Generation of transgenic mice microinjections. Medical search. Frequent questions

Researchers can take advantage of three common methods of producing transgenic animals for their research. (connectusfund.org)
There are three ways of producing transgenic animals, one is called DNA microinjection. (sd43.bc.ca)

Microinjection of Alt-R ® CRISPR/Cas9 RNP system is particularly valuable for generation of knockout and transgenic mice. (genengnews.com)
CRISPR/Cas9-mediated NHEJ in mouse embryos will be used to generate founder animals harboring insertion/deletion (indel) or interval deletion knockout alleles. (bcm.edu)
CRISPR/Cas9 method for the production of genome-edited mouse line. (infrafrontier.gr)
Established in response to a need for in-house mouse services, the LUTCF provides expertise in cryopreservation of embryos by IVF or natural matings, sperm cryopreservation, rederivation services, strain expansion by IVF, ES morula/blastocyst injections, pronuclear DNA microinjections, and injection of CRISPR edited DNA. (lu.se)
This course will provide training in genome editing and cell engineering in mammalian cells and mouse embryos using the highly efficient CRISPR/Cas9 system. (sb-3.org)
Participants will learn design of CRISPR targets using bioinformatics tools, generation of gene knock-outs/knock-ins, and target validation using the most current technologies. (sb-3.org)
This course is aimed at researchers who are familiar with basic molecular and cell biology techniques and who want to learn how to create an engineered mammalian cell line or mouse model using the most recent and advanced CRISPR/Cas9 system. (sb-3.org)
By combining high throughput electroporation with gene knockout strategies generated by AlphaKnockout , our proprietary AI (artificial intelligence) gene targeting expert system, and special ovulation induction technologies, we successfully established and cryopreserved 100 strains of CRISPR-AI knockout mouse sperms. (cyagen.com)
Encouraged by the success of the first 100 cases, we decided to create the CRISPR-AI knockout mouse sperm bank with the goal to cryopreserve strains covering most mouse genes. (cyagen.com)

at the 1986 ISIR-TNO Annual Meeting, September 7-12, 1986, reported the production of transgenic mice carrying mouse interferon beta or gamma genes coupled to the mouse MT-I or heat shock promoters. (justia.com)
Tweezertrodes have proven particular useful for embryonic or even spermatogonial cell electroporation for effective production of transgenic and knockout mice. (btxonline.com)

The double transgenic mice (dTg) were obtained by mating: (i) transgenic mice expressing the hemagglutinin of influenza virus under the insulin promoter with (ii) transgenic mice expressing specific T lymphocytes with receptor for the immunodominant epitope of the same virus. (promisekit.org)
Transgenic frogs that express the enzyme in the germline can be bred with animals harboring a transposon substrate to generate double transgenic lines where remobilization will occur in the germline in subsequent generations. (biomedcentral.com)

Transgenic animals are creatures who have had their genome altered because of the transfer of a gene or genes from another breed or species. (connectusfund.org)
Transgenic animals make it possible for scientists to study the functioning of particular genes at the level of the entire organism. (connectusfund.org)
Transgenic animals have changed the world by having the ability to have genes from one animal to another animal to in the case of cows produce more milk and in other cases helping advances medical studies and sciences and in many other different fields of study. (sd43.bc.ca)
But it was only seven years later that researchers could edit genes that were passed on to future generations. (mongabay.com)
How are genes inserted into mice? (promisekit.org)
Technologies used to generate transgenic animals overexpressing specific transgenes or mutant models with specific endogenous genes modified (knockout and knockin animal models). (uab.cat)
Transgenic organisms are able to express foreign genes because the genetic code is similar for all organisms. (meddic.jp)
Gordon and Ruddle, Science, 214:1244 (1981) claimed integration and stable germline transmission of genes injected into mouse pronuclei. (justia.com)
Genetically altered (GA) mice are used extensively to study the function and regulation of genes and their role in human development and disease. (nc3rs.org.uk)
1939 International Committee on Standardized Nomenclature for Mice begins, bringing order to the naming of mice and their genes. (jax.org)

If the guide RNAs were (i) tested in mouse embryos by the core and the core approved their use based on the genotyping results and (ii) the core conducted the founder genotyping, the core can review results, redesign the approach and reagents if needed, and the electroporation can be repeated. (bcm.edu)
If the guide RNAs were not tested in mouse embryos or the investigator conducts their own genotyping, there will be no reduced costs for additional work. (bcm.edu)
This is currently the most popular method for the generation of targeted knock-out and knock-in models by ES-cell injections into 8-cell morulae and/or blastocysts of mouse embryos. (lu.se)
These practicals will address injection of nucleic acids into the pronucleus and cytoplasm of fertilised mouse oocytes, embryonic stem cell transfer into blastocysts, piezo-assisted stem cell transfer into 8-cell stage embryos and microscopy training. (embl.org)
Microinjection into mouse zygotes will be covered, as well as cell transfer into embryos and blastocysts. (embl.org)
The genome editing efficiency of up to two guide RNAs selected by the core as part of a project design or two guide RNAs selected by an investigator and approved by the core will be tested in mouse embryos. (bcm.edu)
The first method involves DNA delivery by retroviral infection of mouse embryos at different developing stages. (promisekit.org)
Intact/injected embryos, pregnancy and birth rate, survival of pups 7 days after birth, number of transgenic pups and overall transgenic efficiency was registered and analyzed by Z test of proportions for each group. (uchile.cl)
Building on our proprietary Noncontrolled-Rate Protein-Free Cryopreservation Media, we have developed optimized cryopreservation techniques for mouse sperms and embryos which have greatly improved stability and increased cryorecovery and birth rates. (cyagen.com)
The team at University of Leeds led by Dr Virginia Pensabene has developed a novel and reliable microfluidic device that improves the developmental competence of in vitro -derived mouse embryos to allow the use of non-surgical embryo transfer (NSET) in the generation of transgenic mice. (nc3rs.org.uk)
The team at University of Leeds led by Dr Virginia Pensabene has developed a novel and reliable microfluidic device that improves the developmental competence of in vitro -derived mouse embryos and their implantation potential, enabling the use of non-surgical embryo transfer (NSET) in the generation of transgenic mice. (nc3rs.org.uk)
Effects of Uterine Cells-Conditioned Media on Expression of DNMT3B and DNMT3C in Mouse Embryos Cultured in a Microfluidic Device. (nc3rs.org.uk)

The strain is now valued as a source of embryonic stem cells for making knockout mice. (jax.org)

Investigators can opt to have the guide RNAs tested for genome editing efficiency in mouse zygotes prior to the production attempt [see Guide RNA testing in mouse zygotes ]. (bcm.edu)
DNA microinjection method in the pronucleus of zygotes for the generation of transgenic mice, carrying an inserted cassette in their genome. (infrafrontier.gr)
Genome editing in mouse zygotes often results in mosaicism. (bcm.edu)
Traditional microinjection requires manual introduction of genetic material into zygotes thus limiting its efficiency. (cyagen.com)
This system allows large scale transfections of mouse zygotes and eliminates the need for manual injections, thereby, boosting operational efficiency. (cyagen.com)

Transgene-encoded IL-6 mRNA levels were similar in the brain of GFAP-IL6 and GFAP-IL6/sgp130 mice. (jneurosci.org)
This manuscript reports data consistent with a new and unanticipated phenomenon: that Cre or its mRNA may be transmitted between tissues in the mouse and that the male reproductive tract (epididymis) appears to be the most common target of such transported molecules. (elifesciences.org)
Unexpectedly, for all stably-transmitting lines examined, MATR3 transgenic mRNA expression was more robust in muscle, with minimal expression in spinal cord. (biomedcentral.com)
The levels of transgenic mRNA in muscle did not differ between mice from our lead MATR3 F115C line and lead MATR3 WT line, but mice from the lead MATR3 F115C line had significantly higher levels of MATR3 protein in muscle over the lead MATR3 WT line. (biomedcentral.com)

However, the crucial prediction that a disease-associated PrP mutation can spontaneously generate infectivity has only been demonstrated in mice carrying the mutation D177N, the mouse equivalent of the mutation associated with human FFI ( 16 ). (cdc.gov)
In this study, we analyzed the phenotype of transgenic mice expressing mutant 113L bovine prion protein (BoPrP) and the ability of these mice to generate de novo infectious prions in comparison with control mice expressing the wild-type protein. (cdc.gov)
To generate a transgenic mouse, microinjection of DNA or infection with a viral vector containing the gene construct can be used to insert the gene of interest into a zygote or into embryonic stem cells. (promisekit.org)

This genetic disease was transmitted by intracerebral inoculation of brain homogenate from ill mice expressing mutant bovine PrP to mice expressing wild-type bovine PrP, which indicated de novo generation of infectious prions. (cdc.gov)
InfrafronterGR/Phenotypos will provide open access to all newly developed disease models through the European Mouse Mutant Archive (EMMA). (infrafrontier.gr)
Its activities are adapted to the study of mutant mice and genetic reference populations, but can also be used for preclinical studies, for the validation of therapeutic targets, or pharmacological and toxicological studies in mice. (igbmc.fr)
To begin to understand how mutations in MATR3 may cause disease, here we provide initial characterization of transgenic (Tg) mice expressing human wild-type (WT) MATR3 (MATR3 WT ) and ALS-mutant F115C MATR3 (MATR3 F115C ) proteins under the control of the mouse prion promoter (MoPrP). (biomedcentral.com)
To survey the ease with which motor neuron disease (MND) can be transmitted, we injected spinal cord homogenates prepared from paralyzed mice expressing mutant superoxide dismutase 1 (SOD1-G93A and G37R) into the spinal cords of genetically vulnerable SOD1 transgenic mice. (docksci.com)

9] has recently showed that adenovirus-medi- Materials ated in vivo transfer of the PDX-1 transgene to the mouse The PEPCK promoter was generously donated by Dr R. W. liver results in the conversion of a hepatocyte subpopula- Hanson (Case Western Reserve University, Cleveland, tion to the beta cell phenotype. (deepdyve.com)
20 months were not overtly distinguishable from non-transgenic (NT) littermates based on basic motor phenotype. (biomedcentral.com)

This innovative mouse sperm cryorecovery technology builds on experience accumulated over the past years and we expect to reach 10,000 strains by the end of 2018. (cyagen.com)

InfrafrontierGR/Phenotypos is the Greek Research Infrastructure Node for the Molecular, Behavioral and Phenotypic Analysis of Mouse Models for Human Chronic Degenerative Diseases. (infrafrontier.gr)
This double certification concerns our fee-for-service and research and development activities in the following areas: - Genetic engineering for the generation of mouse models - Custom management and experienced breeding of mouse lines -Comprehensive, high-throughput and integrative phenotypic analysis of mouse models. (igbmc.fr)

February 12, 1987) observed inhibition of influenza virus in NIH 3T3 mouse cells genetically modified to express the mouse protein Mx. (justia.com)

OP9 feeder cell co-culture system was used to measure the hematopoietic differentiation from mouse ES and iPS cells. (biomedcentral.com)

The Lund University Transgenic Core Facility (LUTCF) is a core resource whose primary goal is to deliver most up-to-date methodologies in transgenic technology to both our researchers and external users. (lu.se)

Using a pair of black mice from the Granby farm, Little develops the C57BL and C57BR strains. (jax.org)

95% of the animals who fall into this category are rodents, with most of them being mice. (connectusfund.org)
The most common transgenic animals that you will see in the world today are mice that have received genetic modifications so that they naturally produce human antibodies. (connectusfund.org)
The pros and cons of transgenic animals often try to balance the ethics of treating an animal appropriately with the potential benefits that the research can produce. (connectusfund.org)
Some transgenic animals are produced for their specific economic traits. (connectusfund.org)
2. There are three different methods available to use to create transgenic animals. (connectusfund.org)
Most facilities still use the first method, and then the transgenic animals are mated to ensure that their offspring acquire the desired genetic profile. (connectusfund.org)
This advantage allows for the traits to become part of the animals' standard genome over just a generation or two. (connectusfund.org)
Transgenic animals are when one animal carries a different gene from another animal. (sd43.bc.ca)
This course is aimed at technicians and scientists interested in the generation of transgenic animals. (embl.org)
The aim of the course is to provide particpants with hands-on tutorials and the theory behind generating transgenic animals. (embl.org)
Why are mice used for transgenic animals? (promisekit.org)
This invention relates to the production of virus-resistant transgenic animals. (justia.com)
In 2015, approximately 50% of the animals used for scientific procedures in the UK were for the creation and breeding of genetically modified animals, the majority of which are mice (Home Office, 2015). (nc3rs.org.uk)

Methods: To address this question, transgenic Non-Obese Diabetic (NOD) mice which express insulin in the liver were made using the Phosphoenolpyruvate Carboxykinase (PEPCK) promoter to drive the mouse insulin I gene (Ins). (deepdyve.com)

As the leader in custom genetically engineered mouse and rat models, Cyagen has generated thousands of custom knockout, knockin and transgenic rodent lines for its increasing customer base in over 3,000 universities and companies worldwide. (cyagen.com)

generation of genetically modified mice and rats and phenotypic characterization to better understand physio-pathological mechanisms and develop new therapeutics. (igbmc.fr)

After his Nobel Prize winning work elucidating the generation of B-cell and T-cell receptor diversity, Dr. Tonegawa's lab had focused on the cell and molecular biology of memory. (tritechresearch.com)

A transgenic mouse with enhanced viral resistance transmissible to its offspring is prepared by introduction of a gene encoding a human interferon having antiviral activity into a host mouse. (justia.com)
Our invention relates to the introduction of the interferon gene of another animal species into a host animal, and to the production of a healthy transgenic animal with enhanced viral resistance which it can genetically transmit to its offspring. (justia.com)

We are already using transgenic technologies as a way to create insulin and other maintenance medications that are challenging to synthesize otherwise. (connectusfund.org)
furthermore the expression of (pro)insulin in hepatocytes is insufficient to prevent development of diabetes in NOD mice. (deepdyve.com)
person to synthesise, store and secrete insulin in the same manner as a pancreatic β cell is a potential therapy of type The liver of the transgenic PEPCK-Ins NOD mice were 1 diabetes. (deepdyve.com)

Homogenates from paralyzed mice expressing the G37R variant were among those that transmitted poorly regardless of the strain of recipient transgenic animal injected, a finding suggestive of strain-like properties that manifest as differing abilities to transmit MND. (docksci.com)

Background: Transgenesis by microinjection has been widely used for the generation of different mouse models. (uchile.cl)

To facilitate the study of these neurons, transgenic mice were generated that express enhanced green fluorescent protein (EGFP) in subpopulations of GABAergic neurons. (jneurosci.org)
Brain homogenates from MSA or aged control subjects but not saline triggered progressive accumulation of aggregated a-syn in neurons of inoculated mice. (docksci.com)
For these studies we use neurons that are maintained in cell culture as well as genetically-modified strains of mice that are at increased risk for developing neurodegenerative conditions analogous to those observed in humans. (uml.edu)
The key finding of the present study was that the levels of 5-HT released in the PFC by the electrical stimulation of DRN neurons were significantly increased in mice with sciatic nerve ligation. (biomedcentral.com)

Mouse embryonic fibroblasts isolated from transgenic Oct4-GFP reporter mice with or without Sirt6 were used for reprogramming by Yamanaka factors. (biomedcentral.com)

These views were based on a series of methods of her own design, for construction and analysis of chimeric and transgenic mouse models. (pas.va)

We generated transgenic mice expressing bovine cellular prion protein (PrP C ) with a leucine substitution at codon 113 ( 113L ). (cdc.gov)
These mice were able to produce the human protein tPA to treat blood clots. (meddic.jp)
This protein is one whose production is stimulated by mouse interferon. (justia.com)
Tissue homogenates from paralyzed G93A expressing mice induced MND in 6 of 10 mice expressing low levels of G85R-SOD1 fused to yellow fluorescent protein (G85R-YFP mice) by 3- 11 months, and produced widespread spinal inclusion pathology. (docksci.com)

Interpret molecular or physiological alterations of a transgenic animal. (uab.cat)
The lab generates transgenic mice with very cell-type-specific alterations in gene expression to determine the roles of those cells and molecules on memory function, using behavioral testing. (tritechresearch.com)

Germ Line Germ line refers to the sex cells (eggs and sperm) that sexually reproducing organisms use to pass on their genomes from one generation to the next (parents to offspring). (promisekit.org)
The Indiana University Genome Editing Center at Indiana University School of Medicine is an institutionally subsidized shared resource whose mission is to provide Indiana University and external investigators access to state-of-the-art genome editing technologies by assisting with the generation of genetically modified organisms in a time- and cost-effective manner. (iu.edu)

Blastocysts injection method of gene-targeted embryonic stem cells (ESC) for the generation of a new mouse line carrying a disrupted or mutated genetic locus. (infrafrontier.gr)

In one of the resulting transgenic lines, called GIN ( G FP-expressing I nhibitory N eurons), EGFP was found to be expressed in a subpopulation of somatostatin-containing GABAergic interneurons in the hippocampus and neocortex. (jneurosci.org)
Main objective of this call is to facilitate access for the biomedical research community based in Greece, to the unique infrastructure and scientific expertise of the participating InfrafrontierGR/Phenotypos transgenic facilities, to deliver novel mouse lines that will advance knowledge of human disease and will be of widespread use in biomedical science. (infrafrontier.gr)
Furthermore, the transgenic lines developed could be used as a reporter model of innate immunity activation with many different applications in the fields of immunology, cancer and neurodegenerative diseases. (uchile.cl)
For each construct, we established multiple independent lines of mice that stably transmitted the transgene. (biomedcentral.com)
These improvements have not only allowed us to drastically increase the editing rate for our mouse models, but have also resulted in the successful completion of genetically engineered stem cell lines. (iu.edu)

Several transgenic mouse models confirmed that PrP C with mutations induces a spectrum of neurologic diseases with clinical or histologic features of TSEs ( 12 - 15 ). (cdc.gov)
Recent advances in genome editing technology will be used to develop new mouse models of human disease. (infrafrontier.gr)
HSP25 (murine form of HSP27) transgenic (TG) and LLC1-derived orthotropic lung tumor models were also used. (biomedcentral.com)
Lung tissues of patients with RIPF and idiopathic pulmonary fibrosis, and lung tissues from various fibrotic mouse models, as well as appropriated cell line systems were used. (biomedcentral.com)
Mouse anatomy and embryology in order to understand the embryonic development of organs and to analyze morphological/anatomical abnormalities in genetically modified mouse models. (uab.cat)
With the PHENOMIN project launched in 2011 (part of the call for projects entitled National Infrastructures in Biology and Health), the objective of the iCS was to create a national infrastructure specialized in the development, analysis and conservation of mouse models. (igbmc.fr)
To facilitate the access to the engineering, the analysis and the distribution of mouse models for the scientific community. (igbmc.fr)

Open call for development of one novel mouse model of human disease to the biomedical research community based in Greece. (infrafrontier.gr)
The InfrafrontierGR-Phenotypos project supports eligible customers with a free-of-charge mouse model development service implemented as a Trans-regional Access activity supporting a total of two (2) projects in this call. (infrafrontier.gr)
We have developed a mouse model simulating clinical stereotactic body radiotherapy (SBRT) with focal irradiation and validated the induction of RIPF. (biomedcentral.com)
The advantage of such mice is that they provide a model which closely mimics the human body. (promisekit.org)
This AI strategy design tool has significantly accelerated the AI Knockout Mouse Model eBank project. (cyagen.com)
To provide a service platform combining the capacity of generating, on a large-scale basis, mutations in the mouse genome with high-throughput and comprehensive phenotypic analysis of the model. (igbmc.fr)
The Jackson Laboratory has made fundamental contributions to biomedical research, including cancer genetics and establishing the mouse as the premier research animal model. (jax.org)
used that method to produce a mouse model of malignant melanoma resembling the human disease, so as to explore possible treatments. (pas.va)
it shares all of the physical characteristics that make X. laevis a useful model but has the advantage of a diploid genome and short generation time. (biomedcentral.com)

Blockade of trans-signaling in the brain of the GFAP-IL6 mice significantly attenuated Serpina3n but not SOCS3 gene expression, whereas vascular changes including angiogenesis and blood-brain barrier leakage as well as gliosis were also reduced significantly. (jneurosci.org)
On-chip mouse embryo culture: evaluation of effects of uterine cells-conditioned media on embryo development and gene expression. (nc3rs.org.uk)

Harvard scientists received a U.S. patent for a genetically-engineered mouse that carries a gene which promotes the development of human cancer so it can be studied more effectively. (connectusfund.org)

Our findings demonstrate that a single amino acid change in the PrP C sequence can induce spontaneous generation of an infectious prion disease that differs from all others identified in hosts expressing the same PrP C sequence. (cdc.gov)
Although bovine PrP C with the 113L mutation has not been found in nature, it would be useful to establish whether this mutation could induce spontaneous generation of an infectious prion disease in a bovine PrP context. (cdc.gov)
We investigated prion-like propagation of pathological a-syn in Tg(SNCA)1Nbm/J mice that do not express mouse but low levels of human wt a-syn and do not naturally develop any pathology or neurodegenerative disease. (docksci.com)

Which is the first method used to produce the transgenic mice? (promisekit.org)

The first genetically modified animal was a transgenic mouse, created in 1974. (mongabay.com)
This allows us to significantly reduce the number of mice used and increase animal welfare. (cyagen.com)

Spontaneous appearance of infectivity has also been reported in transgenic mice expressing a mouse PrP C with 2 point mutations (170N and 174T) that subtly affect the structure of its globular domain ( 17 ). (cdc.gov)

Apparently, Kawade had not determined whether the chimeric gene was actually expressed in the mice, but had already been able to express it in cultured cells induced by Cd.sup.2+ or by heat. (justia.com)

In October 2021, we held a webinar highlighting the IVF micro microfluidic device, developed to address the EASE Challenge, which enables non-surgical embryo transfer for generating transgenic mice. (nc3rs.org.uk)
Mouse embryo assay to evaluate polydimethylsiloxane embryo-toxicity. (nc3rs.org.uk)
A team led by Dr Virginia Pensabene from the University of Leeds has been awarded £95,883 to deliver the project: Design, Fabrication and Testing of a Mouse Embryo Culture Chip. (nc3rs.org.uk)
She produced chimeric mice (which she at first termed 'allophenic') by inclusion of two genetically different cells in the early mouse embryo, thereby revealing the clonal organization. (pas.va)
found that mouse teratocarcinoma stem cells developed normally in a normal embryo environment. (pas.va)

Bovine P 113L , which has a leucine substitution at codon 113, is homologous to human P 102L and mouse P 101L . (cdc.gov)
The novel mouse line should advance knowledge of human disease and should be of widespread use in biomedical science. (infrafrontier.gr)
Bigenic mice (termed GFAP-IL6/sgp130 mice) were generated with CNS-restricted, astrocyte-targeted production of IL-6 and coproduction of the specific inhibitor of IL-6 trans-signaling, human sgp130-Fc. (jneurosci.org)
Preferably, the human beta interferon gene is introduced into the cells of a mouse. (justia.com)
These services help the scientific community to use the mouse to develop a complete functional annotation of the human genome and thus to better understand human diseases, their physiological and pathological mechanisms. (igbmc.fr)
By joining the International Mouse Phenotyping Consortium , the ICS contributes to decipher mammalian gene functions in order to better understand human physiology and disease. (igbmc.fr)
Intriguingly, brain homogenate from aged control subjects without neurological disorder equally induced synucleinopathy in brains of Tg(SNCA)1Nbm/J mice suggesting that aged human brains can contain pathological a-syn. (docksci.com)
There has been one previous report showing that overexpression of Sirt6 in aged human dermal fibroblasts could improve iPS generation via regulation of miR-766 transcription [ 19 ]. (biomedcentral.com)

The duration of NREM sleep episodes was significantly decreased during photostimulation in these mice. (biomedcentral.com)
We found that the 5-HT levels in the prefrontal cortex (PFC) were significantly increased in nerve-ligated mice compared to those in sham-operated mice after electrical stimulation of the DRN. (biomedcentral.com)

The data serve as a timely warning to mouse researchers about an unexpected complication of Cre-mediated gene manipulation. (elifesciences.org)

The development of tools to manipulate the mouse genome, including knockout and transgenic technology, has revolutionized our ability to explore gene function in mammals. (elifesciences.org)

These mice were generated as a laboratory tool to better understand the onset and progression of cancer. (promisekit.org)

Our findings show that brain homogenate from MSA patients but not saline induces pathological changes in the CNS of Tg(SNCA) 1Nbm/J mice. (docksci.com)
A high-fat and high-cholesterol diet induces overt and latent oxidative damage in hippocampus of mice lacking apolipoprotein E. Open Neuro 12 , 12-18. (uml.edu)

Mice that are widely used for genetic studies don't have a fovea and so studying in albinism in mice may not shed light on the link between albinism and impaired vision. (mongabay.com)

The course will be split into two groups (mammalian cells OR mice) based on participants' expertise. (sb-3.org)

Access to this free-of-charge-service will be granted mainly on the basis of the applicant's research plans and the potential impact of the proposed novel mouse line on the wider biomedical research community. (infrafrontier.gr)
Breeding of chimeras result in the generation of the F1 mutated founder line. (infrafrontier.gr)
What is a transgenic mouse line? (promisekit.org)
DNA microinjection does not have a high success rate (roughly 2% of all injected subjects), even if the new DNA is incorporated in the genome, if it is not accepted by the germ-line the new traits will not appear in their offspring. (meddic.jp)

iPSC Generation is a complex process that reprograms adult somatic cells into a pluripotent, embryonic stem cell-like stage. (cellsystems.eu)
Mouse somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc. (biomedcentral.com)
In this study, we provide evidence that Sirt6 is involved in mouse somatic reprogramming. (biomedcentral.com)
However, whether and how other sirtuins, especially nuclear epigenetic regulator Sirt6, regulate mouse somatic reprogramming still remains exclusive. (biomedcentral.com)
But the exact role of Sirt6 in mouse somatic reprogramming and iPS cell differentiation remains unrevealed. (biomedcentral.com)
In this study, we sought to determine the role of Sirt6 in mouse somatic reprogramming. (biomedcentral.com)

In mice with nerve ligation, we confirmed an increase in wakefulness and a decrease in non-rapid eye movement (NREM) sleep as monitored by electroencephalogram (EEG). (biomedcentral.com)

We use antibodies from mice and other rodents to create new medical therapies that can reduce or eliminate the impact of illness and disease. (connectusfund.org)

Agriculturally, transgenic cows are made that produce more milk and milk with less lactose and cholesterol. (sd43.bc.ca)

Anatomy52

Organisms19

Diseases14

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Psychiatry and Psychology15

Phenomena and Processes54

Disciplines and Occupations1

Information Science3

Health Care1

Producing transgenic animals2

CRISPR9

Production of transgenic2

Double transgenic2

Genes10

Embryos12

Knockout Mice1

Zygotes5

MRNA4

Generate3

Mutant5

Phenotype2

20181

Phenotypic Analys2

19871

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Methodologies1

C57BL1

Animals13

Promoter1

Rodent1

Characterization1

Cell and mol1

Viral2

Insulin3

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Transgenesis1

Neurons4

Reporter mice1

Methods1

Protein4

Alterations2

Organisms2

Embryonic stem1

Lines5

Models7

Model9

Expression2

Genetically-engineered mouse1

Prion3

Method1

Animal2

Mutations1

Express1

Embryo5

Human8

Significantly2

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Widely1

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Line4

Somatic6

Ligation1

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Produce1