• Constitutive expression of Hsp70 during development may afford these embryos protection from environmental stresses during development more quickly than relying on the induction of a classic heat shock response. (bioone.org)
  • Although the heat-acclimated and unacclimated larvae upregulated expression of heat shock protein genes under heat stress including HSP70 , HSP27 and CRYAB , their biosynthesis, metabolism and detoxification-related genes expressed differentially. (biomedcentral.com)
  • The co-chaperone Hep1 is required to prevent the aggregation of mitochondrial Hsp70 proteins. (cipsm.de)
  • Pertinent to AD pathophysiology, heat shock protein 90 (HSP90)/co-chaperone complex folds tau or hyperphosphorylated tau, whereas heat shock protein 70-carboxyl-terminus of HSP70 Interacting protein (HSP70-CHIP) complex mediates degradation 13 , 14 . (nature.com)
  • Exosomal markers include microRNAs like miR-21 and miR-141, plus various proteins that belong in functional groups such as tetraspanins (CD9, CD63 and CD81), heat shock proteins (Hsp70, Hsp73 and Hsp90) and membrane transporters (GTPases) ( 4 ). (spandidos-publications.com)
  • Exposure to 41 °C induced 1160 differentially expressed genes (DEGs) between the heat-acclimated and unacclimated larvae. (biomedcentral.com)
  • The intersection between the differentially expressed genes (DEGs) and the HNK-targets was determined using a Venn diagram, and the results were analyzed using a protein-protein interaction network, hub gene selection, gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses, genetic alteration analysis, survival rate, and immune cell infiltration levels. (hindawi.com)
  • a Heatmap of mRNA-Seq Poziotinib analysis of differentially expressed genes (2-fold change and FDR? (neuroart2006.com)
  • Heat shock proteins (HSPs) are highly conserved and immunogenic proteins that are shared among diverse groups of mammals and microbial agents. (eg.net)
  • Molecular chaperones are known to play a role in stabilizing protein structure and function during events of cellular stress. (bioone.org)
  • HSP90) and induction of chaperones promoting tau degradation (e.g. (nature.com)
  • The mitochondria have their own chaperones and proteolytic enzymes that remove damaged or unfolded proteins [ 18 - 20 ]. (hindawi.com)
  • Relative levels of heat shock protein (Hsp)70 were measured in developing and diapausing embryos of A. limnaeus using quantitative Western blots. (bioone.org)
  • It provides a very informative, albeit preliminary, glimpse into the expression of genes encoding functionally relevant proteins as potential targets for chemotherapy, and evidence for the presence of a relict plastid. (biomedcentral.com)
  • Pathway inference tools were used to interpret these proteins in terms of functionally enriched pathways in the tumor cell lines. (biomedcentral.com)
  • Interestingly, functionally redundant SNIPER1 and SNIPER2 can control the protein levels of diverse sNLRs and the interactions between SNIPER1 and sNLRs appear to be through the common nucleotide-binding (NB) domains of sNLRs. (ubc.ca)
  • Environmental injury has been associated with endoplasmic reticulum (ER) stress, a response characterized by activation of the unfolded protein response, proteasomal degradation of proteins, and induction of HSPA5, also known as GRP78 or BiP. (bioone.org)
  • This gene encodes a protein that binds to the tumor suppressor folliculin and to AMP-activated protein kinase (AMPK), and may play a role cellular metabolism and nutrient sensing by regulating the AMPK-mechanistic target of rapamycin signaling pathway. (nih.gov)
  • TRAP1, a crucial member of the mitochondrial heat shock protein 90 family, is active in a variety of biological functions in various tumor types. (scientificarchives.com)
  • tumor suppressor genes. (upstate.edu)
  • OVs armed with GM-CSF (such as T-VEC and Pexa-Vec) or other immunostimulatory genes, induce potent anti-tumor immunity in both animal models and human patients. (biomedcentral.com)
  • Acquiring one of the hallmarks of cancer is associated with activating/deactivating particular genes, called oncogenes/tumor suppressors. (biomedcentral.com)
  • High-throughput quantitative proteomic analysis has previously been used to identify differentially expressed proteins and pathways associated with breast tumor phenotypes [ 12 ]. (biomedcentral.com)
  • The encoded protein may also be involved in regulating the O6-methylguanine-induced apoptosis signaling pathway. (nih.gov)
  • Both the acclimated and unacclimated larvae adjusted the longevity regulating, protein processing in endoplasmic reticulum, antigen processing and presentation, MAPK and estrogen signaling pathway to responsed to heat stress. (biomedcentral.com)
  • However, relating gene expression with the specific role that the corresponding protein plays in a signaling pathway is complicated by post-transcriptional control of protein expression [ 8 ] and tight regulation of protein activity [ 9 ]. (biomedcentral.com)
  • The Arabidopsis thaliana and Nicotiana tabacum transgenic lines overexpressing the GmGlb1-1 gene showed reduced susceptibility to M. incognita. (bvsalud.org)
  • Here, using a combination of RNA-seq and proteomic LC-MS/MS analyses, we investigated the effect of anesthetic diethyl ether on gene expression and downstream consequences in plant Arabidopsis thaliana . (frontiersin.org)
  • The genome of Arabidopsis thaliana however, encodes for eight different isoforms. (cipsm.de)
  • HSP90 inhibition may also enhance synaptic protein expression via activation of heat shock factor 1 (HSF-1) transcriptional regulation 16 . (nature.com)
  • Endoplasmic reticulum stress (ERS) is a state induced by increased synthesis of intracellular proteins, accumulation of mal-folded proteins, changes in cytosolic calcium levels, or imbalances in endoplasmic reticulum (ER) redox homeostasis [ 8 ]. (biomedcentral.com)
  • These result in imbalances in the connectivity of neuronal circuitry, and also negatively impact the intracellular connectivity of neuronal proteins and protein pathways, contributing to cognitive decline associated with AD 6 . (nature.com)
  • Plant intracellular nucleotide binding and leucine-rich repeat proteins (NB-LRR, NLRs) function as immune receptors to detect microbial pathogens directly or indirectly. (ubc.ca)
  • Stabilization of MAPO1 by specific binding with folliculin and AMP-activated protein kinase in O⁶-methylguanine-induced apoptosis. (nih.gov)
  • We observed "protein ubiquitination" and "apoptosis signaling" pathways were both enriched in the two breast cancer models while "IGF signaling" and "cell motility" pathways were enriched in BT474 and "amino acid metabolism" were enriched in the SKBR3 cell line. (biomedcentral.com)
  • While "protein ubiquitination" and "apoptosis signaling" pathways were common to both the cell lines, the observed patterns of protein expression suggest that the evasion of apoptosis in each tumorigenic cell line occurs via different mechanisms. (biomedcentral.com)
  • The DEGs between heat-acclimated and unacclimated larvae after heat shock were enriched in the biosynthesis and metabolism pathways. (biomedcentral.com)
  • The objectives of this study were to quantify alterations in protein expression in two HER2+ cellular models of breast cancer and to infer differentially regulated signaling pathways in these models associated with the hallmarks of cancer. (biomedcentral.com)
  • The proteostasis network (PN) is an assembly of distinct dynamic molecular pathways that control the functionality of the proteome (proteome homeodynamics) during protein synthesis, folding, trafficking, and degradation. (hindawi.com)
  • Furthermore, the results proved that the Hsf1, an upstream transcription factor of Hsps, had a positive effect on alleviating the unfolded protein response and that overexpression of Hsf1 reprogramed the pattern of ATF1 gene transcript levels. (biomedcentral.com)
  • The most significantly upregulated process was the response to heat stress (mainly heat shock proteins, HSPs). (frontiersin.org)
  • The plants pre-treated with diethyl ether, and thus with induced HSPs, had increased tolerance of photosystem II to subsequent heat stress through the process known as cross-tolerance or priming. (frontiersin.org)
  • Heat shock protein 90kDa alpha (cytosolic), class A member 2, also known as HSP90AA2, is a human gene. (wikipedia.org)
  • Therefore, these collective data suggest that ectopic or nematode-induced overexpression, or enhanced expression of the GmGlb1-1 gene using CRISPR/dCas9 offers great potential for application in commercial soybean cultivars aiming to reduce plant susceptibility to M. incognita. (bvsalud.org)
  • nontargeting siRNA was used as handle.A significant lessen in complete MET protein expression was accomplished immediately after this knockdown.Most significantly, MET knockdown blocked ligand-induced activation of MET and resultant downstream signaling.MET knockdown didn't influence cell growth and proliferation but considerably inhibited constitutive and HGFinduced cell migration and invasion. (pafrinhibitors.com)
  • Temperature affects invertebrate hormone system, and the increased temperature induces expression of endocrine signaling genes of chironomids Chironomus riparius [ 14 ]. (biomedcentral.com)
  • In molecular biology and genetics, transactivation is an increased rate of gene expression triggered either by biological processes or by artificial means. (absoluteastronomy.com)
  • It is beneficial to improve the protein expression and product yield by controlling the promoter strength to fine regulate the transcription level of the target gene and maximize the transcription efficiency. (biomedcentral.com)
  • Differential expression analyses revealed reprogramming of gene expression under anesthesia: 6,168 genes were upregulated, 6,310 genes were downregulated, while 9,914 genes were not affected in comparison with control plants. (frontiersin.org)
  • DNA methylation and histone modifications play a central role in the epigenetic regulation of gene expression and cell differentiation. (cipsm.de)
  • This is accomplished by increasing both the interaction strength and number of interactions among participant proteins, which is not necessarily accompanied by a change in expression levels. (nature.com)
  • Gene microarray platforms provide tremendous breadth in surveying the gene expression landscape within a cellular system. (biomedcentral.com)
  • In contrast to genomic-based assays, proteomics provides an attractive platform to profile this regulatory layer of protein activity and differences in the level of protein expression [ 10 ]. (biomedcentral.com)
  • We identified genes whose expression is correlated with the degree of thermotolerance in Saccharomyces cerevisiae by DNA microarray analysis. (springeropen.com)
  • Gene expression profiles of three S. cerevisiae strains showing different levels of thermotolerance were compared, and we chose three of them as candidate genes. (springeropen.com)
  • Among these genes, FMP21 was investigated as a thermotolerance-related gene in S. cerevisiae by comparing the growth at high temperature with the gene expression in eight strains. (springeropen.com)
  • Analyses of genes whose expression is induced by high temperature are important for understanding the mechanisms of thermotolerance, and for the use of the genes in breeding thermotolerant yeasts. (springeropen.com)
  • In the present study, we attempted to identify genes whose expression is correlated with the degree of thermotolerance in S. cerevisiae . (springeropen.com)
  • We compared the gene expression profiles of three S. cerevisiae strains that showed different levels of thermotolerance. (springeropen.com)
  • Differential gene expression analysis identified 32 genes that were differentially expressed in GSC 1123-R compared with GSC 1123-C (false discovery rate? (neuroart2006.com)
  • The data showed an agreement in the expression levels of these genes between the RNA-seq and QRT-PCR analyses (Fig. (neuroart2006.com)
  • We further Poziotinib confirmed that protein expression of G0S2 was higher in GSC 1123-R cells compared with GSC 1123-C cells (Fig. (neuroart2006.com)
  • The expression level of G0S2 protein. (neuroart2006.com)
  • Using bioinformatics analyses, we predicted HNK protein targets from several databases and retrieved the genes differentially expressed in mBCSCs from the GEO database. (hindawi.com)
  • Genes related to the thermotolerance of S. cerevisiae have been identified by DNA microarray analyses (Eisen et al. (springeropen.com)
  • Phylogenetic analyses of the family Trypanosomatidae have been conducted using both 18S rRNA gene sequences and a variety of protein sequences. (biomedcentral.com)
  • By contrast, phylogenetic analyses of available sequences in 42 protein families gene generally supported monophyly of the genus Trypanosoma . (biomedcentral.com)
  • Here we briefly review some of the major results of previous molecular phylogenetic analyses of Trypanosomatidae and present new analyses based on 42 protein families. (biomedcentral.com)
  • Both related proteins also associate with the molecular chaperone heat shock protein-90 (Hsp90) and negatively regulate its ATPase activity and facilitate its association with folliculin. (nih.gov)
  • Compounds 1-4 were tested for their antibacterial activity against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 and for their ability to inhibit the intrinsic ATPase activity of the heat shock protein 90 (Hsp90). (elsevierpure.com)
  • Plastid-specific ribosomal proteins (PSRPs) have been proposed to play roles in the light-dependent regulation of chloroplast translation. (cipsm.de)
  • Here we demonstrate that PSRP1 is not a bona fide ribosomal protein, but rather a functional homologue of the Escherichia coli cold-shock protein pY. (cipsm.de)
  • We will show that phylogenies based on 18S ribosomal RNA (18S rRNA) genes have provided an answer to this question that appears inconsistent with the results of the majority of phylogenies based on available protein sequences. (biomedcentral.com)
  • Since Hsp90 can market the activation of Stat3 , predictably, 17-DMAG could enrich de-repression of p53 accumulation within a Stat3-dependent manner. (cd31-signal.com)
  • 17-DMAG induced an accumulation of p53 protein but not RNA, implicating regulation of p53 turnover. (cd31-signal.com)
  • Hsp90 consumer proteins that influence p53 stability include Mdm2, the E3 ligase that right ubiquitylates and promotes the degradation of p53, Chk1, a downstream kinase of Atm that phosphorylates p53 to disrupt its interaction with Mdm2 and Akt that phosphorylates Mdm2 to enhance p53 accumulation. (cd31-signal.com)
  • Ageing leads to a gradual dysfunction of the proteostasis network and thus to proteome instability due to accumulation of damaged and/or misfolded proteins [ 2 ]. (hindawi.com)
  • In addition, transgenic lines showed upregulation of some important genes involved in plant defense response to oxidative stress. (bvsalud.org)
  • Five generations of selection at 39 °C led larvae to acclimate to this heat stress. (biomedcentral.com)
  • Both the heat-acclimated and unacclimated larvae responded to heat stress via upregulating genes related to sensory organ development and structural constituent of eye lens, whereas the unacclimated larvae also upregulated genes related to structural constituent of cuticle. (biomedcentral.com)
  • Additionally, the unacclimated larvae also adjusted the spliceosome pathway, whereas the heat-acclimated larvae adjusted the biosynthesis of unsaturated fatty acids pathway when encountering heat stress. (biomedcentral.com)
  • Endoplasmic reticulum stress (ERS) is a self-protective mechanism resulting from an imbalance in quality control of unfolded proteins when cells are stressed, which plays an active role in lung cancer development, but the relationship between ERS and the pathological characteristics and clinical prognosis of LUAD patients remains unclear. (biomedcentral.com)
  • However, if the stress on the ER is chronic or severe and quality control of protein folding cannot be met, ER activates its stress receptor, the unfolded protein response, which triggers programmed cell death [ 9 ]. (biomedcentral.com)
  • All these data indicate that diethyl ether anesthesia may partially mimic heat stress in plants through the effect on plasma membrane. (frontiersin.org)
  • this spice is good for making the stressor more efficient at turning on your stress coping genes. (anti-agingfirewalls.com)
  • The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)
  • In all living cells, protein synthesis occurs on ribonucleoprotein particles called ribosomes. (cipsm.de)
  • Ribonucleic acid in protozoa having regulatory and catalytic roles as well as involvement in protein synthesis. (lookformedical.com)
  • Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis. (lookformedical.com)
  • They do this by allowing the proteins to undergo conformational changes, to reach specific subcellular localizations, or to form multimeric complexes [7]. (scientificarchives.com)
  • CRP is a dynamic protein undergoing conformational changes upon activation in inflammatory microenvironments between pentameric and monomeric isoforms. (bvsalud.org)
  • Several molecular mechanisms with antifungal agents have been reported for C. albicans where insertions, deletions, and point mutations in genes codifying target proteins are frequently related to the antifungal drug resistance. (intechopen.com)
  • These results are helpful to understand the molecular mechanism underlying heat acclimation of insects. (biomedcentral.com)
  • Epigenetic modifying enzymes such as histone deacetylases (HDACs), p300, and PRMT1 are recruited by AML1/ETO, the pathogenic protein for t(8;21) acute myeloid leukemia (AML), providing a strong molecular rationale for targeting these enzymes to treat this disease. (ashpublications.org)
  • Activated protein C resistance and low molecular weight lipoprotein (a): dual pathogens for atherothrombosis? (mcmaster.ca)
  • After the pathogenic bacterium Legionella pneumophila is phagocytosed, it injects more than 250 different proteins into the cytoplasm of host cells to evade lysosomal digestion and to replicate inside the host cell. (cipsm.de)
  • Cells express a pool of thousands of different proteins that need to be tightly controlled for proper cellular structure, organization, and function. (hindawi.com)
  • MAIN CONCLUSION: The overexpression of the GmGlb1-1 gene reduces plant susceptibility to Meloidogyne incognita. (bvsalud.org)
  • Thus, based on our data and previous studies, it was hypothesized that constitutive overexpression of the GmGlb1-1 gene can interfere in the dynamics of ROS production and NO scavenging, enhancing the acquired systemic acclimation to biotic and abiotic stresses, and improving the cellular homeostasis. (bvsalud.org)
  • Furthermore, gene overexpression is also frequently associated to antifungal resistance as well as an increase in the activity of proteins that reduce oxidative damage. (intechopen.com)
  • RNA-sequencing analysis showed that the overexpression of differential gene HSP30 increased the transcriptional abundance of ATF1 gene and production of ethyl acetate. (biomedcentral.com)
  • Challenge of rGMCs with 1 or 10 μM HgCl 2 or Pb 2 acetate increased HSPA5 mRNA and protein levels. (bioone.org)
  • These vesicles are carriers of active or non-autonomous function biomolecules, such as proteins, lipids, DNA, mRNA and non-coding regulatory RNA. (spandidos-publications.com)
  • Members of the Alb3/Oxa1/YidC protein family function as insertases in chloroplasts, mitochondria, and bacteria. (cipsm.de)
  • Besides their function in the virus replication cycle, the viral glycoprotein, nucleoprotein, minor matrix protein and polymerase cofactor are viral determinants of pathogenicity, with evasion of the host innate and adaptive immune responses as the main mechanism. (biomedcentral.com)
  • Elucidate the structure, dynamics and functions of intrinsically disordered proteins and protein regions (IDPs/IDRs) and their biological regulation by Post-translational modifications. (upstate.edu)
  • In synthetic biology, the strength of promoter elements is the basis for precise regulation of target gene transcription levels, which in turn increases the yield of the target product. (biomedcentral.com)
  • Hsp26, Hsp78, Hsp82) decreased the production of ethyl acetate, suggesting that the Hsp family was also involved in the regulation of ATF1 gene transcription. (biomedcentral.com)
  • The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. (cipsm.de)
  • Preexisting antivector immunity can severely compromise the ability of Salmonella enterica serovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. (cipsm.de)
  • The changes in transcript level of genes were related to heat acclimation of larvae, especially these genes in sensory organ development, structural constituent of eye lens, and oxidoreductase activity. (biomedcentral.com)
  • It contains most of the cell's genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. (absoluteastronomy.com)
  • The number of galls per plant and the number of egg masses per plant were reduced by up to 85% in transgenic soybean roots, supported by the downregulation of effector genes in M. incognita during parasitism. (bvsalud.org)
  • Similarly, the number of galls per plant, the number of egg masses per plant, and the nematode reproduction factor were reduced by up to 83% in transgenic N. tabacum lines, which was also supported by the downregulation of the Minc00801 effector gene during parasitism. (bvsalud.org)
  • The heat-acclimated larvae induced more DEGs to response to heat shock than the unacclimated larvae. (biomedcentral.com)
  • Fish protein binds more than 90% of the consumed methylmercury so tightly that even the most vigorous cooking methods (eg, deep-frying, boiling, baking, pan-frying) cannot remove it. (medscape.com)
  • An unusual cellular factor potentiates protein-DNA complex assembly between Oct-1 and Vmw65. (mcmaster.ca)
  • Transcriptomic analysis of ZAP-isoform-specific knockout cells revealed uncharacterized host mRNAs targeted by ZAPL/S with broad cellular functions such as unfolded protein response (UPR), epithelial-mesenchymal transition (EMT), and innate immunity. (bvsalud.org)
  • In chapter 2, by using CRISPR/Cas9 gene editing, genetic analysis and biochemical assays, I identified the differential pairings of typical NLR receptor SOC3 with atypical NLR proteins CHS1 or TN2 to guard the homeostasis of the E3 ligase SAUL1. (ubc.ca)
  • Due to independent gene duplications, all organisms possess two isoforms, Oxa1 and Oxa2 except gram-negative bacteria, which encode only for one YidC-like protein. (cipsm.de)
  • Herein, the Minc01696, Minc00344, and Minc00801 putative effector genes were evaluated to assess their importance during soybean and Nicotiana tabacum parasitism by M. incognita. (bvsalud.org)
  • Modulation of chromatin through histone modification is essential in regulating fundamental biological processes such as gene transcription and DNA repair. (ashpublications.org)
  • RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (lookformedical.com)
  • The Biological Chemistry technical symposia at Pacifichem 2015 features 28 sessions covering a myriad of topics, including peptide and protein chemistry, natural products, epigenetics/epigenomics, biocatalysis, and bioengineering. (acs.org)
  • Chloroplast-located proteins which are encoded by the nuclear genome have to be imported from the cytosol into the organelle in a post-translational manner. (cipsm.de)
  • Protein targeting or protein sorting is the mechanism by which a cell transports proteins to the appropriate positions in the cell or outside of it. (absoluteastronomy.com)
  • We introduce the term protein connectivity-based dysfunction (PCBD) to define this mechanism. (nature.com)
  • As a positive regulator of plant immunity, E3 ligase SAUL1 is guarded by NLR protein SOC3. (ubc.ca)
  • Further characterization showed that susa2-2 only suppresses the autoimmunity mediated by either CHS1-SOC3 or TN2-SOC3 paired NLR proteins, indicating that SUSA2 is specifically involved in NLR protein SOC3-mediated immunity. (ubc.ca)
  • Some sequences are similar to those proven to be targets for effective intervention in other protozoan parasites, and include not only proteases, antioxidant enzymes, and heat shock proteins, but also those associated with relict plastids, such as acetyl-CoA carboxylase and methyl erythrithol phosphate pathway components, and those involved in glycan assembly, protein folding/secretion, and parasite-host interactions. (biomedcentral.com)
  • In a virtual screening experiment, chaxamycins A-D (1-4) have also been docked into the ATP-binding pocket in the N-terminal domain of the Hsp90, and the observed interactions are discussed. (elsevierpure.com)
  • Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. (cipsm.de)
  • Most NLRs guard host proteins that are the direct targets of pathogen effectors. (ubc.ca)
  • Cox regression analysis identifies independent prognostic factors for these patients, and enrichment analysis of prognosis-related genes was also performed. (biomedcentral.com)
  • However, the results of many researches proved that excessive transcription levels of target genes actually reduced the yield of the target product. (biomedcentral.com)
  • In this study, promoters PGK1p and TDH3p with different strengths were used to regulate the transcription level of alcohol acetyl transferase encoding gene ATF1 . (biomedcentral.com)
  • The results of Real-time PCR proved that the transcription level of ATF1 decreased rapidly under the control of TDH3p , and the unfolded protein reaction was activated, which may be the reason for the abnormal production caused by the strong promoter. (biomedcentral.com)
  • The fine-tuning and precise control of key gene transcription levels are the important basis for the successful implementation of these strategies. (biomedcentral.com)
  • During parasitism in host plants, M. incognita secretes several effector proteins to suppress the plant immune system, manipulate the plant cell cycle, and promote parasitism. (bvsalud.org)
  • C-reactive protein (CRP), an active regulator of the innate immune system, has been related to COVID-19 severity. (bvsalud.org)
  • CCT, chaperonin containing TCP-1), uses a built-in lid to mediate protein folding in an enclosed central cavity. (cipsm.de)
  • Anfinsen CB (1973) Principles that govern the folding of protein chains. (springer.com)