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Lactococcus lactisLactococcusNisinCheeseBacterial ProteinsMolecular Sequence DataBacteriophagesBacteriocinsGenes, BacterialSiphoviridaeGene Expression Regulation, BacterialLeuconostocLactobacillusBase SequenceDNA, BacterialFermentationPlasmidsAmino Acid SequenceKluyveromycesCloning, MolecularMilkOperonSequence Analysis, DNALactoseCultured Milk ProductsAcetoinConjugation, GeneticDiacetylOpen Reading FramesStreptococcus thermophilusFood MicrobiologyFood IndustryStreptococcus PhagesGenetic EngineeringCulture MediaPediococcusChromosomes, BacterialDairy ProductsPhosphofructokinasesRestriction MappingEscherichia coliSequence Homology, Amino AcidIndustrial MicrobiologyCholatesBacteriolysisAminopeptidasesLactobacillus plantarumCaseinsDNA Transposable ElementsTransformation, BacterialLactic AcidCell WallFood TechnologyStreptococcusPhosphoglucomutaseBifidobacteriumMicrococcal NucleaseL-Lactate DehydrogenaseLactobacillaceae
SubspCremorisBacillusStrainsGenomeBacteriaSpeciesProteinsNisinRecombinantOrganismStrainRRNA geneEnterococcusIsolatesBacteriocinLactic acidPlasmidBacteriumStreptococcusProteinAssaysColiPutativeDairy productsOperonsMotilityStructuralFunctionalSequenceMilkMainly isolatedCharacteristicsMotilePromoterPhylogeneticExperimentsGrowthSynthesisSalmonellaComparisonsTransportTransporter
Subsp7
Cremoris1
Bacillus7
  • Tandem genes encoding sigma-factors for consecutive steps of development in Bacillus subtilis. (microbiologyresearch.org)
  • Based on 16S rRNA gene sequences, histamine-forming bacteria were identified as Bacillus licheniformis MCH01, Serratia marcescens MCH02, Staphylococcus xylosus MCH03, Aeromonas hydrophila MCH04, and Morganella morganii MCH05. (e-fas.org)
  • A bacterial strain producing two antimicrobial peptides was isolated from a rhizosphere soil sample and identified as Bacillus subtilis based on both phenotypic and 16S rRNA gene sequence phylogenetic analysis. (springeropen.com)
  • The gene was transferred to Bacillus subtilis to investigate if the orotate transporter would be functional in this background. (biomedcentral.com)
  • Experiments in Bacillus subtilis using a plasmids with the ysbC gene show that transformants can not grow on minimal plates supplemented with Fluoro-orotate. (biomedcentral.com)
  • Further experiments presented on the poster show that the ysbC gene can be of use in general bacillus recombinant technology as an efficient counter selectable marker. (biomedcentral.com)
  • This work aimed to investigate the effect of dietary Lactococcus lactis and Bacillus subtilis on the immune responses, intestinal microbiota, and resistance to pathogens of Oscar, Astronotus ocellatus . (ij-aquaticbiology.com)
Strains7
  • Lactose fermentation In one study that sought to prove that some fermentation produced by L. lactis can hinder motility in pathogenic bacteria, the motilities of Pseudomonas, Vibrio, and Leptospira strains were severely disrupted by lactose utilization on the part of L. lactis. (wikipedia.org)
  • Electroporation was used to introduce genes for lactose utilization from Lactococcus lactis into strains of P. acidilactici and P. pentosaceus . (usu.edu)
  • The twenty-five LAB strains comprised eight strains belonging to Lactobacillus, four strains belonging to Lactococcus, eleven strains belonging to Enterococcus, and two strains belonging to Leuconostoc. (calbinotox.fr)
  • Lactococcus strains with or without the ysbC gene was tested for resistance against fluoro-orotate on minimal plates. (biomedcentral.com)
  • Only Lactococcus strains without the ysbC plasmid was able to grow on minimal plates with fluoro-orotate. (biomedcentral.com)
  • This Abi system exhibits activity against phages infecting Lb. brevis and Lactococcus lactis strains. (ucc.ie)
  • Restriction profiles obtained by digesting the genomic DNA with Sma I enabled differentiation of the reference strains of Lactococcus, Enterococcus, and Streptococus thermophilus. (iyte.edu.tr)
Genome3
  • in the genome of L. lactis MG1363. (wikipedia.org)
  • The encapsidated S RNA mimic (ESRM) was based on the plum pox virus (PPV) genome with the inserted targeted gene fragment. (bvsalud.org)
  • A putative gene, denoted ysbC , was previously identified by genome sequencing of a Lactococcus lactis strain, but the gene was not annotated in the databases, and no function of the predicted encoded polypeptide was identified. (biomedcentral.com)
Bacteria8
  • L. lactis is one of the best characterized low GC Gram positive bacteria with detailed knowledge on genetics, metabolism and biodiversity. (wikipedia.org)
  • Bacteriophages specific to L. lactis cause significant economic losses each year by preventing the bacteria from fully metabolizing the milk substrate. (wikipedia.org)
  • The region included a high proportion of genes that did not show homology with genes in other bacteria. (microbiologyresearch.org)
  • Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria. (lookformedical.com)
  • In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION. (lookformedical.com)
  • The objectives of this study were to identify the histamine-forming bacteria and bacteriocin- producing lactic acid bacteria (LAB) isolated from Myeolchi - jeot according to sequence analysis of the 16S rRNA gene, to evaluate the inhibitory effects of the bacteriocin on the growth and histamine accumulation of histamine-forming bacteria, and to assess the physico-chemical properties of the bacteriocin. (e-fas.org)
  • The results showed that adding B. subtilis and L. lactis , particularly in MIX 300 , reduced the anaerobic heterotrophic bacterial microbiota and increased lactic acid bacteria (LAB) in fish. (ij-aquaticbiology.com)
  • Some bacteria have the bioY gene clustering with bioB , which encodes biotin synthase, an enzyme that converts dethiobiotin to biotin. (tcdb.org)
Species2
Proteins7
  • Lactococcus lactis has been demonstrated to be a promising candidate for the delivery of functional proteins because of its noninvasive and nonpathogenic characteristics. (wikipedia.org)
  • Among the bacterial systems available, the Gram-positive lactic bacterium, Lactococcus lactis , traditionally used in food fermentations, is nowadays widely used for large-scale production and functional characterization of bacterial and eukaryotic membrane proteins. (springer.com)
  • The aim of this chapter is to describe the different possibilities for the functional characterization of peripheral or intrinsic membrane proteins expressed in Lactococcus lactis . (springer.com)
  • Kunji ERS, Slotboom DJ, Poolman B (2003) Lactococcus lactis as host for overproduction of functional membrane proteins. (springer.com)
  • Monné M, Chan KW, Slotboom DJ, Kunji ERS (2005) Functional expression of eukaryotic membrane proteins in Lactococcus lactis. (springer.com)
  • Bonds between fibronectin and fibronectin-binding proteins on Staphylococcus aureus and Lactococcus lactis. (duke.edu)
  • Phylogenetic comparisons indicated that the structural genes have considerable diversity and appear to have had a different pathway of evolution than the immunity proteins. (usda.gov)
Nisin4
  • Mierau I, Kleerebezem M (2005) 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. (springer.com)
  • de Ruyter PG, Kuipers OP, de Vos WM (1996) Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. (springer.com)
  • Zhou XX, Li WF, Ma GX, Pan YJ (2006) The nisin-controlled gene expression system: construction, application and improvements. (springer.com)
  • Only one bacteriocin (nisin from Lactococcus lactis) has been used commercially, but it has had a variety of applications (food safety and preservation, animal growth promotion, industrial fermentations and human disease treatment). (usda.gov)
Recombinant1
  • Experiment outside containment (clinical trial) for the study of the safety of a sucutaneous administration of a recombinant canarypoxvirus expressing FELV genes. (biosafety.be)
Organism2
  • Lactococcus lactis is a gram-positive bacterium used extensively in the production of buttermilk and cheese, but has also become famous as the first genetically modified organism to be used alive for the treatment of human disease. (wikipedia.org)
  • in this organism they map at 46.70 min of the gene map, equivalent to about 2180-2186 kbp. (rhea-db.org)
Strain4
  • Therefore, in the present study, a new human skin-derived Lactococcus paracasei strain, HS-05, was isolated, and its morphological characteristics were investigated. (springeropen.com)
  • Biologically contained strain of Lactococcus lactis, genetically modified to secrete human Trefoil Factor 1 (hTFF1). (biosafety.be)
  • Two adjacent genes encoding an Abi system were identified on the prophage sequence of a beer-spoiling Lb. brevis strain. (ucc.ie)
  • The leuB gene, crucial in the leucine biosynthetic pathway, was cloned onto plasmid p582 and the new construct transformed into an E. coli Keio (Delta leuB) knockout strain. (uni-bielefeld.de)
RRNA gene2
  • Further analyses of gut microbiota using next-generation sequencing (NGS) of the 16S rRNA gene showed that the pattern of gut microbial composition was clearly altered following 8 weeks of TWK10 administration. (frontiersin.org)
  • PCR-RFLP method, based on the amplification and restriction digestion of 16S rRNA gene, was found to be useful for the identification. (iyte.edu.tr)
Enterococcus1
Isolates1
  • Dairy isolates are suggested to have evolved from plant isolates through a process in which genes without benefit in the rich milk were lost or downregulated. (wikipedia.org)
Bacteriocin1
Lactic acid1
  • The capability to produce lactic acid is one of the reasons why L. lactis is one of the most important microorganisms in the dairy industry. (wikipedia.org)
Plasmid1
  • Moreover, novel molecular players were identified revealing the importance of chromosomal genes for general stress response (pH tolerance), and the relevance of plasmid-encoded genes for beer-specific stress response (such as tolerance to hop compounds). (ucc.ie)
Bacterium1
Streptococcus1
Protein6
  • Many different expression systems of L. lactis have been developed and used for heterologous protein expression. (wikipedia.org)
  • Pontes DS, de Azevedo MS, Chatel JM, Langella P, Azevedo V, Miyoshi A (2011) Lactococcus lactis as a live vector: heterologous protein production and DNA delivery systems. (springer.com)
  • Lactococcus lactisMG1363 expresses a protein, YfiA(Ll), which associates with ribosomes in the stationary phase of growth and is responsible for dimerization of ribosomes. (rug.nl)
  • Two variants were developed, oriented to nucleocapsid phosphoprotein gene (N) and to spike protein gene (S), respectively. (bvsalud.org)
  • First, a model viral system was constructed, containing a fragment of the SARS-CoV-2 gene encoding for the Spike protein. (bvsalud.org)
  • Since such systems have rarely been applied in continuous culture it seemed to be attractive to study the interplay between growth-phase regulated promoters controlling release protein genes and the productivity of a chemostat process. (uni-bielefeld.de)
Assays1
  • The performance of conventional qRT-PCR assays oriented to these genes with two novel OSN-qRT-PCR assays were firstly optimized using wastewater artificially contaminated with two encapsidated RNA mimic systems harboring a portion either N or S gene (ENRM and ESRM, respectively). (bvsalud.org)
Coli3
  • We show that full-length YfiA(Ll) is necessary and sufficient for ribosome dimerization in L.lactis but also functions heterologously in vitro with E.coli ribosomes. (rug.nl)
  • It is concluded that the dimerization and inactivation of ribosomes in L.lactis and E.coli differ in several cellular and molecular aspects. (rug.nl)
  • In addition, two-dimensional maps of dimeric ribosomes from L.lactis obtained by single particle electron microscopy show a marked structural difference in monomer association in comparison to the ribosome dimers in E.coli. (rug.nl)
Putative1
  • L. agilis BKN88 contains five putative chemoreceptor genes ( mcp1 - mcp5 ). (nature.com)
Dairy products1
Operons1
  • Expression of truncated bio transport operons, each with one gene deleted, resulted in stable BioMN complexes but revealed only low amounts of BioMY and BioNY aggregates in the absence of the respective third partner. (tcdb.org)
Motility1
  • It is suggested that the L. lactis supernatant mainly affects Salmonella motility through disruption of flagellar rotation rather than through irreversible damage to morphology and physiology. (wikipedia.org)
Structural2
  • Is the Subject Area "Structural genes" applicable to this article? (plos.org)
  • Structural features of multiple nifH-like sequences and very biased codon usage in nitrogenase genes of Clostridium pasteurianum. (microbiologyresearch.org)
Functional1
  • In order to exploit the functional properties of the ysbC gene it was investigated if an orotate analogue fluoro-orotate could be transported by YsbC. (biomedcentral.com)
Sequence2
Milk1
  • Consistent with the results from comparative genomics (see references above), this resulted in L. lactis losing or downregulating genes that are dispensable in milk and the upregulation of peptide transport. (wikipedia.org)
Mainly isolated1
  • L. lactis is mainly isolated from either the dairy environment, or plant material. (wikipedia.org)
Characteristics2
  • By contrast, ectopic expression of FnBPA or FnBPB on the surface of L. lactis conferred fibronectin binding characteristics similar to those of S. aureus. (duke.edu)
  • The Giardia lamblia vsp gene repertoire: characteristics, genomic organization, and evolution. (mbl.edu)
Motile1
  • L. lactis does not produce spores (nonsporulating) and are not motile (nonmotile). (wikipedia.org)
Promoter1
  • The genes are transcribed from a promoter located upstream of gatY. (rhea-db.org)
Phylogenetic1
  • Phylogenetic affiliations of mesopelagic acantharia and acantharian-like environmental 18S rRNA genes off the southern California coast. (mbl.edu)
Experiments1
Growth2
  • L. lactis cells are cocci that group in pairs and short chains, and, depending on growth conditions, appear ovoid with a typical length of 0.5 - 1.5 µm. (wikipedia.org)
  • Deletion of the yfiA gene has no effect on the growth rate but diminishes the survival of L.lactis under energy-starving conditions. (rug.nl)
Synthesis1
  • Many bioY genes are located at loci encoding biotin biosynthesis or are unlinked to either biotin synthesis or other biotin transport genes. (tcdb.org)
Salmonella1
  • Lactose fermentation by L. lactis produces acetate that reduces the intracellular pH of Salmonella, which in turn slows the rotation of their flagella. (wikipedia.org)
Comparisons1
  • Lactococcus lactis Diversity Revealed by Targeted Amplicon Sequencing of purR Gene, Metabolic Comparisons and Antimicrobial Properties in an Undefined Mixed Starter Culture Used for Soft-Cheese Manufacture. (bionotatki.com)
Transport1
  • were transformed with lactococcal genes for an extracellular serine proteinase or an oligopeptide transport system. (usu.edu)
Transporter1
  • This analysis allowed the identification of a chromosomal gene encoding a presumed manganese transporter involved in low pH tolerance in Lb. brevis. (ucc.ie)