many different disciplines interested in the regulation of gene expression and cell signaling by nutrient and non-nutrient dietary factors. (nih.gov)
The initial sessions of the meeting are organized around the central themes of molecular regulation of gene expression by nutrients, such as glucose and fatty acids, by vitamins and minerals, and by phytochemicals and xenobiotics. (nih.gov)
The goal of this initiative is to encourage research that will advance our understanding of brain cell type-specific gene regulation to elucidate the pathogenesis of mental disorders. (nih.gov)
Tight regulation of this process ensures that a dividing cell's DNA is copied properly, any errors in the DNA are repaired, and each daughter cell receives a full set of chromosomes. (medlineplus.gov)
Disruption of normal regulation of the cell cycle can lead to diseases such as cancer. (medlineplus.gov)
Roundtree IA, Evans ME, Pan T, He C. Dynamic RNA modifications in gene expression regulation. (nature.com)

The initiative's goal is to advance safe, effective and durable gene-based cures to clinical trials in the U.S. and SSA within the next seven to 10 years. (nih.gov)
But even as the agents make impressive clinical progress-the alliance counts 1,220 clinical trials underway last year-cell and gene therapies continue to face big scientific, technological , and business-related hurdles. (bcg.com)
With clinical trials already initiated using PSC-derived beta cell progenitors and newer approaches with functional beta-like cells on the horizon, the need to ensure patient safety is of paramount importance. (diabetesresearch.org)

Despite this remarkable progress, it is clear that a significant number of genes that regulate circadian rhythms in mammals remain to be discovered and identified. (nih.gov)

When genes known to control pluripotency were deleted-genes such as OCT4 and SOX2-the hESCs surprisingly increased their resistance to death, indicating that under normal circumstances pluripotency regulators also contribute to apoptosis pathways. (genengnews.com)
Analyses of gene expression in cells affected by neurodegenerative disease can provide important insights into disease mechanisms and relevant stress response pathways. (frontiersin.org)
The present study aimed to investigate the genes and signaling pathways associated with squamous cell carcinoma (SCC) by bioinformatics analysis. (spandidos-publications.com)
DEGs of SCC were primarily enriched in pathways associated with cancer and cell adhesion. (spandidos-publications.com)
A number of genes and signaling pathways involved in the progression of SCC have been previously identified ( 3 , 6 - 9 ). (spandidos-publications.com)

Tracing cell lineages is crucial for understanding how our genomes develop bodies from a single cell. (newscientist.com)
There are other methods for tracing cell lineages, but these all have limitations, and can involve spending many years painstakingly watching embryos under a microscope . (newscientist.com)
This process occurs through the activation of certain genes favoring a commitment to one lineage and the concurrent deactivation of genes favoring other lineages. (news-medical.net)

The NIH Brain Research Through Advancing Innovative Neurotechnologies® (BRAIN) Initiative Cell Census Network (BICCN) has unveiled an atlas of cell types and an anatomical neuronal wiring diagram for the mammalian primary motor cortex, derived from detailed studies of mice, monkeys, and humans. (nih.gov)
More recently modern genetics techniques, primarily using transgenic mice, have been used to identify genes that are specifically expressed or highly enriched in the intestinal stem cells. (wikipedia.org)
In 2018, Dr. Paik and her team showed that mice who lack the FOXO3 gene in their brain are unable to cope with stressful conditions in the brain, which leads to the progressive death of brain cells. (sciencedaily.com)
By using the VarID method, the author was able to track the activity of essential transcription factors during the development of blood cells in mice. (mpg.de)
Using this experimental system, the team analyzed the gene expression signatures of a representative sample of barcoded leukemia cells, and then transplanted the remainder of the cells into mice. (cedars-sinai.edu)
Distinct gene expression signatures correlated with the various organs where the cancer cells ended up in the mice. (cedars-sinai.edu)
There are similar challenges when patient cells are transplanted into laboratory mice in order to conduct pre-clinical cancer research. (cedars-sinai.edu)
Less than 1% of patient cells grow and multiply in mice. (cedars-sinai.edu)
Intriguingly, the anti-tumor functions of METTL3 knockdown in the phenotype of NSCLC cells and xenograft mice were overturned by inhibition of SFRP2. (nature.com)

In this study, we used high-throughput sequencing data for differential gene expression analysis, GO functional and KEGG pathway enrichment analyses, and protein-protein interaction (PPI) network analysis to identify network hub genes and their biological roles. (hindawi.com)

These proteins bind to regulatory regions of a gene and increase or decrease the level of transcription. (medlineplus.gov)
These include new alleles of known circadian genes as well as novel circadian loci such, Fbxl3, an orphan member of the F-box protein family, which we have found to interact selectively with the CRY proteins to target them for degradation through the proteasome pathway. (nih.gov)
Both genes encode cadherins - cell-surface proteins that help cells adhere to each other. (nih.gov)
In addition, rel proteins are likely to be involved in T cell diseases caused by the human retroviruses HTLV-I and HIV-1. (nih.gov)

For example, therapeutic immune cells could be engineered to patrol a person's body, recording what they see and reporting back to clinicians when they are recaptured. (newscientist.com)
The group's research could pave the way toward identifying potential therapeutic targets for stem cell-mediated regenerative medicine, as well as the treatment of bone disorders like osteoporosis, the most common type of metabolic bone disease. (news-medical.net)
Therefore, it is key to identify the hub genes, explore the pathogenesis, and search for the therapeutic targets of AD. (hindawi.com)

Apoptosis of cells in the luminal spaces was associated with selective activation of caspase 3 in cells critically located near the lumen to be. (medscape.com)
If a cell has an error in its DNA that cannot be repaired, it may undergo self-destruction ( apoptosis ). (medlineplus.gov)
Apoptosis is a common process throughout life that helps the body get rid of cells that no longer work or that it doesn't need. (medlineplus.gov)
Apoptosis protects the body by removing genetically damaged cells that could lead to cancer, and it plays an important role in the development of the embryo and the maintenance of adult tissues. (medlineplus.gov)
Paraquat-induced apoptosis in human neuroblastoma SH-SY5Y cells: involvement of p53 and mitochondria. (nih.gov)
They maintain pluripotency, and they keep apoptosis, or programmed cell death, on a hair trigger. (genengnews.com)
The cells mediated by irradiation induced resistance to radiation and apoptosis and increased metastasis in lung cancer cells. (nih.gov)

The ability to control whether certain stem cells ultimately become bone cells holds great promise for regenerative medicine and potential therapies aimed at treating metabolic bone diseases. (news-medical.net)
As pluripotent cells-progenitors of every type of cell type in the body-hESCs are of central interest to developmental and regenerative biologists. (genengnews.com)
The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) by defined transcription factors has been a well-established technique and will provide an invaluable resource for regenerative medicine. (nature.com)

In the adult intestine, the crypts of Lieberkühn are the niche for epithelial stem cells and contain all proliferative stem and progenitor cells. (wikipedia.org)
Later work suggested that these "+4 cells" may function as reserve or back-up stem cells, and further suggested that they divide slowly relative to the other progenitor cells in the crypt. (wikipedia.org)
However, these stem/progenitor cells are generally inaccessible, require complicated procedures to obtain and are difficult to propagate in vitro . (nature.com)

Growth of malignant cells can be evaluated in 2 different types of experimental models: conventional tissue cultures and animal models with human breast cancer xenografts. (medscape.com)
We have used tissue-specific, conditional gene expression methods to analyze the relative contributions of central and peripheral circadian oscillators to circadian organization. (nih.gov)
In silico methods could be utilized to leverage the strengths of high-depth multi-omic information generated from bulk tissue brain samples in conjunction with the more precise genomic information provided by single-cell approaches. (nih.gov)
It is thus a prime opportunity to integrate new single-cell datasets with existing bulk tissue brain data resources such as GTEx, ENCODE, and the PsychENCODE Consortium (PEC). (nih.gov)
The measurements can be used to analyze the spatial arrangement of individual cells in the tissue and to identify developmental trajectories and transitional states on these trajectories. (mpg.de)
Our research spans several focus areas related to cell, gene and tissue engineering, including stem cell engineeing research. (buffalo.edu)
The University at Buffalo offers a number of graduate degree programs and research opportunities related to career paths in cell, gene and tissue engineering areas. (buffalo.edu)
According to the authors, the present study, which used array-based gene-expression data and tissue samples from the UK Brain Expression Consortium and the North American Gene Expression Consortium, represents the largest analysis of aging and gene expression in the human brain. (the-scientist.com)

The second allowed the scientists to label individual leukemia cells with heritable, DNA-based 'barcodes,' offering a way to track not only the cells but also their progeny during disease progression. (cedars-sinai.edu)
We were able to identify previously unknown genes that are involved in disease progression and chemotherapy resistance,' said Merchant, director of the Imaging Mass Cytometry Core at Cedars-Sinai Cancer and an associate professor of Medicine . (cedars-sinai.edu)
Exosome-transmitted lncRNA UFC1 promotes non-small-cell lung cancer progression by EZH2-mediated epigenetic silencing of PTEN expression. (nature.com)
Thus, an improved understanding of the underlying molecular mechanisms and gene networks involved in the development and progression of skin SCC is required. (spandidos-publications.com)
During tumor progression, multiple genes experience up- or downregulation ( 7 ). (spandidos-publications.com)

Using circadian gene reporter methods, one can demonstrate that most peripheral organs and tissues can express circadian oscillations in isolation, yet still receive and may require input from the dominant circadian pacemaker in the suprachiasmatic nucleus (SCN) in vivo. (nih.gov)
When they inactivated the Hus1 gene in healthy mammary gland tissues, it caused genome damage and cell death. (genengnews.com)
Pluripotent stem cells are known to develop into all of the body's cells and tissues. (diabetesresearch.org)

The rel family of genes encodes transcription factors, such as the v-rel oncoprotein and cellular transcription complexes (eg NF-kappa B), consisting of c-rel and related polypeptides. (nih.gov)
This gene encodes a multispan transmembrane protein belonging to the MAL proteolipid family. (nih.gov)

Moreover, currently available analysis methods are almost exclusively focused on quantifying and interpreting gene expression levels within an individual cell. (mpg.de)

AD gene expression data were obtained from Gene Expression Omnibus (GEO) database [ 19 ] ( https://www.ncbi.nlm.nih.gov/gds ). (hindawi.com)

Scientists call this phenomenon "biological noise" and suspect that it is also decisive for the fate of cells, i.e. the developmental pathway a cell takes. (mpg.de)

Cells expressing CSF-1 receptors also showed an invasive phenotype with hyperproliferation of breast cells, formation of cellular masses, and disruption of intercellular junctions when exposed to CSF-1. (medscape.com)
Advances in analysis of single-cell technologies, while still in development, are leading investigators to map the cellular diversity of the brain at single-cell resolution (e.g. (nih.gov)
Other variations involved genes in the ubiquitin-proteasome system, a cellular waste disposal system. (nih.gov)

Furthermore, common variation in many genes contributes to risk of sporadic PD. (nih.gov)
In most cases, it's likely that each gene contributes a small amount of risk, and interacts with other genes and environmental factors to trigger the onset of disease," Dr. Hakonarson says. (nih.gov)
That discovery led Dr. Jihye Paik, associate professor of pathology and laboratory medicine at Weill Cornell Medicine, and her colleagues to investigate how this gene contributes to brain health during aging. (sciencedaily.com)

Identification of genes associated with tumor suppression in Syrian hamster embryo cells. (nih.gov)
In recent years, scRNA-seq not only confirmed already known cell types but also led to the identification of formerly unknown and rare cell types. (mpg.de)

We develop general operating principles and conduct gene network modeling based on genome-wide experimental data. (nih.gov)

Apply through a new funding opportunity to use somatic cell gene editing (SCGE) approaches to improve graft survival and outcomes for recipients of allogenic solid organ, pancreatic islet, or vascularized composite allograft (VCA) transplants. (nih.gov)
NIAID and the National Heart, Lung, and Blood Institute (NHLBI) cosponsor the Notice of Special Interest (NOSI): Somatic Cell Gene Editing Therapies To Improve Transplantation Outcomes initiative. (nih.gov)
Furthermore, how plants regenerate a complete organism from a single somatic cell remains a mystery. (europa.eu)

Many scientists thought that simply having several copies of an oncogene could cause a cell to become cancerous, explained Ian Fingerman, Ph.D., of the DNA and Chromosome Aberrations Branch in NCI's Division of Cancer Biology , who wasn't involved in the study. (cancer.gov)
It's possible that, in DNA circles, contacts between genes and enhancers that normally would have been prevented in the chromosome are driving cancer cell growth via additional boosts to gene expression," Dr. Fingerman noted. (cancer.gov)

Cite this: Conference Report - Plenary Session: From Genes to Cells to Metastasis - Medscape - Aug 14, 2003. (medscape.com)
Those cells display distinct behaviors related to cancer growth, metastasis and responses to chemotherapy. (cedars-sinai.edu)

In particular, this approach reveals the activity of weak and noisy transcription factors involved in cell state transitions. (mpg.de)
The data shows that important transcription factors known to be expressed in mature blood cells in the mouse bone marrow are lowly expressed - but highly variable - in blood stem cells. (mpg.de)
First, we attempted to generate iPSCs from DP31 cells, a cell line isolated from the immature (CC) stage of teeth, using as few transcription factors as possible. (nature.com)

To develop a new system that would mimic more accurately events related to cell growth in vivo and offer at the same time the opportunity of high through-put analysis, Dr. Brugge and colleagues [ 3 ] further developed the epithelial breast acini model, initially proposed by Mina Bissell, using MCF-10A breast cancer cells. (medscape.com)
In accordance with the stem cell zone model proposing that, during their upward migration, CBC stem cells would only gradually lose their self-renewal capacity, it was shown in vivo that transient amplifying cells can revert to Lgr5+ CBC stem cells after damage, presumably by direct contact with Paneth cells. (wikipedia.org)
It aims to treat diseases by replacing, inactivating or introducing genes into cells- either inside the body, or in vivo, or outside of the body, or ex vivo,' said Jimmy Weirich, vice president and site head at Novartis Gene Therapies, a global healthcare company with a major manufacturing facility in Durham. (wral.com)

Scientists report they have created a tiny, nanosize container that can slip inside cells and deliver protein-based medicines and gene therapies of any size -- even hefty ones attached to the gene-editing tool called CRISPR. (nih.gov)
We'll soon know - the CRISPR gene editing technique has been adapted to make cells keep a log of what happens to them, written inside their own DNA. (newscientist.com)
Such CRISPR-based logging could have a huge range of uses, from smart cells that monitor our health from within, to helping us understand exactly how our bodies develop and grow. (newscientist.com)
They designed a system where CRISPR can be activated within a cell whenever it experiences a particular event - for example, exposure to a particular chemical. (newscientist.com)
CRISPR-based logging could help us understand what happens inside cells that turn cancerous, for instance, or help us monitor the levels of substances in the body. (newscientist.com)
And North Carolina State University's Rodolphe Barrangou, Ph.D., uses a different CRISPR enzyme to edit genes. (wral.com)

Thus, VarID delineates neighborhoods with differential gene expression variability, also in complex mixtures of different cell types or cell states. (mpg.de)

Generally, neuron- and oligodendrocyte-specific genes showed decreased expression with age, while the expression of microglia-specific genes increased with age. (the-scientist.com)

Fig. 2: Knockdown of METTL3 alleviated the malignant traits of NSCLC cells. (nature.com)

Diet plays a critical role in human health and disease and understanding the underlying molecular mechanisms by which dietary components influence cell biology and physiology is essential. (nih.gov)
3 Department of Developmental and Cell Biology, University of California, Irvine, CA 92617, United States of America. (nih.gov)

Cell and gene therapies have proved their potential only in rare diseases with well-defined genomic targets, high unmet need, and small numbers of patients. (bcg.com)
The authors' effort in this comprehensive work is a 'genomic tour de force,' showing that, overall, non-neuronal cells undergo gene expression changes at a larger scale than previously thought in aging," Andras Lakatos , a neuroscientist at the University of Cambridge, U.K., who was not involved in the study, wrote in an email. (the-scientist.com)

These gene-expression changes were most significant in the hippocampus and substantia nigra, regions damaged in Alzheimer's and Parkinson's diseases, respectively, according to the study published today (January 10) in Cell Reports . (the-scientist.com)

Previous studies have shown that cancer cells could behave in the form of adaptation to hypoxia in tumor growth, which could result in their biological changes and determine their responses to the therapies. (nih.gov)
Addressing the shortage of insulin-producing cells would address one of the major challenges that stand in the way of a biological cure for the disease. (diabetesresearch.org)

For example, AlloVir, a Boston-based company developing cell therapies targeting life-threatening viral diseases, recently announced it is developing allogeneic T-cell therapies for COVID-19 in collaboration with the Baylor College of Medicine. (deloitte.com)

To carry out these behaviors, each cancer cell uses its genes to construct the needed molecules in a unique way known as its 'gene expression signature. (cedars-sinai.edu)

In mammals, circadian oscillations are generated by a set of genes forming a transcriptional autoregulatory feedback loop: these include: Clock, Bmal1, Per1, Per2, Cry1, Cry2 and Casein kinase 1 epsilon. (nih.gov)

These genes may provide new targets for future therapies. (cedars-sinai.edu)

The approach, the first of its kind, takes advantage of induced pluripotent stem (iPS) cell technology to transform skin cells into retinal cells, which are then used as a patient-specific model for disease study and preclinical testing. (nih.gov)
Now, for the first time, scientists from the Diabetes Research Institute (DRI) at the University of Miami Miller School of Medicine have engineered a human pluripotent stem cell line containing two 'suicide genes' that induce cell death in all but the desired insulin-producing cells. (diabetesresearch.org)
To address these concerns, the DRI team set out to engineer pluripotent stem cell lines that selectively destroy both tumorigenic cells and cells that do not produce insulin, like liver, brain, muscle cells and others. (diabetesresearch.org)

There are few disease-modifying and life-extending therapies for those living with sickle cell disease, leaving patients burdened by chronic pain, high financial costs, health complications, and higher risk of early mortality. (nih.gov)
HCPs can log in to Unify to onboard their Cell & Gene patients to sponsor therapies for which they are qualified. (accenture.com)
Using cancer cell samples from lymphoma patients, Dr. Teitell and his colleagues discovered that abnormal TCL1 expression is a frequent abnormality in B-NHL. (lymphomainfo.net)
By comparison, the allogeneic approach relies on a single source of cells to treat many patients and is often described as an 'off-the-shelf' approach. (onenucleus.com)
All the way through from A to C, we know that it's persistent, so some of our patients who were in that first group are now 3, 3-and-a-half years out and they have that same amount of gene, of that new gene being expressed. (consultantlive.com)
However, transplantation of these immature cells into patients poses substantial risks, namely the development of tumors (teratomas) and the growth of these stem cells into unwanted cell types. (diabetesresearch.org)
Islet transplantation has restored natural insulin production in people with type 1 diabetes, as DRI scientists have published, but there are not enough cells to treat the millions of patients who can benefit. (diabetesresearch.org)
Goshua and colleagues included equity weight in an analysis of 10 years of data on annual healthcare costs for patients with SCD who were covered by private insurance and were treated with medications (for example, hydroxyurea), antibiotics, blood transfusion, and hematopoietic stem cell transplants. (medscape.com)

Moreover, the technology also allows further insights into the cell. (mpg.de)

This modification sends the protein into the nucleus of the stem cell where it turns on stress response genes. (sciencedaily.com)
This nutrient is needed to help a protein called lamin form a protective envelope around the DNA in the nucleus of the stem cell. (sciencedaily.com)

After injury, repair of the alveolar epithelium is dependent on the ability of epithelial type 2 (T2) cells to proliferate. (jci.org)

Firstly, the gene expression matrices of GSE5281 and GSE122063 datasets were normalized and formatted into input file format of R language. (hindawi.com)

Differentially expressed genes4

Differentiation19

Therapy84

Embryonic Stem5

Bone marrow stem cells3

Neurons12

Proliferation10

Researchers24

Histone4

Beta-globin5

Genetic17

Activate5

Ontology2

Mutations5

MRNA4

Tumor cells5

Regulation11

Clinical trials3

Regulate1

Pathways5

Lineages3

Mice9

Analyses1

Proteins4

Therapeutic3

Apoptosis7

Regenerative3

Progenitor Cells3

Tissue8

Progression5

Tissues3

Encodes2

Interpreting gene expression1

Expression Omnibus1

Pathway1

Cellular3

Contributes3

Identification2

Genome-wide1

Somatic cell3

Chromosome2

Metastasis2

Transcription factors3

Vivo3

CRISPR6

Differential gene expression1

Neuron1

Malignant1

Biology2

Genomic2

Parkinson's1

Biological2

Viral1

Molecules1

Transcriptional1

Targets1

Pluripotent3

Patients8

Insights1

Nucleus2

Epithelial1

Datasets1