• Luciferase reporter assay was performed to verify that miR-455 directly binds to the 3′-noncoding region (UTR) of RAB18 mRNA in Huh7 cells. (hindawi.com)
  • miR-455 inhibited cell viability and invasion by directly targeting the 3′-UTR of RAB18 mRNA of hepatocellular carcinoma. (hindawi.com)
  • MicroRNAs (miRNAs) are small noncoding endogenous RNAs containing 19 to 25 nucleotides that promote posttranscriptional control in regulating the expression of target gene by binding to the 3′-UTR sequences of its mRNA [ 5 ]. (hindawi.com)
  • The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. (nih.gov)
  • This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. (nih.gov)
  • The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (nih.gov)
  • miRNAs are highly conserved, small, non-coding RNAs that negatively regulate gene expression in vertebrates through multiple mechanisms, such as complimentary base pairing with the 3′-UTR of their target mRNAs, resulting in translational repression, mRNA cleavage and mRNA decay initiated by miRNA-guided rapid deadenylation ( 10 ). (spandidos-publications.com)
  • The fact that and are up-regulated in HCC led us to hypothesize that normal and expressions are balanced by endogenous miR-31, which selectively regulates and mRNA translation in normal hepatic liver cells. (nos-nop.org)
  • MicroRNAs (miRNAs) are regulatory small non-coding RNAs that can regulate gene expression by binding to gene elements, such as the gene promotor 5?UTR, mainly in the 3?UTR of mRNA. (molcells.org)
  • MicroRNAs (miRNAs) are a kind of small noncoding RNAs that regulate the expression of protein through targeting the 3′UTR of messenger RNA (mRNA), resulting in mRNA degradation or suppression of translation [ 5 ]. (degruyter.com)
  • Mature microRNAs (miRNAs) are 21 to 23 nucleotide noncoding RNA molecules that can downregulate multiple gene expression by mRNA degradation or translational repression. (oncotarget.com)
  • A first RNA molecule comprises a double-stranded sequence, which includes a guide strand sequence that is complementary to a portion of an mRNA transcript encoded by the target gene. (genomeweb.com)
  • Preferably, the second guide strand sequence comprises one or more bases that are mismatched with a nucleic acid sequence of the mRNA transcript encoded by the target gene. (genomeweb.com)
  • A reduction in secreted reporter protein activity relative to a control cell into which the test polynucleotide has not been introduced is indicative that the test polynucleotide is capable of reducing the level of the target mRNA in the cells. (genomeweb.com)
  • The seed region in miRNAs, approximately six nucleotides long, recognizes and binds to the complementary binding site of the target mRNA 3'untranslated region (3'UTR) to regulate its expression. (molcells.org)
  • These naturally occurring, noncoding, single-stranded RNAs (21-25 nucleotides long) base-pair with their target mRNA within the RISC (RNA-induced silencing complex). (genengnews.com)
  • Recently, miRNA-messenger RNA (mRNA) regulatory relationships have been confirmed during biological processes, including osteogenic differentiation. (frontiersin.org)
  • This study aimed to find out more candidate miRNA-mRNA pairs involved in the osteogenic differentiation of MC3T3-E1 cells. (frontiersin.org)
  • Multiple miRNA-mRNA prediction databases were searched by differentially expressed genes (DEGs) to obtain pairs of a miRNA-DEG regulatory network. (frontiersin.org)
  • The miRNA-mRNA interactions were verified by dual-luciferase reporter gene assays and experiments using mimics miRNA or their inhibitors. (frontiersin.org)
  • There were 7 overlapped miRNA-mRNA pairs identified during osteogenic differentiation of MC3T3-E1 cells, including mmu-miR-204-5p-Arhgap11a, mmu-miR-211-5p-Arhgap11a, mmu-miR-24-3p-H2afx, mmu-miR-3470b-Chek2, mmu-miR-3470b-Dlgap5, mmu-miR-466b-3p-Chek1, and mmu-miR-466c-3p-Chek1. (frontiersin.org)
  • The Long Intergenic Noncoding RNA UFC1, a Target of microRNA 34a, Interacts with the mRNA Stabilizing Protein HuR to Increase Levels of β-catenin in HCC Cells. (aksomics.com)
  • LncRNA and mRNA interaction study based on transcriptome profiles reveals potential core genes in the pathogenesis of human glioblastoma multiforme. (aksomics.com)
  • The Role of PinX1 in Growth Control of Breast Cancer Cells and Its Potential Molecular Mechanism by mRNA and lncRNA Expression Profiles Screening. (aksomics.com)
  • Gene set variation analysis (GSVA) was used to establish and validate a miRNA set-based pathway prognostic signature for PC (miPPSPC) and a mRNA set-based pathway prognostic signature for PC (mPPSPC) in independent datasets. (aging-us.com)
  • 18 DERs and DEDs were identified in the PTM vs. PM, 87 in the TM vs. PTM, and 87 in the TM vs. PM. Those DERs and DEDs participated in the regulation of gene expression at the levels of chromatin conformation, gene activation and silencing, splicing and degradation of mRNA, biogenesis of piRNA and miRNA, ribosome assemble, and translation of proteins. (peerj.com)
  • This regulation included whole process of biological genetic information transmission from chromatin conformation to gene activation and silencing to mRNA splicing to ribosome assemble to translation of proteins and degradation of mRNA. (peerj.com)
  • Furthermore, miR-124-3p directly targets mRNA 3'UTR region of BCAT1, which results in upregulation of BCAT1 expression as observed in ESCC tissues and cell lines. (biomedcentral.com)
  • Pansensitive and panresistant genes to 21 NCCN-recommended drugs with concordant mRNA and protein expression were identified. (cdc.gov)
  • MiR-222-3p was confirmed to exhibit higher expression level in oral squamous cell carcinoma (OSCC). (biovendor.com)
  • Long Non-Coding RNA Profiling in Laryngeal Squamous Cell Carcinoma and Its Clinical Significance: Potential Biomarkers for LSCC. (aksomics.com)
  • Abstract Oral squamous cell carcinoma (OSCC) is a malignant tumor triggered by the accumulation of multiple gene mutations in oral epithelial cells. (techscience.com)
  • Esophageal squamous cell carcinoma (ESCC) is the major subtype of esophageal cancer with high aggressiveness and poor prognosis. (biomedcentral.com)
  • Esophageal squamous cell carcinoma (ESCC) is the major subtype of esophageal cancer and is highly prevalent in China [ 2 ]. (biomedcentral.com)
  • Recently, an increasing number of reports have implicated a new class of small regulatory RNA molecules, termed microRNAs (miRNAs), in HCC progression. (spandidos-publications.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • In the case of RNA molecules that bind target RNA segments, such as microRNAs (miRNAs), the altered base pairing can change binding specificities. (biomedcentral.com)
  • NcRNAs are a class of RNA molecules that do not encode proteins, and they play an important role in regulating cellular transcription and post-transcriptional processes [ 7 ]. (ijbs.com)
  • MicroRNAs (miRNAs) are 21-23 nucleotide RNA molecules that modulate the stability or translational efficiency of target messenger RNAs [ 1 , 2 ]. (oncotarget.com)
  • Such methods generally comprise providing a cell with one or more precursor nucleic acid sequences that encode two or more RNA molecules. (genomeweb.com)
  • MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. (biovendor.com)
  • MicroRNAs are regulatory molecules and suggested as non-invasive biomarkers for molecular diagnostics and prognostics. (biomedcentral.com)
  • Chemical modifications to provide drug-like properties to RNA molecules are used in the synthesis of both siRNAs and antimicroRNAs (antimiRs). (genengnews.com)
  • A growing body of evidence indicates that epigenetic regulators rely, amongst other factors, on their interaction with untranslated RNA molecules for guidance to particular targets on DNA. (frontiersin.org)
  • Non (protein)-coding RNAs are the most abundant transcriptional products of the coding genome, and comprise several different classes of molecules with unique lengths, conformations and targets. (frontiersin.org)
  • Our data indicate that extracellular ST6GAL1 from remote sources can compensate for cellular ST6GAL1-mediated aggressive tumor cell proliferation and invasive behavior and has great clinical potential for extracellular ST6GAL1 as these molecules are in the extracellular space should be easily accessible targets. (nature.com)
  • Recently, increasing evidences elucidated that miRNAs was involved in the cancer pathogenesis, including cell proliferation, metastasis, and apoptosis [ 6 ]. (hindawi.com)
  • Also, in gastric cancer, miR-455 inhibited human cell proliferation and invasion and promoted cell apoptosis [ 12 ]. (hindawi.com)
  • In addition, miR-455 inhibited cell viability, while it induced cell apoptosis in colorectal cancer [ 13 ]. (hindawi.com)
  • The progression of hepatocellular carcinogenesis is thought to involve the deregulation of genes that are critical to cellular processes, such as cell cycle control, cell growth, apoptosis, cell migration and spreading. (spandidos-publications.com)
  • Many of these miRNAs act as tumor suppressors and/or oncogenes, and are involved in cell activities that include development, differentiation, proliferation, apoptosis, metabolism and immunity ( 7 , 10 , 11 ). (spandidos-publications.com)
  • Induction of autophagy and apoptosis by miR-148a through the sonic hedgehog signaling pathway in hepatic stellate cells. (ajcr.us)
  • Annexin V-PE/PI and PI staining were used to analyze cell apoptosis and cell cycle distribution by flow cytometry, respectively. (biomedcentral.com)
  • Functional experiments demonstrated that knockdown of PTTG3P inhibited cell proliferation, migration and invasion, and promoted cell apoptosis, acting as an oncogene. (biomedcentral.com)
  • Cell apoptosis was analyzed by flow cytometry. (degruyter.com)
  • Moreover, TGFBR2 was a direct target of miR-128-3p, and its overexpression reversed the inhibitory effects of miR-128-3p overexpression on inflammation and apoptosis in LPS-induced HK2 cells. (degruyter.com)
  • miR-128-3p could inhibit apoptosis and inflammation by targeting TGFBR2 in LPS-induced HK2 cells, which might provide therapeutic strategy for the treatment of sepsis. (degruyter.com)
  • Transforming growth factor-β (TGF-β) signaling pathway is a multifunctional cytokine and plays important roles in regulating cell proliferation, cell cycle, differentiation, migration, and apoptosis in a broad spectrum of tissues [ 12 ]. (degruyter.com)
  • An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
  • Although KLF6 overexpression in human cancer cells inhibits cell proliferation, induces apoptosis, and attenuates cell migration, the effects of KLF6 on oral cancer remains poorly elucidated. (medsci.org)
  • KLFs regulate a wide range of cellular functions, including cell proliferation, apoptosis, differentiation, and neoplastic transformation, by binding to GC-rich promoter regions [ 1 , 2 ]. (medsci.org)
  • however, forced KLF6 expression in lung cancer cell lines can trigger cells to undergo apoptosis and reduce colony formation ability [ 6 ]. (medsci.org)
  • miRNAs are considered to play important roles in cell proliferation, apoptosis, and differentiation during mammalian development. (oncotarget.com)
  • Combination of miR-130a and miR-495 significantly decreased apoptosis determined by Annexin V-FITC/propidium iodide staining and flow cytometric analysis, and the expression of Bim in SNU484 gastric cancer cells. (oncotarget.com)
  • The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. (cancerindex.org)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • In the past few decades, studies have focused on investigating the genes and proteins responsible for the development and progression of HCC ( 3 ). (spandidos-publications.com)
  • Finally, IGF‑1R protein, phosphoinositide 3‑kinase/Akt signaling pathway‑associated proteins and cyclin pathway‑associated proteins were differentially expressed between miR‑497‑overexpressing cells and miR-497-silenced cells. (spandidos-publications.com)
  • metastatic potential of HCC cells by selectively regulating epithelial-mesenchymal transition (EMT) regulatory proteins such as N-cadherin, E-cadherin, vimentin and fibronectin. (nos-nop.org)
  • HCC tissues derived from chemical-induced rat liver cancer models validated that miR-31 manifestation is significantly down-regulated, and that those cell cycle- and EMT-regulatory proteins are deregulated in rat liver cancer. (nos-nop.org)
  • Overall, we suggest that miR-31 functions like a tumor suppressor by selectively regulating cell cycle and EMT regulatory proteins in human being hepatocarcinogenesis providing a novel target FANCG for the molecular treatment of liver malignancies. (nos-nop.org)
  • m 6 A is catalysed by RNA methyltransferases METTL3, METTL14 and WTAP (writers), m 6 A is removed by the demethylases FTO and ALKBH5 (erasers) and interacts with m6A-binding proteins, such as YT521-B homology (YTH) domain-containing proteins. (biomedcentral.com)
  • This study aimed to evaluate the expression levels of miR-34a and 11 of its bioinformatically selected target genes and proteins to test their potential dysregulation in RCC. (hindawi.com)
  • Krüppel-like factors (KLFs) are highly conserved zinc-finger proteins that regulate cellular transcription machinery [ 1 , 2 ]. (medsci.org)
  • Previously, non-coding RNAs (ncRNAs), including micro-RNAs (miRNAs) and other long non-coding RNAs (lncRNAs), have been generally considered unable to encode proteins both in plants and animals [1-9]. (fortunepublish.com)
  • This study is to explore the biogenesis mechanism of a secondary hepatocellular carcinoma (HCC) based on the function of RNA binding proteins (RBPs)-encoding genes in the physiological microenvironment (PM). (peerj.com)
  • The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)
  • (B) effect of miR-503 on the expression of endogenous genes. (biomedcentral.com)
  • MicroRNAs (miRNAs) are small, endogenous, non-coding RNAs, which act as key post-transcriptional regulators of target-gene expression. (spandidos-publications.com)
  • Mechanistically, PTTG3P upregulated the expression of miR-383 targets Cyclin D1 (CCND1) and poly ADP-ribose polymerase 2 (PARP2) by sponging miR-383, acting as a competing endogenous RNA (ceRNA). (biomedcentral.com)
  • A series of studies indicate that lncRNAs serve as competing endogenous RNAs (ceRNA) by sponging miRNAs, and modulate the targets of miRNAs [ 15 , 16 ]. (biomedcentral.com)
  • A long noncoding RNA critically regulates Bcr-Abl-mediated cellular transformation by acting as a competitive endogenous RNA. (aksomics.com)
  • However, it is still enigmatic about the exact processes in which RBPs-encoding genes and the relevant genes participate in transcriptome level during the biogenesis of secondary tumor in the activity of hepatocellular carcinoma (HCC) cells. (peerj.com)
  • The long non-coding RNA lnc-ZNF180-2 is a prognostic biomarker in patients with clear cell renal cell carcinoma. (ajcr.us)
  • Renal cell carcinoma (RCC) incidence has increased over the past two decades. (hindawi.com)
  • Renal cell carcinoma (RCC) accounts for approximately 3% of human malignancies, and its incidence appears to be increasing globally [ 1 ]. (hindawi.com)
  • Expression Pattern of Long Non-coding RNAs in Renal Cell Carcinoma Revealed by Microarray. (aksomics.com)
  • Similarly, the miRNA signature shows predictive value in adults with T-cell lymphoblastic lymphoma [ 9 ], and long noncoding RNA shows predictive value in adults with localized clear cell renal cell carcinoma [ 10 ]. (aging-us.com)
  • The relationship between blood pressure and risk of renal cell carcinoma. (who.int)
  • Epidemiology of Renal Cell Carcinoma: 2022 Update. (who.int)
  • Mycotoxin Exposure and Renal Cell Carcinoma Risk: An Association Study in the EPIC European Cohort. (who.int)
  • Biomarkers of the transsulfuration pathway and risk of renal cell carcinoma in the European Prospective Investigation into Cancer and Nutrition (EPIC) study. (who.int)
  • Importantly, we demonstrate that the overexpression of miR-148a significantly inhibits HCC cell proliferation and cell cycle progression. (spandidos-publications.com)
  • This miRNA functions as a tumor suppressor and dysregulation or loss of the host gene from which this miRNA is processed is associated with cancer progression in numerous cell types. (cancerindex.org)
  • Up-regulated CKS2 promotes tumor progression and predicts a poor prognosis in human colorectal cancer. (ajcr.us)
  • Up regulation of the long non-coding RNA NEAT1 promotes esophageal squamous cell carcinomacell progression and correlates with poor prognosis. (ajcr.us)
  • Emerging evidence indicates that Long non-coding RNAs (LncRNAs) and microRNAs (miRNAs) play crucial roles in tumor progression, including hepatocellular carcinoma (HCC). (biomedcentral.com)
  • These findings indicate miR-34a along with its putative target genes could play a role in RCC tumorigenesis and progression. (hindawi.com)
  • The loss of KLF6 expression is also correlated with cancer progression, tumor recurrence, and short survival time in head and neck carcinomas [ 7 ]. (medsci.org)
  • LncRNA AGPG has been shown to play a role in esophageal cancer by binding to and stabilizing PFKB3, which increases glycolytic flux and cell cycle progression in esophageal cancer cells. (ijbs.com)
  • Feng, Y Abstract: BACKGROUND: Accumulating studies have indicated the involvement of microRNAs (miRNAs) in the progression and treatment of human hepatocellular carcinoma (HCC). (hku.hk)
  • It is within the cell-autonomous context that ST6GAL1 involvement in cancer progression has been interpreted. (nature.com)
  • Cancer cell-extrinsic mechanisms, which are poorly understood, are also believed to contribute to disease progression and the heterogeneous genetic mutations with diverse presentations. (nature.com)
  • Our study thus clarifies a new mechanism that DNMT1/miR-124/BCAT1 axis regulates the development and progression of ESCC. (biomedcentral.com)
  • However, it is lack of evidence that these genes are essentially associated with ESCC progression. (biomedcentral.com)
  • These findings prompted us to investigate the role of DNMT/miR-124/BCAT1 axis in regulating development and progression of ESCC. (biomedcentral.com)
  • We previously identified differentially expressed microRNAs with liver-specific target genes in plasma from children with chronic hepatitis B. To further understand the biological role of these microRNAs in the pathogenesis of chronic hepatitis B, we have used the human liver cell line HepG2, with and without HBV replication, after transfection of hepatitis B virus expression vectors. (biomedcentral.com)
  • Identifcation of differentially expressed long non-coding RNAs in CD4+ T cells response to latent tuberculosis infection. (aksomics.com)
  • The differentially expressed RBPs-encoding genes (DERs) and differentially expressed DERs-associated genes (DEDs) were screened to undergo GO and KEGG analysis. (peerj.com)
  • microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (nih.gov)
  • Herein, we describe the epigenetic regulation of miR-148a and its impact on HCC cells. (spandidos-publications.com)
  • In addition to DNA and histone methylation, another level of epigenetic regulation, namely, RNA methylation, has become a hot topic in biosciences over the past decade. (biomedcentral.com)
  • Despite the well-known fact that primiRNAs are acting as non-protein-coding RNAs, the published data suggest that, in the plant genomes, some pri-miRNAs can also be found in polysomes, and the expression of these miRNA precursors may results in formation of micropeptides which may be involved in regulation of gene expression. (fortunepublish.com)
  • MicroRNAs mediate regulation of chemoresistance of hepatocellular carcinoma cells with involvement of p53 tumour suppressor. (hku.hk)
  • In recent years, emerging studies have highlighted the critical role of these pathways and their regulation by microRNAs (miRNAs) in cancer invasion and metastasis. (intechopen.com)
  • Murine hepatic miRNAs expression and regulation of gene expression in diet-induced obese mice. (genosensor.co)
  • Even small changes in microRNA expression may therefore have implications for gene regulation in various physiological and pathophysiological states [ 1 , 6 ]. (biomedcentral.com)
  • Osteoblast mainly originates from mesenchymal stem cells (MSCs), and it is involved in bone formation, remodeling, and reconstruction via the formation of a bone matrix and regulation of bone resorption ( 6 ). (frontiersin.org)
  • In particular, there are incremental examples of the participation of the distinct class of long non-coding RNAs (lncRNAs) in AML epigenetic regulation, some of which have translational potential. (frontiersin.org)
  • On the other hand, we clarified the upstream mechanism regulating miR-124-3p expression in ESCC, which involves in the hypermethylation-silencing regulation mediated by DNA methyltransferase 1(DNMT1), which is of high expression in ESCC tissues and cell lines in the present study. (biomedcentral.com)
  • DNA methylation involves in the addition of a methyl (CH3) group to DNA with DNA methyltransferases (DNMTs), thereby often modifying gene function through regulation of gene expression [ 7 ]. (biomedcentral.com)
  • A microRNA is a small non-coding RNA molecule, which functions in the post-transcriptional regulation of gene expression by partially complementing with the 3′-untranslated regions (3'UTR) of specific mRNAs [ 14 ]. (biomedcentral.com)
  • In this review, we focus on the relationship between RNA m 6 A methylation and gastrointestinal cancer, especially their role, mechanism and potential clinical application as biomarkers and therapeutic targets for gastrointestinal cancer. (biomedcentral.com)
  • Recent studies reported microRNAs as promising biomarkers for early cancer detection, accurate prognosis, and molecular targets for future treatment. (hindawi.com)
  • miR-1254 and miR-574-5p: serum-based microRNA biomarkers for early-stage non-small cell lung cancer. (genosensor.co)
  • MicroRNAs are suggested as non-invasive biomarkers for molecular diagnostics and prognostics, and some microRNAs show promising results as therapeutic targets in human trials [ 1 - 3 ]. (biomedcentral.com)
  • Abstract Circulating tumor cells (CTCs) are essential biomarkers for liquid biopsies, which are important in the early screening, prognosis, and real-time monitoring of cancer. (techscience.com)
  • The invention comprises "a method of diagnosing, assessing susceptibility, and/or treating schizophrenia involving the identification and/or observation of microRNAs and variant miRNA," according to the patent's abstract. (genomeweb.com)
  • The invention relates to a method for inhibiting the expression of a target gene in a eukaryotic cell," the patent's abstract states. (genomeweb.com)
  • The invention comprises "methods and compositions for the diagnosis, prognosis, and treatment of hepatocellular carcinoma … [as well as] methods of identifying [anti-cancer] agents," the patent's abstract states. (genomeweb.com)
  • The patent, its abstract states, relates to "duplex oligonucleotide complexes [that] can be administered to a cell, tissue or organism to silence a target gene without the aid of a transfection reagent. (genomeweb.com)
  • Hepatocellular carcinoma (HCC) has been regarded as the fifth most common cancer worldwide with a low prognosis. (hindawi.com)
  • Methylation associated genes contribute to the favorable prognosis of gliomas with isocitrate dehydrogenase 1 mutation. (ajcr.us)
  • Long noncoding RNA GAPLINC regulates CD44-dependent cell invasiveness and associates with poor prognosis of gastric cancer. (aksomics.com)
  • Aggressive breast cancer is a highly heterogeneous disease caused by a variety of distinct cell-intrinsic genetic alterations in mammary epithelial cells, leading to vastly heterogenic disease manifestation in individual patients and predominantly affecting patient prognosis and treatment options [ 14 ]. (nature.com)
  • Most of the existing research has focused on genes for prognosis prediction, whereas pathways based on mRNAs or miRNAs have never been used to predict survival. (aging-us.com)
  • Moreover, MGO inhibits AMPK activity, reduces LC3II accumulation, and suppresses protein and gene expressions of MFN1, PGC-1α and TFAM, leading to mitochondrial fission, inhibition of mitochondrial biogenesis and autophagy. (bvsalud.org)
  • MicroRNA-320a inhibits proliferation and invasion of breast cancer cells by targeting RAB11A. (ajcr.us)
  • For example, a small peptide that is encoded by lncRNA HOXB-AS3 inhibits oncogenesis by regulating alternative splicing and metabolic reprogramming of colon cancer cells [5-7, 17]. (fortunepublish.com)
  • MicroRNA-29b-2-5p inhibits cell proliferation by directly targeting Cbl-b in pancreatic ductal adenocarcinoma. (genosensor.co)
  • MicroRNAs (miRNAs) are a class of small non-coding RNA, 20-22 nucleotides in length. (molcells.org)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
  • Krüppel-like factors can bind to specific DNA motifs and regulate various cellular functions, such as metabolism, cell proliferation, and differentiation. (medsci.org)
  • Since then, several microproteins have been functionally characterized, which may act, for example, as signals promoting cell migration and differentiation of human cells [5, 7]. (fortunepublish.com)
  • An MC3T3-E1-based microarray dataset (accessioned as GSE46400) downloaded from the Gene Expression Omnibus included MC3T3-E1 cells with or without 14-day osteoblast differentiation osteoblast induction. (frontiersin.org)
  • The MC3T3-E1 cells were cultured and incubated in the osteogenic differentiation medium for 14 days. (frontiersin.org)
  • The Arhgap11a, H2afx, Chek2, Dlgap5, and Chek1 were hub genes downregulated in MC3T3-E1 cells after osteogenic differentiation, verified by RT-qPCR results. (frontiersin.org)
  • Our study characterizes miR-211-5p targeting Arhgap11a promotes the osteogenic differentiation of MC3T3-E1 cells, which provides novel targets to promote the osteogenesis process during bone repair. (frontiersin.org)
  • Akin to the normal hematopoietic system, leukemias are sustained by a small number of leukemia stem-like cells (LSC), which can be distinct from the normal hematopoietic stem cells (HSC) but also exhibit functional characteristics of self-renewal and (abnormal or hindered) differentiation, and are often quiescent ( 2 - 4 ). (frontiersin.org)
  • We found that, due to the hypermethylation of its CpG island, miR-148a undergoes methylation-mediated silencing in HCC cell lines. (spandidos-publications.com)
  • Additionally, DNMT1, the DNA methyltransferase that maintains methylation patterns, is aberrantly upregulated in HCC cell lines, and its overexpression is responsible for hypermethylation of the miR-148a promoter. (spandidos-publications.com)
  • Epigenetic alternations in genomic DNA include cytosine methylation in CpG islands, which usually extend throughout the promoters and the first exons of genes. (spandidos-publications.com)
  • DNA methylation, which is associated with gene silencing ( 12 ), is carried out by DNA methyltransferases (DNMTs). (spandidos-publications.com)
  • Recent studies have established that, similar to mutations, methylation-mediated silencing of tumor suppressor genes plays a major role in tumorigenesis. (spandidos-publications.com)
  • Analogous to DNA methylation and histone modification, RNA modification, as another epigenetic layer, plays an important role in many diseases, especially in tumours. (biomedcentral.com)
  • As the most common form of RNA modification, m 6 A methylation has attracted increasing research interest in recent years. (biomedcentral.com)
  • Although the full scope of epigenetics has not yet been determined, it is generally defined as chemical modification that mainly includes DNA and RNA methylation, histone modification, noncoding RNA modification and chromatin rearrangement. (biomedcentral.com)
  • For example, 5-methylcytosine methylation in DNA has affected gene expression in many tumours. (biomedcentral.com)
  • Common RNA methylation sites include 5-methylcytosine (m 5 C), 7-methylguanosine (m 7 G), m 1 G, m 2 G, m 6 G, N 1 -methyladenosine (m 1 A) and m 6 A. m 5 C modification promotes splicing and translation [ 4 ]. (biomedcentral.com)
  • LIM-only protein 3 (LMO3), a member of the LIM-only protein group, is a new DNA methylation gene that was identified in gliomas via the MeDIP-Chip in our previous study. (oncotarget.com)
  • They bind primarily to 3′-untranslated regions (3′-UTRs) of target gene mRNAs. (spandidos-publications.com)
  • One miRNA targets many mRNAs, which can be regulated by many miRNAs, leading to a complex metabolic network. (molcells.org)
  • Transcriptome-wide research reveals that m 6 A modification may affect more than 7000 mRNAs in individual transcriptomes of mammalian cells. (biomedcentral.com)
  • Although RNA editing has long been considered a relatively rare processing event, more recent research suggests that the vast majority of pre-mRNAs are edited [ 6 ]. (biomedcentral.com)
  • MiRNAs, short (18-24 nucleotides) non-coding RNAs, are derived from long transcripts pri-miRNAs and pre-miRNAs [ 26 - 30 ].By targeting 3' untranslated regions (3'UTRs) of cognate mRNAs, miRNAs post-transcriptionally regulate gene expression and induce translational repression [ 29 , 30 ]. (intechopen.com)
  • It's been suggested that miRNAs regulate up to 50% of all mRNAs in the human genome. (genengnews.com)
  • The expressions of candidate miRNAs and mRNAs were determined by real-time quantitative PCR(RT-qPCR) in MC3T3-E1 cells. (frontiersin.org)
  • MicroRNAs (miRNAs) are small, noncoding RNAs that bind to the 3' UTR of target mRNAs, and silence gene expression by inducing degradation of target mRNAs or inhibition of protein translation [ 10 ]. (cdc.gov)
  • However, miR-455 promoted cell invasion and migration in triple-negative breast cancer [ 14 ]. (hindawi.com)
  • We also showed that expression of TGF-beta RII, which has been regarded as a regulator of tumor proliferation, invasion, and migration in hepatocellular carcinoma, is regulated by miRNA-590-5p. (molcells.org)
  • Muc1 promotes migration and lung metastasis of melanoma cells. (ajcr.us)
  • Selective inhibition of EGFR downstream signaling reverses the irradiation-enhanced migration of HNSCC cells. (ajcr.us)
  • microRNA-506 regulates proliferation, migration and invasion in hepatocellular carcinoma by targeting F-spondin 1 (SPON1). (ajcr.us)
  • Transwell migration and invasion assays were used to examine cell migration and invasion abilities. (biomedcentral.com)
  • KLF6 overexpression attenuated the migration and invasion of oral cancer SAS cells. (medsci.org)
  • MicroRNA-328 is associated with (non-small) cell lung cancer (NSCLC) brain metastasis and mediates NSCLC migration. (genosensor.co)
  • We show downregulation of miR-124-3p expression in ESCC tissues, which is highly correlated with proliferation and migration of ESCC cell lines KYSE-150 and Eca109. (biomedcentral.com)
  • Existing studies have shown that there is crossover between Renin-Angiotensin-Aldosterone (RAAS) signaling pathway and TGF-β pathway, but little is known about whether ARB drug losartan can block Angiotensin-II (Ang-II) signaling pathway, inhibit the activation of Transforming Growth Factor- Beta (TGF-β) pathway, and regulate vascular remodeling. (ijpsonline.com)
  • Mechanism of miR-455-3 in suppressing epithelial-mesenchymal transition and angiogenesis of non-small cell lung cancer cells. (nih.gov)
  • MiR-449a suppresses cell invasion by inhibiting MAP2K1 in non-small cell lung cancer. (ajcr.us)
  • These results were then confirmed in glioblastomas, in thyroid papillary carcinomas, in breast cancer, hepatocellular carcinoma, and lung cancer. (biovendor.com)
  • Gene expression-based prognostic signatures have a significant effect on predicting the survival of patients with malignant tumors, such as non-small cell lung cancer [ 7 ] and pediatric acute myeloid leukemia [ 8 ]. (aging-us.com)
  • Simple Summary: This study identified a set of 73 microRNAs (miRNAs) that can accurately detect lung cancer tumors from normal lung tissues. (cdc.gov)
  • From the list of genes that were targeted by the 73 diagnostic miRNAs, DGKE and WDR47 had significant associations with responses to both systemic therapies and radiotherapy in lung cancer. (cdc.gov)
  • Based on our identified miRNA-regulated network, we discovered three drugsBX-912, daunorubicin, and midostaurinthat can be repositioned to treat lung cancer, which was not known before. (cdc.gov)
  • This study identified a set of 73 microRNAs (miRNAs) that classified lung cancer tumors from normal lung tissues with an overall accuracy of 96.3% in the training patient cohort (n = 109) and 91.7% in unsupervised classification and 92.3% in supervised classification in the validation set (n = 375). (cdc.gov)
  • Based on our identified miRNA-regulated molecular machinery, an inhibitor of PDK1/Akt BX-912, an anthracycline antibiotic daunorubicin, and a multi-targeted protein kinase inhibitor midostaurin were discovered as potential repositioning drugs for treating lung cancer. (cdc.gov)
  • Cell viability and invasion were measured by CCK8 and Transwell assays. (hindawi.com)
  • miR-455 suppressed cell proliferation in vitro and in vivo, and it inhibited the abilities of cell invasion and EMT in HCC. (hindawi.com)
  • RAB18 reversed partial roles of miR-455 on cell viability and invasion in HCC. (hindawi.com)
  • Downregulation of miR-590-5p inhibited proliferation and invasion of hepatocellular carcinoma cells (HCCs). (molcells.org)
  • We also found that downregulation of miR-590-5p was associated with an elevation of TGF-beta RII and inhibition of proliferation and invasion in HepG2 cells. (molcells.org)
  • Furthermore, overex-pression of miR-590-5p was associated with upregulation of TGF-beta RII and could promote proliferation and invasion in L02 cells. (molcells.org)
  • Thus, the role of TGF-beta RII in regulating proliferation and invasion of human HCCs is controlled by miR-590-5p. (molcells.org)
  • LIN28B suppresses microRNA let-7b expression to promote CD44+/LIN28B+ human pancreatic cancer stem cell proliferation and invasion. (ajcr.us)
  • Moreover, inhibition of miR-222-3p can suppress OS cell proliferation, colony formation, and invasion in vitro. (biovendor.com)
  • Long Noncoding RNA Activated by TGF-β Promotes the Invasion-Metastasis Cascade in Hepatocellular Carcinoma. (aksomics.com)
  • A novel long non-coding RNA ENST00000480739 suppresses tumour cell invasion by regulating OS-9 and HIF-1α in pancreatic ductal adenocarcinoma. (aksomics.com)
  • Tumor-native ST6GAL1 promotes tumor cell behaviors such as invasion and resistance to cell stress and chemo- and radio-treatments. (nature.com)
  • The abnormality of those functions in the organic microenvironments promoted the metastasis of HCC and initiated the biogenesis of a secondary HCC in a PM when the PM encountered the invasion of cancer cells. (peerj.com)
  • With the study of loss-of-function and gain-of-function in DNMT/miR-124/BCAT1, we showed that downregulation of hsa-miR-124-3p in ESCC tissues and cells contributed to the upregulation of BCAT1 expression, which enhanced the proliferation and invasion of ESCC. (biomedcentral.com)
  • This class of noncoding RNAs is small, single stranded, and 19-25 nucleotide long that act as negative regulators involved in posttranscriptional silencing of the gene expression [ 5 ]. (hindawi.com)
  • ADAR3 contains an arginine-rich domain, shown in pink, which binds single-stranded RNA. (biomedcentral.com)
  • MiRNAs are short, single-stranded non-coding RNAs that exist in organisms widely and can regulate a lot of pathological and physiological processes. (zhqkyx.net)
  • To date, 1527 human miRNAs have been identified (Sanger miRBase 18 http://www.miRbase.org/index.shtml), forming less than 1% of all human genes, potentially regulating more than 10% of all protein coding genes [ 1 ]. (intechopen.com)
  • Twenty-seven of these substitution mutations have been observed in genes encoding viral spike protein (S), although most differences were found in non-structural protein-coding genes. (molcells.org)
  • A-to-I editing is a mechanism that regulates and diversifies the transcriptome, but the full biological significance of ADARs is not understood. (biomedcentral.com)
  • Eukaryotic RNA transcripts can undergo a range of post-transcriptional modifications, which increase the diversity of the transcriptome without requiring increases in genome size. (biomedcentral.com)
  • For the current study, we performed transcriptome and small RNA sequencing to profile gene expression in SB-induced HCCs in an attempt to clarify the genetic element(s) contributing to tumorigenesis. (prolekarniky.cz)
  • In general, microRNAs, a class of small (~21 nucleotide) non-coding RNAs, negatively regulate the expression of their target genes. (spandidos-publications.com)
  • We performed a large case-control study involving 414 patients and 1199 cancer-free controls to investigate whether single nucleotide polymorphisms (SNPs) in the WDR4 gene are associated with Wilms tumor susceptibility. (jcancer.org)
  • RNA editing refers to post-transcriptional processes that alter the nucleotide sequence of an RNA transcript by insertion, deletion or nucleotide conversion. (biomedcentral.com)
  • (C) signaling pathways that regulate the cell cycle control in HCC involving miR-503. (biomedcentral.com)
  • A single microRNA can have multiple downstream targets and affect a number of different networks and pathways [ 4 , 5 ]. (biomedcentral.com)
  • LncRNA-regulated infection and inflammation pathways associated with pregnancy loss: Genome wide differential expression of lncRNAs in early spontaneous abortion. (aksomics.com)
  • In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)
  • As a result, ADAR-mediated editing can post-transcriptionally alter codons, introduce or remove splice sites, or affect the base pairing of the RNA molecule with itself or with other RNAs. (biomedcentral.com)
  • In Trp53KO/C-MycOE mice and mice with tumors grown from Hepa1-6 cells, injection of the combination of anti-PD1 and anti-TIGIT significantly reduced tumor growth, increased the ratio of cytotoxic to regulatory T cells in tumors, and prolonged survival.PVRL1, which is up-regulated by HCC cells, stabilizes cell surface PVR, which interacts with TIGIT, an inhibitory molecule on CD8+ effector memory T cells. (stanford.edu)
  • Our results suggest the existence of a novel miR-148a-DNMT1 regulatory circuit and indicate that miR-148a acts as a tumor suppressor during hepatocellular carcinogenesis. (spandidos-publications.com)
  • ADARs have been shown to contribute to disease pathologies by editing of glutamate receptors, editing of serotonin receptors, mutations in ADAR genes, and by other mechanisms, including recently identified regulatory roles in microRNA processing. (biomedcentral.com)
  • They use their complex epigenetic regulatory mechanisms to act as upstream regulators of downstream oncogenes or tumor suppressor genes [ 10 ]. (ijbs.com)
  • NRAV, a Long Noncoding RNA, Modulates Antiviral Responses through Suppression of Interferon-Stimulated Gene Transcription. (aksomics.com)
  • The Long Noncoding RNA Expression Profile of Hepatocellular Carcinoma Identified by Microarray Analysis. (aksomics.com)
  • Genome-wide analysis of long noncoding RNA in primary nasopharyngeal carcinoma by microarray. (aksomics.com)
  • Pseudogene, a subclass of long non-coding RNAs (lncRNAs), are considered as genomic loci that resemble real gene, but lost some functionality because they are lack of protein-coding ability because of disabling mutation, lack of transcription, or their inability to encode RNA [ 3 ]. (biomedcentral.com)
  • The aim of this mini-review is to discuss emerging epigenetic roles of long non-coding RNAs (lncRNAs) in Acute Myeloid Leukemia (AML). (frontiersin.org)
  • Exploring the Wnt Pathway-Associated LncRNAs and Genes Involved in Pancreatic Carcinogenesis. (aksomics.com)
  • MiR-222 and miR-221 are two highly homologous microRNAs located in tandem on the X chromosome in human, mouse and rat and are highly conserved in vertebrates. (biovendor.com)
  • Thus, the pivotal roles of miR-455 in cell viability and metastasis in HCC still needed to be explored. (hindawi.com)
  • m 6 A is the most common among various RNA modifications [ 9 ] and has critical roles in cancer pathogenesis. (biomedcentral.com)
  • The RNAs play important roles in the pathological and physiological tissues including cancer. (peerj.com)
  • Among of these genes, miR-124 caught our attention in view of the finding that large number of microRNAs(MiRNAs) have been shown important roles in the development of different cancers [ 11 ]. (biomedcentral.com)
  • 48 h after transfection with the indicated RNA duplex, cells were subjected to Western blot analysis. (biomedcentral.com)
  • As demonstrated in Fig. ?Fig.2B,2B, knockdown augmented HDAC2 and CDK2 protein expressions in SNU-449 and SKHep-1 cells, whereas co-transfection of miR-31 mimics attenuated knockdown effect on the same cells. (nos-nop.org)
  • The present study was conducted to identify suitable RGs for microRNA expression studies in a human HCC-derived cell line (HepG2 tet-on), with and without HBV replication, after transfection of HBV expression vectors. (biomedcentral.com)
  • DEFB1 expression after transfection with two micro RNAs (miRNAs), hsa-miR-186-5p and hsa-miR-340-5p, provided evidence that DEFB1 expression could be modulated by these miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1. (cdc.gov)
  • The expression of miR-455 was lower in HCC tissues and cell lines than in nontumor tissues and normal cell line, and downregulation of miR-455 was connected with worse outcome of HCC patients. (hindawi.com)
  • RAB18 was upregulated in HCC tissues and cell lines, and the expression of RAB18 was regulated by miR-455. (hindawi.com)
  • All the fresh tissues were snap-frozen in liquid nitrogen and stored at −80°C until RNA extraction. (hindawi.com)
  • In this study, we found that PTTG3P was up-regulated in HCC tissues and cells. (biomedcentral.com)
  • In this study, we found that LIM-only protein 3 (LMO3) is hypomethylated and overexpressed in glioma cells and tissues. (oncotarget.com)
  • A-to-I editing, which is catalyzed by enzymes of the adenosine deaminase acting on RNA (ADAR) family, is most prevalent in the central nervous system (CNS) but occurs in many tissues [ 1 - 3 ]. (biomedcentral.com)
  • It has been demonstrated that miR-222-3p was upregulated in OS tissues and OS cell lines. (biovendor.com)
  • HCC tissues from patients had higher levels of PVRL1 messenger RNA and protein than nontumorous tissues. (stanford.edu)
  • The binding sites of miR-128-3p and TGFBR2 were predicted by Targetscan online software and confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. (degruyter.com)
  • MiR-455-5p upregulation in umbilical cord mesenchymal stem cells attenuates endometrial injury and promotes repair of damaged endometrium via Janus kinase/signal transducer and activator of transcription 3 signaling. (nih.gov)
  • Therefore, the present study investigated miR‑497 expression in HCC samples and HCC‑derived cell lines using reverse transcription‑quantitative polymerase chain reaction. (spandidos-publications.com)
  • Unlike sequence-specific transcription factors, epigenetic regulators do not necessarily bind DNA at consensus sequences, but still achieve reproducible target binding in a manner that is cell and maturation-type specific. (frontiersin.org)
  • Dynamic Transcription of Long Non-Coding RNA Genes during CD4+ T Cell Development and Activation. (aksomics.com)
  • It focuses on how precision oncology has shaped advances in the impact of non-coding RNAs in epigenetics and cancer, immunotherapy and tumor biology, and the clinical significance of various therapies in a range of cancers. (biomedcentral.com)
  • knockdown of Pvrl1 reduced cell-surface levels of PVR but not levels of Pvr messenger RNA. (stanford.edu)
  • Primary liver cancer mainly refers to hepatocellular carcinoma (HCC), cholangiocarcinoma and hepatic angiosarcoma. (spandidos-publications.com)
  • Notably, berberine activated a population with immune suppressive function, defined as granulocytic‐ myeloid‐derived suppressor cell (G‐MDSC)‐like population, in the liver of mice with alleviating alcohol‐induced hepatic injury. (hku.hk)
  • Hepatocellular carcinoma (HCC) is the most common primary cancer of the liver. (spandidos-publications.com)
  • The manifestation of miR-31 was normalized to U6 snRNA (*test) (D) The qRT-PCR analysis of miR-31 for hepatocellular carcinoma cell lines (n=7) and liver normal cell lines (n=2) (**test). (nos-nop.org)
  • A large number of studies have demonstrated that the synergistic collaboration of a number of microRNAs (miRNAs), their growth factors and their downstream agents is required for the initiation and completion of pathogenesis in the liver. (wjgnet.com)
  • Berberine remarkably enhanced the increase of G‐MDSC‐like cells in blood and liver and decreased cytotoxic T cells correspondingly. (hku.hk)
  • Instead we use an in vitro HBV-replicating liver cell model to investigate the biological role of microRNAs with a possible influence on the pathogenesis of CHB in children. (biomedcentral.com)
  • Our findings indicate that tumor associated macrophages including Kupffer cells, have a profound impact on liver cancer and I am studying the molecular basis for these effects. (stanford.edu)
  • We investigated mechanisms of resistance of liver tumors in mice to infiltrating T cells.Mice were given hydrodynamic tail vein injections of clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR-Cas9) and transposon vectors to disrupt Trp53 and overexpress C-Myc (Trp53KO/C-MycOE mice). (stanford.edu)
  • Identification of miRNA-regulated targeting genes is important for understanding their specific biological functions [ 11 ]. (degruyter.com)
  • Immune checkpoint inhibitors are effective in the treatment of some hepatocellular carcinomas (HCCs), but these tumors do not always respond to inhibitors of programmed cell death 1 (PDCD1, also called PD1). (stanford.edu)
  • Mutations in this gene are associated with left ventricular noncompaction with congenital heart defects. (nih.gov)
  • This approach identified recurrent mutations within the Dlk1-Dio3 imprinted domain, indicating that alteration of one or more elements within the domain provides a selective advantage to cells during the process of hepatocarcinogenesis. (prolekarniky.cz)
  • Some of the same strategies already developed for delivery of siRNA for RNA interference (RNAi) also are being applied to miRNA. (genengnews.com)
  • Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)
  • Western blot analysis showed that ß-catenin (CTNNB1) signaling and genes involved in cellular proliferation were activated in BDL- and ANIT-treated mice. (bvsalud.org)
  • CCK-8 assay was performed to examine cell proliferation. (biomedcentral.com)
  • Luciferase reporter assay and RNA pull-down assay were carried out to detect the interaction between miR-383 and LncRNA PTTG3P. (biomedcentral.com)
  • prediction and identification of miRNA-targeted genes were conducted by TargetScan and luciferase reporter assay. (hku.hk)
  • HBEpCs overexpressing DEFB1 caused a significant reduction in IAV, that was confirmed by IAV matrix gene analysis, plaque assay, and confocal microscopy. (cdc.gov)
  • The small nuclear RNA U6 is frequently used as a reference gene (RG) in such studies [ 20 ]. (biomedcentral.com)
  • methods for reducing the expression level of a target gene. (genomeweb.com)
  • and introducing at least one siRNA that is complementary to the siRNA tag of the transfected genetic construct to inhibit the expression of the target gene. (genomeweb.com)
  • In the case of a GalNAc-conjugated siRNA targeting transthyretin (TTR), target gene silencing was achieved with an ED50 of approximately 5 mg/kg with a single subcutaneous injection. (genengnews.com)
  • These results represent a greater than 30-fold improvement in target gene silencing with siRNA conjugates as compared with first-generation siRNA conjugates previously described. (genengnews.com)
  • It reportedly delivers a greater than 30-fold improvement in target gene silencing. (genengnews.com)
  • miR-130a and miR-495 upregulated under hypoxic conditions that bind to the RUNX3 3′-untranslated region (3′-UTR) were identified in gastric cancer cells by using microarray analysis and bioinformatics programs. (oncotarget.com)
  • NcRNAs play a significant role in regulating gene expression in digestive system tumors. (ijbs.com)
  • Hepatocellular carcinoma (HCC) one of the most common digestive system tumors, threatens the tens of thousands of people with high morbidity and mortality world widely. (biomedcentral.com)
  • The duplex oligonucleotide complexes of the disclosure include a conjugate moiety that facilitates delivery to a cell, tissue, or organism. (genomeweb.com)
  • Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
  • Our study is the first to provide demonstrate that KLF6 functions as a tumor suppressor gene and prevents the metastasis of oral cancer cells. (medsci.org)
  • The tumor microenvironment (TM) in close contact with cancer cells is highly related to tumor growth and cancer metastasis. (peerj.com)
  • Thus, A-to-I editing in both translated and untranslated regions of RNA can be biologically significant. (biomedcentral.com)
  • The five yr Anisole Methoxybenzene survival rate was significantly decreased in patient with low level of miR-31 manifestation in the tumor cells (Log-rank = 0.0015*) (C) The qRT-PCR analysis for 9 paired HCC cells. (nos-nop.org)
  • In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that beta-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)