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  • hybridization
  • Nucleic acid hybridization has been used in an attempt to determine the fraction of spleen DNA which is homologous to immunoglobulin messenger RNA (mRNA). (jimmunol.org)
  • We have analyzed more than 180 VH gene-containing recombinant clones from an unamplified spleen cDNA library by selective sequencing of JH and CH positive clones following iterative hybridization screening with family-specific VH probes, 11 unique families of VH genes, each associated with a unique genomic Southern blot hybridization pattern, are described and compared. (rupress.org)
  • Hybridization with a λ-like gene-specific DNA probe to somatic cell hybrids revealed that these genes reside on 22q11.2 between the breakpoint cluster region ( BCR ) and the Ewing sarcoma breakpoint at 22q12 and that gene 16.1 was located distal to genes 14.1 and 16.2 . (springer.com)
  • diversification
  • in Aim 2, we will use sterile GALT explants and TLR and NLR agonists and antagonists to determine the contribution of TLR and NLR ligands and IgM-binding bacterial superantigen-like molecules to B cell proliferation and Ig gene diversification;we will also use sterile explants of GALT as well as an in vitro FDC:B cell culture system to determine how complement activation contributes to GALT development. (grantome.com)
  • In Aim 3, we will search in gnotobiotic CD4-/- mice for GALT-like B cells that proliferate in an antigen and T-cell independent manner and undergo somatic diversification of the Ig genes. (grantome.com)
  • The phenotypic similarities include a shift from HR-mediated diversification to single-nucleotide substitutions in the immunoglobulin variable gene segment and the partial reversion of this shift by overexpression of Rad51. (asm.org)
  • bone marrow
  • The regulated expression of B-lineage associated genes during B-cell differentiation in bone marrow and fetal liver. (springer.com)
  • neoplastic
  • Immunoglobin Genes is the first comprehensive book on the structure, function, and expression of the genes encoding antibodies in normal and neoplastic cells. (worldcat.org)
  • polypeptide
  • When bacterial lipopolysaccharide was added to the growth medium, levels of mRNA and polypeptide increased, showing regulated expression of the introduced ĸ gene. (caltech.edu)
  • antibodies
  • The immunoglobin gene complex is responsible for generating an extraordinary wide range of antibodies, each possessing a unique antigen specificity. (worldcat.org)
  • lymphocyte
  • During differentiation of a given B lympho- cyte stem cell, an immunoglobulin gene is assembled containing one L-V, one J, and one C segment, and this gene is transcribed by the lymphocyte and all of its progeny. (damasgate.com)
  • proteins
  • The genes that encode these proteins are assumed to have evolved from a common ances- tral gene over a period of hundreds of millions of years. (damasgate.com)
  • segments
  • The arrows indicate the direction of transcription of the gene segments. (asmscience.org)
  • withmultiple V gene segments preceeding several D segments, a number of J segments followed by theCµ region. (diva-portal.org)
  • These genes are remarkable in that they are made up of segments that are shuffled as the B lymphocytes ma- ture. (damasgate.com)
  • There are about 300 L-V segments per light chain gene, and each of the V segments has a different base se- quence. (damasgate.com)
  • chain
  • There are four IG gene types, variable (V), diversity (D) (only for IGH), joining (J) and constant (C) genes which contribute to the IG chain synthesis. (genenames.org)
  • The variable domain at the N-terminal end of each IG chain results from a V-(D)-J rearrangement whereas the remaining of the chain, or constant region, is encoded by a C gene. (genenames.org)
  • Plasmid pSV2gpt-κ41, containing the ĸ light chain gene from the myeloma MOPC41 and the selectable marker gene gpt, was introduced into 81A-2 cells by the calcium phosphate coprecipitation technique. (caltech.edu)
  • Bauer, Jr., T. R. and Blomberg, B. A new λ-like light chain gene, 16.2, is related to genes 14.1 and 16.1 expressed in pre-B cells. (springer.com)
  • Blomberg, B. B., Rudin, C. M., and Storb, U. Identification and localization of an enhancer for the human λL chain Ig gene complex. (springer.com)
  • infection
  • There are many instances in human biology where positive and negative selection forces can act to shape the immunoglobulin repertoire and therefore repertoire analysis can provide useful information on infection control, vaccination efficacy, autoimmune diseases and cancer. (surrey.ac.uk)
  • Cell
  • Recently, considerable progress has been achieved in assembling gene regulatory networks comprised of signaling components and transcription factors that regulate B-cell development. (springer.com)
  • Included in the su- perfamily are the immunoglobulins (q.v.) with up to 12 domains per molecule, the T cell receptors (q.v.), and the MHC receptors (q.v.), each with two do- mains per molecule, and the CD4 and CD8 recep- tors (q.v.) with four domains and a single domain, respectively. (damasgate.com)
  • It is widely accepted that HCV drives the expansion of few B-cell clones expressing a restricted pool of selected immunoglobulin variable (IgV) gene subfamilies frequently endowed with rheumatoid factor (RF) activity. (hindawi.com)