ViralProteinsRetroviral vectorsGenomeLentiviral vectorTransgenicAdenoviral vectorsTransgene expressionPromoterGenomicVirusesCDNAVitroGeneticTransductionTherapyEukaryoticTumorCRISPRHemophiliaMammalian brainRegulationCellsExpress genesRetrovirusPromotersTransientReporterInductionVivoOverexpressionRAAVTherapeuticDifferential gene expressionFunctionalCLINICALHomologousSynthesisTumourGenomesTargetingPatternsProtein expressionMicroRNA expressionSpeciesPlasmidsQuantitativeMethodsDifferentially
Viral15
- The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods). (wikipedia.org)
- Thus, we will develop novel targeted viral vectors for applications to cancer gene therapy. (europa.eu)
- Targeting viral vector particles to predetermined cell types will be obtained through genetic engineering of retroviral envelopes or adenoviral capsid proteins, by using single chain antibody fragments or tumour cell receptor ligands. (europa.eu)
- This proposal brings together European leaders with expertise covering from the development of novel viral vectors to the implementation of clinical trials of gene therapy. (europa.eu)
- Our general objective is the development of new generations of targeted viral vectors for the treatment of liver and brain cancer by directing viral entry and by restricting transgene expression. (europa.eu)
- Once new generation targeted viral vectors are generated, they will be sent to the appropriate partners for the in vitro testing of the efficiency of the novel vectors in various cell lines. (europa.eu)
- To this end, all new vectors will express the same identical therapeutic transgene, which will allow comparisons to be made across all novel viral vectors produced. (europa.eu)
- The workprogram has been divided in 6 workpackages each corresponding to a targeted vector (retro-, lenti- or adeno-viral) tested in a specific tumour model (liver or brain). (europa.eu)
- These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. (cshlpress.com)
- Crucially, some viral vectors (i.e., viruses specifically used to deliver genetic material into cells) have the potential to circumvent the blood-brain- (BBB) and blood-spinal cord barriers (BSCB) when intravenously injected. (frontiersin.org)
- Viral vectors such as those based on adeno-associated viruses (AAVs) and lentiviruses (LVs) are used as vehicles to deliver functional genes to affected hepatocytes. (bvsalud.org)
- In this study, we showed that a one-hour treatment with topoisomerase inhibitors (camptothecin and etoposide) prior to viral transduction is enough to increase AAV and LV reporter expression in non-dividing hepatic cells in culture. (bvsalud.org)
- As the clinical experience in adeno-associated viral (AAV) vector-based gene therapies is expanding, the necessity to better understand and control the host immune responses is also increasing. (bvsalud.org)
- The adeno-associated viral vector (AAV) provides a safe and efficient gene therapy platform with several approved products that have marked therapeutic impact for patients. (bvsalud.org)
- Strategies for gene therapy and production of medicines via genetically-modified organisms (expression vectors and viral vectors). (lu.se)
Proteins11
- pSELECT-Tag is a family of expression plasmids designed to generate tagged proteins in order to facilitate their detection and/or purification. (invivogen.com)
- The differentially expressed genes included those functioning in some important immune reaction and iron metabolism pathways, such as pattern recognition receptors, regulators of the JNK pathway, components of the phagosome pathway, regulators of the melanization response, activators of complement reactions, insulin signaling cascade members, oxidative stress and detoxification proteins. (biomedcentral.com)
- Soybean gene functions cannot be easily interrogated through transgenic disruption (knock-out) of genes-of-interest, or transgenic overexpression of proteins-of-interest, because soybean transformation is time-consuming and technically challenging. (researchsquare.com)
- Meanwhile, FZ supported systemic expression of fluorescent proteins mNeonGreen and mCherry in N. benthamiana and soybean. (researchsquare.com)
- The expression levels of TRAF proteins were normalized to that of actin. (biomedcentral.com)
- ii) gutless adenoviral vectors, by genetic modifications of the fibre and hexon proteins of the adenoviral capsids, as well as through the use of combined cell type specific and inducible promoters. (europa.eu)
- 19. Mara C D,Huang T,Irish V F. The Arabidopsis floral homeotic proteins APETALA3 and PISTILLATA negatively regulate the BANQUO genes implicated in light signaling[J].Plant Cell,2010,22(3):690-702. (nefu.edu.cn)
- Eukaryotic expression vectors containing genes encoding plant proteins for killing of cancer cells. (weeksmd.com)
- consequently, genes encoding some of these proteins are being used to design constructs for the inhibition of multiplying cancer cells. (weeksmd.com)
- Our selection of CMV vectors provides options for transient or stable expression of 3xFLAG ® fusion proteins. (sigmaaldrich.com)
- Vectors containing the preprotrypsin leader (PPT) sequence direct secretion of FLAG fusion proteins into the culture medium for purification using ANTI-FLAG antibodies, resins, and plates. (sigmaaldrich.com)
Retroviral vectors1
- Gene therapy trials using retroviral vectors to treat X-linked severe combined immunodeficiency (X-SCID) represent the most successful application of gene therapy to date. (wikipedia.org)
Genome12
- To understand the differential gene expression in Anopheles following infection with drug-resistant Plasmodium , we conducted a genome-wide transcriptomic profiling analysis of Anopheles stephensi following feeding on mice with drug-resistant or drug-sensitive P. yoelii , observed changes in gene expression profiles and identified transcripts affected by the drug-resistant parasite. (biomedcentral.com)
- We describe the development of a new vector based on cowpea severe mosaic virus (CPSMV), a plus-strand RNA virus with its genome divided into two RNA segments, RNA1 and RNA2. (researchsquare.com)
- In this study, we first identified all the CAMTA homologous genes in the whole genome of maize. (frontiersin.org)
- CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications, not only for single gene targeting, but also for multiple targeted mutagenesis. (nature.com)
- Our system provides an efficient targeting strategy for multiplex genome/epigenome editing, simultaneous activation/repression of multiple genes and beyond. (nature.com)
- Genome editing using clustered regularly-interspaced short palindromic repeats (CRISPR)/Cas9 is an easy and efficient strategy for the generation of gene-modified cells and organisms 1 , 2 . (nature.com)
- In Cas9 nuclease-mediated genome editing, we constructed an all-in-one vector expressing Cas9 nuclease and seven gRNAs and targeted seven genomic loci. (nature.com)
- By PCR, the AtPI gene was integrated into the tobacco genome. (nefu.edu.cn)
- Moreover, genome instability was attributed to impaired autophagy and several autophagy genes with tumor suppressor functions (e.g. (hindawi.com)
- More than half (23 of 39) of the articles represent epidemiologic studies including candidate gene and genome-wide association studies (GWAS) examining genetic effect modification on disease risk. (cdc.gov)
- Critical to sub-classify SRBCTs, Khan et al .6 used to measure genome-wide gene expres- .com decisions in cancer therapeutics are to microarrays to monitor the expression sion for a variety of biological samples. (lu.se)
- Finally, permanent modification of the host genome associated with rAAV genome integration and gene editing raises concerns about the risk of oncogenicity that require careful evaluation. (bvsalud.org)
Lentiviral vector1
- This Lenti vector is a third generation of lentiviral vector. (origene.com)
Transgenic5
- Transgenic cell line overexpressing the eEF1sigma gene were generated by transfecting HeLa cells with the expression vector pcDNA 3.1 containing human eEF1sigma cDNA. (cdc.gov)
- Expression of eEF1sigma in the transgenic cell line was determined by real-time PCR and Western blot analysis. (cdc.gov)
- The transgenic cell line exhibited enhanced cell proliferation, overexpression of eEF1sigma, eIF4E and eEF2 genes compared with the control cell line. (cdc.gov)
- Large-cage assessment of a transgenic sex-ratio distortion strain on populations of an African malaria vector. (cdc.gov)
- Our technical expertise include lentiviral vectors, transgenic mice and stem cells cultures. (lu.se)
Adenoviral vectors2
Transgene expression2
- For successful transgene expression, viruses administered into muscle must undergo a series of processes, including host cell interaction and internalization, intracellular sorting, long-range retrograde axonal transport, endosomal liberation, and nuclear import. (frontiersin.org)
- Immunogenicity of AAV vectors in humans has been linked to several limitations of the platform, including lack of efficacy due to antibody-mediated neutralization, tissue inflammation, loss of transgene expression, and in some cases, complement activation and acute toxicities. (bvsalud.org)
Promoter10
- In this protocol chapter we describe our work using episomal vectors where expression of a cDNA is controlled by a 10 kB piece of genomic DNA encompassing the promoter of the low density lipoprotein receptor. (ox.ac.uk)
- A liver specific promoter with regulatory regions minimized in size is provided, that can be used to improve expression levels - a key for making gene therapy work in humans. (stjude.org)
- This promoter has been licensed for use in gene therapy vectors to treat hemophilia B. We are currently seeking licensing opportunities for all other uses, with a specific interested in the use of this promoter in gene therapy vectors to drive liver-specific expression of desired coding sequences beyond the current use with factor IX. (stjude.org)
- We then surveyed the distribution of stress-related cis -regulatory elements in the −1.5 kb promoter regions of ZmCAMTA genes. (frontiersin.org)
- Notably, a large number of stress-related elements present in the promoter regions of some ZmCAMTA genes, indicating a genetic basis of stress expression regulation of these genes. (frontiersin.org)
- CAMTAs participate in gene expression regulation by binding to the cis -elements in the promoter regions of numerous target genes. (frontiersin.org)
- Transcriptional targeting of transgenes expression to tumour cells will be obtained using tumour cell type specific promoter. (europa.eu)
- Our mammalian expression vectors contain the strong CMV promoter for high-level constitutive expression in mammalian cells. (sigmaaldrich.com)
- The strong human cytomegalovirus (CMV) promoter regulatory region drives constitutive protein expression levels as high as 1 mG/L in COS cells. (sigmaaldrich.com)
- Methods for analysis of gene expression: microarray, hybridisation, promoter analyses. (lu.se)
Genomic3
- Extrachromosomal gene expression vectors that contain native genomic gene expression elements have numerous advantages over traditional integrating mini-gene vectors. (ox.ac.uk)
- We explain methods to sub-clone large genomic inserts into gene expression vectors. (ox.ac.uk)
- Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning[J].Proc Natl Acad Sci USA,1999,96(11):6535-6540. (nefu.edu.cn)
Viruses4
- In this paper we describe how recombinant vaccinia viruses are constructed and illustrate the potential of this vector system for expression of genes from parasitic pathogens. (ox.ac.uk)
- Administration of gene therapy viruses into skeletal muscle, where distal terminals of motor and sensory neurons reside, has been shown to result in extensive transduction of cells within the spinal cord, brainstem, and sensory ganglia. (frontiersin.org)
- In this review article, we outline key characteristics of major gene therapy viruses-adenovirus, adeno-associated virus (AAV), and lentivirus-and summarize the mechanisms regulating important steps in the virus journey from binding at peripheral nerve terminals to nuclear delivery. (frontiersin.org)
- Gene therapy viruses are non-replicating, but still hijack host cell machinery to express transgenes of interest in the nucleus. (frontiersin.org)
CDNA5
- This vector, designated FZ, incorporates a cloning site in the RNA2 cDNA, permitting insertion of nonviral sequences. (researchsquare.com)
- Methods: In the present study, we constructed bipartite plasmid vectors to co-express a vascular endothelial growth factor (VEGF) cDNA and short hairpin (sh)RNA targeting inducible NO synthase (iNOS) gene. (nebraska.edu)
- Conclusions: In the present study, we have successfully constructed bipartite vectors co-expressing a VEGF cDNA and a shRNA against the iNOS gene. (nebraska.edu)
- Li, F & Mahato, RI 2009, ' Bipartite vectors for co-expression of a growth factor cDNA and short hairpin RNA against an apoptotic gene ', Journal of Gene Medicine , vol. 11, no. 9, pp. 764-771. (nebraska.edu)
- In order to explore the function of PI gene in the development of petals and stamens, the PI gene in Arabidopsis thaliana was used as the research object, and the AtPI gene was amplified from cDNA by PCR, and the plant expression vector(pROKⅡ- AtPI ) was constructed and transformed into tobacco. (nefu.edu.cn)
Vitro2
- The characteristics, efficiency, and toxicity of targeted vectors will first be assessed in vitro on relevant cell lines and primary cell cultures, then in vivo using animal models of brain and liver cancer. (europa.eu)
- The in vitro testing will allow selection of the most efficient vectors which will then be chosen to be further studied. (europa.eu)
Genetic10
- Now, using genetic engineering, infectious vaccinia virus recombinants are being constructed which express genes from other pathogens. (ox.ac.uk)
- citation needed] Now that the genetic material of the virus has been inserted, it can be said that the host cell has been modified to contain new genes. (wikipedia.org)
- If genetic material happens to be inserted in the middle of one of the original genes of the host cell, this gene will be disrupted (insertional mutagenesis). (wikipedia.org)
- Construction of Plant Expression Vector and Genetic Transformation Analysis of Arabidopsis thaliana AtPI Gene in Nicotiana tabacum [J]. Bulletin of Botanical Research, 2016, 36(3): 388-394. (nefu.edu.cn)
- Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. (cshlpress.com)
- Virus-mediated gene therapy has the potential to deliver exogenous genetic material into specific cell types to promote survival and counteract disease. (frontiersin.org)
- Genetic changes in microbes, animal vectors, and humans were included. (cdc.gov)
- Gene therapy is poised to revolutionize modern medicine, with seemingly unlimited potential for treating and curing genetic disorders. (bvsalud.org)
- Single-Gene Defects Genetic disorders determined by a single gene (Mendelian disorders) are easiest to analyze and the most well understood. (msdmanuals.com)
- Gene technology: basic and applied molecular genetic methods. (lu.se)
Transduction2
- We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
- Topoisomerase inhibitors increased both integration-competent (ICLV) and integration-deficient (IDLV) LV-derived expression, with a much stronger increase in the IDLV transduction system. (bvsalud.org)
Therapy15
- and we believe it can be further used to express any gene of interest in a size-constrained environment, such as in a self-complementary gene therapy vector system. (stjude.org)
- Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. (wikipedia.org)
- Similar trials were restricted or halted in the US when leukemia was reported in patients treated in the French X-SCID gene therapy trial. (wikipedia.org)
- Gene therapy trials to treat SCID due to deficiency of the Adenosine Deaminase (ADA) enzyme (one form of SCID) continue with relative success in the US, Britain, Ireland, Italy and Japan. (wikipedia.org)
- Ongoing anti-cancer gene therapy clinical trials have highlighted the need for more efficient and safer vectors. (europa.eu)
- We thus aim to obtain well characterized, safe, and efficient gene therapy vectors, as well as pre-clinical data supporting the further testing of such vectors in clinical trials. (europa.eu)
- Gene therapy has attracted attention for its potential to specifically and efficiently target cancer cells with minimal toxicity to normal cells. (weeksmd.com)
- This route is minimally invasive and therefore clinically relevant for gene therapy targeting to peripheral nerve soma. (frontiersin.org)
- With thousands of clinical trials to date, gene therapy is a flourishing strategy with great promise for the treatment of diseases impacting the nervous system. (frontiersin.org)
- Gene therapy is a promising strategy to treat and cure most inherited metabolic liver disorders. (bvsalud.org)
- For otherwise incurable indications, including most inherited metabolic liver disorders, gene therapy provides a realistic therapeutic option. (bvsalud.org)
- We summarize the main progress, challenges and pathways forward for gene therapy for liver diseases. (bvsalud.org)
- Additionally, for patients with GSD type I, the future may bring adeno-associated virus vector - mediated gene experimental therapy, which may result in curative therapy, as is possible in patients with GSD type II. (medscape.com)
- Gene therapy is an encouraging mode of treatment but is not yet available. (medscape.com)
- [ 21 ] reported using a GSD type II mouse model, a new mode of gene therapy using muscle as a secretary organ, and an adenovirus vector encoding AdGAA. (medscape.com)
Eukaryotic2
- However, ST themselves might be immunosuppressive, and the question has arisen as to whether this impedes vaccination efficacy even if ST are transformed with a eukaryotic expression vector such that "tumor antigen" will be transcribed by the host. (tau.ac.il)
- Mice were vaccinated with SL7207 or SL7207ΔyejE transformed with a eukaryotic expression vector carrying the lacZ or the gp100 gene and later received lacZ-transfected RENCA or YC8 or gp100-expressing B16F1 tumor cells. (tau.ac.il)
Tumor2
- The authors used artificial neural croarrays is to study changes in gene ex- .nature organ the tumor arose from, how big it networks (ANNs) to identify gene-ex- pression that accompany changes in is, and what it looks like after it has pression signatures associated with spe- cell physiology, such as during develop- been imbedded in wax and colored cific subtypes of tumors. (lu.se)
- Red not have the tumor-specific ex- with these dangerous drugs, rhab- represents relatively high expression level, whereas green pression pattern. (lu.se)
CRISPR5
- To make the most of the multiplexity of CRISPR/Cas9, we established a system for constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette. (nature.com)
- We further demonstrated successful examples of multiple targeting including chromosomal deletions in human cells using the all-in-one CRISPR/Cas9 vectors constructed with our novel system. (nature.com)
- Here, we report an easy and efficient construction system for all-in-one CRISPR/Cas9 vectors expressing Cas9 protein and up to seven gRNAs. (nature.com)
- In addition, the plasmids for the second-step reaction retained the structure of a CRISPR/Cas9 expression vector harboring single gRNA and Cas9 expression cassettes and can therefore be used separately for single gene targeting. (nature.com)
- To develop vector-based strategies of CRISPR/Cas9-mediated gene editing in the brain for experimental and therapeutic purposes. (lu.se)
Hemophilia2
- Hemophilia is an inherited disorder that results from mutations, deletions, or inversions affecting the factor VIII or factor IX gene. (msdmanuals.com)
- Because these genes are located on the X chromosome, hemophilia affects males almost exclusively. (msdmanuals.com)
Mammalian brain2
- Efficiency and stability of recombinant adeno-associated virus (rAAV)-mediated gene expression within the mammalian brain are determined by several factors. (nih.gov)
- This review summarizes the characteristics of various transcriptional and posttranscriptional regulatory elements, and highlights their influence on the expression performance of rAAV vectors in the mammalian brain. (nih.gov)
Regulation7
- Understanding gene function and regulation requires rigorous testing in live cells and organisms. (cshlpress.com)
- Regulation Vector Clipart Royalty Free. (canstockphoto.com)
- 35,566 Regulation clip art vector EPS illustrations and images available to search from thousands of stock illustrators. (canstockphoto.com)
- To understand the role of the epigenetic co-repressor protein TRIM28 in the control of transposable elements (TEs) and how TEs control gene regulation in the developing and adult brain. (lu.se)
- Molecular mechanisms for regulation of gene expression at different levels: remodeling of chromatin, initiation of transcription, nuclear transport and signalling, and RNA interference. (lu.se)
- Principles for regulation of gene expression through intercellular signalling. (lu.se)
- Gene regulation in developmental biology and the cell cycle. (lu.se)
Cells10
- These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)
- Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. (cshlpress.com)
- For example, cerebellar granule cells express six subunit genes abundantly (α 1 , α 6 , β 2 , β 3 , γ 2 , and δ), and so they probably have several distinct GABA A receptor subtypes of unknown subunit stoichiometry. (jneurosci.org)
- The underlying mechanism responsible for the effects of lunasin on NK cells is likely due to epigenetic modulation on target gene loci. (weeksmd.com)
- Numerous vectors have been engineered for the sole purpose of killing cancer cells, and some have successfully suppressed malignant tumours. (weeksmd.com)
- Ideal for cases of low-level expression in mammalian cells. (sigmaaldrich.com)
- The gene product, Wiskott-Aldrich Syndrome Protein (WASp) is a 502 amino acid protein expressed within the cytoplasm of non-erythroid hematopoietic cells. (medscape.com)
- The gene resides on Xp11.22-23, and its expression is limited to cells of non-erythroid hematopoietic lineage. (medscape.com)
- When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)
- The thesis work would focus on identification of genes that are essential for the optimal functioning of the ubiquitin-proteasomal system in leukemic cells. (lu.se)
Express genes1
Retrovirus2
- Factors involved in production of helper virus-free retrovirus vectors. (wikidata.org)
- Sometimes the genes of the retrovirus do not express their information immediately. (wikipedia.org)
Promoters3
- Then, the effect of different promoters [such as H1, U6 and cytomegalovirus (CMV)] and micro RNA backbones on gene silencing was determined. (nebraska.edu)
- Results: No statistical difference in iNOS gene silencing was observed for the shRNA with H1, U6 and CMV promoters. (nebraska.edu)
- Results: Data addressing the function of vectors harbouring genes specifically encoding ricin, saporin, lunasin , linamarase, and tomato thymidine kinase 1 under the control of different promoters are summarised here. (weeksmd.com)
Transient1
- An attractive alternative is to administer transient gene silencing or overexpression with a plant virus-based vector. (researchsquare.com)
Reporter3
- All vectors will express a reporter/suicide gene, and they will be tested using in vivo models of liver and brain tumours. (europa.eu)
- A common suicide/reporter gene will first be used for all vectors, and then additional therapeutic genes will be tested. (europa.eu)
- While nine spCas9 gene editing sites surround the Arc start codon, the accuracy of the editing was highly sequence-dependent, with only a single target resulting in an in-frame reporter integration. (lu.se)
Induction1
Vivo3
- Furthermore, the choice of appropriate regulatory elements in rAAV vector design is of fundamental importance to achieve high-level sustained in vivo transcription and translation. (nih.gov)
- Delivery of gene expression vectors in vivo is also described using hydrodynamic tail vein injection, a high pressure, high volume tail vein injection used for liver-directed gene transfer. (ox.ac.uk)
- During Phase-II production of the most efficient vectors will be scaled up, and distributed to all appropriate partners for the in vivo testing and comparison in available models. (europa.eu)
Overexpression1
- However, existing virus-induced gene silencing (VIGS) and/or overexpression vectors suitable for soybean have various drawbacks that hinder their widespread adoption. (researchsquare.com)
RAAV2
- These include the dose of infectious particles, the purity of the vector stock, the serotype of rAAV, the route of administration, and the intrinsic properties, most notably the rAAV receptor density, of the targeted area. (nih.gov)
- In this Review, we discuss gene supplementation and gene editing involving the use of recombinant adeno-associated virus (rAAV) vectors for the treatment of inherited liver diseases, including updates on several ongoing clinical trials that are producing promising results. (bvsalud.org)
Therapeutic2
- This leads to Phase-III of this research programme in which the most powerful targeted vectors will be chosen to be developed further, by inserting novel powerful therapeutic genes into them. (europa.eu)
- Additionally, we describe how neuropathology can negatively influence these pathways, and conclude by discussing opportunities to optimize the intramuscular administration route to maximize gene delivery and thus therapeutic potential. (frontiersin.org)
Differential gene expression1
- RNA sequencing is a powerful method to study differential gene expression because it can detect a whole gene expression across particular tissues 1 . (nature.com)
Functional3
- The new CPSMV-derived FZ vector provides a convenient and versatile soybean functional genomics tool that is expected to accelerate the characterization of soybean genes controlling crucial productivity traits. (researchsquare.com)
- Virus-induced gene silencing (VIGS) is an alternative functional genomics tool for soybean because it does not involve the generation of inheritable changes. (researchsquare.com)
- The flower is the main reproductive organs of the plants, plays an extremely important role in the process of breeding and the PISTILLATA(PI) gene, and as a member of the B functional gene, plays an important role in floral organ development. (nefu.edu.cn)
CLINICAL2
- Depending on the mutations within the WASp gene product, there is wide variability of clinical disease. (medscape.com)
- Nevertheless, significant knowledge gaps remain in our understanding of the mechanisms of immune responses to AAV gene therapies, further hampered by the failure of preclinical animal models to recapitulate clinical findings. (bvsalud.org)
Homologous1
- Exon 8 of the mouse α 6 subunit gene was disrupted by homologous recombination. (jneurosci.org)
Synthesis1
- Finally, FZ-mediated expression of the Arabidopsis transcription factor MYB75 caused N. benthamiana to bear brown leaves and purple, twisted flowers, indicating that MYB75 retained the function of activating anthocyanin synthesis pathways in a different plant. (researchsquare.com)
Tumour1
- This second round of selection will lead to our choice of the most powerful vectors for each tumour models. (europa.eu)
Genomes1
Targeting1
- Low expression of the autophagy initiator ULK1 in AML can partially be attributed to high expression of the ULK1-targeting microRNA-106a. (hindawi.com)
Patterns6
- Our results highlight the need for a better understanding of selective pressures from these differentially expressed genes of the drug-resistant Plasmodium in the mosquito and the different transmission patterns of drug-resistant Plasmodium by Anopheles . (biomedcentral.com)
- Different gene expression patterns, shown by tools such as quantitative PCR, microarrays, and high-throughput RNA sequencing, reveal how organisms respond to different environments. (nature.com)
- The results showed that nine ZmCAMTA genes showed highly diversified gene structures and tissue-specific expression patterns. (frontiersin.org)
- 0 pression patterns of 96 transcripts and prognosis based on a limited representing 80 known genes and 20 13 numbers of markers1-5. (lu.se)
- Gene-expression patterns used to differentiate four type cause heart damage, and markable finding that genes cur- types of SRBCT. (lu.se)
- Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
Protein expression2
- In general, WAS gene mutations that cause absent protein expression result in classic WAS. (medscape.com)
- Reduced WASp protein expression results in X-linked thrombocytopenia. (medscape.com)
MicroRNA expression1
- Many other molecular paramaters such as global or alelle-specific DNA methylation, gene expression levels, and microRNA expression levels can also be considered. (columbia.edu)
Species2
- Here, we investigated, in females of both D. melanogaster and D. nigrosparsa , the gene expression between animals uninfected and infected with w Mel, using RNA sequencing to see if the two Drosophila species respond to the infection in the same or different ways. (nature.com)
- Significant changes shared by the fly species belong to the expression of genes involved in processes such as oxidation-reduction process, iron-ion binding, and voltage-gated potassium-channel activity. (nature.com)
Plasmids1
- In this study, we constructed plasmids to create all-in-one vectors expressing two to seven gRNAs with Cas9 nuclease or nickase ( Figure 1 ). (nature.com)
Quantitative1
- Quantitative real-time PCR was used to test the expression of ZmCAMTA genes under several abiotic stresses (drought, salt, and cold), various stress-related hormones [abscisic acid, auxin, salicylic acid (SA), and jasmonic acid] and biotic stress [rice black-streaked dwarf virus (RBSDV) infection]. (frontiersin.org)
Methods3
- T. Twellmann, T.W. Nattkemper, W. Schubert, H. Ritter, Cell detection in micrographs of tissue sections using support vector machines, in: Proceedings of the ICANN: workshop on Kernel & Subspace Methods for Computer Vision, Vienna, Austria, 2001, pp. 79–88. (crossref.org)
- We also illustrate various methods employed to ascertain whether expression from these vectors is robust and physiologically relevant by investigating their sensitivity to changes in cellular milieu. (ox.ac.uk)
- Methods for gene identification and analysis of gene structure: cloning, PCR, restriction mapping, in situ hybridisation, DNA sequencing. (lu.se)
Differentially2
- Our study shows that drug-resistant P. yoelii NSM has an impact on the transcript abundance levels of An.stephensi mostly at 13 days after blood meal during sporozoite migration through the hemolymph and that most differentially expressed genes were downregulated. (biomedcentral.com)
- Recessive loci Pps-1 and OM differentially regulate PISTILLATA-1 and APETALA3-1 expression for sepal and petal development in Papaver somniferum [J].PLoS One,2014,9(6):e101272. (nefu.edu.cn)