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  • substrate
  • Using photolithography to shape a polydimethylsiloxane substrate and micropatterning to alter the substrate surface, the researchers developed 3D molds that mimic the natural forces and pressures that cardiomyocytes experience in the heart. (medgadget.com)
  • Additional micro-patterning of the substrate changes the cytoskeleton in the cell and the shape of the nucleus, which cause the genes in the cell to change. (eurekalert.org)
  • microarray
  • miRNA microarray analysis suggests a coordinated and potentially more complex role of multiple miRNA in the regulation of cardiomyocyte quiescence and highlights significant differences between species that may reflect their substantial differences in the timing of this developmental process. (biomedcentral.com)
  • promoter
  • Replication-deficient recombinant adenovirus (ST5) encoding nuclear-targeted β-galactosidase (AdV β-gal) driven by a cytomegalovirus (CMV) promoter 13-16 or cardiomyocyte-specific promotor (mouse α-myosin heavy chain [MHC]) was used. (ahajournals.org)
  • regenerative
  • iPSCs show significant potential for cardiac regenerative medicine, as researchers can use biochemical cues to "program" them into a cardiomyocyte-like cell, which shows regenerative efficacy in damaged cardiac muscle. (medgadget.com)
  • Also, the study suggests that knowing how to control how quickly a cell differentiates as it matures has important implications for regenerative medicine. (genengnews.com)
  • transcription factors
  • The cardiac gene regulatory network (GRN) is controlled by transcription factors and signaling inputs, but network logic in development and it unraveling in disease is poorly understood. (ahajournals.org)
  • Three approaches are currently being explored (i) second generation antisense oligonucleotides (ASOs), (ii) transcription factors fused to novel protein transduction domains, and (iii) epigenetic engineering of ESC by gene-specific DNA methylation or demethylation to limit differentiation to the desired pathway. (cityofhope.org)
  • Researchers
  • Once in this naïve state, the cell has the potential to develop into any cell type in the body, but researchers direct it to develop into a cardiomyocyte. (healthcanal.com)
  • Laid out in a new article published today in Advanced Functional Materials , a team of researchers at Michigan Technological University in collaboration with Harvard Medical School, shows how cardiomyocytes grown in a heart-like environment mature more quickly, have improved functionality and are less likely to be rejected by patients' bodies. (eurekalert.org)
  • chromatin
  • And these genes may come into play, influencing cellular differentiation, if the chromatin is reorganized. (genengnews.com)
  • My laboratory research is broad-based, with an emphasis on gene regulation by chromatin-based mechanisms and changes in the genome that take place during mammalian development. (cityofhope.org)
  • regulation of cardiovascular
  • There are several key genes that are involved in the regulation of cardiovascular health, including sirtuins , AMP -activated protein kinase, mammalian target of rapamycin , and insulin-like growth factor 1 (Figure 1). (ptglab.com)
  • mouse
  • In contrast, the Wingless (Wg)/armadillo pathway (which corresponds to the Wnt/β-catenin pathway in vertebrates) promotes heart formation in Drosophila ( 8 , 9 ), and Wnt/β-catenin signaling induces cardiomyocyte differentiation in a mouse embryonal carcinoma cell line P19CL6 ( 10 , 11 ). (pnas.org)
  • patterns
  • The chamber GRN subsequently decayed with individual genes exhibiting decay patterns unrelated to known patterning boundaries. (ahajournals.org)
  • early
  • Purified early ventricular cardiomyocytes were estimated by immunofluorescence, fluorescence-activated cell sorting, quantitative PCR, microelectrode array, and patch clamp. (biomedcentral.com)