Genes and proteins recombinant proteins. Medical search. Frequent questions

Neither did antibody to the recombinant 37,000 dalton protein react with any treponemal proteins purified by fibronectin affinity chromatography. (bmj.com)
Finally, only sera of patients with primary syphilis possessed appreciable concentrations of antibody to recombinant P2 protein. (bmj.com)
If you can't find the antibody you need, please check CUSABIO protein custom service . (cusabio.com)
The following product was used in this experiment: Carbonic Anhydrase II Recombinant Rabbit Monoclonal Antibody (ARC1451) from Thermo Fisher Scientific, catalog # MA5-38188, RRID AB_2898105. (thermofisher.com)

The recombinant protein in Escherichia coli lysates was labelled P2 (P2 star) to differentiate it from the comigrating adhesin protein called P2. (bmj.com)
Recent studies have shown that it may be possible to produce recombinant lysosomal proteins with microorganisms such as Escherichia coli and Saccharomyces cerevisiae. (wikipedia.org)

We own protein expression technology based on a series of protein expression and purification technology, including protein expression technology shown by Baculovirus outer membrane proteins (OMP), and membrane protein expression technology, etc. (cusabio.com)
Protein production Gene expression Protein purification Host cell protein Young CL, Britton ZT, Robinson AS (May 2012). (wikipedia.org)
The new extracted gene was sequenced and cloned in E. coli, followed by protein purification using a C-terminal His-tag. (bvsalud.org)
Vectors containing the preprotrypsin leader (PPT) sequence direct secretion of FLAG fusion proteins into the culture medium for purification using ANTI-FLAG antibodies, resins, and plates. (sigmaaldrich.com)
A His-tag will be added to each recombinant protein to facilitate protein purification. (usda.gov)
The students will have opportunity to practice several of these methods during laboratory work, including site-directed mutagenesis, recombinant protein production and purification, real-time RT-PCR. (lu.se)
Purification and characterization of two protein kinases acting on the aquaporin SoPIP2;1. (lu.se)

The recombinant proteins will be used as antigens to produce polyclonal antibodies. (usda.gov)
The antibodies will be used for western blots or immunostaining to detect the presence of the Hessian fly proteins in infested wheat tissues. (usda.gov)
Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (antikoerper-online.de)
Within the past 2 years, investigational peptide- and recombinant protein-based serologic assays that can more easily differentiate the antibodies to HTLV-I and HTLV-II have been developed (8,9). (cdc.gov)
135 antibodies against 65 mainly immunoregulatory proteins, we of a multitude of different genes (8, 9). (lu.se)
however, rabbit antibodies raised against the recombinant proteins encoded by the gene of mouse bullous pemphigoid antigen 2 (BP180) are capable of inducing blisters in newborn mice in a complement-dependent manner. (medscape.com)

The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. (lu.se)

Cloning structural genes for Treponema pallidum immunogens and characterisation of recombinant treponemal surface protein, P2 (P2 star). (bmj.com)
Cloning, expression, and characterization of potential immunogenic recombinant porcine deltacoronavirus (PDCoV) membrane protein from a synthetic gene. (authorea.com)
Gene cloning and characterization of a novel recombinant 40-kDa heat shock protein from Mesobacillus persicus B48. (bvsalud.org)
The human glucosamine-6-phosphate deaminase gene: cDNA cloning and expression, genomic organization and chromosomal localization. (nih.gov)

The tyrosinase‐induced polymerization of peptides offers facile access to artificial mussel foot protein analogues. (wikipedia.org)
The use of amino acids, proteins and peptides is not considered to have any environmental impact. (janusinfo.se)
According to the European Medicines Agency guideline on environmental risk assessments for pharmaceuticals (EMA/CHMP/SWP/4447/00),vitamins, electrolytes, amino acids, peptides, proteins, carbohydrates, lipids proteins, vaccines and herbal medicinal products are exempted because they are unlikely to result in significant risk to the environment. (janusinfo.se)

Hence, an expression system performed in E. coli BL21 (DE3) using the vector pET-SUMO with a His-tag was prepared, resulting in a synthetic M gene of 654 pb to produce a recombinant M-PDCoV protein ( r M-PDCoV). (authorea.com)
Compared with traditional protein expression systems, many processes are omitted in vitro E.coli, such as the transformation of plasmids, cell culture, collection, crushing and centrifugation, etc., which greatly improves working efficiency. (cusabio.com)
In addition, salt resistance experiments showed that the survival of recombinant E. coli+DnaJ was 2.1 times more than control cells in 0.5 M NaCl. (bvsalud.org)
Furthermore, the number of recombinant E. coli BL21+DnaJ colonies was 7.7 times that of the control colonies in pH 8.5. (bvsalud.org)
Recombinant Human JNK2 protein(1-424 aa), fused to GST tag, was expressed in E. coli. (creativebiomart.net)
Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)
The drug substance anakinra is a recombinant protein (Interleukin-1 receptor antagonist) produced in E. coli bacteria containing an expression plasmid in which a synthetic gene coding for human IL-1Ra has been inserted.According to the Guideline on the Environmental Risk Assessment of Medicinal Products for Human Use (EMEA 2006), proteins are exempted from environmental risk assessment because they are unlikely to result in significant risk to the environment. (janusinfo.se)

A dilution series of the recombinant Lucia luciferase protein can also be used to determine the linear range of the assay. (invivogen.com)
In the present study, we evaluated the efficiency of protein translation, focusing on the effect of DNA templates of different lengths on cell-free protein synthesis using a luciferase assay. (nature.com)

Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. (origene.com)
The encoded protein is secreted and binds hydroxyapatite with high affinity. (origene.com)
Protein G affinity purified. (enzolifesciences.com)
Affinity tags can reduce merohedral twinning of membrane protein crystals. (lu.se)

If you are looking for recombinants with excellent quality (validated bio-activity, lower endotoxin level and high purity) to support your research, you are in the right place. (cusabio.com)
The purity of Human ACE2 (18-615) Recombinant Protein (hFc-Tag) was determined by densitometry after SDS-PAGE of 1 μg of protein, followed by staining with Coomassie Blue. (cellsignal.com)

A capture IgM EIA (non-quantitative) that incorporates a recombinant measles virus nucleocapsid protein as the antigen is used to detect measles IgM. (cdc.gov)

The constituent proteins are normally synthesised in specialised glands where the epithelial cells are responsible for the biosynthesis. (bioone.org)

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)

Here is the catalog of all recombinant proteins. (cusabio.com)
The ED 50 for this effect is 1-4 μg/mL in the presence of 0.5 ng/mL of Recombinant Mouse IL-22 (Catalog # 582-ML ). (rndsystems.com)
When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7 ) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human ICOS Fc Chimera binds with an ED 50 of 1-6 ng/mL. (rndsystems.com)
The following product was used in this experiment: Human MAP4K5 (KHS1), GST Tag Recombinant Protein from Thermo Fisher Scientific, catalog # PV3682. (thermofisher.com)
In addition to our diverse catalog and semi-custom recombinant protein products, we also offer comprehensive custom recombinant protein expression and production services to meet a variety of research needs. (avivasysbio.com)

We select from GenBank a group of 138 sequences and obtained a consensus PDCoV membrane protein (M-PDCoV) sequence of 216 a.a. (authorea.com)
CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. (fishersci.com)
Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. (prosci-inc.com)

In this study, we showed that the human RFC (hRFC) gene can serve as a novel selectable marker for the overproduction of recombinant proteins. (aspetjournals.org)
In many cases, recombinant human proteins have replaced the original animal-derived version used in medicine. (wikipedia.org)
The prefix "rh" for "recombinant human" appears less and less in the literature. (wikipedia.org)
To facilitate future mutation detection analyses, we have therefore characterized the intron-exon organization of the human keratin 15 and keratin 19 genes. (nih.gov)
The Recombinant Human Lysyl Oxidase Homolog 2 Protein is derived from NS0. (fishersci.com)
The Recombinant Human Lysyl Oxidase Homolog 2 Protein has been validated for the following applications: Enzyme Activity. (fishersci.com)
Binding response kinetics between Human ACE2 (18-615) Recombinant Protein (hFc-Tag) (immobilized) and SARS-CoV-2 Spike (trimeric) (16-1208) Recombinant Protein (8xHis-Tag) #65444 (in solution, at indicated concentrations). (cellsignal.com)
Recombinant human/mouse/rat BMP-2 (355-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. (novusbio.com)
Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. (rndsystems.com)
This gene is localized to human chromosome 3q. (sigmaaldrich.com)
Recombinant Human EBI3(Arg21-Lys229) and p28(Phe29-Pro243) fused with His tag at C-terminal was expressed in NS0. (creativebiomart.net)
The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein. (creativebiomart.net)
The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region. (creativebiomart.net)
The strong human cytomegalovirus (CMV) promoter regulatory region drives constitutive protein expression levels as high as 1 mG/L in COS cells. (sigmaaldrich.com)
To do this, we need to combine the human instructions for how to make the protein with bacterial instructions for where and how much to make. (thetech.org)
The adeno-associated virus (AAV) is one of the most promising viral vectors for human gene therapy. (nature.com)
Recombinant full length human NKX2.2. (enzolifesciences.com)
As an example of protein therapy, a completed phase I/II trial associated recombinant human collagen with wound healing and pain reduction in RDEB. (medscape.com)

The PEP enzyme recognizes the -10 and -35 cis -elements, similar to those found in bacterial promoters whereas the NEP enzyme recognizes the YRTA-motif, which can also be found upstream of several genes with PEP promoters indicating that these genes can be transcribed by both polymerases ( Pfannschmidt and Liere, 2005 ). (frontiersin.org)

Lysates/proteins: 25 µg per lane. (thermofisher.com)

PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit a) is one of the four subunits of the 110kDa catalytic subunit of PI3K protein, which contains a regulatory region of 85kDa. (sigmaaldrich.com)
PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit a) is one of the four catalytic subunits of PI3K protein, which plays an essential role in extracellular growth signaling. (sigmaaldrich.com)
It phosphorylates and activates protein kinase B (PKB) protein. (sigmaaldrich.com)

Silks are composed principally of proteins with a predominance of alanine, serine and glycine and silk proteins are able to undergo irreversible transformations from soluble protein to insoluble fibres. (bioone.org)
If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)

As a result, recombinant lysosomal proteins are usually produced in mammalian cells. (wikipedia.org)
A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. (antikoerper-online.de)
The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)

Several genes encoding putative Hessian fly effector family 31 members have been cloned previously. (usda.gov)

Our selection of CMV vectors provides options for transient or stable expression of 3xFLAG ® fusion proteins. (sigmaaldrich.com)
Xiao, X., Li, J. & Samulski, R.J. Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus. (nature.com)
Collaco, R.F., Cao, X. & Trempe, J.P. A helper virus-free packaging system for recombinant adeno-associated virus vectors. (nature.com)
High-titer recombinant adeno-associated virus production from replicating amplicons and herpes vectors deleted for glycoprotein H. (nature.com)
Zhang, X. & Li, C.Y. Generation of recombinant adeno-associated virus vectors by a complete adenovirus-mediated approach. (nature.com)

Native P2 protein was present on T pallidum surfaces as shown by radioimmunoprecipitation assays with extrinsically labelled organisms. (bmj.com)
Recombinant Lucia Luciferase Protein is a positive control for luciferase reporter assays. (invivogen.com)
This recombinant protein can be used for biological assays. (prosci-inc.com)

Over more than 16 years, CUSABIO has established five systems from prokaryotic to eukaryotic to express the recombinant protein. (cusabio.com)
In addition, after transcription, a wide array of RNA-binding proteins interacts with cis -acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. (frontiersin.org)
The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antikoerper-online.de)

Moreover, PRIN2 and CSP41b form a distinct protein complex in vitro that binds DNA. (frontiersin.org)
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. (novusbio.com)
protein interaction and localization studies in vitro and in vivo. (lu.se)

Bone morphogenetic proteins (BMPs) are osteoinductive substances and have been used in bone regeneration. (bvsalud.org)

This monomeric protein is expressed in CHO cells. (invivogen.com)
For less potent cell lines, protein levels are typically ~0.1 mG/L. The presence of the SV40 replication origin will result in high levels of DNA replication in SV40 replication permissive COS cells. (sigmaaldrich.com)
One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. (joplink.net)
Cell-free gene expression systems have been valuable tools for understanding how transcription/translation can be regulated in living cells. (nature.com)
Gene expression in living cells is strictly self-regulated to ensure that the correct amounts of proteins are made at the most appropriate timing and location for maintaining cellular homeostasis. (nature.com)
Red blood cells contain hemoglobin, a protein that enables them to carry oxygen from the lungs and deliver it to all parts. (msdmanuals.com)

The prin2.2 and csp41b-2 single mutants displayed pale phenotypes, abnormal chloroplasts with reduced transcript levels of photosynthesis genes and defects in embryo development. (frontiersin.org)
Several transcript variants encoding different isoforms have been found for this gene. (origene.com)
Polyadenylation is essential for the stability of the transcript, preventing the mRNA from being the target of posttranscriptional gene silencing (PTGS) via RNA-dependent RNA polymerase 6 (RDR6) in plants ( Luo and Chen, 2007 ). (frontiersin.org)
Through APA, a single gene containing multiple PAS can generate a considerable number of transcript isoforms, thereby producing a highly diversified transcriptome ( Tian and Manley, 2013 ). (frontiersin.org)
Two alternatively spliced transcript variants encoding the same protein have been described for MAP4K5. (thermofisher.com)

Spectroscopic results showed 56% higher carbonic anhydrase activity in the presence of the recombinant DnaJ homolog compared to its absence. (bvsalud.org)

Mellerio also described a recently completed phase 3 trial with introduction of ex vivo gene-corrected keratinocytes, which has been associated with long-term improvements among patients with recessive dystrophic EB (RDEB). (medscape.com)

A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (antikoerper-online.de)

Furthermore, the possible implications to subcellular transporter localization and restoration of MTX sensitivity in drug-resistant tumors by RFC-based gene therapy are discussed. (aspetjournals.org)
The availability of multiple PAS in the same 3' regulatory region enables the inclusion or exclusion of 3' untranslated region (3'UTR) sequences, resulting in transcripts that may differ in particularities involving post-transcriptional processes such as stability, transport and translation ( Mayr, 2016 ), and even protein localization ( Berkovits and Mayr, 2015 ). (frontiersin.org)

Scientists first started making synthetic genes by cutting and pasting parts of different genes together. (thetech.org)

Designing and making synthetic genes has been done for decades, although the technologies have gotten better with time. (thetech.org)
The second is creating a synthetic gene directly from DNA. (thetech.org)
One way to make a synthetic gene is to recombine these instructions. (thetech.org)
The search for materials presenting characteristics similar to those achieved with autograft, aiming to reduce morbidity in procedures of restoration of lost bone structures, allowed research to develop toward synthetic materials, such as the rhBMP-2, which is the main morphogenetic protein inducer of bone tissue. (bvsalud.org)

Treponemal proteins ranging in molecular weight from 37,000 daltons to 120,000 daltons were identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting of phage lysate proteins. (bmj.com)
The mature protein has an approximate molecular weight of 24 kDa. (invivogen.com)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)

This risk assessment was not dependent on the type of adju- decoding patterns of immunoregulatory serum proteins could re- vant therapy received by the patients. (lu.se)

Non-interacting proteins or other molecules will be washed off with buffers. (usda.gov)
The molecules bound to recombinant family 31 proteins will be eluted and will be identified via liquid chromatography/mass spectrometry quadrupole time of flight (LC-Q-TOF) instrumentation. (usda.gov)
Proteins are molecules that are involved in almost everything a cell does. (thetech.org)

Aviva Systems Biology has been providing high-quality protein products to the scientific community for over 10 years. (avivasysbio.com)
The course presents methods to express genes, which have products that are known, unknown, or not well characterised, and to analyse the gene products. (lu.se)

We show here that PLASTID REDOX INSENSITIVE 2 (PRIN2) and CHLOROPLAST STEM-LOOP BINDING PROTEIN 41 kDa (CSP41b), two proteins identified in plastid nucleoid preparations, are essential for proper plant embryo development. (frontiersin.org)

Activity of recombinant Lucia luciferase protein determined by measuring Relative Light Units (RLU) in a luminometer using QUANTI-Luc™ . (invivogen.com)
Here we report the effect of the length of linearized reporter DNAs encoding the firefly luciferase gene so as to exclude the influence of supercoiling. (nature.com)

Genetic Characterisation of Plasmodium falciparum Isolates with Deletion of the pfhrp2 and/or pfhrp3 Genes in Colombia: The Amazon Region, a Challenge for Malaria Diagnosis and Control. (cdc.gov)

The stability and function of the recombinant DnaJ protein under salt and pH stress conditions were evaluated. (bvsalud.org)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
The 3' regulatory regions have a great diversity of cis -regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. (frontiersin.org)
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (joplink.net)

Lysosomal proteins: Lysosomal proteins are difficult to produce recombinantly due to the number and type of post-translational modifications that they have (e.g. glycosylation). (wikipedia.org)
Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35-45 kDa secreted glycoprotein in the type II cytokine receptor family (CRF). (rndsystems.com)
The region that contains Hdic will be compared between wild-type WGRC42 and its gene-edited, susceptible mutants. (usda.gov)

These include both commercially available proteins (for example most of the enzymes used in the molecular biology laboratory), and those that are generated in the course specific research projects. (wikipedia.org)
Based on the results, DnaJ from the M. persicus can potentially be employed for improving the functional features of enzymes and other proteins in various applications. (bvsalud.org)
Scientists can take advantage of this by using proteins found in bacteria called "restriction enzymes. (thetech.org)

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. (nih.gov)

The present study reports the recognition and characterization of the gene encoding the co-chaperone DnaJ in the halophilic strain Mesobacillus persicus B48. (bvsalud.org)

Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris. (lu.se)

The reports on base sequences of spider silk protein genes have gained importance as the mechanical properties of silk fibers have been revealed. (bioone.org)

Mutations in keratin genes have been documented in many disorders of the skin, nails, hair, and mucous membranes. (nih.gov)
Frequency of mutations in PIK3CA gene is higher in tumors without lymph node metastasis, than those with in gastric cancer. (sigmaaldrich.com)
Thus, it might be essential to evaluate the mutations in this gene to determine cetuximab treatment in gastric cancer patients. (sigmaaldrich.com)
Multiple platforms for gene editing have been described with the goal of simply excising pathogenic mutations or antisense oligonucleotides for sustained or permanent control of EB expression. (medscape.com)
Mellerio described efforts to use an aminoglycoside to circumvent nonsense mutations that produce messenger RNA decay and impaired production of the proteins that prevent EB. (medscape.com)

Cystatins are proteins that inhibit cysteine proteases. (wikipedia.org)

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. (novusbio.com)

Values on y-axis indicate binding response signals recorded for each protein in solution at the indicated concentration. (cellsignal.com)
Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20? (joplink.net)
We used three different conditions: the same luc -gene concentration (Fig. 1 d), the same nucleotide-unit concentration (Fig. 1 e), and a combination of both (the same luc -gene and nucleotide-unit concentration adjusted by the addition of noncoding DNA, nc 2.6k) (Fig. 1 f). (nature.com)

In order to investigate the functional role of miR-210 during TDI sensitization, BALBc mice were dermally exposed to TDI (0.5-4% v/v) and gene and protein expression were evaluated in the draining lymph nodes (dLN) and ears using RT-PCR and Western blot/ flow cytometry, respectively. (cdc.gov)
Clinical evidence is limited, but Mellerio suggested that the theoretical potential for eliminating the source of abnormal transcription is the restoration of functional proteins essential for reversing skin fragility. (medscape.com)

It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12). (creativebiomart.net)

Anatomy16

Organisms28

Diseases1

Chemicals and Drugs95

Analytical, Diagnostic and Therapeutic Techniques and Equipment52

Phenomena and Processes72

Disciplines and Occupations1

Technology, Industry, Agriculture4

Information Science4

Health Care5

Antibody4

Escherichia2

Purification7

Antibodies6

Saccharomyces1

Cloning4

Peptides3

Coli7

Assay2

Affinity4

Purity2

Nucleocapsid protein1

Biosynthesis1

ELISA1

Catalog5

Membrane protein3

Human18

Bacterial1

Lysates1

Kinase3

Soluble2

Mammalian3

Putative1

Vectors5

Assays3

Eukaryotic3

Vitro3

Substances1

Cells6

Transcript5

Homolog1

Vivo1

Yeast1

Localization2

Different genes1

Synthetic4

Molecular weight3

Serum proteins1

Molecules3

Products2

Preparations1

Luciferase2

Characterisation1

Stability4

Type3

Enzymes3

Carbonic anhydrase1

Characterization1

Enhances1

Sequences1

Mutations5

Proteases1

Morphogenetic protein1

Concentration3

Functional2

Subunit1