• As a largest plant-specific transcription factor family, NAC domain proteins play an important role in plant development and regulation of abiotic stress tolerance. (frontiersin.org)
  • Here, we discovered that ribotoxic stress induces a profound reorganization of NSs with enhanced recruitment of factors required for splice-site recognition, including the RNA-binding protein TIAR, U1 snRNP proteins and U2-associated factor 65, as well as serine 2 phosphorylated RNA polymerase II. (bvsalud.org)
  • Nanos are RNA-binding proteins playing crucial roles in germ cell development and maintenance. (nature.com)
  • The Nanos proteins family regroups highly conserved RNA-binding proteins in higher eukaryotes implicated in germ cell development and maintenance. (nature.com)
  • In mice, rapamycin treatment increases expression of histone proteins and Wdfy3 transcription, and alters chromatin organisation in the small intestine, suggesting the mTORC1-histone axis is at least partially conserved in mammals and may offer new targets for anti-ageing interventions. (sdbonline.org)
  • The results, compiled in the Blood Proteoform Atlas (BPA), indicate that proteoforms better describe protein-level biology and are more specific indicators of differentiation than their corresponding proteins, which are more broadly expressed across cell types. (stanford.edu)
  • Weighted gene correlation network analysis (WGCNA) can identify groups of biologically related proteins using only protein intensity values by constructing protein correlation networks. (stanford.edu)
  • Polycomb Group (PcG) proteins are a family of protein complex that regulate gene expression, especially repressing gene transcription [ 1 ]. (hindawi.com)
  • Alternative splicing of gene can generate multiple transcripts and proteins to regulate tissue and organ development [ 17 ]. (hindawi.com)
  • Bortoluzzi S, Alessi FD, Romualdi C, Danieli GA: Differential expression of genes coding for ribosomal proteins in different human tissues. (karger.com)
  • Therefore, the study of IBD must be faced exploiting an integrated and multilevel approach, ranging from genes, transcripts and proteins to pathways altered in affected tissues, and carefully considering their regulatory mechanisms, which may intervene in the pathology onset. (biomedcentral.com)
  • Extensive evidence has revealed that the core clock machinery involves "clock genes" and "clock proteins" functioning as molecular cogs. (bioone.org)
  • The cartilage-specific localization pattern of these novel TGF-β superfamily members, which contrasts with the more ubiquitous presence of other BMP family members, suggests a potential role for these proteins in chondrocyte differentiation and growth of long bones. (elsevierpure.com)
  • In the central dogma RNA acts as a simple intermediary between the DNA that carries the genetic information and the proteins that define the whole variety of biological processes in the cell. (biomedcentral.com)
  • The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. (cancerindex.org)
  • We evaluated a next generation therapeutic peptide, HTL-001, capable of targeting HOX gene over-expression in GBM by disrupting the interaction between HOX proteins and their co-factor, PBX. (biomedcentral.com)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • The CmNAC1-GFP fusion protein was transiently expressed in tobacco leaves for subcellular localization analysis, and we found that CmNAC1 is localized in the nucleus. (frontiersin.org)
  • OfGT3/42/46 were mainly localized in the nuclei and exhibited no transcriptional activities based on the analysis of the subcellular localization and transcriptional activity assay. (biomedcentral.com)
  • The subcellular localization of EZH2 protein was predicted by using different predictors (CELLO, Euk-mPLoc, WoLF PSORT, and TargetP). (hindawi.com)
  • In this study, 15 turbot MAPKs (SmMAPKs) were identified throughout the whole genome, and their basic chemical and physical properties and subcellular localization were illustrated. (figshare.com)
  • Differential expression patterns of specific candidate genes associated with water deficit stress were confirmed using Real time quantitative polymerase chain reaction (RT-qPCR). (usda.gov)
  • DNA-directed RNA polymerase I subunit RPA34 is an enzyme that in humans is encoded by the CD3EAP gene. (wikipedia.org)
  • In today's research laboratory, two common ways that gene expression is assessed is by using quantitative polymerase chain reaction techniques (QTPCR) for monitoring expression of smaller numbers of genes, and by employing DNA microarrays for the highly parallel monitoring of thousands of genes (or the whole transcriptome). (ddw-online.com)
  • Indeed, bacterial genomes carry, on average, from 78% (for genomes containing the polymerase PolC) to 58% (for the other genomes) of their genes on the leading strand [ 3 ]. (biomedcentral.com)
  • The most generally accepted hypothesis is that co-directional collisions on the leading strand have a weaker effect on RNA polymerase processivity than the head-on collisions occurring on the lagging strand. (biomedcentral.com)
  • Degenerate oligonucleotide primer sets derived from the highly conserved carboxyl-terminal region of the BMP family were designed and used in reverse transcription-polymerase chain reactions with poly(A) + RNA from articular cartilage as template to determine which BMPs are produced by chondrocytes. (elsevierpure.com)
  • Human genetic variants that introduce an AG into the intronic region between the branchpoint (BP) and the canonical splice acceptor site (ACC) of protein-coding genes can disrupt pre-mRNA splicing. (bvsalud.org)
  • Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. (nih.gov)
  • We studied by in situ hybridization and RNA blots the pattern of 9E3 mRNA distribution in the wings of normal, wounded, and RSV-infected newly hatched chicks. (silverchair.com)
  • In Paper III , we present a protocol for performing spatial mRNA genome-wide expression profiling of FFPE tissue specimens. (kth.se)
  • Furthermore, we found homogenous and recurrent iCNV profiles in the high-grade tumors of relapse patients and identified expression of gene SPP1 in the tumor stroma as a potential prognostic mRNA marker in pediatric brain tumor relapse patients. (kth.se)
  • Subsequently, we detected the expression of EZH2 on mRNA level and protein level in two different embryonic development stages (65-dpc and 90-dpc) via qRT-PCR and western blots. (hindawi.com)
  • Our analysis showed that mRNA abundances, gene organization (operon) and gene essentiality are correlated in Buchnera (i.e., the most expressed genes are essential genes organized in operons) whereas no link between mRNA abundances and gene strand bias was found. (biomedcentral.com)
  • In-depth analysis revealed that lncRNAs is an extensive and very heterogenic group from mRNA-like transcripts to circular RNAs. (biomedcentral.com)
  • E) Expression of pqbp1 and ncam mRNA in the neural plate of early neurula embryos. (xenbase.org)
  • In particular, ribotoxic stress causes targeted excision of retained introns from pre-mRNAs of immediate early genes (IEGs), whose transcription is induced during the stress response. (bvsalud.org)
  • However, it has been proven that more than 62% of genomic DNA serves as a template for transcription, which indicates that there are abundant non-coding RNAs (ncRNAs) in human transcriptome [ 2 , 3 ]. (ijbs.com)
  • SncRNAs are less than 200 nt in length, consisting of microRNAs (miRNAs), Piwi-interacting RNAs (piRNAs), endogenous small interfering RNAs (endo-siRNAs or esiRNAs) and transcription initiation RNAs (tiRNAs) [ 6 ]. (ijbs.com)
  • Floral inducers including FLOWERING LOCUS T (FT), FD, SUPPRESSOR OF CONSTANS 1 (SOC1), and LFEAY (LFY) activate the transcription of the majority of genes floral genes, promoting Arabidopsis bolting and flowering ( Johansson and Staiger, 2015 ). (frontiersin.org)
  • Moreover, transcriptomic analyses have demonstrated that, in free-living bacteria, gene transcription levels and chromosomal organization are mutually influenced. (biomedcentral.com)
  • The asymmetry of the distribution of the genes between the two strands of DNA is explained as being a means of minimizing interruptions in gene transcription due to collisions between the DNA and RNA polymerases. (biomedcentral.com)
  • Gene organization in the bacterial chromosome has very well known effects on transcription and a standard example is the organization of genes into operons, which allows for a sophisticated regulation of gene expression [ 9 ]. (biomedcentral.com)
  • Genes contributing to the similarity include ribosomal protein (RP) genes as well as genes involved in transcription, translation and cell division. (karger.com)
  • We predict several transcription factors to regulate differentially expressed genes between mLT-HSCs and other LT-HSC subsets. (biorxiv.org)
  • Dirk Schübeler has done important work in epigenomics and regulatory genomics, in particular with the dynamics of genome-wide methylation patterns, their role in gene regulation, and the interplay between chromatin state and the actions of transcription factors. (unibas.ch)
  • The HOX genes encode a family of homeodomain-containing transcription factors that play important roles in the early embryo, including the establishment of cell and tissue identity, and the regulation of cell proliferation, differentiation, and survival [ 12 ]. (biomedcentral.com)
  • Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
  • Single-cell RNA-seq unmasked the existence of two subsets within the LT-HSC population that was apparent upon stimulation but not steady-state. (biorxiv.org)
  • This manuscript describes an RNA sequencing project to measure global gene expression patterns in the root tissues of field grown upland cotton under water deficit stress. (usda.gov)
  • A total of 1,530 transcripts were differentially expressed between well-watered and water-deficit stressed root tissues, in patterns confirming the accuracy of this technique for future studies in cotton genomics. (usda.gov)
  • Hence, this study was conducted to examine the changes in PAL activity and gene expression patterns in relation to texture in the edible branchlet tissues during early postharvest handling. (scialert.net)
  • Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
  • Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
  • Localization of 9E3/CEF-4 in avian tissues: expression is absent in Rous sarcoma virus-induced tumors but is stimulated by injury. (silverchair.com)
  • The abundant expression of 9E3 in normal tissues indicates that the product of this gene plays a normal physiological role in tissues growing by cell division, perhaps as a growth regulator. (silverchair.com)
  • Finally, expression in conjunction with high collagen levels and in wounded tissues may point to a role in wound response and/or repair, possibly via alteration of extracellular matrix. (silverchair.com)
  • The RNA-seq data suggested that the OfGT s have specific expression patterns in different tissues, and some were induced by salt stress. (biomedcentral.com)
  • Here, we identified the coding sequence of the EZH2 gene and characterized its expression pattern in fetal tissues of Duroc pigs at 65- and 90-day postcoitus (dpc). (hindawi.com)
  • EZH2 was ubiquitously expressed in the fetal tissues of two time points with different expression levels. (hindawi.com)
  • These results validated a different transcript in pigs and characterized its expression profile in fetal tissues of different gestation stages, which indicated that EZH2 played important roles during porcine embryonic development. (hindawi.com)
  • Expression patterns of MAPKs in distinct tissues and under diverse abiotic and biotic stresses were examined using the published available RNA-seq data sets. (figshare.com)
  • Hence, it means that human testis could also play a crucial role in human speciation if these two tissues exhibit similar gene expression patterns. (karger.com)
  • Determining the large scale anatomy of gene expression patterns of various human tissues could draw a more convincing conclusion, and better our understanding of the correlation/inter-correlation among different tissues. (karger.com)
  • Among the 17 tissues, the highest similarity in gene expression patterns was between human brain and testis, based on DDD and clustering analysis. (karger.com)
  • Northern analyses showed that both genes are predominantly expressed in cartilaginous tissues. (elsevierpure.com)
  • This was possible by comparing expression patterns in stromal cells surrounding the tumors with cells of similar tissues to identify unique patterns. (biochain.com)
  • pqbp1 and wbp11 genes are similarly expressed in mesoderm and neural tissues during Xenopus development. (xenbase.org)
  • Considering the spatial proximity of cells within a tissue sample alongside differential expression analysis offers a more comprehensive picture of disease development, key driver mutations, and possible therapeutic targets. (biochain.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Missense mutations in the splicing factor gene PRPF31 cause a dominant form of retinitis pigmentosa (RP11) with reduced penetrance. (molvis.org)
  • This list continues to expand, and, as of this writing, specific mutations listed on OMIM are known for 7 autosomal dominant LGMDs and 23 autosomal recessive LGMDs. (medscape.com)
  • Overlap exists with Congenital Muscular Dystrophy (CMD) as several gene mutations can cause both a LGMD and CMD phenotype. (medscape.com)
  • These diseases are discussed here in part because mutations in 2 genes can present with either an LGMD or a myofibrillar myopathy phenotype. (medscape.com)
  • Our work constitutes the first application of the newly published diploid D5 Gossypium raimondii sequence in the study of tetraploid AD1 upland cotton RNA-seq transcriptome analysis. (usda.gov)
  • Notably, circRNAs display cell-type, tissue-type and developmental-stage specific expression patterns in eukaryotic transcriptome, which reveals their significant regulatory functions in gene expression. (ijbs.com)
  • They can bind to the 3′ untranslated region (3′UTR) of target messenger RNAs (mRNAs) in order to regulate gene expression post-transcriptionally with no sequence specificity but by potential electrostatic interactions with the phosphate backbone of RNA 2 , 3 . (nature.com)
  • Histones H3/H4 regulate expression of an autophagy cargo adaptor Blue Cheese ( Bchs (WDFY3 in mammals), increased expression of which in enterocytes mediates increased H3/H4-dependent healthy longevity. (sdbonline.org)
  • See the in situ hybridization pattern in Kohara NextDB . (nih.gov)
  • In situ hybridization and immunostaining of sections from human embryos showed that CDMP-1 was predominantly found at the stage of precartilaginous mesenchymal condensation and throughout the cartilaginous cores of the developing long bones, whereas CDMP-2 expression was restricted to the hypertrophic chondrocytes of ossifying long bone centers. (elsevierpure.com)
  • An RNA-Seq experiment was performed using field grown cotton plants subjected to different water supplies (well-watered and naturally rain fed) to identify differentially expressed transcripts under water-deficit stress. (usda.gov)
  • Additionally, putative sequence based genome localization of differentially expressed transcripts has detected A2 genome specific gene expression under water-deficit stress. (usda.gov)
  • Hat1 function and the presence of these acetyl marks are not required for the nuclear transport of histone H4 as histone variant His4r retained its nuclear localization both in Hat1 mutants and in His4r-K5R-K12R double point mutants. (sdbonline.org)
  • Generally, lncRNA has a lower expression level, their expression is more tissue-specific, and the majority of lncRNA has nuclear localization. (biomedcentral.com)
  • Therefore, a clearer understanding capsid expression was analyzed by immunofluorescent assay of WNV is necessary in order to identify new strategies to treat in HeLa, 293-T, RD, or SH-SY5Y cells by using anti-His tag or prevent this viral infection (3). (cdc.gov)
  • In Paper II , we present an assay for spatial RNA quality evaluation, used to estimate the success for tissue specimens before proceeding with more expensive spatial sequencing methods. (kth.se)
  • Gen-Probe RNA test, interferon-gamma assay, urine-based antigen test). (cdc.gov)
  • Microglia cells have been implicated to play an important role in the tumor microenvironment, and we found spatial co-localization of microglia and epithelial-to-mesenchymal transition (EMT) signatures in our patient cohort. (kth.se)
  • Since identification of EZH2 in the research of protooncogene product Vav [ 3 ], studies have shown that EZH2 is highly expressed in tumorigenesis, which regulates the expression of tumor suppressor genes, such as in breast cancer, prostate cancer, and lung cancer [ 4 - 6 ]. (hindawi.com)
  • The present invention relates to methods, kits and systems for predicting the benefit from inclusion of taxane in a chemotherapy regimen based on the measurements of gene expression levels in tumor samples of breast cancer patients. (justia.com)
  • One particular gene of interest ( NKX6.3 ) was found by IHC and qPCR to be expressed in DGAST cells but not in normal tissue - suggesting it plays a role in tumorigenesis or precursor events in the tissue prior to tumor formation. (biochain.com)
  • Researchers at the National Institute of Environmental Health Sciences aimed to identify a set of genes whose expression patterns can distinguish diverse tumor types. (rna-seqblog.com)
  • The sequence of this gene is defined by 16 cDNA clones and 18 elements defined by RNA-seq, some from mixed (seen 7 times), embryo (5), l2 (once). (nih.gov)
  • The sequence analysis of the 32-35,000 predicted genes indicates the there may be as many as 1,000 kinase or kinase-like genes. (ddw-online.com)
  • We present single cell RNA sequence data from 1.3m peripheral blood mononuclear cells from 982 healthy human subjects. (iscb.org)
  • For example, XIST (X-inactive specific transcript), one of the first described lncRNAs has a low level of sequence conservation, but a highly conserved function across placental mammals - inactivation of the X chromosome [ 5 ]. (biomedcentral.com)
  • PCR-amplified mycobacterial genes are sequence-specifically captured on microspheres, labeled by magnetic nanoprobes, and detected by nuclear magnetic resonance. (cdc.gov)
  • AceView: gene:dpy-21, a comprehensive annotation of human, mouse and worm genes with mRNAs or ESTsAceView. (nih.gov)
  • This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones. (nih.gov)
  • Accurate alignment of transcribed RNA to reference genomes is a critical step in the analysis of gene expression, which in turn has broad applications in biomedical research and in the basic sciences. (bvsalud.org)
  • We performed AGAIN on our exome/genomes database of patients with severe infectious diseases but without known genetic etiology and identified a private homozygous intronic AG-gain variant in the antimycobacterial gene SPPL2A in a patient with mycobacterial disease. (bvsalud.org)
  • 5) qPCR for confirmation of differential gene expression. (biochain.com)
  • RNA sequencing followed by differential expression analysis was used to identify genes with high expression in DGASTs vs. other GI tumors. (biochain.com)
  • Once target genes were identified, IHC, qPCR and DSP were performed to explore differential expression in BG vs. DGAST tumors. (biochain.com)
  • The physiological properties of most organisms, from cyanobacteria to human, display a circadian (Latin circa dies , or 'about a day') pattern of activity, which is regulated by an endogenous circadian clock. (bioone.org)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • Recent developments in high-throughput sequencing technologies and microarray techniques enable scientists to conduct comprehensive analyses on the abundance, composition, expression profile and molecular function of circRNAs [ 16 ]. (ijbs.com)
  • Beyond the variety of molecular implementations of such patterns, we emphasize the unitary principles associated with them, across scales in space and time, within a general mechanochemical framework. (mpi-cbg.de)
  • Our goal is to convey a picture of pattern formation that draws attention to the principles rather than solely to specific molecular mechanisms. (mpi-cbg.de)
  • At the molecular level, knockdown of PQBP1 in Xenopus animal cap explants inhibits target gene induction by FGF but not by BMP, Nodal or Wnt ligands, and knockdown of either PQBP1 or WBP11 in embryos inhibits expression of fgf4 and FGF4 -responsive cdx4 genes. (xenbase.org)
  • 1 The study aim was to identify potential gene drivers and unique signaling pathways of DGASTs. (biochain.com)
  • The non-specific lethal complex regulates genes and pathways genetically linked to Parkinson's disease. (medscape.com)
  • Limb-girdle muscular dystrophy (LGMD) protein defects occur in several pathways involved in the biologic function of muscle and can be divided into groups based on cellular localization. (medscape.com)
  • Additionally, a computational pipeline was used to classify the putative genome localization of genes identified by the RNA sequencing evaluation. (usda.gov)
  • The completion of the sequencing of the human genome has uncovered 32,000 to 35,000 predicted genes, offering the prospect for many more drugable targets. (ddw-online.com)
  • DSP enables "visualization" of spatial context in samples that is otherwise missed by bulk RNA sequencing. (biochain.com)
  • Studies of human genome demonstrate that protein-coding genes only occupy less than 2% of the entire genome [ 1 ]. (ijbs.com)
  • We illustrate where such mechanisms of pattern formation arise in biological systems from cellular to tissue scales, with an emphasis on morphogenesis. (mpi-cbg.de)
  • Both genes are expressed in the nascent mesoderm and neurectoderm , and morpholino knockdown of either causes defects in differentiation and morphogenesis of the mesoderm and neural plate . (xenbase.org)
  • Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
  • the gene expression profile during B lymphoid differentiation. (lu.se)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • We showed that the method is capable of measuring high RNA quality in tissue areas of both high and low cell density and that the spatial RNA integrity patterns are reflected in spatial transcriptomics data. (kth.se)
  • Finally, our results show the existence of spatial periodic transcriptional patterns in the genome of Buchnera . (biomedcentral.com)
  • We use Arabidopsis for forward genetic screens to identify genes important in the response to the wound-induced C6-volatile E -2-hexenal. (uva.nl)
  • We also aim to identify genes that are specifically regulated by E -2-hexenal using a transcriptomics approach. (uva.nl)
  • Analysis of gene expression can include measuring expression levels of a small set of genes to whole genome expression monitoring. (ddw-online.com)
  • The two successive nanos gene duplications may result from the second and third whole genome duplication events at the basis of Vertebrates and Teleosts respectively. (nature.com)
  • In teleostean fish, two nanos1 genes were generated, probably during the Teleost specific whole genome duplication (3R). (nature.com)
  • The second group includes rRNAs and long non-coding RNAs (lncRNAs), which to date are very poorly functionally annotated. (biomedcentral.com)
  • According to the GENCODE project one third of all human lncRNAs genes are primate-specific [ 4 ]. (biomedcentral.com)
  • In Vertebrates, Nanos paralogous genes were associated to partial redundancies and specific functional evolutions. (nature.com)
  • Consequently, it lays a foundation for further functional characterizations of these genes. (biomedcentral.com)
  • The systematically described collection of microbes is known as the microbiota, while the term microbiome includes their pool of functional genes. (encyclopedia.pub)
  • No specific treatment is available for any of the LGMD syndromes, though aggressive supportive care is essential to preserve muscle function, maximize functional ability, and prolong life expectancy. (medscape.com)
  • This showed that non-coding RNAs are a lot more abundant than it was considered before. (biomedcentral.com)
  • These results suggest a role for DNA replication in patterning epigenetic information in asymmetrically dividing cells in multicellular organisms. (sdbonline.org)
  • The aim of this study was to examine whether Annexin A2 and S100A10 expression can be used as prognostic markers for epithelial ovarian cancer (EOC). (cancerindex.org)
  • Thus, we bridge a gap between traditional tissue preservation methods and novel gene technologytools. (kth.se)
  • Elevated expression of H3/H4 in intestinal enterocytes in Drosophila alters chromatin organization, induces intestinal autophagy through transcriptional regulation, prevents age-related decline in the intestine. (sdbonline.org)
  • This study shows that inhibition of mTORC1 by the lifespan-extending drug rapamycin increases expression of histones H3 and H4 post-transcriptionally, through eIF3 -mediated translation. (sdbonline.org)
  • Profiling 2 to 4 single cells per patient and measuring gene-specific expression from targets previously associated with metastatic breast cancer supports the use of our protocol as a diagnostic tool. (kth.se)
  • The use of cell lines does, however, introduce a risk the immunoglobulin (Ig)-secreting plasma cell in the spleen, of obtaining cell line-specific features as a result of the trans- gut, or BM [1, 2]. (lu.se)
  • This method was used to measure the number of copies of repeated genes such as rRNA genes (2) and to measure whether specific genes were under-replicated during the replication process used in forming polytene chromosomes (3). (ddw-online.com)
  • The OfGT s were found to be located randomly on the 20 O. fragrans chromosomes, and an analysis of gene replication events indicated that the OfGT gene family underwent strong purification selection during the evolutionary process. (biomedcentral.com)
  • When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)
  • (12 ). However, the versatility of the system allows the use of any microRNA of choice, including neuron-specific microRNAs ( 14 ). (lu.se)
  • Based on phylogenetic and synteny analyses, this study reveals that nanos1 gene has undergone multiple duplications and gene copies losses in Vertebrates. (nature.com)
  • Emma Lundberg's group has a wealth of experience in using microscopy to yield biological images that paint a picture of this cell-to-cell heterogeneity of protein expression information, and joining her lab has deepened my expertise in integrating datasets to perform innovative analyses of single-cell protein expression. (stanford.edu)
  • In many cases, the disease gene encodes a component of phototransduction or allied processes with expression confined to the retina, for example the rod visual pigment (reviewed in [ 1 ]), or peripherin-RDS [ 2 ], but not all RP genes show such a restricted pattern of expression. (molvis.org)
  • HOX gene dysregulation is now a widely recognised feature of many malignancies. (biomedcentral.com)
  • In this study we have focused on HOX gene dysregulation in GBM as a potential therapeutic target in a disease with high unmet need. (biomedcentral.com)
  • We show significant dysregulation of these developmentally crucial genes and specifically that HOX genes A9, A10, C4 and D9 are strong candidates for biomarkers and treatment targets for GBM and GBM cancer stem cells. (biomedcentral.com)
  • The transcriptional patterns in each parasite are clonally transmitted over multiple generations of asexual growth, with stochastic switches between the active and silenced states occurring at low frequency ( Figure 1A ). (plos.org)
  • The avian gene 9E3/CEF-4, a member of the superfamily of genes that includes KC and gro, is expressed abundantly in exponentially growing cultures of chick embryo fibroblasts (CEFs) and at high levels in CEFs transformed with Rous sarcoma virus (RSV). (silverchair.com)
  • RNA-seq analysis of embryos indicate that in Hat1 mutants over 2000 genes are dysregulated and the observed transcriptional changes imply a delay in the developmental program of gene expression (Varga, 2019). (sdbonline.org)
  • Human CAST genome location and CAST gene details page in the UCSC Genome Browser. (wikipedia.org)
  • Human CD3EAP genome location and CD3EAP gene details page in the UCSC Genome Browser. (wikipedia.org)
  • AGAIN identified 350 AG-gain variants from the Human Gene Mutation Database, all of which alter splicing and cause disease. (bvsalud.org)
  • CircRNAs exhibit altered expression under pathological conditions and are strongly associated with the development of various human diseases. (ijbs.com)
  • I hope to extend this towards analyzing single-cell proteoform expression, understanding the heterogeneity and flux between these proteoforms in space and time, and digging into the fundamental insights about human biology these data may reveal. (stanford.edu)
  • Here, we present the primary structures of ~30,000 unique proteoforms, nearly 10 times more than in previous studies, expressed from 1690 human genes across 21 cell types and plasma from human blood and bone marrow. (stanford.edu)
  • Previous data has reported similarity between human brain and testis gene expression patterns. (karger.com)
  • Present results provide evidence to support the proposal that human testis and brain share the highest similarity of gene expression patterns. (karger.com)
  • Human genes altered in IBD have been collected from literature, paying particular interest for the immune system alterations prompted by the interaction with the gut microbiome. (biomedcentral.com)
  • The resource provides a section devoted to human genes identified as altered in IBD, which can be queried at different biomolecular levels and visualised in gene-centred report pages. (biomedcentral.com)
  • The IBDsite is an attempt to group available information regarding human genes and microbial aspects related to IBD, by means of a multilevel mining tool. (biomedcentral.com)
  • Neither gene was detectable in the axial skeleton during human embryonic development. (elsevierpure.com)
  • Thousands of long non-coding RNA (lncRNA) genes are annotated in the human genome. (biomedcentral.com)
  • No unspliced RHO transcripts could be detected in RNA from human retina. (molvis.org)
  • Cell-type specific and multiscale dynamics of human focal seizures in limbic structures. (medscape.com)
  • The results implied that the OfGT genes were related to abiotic and hormonal stress responses in O. fragrans , and that the OfGT3/42/46 genes in particular might play crucial roles in responses to salt stress. (biomedcentral.com)
  • The mitogen-activated protein kinase (MAPK) gene family performs crucial roles in cell division, migration, development, apoptosis, inflammatory response, and abiotic and biotic stress responses. (figshare.com)
  • Remarkably, the transient transformation results showed lower MDA content and increased expression of ROS-related genes NbAPX in transgenic plants, which implying OfGT3/42/46 may improve the salt tolerance of tobacco. (biomedcentral.com)
  • These data will facilitate efforts to understand the complex responses governing transcriptomic regulatory mechanisms and to identify candidate genes that may benefit applied plant breeding programs. (usda.gov)
  • Blencowe's research team studies the mechanisms by which genes are regulated and coordinated to provide critical functions in mammalian cells. (unibas.ch)
  • A promoter cis -elements analysis showed that all the OfGT genes contained multiple abiotic and hormonal stress-related cis -elements. (biomedcentral.com)
  • Lack of Hat1 results in the near complete loss of histone H4 lysine (K) 5 and K12 acetylation in embryos, indicating that Hat1 is the main acetyltransferase specific for these marks in this developmental stage. (sdbonline.org)
  • Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. (cdc.gov)
  • For 14 cell types, we identified 26,597 independent cis-expression quantitative trait loci (eQTLs), and 62,305 trans-eQTL, with the majority showing cell type specific effects on gene expression. (iscb.org)
  • Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
  • An intriguing aspect to the association of these genes with retinal disease is that splicing occurs in every cell of the body so the genes must have a general housekeeping function, yet the disease pathology is restricted to the rod photoreceptors of the retina. (molvis.org)
  • immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
  • These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)
  • Using an oligonucleotide-based microarray, we normalized the transcriptomic data by genomic DNA signals in order to have access to inter-gene comparison data. (biomedcentral.com)
  • We reveal that widely used splice-aware aligners, such as STAR and HISAT2, can introduce erroneous spliced alignments between repeated sequences, leading to the inclusion of falsely spliced transcripts in RNA-seq experiments. (bvsalud.org)
  • In a normal lymph node that displays both distinct gene expression patterns and histological landmarks, we observed a neutral iCNV profile. (kth.se)
  • As one of the two distinct complexes, namely, Polycomb Repressive Complex 1 (PRC1) and PRC2, PRC2 mediates gene silencing by modulating chromatin structure [ 2 ]. (hindawi.com)
  • The genome of P. falciparum contains hundreds of genes that show CVGE, such that individual parasites within an isogenic population express these genes at very different levels, often fully active or completely silenced [15] . (plos.org)
  • ncRNAs play important roles in controlling gene expression at both transcriptional and post-transcriptional levels. (ijbs.com)
  • In Paper I , we describe a protocol for transcript profiling of single cells, capable of measuring the relative expression levels for genes of interest. (kth.se)
  • Conserved motif and gene structure analysis revealed high levels of conservation within and between phylogenetic subfamilies. (figshare.com)
  • The effect of Buchnera genome evolution on gene expression levels has also been analysed in order to assess the constraints imposed by the obligate symbiosis with aphids, underlining the importance of some gene sets for the survival of the two partners. (biomedcentral.com)
  • This is a perfect illustration of the mutual influence between gene expression levels and chromosomal organization in bacteria. (biomedcentral.com)
  • Cells explanted from the tumors and grown in culture also show no expression of 9E3, in marked contrast to the very high level found in similarly cultured RSV-transformed CEFs. (silverchair.com)
  • However, the expression of the gene in the periphery of tumors leaves open the possibility that 9E3 may still be involved in RSV carcinogenesis. (silverchair.com)
  • We found a decrease in cytoskeleton-related protein expression, a known hallmark of prostate tumors. (stanford.edu)
  • Multivariate Cox regression analysis demonstrated no independent prognostic significance of stromal Annexin A2 expression. (cancerindex.org)