• In vitro studies of adipocyte differentiation indicate that Foxo1 expression is induced in the early stages of adipogenesis in 3T3-F442A cells. (medscape.com)
  • Interestingly, Foxo1 phosphorylation appears to be tightly regulated [ 57 ] during differentiation of 3T3-F442A cells. (medscape.com)
  • Expression of a phosphorylation-defective Foxo1 prevents adipocyte differentiation by altering expression patterns of genes involved in cell cycle control and adipogenesis. (medscape.com)
  • [ 57 ] The emerging model of Foxo1 function in fat cells is that it may control the coupling of extracellular (hormonal) cues that activate adipocyte differentiation with the cell cycle machinery. (medscape.com)
  • 9. Growth factor-mediated proliferation and differentiation of insulin-producing INS-1 and RINm5F cells: identification of betacellulin as a novel beta-cell mitogen. (nih.gov)
  • 11. Regulation of preadipocyte factor-1 gene expression during 3T3-L1 cell differentiation. (nih.gov)
  • When 3T3-L1 cells in which smad3 had been knocked down using an shRNA were treated using a white adipocyte differentiation medium, they expressed elevated mRNA and protein expression of BAT-specific markers. (genengnews.com)
  • The differentiation of a preadipocyte into a mature adipocyte is a highly regulated process that requires a scripted program of transcriptional events leading to changes in gene expression. (aspetjournals.org)
  • Exposure of 3T3-L1 cells to INT-747 (6-ethyl cheno-deoxycholic acid), a potent and selective FXR ligand, increases preadipocyte differentiation induced by a differentiating mixture containing insulin. (aspetjournals.org)
  • Augmentation of differentiating mixture-induced differentiation of 3T3-L1 cells by INT-747 associated with induction of aP2, C/EBPα, and PPARγ2 mRNAs along with other adipocyte-related genes. (aspetjournals.org)
  • Since Adipocyte Determination and Differentiation factor 1, ADD1, has been postulated as a transcription factor in the regulation of adipocyte genes, we investigated the role of ADD1 in All regulation of the FAS gene. (tennessee.edu)
  • It is commonly used in cell biology research, particularly in studies of cell growth, differentiation, and transformation. (altogen.com)
  • In summary, BALB/3T3 cells are a valuable cell line in biomedical research due to their utility in studying cell growth, differentiation, and transformation, particularly in the context of cancer biology. (altogen.com)
  • Cell-biology-related research on preadipocyte (adiposal-mesenchymal cell) differentiation laid the foundation for discoveries of adipose-derived cells (both in stromal vascular fraction, or SVF, and adipose-derived stromal/stem cells, or ASCs), which had become an increasing interest to both stem cell biologists and clinicians because of their potential for angiogenesis and suppression of inflammation for tissue engineering and treatments. (scirp.org)
  • In addition, adipogenesis of murine 3T3-L1 and human Simpson-Golabi-Behmel Syndrome preadipocytes was dose- and time-dependently reduced by PM, as demonstrated by Oil Red O staining and reduced expression of adipogenic differentiation genes. (nih.gov)
  • Signal transduction plays an important role in activating cellular functions, cell differentiation , and cell proliferation . (lookformedical.com)
  • For example: in a murine model, Crohn's disease can be precipitated by a norovirus, but only when both a specific gene variant is present and a certain toxin has damaged the gut. (wikipedia.org)
  • To address this issue, we used murine 3T3-L1 as a cell model and the fatty acid synthase (FAS) gene as a lipogenic marker. (tennessee.edu)
  • Determined by its ability to stimulate the proliferation of murine NIH-3T3 cells. (peprotech.com)
  • Intracellular distributions of the putative cytosolic pH indicator dyes BCECF [2′,7′-bis-(2-carboxyethyl)-5(and 6)-carboxyfluorescein], C.SNARF [5(and 6)-carboxy-seminaphthorhodafluor-1], and C.SNARF-calcein have been examined in Neurospora crassa and in murine fibroblasts (NIH-3T3 cells) under conditions in which both kinds of cells produce visible microscopic vacuoles. (biologists.com)
  • MISSION ® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines. (sigmaaldrich.com)
  • Although there was no difference in calorific intake between the wild-type mice and those lacking the Smad3 gene, the engineered animals displayed significantly reduced fat mass and smaller white adipocytes. (genengnews.com)
  • Mouse embryonic fibroblasts (MEFs) from these mice were impaired in their ability to differentiate into white adipocytes, and both MEFs and WAT showed reduced expression of white adipocyte-specific genes. (genengnews.com)
  • In order to identify All regulatory sequences in the FAS promoter, we transfected fusion constructs linking various deletions of the FAS promoter to the luciferase reporter gene into 3T3-L1 adipocytes. (tennessee.edu)
  • We demonstrated that overexpression of a dominant negative form of ADD1 in 3T3-L1 adipocytes markedly attenuates FAS induction by All. (tennessee.edu)
  • In conclusion, our data indicate that All exerts insulin like effects on the FAS gene by targeting similar regulatory sequences and transcription factors as insulin and suggest that paracrine effects of All in adipocytes contribute to adipocyte hypertrophy. (tennessee.edu)
  • Prolonged treatment of 3T3-L1 adipocytes decreases expression of GLUT4, the insulin-responsive glucose transporter. (johnshopkins.edu)
  • Nuclear extract from 3T3-L1 adipocytes protected the region from -707 to -681 in the GLUT4 5'-flanking region from DNase I digestion. (johnshopkins.edu)
  • A reporter gene construct in which bases -706 to -676 were deleted was not repressed by insulin treatment, confirming that this sequence is necessary for the repression of the GLUT4 promoter by insulin in 3T3-L1 adipocytes. (johnshopkins.edu)
  • Treatment with general control nonderepressible 2 inhibitor (GCN2ib) alleviates adipocyte-mediated translational repression and rescues ALL cell quiescence thereby significantly reducing the cytoprotective effect of adipocytes against chemotherapy and other extrinsic stressors. (nature.com)
  • The cell biology of adipocytes has led to a number of promising concepts in the last century, especially in the areas of obesity and associated diseases, lipid homeostasis and endocrine functions. (scirp.org)
  • Recent advances in adipogenesis had provided insights into understanding of the complex cues for influencing the cytoarchitecture, epigenomic remodeling, signaling pathways and transcription regulators on gene actions for both white and brown adipogenic progression from mesenchymal stem cells to matured committed adipocytes. (scirp.org)
  • RESULTS: Hesperetin affected the viability of 3T3-L1 adipocytes with IC50 of 447.4 µM, 339.2 µM and 258.8 µM (24 h, 48 and 72 h, respectively). (bvsalud.org)
  • Hesperetin significantly reduced the gene and protein expression of both collagen VI and osteopontin in 3T3-L1 pre-adipocytes, in a time- and dose-dependent manner. (bvsalud.org)
  • In the presence of pro-inflammatory stimuli, Lobe efficiently suppressed expressions of pro-inflammatory genes in macrophages and adipocytes. (inforang.com)
  • Human obesity reflects an imbalance between energy expenditure and caloric intake resulting from an increase in either the number or the size of fat cells, or both, and is induced by the enlargement of adipocytes as well as the generation of new adipocytes from precursor cells [ 2 ]. (ijbs.com)
  • Studies have been performed to determine if cadmium chloride (CdCl(2)) can induce morphological cell transformation, DNA from CdCl(2)-induced transformed cells can transform other mammalian cells, and the transformed cells induced by CdCl(2) can form tumors in nude mice. (cdc.gov)
  • Non-transformed BALB/c-3T3 cells and cells from 10 transformed cell lines induced by CdCl(2) were injected into both axillary regions of nude mice. (cdc.gov)
  • All 10 CdCl(2)-induced transformed cell lines formed fibrosarcomas in nude mice within 39 days of inoculation. (cdc.gov)
  • Within this time period, no tumors were found in nude mice injected with non-transformed BALB/c-3T3 cells. (cdc.gov)
  • 17. Overexpression of Pref-1 in pancreatic islet β-cells in mice causes hyperinsulinemia with increased islet mass and insulin secretion. (nih.gov)
  • Previous research has also found that TGF-β levels correlate with obesity in mice and humans, while studies by Dr. Rane's team has identified an important role for the TGF-β/Smad3 pathway in the regulation of insulin gene transcription and β cell function. (genengnews.com)
  • They found that mice in which the Smad3 gene was globally knocked out exhibited enhanced insulin sensitivity and increased whole-body glucose uptake, which was particularly marked in WAT compared with other metabolically active tissues such as skeletal muscle. (genengnews.com)
  • Our results show that FXR is expressed in the white adipose tissue of adult mice and in differentiated 3T3-L1 cells but not in undifferentiated preadipocytes. (aspetjournals.org)
  • Regulation of adipocyte-related genes by INT-747 was lost in FXR-/- mice, indicating that modulation of these genes by INT-747 requires an intact FXR. (aspetjournals.org)
  • Compared to other two TZDs, Lobe enhanced beige adipocyte formation and thermogenic gene expression in inguinal white adipose tissue (IAT) of lean mice, which would be attributable to cold-induced thermogenesis. (inforang.com)
  • 13. Effect of homologous placental lactogens, prolactins, and growth hormones on islet B-cell division and insulin secretion in rat, mouse, and human islets: implication for placental lactogen regulation of islet function during pregnancy. (nih.gov)
  • 18. Regulation of the diabetes-associated autoantigen IA-2 in INS-1 pancreatic beta-cells. (nih.gov)
  • Nevertheless, the regulation of Lin28 gene expression has not been thoroughly studied. (nih.gov)
  • The origin of these cells and their regulation at the genetic level is unclear, however. (genengnews.com)
  • Differential methylation is found within genes associated with obesity, epigenetic regulation and development, such as CETP , FOXP2 , HDAC4 , DNMT3B , KCNQ1 and HOX clusters. (biomedcentral.com)
  • these regions were located in or near genes previously implicated in body weight regulation or diabetes. (biomedcentral.com)
  • This sequence does not show homology to previously described insulin response elements and thus represents a distinct mechanism of gene regulation by insulin. (johnshopkins.edu)
  • BALB/3T3 cells are often used as a model system for studying cell cycle regulation and the molecular mechanisms of cell transformation. (altogen.com)
  • Results: In the present study, hundreds of differentially expressed genes (DEGs) were selected in LM-CRC compared to primary CRC, and these DEGs were significantly associated with the regulation of endopeptidase activity, blood coagulation, and metabolic processes. (bvsalud.org)
  • Analysis with the RAS effector-loop mutants that interact specifically with Raf, Ral GDS pathways highlighted the importance of the RalGDS pathways in the regulation of S100A10 gene expression. (port.ac.uk)
  • The 3T3 cell line has become the standard fibroblast cell line. (signagen.com)
  • The system contains an engineered 3T3 mouse fibroblast cell line, which expresses the firefly luciferase reporter gene under the control of Wnt-responsive promoters (TCF/LEF). (enzolifesciences.com)
  • Altogen Biosystems synthesizes biodegradable polymer-based high-efficiency transfection reagent kits for the BALB/3T3 mouse embryo fibroblast cell line. (altogen.com)
  • 17, 18] TCF7L2 protein is increased during adipogenesis in 3T3-L1 cells and in primary adipocyte stem cells. (medscape.com)
  • We hypothesized that exposing preadipocytes to TBBPA could influence adipogenesis via genes other than those in the PPARγ pathway due to its structural similarity to bisphenol A, which demonstrates varied endocrine disrupting activities. (nih.gov)
  • Mouse-derived 3T3-L1 preadipocytes were induced to differentiate and continually treated with TBBPA for 8 days. (nih.gov)
  • BALB/3T3 cells are adherent and grow in vitro as monolayer cultures. (altogen.com)
  • BALB/3T3 cells are particularly useful for investigating the effects of oncogenes and other genetic alterations on cell growth and transformation. (altogen.com)
  • BALB/3T3 cells have also been used to develop assays for screening potential cancer therapeutics. (altogen.com)
  • siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into BALB 3T3 cells following the recommended protocol. (altogen.com)
  • 5. The stimulatory effect of growth hormone, prolactin, and placental lactogen on beta-cell proliferation is not mediated by insulin-like growth factor-I. (nih.gov)
  • 6. Growth hormone- and prolactin-induced proliferation of insulinoma cells, INS-1, depends on activation of STAT5 (signal transducer and activator of transcription 5). (nih.gov)
  • 12. Role of prolactin versus growth hormone on islet B-cell proliferation in vitro: implications for pregnancy. (nih.gov)
  • Functionally, adipocyte niches elicit a fate switch in ALL cells towards slow-proliferation and cellular quiescence, highlighting the critical contribution of the adipocyte dynamic to disease establishment and chemotherapy resistance. (nature.com)
  • Dekanty, A. Differential involvement of ERK1-2 and p38MAPK activation on Swiss 3T3 cell proliferation induced by prostaglandin F2α. (uba.ar)
  • Interestingly, RSPO1 -mutated PPK skin expressed markers of increased proliferation, dedifferentiation and altered cell-cell adhesion. (biomedcentral.com)
  • It consists of persistent thickening of the epidermis over the palms and soles and is characterized by keratinocyte hyperproliferation and excessive production of horny layer (hyperkeratosis) that develop in response to an inherent fragility of the tissue or a dysregulation of cell proliferation. (biomedcentral.com)
  • 1. Expression, biosynthesis and release of preadipocyte factor-1/ delta-like protein/fetal antigen-1 in pancreatic beta-cells: possible physiological implications. (nih.gov)
  • Several genes are associated with adipogenesis, including the CAAT/enhancer-binding protein (C/EBPs) and peroxisome proliferator-activated receptor (PPAR) families of transcription factors. (aspetjournals.org)
  • Protein localization in cells has been analyzed by fluorescent labeling using indirect immunofluorescence and fluorescent protein tagging. (frontiersin.org)
  • Lamellipodia are one of the actin-dependent subcellular structures involved in cell migration and are mainly generated by the Wiskott-Aldrich syndrome protein (WASP)-family verprolin homologous protein 2 (WAVE2) and the membrane remodeling I-BAR domain protein IRSp53. (frontiersin.org)
  • Focal adhesion is another actin-based structure that contains vinculin protein and promotes lamellipodia formation and cell migration. (frontiersin.org)
  • The luciferase activity from the reporter gene in this cell line can be up-regulated in a dose-dependent manner upon the addition of exogenous Wnt protein/Wnt agonist or down-regulated by a further addition of a Wnt antagonist to the cell culture medium. (enzolifesciences.com)
  • The LEADING LIGHT ® Wnt Cell Line Assay Reagents contains Luciferase substrate and assay buffer, Lithium Chloride, HEPES, Wnt3a protein (positive control), Dkk-1 protein, and a 96-well black plate. (enzolifesciences.com)
  • Wnt reporter cells were treated with indicated doses of Wnt3a protein. (enzolifesciences.com)
  • Mechanistically, adipocyte niche interaction targets posttranscriptional networks and suppresses protein biosynthesis in ALL cells. (nature.com)
  • Goin, M., Pignataro, O., Jimenez de Asua, L., Early cell cycle diacylglycerol (DAG) content and protein kinase C (PKC) activity enhancement potentiates prostaglandin F2 alpha (PGF2 alpha) induced mitogenesis in Swiss 3T3 cells (1993) FEBS Lett. (uba.ar)
  • In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. (thermofisher.com)
  • Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes. (lookformedical.com)
  • Oncogenic RAS expression increased S100A10 gene expression which resulted in an increase in S100A10 protein levels. (port.ac.uk)
  • Quantitative PCR revealed that TBBPA exposure increased early expression of genes involved in glucocorticoid receptor (GR) signaling and PPARγ transcriptional activation, as well as upregulating downstream genes needed for adipocyte maintenance and nontraditional ER signaling, such as Gpr30. (nih.gov)
  • We demonstrated that All up-regulates the FAS gene at the transcriptional level. (tennessee.edu)
  • REST regulates distinct transcriptional networks in embryonic and neural stem cells. (ox.ac.uk)
  • The maintenance of pluripotency and specification of cellular lineages during embryonic development are controlled by transcriptional regulatory networks, which coordinate specific sets of genes through both activation and repression. (ox.ac.uk)
  • The transcriptional repressor RE1-silencing transcription factor (REST) plays important but distinct regulatory roles in embryonic (ESC) and neural (NSC) stem cells. (ox.ac.uk)
  • We studied the molecular mechanisms responsible for differential expression of PGC-1alpha in HIB1B (BAT) and 3T3-L1 white adipose tissue (WAT) precursor cell lines. (elsevierpure.com)
  • BACKGROUND: In stem cell applications, apart from bone marrow and adipose tissue, compact bone is also used as an alternative. (bvsalud.org)
  • The high expression of proinflammatory genes in visceral adipose tissue of the HFD group was significantly attenuated by PM. Treatment with PM partially prevented HFD-induced mild vascular dysfunction. (nih.gov)
  • This effect was reversed by guggulsterone, an FXR antagonist, and partially reverted by GW9662 (2-chloro-5-nitro- N -phenylbenzamide), a selective PPARγ antagonist, indicating that FXR modulates adipocyte-related genes by PPARγ-dependent and -independent pathways. (aspetjournals.org)
  • Secreted R-spondin (RSPO) proteins play a key role in reproductive organ development, epithelial stem cell renewal and cancer induction by reinforcing canonical Wnt signaling. (biomedcentral.com)
  • Cultured PPK patient's keratinocytes exhibited increased expression of cell‒matrix adhesion proteins and extracellular matrix remodeling enzymes. (biomedcentral.com)
  • Indeed, proteins affected in hereditary PPK fulfill various functions, including mechanical stabilization (e.g. defects of keratins or desmosomal proteins), cell signaling and cell-to-cell communication (e.g. alterations of signaling, transport, receptors). (biomedcentral.com)
  • Cells were co-stained with Hoechst (nuclei) and Nile red (lipid). (nih.gov)
  • The filtered nuclei were then transferred to a new 15ml tube (Falcon) and pelleted by centrifuge at 500xg for 5min and washed once with 1ml cell lysis buffer. (nih.gov)
  • After fixation, the nuclei were washed twice in 1ml nuclei wash buffer (cell lysis buffer without IGEPAL), and re-suspended in 500ul nuclei wash buffer. (nih.gov)
  • The nuclei were then washed once with nuclei wash buffer and filtered through 1ml Flowmi cell strainer (Flowmi). (nih.gov)
  • Nuclei were washed once with nuclei wash buffer and filtered with 1ml Flowmi cell strainer (Flowmi) twice, counted and redistributed into eight 96-well plates with each well including 2,500 nuclei in 5µL nuclei wash buffer and 5µL elution buffer (Qiagen). (nih.gov)
  • DNAs from five CdCl(2)-induced transformed cell lines were isolated and gene transfection assay was performed using NIH-3T3 cells. (cdc.gov)
  • The LEADING LIGHT ® Wnt Cell Line Assay Reagents are intended for use with the LEADING LIGHT ® Wnt Reporter Cell Line, which is the basis for a cell-based luciferase activity test ENZ-61001 LEADING LIGHT® Wnt Reporter Assay Starter Kit suitable for a 96-well plate format. (enzolifesciences.com)
  • Cells were treated with a range of hesperetin concentrations, and the cell viability was determined using MTT assay. (bvsalud.org)
  • GenJet In Vitro DNA Transfection Reagent for 3T3 Cells [SL100489-3T3] - $166.00 : SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents. (signagen.com)
  • GenJet DNA In Vitro Transfection Reagent for NIH3T3 Cells is pre-optimized for NIH3T3 cell transfection. (signagen.com)
  • HiPerFect Transfection Reagent is a unique blend of cationic and neutral lipids that enables effective siRNA uptake and efficient release of siRNA inside cells, resulting in high gene knockdown even when using low siRNA concentrations. (qiagen.com)
  • Cell-type-specific protocols using HiPerFect Transfection Reagent are available at the TransFect Protocol Database. (qiagen.com)
  • A range of suspension cell lines and differentiated and undifferentiated macrophages have been tested using HiPerFect Reagent for siRNA transfection. (qiagen.com)
  • Transfection with HiPerFect Reagent is a superior alternative to electroporation for many of these cell types. (qiagen.com)
  • Some cell lines were not amenable to transfection using HiPerFect Reagent (see Table 2). (qiagen.com)
  • 3. Effects of sex and pregnancy hormones on growth hormone and prolactin receptor gene expression in insulin-producing cells. (nih.gov)
  • 15. ACTH stimulates insulin secretion from MIN6 cells and primary mouse and human islets of Langerhans. (nih.gov)
  • 16] Potential mechanisms through which TCF7L2 variants influence T2DM include its role in adipogenesis, myogenesis, and pancreatic islet development, as well as in beta-cell survival and insulin secretory granule function. (medscape.com)
  • Finally, TCF7L2 polymorphisms have been associated with impaired insulin secretion, glucose production, and glucose tolerance via direct effects on pancreatic islet beta cells. (medscape.com)
  • In addition, INT-747 enhances both insulin-induced serine phosphorylation of Akt and glucose uptake by 3T3-L1 cells. (aspetjournals.org)
  • Furthermore, we demonstrated that a mutation in the insulin response element abolishes not only insulin but also All responsiveness of the FAS gene. (tennessee.edu)
  • Cooke, DW & Lane, MD 1998, ' A sequence element in the GLUT4 gene that mediates repression by insulin ', Journal of Biological Chemistry , vol. 273, no. 11, pp. 6210-6217. (johnshopkins.edu)
  • In this study, we have investigated the role of the farnesoid X receptor (FXR), a bile acid-activated nuclear receptor, in regulating adipogenesis in a preadipocyte cell line (3T3-L1 cells). (aspetjournals.org)
  • 2 Department of Developmental and Cell Biology, University of California, Irvine, California 92697. (nih.gov)
  • Connective tissue comprises fibroblasts, active cells that help maintain tissue metabolism. (altogen.com)
  • Mouse embryonic fibroblasts nourish embryonic stem cells by secreting growth factors in addition to providing a cellular matrix to promote cell growth. (altogen.com)
  • Oncogene activation and loss of tumor suppressor genes are known to play a role in tumor initiation as well as its progression. (nih.gov)
  • Using an orthotopic mouse model of pleural mesothelioma, we determined that relatively high doses of both CD28- and 4-1BB-based second-generation CAR T cells achieved tumor eradication. (nih.gov)
  • however, PD-1 upregulation within the tumor microenvironment inhibited T cell function. (nih.gov)
  • Western Blot: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin expression in various small-round cell tumor lines. (novusbio.com)
  • The single-cell RNA sequencing (scRNA-seq) datasets of CRC were collected from and analyzed in the Tumor Immune Single-cell Hub (TISCH) database. (bvsalud.org)
  • DNA from all five CdCl(2)-induced transformed cell lines tested was found to induce varying degrees of transfection-mediated transformation in NIH-3T3 cells. (cdc.gov)
  • These results indicate that CdCl(2) is capable of inducing morphological cell transformation and that the transformed cells induced by CdCl(2) are potentially tumorigenic. (cdc.gov)
  • b) and cell transformation in vitro (Keshava et al. (nih.gov)
  • It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES . (lookformedical.com)
  • We have observed that transformation of cancer cells with oncogenic forms of RAS increases plasmin proteolytic activity by 2- to 4-fold concomitant with a 3-fold increase in cell invasion. (port.ac.uk)
  • In electrophoretic mobility shift assays, the transcription factors CREB and C/EBPß, but not C/EBPa and C/EBPd, bound to the CRE on the PGC-1alpha promoter region in HIB1B and 3T3-L1 cells. (elsevierpure.com)
  • Refer to the following optimal transfection conditions for maximal transfection efficiency on NIH 3T3 cells. (signagen.com)
  • The use of a static magnetic field has been shown to result in dramatic increase in transfection efficiency of gene delivery when compared with the conventional transfection system [ 9 , 10 ]. (hindawi.com)
  • Previous studies have revealed that chitosan, like other cationic polymers, displayed concentration-dependent toxicity toward cells in vitro, although it had many advantages as a gene vector [ 6 ]. (hindawi.com)
  • We used both of the advantages of magnetic nanoparticles and biodegradable polymers, and the application of the novel polymer-Fe 3 O 4 complexes as gene vectors in vitro was then described at length. (hindawi.com)
  • The burgeoning preclinical and clinical experience with human adult adipose-derived cells appears to be promising but interpretation of the current literature with in vitro studies, translational research and FDA-registered investigations emphasizes the need for standardized methods to advance basic science knowledge and beneficial safe clinical outcomes. (scirp.org)
  • Finally, our in vitro experiments proved that ANGPTL2+CAFs and SPP1+macrophages promote the metastasis of CRC cells. (bvsalud.org)
  • Cells propagated in vitro in special media conducive to their growth. (lookformedical.com)
  • In fact, Foxo1 haploinsufficiency has been shown to decrease adipocyte size and increase expression of genes that promote lipid metabolism. (medscape.com)
  • Their findings are reported in Cell Metabolism in a paper titled "Protection from Obesity and Diabetes by Blockade of TGF-β/Smad3 Signaling. (genengnews.com)
  • These results provide the first in vivo evidence supporting essential regulatory roles for KLFs in fat metabolism in C. elegans and shed light on the human counterpart in disease-gene association. (ijbs.com)
  • Todaro and Green originally obtained their 3T3 cells from Swiss mouse embryo tissue. (signagen.com)
  • For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of theendogenous myogenin gene as well as transactivation of MyoD. (novusbio.com)
  • Jimenez de Asua, L., Richmond, K.M., Otto, A.M., Two growth factors and two hormones regulate initiation of DNA synthesis in cultured mouse cells through different pathways of events (1981) Proc. (uba.ar)
  • The promoter region of Lin28 gene also became more sensitive to nuclease digestion after TSA treatment, suggesting that Lin28 expression was mediated by the relaxation of chromatin. (nih.gov)
  • In agreement with these findings, we observed a significant enrichment of H3K9ac and reduced occupancy of H3K9me2 at the Lin28 promoter region in TSA-treated NIH/3T3 cells and in undifferentiated P19 embryonal carcinoma cells. (nih.gov)
  • The SMA disease gene, termed Survival of Motor Neurons (SMN), is deleted or mutated in over 98% of SMA patients. (nih.gov)
  • Bisulfite-sequencing analysis did not reveal a correlation between its promoter DNA methylation and expression of Lin28 gene. (nih.gov)
  • A greater proportion of CpGs are hypermethylated before weight loss and increased methylation is observed in the 3′ untranslated region and gene bodies relative to promoter regions. (biomedcentral.com)
  • Genes investigated with differential promoter methylation all show significantly different levels of mRNA before and after gastric bypass. (biomedcentral.com)
  • Animal and human studies have shown that the DNA methylation status of genes in offspring can be altered in utero by the maternal dietary environment [ 19 - 23 ]. (biomedcentral.com)
  • Chromatin immunoprecipitation studies demonstrated that C/EBPß and CREB bound to the CRE region in HIB1B and 3T3-L1 cell lysates. (elsevierpure.com)
  • Embryonic stem cells are a widely-used means for cell and molecular biology research, and mouse fibroblast cell lines are imperative for the proper growth of stem cells. (altogen.com)
  • Each mouse embryo was minced into small pieces by blade in 1 mL ice-cold cell lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630 from77, modified to also include 1% SUPERase In and 1% BSA) and transferred to the top of a 40um cell strainer (Falcon). (nih.gov)
  • BALB/3T3 is a cell line that was derived from a mouse embryo. (altogen.com)
  • MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. (novusbio.com)
  • Studies relating to Wnt signaling have advanced research in molecular embryology, stem cell biology, tumorigenesis, regenerative medicine, and rational drug discovery. (enzolifesciences.com)
  • comprising a highly diverse network of cellular (mesenchymal stroma, osteoblasts and endothelial cells), soluble and structural factors that work together to coordinate and maintain haematopoietic stem cell (HSC) function 11 . (nature.com)
  • Stem cell therapy is the use of stem cells for treatment or prevention of different diseases, including stroke, spinal cord injury, Alzheimer's disease, Parkinson's disease and other neurological conditions. (altogen.com)
  • This cell line could be useful for studying embryonic tissue of mammals, such as stem cells and as a transfection host in a variety of cell biology research. (altogen.com)
  • Since the specialty of Plastic Surgery with its position statement on stem cells and fat grafting is committed to advancing evidence-base preclinical and clinical studies in compliance with FDA-regulations, an updated review of cell biology provides insights to achieve these goals. (scirp.org)
  • In our study, we investigated the effect of stem cell derived from compact bone on rat zygomatic arch defect. (bvsalud.org)
  • To better understand the relationship between expression of an oncogene and genetic instability, we have studied a cell line expressing an activated human Ha-ras under the control of bacterial lactose operon regulatory elements for changes in methotrexate resistance and dihydrofolate reductase (dhfr) gene amplification following mutant Ha-ras induction. (nih.gov)
  • Immunofluorescence analysis of Phospho-MDM2 (Ser395) was performed using 70% confluent log phase HeLa cells treated with 0.5uM Doxorubicin for 24 hours. (thermofisher.com)
  • Immunofluorescence analysis of methanol-fixed HeLa cells using Cortactin (PA5-29799) antibody at 1:200 dilution. (labome.cn)
  • Lane 5: human Hela whole cell lysate. (labome.cn)
  • NIH 3T3 cells come from a cell line established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. (signagen.com)
  • The researchers confirmed these findings in cultured 3T3-L1 cells, an MEF adipose like cell line that can be triggered to differentiate into adipocyte-like cells. (genengnews.com)
  • The BALB cell line (also known as 3T3 clone A31) was established in 1968 from fibroblast tissues found in BALB/c mouse (Mus musculus) embryos. (altogen.com)
  • Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. (sigmaaldrich.com)
  • C/EBPß expression was induced by cAMP only in HIB1B cells, and overexpression of C/EBPß rescued cAMP-inducible PGC-1alpha and UCP1 expression in 3T3-L1 cells. (elsevierpure.com)
  • Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. (lookformedical.com)
  • Our results identify that Lin28 expression in mouse cells is epigenetically regulated by histone modification. (nih.gov)
  • The set represents ~36,000 full length genes and EST clusters derived from sequence clusters in Build 74 of the Mouse Unigene Database. (nih.gov)
  • The expression of Lin28 gene predominantly occurs in pluripotent cells and cancer cells. (nih.gov)
  • SignaGen Laboratories, A Gene Delivery Company Providing Custom AAV Adenovirus Lentivirus Production Services & Manufacturing DNA/siRNA Transfection Reagents. (signagen.com)
  • Transfection of siRNA can result in off-target effects, in which siRNAs affect the expression of non-homologous or partially homologous gene targets. (qiagen.com)
  • Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. (novusbio.com)
  • The cells in addition displayed increased mRNA expression of markers of brown adipogenesis. (genengnews.com)